Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074293.t1 | XP_013451289.1 | 94.5 | 274 | 15 | 0 | 1 | 274 | 1 | 274 | 2.00E-136 | 496.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074293.t1 | A0A072U6F4 | 94.5 | 274 | 15 | 0 | 1 | 274 | 1 | 274 | 1.5e-136 | 496.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049351 | MS.gene074293 | 0.800666 | 1.29E-48 | -1.69E-46 |
| MS.gene049614 | MS.gene074293 | 0.814299 | 1.65E-51 | -1.69E-46 |
| MS.gene049661 | MS.gene074293 | 0.833809 | 4.31E-56 | -1.69E-46 |
| MS.gene049662 | MS.gene074293 | 0.830132 | 3.49E-55 | -1.69E-46 |
| MS.gene050076 | MS.gene074293 | 0.821682 | 3.55E-53 | -1.69E-46 |
| MS.gene050397 | MS.gene074293 | 0.800191 | 1.62E-48 | -1.69E-46 |
| MS.gene050432 | MS.gene074293 | 0.80103 | 1.09E-48 | -1.69E-46 |
| MS.gene050767 | MS.gene074293 | 0.815624 | 8.40E-52 | -1.69E-46 |
| MS.gene050770 | MS.gene074293 | 0.81967 | 1.03E-52 | -1.69E-46 |
| MS.gene05101 | MS.gene074293 | 0.82275 | 2.00E-53 | -1.69E-46 |
| MS.gene051692 | MS.gene074293 | 0.809474 | 1.86E-50 | -1.69E-46 |
| MS.gene051877 | MS.gene074293 | 0.816139 | 6.45E-52 | -1.69E-46 |
| MS.gene052271 | MS.gene074293 | 0.808319 | 3.28E-50 | -1.69E-46 |
| MS.gene052290 | MS.gene074293 | 0.808212 | 3.46E-50 | -1.69E-46 |
| MS.gene052326 | MS.gene074293 | 0.834771 | 2.47E-56 | -1.69E-46 |
| MS.gene053322 | MS.gene074293 | 0.840071 | 1.08E-57 | -1.69E-46 |
| MS.gene053813 | MS.gene074293 | 0.811616 | 6.40E-51 | -1.69E-46 |
| MS.gene054376 | MS.gene074293 | 0.804087 | 2.56E-49 | -1.69E-46 |
| MS.gene054802 | MS.gene074293 | 0.81907 | 1.41E-52 | -1.69E-46 |
| MS.gene054803 | MS.gene074293 | 0.81767 | 2.92E-52 | -1.69E-46 |
| MS.gene054804 | MS.gene074293 | 0.820112 | 8.15E-53 | -1.69E-46 |
| MS.gene055277 | MS.gene074293 | 0.823635 | 1.25E-53 | -1.69E-46 |
| MS.gene055499 | MS.gene074293 | 0.844483 | 7.35E-59 | -1.69E-46 |
| MS.gene056191 | MS.gene074293 | 0.850569 | 1.56E-60 | -1.69E-46 |
| MS.gene056410 | MS.gene074293 | 0.822545 | 2.24E-53 | -1.69E-46 |
| MS.gene056931 | MS.gene074293 | 0.812778 | 3.57E-51 | -1.69E-46 |
| MS.gene056933 | MS.gene074293 | 0.807981 | 3.88E-50 | -1.69E-46 |
| MS.gene056935 | MS.gene074293 | 0.811697 | 6.15E-51 | -1.69E-46 |
| MS.gene056942 | MS.gene074293 | 0.811655 | 6.28E-51 | -1.69E-46 |
| MS.gene057146 | MS.gene074293 | 0.810174 | 1.31E-50 | -1.69E-46 |
| MS.gene05733 | MS.gene074293 | 0.812436 | 4.24E-51 | -1.69E-46 |
| MS.gene058660 | MS.gene074293 | 0.84711 | 1.42E-59 | -1.69E-46 |
