AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28832


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28832.t1 MTR_7g109850 95.872 218 7 1 1 218 1 216 8.48e-152 420
MS.gene28832.t1 MTR_8g038785 87.313 134 15 1 1 134 1 132 1.12e-74 236
MS.gene28832.t1 MTR_2g005900 42.308 156 84 3 66 216 68 222 1.06e-29 110
MS.gene28832.t1 MTR_2g036690 37.267 161 99 2 51 210 71 230 7.38e-24 95.5
MS.gene28832.t1 MTR_2g036690 44.248 113 59 2 51 160 71 182 7.57e-20 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28832.t1 AT4G01940 67.822 202 55 3 25 218 32 231 4.89e-89 262
MS.gene28832.t1 AT5G49940 35.897 195 109 5 27 216 51 234 3.88e-26 101
MS.gene28832.t1 AT4G25910 33.180 217 128 6 12 212 11 226 9.38e-24 95.1
MS.gene28832.t1 AT5G49940 53.165 79 35 1 71 149 85 161 1.71e-21 87.8

Find 44 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAACCACAACTATGAAAA+TGG 0.150095 7.4:-5585265 MS.gene28832:CDS
CACTTGCACGATATCTTTAA+TGG 0.162531 7.4:+5585206 None:intergenic
CGTGTTCTTAAGGAGAAATT+TGG 0.227866 7.4:-5585234 MS.gene28832:CDS
TTCGTTGGTATTGAACAAAT+TGG 0.356289 7.4:+5585600 None:intergenic
ATCCCGGAGAAGACTGGTTC+TGG 0.389633 7.4:+5585451 None:intergenic
AAGGTAACATTGAGAATATC+TGG 0.389748 7.4:+5584156 None:intergenic
CTGGTTCTGGTTAGCGGTAG+AGG 0.412467 7.4:+5585464 None:intergenic
GGGAATCGAACGTGTTCTTA+AGG 0.422078 7.4:-5585244 MS.gene28832:CDS
TAACCAGAACCAGTCTTCTC+CGG 0.444988 7.4:-5585454 MS.gene28832:CDS
TCAACCACAACTATGAAAAT+GGG 0.480712 7.4:-5585264 MS.gene28832:CDS
AGTATAATCCCGGAGAAGAC+TGG 0.483617 7.4:+5585445 None:intergenic
GGACCTGAGTCCATTGGATC+AGG 0.493608 7.4:-5584207 MS.gene28832:CDS
AAATTCAAATTCAGTTTCGT+TGG 0.499228 7.4:+5585585 None:intergenic
AGGTGTTAAAGCTGCGATCA+AGG 0.501612 7.4:-5584187 MS.gene28832:CDS
GATGTTGTATCCGTTGAAAA+CGG 0.506210 7.4:-5585333 MS.gene28832:CDS
TTCATAGTTGTGGTTGAGCT+AGG 0.507201 7.4:+5585270 None:intergenic
AACGGTGAGGTCGAATTTCG+TGG 0.510902 7.4:+5585419 None:intergenic
CCTCCGTCAGAGATGAGGTA+AGG 0.518971 7.4:+5585363 None:intergenic
TATGTTGGACCTGAGTCCAT+TGG 0.522225 7.4:-5584213 MS.gene28832:CDS
CGCCCTTACCTCATCTCTGA+CGG 0.525777 7.4:-5585366 MS.gene28832:CDS
AGAAGACTGGTTCTGGTTAG+CGG 0.534911 7.4:+5585458 None:intergenic
AACCAGAACCAGTCTTCTCC+GGG 0.537520 7.4:-5585453 MS.gene28832:CDS
TTCGTGGCGGAGTATAATCC+CGG 0.541925 7.4:+5585435 None:intergenic
GTCGTTTCTCTTAAGCTACA+AGG 0.546334 7.4:-5585309 MS.gene28832:CDS
AACGAGATCTACGTTTGAAA+CGG 0.549378 7.4:+5585401 None:intergenic
AATTGCTACCTAGGTGGAGA+AGG 0.550345 7.4:+5584137 None:intergenic
GACTGTGTTGTGGAATATGT+TGG 0.562518 7.4:-5584228 MS.gene28832:CDS
AGCAAGGGAAACAACGGTTG+AGG 0.563035 7.4:-5585169 MS.gene28832:intron
TGAAGGTGGTGACTGTGTTG+TGG 0.573198 7.4:-5584238 MS.gene28832:CDS
AGCTTTAACACCTGATCCAA+TGG 0.583998 7.4:+5584197 None:intergenic
CTCCGTCAGAGATGAGGTAA+GGG 0.586257 7.4:+5585364 None:intergenic
CGTTTCCTCCGTCAGAGATG+AGG 0.588496 7.4:+5585358 None:intergenic
CAATGTTACCTTCTCCACCT+AGG 0.589162 7.4:-5584145 None:intergenic
