AlfalfaGEDB | MS.gene08594

M. sativa cultivar XinJiangDaYe / MS.gene08594

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08594.t1	XP_003606464.1	97.9	94	2	0	1	94	1	94	7.30E-42	179.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08594.t1	G7JQH1	97.9	94	2	0	1	94	1	94	5.3e-42	179.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050020	MS.gene08594	0.816183	6.31E-52	-1.69E-46
MS.gene050169	MS.gene08594	-0.804822	1.80E-49	-1.69E-46
MS.gene050546	MS.gene08594	0.802507	5.43E-49	-1.69E-46
MS.gene050787	MS.gene08594	0.807778	4.28E-50	-1.69E-46
MS.gene051265	MS.gene08594	0.808463	3.06E-50	-1.69E-46
MS.gene052190	MS.gene08594	0.825968	3.51E-54	-1.69E-46
MS.gene053054	MS.gene08594	-0.841963	3.45E-58	-1.69E-46
MS.gene053689	MS.gene08594	0.800749	1.25E-48	-1.69E-46
MS.gene054291	MS.gene08594	-0.812318	4.50E-51	-1.69E-46
MS.gene054433	MS.gene08594	0.834258	3.33E-56	-1.69E-46
MS.gene054467	MS.gene08594	0.800111	1.68E-48	-1.69E-46
MS.gene054468	MS.gene08594	0.803705	3.07E-49	-1.69E-46
MS.gene055356	MS.gene08594	0.855229	7.26E-62	-1.69E-46
MS.gene055357	MS.gene08594	0.842666	2.25E-58	-1.69E-46
MS.gene055358	MS.gene08594	0.868577	5.89E-66	-1.69E-46
MS.gene055507	MS.gene08594	0.812856	3.43E-51	-1.69E-46
MS.gene057060	MS.gene08594	0.810885	9.23E-51	-1.69E-46
MS.gene059607	MS.gene08594	0.841151	5.66E-58	-1.69E-46
MS.gene060137	MS.gene08594	-0.817559	3.10E-52	-1.69E-46
MS.gene061114	MS.gene08594	-0.804079	2.57E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08594.t1	MTR_4g060620	97.872	94	2	0	1	94	1	94	8.00e-65	190

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08594.t1	AT5G60400	40.449	89	51	1	6	94	5	91	8.94e-12	56.2

