AlfalfaGEDB | MS.gene074299

M. sativa cultivar XinJiangDaYe / MS.gene074299

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00197.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074299.t1	XP_024641219.1	87.3	221	24	3	1	219	1	219	7.10E-104	386.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074299.t1	O82711	48.7	226	99	7	1	219	1	216	9.9e-46	184.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074299.t1	I3SFS0	86.5	223	26	3	1	221	1	221	6.7e-104	386.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049549	MS.gene074299	0.831132	1.99E-55	-1.69E-46
MS.gene050329	MS.gene074299	0.806062	9.87E-50	-1.69E-46
MS.gene051131	MS.gene074299	0.807712	4.42E-50	-1.69E-46
MS.gene051996	MS.gene074299	0.830652	2.60E-55	-1.69E-46
MS.gene052211	MS.gene074299	0.814974	1.17E-51	-1.69E-46
MS.gene052223	MS.gene074299	0.835844	1.32E-56	-1.69E-46
MS.gene05240	MS.gene074299	0.84963	2.86E-60	-1.69E-46
MS.gene052706	MS.gene074299	0.800712	1.27E-48	-1.69E-46
MS.gene053406	MS.gene074299	0.815727	7.97E-52	-1.69E-46
MS.gene054120	MS.gene074299	0.836638	8.32E-57	-1.69E-46
MS.gene054138	MS.gene074299	0.832612	8.56E-56	-1.69E-46
MS.gene054277	MS.gene074299	0.846105	2.68E-59	-1.69E-46
MS.gene054742	MS.gene074299	0.903484	3.31E-79	-1.69E-46
MS.gene056076	MS.gene074299	0.813223	2.85E-51	-1.69E-46
MS.gene056330	MS.gene074299	0.858953	5.78E-63	-1.69E-46
MS.gene056590	MS.gene074299	0.826216	3.06E-54	-1.69E-46
MS.gene056591	MS.gene074299	0.823283	1.51E-53	-1.69E-46
MS.gene056680	MS.gene074299	0.820077	8.30E-53	-1.69E-46
MS.gene056920	MS.gene074299	0.869969	2.08E-66	-1.69E-46
MS.gene056922	MS.gene074299	0.828082	1.10E-54	-1.69E-46
MS.gene056923	MS.gene074299	0.817247	3.64E-52	-1.69E-46
MS.gene056924	MS.gene074299	0.814953	1.19E-51	-1.69E-46
MS.gene056925	MS.gene074299	0.805172	1.52E-49	-1.69E-46
MS.gene059328	MS.gene074299	0.813158	2.95E-51	-1.69E-46
MS.gene059892	MS.gene074299	0.802203	6.27E-49	-1.69E-46
MS.gene059955	MS.gene074299	0.805388	1.37E-49	-1.69E-46
MS.gene060087	MS.gene074299	0.84257	2.39E-58	-1.69E-46
MS.gene060405	MS.gene074299	0.80911	2.23E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074299.t1	MTR_6g017225	87.273	220	24	3	1	218	1	218	1.75e-138	392
MS.gene074299.t1	MTR_6g009650	50.711	211	92	6	1	208	1	202	8.74e-60	187
MS.gene074299.t1	MTR_6g066190	49.770	217	97	7	1	214	1	208	1.91e-55	176
