AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070968


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070968.t1 MTR_1g102570 91.011 178 14 1 20 195 21 198 8.38e-108 307
MS.gene070968.t1 MTR_1g102800 91.011 178 14 1 20 195 20 197 2.17e-107 306
MS.gene070968.t1 MTR_1g102780 88.333 180 17 1 20 195 21 200 1.43e-101 291
MS.gene070968.t1 MTR_1g102810 76.111 180 31 2 17 195 14 182 2.04e-94 273
MS.gene070968.t1 MTR_1g102830 77.966 177 27 2 20 195 21 186 2.19e-94 273
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070968.t1 AT3G22840 51.256 199 89 3 1 195 1 195 8.32e-60 186
MS.gene070968.t1 AT4G14690 49.510 204 83 5 1 195 1 193 2.55e-56 176

Find 54 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTCAATGAGCACCATTTC+TGG 0.210857 1.4:-79444169 MS.gene070968:CDS
TGGTGGCATCCCTTGGTTCT+TGG 0.234840 1.4:-79443577 MS.gene070968:CDS
GGTGGCATCCCTTGGTTCTT+GGG 0.263333 1.4:-79443576 MS.gene070968:CDS
GGTTTGTTTGATCAAATATA+TGG 0.294584 1.4:-79443600 MS.gene070968:CDS
GGTGATGATGATGGTGTAAT+TGG 0.299898 1.4:+79443892 None:intergenic
ATCCCTTGGTTCTTGGGGAC+AGG 0.355406 1.4:-79443570 MS.gene070968:CDS
CAGAAGGGATGTTGCTGAAT+TGG 0.358772 1.4:+79444131 None:intergenic
TATGTTGGGTTTGATTGCTT+TGG 0.370659 1.4:-79443436 MS.gene070968:CDS
AATGGAAGGTTGGCTATGAT+TGG 0.393839 1.4:-79443663 MS.gene070968:CDS
GGCCTTTACAGAGTATGTTA+AGG 0.395379 1.4:-79443415 MS.gene070968:CDS
GCCTTTACAGAGTATGTTAA+GGG 0.401917 1.4:-79443414 MS.gene070968:CDS
CCATTGATTCTCTCAGGTGC+TGG 0.411549 1.4:+79443681 None:intergenic
TGCCATCAAGTCCGAAAACT+TGG 0.420630 1.4:+79443713 None:intergenic
AACCTTCCATTGATTCTCTC+AGG 0.436061 1.4:+79443675 None:intergenic
GACTCTAGACTTGCCAGAAA+TGG 0.452328 1.4:+79444156 None:intergenic
GTGGCATCCCTTGGTTCTTG+GGG 0.453269 1.4:-79443575 MS.gene070968:CDS
TGTGGTGTTGGTGATGATGA+TGG 0.453563 1.4:+79443883 None:intergenic
GAATGGAAGAATTGCTATGT+TGG 0.460944 1.4:-79443451 MS.gene070968:CDS
GTGATGATGATGGTGTAATT+GGG 0.461935 1.4:+79443893 None:intergenic
TACCTTTGGTGCGCGAGTAT+AGG 0.465801 1.4:+79443855 None:intergenic
TCGGACTTGATGGCATTTGG+TGG 0.487791 1.4:-79443705 MS.gene070968:CDS
AATGGAAGAATTGCTATGTT+GGG 0.499743 1.4:-79443450 MS.gene070968:CDS
CGGACTTGATGGCATTTGGT+GGG 0.500178 1.4:-79443704 MS.gene070968:CDS
GTATAGGCTGGTTGTGGTGT+TGG 0.513497 1.4:+79443871 None:intergenic
CCAGCACCTGAGAGAATCAA+TGG 0.516939 1.4:-79443681 MS.gene070968:CDS