Find 13 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGTTCCTCTCTGTCTTTC+TGG	0.075036	4.1:+38523557	MS.gene08594:CDS
TGATGATGATCTTGAAGATC+TGG	0.442771	4.1:+38523852	MS.gene08594:CDS
AGTGCATGCAAATCAAGAAA+AGG	0.472454	4.1:+38523786	MS.gene08594:CDS
TTAAACCTTCGTGCTACTCA+TGG	0.498021	4.1:+38523536	MS.gene08594:CDS
ACCGAAGAAGAAAAGATATG+AGG	0.524381	4.1:+38523752	MS.gene08594:CDS
GTGCATGCAAATCAAGAAAA+GGG	0.524986	4.1:+38523787	MS.gene08594:CDS
CTGTCTCTTACTTATCTCAC+AGG	0.529035	4.1:+38523892	MS.gene08594:CDS
TACTCTTTGGAAAGAGCAAG+AGG	0.575505	4.1:-38523590	None:intergenic
TTTGGAAAGAGCAAGAGGGT+TGG	0.601098	4.1:-38523585	None:intergenic
ACTCTTTGGAAAGAGCAAGA+GGG	0.667434	4.1:-38523589	None:intergenic
AGTAGCACGAAGGTTTAACA+TGG	0.672060	4.1:-38523531	None:intergenic
GTGATTCCAGAAAGACAGAG+AGG	0.712435	4.1:-38523563	None:intergenic
GAACTCCATGAGTAGCACGA+AGG	0.732005	4.1:-38523541	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATAAATTAATTTACTCTT+TGG	-	chr4.1:38523606-38523625	None:intergenic	10.0%
!!	TGATATATGTTTGAATTTGA+TGG	+	chr4.1:38523710-38523729	MS.gene08594:intron	20.0%
!!	TCAAAGAAACAATGATTTTG+TGG	+	chr4.1:38523819-38523838	MS.gene08594:CDS	25.0%
!	AATGATTTTGTGGAGATGAA+AGG	+	chr4.1:38523829-38523848	MS.gene08594:CDS	30.0%
!	TCCTCATATCTTTTCTTCTT+CGG	-	chr4.1:38523756-38523775	None:intergenic	30.0%
	ACCGAAGAAGAAAAGATATG+AGG	+	chr4.1:38523752-38523771	MS.gene08594:CDS	35.0%
	AGTGCATGCAAATCAAGAAA+AGG	+	chr4.1:38523786-38523805	MS.gene08594:CDS	35.0%
	GTGAGATCATAGATCAAAAG+AGG	-	chr4.1:38523648-38523667	None:intergenic	35.0%
	GTGCATGCAAATCAAGAAAA+GGG	+	chr4.1:38523787-38523806	MS.gene08594:CDS	35.0%
!	TGATGATGATCTTGAAGATC+TGG	+	chr4.1:38523852-38523871	MS.gene08594:CDS	35.0%
!!	ATGTTTGAATTTGATGGTGC+AGG	+	chr4.1:38523716-38523735	MS.gene08594:intron	35.0%
	AGTAGCACGAAGGTTTAACA+TGG	-	chr4.1:38523534-38523553	None:intergenic	40.0%
	CTGTCTCTTACTTATCTCAC+AGG	+	chr4.1:38523892-38523911	MS.gene08594:CDS	40.0%
!	ACTCTTTGGAAAGAGCAAGA+GGG	-	chr4.1:38523592-38523611	None:intergenic	40.0%
!	TACTCTTTGGAAAGAGCAAG+AGG	-	chr4.1:38523593-38523612	None:intergenic	40.0%
!	TTAAACCTTCGTGCTACTCA+TGG	+	chr4.1:38523536-38523555	MS.gene08594:CDS	40.0%
!!	TTGAATTTGATGGTGCAGGA+TGG	+	chr4.1:38523720-38523739	MS.gene08594:intron	40.0%
	GTGATTCCAGAAAGACAGAG+AGG	-	chr4.1:38523566-38523585	None:intergenic	45.0%
	TTTGGAAAGAGCAAGAGGGT+TGG	-	chr4.1:38523588-38523607	None:intergenic	45.0%
	GAACTCCATGAGTAGCACGA+AGG	-	chr4.1:38523544-38523563	None:intergenic	50.0%
	GGAGTTCCTCTCTGTCTTTC+TGG	+	chr4.1:38523557-38523576	MS.gene08594:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	38523524	38523936	38523524	ID=MS.gene08594
chr4.1	mRNA	38523524	38523936	38523524	ID=MS.gene08594.t1;Parent=MS.gene08594
chr4.1	exon	38523524	38523609	38523524	ID=MS.gene08594.t1.exon1;Parent=MS.gene08594.t1
chr4.1	CDS	38523524	38523609	38523524	ID=cds.MS.gene08594.t1;Parent=MS.gene08594.t1
chr4.1	exon	38523738	38523936	38523738	ID=MS.gene08594.t1.exon2;Parent=MS.gene08594.t1
chr4.1	CDS	38523738	38523936	38523738	ID=cds.MS.gene08594.t1;Parent=MS.gene08594.t1

Gene Sequence

>MS.gene08594

ATGCAGTCCATGTTAAACCTTCGTGCTACTCATGGAGTTCCTCTCTGTCTTTCTGGAATCACCAACCCTCTTGCTCTTTCCAAAGAGTAAATTAATTTATTTATTAATTAATTAATTAACACCTCTTTTGATCTATGATCTCACTTGTTTTTTTTTTTTTTTTTTGATAATTATATTCTCTTATTTTGATATATGTTTGAATTTGATGGTGCAGGATGGCAGATCACGACCGAAGAAGAAAAGATATGAGGAAGCAAAGATCAGTGCATGCAAATCAAGAAAAGGGTGCACAAAGTCAAAGAAACAATGATTTTGTGGAGATGAAAGGTGATGATGATCTTGAAGATCTGGATGAAATCTTGCATCAACTGTCTCTTACTTATCTCACAGGTTTATCAGCAAAAGCTGCTTAG

Protein sequence

>MS.gene08594.t1

MQSMLNLRATHGVPLCLSGITNPLALSKEMADHDRRRKDMRKQRSVHANQEKGAQSQRNNDFVEMKGDDDLEDLDEILHQLSLTYLTGLSAKAA