MS.gene074299.t1	MTR_6g059730	52.000	225	92	8	1	219	1	215	2.35e-55	176
MS.gene074299.t1	MTR_6g009563	48.113	212	97	7	1	208	1	203	2.91e-54	173
MS.gene074299.t1	MTR_6g059680	53.947	228	87	7	1	219	1	219	1.27e-53	171
MS.gene074299.t1	MTR_6g066210	48.182	220	98	7	1	214	1	210	3.88e-53	170
MS.gene074299.t1	MTR_6g065460	45.192	208	99	6	7	208	6	204	1.21e-39	135
MS.gene074299.t1	MTR_1g035770	41.262	206	105	8	1	195	1	201	1.27e-36	128
MS.gene074299.t1	MTR_6g059810	42.857	210	100	10	7	204	10	211	3.31e-35	124
MS.gene074299.t1	MTR_6g059650	42.105	190	96	7	20	205	20	199	9.74e-32	115
MS.gene074299.t1	MTR_6g059530	39.819	221	110	9	1	216	1	203	4.52e-30	110
MS.gene074299.t1	MTR_6g065460	40.976	205	88	7	7	208	6	180	2.47e-29	108
MS.gene074299.t1	MTR_6g059410	40.000	210	104	8	12	216	10	202	5.60e-29	108
MS.gene074299.t1	MTR_3g463590	44.086	186	93	6	27	208	25	203	2.26e-28	106
MS.gene074299.t1	MTR_6g059760	38.636	220	105	12	1	208	1	202	7.12e-25	97.4
MS.gene074299.t1	MTR_6g045433	35.747	221	115	11	1	206	1	209	1.75e-22	91.7
MS.gene074299.t1	MTR_6g044810	44.800	125	62	5	1	119	1	124	2.58e-22	90.9
MS.gene074299.t1	MTR_6g044780	42.400	125	65	5	1	119	1	124	2.21e-21	89.0
MS.gene074299.t1	MTR_6g045097	41.538	130	69	5	1	124	1	129	8.22e-21	87.4
MS.gene074299.t1	MTR_6g045403	41.129	124	67	4	1	119	1	123	2.32e-19	83.2
MS.gene074299.t1	MTR_6g065570	55.932	59	25	1	18	75	14	72	3.88e-16	71.6
MS.gene074299.t1	MTR_2g073010	32.536	209	115	8	5	208	11	198	8.08e-16	73.2
MS.gene074299.t1	MTR_3g106585	27.922	154	107	3	9	161	7	157	4.52e-12	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 39 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCATTCCTGAAATAGTTTA+TGG	0.126621	6.2:-13181331	MS.gene074299:CDS
CTAGTCTTAGTCCACCTTTA+TGG	0.206660	6.2:+13181584	None:intergenic
AATGTGCCATAAACTATTTC+AGG	0.256857	6.2:+13181325	None:intergenic
TAGTCTTAGTCCACCTTTAT+GGG	0.257214	6.2:+13181585	None:intergenic
TATTCACCACCTGGGATAAT+AGG	0.263882	6.2:+13181634	None:intergenic
TGTGATGATTTAACACAATT+AGG	0.276879	6.2:+13181418	None:intergenic
CAAACGTGATCCACCATCTT+TGG	0.300735	6.2:+13181204	None:intergenic
AGTGAACTCAATCTTAAGTT+TGG	0.335314	6.2:+13181447	None:intergenic
TATGGAAATGCTTACAAGCT+TGG	0.389736	6.2:-13181289	MS.gene074299:CDS
TGAGAAAGCTAATGAAAGAT+TGG	0.400037	6.2:+13181690	None:intergenic
CCAGTGAAATTTAGTATACA+AGG	0.409892	6.2:-13181499	MS.gene074299:CDS
