Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24380.t1 | XP_003589223.1 | 95.2 | 208 | 10 | 0 | 1 | 208 | 1 | 208 | 5.60E-111 | 410.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24380.t1 | G7I4F7 | 95.2 | 208 | 10 | 0 | 1 | 208 | 1 | 208 | 4.1e-111 | 410.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050442 | MS.gene24380 | 0.816483 | 5.40E-52 | -1.69E-46 |
| MS.gene050680 | MS.gene24380 | 0.803301 | 3.72E-49 | -1.69E-46 |
| MS.gene050750 | MS.gene24380 | 0.803321 | 3.69E-49 | -1.69E-46 |
| MS.gene050787 | MS.gene24380 | 0.832648 | 8.38E-56 | -1.69E-46 |
| MS.gene050844 | MS.gene24380 | 0.813239 | 2.83E-51 | -1.69E-46 |
| MS.gene051207 | MS.gene24380 | 0.815803 | 7.67E-52 | -1.69E-46 |
| MS.gene051265 | MS.gene24380 | 0.842482 | 2.52E-58 | -1.69E-46 |
| MS.gene052190 | MS.gene24380 | 0.812645 | 3.82E-51 | -1.69E-46 |
| MS.gene052333 | MS.gene24380 | 0.80638 | 8.46E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene24380 | 0.80048 | 1.41E-48 | -1.69E-46 |
| MS.gene053166 | MS.gene24380 | 0.833167 | 6.23E-56 | -1.69E-46 |
| MS.gene054093 | MS.gene24380 | 0.802348 | 5.86E-49 | -1.69E-46 |
| MS.gene054236 | MS.gene24380 | 0.80195 | 7.07E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene24380 | -0.815085 | 1.11E-51 | -1.69E-46 |
| MS.gene054467 | MS.gene24380 | 0.845547 | 3.79E-59 | -1.69E-46 |
| MS.gene054468 | MS.gene24380 | 0.842691 | 2.22E-58 | -1.69E-46 |
| MS.gene054472 | MS.gene24380 | 0.800008 | 1.76E-48 | -1.69E-46 |
| MS.gene054559 | MS.gene24380 | 0.824199 | 9.19E-54 | -1.69E-46 |
| MS.gene05468 | MS.gene24380 | 0.82204 | 2.93E-53 | -1.69E-46 |
| MS.gene055356 | MS.gene24380 | 0.853877 | 1.79E-61 | -1.69E-46 |
| MS.gene055357 | MS.gene24380 | 0.830547 | 2.76E-55 | -1.69E-46 |
| MS.gene055358 | MS.gene24380 | 0.858032 | 1.09E-62 | -1.69E-46 |
| MS.gene055701 | MS.gene24380 | 0.812742 | 3.64E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene24380 | 0.813435 | 2.56E-51 | -1.69E-46 |
| MS.gene057026 | MS.gene24380 | 0.806654 | 7.41E-50 | -1.69E-46 |
| MS.gene059128 | MS.gene24380 | 0.802053 | 6.73E-49 | -1.69E-46 |
| MS.gene059272 | MS.gene24380 | 0.800196 | 1.61E-48 | -1.69E-46 |
| MS.gene059609 | MS.gene24380 | 0.808086 | 3.68E-50 | -1.69E-46 |
| MS.gene060137 | MS.gene24380 | -0.810095 | 1.37E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene24380 | 0.809363 | 1.96E-50 | -1.69E-46 |
| MS.gene060683 | MS.gene24380 | 0.814566 | 1.44E-51 | -1.69E-46 |
| MS.gene060685 | MS.gene24380 | 0.820625 | 6.21E-53 | -1.69E-46 |
| MS.gene061114 | MS.gene24380 | -0.806872 | 6.66E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24380.t1 | MTR_1g019800 | 95.192 | 208 | 10 | 0 | 1 | 208 | 1 | 208 | 7.13e-148 | 409 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24380.t1 | AT1G47410 | 38.500 | 200 | 87 | 6 | 21 | 208 | 9 | 184 | 1.98e-32 | 116 |