| MS.gene059536 | MS.gene074293 | 0.826693 | 2.36E-54 | -1.69E-46 |
| MS.gene059688 | MS.gene074293 | 0.804775 | 1.84E-49 | -1.69E-46 |
| MS.gene059692 | MS.gene074293 | 0.800994 | 1.11E-48 | -1.69E-46 |
| MS.gene059695 | MS.gene074293 | 0.800616 | 1.33E-48 | -1.69E-46 |
| MS.gene06037 | MS.gene074293 | 0.801756 | 7.75E-49 | -1.69E-46 |
| MS.gene060559 | MS.gene074293 | 0.806184 | 9.30E-50 | -1.69E-46 |
| MS.gene060804 | MS.gene074293 | 0.813649 | 2.30E-51 | -1.69E-46 |
| MS.gene061200 | MS.gene074293 | 0.828277 | 9.83E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074293.t1 | MTR_6g017240 | 96.715 | 274 | 9 | 0 | 1 | 274 | 1 | 274 | 0.0 | 543 |
| MS.gene074293.t1 | MTR_7g018370 | 64.706 | 272 | 86 | 6 | 8 | 274 | 6 | 272 | 1.13e-104 | 317 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074293.t1 | AT5G16030 | 54.310 | 232 | 85 | 7 | 14 | 229 | 2 | 228 | 1.06e-63 | 209 |
| MS.gene074293.t1 | AT3G02500 | 63.333 | 150 | 49 | 3 | 15 | 163 | 3 | 147 | 1.83e-56 | 191 |
| MS.gene074293.t1 | AT3G02500 | 63.333 | 150 | 49 | 3 | 15 | 163 | 3 | 147 | 1.88e-56 | 191 |
| MS.gene074293.t1 | AT5G16030 | 46.333 | 300 | 130 | 9 | 14 | 288 | 2 | 295 | 2.56e-56 | 192 |
| MS.gene074293.t1 | AT5G16030 | 45.779 | 308 | 128 | 9 | 14 | 288 | 2 | 303 | 1.98e-55 | 190 |
| MS.gene074293.t1 | AT5G16030 | 46.179 | 301 | 130 | 10 | 14 | 288 | 2 | 296 | 1.17e-54 | 188 |
| MS.gene074293.t1 | AT3G02500 | 55.333 | 150 | 46 | 4 | 15 | 163 | 3 | 132 | 3.60e-43 | 155 |
| MS.gene074293.t1 | AT5G16030 | 39.524 | 210 | 102 | 5 | 99 | 288 | 1 | 205 | 5.12e-23 | 98.6 |
Find 76 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATATATGCAACCCTCTTTC+AGG | 0.198253 | 6.2:+13421769 | MS.gene074293:CDS |
| TCTTTCACTACTGCTCTTAA+AGG | 0.203772 | 6.2:+13420926 | MS.gene074293:CDS |
| TCTCCTGGATATACATTAAA+AGG | 0.205126 | 6.2:+13420765 | MS.gene074293:CDS |
| GTTCAATTAAACTCAGTATT+TGG | 0.206884 | 6.2:-13435724 | None:intergenic |
| TAGTCCTTGCAATTGGTTAT+TGG | 0.242653 | 6.2:-13434482 | None:intergenic |
| GATTTGCATTAAATTCTAAA+TGG | 0.262311 | 6.2:+13421731 | MS.gene074293:CDS |
| AATAAAATCATTCTTCCTAA+TGG | 0.292897 | 6.2:+13429191 | MS.gene074293:CDS |
| TCTTGTTTCATTGAACTATA+AGG | 0.296631 | 6.2:-13420585 | None:intergenic |
| AAACACCACCAAAACGGATA+TGG | 0.312867 | 6.2:-13433415 | None:intergenic |
| CAAGATCAGTTTACAAAAGT+TGG | 0.315706 | 6.2:+13421689 | MS.gene074293:CDS |
| TCATCAACCATTGTTAATAA+TGG | 0.330556 | 6.2:+13435555 | MS.gene074293:CDS |