TCAACAAACACAACAGGATA+AGG	0.415700	6.2:+13181145	None:intergenic
CTCAAATGATGAGCAAGTAT+TGG	0.420196	6.2:-13181671	MS.gene074299:CDS
GATTGGTGATGAAAGCAAAT+AGG	0.420483	6.2:+13181707	None:intergenic
ATTCACCACCTGGGATAATA+GGG	0.425807	6.2:+13181635	None:intergenic
AGAATTCCAGATTCAAAAGA+AGG	0.484094	6.2:+13181118	None:intergenic
CCTTGTATACTAAATTTCAC+TGG	0.490517	6.2:+13181499	None:intergenic
ACACAACAGGATAAGGGTCT+TGG	0.505098	6.2:+13181152	None:intergenic
CAACAAACACAACAGGATAA+GGG	0.506442	6.2:+13181146	None:intergenic
GGAGCATCAACAAACACAAC+AGG	0.508650	6.2:+13181139	None:intergenic
AGTCTTAGTCCACCTTTATG+GGG	0.513810	6.2:+13181586	None:intergenic
GAGCAAGTATTGGACACAAA+TGG	0.522608	6.2:-13181661	MS.gene074299:CDS
AATAGGACACTTTGAATCAC+CGG	0.528264	6.2:+13181555	None:intergenic
TTGCAAAATGAAAATATCAC+AGG	0.530508	6.2:-13181526	MS.gene074299:CDS
AGCAATGGTATAATCTTGAC+AGG	0.537642	6.2:-13181472	MS.gene074299:CDS
GTCTTAGTCCACCTTTATGG+GGG	0.571887	6.2:+13181587	None:intergenic
AGATGGAATAGTTCCAAAGA+TGG	0.573489	6.2:-13181217	MS.gene074299:CDS
AGCAAATAGGAGGAAGCAAA+GGG	0.576992	6.2:+13181720	None:intergenic
TGGTGATGAAAGCAAATAGG+AGG	0.577210	6.2:+13181710	None:intergenic
AAAATGTAGTATTCACCACC+TGG	0.578631	6.2:+13181625	None:intergenic
ATTGTCTTGATATTATGAGA+TGG	0.582015	6.2:-13181234	MS.gene074299:CDS
AAGCAAATAGGAGGAAGCAA+AGG	0.602841	6.2:+13181719	None:intergenic
AATGGTAACCCTATTATCCC+AGG	0.611649	6.2:-13181643	MS.gene074299:CDS
TGGAATAGTTCCAAAGATGG+TGG	0.615651	6.2:-13181214	MS.gene074299:CDS
AGTATACAAGGAATAAGCAA+TGG	0.619388	6.2:-13181487	MS.gene074299:CDS
ATTGTGTTAAATCATCACAA+TGG	0.653286	6.2:-13181414	MS.gene074299:CDS
AAATGTAGTATTCACCACCT+GGG	0.670626	6.2:+13181626	None:intergenic
GGTAACCCTATTATCCCAGG+TGG	0.715318	6.2:-13181640	MS.gene074299:CDS
TTTGTAATGATGATGATACG+GGG	0.729609	6.2:-13181264	MS.gene074299:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ACATTTTATATTCAGAAATA+TGG	-	chr6.2:13181534-13181553	MS.gene074299:CDS	15.0%
!!!	CGTATTTTTAATGTTATTAT+TGG	-	chr6.2:13181474-13181493	MS.gene074299:CDS	15.0%
!!!	ATTTTGTAATGATGATGATA+CGG	-	chr6.2:13181575-13181594	MS.gene074299:CDS	20.0%
!	ATTGTCTTGATATTATGAGA+TGG	-	chr6.2:13181607-13181626	MS.gene074299:CDS	25.0%
!	ATTGTGTTAAATCATCACAA+TGG	-	chr6.2:13181427-13181446	MS.gene074299:CDS	25.0%
!	TGTGATGATTTAACACAATT+AGG	+	chr6.2:13181426-13181445	None:intergenic	25.0%