GGTTAGCGGTAGAGGATGAA+CGG 0.599940 7.4:+5585472 None:intergenic
TGTTTGATGATGATCAAGCA+AGG 0.620834 7.4:-5585185 MS.gene28832:CDS
GTTTGATGATGATCAAGCAA+GGG 0.625912 7.4:-5585184 MS.gene28832:CDS
CCTTACCTCATCTCTGACGG+AGG 0.627247 7.4:-5585363 MS.gene28832:CDS
TCATCTAGAGATACTGAGAC+CGG 0.630070 7.4:-5584301 MS.gene28832:CDS
ACACCTGATCCAATGGACTC+AGG 0.634250 7.4:+5584204 None:intergenic
GAATTAAATTGCTACCTAGG+TGG 0.648629 7.4:+5584131 None:intergenic
TGATCAAGCAAGGGAAACAA+CGG 0.689446 7.4:-5585175 MS.gene28832:CDS
GATCTACGTTTGAAACGGTG+AGG 0.706207 7.4:+5585406 None:intergenic
AGAGGATGAACGGATTACTG+TGG 0.730832 7.4:+5585482 None:intergenic
GGTGAGGTCGAATTTCGTGG+CGG 0.777998 7.4:+5585422 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTACTATTATTTATCTTTTA+TGG - chr7.4:5584643-5584662 MS.gene28832:intron 10.0%
!!! AATGTTTTTATAACCTATAT+TGG - chr7.4:5584924-5584943 MS.gene28832:intron 15.0%
!! AATAGTAAATGCAAAAAGTA+AGG + chr7.4:5584631-5584650 None:intergenic 20.0%
!! ATAGTAAATGCAAAAAGTAA+GGG + chr7.4:5584630-5584649 None:intergenic 20.0%
!! ATATTAGTTAACATCATGAA+AGG + chr7.4:5584789-5584808 None:intergenic 20.0%
!! TTGGATAAATAACTTATTTG+CGG - chr7.4:5584824-5584843 MS.gene28832:intron 20.0%
!!! TGATATTGTGAAATTGATTT+TGG + chr7.4:5585291-5585310 None:intergenic 20.0%
! AAATTCAAATTCAGTTTCGT+TGG + chr7.4:5584179-5584198 None:intergenic 25.0%
! TAGTAAATGCAAAAAGTAAG+GGG + chr7.4:5584629-5584648 None:intergenic 25.0%
! TGAAACCAAACATAAACTTA+GGG + chr7.4:5585046-5585065 None:intergenic 25.0%
! TGTTAACTAATATAGCAACT+AGG - chr7.4:5584796-5584815 MS.gene28832:intron 25.0%
! TTAGAATCAGTGTATGTTAT+TGG + chr7.4:5585347-5585366 None:intergenic 25.0%
!! ATGAATGTTAGTTGATGTTT+GGG - chr7.4:5585412-5585431 MS.gene28832:CDS 25.0%
!! CTTTTATGGTTGTTAATGAT+TGG - chr7.4:5584657-5584676 MS.gene28832:intron 25.0%
!!! TTCCTTTTTAAGCTAGAAAT+TGG - chr7.4:5585161-5585180 MS.gene28832:intron 25.0%
AAGGTAACATTGAGAATATC+TGG + chr7.4:5585608-5585627 None:intergenic 30.0%
AGACCGGCAATTAAAAATTA+CGG - chr7.4:5585476-5585495 MS.gene28832:CDS 30.0%
CCTATATTGGAGAACTTATA+AGG - chr7.4:5584937-5584956 MS.gene28832:intron 30.0%
CCTTATAAGTTCTCCAATAT+AGG + chr7.4:5584940-5584959 None:intergenic 30.0%
GCTTAAAAAGGAATCAATTC+TGG + chr7.4:5585154-5585173 None:intergenic 30.0%
GTGAAACCAAACATAAACTT+AGG + chr7.4:5585047-5585066 None:intergenic 30.0%
TAAAAAGGAATCAATTCTGG+AGG + chr7.4:5585151-5585170 None:intergenic 30.0%
TCAACCACAACTATGAAAAT+GGG - chr7.4:5584497-5584516 MS.gene28832:intron 30.0%
! GAAGTTCTTGAATTTTGCTT+TGG + chr7.4:5584257-5584276 None:intergenic 30.0%
! GATGAATGTTAGTTGATGTT+TGG - chr7.4:5585411-5585430 MS.gene28832:CDS 30.0%
! TTCGTTGGTATTGAACAAAT+TGG + chr7.4:5584164-5584183 None:intergenic 30.0%
! TTTGGTTAAAAGTGAGTTGA+AGG + chr7.4:5585273-5585292 None:intergenic 30.0%
!! CAAAAGTGATTTTAAAGGTG+TGG - chr7.4:5585079-5585098 MS.gene28832:intron 30.0%
!! CTCCAATTTCTAGCTTAAAA+AGG + chr7.4:5585166-5585185 None:intergenic 30.0%
!!! CACACCTTTAAAATCACTTT+TGG + chr7.4:5585081-5585100 None:intergenic 30.0%