AGTGTTGAGTCCAAGTCAAA+AGG 0.522475 1.4:-79443501 MS.gene070968:CDS
TTTGGTGCGCGAGTATAGGC+TGG 0.530750 1.4:+79443859 None:intergenic
TCAGATGCTGAGCTGTGGAA+TGG 0.542277 1.4:-79443468 MS.gene070968:CDS
AACCTTAATTACCTCAGCCA+TGG 0.545130 1.4:+79444063 None:intergenic
GGTGGAGATAGCAAATGGAC+AGG 0.547189 1.4:-79443622 MS.gene070968:CDS
ATAGCATTCCTTCTGAAGCT+AGG 0.551512 1.4:+79444100 None:intergenic
TATATGGTGGTGGCATCCCT+TGG 0.552085 1.4:-79443584 MS.gene070968:CDS
ACCCTTAACATACTCTGTAA+AGG 0.552111 1.4:+79443413 None:intergenic
TGAGAGAATCAATGGAAGGT+TGG 0.560259 1.4:-79443673 MS.gene070968:CDS
GATGGTGTAATTGGGTCTAC+AGG 0.568013 1.4:+79443901 None:intergenic
TTTGATCAAATATATGGTGG+TGG 0.569010 1.4:-79443594 MS.gene070968:CDS
AAGCTAGGCATGTACACAGA+AGG 0.579754 1.4:+79444115 None:intergenic
TGGACTCAACACTAACACCT+TGG 0.588832 1.4:+79443511 None:intergenic
AGCCTATACTCGCGCACCAA+AGG 0.589710 1.4:-79443857 MS.gene070968:intron
TTGTTTGATCAAATATATGG+TGG 0.590396 1.4:-79443597 MS.gene070968:CDS
TGTCCTCAGATGCTGAGCTG+TGG 0.594735 1.4:-79443473 MS.gene070968:CDS
TATCTTGAAAGTTCGATCCA+TGG 0.599815 1.4:-79444080 MS.gene070968:CDS
GCGCGAGTATAGGCTGGTTG+TGG 0.600268 1.4:+79443865 None:intergenic
CACCTGTCCCCAAGAACCAA+GGG 0.609659 1.4:+79443568 None:intergenic
AGCTAGGCATGTACACAGAA+GGG 0.614155 1.4:+79444116 None:intergenic
ATTCCACAGCTCAGCATCTG+AGG 0.616858 1.4:+79443470 None:intergenic
GAAAGTTCGATCCATGGCTG+AGG 0.622095 1.4:-79444074 MS.gene070968:intron
TGTACATGCCTAGCTTCAGA+AGG 0.622254 1.4:-79444108 MS.gene070968:CDS
ACACCTGTCCCCAAGAACCA+AGG 0.642060 1.4:+79443567 None:intergenic
GTGGAGATAGCAAATGGACA+GGG 0.645295 1.4:-79443621 MS.gene070968:CDS
CACCTGAGAGAATCAATGGA+AGG 0.646646 1.4:-79443677 MS.gene070968:CDS
TCCCTATAGAAATCTACACA+AGG 0.653374 1.4:+79443385 None:intergenic
CCCTATAGAAATCTACACAA+GGG 0.666479 1.4:+79443386 None:intergenic
TATAGAAATCTACACAAGGG+AGG 0.702658 1.4:+79443389 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGATAAACTAAATTTA+AGG - chr1.4:79443558-79443577 MS.gene070968:CDS 10.0%
!!! TTTATGTATAGTATTGATTT+TGG - chr1.4:79443806-79443825 MS.gene070968:intron 15.0%
!! ACATAATATGTTTATGATTC+AGG - chr1.4:79443650-79443669 MS.gene070968:CDS 20.0%
!! GAATCATAAACATATTATGT+TGG + chr1.4:79443650-79443669 None:intergenic 20.0%
! ATCGTCATTATTCAATGAAA+GGG + chr1.4:79443847-79443866 None:intergenic 25.0%
