Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27530.t1 | XP_003623667.1 | 92.9 | 211 | 15 | 0 | 1 | 211 | 1 | 211 | 1.10E-90 | 342.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27530.t1 | G7KZ69 | 92.9 | 211 | 15 | 0 | 1 | 211 | 1 | 211 | 8.1e-91 | 342.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene27530 | 0.866236 | 3.31E-65 | -1.69E-46 |
| MS.gene049758 | MS.gene27530 | 0.803914 | 2.78E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene27530 | 0.849067 | 4.10E-60 | -1.69E-46 |
| MS.gene050227 | MS.gene27530 | 0.843339 | 1.49E-58 | -1.69E-46 |
| MS.gene050228 | MS.gene27530 | 0.85168 | 7.58E-61 | -1.69E-46 |
| MS.gene050623 | MS.gene27530 | -0.836835 | 7.41E-57 | -1.69E-46 |
| MS.gene050642 | MS.gene27530 | 0.82195 | 3.07E-53 | -1.69E-46 |
| MS.gene050762 | MS.gene27530 | 0.828146 | 1.06E-54 | -1.69E-46 |
| MS.gene050763 | MS.gene27530 | 0.827627 | 1.41E-54 | -1.69E-46 |
| MS.gene050987 | MS.gene27530 | 0.802094 | 6.60E-49 | -1.69E-46 |
| MS.gene051535 | MS.gene27530 | 0.808911 | 2.45E-50 | -1.69E-46 |
| MS.gene051983 | MS.gene27530 | 0.851175 | 1.05E-60 | -1.69E-46 |
| MS.gene052077 | MS.gene27530 | 0.82101 | 5.07E-53 | -1.69E-46 |
| MS.gene052176 | MS.gene27530 | 0.822787 | 1.97E-53 | -1.69E-46 |
| MS.gene052309 | MS.gene27530 | -0.802034 | 6.79E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene27530 | 0.801899 | 7.24E-49 | -1.69E-46 |
| MS.gene052389 | MS.gene27530 | 0.821646 | 3.61E-53 | -1.69E-46 |
| MS.gene053073 | MS.gene27530 | 0.825532 | 4.45E-54 | -1.69E-46 |
| MS.gene053577 | MS.gene27530 | 0.805235 | 1.47E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene27530 | 0.835908 | 1.28E-56 | -1.69E-46 |
| MS.gene054379 | MS.gene27530 | 0.833506 | 5.13E-56 | -1.69E-46 |
| MS.gene054930 | MS.gene27530 | 0.875143 | 3.89E-68 | -1.69E-46 |
| MS.gene055701 | MS.gene27530 | 0.830037 | 3.68E-55 | -1.69E-46 |
| MS.gene056979 | MS.gene27530 | 0.85117 | 1.06E-60 | -1.69E-46 |
| MS.gene05761 | MS.gene27530 | 0.827251 | 1.73E-54 | -1.69E-46 |
| MS.gene058142 | MS.gene27530 | 0.826855 | 2.16E-54 | -1.69E-46 |
| MS.gene058435 | MS.gene27530 | 0.801785 | 7.64E-49 | -1.69E-46 |
| MS.gene058710 | MS.gene27530 | 0.801966 | 7.02E-49 | -1.69E-46 |
| MS.gene059128 | MS.gene27530 | 0.813402 | 2.61E-51 | -1.69E-46 |
| MS.gene059161 | MS.gene27530 | 0.80797 | 3.90E-50 | -1.69E-46 |
| MS.gene059285 | MS.gene27530 | 0.803995 | 2.67E-49 | -1.69E-46 |
| MS.gene059899 | MS.gene27530 | 0.810008 | 1.43E-50 | -1.69E-46 |
| MS.gene060297 | MS.gene27530 | 0.804629 | 1.97E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27530.t1 | MTR_7g074250 | 92.891 | 211 | 15 | 0 | 1 | 211 | 1 | 211 | 2.01e-128 | 360 |
| MS.gene27530.t1 | MTR_8g005060 | 36.126 | 191 | 117 | 2 | 21 | 211 | 7 | 192 | 4.29e-33 | 118 |
| MS.gene27530.t1 | MTR_8g028475 | 36.765 | 204 | 120 | 5 | 11 | 211 | 22 | 219 | 1.18e-25 | 99.4 |
| MS.gene27530.t1 | MTR_2g088610 | 27.027 | 185 | 124 | 4 | 29 | 211 | 9 | 184 | 3.49e-15 | 71.2 |
| MS.gene27530.t1 | MTR_5g005950 | 28.395 | 162 | 110 | 4 | 50 | 211 | 35 | 190 | 5.67e-12 | 62.4 |
| MS.gene27530.t1 | MTR_5g009130 | 25.698 | 179 | 125 | 3 | 19 | 197 | 9 | 179 | 2.00e-11 | 60.8 |
