AlfalfaGEDB | MS.gene056876

M. sativa cultivar XinJiangDaYe / MS.gene056876

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056876.t1	KEH42025.1	70.4	115	27	1	27	141	10	117	2.10E-32	148.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056876.t1	Q9LXJ1	69.4	62	18	1	75	136	64	124	4.2e-18	92.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056876.t1	A0A072VV98	70.4	115	27	1	27	141	10	117	1.5e-32	148.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054349	MS.gene056876	0.801898	7.24E-49	-1.69E-46
MS.gene056511	MS.gene056876	0.802104	6.57E-49	-1.69E-46
MS.gene056876	MS.gene059807	0.826835	2.18E-54	-1.69E-46
MS.gene056876	MS.gene064519	0.803502	3.38E-49	-1.69E-46
MS.gene056876	MS.gene066009	0.820138	8.03E-53	-1.69E-46
MS.gene056876	MS.gene068064	0.817073	3.98E-52	-1.69E-46
MS.gene056876	MS.gene068746	0.828953	6.75E-55	-1.69E-46
MS.gene056876	MS.gene069717	0.813503	2.48E-51	-1.69E-46
MS.gene056876	MS.gene070968	0.805884	1.08E-49	-1.69E-46
MS.gene056876	MS.gene071722	0.812555	4.00E-51	-1.69E-46
MS.gene056876	MS.gene072070	0.804904	1.73E-49	-1.69E-46
MS.gene056876	MS.gene26111	0.834203	3.43E-56	-1.69E-46
MS.gene056876	MS.gene27100	0.806416	8.31E-50	-1.69E-46
MS.gene056876	MS.gene30932	0.829026	6.48E-55	-1.69E-46
MS.gene056876	MS.gene32713	0.804312	2.30E-49	-1.69E-46
MS.gene056876	MS.gene34394	0.805448	1.33E-49	-1.69E-46
MS.gene056876	MS.gene34409	0.805121	1.56E-49	-1.69E-46
MS.gene056876	MS.gene34496	0.830173	3.41E-55	-1.69E-46
MS.gene056876	MS.gene35984	0.816325	5.86E-52	-1.69E-46
MS.gene056876	MS.gene36105	0.833352	5.60E-56	-1.69E-46
MS.gene056876	MS.gene36507	0.820382	7.06E-53	-1.69E-46
MS.gene056876	MS.gene36818	0.818138	2.29E-52	-1.69E-46
MS.gene056876	MS.gene41435	0.822129	2.79E-53	-1.69E-46
MS.gene056876	MS.gene42746	0.812297	4.55E-51	-1.69E-46
MS.gene056876	MS.gene42747	0.82292	1.83E-53	-1.69E-46
MS.gene056876	MS.gene43562	0.809245	2.08E-50	-1.69E-46
MS.gene056876	MS.gene44558	0.827594	1.44E-54	-1.69E-46
MS.gene056876	MS.gene46346	0.802412	5.68E-49	-1.69E-46
MS.gene056876	MS.gene51922	0.808792	2.60E-50	-1.69E-46
MS.gene056876	MS.gene52283	0.808341	3.25E-50	-1.69E-46
MS.gene056876	MS.gene52997	0.801419	9.08E-49	-1.69E-46
MS.gene056876	MS.gene63115	0.803128	4.04E-49	-1.69E-46
MS.gene056876	MS.gene68677	0.829906	3.96E-55	-1.69E-46
MS.gene056876	MS.gene71664	0.815186	1.05E-51	-1.69E-46
MS.gene056876	MS.gene75014	0.800767	1.23E-48	-1.69E-46
MS.gene056876	MS.gene77541	0.808461	3.06E-50	-1.69E-46
MS.gene056876	MS.gene78531	0.81423	1.71E-51	-1.69E-46
MS.gene056876	MS.gene78719	0.817186	3.76E-52	-1.69E-46
MS.gene056876	MS.gene89744	0.80962	1.73E-50	-1.69E-46
MS.gene056876	MS.gene90950	0.803643	3.16E-49	-1.69E-46
MS.gene056876	MS.gene93035	0.821329	4.28E-53	-1.69E-46
MS.gene056876	MS.gene95197	0.803094	4.11E-49	-1.69E-46
MS.gene056876	MS.gene95702	0.820354	7.17E-53	-1.69E-46
MS.gene056876	MS.gene97081	0.803012	4.27E-49	-1.69E-46
MS.gene056876	MS.gene97140	0.818184	2.24E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056876.t1	MTR_1g059990	82.258	124	15	1	18	141	1	117	2.32e-70	207
MS.gene056876.t1	MTR_7g104540	42.593	162	49	5	18	136	1	161	3.67e-31	110
MS.gene056876.t1	MTR_2g086480	57.143	63	24	2	73	133	71	132	4.73e-14	67.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056876.t1	AT3G52740	69.355	62	18	1	75	136	64	124	1.02e-25	95.1
MS.gene056876.t1	AT3G44450	38.776	98	52	2	37	134	7	96	6.08e-17	72.0