! TATCGTCATTATTCAATGAA+AGG + chr1.4:79443848-79443867 None:intergenic 25.0%
! TTGTTTGATCAAATATATGG+TGG - chr1.4:79444003-79444022 MS.gene070968:intron 25.0%
!! GGTTTGTTTGATCAAATATA+TGG - chr1.4:79444000-79444019 MS.gene070968:intron 25.0%
AATGGAAGAATTGCTATGTT+GGG - chr1.4:79444150-79444169 MS.gene070968:CDS 30.0%
TTTGATCAAATATATGGTGG+TGG - chr1.4:79444006-79444025 MS.gene070968:intron 30.0%
!! TCTTTGATTCCATTTTTCCA+AGG - chr1.4:79444072-79444091 MS.gene070968:intron 30.0%
ACACTAACACCTTGGAAAAA+TGG + chr1.4:79444084-79444103 None:intergenic 35.0%
ACCCTTAACATACTCTGTAA+AGG + chr1.4:79444190-79444209 None:intergenic 35.0%
AGAACAGTTATGCTTACCTT+TGG + chr1.4:79443762-79443781 None:intergenic 35.0%
GAATGGAAGAATTGCTATGT+TGG - chr1.4:79444149-79444168 MS.gene070968:CDS 35.0%
GCCTTTACAGAGTATGTTAA+GGG - chr1.4:79444186-79444205 MS.gene070968:CDS 35.0%
TATCTTGAAAGTTCGATCCA+TGG - chr1.4:79443520-79443539 MS.gene070968:CDS 35.0%
TATGTTGGGTTTGATTGCTT+TGG - chr1.4:79444164-79444183 MS.gene070968:CDS 35.0%
TGAGCAAAAAGAGCAATCAA+AGG - chr1.4:79443674-79443693 MS.gene070968:CDS 35.0%
! GGTGATGATGATTGTGTAAT+CGG + chr1.4:79443629-79443648 None:intergenic 35.0%
!! GTGATGATGATGGTGTAATT+GGG + chr1.4:79443710-79443729 None:intergenic 35.0%
!!! ATTGGTTTTGTAGCAGCAAT+GGG - chr1.4:79443955-79443974 MS.gene070968:intron 35.0%
AACCTTAATTACCTCAGCCA+TGG + chr1.4:79443540-79443559 None:intergenic 40.0%
ATCCATGGCTGAGGTAATTA+AGG - chr1.4:79443535-79443554 MS.gene070968:CDS 40.0%
GGCCTTTACAGAGTATGTTA+AGG - chr1.4:79444185-79444204 MS.gene070968:CDS 40.0%
TGAGAGAATCAATGGAAGGT+TGG - chr1.4:79443927-79443946 MS.gene070968:CDS 40.0%
! AATGGAAGGTTGGCTATGAT+TGG - chr1.4:79443937-79443956 MS.gene070968:intron 40.0%
! ACAGATGAGCACCAAGTTTT+CGG - chr1.4:79443876-79443895 MS.gene070968:CDS 40.0%
! AGTGTTGAGTCCAAGTCAAA+AGG - chr1.4:79444099-79444118 MS.gene070968:CDS 40.0%
!! AACCTTCCATTGATTCTCTC+AGG + chr1.4:79443928-79443947 None:intergenic 40.0%
!! ATAGCATTCCTTCTGAAGCT+AGG + chr1.4:79443503-79443522 None:intergenic 40.0%
!! GGTGATGATGATGGTGTAAT+TGG + chr1.4:79443711-79443730 None:intergenic 40.0%
!! TTTTCGGACTTGATGGCATT+TGG - chr1.4:79443892-79443911 MS.gene070968:CDS 40.0%
!!! GATTGGTTTTGTAGCAGCAA+TGG - chr1.4:79443954-79443973 MS.gene070968:intron 40.0%
!!! TTGGTTTTGTAGCAGCAATG+GGG - chr1.4:79443956-79443975 MS.gene070968:intron 40.0%