| MS.gene24380.t1 | AT1G47410 | 38.500 | 200 | 87 | 6 | 21 | 208 | 9 | 184 | 1.98e-32 | 116 |
Find 41 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCATCAAGATCACTAAT+TGG | 0.271099 | 1.1:+3343690 | None:intergenic |
| ATTATGTGTTACTGGGAAAA+TGG | 0.272982 | 1.1:-3343401 | MS.gene24380:CDS |
| TTGGAAGCGTTCTATAATTC+AGG | 0.285690 | 1.1:-3343206 | MS.gene24380:CDS |
| ACAAGAGAATTATGTGTTAC+TGG | 0.321124 | 1.1:-3343409 | MS.gene24380:CDS |
| TTACCTCCCTCCTTTGCCTT+TGG | 0.326286 | 1.1:+3343376 | None:intergenic |
| GTGTATTCTTCTAGTGCTAA+TGG | 0.339241 | 1.1:-3343301 | MS.gene24380:CDS |
| TGCAAAGCATTTGCATCTCT+TGG | 0.342800 | 1.1:+3343797 | None:intergenic |
| CATTGTGAAGGATGGCCTTT+CGG | 0.363378 | 1.1:-3343175 | MS.gene24380:CDS |
| ACACATAATTCTCTTGTTGC+AGG | 0.374763 | 1.1:+3343415 | None:intergenic |
| TGTATTCTTCTAGTGCTAAT+GGG | 0.374850 | 1.1:-3343300 | MS.gene24380:CDS |
| TTGCATCTCTTGGAATGGTT+TGG | 0.387372 | 1.1:+3343807 | None:intergenic |
| CATATAAATTCAAGTAACTT+TGG | 0.429792 | 1.1:+3343069 | None:intergenic |
| TAGTGATCTTGATGAAGAAC+AGG | 0.434961 | 1.1:-3343685 | MS.gene24380:CDS |
| ATTCAAGTAACTTTGGTACT+TGG | 0.457278 | 1.1:+3343076 | None:intergenic |
| ATGCTTTGCAGCTGCTCTGA+AGG | 0.476673 | 1.1:-3343784 | MS.gene24380:CDS |
| TAATTGGAAACTCCTCTTCA+AGG | 0.478540 | 1.1:+3343706 | None:intergenic |
| GGTTTGGTTGCTCCTCCTCA+TGG | 0.490726 | 1.1:+3343823 | None:intergenic |
| GGCGGTTAGAAGCCGAGCTA+TGG | 0.496315 | 1.1:-3343494 | MS.gene24380:CDS |
| AAACTCTCTCTCAGTAGGCT+TGG | 0.513974 | 1.1:+3343454 | None:intergenic |
| CTAAGTCACCATCTGAATCA+TGG | 0.518553 | 1.1:-3343117 | MS.gene24380:CDS |
| AGCATTTGCATCTCTTGGAA+TGG | 0.526952 | 1.1:+3343802 | None:intergenic |
| CAAGAGAATTATGTGTTACT+GGG | 0.534810 | 1.1:-3343408 | MS.gene24380:CDS |
| TTCGACCAAAAGTTTGACAC+TGG | 0.545482 | 1.1:+3343751 | None:intergenic |
| TCTTCTAGTGCTAATGGGGA+AGG | 0.547226 | 1.1:-3343295 | MS.gene24380:CDS |
| TCAAGCTTGAACATCTTGAA+AGG | 0.551732 | 1.1:+3343251 | None:intergenic |
| AATGGAACCAAAGGCAAAGG+AGG | 0.558037 | 1.1:-3343383 | MS.gene24380:CDS |
| TACTGGGAAAATGGAACCAA+AGG | 0.569555 | 1.1:-3343392 | MS.gene24380:CDS |
| TTCGACTACAAGCCTTGAAG+AGG | 0.572674 | 1.1:-3343718 | MS.gene24380:CDS |
| CGATACAACCATGATTCAGA+TGG | 0.575454 | 1.1:+3343109 | None:intergenic |
| CATGATTCAGATGGTGACTT+AGG | 0.584417 | 1.1:+3343118 | None:intergenic |
| TCGACCAAAAGTTTGACACT+GGG | 0.608137 | 1.1:+3343752 | None:intergenic |
| GTATTCTTCTAGTGCTAATG+GGG | 0.608962 | 1.1:-3343299 | MS.gene24380:CDS |
| GATGGTGACTTAGGCAGTGG+TGG | 0.623202 | 1.1:+3343127 | None:intergenic |
| GAAAATGGAACCAAAGGCAA+AGG | 0.636908 | 1.1:-3343386 | MS.gene24380:CDS |
| ATGGAACCAAAGGCAAAGGA+GGG | 0.641016 | 1.1:-3343382 | MS.gene24380:CDS |
| GCTTTACGACAGAGACCGAA+AGG | 0.645910 | 1.1:+3343160 | None:intergenic |
| GAGGGAGGTAATGAAGAACA+AGG | 0.652290 | 1.1:-3343364 | MS.gene24380:CDS |