Find 24 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTCAATTCAATATCAATT+TGG	0.251682	1.1:+45709795	None:intergenic
TCTTCTTGTCCCCACATATC+TGG	0.303455	1.1:+45709596	None:intergenic
CTTTCTTCTTCCTCAACAAT+AGG	0.318419	1.1:+45709674	None:intergenic
CTTTGGTTCAAGAAGCTACT+AGG	0.363188	1.1:-45709493	MS.gene056876:CDS
TTGAAATGGCTGGAAGAGTA+TGG	0.372715	1.1:-45709622	MS.gene056876:CDS
TGTTATCTTTCATGCAAAAC+AGG	0.376927	1.1:+45709873	None:intergenic
AAGAAGAGTATTTGAAGGAT+TGG	0.399170	1.1:-45709580	MS.gene056876:CDS
TGTGACTGCTAGAACAGCTT+TGG	0.411343	1.1:-45709510	MS.gene056876:CDS
TAGGATTCAGATTCCACTAT+AGG	0.435137	1.1:-45709474	None:intergenic
GAAGAGACATAGAGTTGAAA+TGG	0.437286	1.1:-45709636	MS.gene056876:CDS
CATGAGATTTAGTAGATGAT+TGG	0.476408	1.1:+45709819	None:intergenic
TGAAGTTGCATATTTGAAGA+AGG	0.476519	1.1:-45709705	MS.gene056876:CDS
AGACATAGAGTTGAAATGGC+TGG	0.496362	1.1:-45709632	MS.gene056876:CDS
GAGTATGGATTCCAGATATG+TGG	0.497888	1.1:-45709607	MS.gene056876:CDS
GGGACAAGAAGAGTATTTGA+AGG	0.539935	1.1:-45709585	MS.gene056876:CDS
AGTATGGATTCCAGATATGT+GGG	0.543345	1.1:-45709606	MS.gene056876:CDS
TTGTTATCTTTGAGTTGATG+AGG	0.550414	1.1:+45709770	None:intergenic
AGTTGCATATTTGAAGAAGG+TGG	0.555424	1.1:-45709702	MS.gene056876:CDS
AAGAAGAAAGTGGAAGAGAG+AGG	0.561315	1.1:-45709661	MS.gene056876:CDS
AGAGCAACAACAACAAGAAG+AGG	0.565224	1.1:-45709741	MS.gene056876:CDS
ATTGTTGAGGAAGAAGAAAG+TGG	0.598074	1.1:-45709671	MS.gene056876:CDS
TTGCTAGCAGAAATTAAAGG+AGG	0.610220	1.1:+45709536	None:intergenic
GTATGGATTCCAGATATGTG+GGG	0.706622	1.1:-45709605	MS.gene056876:CDS
TGATTGGTGAGTCATTGTAG+TGG	0.742104	1.1:+45709835	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCTTCAATTCAATATCAATT+TGG	+	chr1.1:45709561-45709580	None:intergenic	20.0%
!!	ATTTTGCTAGCAGAAATTAA+AGG	+	chr1.1:45709823-45709842	None:intergenic	25.0%
	CATGAGATTTAGTAGATGAT+TGG	+	chr1.1:45709537-45709556	None:intergenic	30.0%
	TGAAGTTGCATATTTGAAGA+AGG	-	chr1.1:45709648-45709667	MS.gene056876:CDS	30.0%
	TGTTATCTTTCATGCAAAAC+AGG	+	chr1.1:45709483-45709502	None:intergenic	30.0%
!!	AAGAAGAGTATTTGAAGGAT+TGG	-	chr1.1:45709773-45709792	MS.gene056876:CDS	30.0%
!!	TTGTTATCTTTGAGTTGATG+AGG	+	chr1.1:45709586-45709605	None:intergenic	30.0%
	AGTATGGATTCCAGATATGT+GGG	-	chr1.1:45709747-45709766	MS.gene056876:CDS	35.0%
	AGTTGCATATTTGAAGAAGG+TGG	-	chr1.1:45709651-45709670	MS.gene056876:CDS	35.0%
	ATTGTTGAGGAAGAAGAAAG+TGG	-	chr1.1:45709682-45709701	MS.gene056876:CDS	35.0%
	CTTTCTTCTTCCTCAACAAT+AGG	+	chr1.1:45709682-45709701	None:intergenic	35.0%
	GAAGAGACATAGAGTTGAAA+TGG	-	chr1.1:45709717-45709736	MS.gene056876:CDS	35.0%
	TTGCTAGCAGAAATTAAAGG+AGG	+	chr1.1:45709820-45709839	None:intergenic	35.0%
	AAGAAGAAAGTGGAAGAGAG+AGG	-	chr1.1:45709692-45709711	MS.gene056876:CDS	40.0%
	AGACATAGAGTTGAAATGGC+TGG	-	chr1.1:45709721-45709740	MS.gene056876:CDS	40.0%
	AGAGCAACAACAACAAGAAG+AGG	-	chr1.1:45709612-45709631	MS.gene056876:CDS	40.0%
	GAGTATGGATTCCAGATATG+TGG	-	chr1.1:45709746-45709765	MS.gene056876:CDS	40.0%
	GTATGGATTCCAGATATGTG+GGG	-	chr1.1:45709748-45709767	MS.gene056876:CDS	40.0%
	TGATTGGTGAGTCATTGTAG+TGG	+	chr1.1:45709521-45709540	None:intergenic	40.0%
	TTGAAATGGCTGGAAGAGTA+TGG	-	chr1.1:45709731-45709750	MS.gene056876:CDS	40.0%
!	CTTTGGTTCAAGAAGCTACT+AGG	-	chr1.1:45709860-45709879	MS.gene056876:CDS	40.0%
!!	GGGACAAGAAGAGTATTTGA+AGG	-	chr1.1:45709768-45709787	MS.gene056876:CDS	40.0%
	TCTTCTTGTCCCCACATATC+TGG	+	chr1.1:45709760-45709779	None:intergenic	45.0%
	TGTGACTGCTAGAACAGCTT+TGG	-	chr1.1:45709843-45709862	MS.gene056876:CDS	45.0%
!!	GGTGGTTTTGCCTATTGTTG+AGG	-	chr1.1:45709669-45709688	MS.gene056876:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	45709475	45709900	45709475	ID=MS.gene056876
chr1.1	mRNA	45709475	45709900	45709475	ID=MS.gene056876.t1;Parent=MS.gene056876
chr1.1	exon	45709475	45709900	45709475	ID=MS.gene056876.t1.exon1;Parent=MS.gene056876.t1
chr1.1	CDS	45709475	45709900	45709475	ID=cds.MS.gene056876.t1;Parent=MS.gene056876.t1