| AATCGGCGCCATCAAGAAAA+AGG | 0.353968 | 6.2:+13429672 | MS.gene074293:CDS |
| ATGCTCATCCATATCCGTTT+TGG | 0.357572 | 6.2:+13433407 | MS.gene074293:CDS |
| TCTAGCACTGCTTCTTCTCC+TGG | 0.359111 | 6.2:+13420750 | MS.gene074293:CDS |
| AGAGTTCAATTTAACAGAAT+GGG | 0.365032 | 6.2:+13420614 | MS.gene074293:CDS |
| CTGCTTGTAGTCCTTGCAAT+TGG | 0.388353 | 6.2:-13434489 | None:intergenic |
| TTAGTGGGGATTTGTTCATT+TGG | 0.392740 | 6.2:+13429570 | MS.gene074293:intron |
| TAAATTGATTGAAGATGAAA+TGG | 0.401835 | 6.2:+13433445 | MS.gene074293:CDS |
| TTGCAACTTTGAACATGCTT+TGG | 0.417567 | 6.2:-13433383 | None:intergenic |
| CAGAGTTCAATTTAACAGAA+TGG | 0.417830 | 6.2:+13420613 | MS.gene074293:CDS |
| AAGATCAGTTTACAAAAGTT+GGG | 0.421442 | 6.2:+13421690 | MS.gene074293:CDS |
| AAGAGCAGTAGTGAAAGAAT+AGG | 0.426995 | 6.2:-13420921 | None:intergenic |
| GAATTTAATGCAAATCCATC+TGG | 0.427834 | 6.2:-13421724 | None:intergenic |
| TATTTGAAAGGTCTCGCAAT+CGG | 0.446438 | 6.2:+13429655 | MS.gene074293:CDS |
| TGTCTTGATGAAAAGACTAA+AGG | 0.455118 | 6.2:-13421880 | None:intergenic |
| GATGGAGTTAAATTAGTGCT+TGG | 0.456349 | 6.2:-13422544 | None:intergenic |
| GCTCTTCAATTTCATAGTCC+AGG | 0.458142 | 6.2:+13421955 | MS.gene074293:CDS |
| AGAAGAAGCAGTGCTAGAAA+TGG | 0.469708 | 6.2:-13420745 | None:intergenic |
| TTGCAACATTCTTATTTGAA+AGG | 0.484617 | 6.2:+13429643 | MS.gene074293:CDS |
| AATGGATCTTGAAGAAACTA+AGG | 0.489811 | 6.2:+13433463 | MS.gene074293:CDS |
| ATATATGCAACCCTCTTTCA+GGG | 0.494830 | 6.2:+13421770 | MS.gene074293:CDS |
| TAACTAAGAGTTTATATGTC+GGG | 0.494918 | 6.2:+13435622 | MS.gene074293:CDS |
| ATTAAATTCTAAATGGAATG+AGG | 0.495126 | 6.2:+13421738 | MS.gene074293:CDS |
| GTAGTGAAAGAATAGGTTGA+AGG | 0.505121 | 6.2:-13420914 | None:intergenic |
| GTCCGACTCAGGCACAAGGC+GGG | 0.512509 | 6.2:-13429135 | None:intergenic |
| TCAAACTAAATCTGAGGAAG+AGG | 0.515168 | 6.2:+13422615 | MS.gene074293:CDS |
| TCTTCCTCATCGTCCGACTC+AGG | 0.520585 | 6.2:-13429146 | None:intergenic |
| ATTGGTACTTCCCCTGAAAG+AGG | 0.520887 | 6.2:-13421781 | None:intergenic |
| TTTGCAGATAAACATTCCAT+TGG | 0.521868 | 6.2:-13421799 | None:intergenic |
| ATCATCCTCATCGTCAGACT+CGG | 0.529812 | 6.2:-13431282 | None:intergenic |
| AAAACACATGGCTTCCACAT+TGG | 0.533224 | 6.2:+13420548 | None:intergenic |
| GTTGCAATGATACGAATTGT+CGG | 0.536166 | 6.2:-13429627 | None:intergenic |
| GATGAACAACTTTGCAGACA+AGG | 0.536730 | 6.2:+13424011 | MS.gene074293:CDS |