| TCAGATGGTGACTTAGGCAG+TGG | 0.663320 | 1.1:+3343124 | None:intergenic |
| GAACCAAAGGCAAAGGAGGG+AGG | 0.663364 | 1.1:-3343379 | MS.gene24380:CDS |
| TCTTCAGGTGATTGTCTCGG+CGG | 0.664433 | 1.1:-3343512 | MS.gene24380:intron |
| TGTTGCAGGAGAATATACCC+AGG | 0.714302 | 1.1:+3343429 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCAAAAATAGTTTGATTTTA+AGG | + | chr1.1:3343287-3343306 | None:intergenic | 15.0% |
| !!! | ATTTTGATTTGTCAGAATAT+TGG | - | chr1.1:3343690-3343709 | MS.gene24380:CDS | 20.0% |
| !! | AATTAGTTATCGTTCTTTTG+TGG | - | chr1.1:3343377-3343396 | MS.gene24380:CDS | 25.0% |
| !!! | TAGTTTGATTTTAAGGTTGA+AGG | + | chr1.1:3343280-3343299 | None:intergenic | 25.0% |
| ACAAGAGAATTATGTGTTAC+TGG | - | chr1.1:3343506-3343525 | MS.gene24380:CDS | 30.0% | |
| TCTTCATCAAGATCACTAAT+TGG | + | chr1.1:3343228-3343247 | None:intergenic | 30.0% | |
| TGTATTCTTCTAGTGCTAAT+GGG | - | chr1.1:3343615-3343634 | MS.gene24380:intron | 30.0% | |
| ! | ATTATGTGTTACTGGGAAAA+TGG | - | chr1.1:3343514-3343533 | MS.gene24380:intron | 30.0% |
| ! | CAAGAGAATTATGTGTTACT+GGG | - | chr1.1:3343507-3343526 | MS.gene24380:CDS | 30.0% |
| ! | TGAAAGAGAAGAGTTTTTGT+CGG | - | chr1.1:3343574-3343593 | MS.gene24380:intron | 30.0% |
| ACACATAATTCTCTTGTTGC+AGG | + | chr1.1:3343503-3343522 | None:intergenic | 35.0% | |
| GTATTCTTCTAGTGCTAATG+GGG | - | chr1.1:3343616-3343635 | MS.gene24380:intron | 35.0% | |
| GTGTATTCTTCTAGTGCTAA+TGG | - | chr1.1:3343614-3343633 | MS.gene24380:intron | 35.0% | |
| TAATTGGAAACTCCTCTTCA+AGG | + | chr1.1:3343212-3343231 | None:intergenic | 35.0% | |
| TAGTGATCTTGATGAAGAAC+AGG | - | chr1.1:3343230-3343249 | MS.gene24380:CDS | 35.0% | |
| TCAAGCTTGAACATCTTGAA+AGG | + | chr1.1:3343667-3343686 | None:intergenic | 35.0% | |
| ! | GTTCTTTTGTGGTTTTCTTC+AGG | - | chr1.1:3343388-3343407 | MS.gene24380:CDS | 35.0% |
| ! | TTTTCCCAGTGTCAAACTTT+TGG | - | chr1.1:3343159-3343178 | MS.gene24380:CDS | 35.0% |
| ! | TTTTCTTCAGGTGATTGTCT+CGG | - | chr1.1:3343400-3343419 | MS.gene24380:CDS | 35.0% |
| !! | AGTTTTGTCATTGTGAAGGA+TGG | - | chr1.1:3343732-3343751 | MS.gene24380:CDS | 35.0% |
| !! | TCTTTTGTTTCTCCATAGCT+CGG | + | chr1.1:3343436-3343455 | None:intergenic | 35.0% |
| !! | TTGGAAGCGTTCTATAATTC+AGG | - | chr1.1:3343709-3343728 | MS.gene24380:CDS | 35.0% |
| AGCATTTGCATCTCTTGGAA+TGG | + | chr1.1:3343116-3343135 | None:intergenic | 40.0% | |
| AGGAAAAACTCTCTCTCAGT+AGG | + | chr1.1:3343469-3343488 | None:intergenic | 40.0% | |
| CATGATTCAGATGGTGACTT+AGG | + | chr1.1:3343800-3343819 | None:intergenic | 40.0% | |
| CGATACAACCATGATTCAGA+TGG | + | chr1.1:3343809-3343828 | None:intergenic | 40.0% | |
| CTAAGTCACCATCTGAATCA+TGG | - | chr1.1:3343798-3343817 | MS.gene24380:CDS | 40.0% | |
| GAAAATGGAACCAAAGGCAA+AGG | - | chr1.1:3343529-3343548 | MS.gene24380:intron | 40.0% | |
| TACTGGGAAAATGGAACCAA+AGG | - | chr1.1:3343523-3343542 | MS.gene24380:intron | 40.0% | |