Gene Sequence

>MS.gene056876

ATGGTCCTGTTTTGCATGAAAGATAACAACACAAACACAAATACCACTACAATGACTCACCAATCATCTACTAAATCTCATGACCAAATTGATATTGAATTGAAGAAACCTCATCAACTCAAAGATAACAATATTAAAGAGCAACAACAACAAGAAGAGGATGACACAAACATTGAAGTTGCATATTTGAAGAAGGTGGTTTTGCCTATTGTTGAGGAAGAAGAAAGTGGAAGAGAGAGGTTGAAGAGACATAGAGTTGAAATGGCTGGAAGAGTATGGATTCCAGATATGTGGGGACAAGAAGAGTATTTGAAGGATTGGATAGATTGCACAACATTTGATCCTCCTTTAATTTCTGCTAGCAAAATTGTGACTGCTAGAACAGCTTTGGTTCAAGAAGCTACTAGGATTCAGATTCCACTATAG

Protein sequence

>MS.gene056876.t1

MVLFCMKDNNTNTNTTTMTHQSSTKSHDQIDIELKKPHQLKDNNIKEQQQQEEDDTNIEVAYLKKVVLPIVEEEESGRERLKRHRVEMAGRVWIPDMWGQEEYLKDWIDCTTFDPPLISASKIVTARTALVQEATRIQIPL