!	TTCATTCCTGAAATAGTTTA+TGG	-	chr6.2:13181510-13181529	MS.gene074299:CDS	25.0%
!	TTGCAAAATGAAAATATCAC+AGG	-	chr6.2:13181315-13181334	MS.gene074299:CDS	25.0%
!!	TCATTTTGCAAGATAGTAAT+AGG	+	chr6.2:13181306-13181325	None:intergenic	25.0%
!!!	TTTTGAATCTGGAATTCTTA+TGG	-	chr6.2:13181728-13181747	MS.gene074299:CDS	25.0%
!!!	TTTTGTAATGATGATGATAC+GGG	-	chr6.2:13181576-13181595	MS.gene074299:CDS	25.0%
	AATGTGCCATAAACTATTTC+AGG	+	chr6.2:13181519-13181538	None:intergenic	30.0%
	AGAATTCCAGATTCAAAAGA+AGG	+	chr6.2:13181726-13181745	None:intergenic	30.0%
	AGTATACAAGGAATAAGCAA+TGG	-	chr6.2:13181354-13181373	MS.gene074299:CDS	30.0%
	AGTGAACTCAATCTTAAGTT+TGG	+	chr6.2:13181397-13181416	None:intergenic	30.0%
	CCAGTGAAATTTAGTATACA+AGG	-	chr6.2:13181342-13181361	MS.gene074299:CDS	30.0%
	CCTTGTATACTAAATTTCAC+TGG	+	chr6.2:13181345-13181364	None:intergenic	30.0%
	TGAGAAAGCTAATGAAAGAT+TGG	+	chr6.2:13181154-13181173	None:intergenic	30.0%
!	TTTGTAATGATGATGATACG+GGG	-	chr6.2:13181577-13181596	MS.gene074299:CDS	30.0%
	AAAATGTAGTATTCACCACC+TGG	+	chr6.2:13181219-13181238	None:intergenic	35.0%
	AAATGTAGTATTCACCACCT+GGG	+	chr6.2:13181218-13181237	None:intergenic	35.0%
	AATAGGACACTTTGAATCAC+CGG	+	chr6.2:13181289-13181308	None:intergenic	35.0%
	AGATGGAATAGTTCCAAAGA+TGG	-	chr6.2:13181624-13181643	MS.gene074299:CDS	35.0%
	AGCAATGGTATAATCTTGAC+AGG	-	chr6.2:13181369-13181388	MS.gene074299:CDS	35.0%
	CAACAAACACAACAGGATAA+GGG	+	chr6.2:13181698-13181717	None:intergenic	35.0%
	CTCAAATGATGAGCAAGTAT+TGG	-	chr6.2:13181170-13181189	MS.gene074299:CDS	35.0%
	TATGGAAATGCTTACAAGCT+TGG	-	chr6.2:13181552-13181571	MS.gene074299:CDS	35.0%
	TCAACAAACACAACAGGATA+AGG	+	chr6.2:13181699-13181718	None:intergenic	35.0%
!	TAGTCTTAGTCCACCTTTAT+GGG	+	chr6.2:13181259-13181278	None:intergenic	35.0%
!!	GATTGGTGATGAAAGCAAAT+AGG	+	chr6.2:13181137-13181156	None:intergenic	35.0%
	AAGCAAATAGGAGGAAGCAA+AGG	+	chr6.2:13181125-13181144	None:intergenic	40.0%
	AATGGTAACCCTATTATCCC+AGG	-	chr6.2:13181198-13181217	MS.gene074299:CDS	40.0%
	AGCAAATAGGAGGAAGCAAA+GGG	+	chr6.2:13181124-13181143	None:intergenic	40.0%
	AGTCTTAGTCCACCTTTATG+GGG	+	chr6.2:13181258-13181277	None:intergenic	40.0%
	ATTCACCACCTGGGATAATA+GGG	+	chr6.2:13181209-13181228	None:intergenic	40.0%
	GGACTAAGACTAGCAAAAAC+CGG	-	chr6.2:13181267-13181286	MS.gene074299:CDS	40.0%