| MS.gene27530.t1 | MTR_2g088680 | 23.560 | 191 | 138 | 2 | 22 | 211 | 2 | 185 | 3.30e-11 | 60.5 |
| MS.gene27530.t1 | MTR_8g099035 | 29.293 | 198 | 129 | 5 | 3 | 200 | 29 | 215 | 6.53e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27530.t1 | AT3G05975 | 33.690 | 187 | 123 | 1 | 25 | 211 | 4 | 189 | 6.99e-36 | 125 |
| MS.gene27530.t1 | AT3G54200 | 31.579 | 228 | 136 | 2 | 3 | 211 | 9 | 235 | 1.93e-19 | 83.6 |
| MS.gene27530.t1 | AT2G46150 | 24.510 | 204 | 150 | 4 | 10 | 211 | 20 | 221 | 2.02e-15 | 72.4 |
| MS.gene27530.t1 | AT4G23930 | 24.309 | 181 | 124 | 2 | 32 | 205 | 13 | 187 | 3.58e-11 | 60.1 |
Find 22 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCCTTATTCTTGCAGATT+TGG | 0.252406 | 7.3:-30923587 | None:intergenic |
| TCATCAAGGCTTGTTCTTCT+TGG | 0.289565 | 7.3:-30923008 | None:intergenic |
| AATGGGTGGTCAACTCTCTT+TGG | 0.325009 | 7.3:+30923449 | MS.gene27530:CDS |
| AAGCCAAATCTGCAAGAATA+AGG | 0.332506 | 7.3:+30923584 | MS.gene27530:CDS |
| CCAAGAAAAGTAACTCTTCC+AGG | 0.349074 | 7.3:-30923489 | None:intergenic |
| GAAGCAGTTACTAGGATTCC+TGG | 0.355010 | 7.3:+30923471 | MS.gene27530:CDS |
| GCTGAAACAACCGTTGCTTT+TGG | 0.387060 | 7.3:-30923150 | None:intergenic |
| TCTCTTTGGAAGCAGTTACT+AGG | 0.401657 | 7.3:+30923463 | MS.gene27530:CDS |
| GTTGTTTCAGCTACTTTAGA+AGG | 0.419593 | 7.3:+30923162 | MS.gene27530:CDS |
| ATGCTAATTTATCAGCTTGA+AGG | 0.435205 | 7.3:-30923398 | None:intergenic |
| ATGTATAAAGGTGTTGAAGT+TGG | 0.503450 | 7.3:+30923309 | MS.gene27530:CDS |
| AAATTAGCATCCAATGTGAC+AGG | 0.508296 | 7.3:+30923411 | MS.gene27530:CDS |
| CTGCATGTTTGAAGCTTACA+CGG | 0.519734 | 7.3:-30923269 | None:intergenic |
| TGCTAATTTATCAGCTTGAA+GGG | 0.525961 | 7.3:-30923397 | None:intergenic |
| TTGAGTTGTATGTCAATAGC+AGG | 0.526566 | 7.3:-30923207 | None:intergenic |
| GGAGACACAGATATATATCC+TGG | 0.534807 | 7.3:+30923330 | MS.gene27530:CDS |
| GCTTGAAGGGTAAGTCTACA+AGG | 0.570776 | 7.3:-30923384 | None:intergenic |
| CCTGGTTTGATTGCTGCAAG+AGG | 0.591703 | 7.3:+30923348 | MS.gene27530:CDS |
| GTAAGCTTCAAACATGCAGA+TGG | 0.601840 | 7.3:+30923273 | MS.gene27530:CDS |
| CCTCTTGCAGCAATCAAACC+AGG | 0.602161 | 7.3:-30923348 | None:intergenic |
| GCCAAAACAACCAAAAGCAA+CGG | 0.624220 | 7.3:+30923140 | MS.gene27530:CDS |
| ATAGCAGGAAATGTTAGACG+AGG | 0.747015 | 7.3:-30923192 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTAATTGTCAATTTGTTTT+TGG | + | chr7.3:30923540-30923559 | MS.gene27530:CDS | 20.0% |
| !!! | ACTAGTCTTTTGATGTATAA+AGG | + | chr7.3:30923297-30923316 | MS.gene27530:CDS | 25.0% |
| !!! | AGGTTTTTTAGGTGATTTAA+TGG | + | chr7.3:30923431-30923450 | MS.gene27530:CDS | 25.0% |
| !!! | GGTTTTTTAGGTGATTTAAT+GGG | + | chr7.3:30923432-30923451 | MS.gene27530:CDS | 25.0% |
| ATGCTAATTTATCAGCTTGA+AGG | - | chr7.3:30923401-30923420 | None:intergenic | 30.0% | |
| TGCTAATTTATCAGCTTGAA+GGG | - | chr7.3:30923400-30923419 | None:intergenic | 30.0% | |
| TTGATGAATCCACCAAAAAA+GGG | + | chr7.3:30923024-30923043 | MS.gene27530:CDS | 30.0% | |
| !! | ATGTATAAAGGTGTTGAAGT+TGG | + | chr7.3:30923309-30923328 | MS.gene27530:CDS | 30.0% |
| !!! | TGTTTTTGGTTTTCCTGATA+TGG | + | chr7.3:30923554-30923573 | MS.gene27530:CDS | 30.0% |