AAGCTAGGCATGTACACAGA+AGG + chr1.4:79443488-79443507 None:intergenic 45.0%
AGCTAGGCATGTACACAGAA+GGG + chr1.4:79443487-79443506 None:intergenic 45.0%
AGCTCAATGAGCACCATTTC+TGG - chr1.4:79443431-79443450 MS.gene070968:CDS 45.0%
CACCTGAGAGAATCAATGGA+AGG - chr1.4:79443923-79443942 MS.gene070968:CDS 45.0%
CAGAAGGGATGTTGCTGAAT+TGG + chr1.4:79443472-79443491 None:intergenic 45.0%
GACTCTAGACTTGCCAGAAA+TGG + chr1.4:79443447-79443466 None:intergenic 45.0%
GATGGTGTAATTGGGTCTAC+AGG + chr1.4:79443702-79443721 None:intergenic 45.0%
GTGGAGATAGCAAATGGACA+GGG - chr1.4:79443979-79443998 MS.gene070968:intron 45.0%
TGCCATCAAGTCCGAAAACT+TGG + chr1.4:79443890-79443909 None:intergenic 45.0%
TGGACTCAACACTAACACCT+TGG + chr1.4:79444092-79444111 None:intergenic 45.0%
TGTACATGCCTAGCTTCAGA+AGG - chr1.4:79443492-79443511 MS.gene070968:CDS 45.0%
! CACCAAGTTTTCGGACTTGA+TGG - chr1.4:79443885-79443904 MS.gene070968:CDS 45.0%
!! GGACATGACACCTTTTGACT+TGG + chr1.4:79444112-79444131 None:intergenic 45.0%
!! TGTGGTGTTGGTGATGATGA+TGG + chr1.4:79443720-79443739 None:intergenic 45.0%
!!! TGGTTTTGTAGCAGCAATGG+GGG - chr1.4:79443957-79443976 MS.gene070968:intron 45.0%
ATGGGGGTGGAGATAGCAAA+TGG - chr1.4:79443973-79443992 MS.gene070968:intron 50.0%
ATTCCACAGCTCAGCATCTG+AGG + chr1.4:79444133-79444152 None:intergenic 50.0%
CCAGCACCTGAGAGAATCAA+TGG - chr1.4:79443919-79443938 MS.gene070968:CDS 50.0%
GAAAGTTCGATCCATGGCTG+AGG - chr1.4:79443526-79443545 MS.gene070968:CDS 50.0%
GGTGGAGATAGCAAATGGAC+AGG - chr1.4:79443978-79443997 MS.gene070968:intron 50.0%
TCAGATGCTGAGCTGTGGAA+TGG - chr1.4:79444132-79444151 MS.gene070968:CDS 50.0%
! CGGACTTGATGGCATTTGGT+GGG - chr1.4:79443896-79443915 MS.gene070968:CDS 50.0%
! GTATAGGCTGGTTGTGGTGT+TGG + chr1.4:79443732-79443751 None:intergenic 50.0%
! TACCTTTGGTGCGCGAGTAT+AGG + chr1.4:79443748-79443767 None:intergenic 50.0%
! TCGGACTTGATGGCATTTGG+TGG - chr1.4:79443895-79443914 MS.gene070968:CDS 50.0%
! TTTTGTAGCAGCAATGGGGG+TGG - chr1.4:79443960-79443979 MS.gene070968:intron 50.0%
!! CCATTGATTCTCTCAGGTGC+TGG + chr1.4:79443922-79443941 None:intergenic 50.0%
!! TATATGGTGGTGGCATCCCT+TGG - chr1.4:79444016-79444035 MS.gene070968:intron 50.0%
ACACCTGTCCCCAAGAACCA+AGG + chr1.4:79444036-79444055 None:intergenic 55.0%
AGCCTATACTCGCGCACCAA+AGG - chr1.4:79443743-79443762 MS.gene070968:intron 55.0%
CACCTGTCCCCAAGAACCAA+GGG + chr1.4:79444035-79444054 None:intergenic 55.0%