	TATTCACCACCTGGGATAAT+AGG	+	chr6.2:13181210-13181229	None:intergenic	40.0%
	TGGAATAGTTCCAAAGATGG+TGG	-	chr6.2:13181627-13181646	MS.gene074299:CDS	40.0%
!	GATGCTCCTTCTTTTGAATC+TGG	-	chr6.2:13181717-13181736	MS.gene074299:CDS	40.0%
!	TGGTGATGAAAGCAAATAGG+AGG	+	chr6.2:13181134-13181153	None:intergenic	40.0%
!!	CTAGTCTTAGTCCACCTTTA+TGG	+	chr6.2:13181260-13181279	None:intergenic	40.0%
!!	GAGCAAGTATTGGACACAAA+TGG	-	chr6.2:13181180-13181199	MS.gene074299:CDS	40.0%
	ACACAACAGGATAAGGGTCT+TGG	+	chr6.2:13181692-13181711	None:intergenic	45.0%
	CAAACGTGATCCACCATCTT+TGG	+	chr6.2:13181640-13181659	None:intergenic	45.0%
	GTCTTAGTCCACCTTTATGG+GGG	+	chr6.2:13181257-13181276	None:intergenic	45.0%
!	GGAGCATCAACAAACACAAC+AGG	+	chr6.2:13181705-13181724	None:intergenic	45.0%
	ACTCAAGACCCCCATAAAGG+TGG	-	chr6.2:13181246-13181265	MS.gene074299:CDS	50.0%
	GCAACTCAAGACCCCCATAA+AGG	-	chr6.2:13181243-13181262	MS.gene074299:CDS	50.0%
	GGTAACCCTATTATCCCAGG+TGG	-	chr6.2:13181201-13181220	MS.gene074299:CDS	50.0%
!	TTATGGGGGTCTTGAGTTGC+TGG	+	chr6.2:13181243-13181262	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	13181099	13181764	13181099	ID=MS.gene074299
chr6.2	mRNA	13181099	13181764	13181099	ID=MS.gene074299.t1;Parent=MS.gene074299
chr6.2	exon	13181099	13181764	13181099	ID=MS.gene074299.t1.exon1;Parent=MS.gene074299.t1
chr6.2	CDS	13181099	13181764	13181099	ID=cds.MS.gene074299.t1;Parent=MS.gene074299.t1

Gene Sequence

>MS.gene074299

ATGAAGCTTGTTTTATCACTCACCCTTTGCTTCCTCCTATTTGCTTTCATCACCAATCTTTCATTAGCTTTCTCAAATGATGAGCAAGTATTGGACACAAATGGTAACCCTATTATCCCAGGTGGTGAATACTACATTTTTCCAGCAACTCAAGACCCCCATAAAGGTGGACTAAGACTAGCAAAAACCGGTGATTCAAAGTGTCCTATTACTATCTTGCAAAATGAAAATATCACAGGTTTACCAGTGAAATTTAGTATACAAGGAATAAGCAATGGTATAATCTTGACAGGTACCAAACTTAAGATTGAGTTCACTAAGAAGCCTAATTGTGTTAAATCATCACAATGGTTGATGTTTTTAGATGATGATGCTCGTATTTTTAATGTTATTATTGGTGCTTCTGATGTATTCATTCCTGAAATAGTTTATGGCACATTTTATATTCAGAAATATGGAAATGCTTACAAGCTTGGATTTTGTAATGATGATGATACGGGGAAACAAAATTGTCTTGATATTATGAGATGGAATAGTTCCAAAGATGGTGGATCACGTTTGAGATTGATTTTGAAAGCTGATCAAGAAGTCCAAGACCCTTATCCTGTTGTGTTTGTTGATGCTCCTTCTTTTGAATCTGGAATTCTTATGGATGTGAGACTTTGA

Protein sequence

>MS.gene074299.t1

MKLVLSLTLCFLLFAFITNLSLAFSNDEQVLDTNGNPIIPGGEYYIFPATQDPHKGGLRLAKTGDSKCPITILQNENITGLPVKFSIQGISNGIILTGTKLKIEFTKKPNCVKSSQWLMFLDDDARIFNVIIGASDVFIPEIVYGTFYIQKYGNAYKLGFCNDDDTGKQNCLDIMRWNSSKDGGSRLRLILKADQEVQDPYPVVFVDAPSFESGILMDVRL