| !!! | TTTTTAGGTGATTTAATGGG+TGG | + | chr7.3:30923435-30923454 | MS.gene27530:CDS | 30.0% |
| AAATTAGCATCCAATGTGAC+AGG | + | chr7.3:30923411-30923430 | MS.gene27530:CDS | 35.0% | |
| AAGCCAAATCTGCAAGAATA+AGG | + | chr7.3:30923584-30923603 | MS.gene27530:CDS | 35.0% | |
| ACCTAAAAAACCTGTCACAT+TGG | - | chr7.3:30923424-30923443 | None:intergenic | 35.0% | |
| CTTGATGAATCCACCAAAAA+AGG | + | chr7.3:30923023-30923042 | MS.gene27530:CDS | 35.0% | |
| ! | GTTGTTTCAGCTACTTTAGA+AGG | + | chr7.3:30923162-30923181 | MS.gene27530:CDS | 35.0% |
| ! | TTGAGTTGTATGTCAATAGC+AGG | - | chr7.3:30923210-30923229 | None:intergenic | 35.0% |
| !! | TAGCCTTATTCTTGCAGATT+TGG | - | chr7.3:30923590-30923609 | None:intergenic | 35.0% |
| !!! | CATCTGTTTCTTCCCTTTTT+TGG | - | chr7.3:30923039-30923058 | None:intergenic | 35.0% |
| !!! | CTTTTTTGGTGGATTCATCA+AGG | - | chr7.3:30923025-30923044 | None:intergenic | 35.0% |
| !!! | TCCAATGTGACAGGTTTTTT+AGG | + | chr7.3:30923420-30923439 | MS.gene27530:CDS | 35.0% |
| !!! | TGGCTTTTGATTTCCATATC+AGG | - | chr7.3:30923570-30923589 | None:intergenic | 35.0% |
| !!! | TGGTTGTTTTGGCTTGAAAA+CGG | - | chr7.3:30923133-30923152 | None:intergenic | 35.0% |
| !!! | TTTTGGCTTGAAAACGGTTA+AGG | - | chr7.3:30923127-30923146 | None:intergenic | 35.0% |
| ATAGCAGGAAATGTTAGACG+AGG | - | chr7.3:30923195-30923214 | None:intergenic | 40.0% | |
| CCAAGAAAAGTAACTCTTCC+AGG | - | chr7.3:30923492-30923511 | None:intergenic | 40.0% | |
| GCCAAAACAACCAAAAGCAA+CGG | + | chr7.3:30923140-30923159 | MS.gene27530:CDS | 40.0% | |
| GGAGACACAGATATATATCC+TGG | + | chr7.3:30923330-30923349 | MS.gene27530:CDS | 40.0% | |
| GTAAGCTTCAAACATGCAGA+TGG | + | chr7.3:30923273-30923292 | MS.gene27530:CDS | 40.0% | |
| ! | CTGCATGTTTGAAGCTTACA+CGG | - | chr7.3:30923272-30923291 | None:intergenic | 40.0% |
| ! | TCATCAAGGCTTGTTCTTCT+TGG | - | chr7.3:30923011-30923030 | None:intergenic | 40.0% |
| ! | TCTCTTTGGAAGCAGTTACT+AGG | + | chr7.3:30923463-30923482 | MS.gene27530:CDS | 40.0% |
| !!! | ACCGTTGCTTTTGGTTGTTT+TGG | - | chr7.3:30923144-30923163 | None:intergenic | 40.0% |
| !!! | CCTGGAAGAGTTACTTTTCT+TGG | + | chr7.3:30923489-30923508 | MS.gene27530:CDS | 40.0% |
| !!! | CTGTTTCTTCCCTTTTTTGG+TGG | - | chr7.3:30923036-30923055 | None:intergenic | 40.0% |
| AATGGGTGGTCAACTCTCTT+TGG | + | chr7.3:30923449-30923468 | MS.gene27530:CDS | 45.0% | |
| GAAGCAGTTACTAGGATTCC+TGG | + | chr7.3:30923471-30923490 | MS.gene27530:CDS | 45.0% | |
| GCTTGAAGGGTAAGTCTACA+AGG | - | chr7.3:30923387-30923406 | None:intergenic | 45.0% | |
| !! | GCTGAAACAACCGTTGCTTT+TGG | - | chr7.3:30923153-30923172 | None:intergenic | 45.0% |
| CCTCTTGCAGCAATCAAACC+AGG | - | chr7.3:30923351-30923370 | None:intergenic | 50.0% | |
| ! | CCTGGTTTGATTGCTGCAAG+AGG | + | chr7.3:30923348-30923367 | MS.gene27530:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 30922982 | 30923617 | 30922982 | ID=MS.gene27530 |
| chr7.3 | mRNA | 30922982 | 30923617 | 30922982 | ID=MS.gene27530.t1;Parent=MS.gene27530 |
| chr7.3 | exon | 30922982 | 30923617 | 30922982 | ID=MS.gene27530.t1.exon1;Parent=MS.gene27530.t1 |
| chr7.3 | CDS | 30922982 | 30923617 | 30922982 | ID=cds.MS.gene27530.t1;Parent=MS.gene27530.t1 |
| Gene Sequence |
| Protein sequence |