TGTCCTCAGATGCTGAGCTG+TGG - chr1.4:79444127-79444146 MS.gene070968:CDS 55.0%
! ATCCCTTGGTTCTTGGGGAC+AGG - chr1.4:79444030-79444049 MS.gene070968:intron 55.0%
! GGTGGCATCCCTTGGTTCTT+GGG - chr1.4:79444024-79444043 MS.gene070968:intron 55.0%
! GTGGCATCCCTTGGTTCTTG+GGG - chr1.4:79444025-79444044 MS.gene070968:intron 55.0%
! TGGTGGCATCCCTTGGTTCT+TGG - chr1.4:79444023-79444042 MS.gene070968:intron 55.0%
! TTTGGTGCGCGAGTATAGGC+TGG + chr1.4:79443744-79443763 None:intergenic 55.0%
! GCGCGAGTATAGGCTGGTTG+TGG + chr1.4:79443738-79443757 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 79443398 79444224 79443398 ID=MS.gene070968
chr1.4 mRNA 79443398 79444224 79443398 ID=MS.gene070968.t1;Parent=MS.gene070968
chr1.4 exon 79444075 79444224 79444075 ID=MS.gene070968.t1.exon1;Parent=MS.gene070968.t1
chr1.4 CDS 79444075 79444224 79444075 ID=cds.MS.gene070968.t1;Parent=MS.gene070968.t1
chr1.4 exon 79443858 79443950 79443858 ID=MS.gene070968.t1.exon2;Parent=MS.gene070968.t1
chr1.4 CDS 79443858 79443950 79443858 ID=cds.MS.gene070968.t1;Parent=MS.gene070968.t1
chr1.4 exon 79443398 79443742 79443398 ID=MS.gene070968.t1.exon3;Parent=MS.gene070968.t1
chr1.4 CDS 79443398 79443742 79443398 ID=cds.MS.gene070968.t1;Parent=MS.gene070968.t1
Gene Sequence

>MS.gene070968

ATGGCTGTATCATCTTATCAATCTATCATGTCAAGCTCAATGAGCACCATTTCTGGCAAGTCTAGAGTCAACCAATTCAGCAACATCCCTTCTGTGTACATGCCTAGCTTCAGAAGGAATGCTATCTTGAAAGTTCGATCCATGGCTGAGGTAATTAAGGTTTTAGATAAACTAAATTTAAGGTTATATACATATATAGTATTTGTTCATGCATAGTTGATTAAGTCACCGATTACACAATCATCATCACCAACATAATATGTTTATGATTCAGGATGAGCAAAAAGAGCAATCAAAGGTGCCTGTAGACCCAATTACACCATCATCATCACCAACACCACAACCAGCCTATACTCGCGCACCAAAGGTAAGCATAACTGTTCTTTCAAAGTTGTTTAGAAGAGATTATTTATGTATAGTATTGATTTTGGTAAAATTTTAAGAGACCCTTTCATTGAATAATGACGATAATAATATGACAGATGAGCACCAAGTTTTCGGACTTGATGGCATTTGGTGGGCCAGCACCTGAGAGAATCAATGGAAGGTTGGCTATGATTGGTTTTGTAGCAGCAATGGGGGTGGAGATAGCAAATGGACAGGGTTTGTTTGATCAAATATATGGTGGTGGCATCCCTTGGTTCTTGGGGACAGGTGTGTTGCTGTCACTTGCTTCTTTGATTCCATTTTTCCAAGGTGTTAGTGTTGAGTCCAAGTCAAAAGGTGTCATGTCCTCAGATGCTGAGCTGTGGAATGGAAGAATTGCTATGTTGGGTTTGATTGCTTTGGCCTTTACAGAGTATGTTAAGGGTACCTCCCTTGTGTAG

Protein sequence

>MS.gene070968.t1

MAVSSYQSIMSSSMSTISGKSRVNQFSNIPSVYMPSFRRNAILKVRSMAEDEQKEQSKVPVDPITPSSSPTPQPAYTRAPKMSTKFSDLMAFGGPAPERINGRLAMIGFVAAMGVEIANGQGLFDQIYGGGIPWFLGTGVLLSLASLIPFFQGVSVESKSKGVMSSDAELWNGRIAMLGLIALAFTEYVKGTSLV