AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071669


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071669.t1 MTR_1g114200 98.913 92 1 0 1 92 23 114 3.35e-61 182
MS.gene071669.t1 MTR_4g032660 33.708 89 59 0 1 89 108 196 1.27e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071669.t1 AT5G27820 78.261 92 20 0 1 92 23 114 2.13e-50 154
MS.gene071669.t1 AT3G20230 35.955 89 57 0 1 89 95 183 1.50e-14 65.5

Find 23 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACATCCCGAGTAGTTTCC+AGG 0.336475 1.3:+77276606 None:intergenic
AAGCGGTTATTGATTCTCTC+AGG 0.400454 1.3:-77276475 MS.gene071669:CDS
GAAAGCCTTGAGATCAAGCC+TGG 0.444737 1.3:-77276624 MS.gene071669:CDS
CTCCTGTGAGCTTGCAGAAG+AGG 0.447057 1.3:+77276646 None:intergenic
ATTGATTCTCTCAGGGAAGC+TGG 0.452667 1.3:-77276467 MS.gene071669:CDS
AAGAGAGAACAGAGGTATCA+TGG 0.461247 1.3:-77276506 MS.gene071669:CDS
GTTGCTGCAGCTGCAAAGAT+TGG 0.469361 1.3:-77276587 MS.gene071669:CDS
AGTTTCCAGGCTTGATCTCA+AGG 0.483054 1.3:+77276619 None:intergenic
TGCCTCTTCTGCAAGCTCAC+AGG 0.512391 1.3:-77276648 MS.gene071669:CDS
GAAGAGGCAACGGTAGCAGT+TGG 0.531232 1.3:+77276662 None:intergenic
GATCAAGCCTGGAAACTACT+CGG 0.540330 1.3:-77276613 MS.gene071669:CDS
AGCGGAACGCCTTTCGCTCA+AGG 0.547714 1.3:-77276555 MS.gene071669:CDS
CAGGAATGTCCTTGAGCGAA+AGG 0.550217 1.3:+77276546 None:intergenic
TTGCTGCAGCTGCAAAGATT+GGG 0.550997 1.3:-77276586 MS.gene071669:CDS
AGCGGTTATTGATTCTCTCA+GGG 0.559363 1.3:-77276474 MS.gene071669:CDS
TTCAAGTGAACAGAAACAGC+AGG 0.564698 1.3:+77276527 None:intergenic
AGAACAGAGGTATCATGGAA+AGG 0.588862 1.3:-77276501 MS.gene071669:CDS
ATCAAGCCTGGAAACTACTC+GGG 0.593151 1.3:-77276612 MS.gene071669:CDS
CAACGGTAGCAGTTGGAGTG+TGG 0.604975 1.3:+77276669 None:intergenic
TGAGCTTGCAGAAGAGGCAA+CGG 0.638179 1.3:+77276652 None:intergenic
GTATCATGGAAAGGTAAAAG+CGG 0.664247 1.3:-77276492 MS.gene071669:CDS
AAAGATTGGGAAGCTACTAG+CGG 0.716694 1.3:-77276573 MS.gene071669:CDS
TTCACTTGAAGAGAGAACAG+AGG 0.737493 1.3:-77276514 MS.gene071669:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GTATCATGGAAAGGTAAAAG+CGG - chr1.3:77276666-77276685 MS.gene071669:CDS 35.0%
AAAGATTGGGAAGCTACTAG+CGG - chr1.3:77276585-77276604 MS.gene071669:CDS 40.0%
TTCAAGTGAACAGAAACAGC+AGG + chr1.3:77276634-77276653 None:intergenic 40.0%
TTCACTTGAAGAGAGAACAG+AGG - chr1.3:77276644-77276663 MS.gene071669:CDS 40.0%
!! AAGAGAGAACAGAGGTATCA+TGG - chr1.3:77276652-77276671 MS.gene071669:CDS 40.0%
!! AAGCGGTTATTGATTCTCTC+AGG - chr1.3:77276683-77276702 MS.gene071669:CDS 40.0%
!! AGAACAGAGGTATCATGGAA+AGG - chr1.3:77276657-77276676 MS.gene071669:CDS 40.0%
!! AGCGGTTATTGATTCTCTCA+GGG - chr1.3:77276684-77276703 MS.gene071669:CDS 40.0%
AGTTTCCAGGCTTGATCTCA+AGG + chr1.3:77276542-77276561 None:intergenic 45.0%
ATCAAGCCTGGAAACTACTC+GGG - chr1.3:77276546-77276565 MS.gene071669:CDS 45.0%
GATCAAGCCTGGAAACTACT+CGG - chr1.3:77276545-77276564 MS.gene071669:CDS 45.0%
TTGCTGCAGCTGCAAAGATT+GGG - chr1.3:77276572-77276591 MS.gene071669:CDS 45.0%
! ATTGATTCTCTCAGGGAAGC+TGG - chr1.3:77276691-77276710 MS.gene071669:CDS 45.0%
CAACATCCCGAGTAGTTTCC+AGG + chr1.3:77276555-77276574 None:intergenic 50.0%
CAGGAATGTCCTTGAGCGAA+AGG + chr1.3:77276615-77276634 None:intergenic 50.0%
GAAAGCCTTGAGATCAAGCC+TGG - chr1.3:77276534-77276553 MS.gene071669:CDS 50.0%
GTTGCTGCAGCTGCAAAGAT+TGG - chr1.3:77276571-77276590 MS.gene071669:CDS 50.0%
TGAGCTTGCAGAAGAGGCAA+CGG + chr1.3:77276509-77276528 None:intergenic 50.0%
CAACGGTAGCAGTTGGAGTG+TGG + chr1.3:77276492-77276511 None:intergenic 55.0%
CTCCTGTGAGCTTGCAGAAG+AGG + chr1.3:77276515-77276534 None:intergenic 55.0%
GAAGAGGCAACGGTAGCAGT+TGG + chr1.3:77276499-77276518 None:intergenic 55.0%
TGCCTCTTCTGCAAGCTCAC+AGG - chr1.3:77276510-77276529 MS.gene071669:CDS 55.0%
AGCGGAACGCCTTTCGCTCA+AGG - chr1.3:77276603-77276622 MS.gene071669:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 77276451 77276729 77276451 ID=MS.gene071669
chr1.3 mRNA 77276451 77276729 77276451 ID=MS.gene071669.t1;Parent=MS.gene071669
chr1.3 exon 77276451 77276729 77276451 ID=MS.gene071669.t1.exon1;Parent=MS.gene071669.t1
chr1.3 CDS 77276451 77276729 77276451 ID=cds.MS.gene071669.t1;Parent=MS.gene071669.t1
Gene Sequence

>MS.gene071669

ATGCATTTCACAAATAAGTATGTGAGTGCTCAAGTGATCCACACTCCAACTGCTACCGTTGCCTCTTCTGCAAGCTCACAGGAGAAAGCCTTGAGATCAAGCCTGGAAACTACTCGGGATGTTGCTGCAGCTGCAAAGATTGGGAAGCTACTAGCGGAACGCCTTTCGCTCAAGGACATTCCTGCTGTTTCTGTTCACTTGAAGAGAGAACAGAGGTATCATGGAAAGGTAAAAGCGGTTATTGATTCTCTCAGGGAAGCTGGTGTTAAGTTGCTTTGA

Protein sequence

>MS.gene071669.t1

MHFTNKYVSAQVIHTPTATVASSASSQEKALRSSLETTRDVAAAAKIGKLLAERLSLKDIPAVSVHLKREQRYHGKVKAVIDSLREAGVKLL