| ATTTGTTGACATCTCTACAA+TGG | 0.537047 | 6.2:-13422566 | None:intergenic |
| CACTAGTAGTGGGAGTGCAA+TGG | 0.544695 | 6.2:-13435670 | None:intergenic |
| TGGGAGTGTTTATCACCAGA+TGG | 0.544959 | 6.2:+13421709 | MS.gene074293:CDS |
| TCACATAAACACCACCAAAA+CGG | 0.547389 | 6.2:-13433421 | None:intergenic |
| TTGTGTACCATTATTAACAA+TGG | 0.553442 | 6.2:-13435562 | None:intergenic |
| CAATGATACGAATTGTCGGT+TGG | 0.553574 | 6.2:-13429623 | None:intergenic |
| CATTGCACTCCCACTACTAG+TGG | 0.561848 | 6.2:+13435671 | MS.gene074293:CDS |
| AGCCCGCCTTGTGCCTGAGT+CGG | 0.564566 | 6.2:+13429133 | MS.gene074293:CDS |
| GAGTTCAATTTAACAGAATG+GGG | 0.572131 | 6.2:+13420615 | MS.gene074293:CDS |
| TGGAAGCTAGAACATAACTC+TGG | 0.572441 | 6.2:-13435650 | None:intergenic |
| TTGGTACTTCCCCTGAAAGA+GGG | 0.575017 | 6.2:-13421780 | None:intergenic |
| GTTGACATCTCTACAATGGA+TGG | 0.575450 | 6.2:-13422562 | None:intergenic |
| GTGGACATCAAAATTTGTGA+AGG | 0.577473 | 6.2:+13435690 | MS.gene074293:CDS |
| AAAGACTAAAGGAGCTGACA+TGG | 0.580020 | 6.2:-13421869 | None:intergenic |
| CATCGTCCGACTCAGGCACA+AGG | 0.584272 | 6.2:-13429139 | None:intergenic |
| TAGTGAAAGAATAGGTTGAA+GGG | 0.586040 | 6.2:-13420913 | None:intergenic |
| TCTTTCAGGGGAAGTACCAA+TGG | 0.590000 | 6.2:+13421783 | MS.gene074293:CDS |
| GGATTACTTGAACTAAGAGA+AGG | 0.592484 | 6.2:-13422523 | None:intergenic |
| CATCGTCAGACTCGGACACA+AGG | 0.605621 | 6.2:-13431274 | None:intergenic |
| GCATGTGCAGGTGGAAGTGA+AGG | 0.607259 | 6.2:+13423941 | MS.gene074293:intron |
| AGAGATGCATGTACTCAAAG+TGG | 0.616434 | 6.2:+13422493 | MS.gene074293:CDS |
| ATAACTAAGAGTTTATATGT+CGG | 0.619029 | 6.2:+13435621 | MS.gene074293:CDS |
| AGATGATTGCATTTCCAATG+TGG | 0.621973 | 6.2:-13420562 | None:intergenic |
| ACAAATTCAAACAAAGACAA+TGG | 0.623361 | 6.2:+13421900 | MS.gene074293:CDS |
| TGACAATCAAACTAAATCTG+AGG | 0.626809 | 6.2:+13422609 | MS.gene074293:CDS |
| AAGTGAAGGACAAAGAAACA+TGG | 0.633365 | 6.2:+13423955 | MS.gene074293:CDS |
| TGTGCCTGAGTCGGACGATG+AGG | 0.637272 | 6.2:+13429142 | MS.gene074293:CDS |
| GAAGCCAATAACCAATTGCA+AGG | 0.639896 | 6.2:+13434478 | MS.gene074293:CDS |
| ATTGCAAGGACTACAAGCAG+TGG | 0.647531 | 6.2:+13434492 | MS.gene074293:CDS |
| AGTGAAGGACAAAGAAACAT+GGG | 0.649441 | 6.2:+13423956 | MS.gene074293:CDS |
| TATATGCAACCCTCTTTCAG+GGG | 0.673086 | 6.2:+13421771 | MS.gene074293:CDS |
| CGTCCGACTCAGGCACAAGG+CGG | 0.678405 | 6.2:-13429136 | None:intergenic |