| TCGACCAAAAGTTTGACACT+GGG | + | chr1.1:3343166-3343185 | None:intergenic | 40.0% | |
| TTCGACCAAAAGTTTGACAC+TGG | + | chr1.1:3343167-3343186 | None:intergenic | 40.0% | |
| TTGCATCTCTTGGAATGGTT+TGG | + | chr1.1:3343111-3343130 | None:intergenic | 40.0% | |
| ! | AAACTTTTGGTCGAAGTCGA+AGG | - | chr1.1:3343172-3343191 | MS.gene24380:CDS | 40.0% |
| ! | ACTGAGAGAGAGTTTTTCCT+GGG | - | chr1.1:3343469-3343488 | MS.gene24380:CDS | 40.0% |
| ! | TACTGAGAGAGAGTTTTTCC+TGG | - | chr1.1:3343468-3343487 | MS.gene24380:CDS | 40.0% |
| ! | TGCAAAGCATTTGCATCTCT+TGG | + | chr1.1:3343121-3343140 | None:intergenic | 40.0% |
| !! | CAGGAGTTTTGTCATTGTGA+AGG | - | chr1.1:3343728-3343747 | MS.gene24380:CDS | 40.0% |
| AAACTCTCTCTCAGTAGGCT+TGG | + | chr1.1:3343464-3343483 | None:intergenic | 45.0% | |
| AATGGAACCAAAGGCAAAGG+AGG | - | chr1.1:3343532-3343551 | MS.gene24380:intron | 45.0% | |
| ATGGAACCAAAGGCAAAGGA+GGG | - | chr1.1:3343533-3343552 | MS.gene24380:intron | 45.0% | |
| CATTGTGAAGGATGGCCTTT+CGG | - | chr1.1:3343740-3343759 | MS.gene24380:CDS | 45.0% | |
| GAGGGAGGTAATGAAGAACA+AGG | - | chr1.1:3343551-3343570 | MS.gene24380:intron | 45.0% | |
| TCTTCTAGTGCTAATGGGGA+AGG | - | chr1.1:3343620-3343639 | MS.gene24380:intron | 45.0% | |
| TGTTGCAGGAGAATATACCC+AGG | + | chr1.1:3343489-3343508 | None:intergenic | 45.0% | |
| TTCGACTACAAGCCTTGAAG+AGG | - | chr1.1:3343197-3343216 | MS.gene24380:CDS | 45.0% | |
| ATGCTTTGCAGCTGCTCTGA+AGG | - | chr1.1:3343131-3343150 | MS.gene24380:CDS | 50.0% | |
| GCTTTACGACAGAGACCGAA+AGG | + | chr1.1:3343758-3343777 | None:intergenic | 50.0% | |
| TCAGATGGTGACTTAGGCAG+TGG | + | chr1.1:3343794-3343813 | None:intergenic | 50.0% | |
| TCTTCAGGTGATTGTCTCGG+CGG | - | chr1.1:3343403-3343422 | MS.gene24380:CDS | 50.0% | |
| TTACCTCCCTCCTTTGCCTT+TGG | + | chr1.1:3343542-3343561 | None:intergenic | 50.0% | |
| ! | GGGTGTTTTCTACCATGAGG+AGG | - | chr1.1:3343080-3343099 | MS.gene24380:CDS | 50.0% |
| GAACCAAAGGCAAAGGAGGG+AGG | - | chr1.1:3343536-3343555 | MS.gene24380:intron | 55.0% | |
| GATGGTGACTTAGGCAGTGG+TGG | + | chr1.1:3343791-3343810 | None:intergenic | 55.0% | |
| ! | GGTTTGGTTGCTCCTCCTCA+TGG | + | chr1.1:3343095-3343114 | None:intergenic | 55.0% |
| GGCGGTTAGAAGCCGAGCTA+TGG | - | chr1.1:3343421-3343440 | MS.gene24380:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 3343078 | 3343859 | 3343078 | ID=MS.gene24380 |
| chr1.1 | mRNA | 3343078 | 3343859 | 3343078 | ID=MS.gene24380.t1;Parent=MS.gene24380 |
| chr1.1 | exon | 3343683 | 3343859 | 3343683 | ID=MS.gene24380.t1.exon1;Parent=MS.gene24380.t1 |
| chr1.1 | CDS | 3343683 | 3343859 | 3343683 | ID=cds.MS.gene24380.t1;Parent=MS.gene24380.t1 |
| chr1.1 | exon | 3343078 | 3343527 | 3343078 | ID=MS.gene24380.t1.exon2;Parent=MS.gene24380.t1 |
| chr1.1 | CDS | 3343078 | 3343527 | 3343078 | ID=cds.MS.gene24380.t1;Parent=MS.gene24380.t1 |
| Gene Sequence |
| Protein sequence |