| TGTGTCCGAGTCTGACGATG+AGG | 0.705459 | 6.2:+13431277 | MS.gene074293:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 13420555 | 13435734 | 13420555 | ID=MS.gene074293 |
| chr6.2 | mRNA | 13420555 | 13435734 | 13420555 | ID=MS.gene074293.t1;Parent=MS.gene074293 |
| chr6.2 | exon | 13420555 | 13420786 | 13420555 | ID=MS.gene074293.t1.exon1;Parent=MS.gene074293.t1 |
| chr6.2 | CDS | 13420555 | 13420786 | 13420555 | ID=cds.MS.gene074293.t1;Parent=MS.gene074293.t1 |
| chr6.2 | exon | 13420903 | 13420947 | 13420903 | ID=MS.gene074293.t1.exon2;Parent=MS.gene074293.t1 |
| chr6.2 | CDS | 13420903 | 13420947 | 13420903 | ID=cds.MS.gene074293.t1;Parent=MS.gene074293.t1 |
| chr6.2 | exon | 13421680 | 13421976 | 13421680 | ID=MS.gene074293.t1.exon3;Parent=MS.gene074293.t1 |
| chr6.2 | CDS | 13421680 | 13421976 | 13421680 | ID=cds.MS.gene074293.t1;Parent=MS.gene074293.t1 |
| chr6.2 | exon | 13422470 | 13422636 | 13422470 | ID=MS.gene074293.t1.exon4;Parent=MS.gene074293.t1 |
| chr6.2 | CDS | 13422470 | 13422636 | 13422470 | ID=cds.MS.gene074293.t1;Parent=MS.gene074293.t1 |
| chr6.2 | exon | 13423951 | 13424032 | 13423951 | ID=MS.gene074293.t1.exon5;Parent=MS.gene074293.t1 |
| chr6.2 | CDS | 13423951 | 13424032 | 13423951 | ID=cds.MS.gene074293.t1;Parent=MS.gene074293.t1 |
| chr6.2 | exon | 13429126 | 13429212 | 13429126 | ID=MS.gene074293.t1.exon6;Parent=MS.gene074293.t1 |
| chr6.2 | CDS | 13429126 | 13429212 | 13429126 | ID=cds.MS.gene074293.t1;Parent=MS.gene074293.t1 |
| chr6.2 | exon | 13429574 | 13429702 | 13429574 | ID=MS.gene074293.t1.exon7;Parent=MS.gene074293.t1 |
| chr6.2 | CDS | 13429574 | 13429702 | 13429574 | ID=cds.MS.gene074293.t1;Parent=MS.gene074293.t1 |
| chr6.2 | exon | 13431192 | 13431316 | 13431192 | ID=MS.gene074293.t1.exon8;Parent=MS.gene074293.t1 |
| chr6.2 | CDS | 13431192 | 13431316 | 13431192 | ID=cds.MS.gene074293.t1;Parent=MS.gene074293.t1 |
| chr6.2 | exon | 13433380 | 13433484 | 13433380 | ID=MS.gene074293.t1.exon9;Parent=MS.gene074293.t1 |
| chr6.2 | CDS | 13433380 | 13433484 | 13433380 | ID=cds.MS.gene074293.t1;Parent=MS.gene074293.t1 |
| chr6.2 | exon | 13434457 | 13434573 | 13434457 | ID=MS.gene074293.t1.exon10;Parent=MS.gene074293.t1 |
| chr6.2 | CDS | 13434457 | 13434573 | 13434457 | ID=cds.MS.gene074293.t1;Parent=MS.gene074293.t1 |
| chr6.2 | exon | 13435504 | 13435734 | 13435504 | ID=MS.gene074293.t1.exon11;Parent=MS.gene074293.t1 |
| chr6.2 | CDS | 13435504 | 13435734 | 13435504 | ID=cds.MS.gene074293.t1;Parent=MS.gene074293.t1 |
| Gene Sequence |
| Protein sequence |