!!! TGAGATTGTGTTGTTTTTGT+TGG + chr7.4:5584208-5584227 None:intergenic 30.0%
AACGAGATCTACGTTTGAAA+CGG + chr7.4:5584363-5584382 None:intergenic 35.0%
ATATAGCAACTAGGTCTGTT+TGG - chr7.4:5584805-5584824 MS.gene28832:intron 35.0%
CACTTGCACGATATCTTTAA+TGG + chr7.4:5584558-5584577 None:intergenic 35.0%
CGTGTTCTTAAGGAGAAATT+TGG - chr7.4:5584527-5584546 MS.gene28832:intron 35.0%
CTCAACCACAACTATGAAAA+TGG - chr7.4:5584496-5584515 MS.gene28832:intron 35.0%
GATGTTGTATCCGTTGAAAA+CGG - chr7.4:5584428-5584447 MS.gene28832:intron 35.0%
GTTGAAGGTAAACTGAATGA+TGG + chr7.4:5585258-5585277 None:intergenic 35.0%
! ATGTTTGGTTTCACTTCTAG+AGG - chr7.4:5585053-5585072 MS.gene28832:intron 35.0%
! GATTCCCATTTTCATAGTTG+TGG + chr7.4:5584504-5584523 None:intergenic 35.0%
! TGTTTGGTTTCACTTCTAGA+GGG - chr7.4:5585054-5585073 MS.gene28832:intron 35.0%
!! CAAGTTTTATCTGTTGAAGG+TGG - chr7.4:5585509-5585528 MS.gene28832:CDS 35.0%
!! CCTCCGTAATTTTTAATTGC+CGG + chr7.4:5585482-5585501 None:intergenic 35.0%
!! GTGCAAGTTTTATCTGTTGA+AGG - chr7.4:5585506-5585525 MS.gene28832:CDS 35.0%
!! GTTTGATGATGATCAAGCAA+GGG - chr7.4:5584577-5584596 MS.gene28832:intron 35.0%
!! TGTTTGATGATGATCAAGCA+AGG - chr7.4:5584576-5584595 MS.gene28832:intron 35.0%
AACTTAGGGCCAGTTTGAAT+TGG + chr7.4:5585032-5585051 None:intergenic 40.0%
ACTGGCCCTAAGTTTATGTT+TGG - chr7.4:5585038-5585057 MS.gene28832:intron 40.0%
CCGGCAATTAAAAATTACGG+AGG - chr7.4:5585479-5585498 MS.gene28832:CDS 40.0%
GACTGTGTTGTGGAATATGT+TGG - chr7.4:5585533-5585552 MS.gene28832:CDS 40.0%
GTCGTTTCTCTTAAGCTACA+AGG - chr7.4:5584452-5584471 MS.gene28832:intron 40.0%
TCAGATACGCCAATTCAAAC+TGG - chr7.4:5585020-5585039 MS.gene28832:intron 40.0%
TCATCTAGAGATACTGAGAC+CGG - chr7.4:5585460-5585479 MS.gene28832:CDS 40.0%
TGATCAAGCAAGGGAAACAA+CGG - chr7.4:5584586-5584605 MS.gene28832:intron 40.0%
TTCATAGTTGTGGTTGAGCT+AGG + chr7.4:5584494-5584513 None:intergenic 40.0%
! AGCTTTAACACCTGATCCAA+TGG + chr7.4:5585567-5585586 None:intergenic 40.0%
! GGGGCCAAAAGTGATTTTAA+AGG - chr7.4:5585074-5585093 MS.gene28832:intron 40.0%
! GTTAGTTGATGTTTGGGTGT+GGG - chr7.4:5585418-5585437 MS.gene28832:CDS 40.0%
! GTTTGGTTTCACTTCTAGAG+GGG - chr7.4:5585055-5585074 MS.gene28832:intron 40.0%
! TGTTAGTTGATGTTTGGGTG+TGG - chr7.4:5585417-5585436 MS.gene28832:CDS 40.0%
!!! TGTTTGGGTGTGGGTTTTTT+AGG - chr7.4:5585427-5585446 MS.gene28832:CDS 40.0%
AGGTGTTAAAGCTGCGATCA+AGG - chr7.4:5585574-5585593 MS.gene28832:CDS 45.0%
AGTATAATCCCGGAGAAGAC+TGG + chr7.4:5584319-5584338 None:intergenic 45.0%
GAGCTCATCAATTGGCGTTT+GGG + chr7.4:5585002-5585021 None:intergenic 45.0%
GCGTATCTGAGCTCATCAAT+TGG + chr7.4:5585010-5585029 None:intergenic 45.0%
GGGAATCGAACGTGTTCTTA+AGG - chr7.4:5584517-5584536 MS.gene28832:intron 45.0%
TAACCAGAACCAGTCTTCTC+CGG - chr7.4:5584307-5584326 MS.gene28832:CDS 45.0%
TATGTTGGACCTGAGTCCAT+TGG - chr7.4:5585548-5585567 MS.gene28832:CDS 45.0%
TGAGCTCATCAATTGGCGTT+TGG + chr7.4:5585003-5585022 None:intergenic 45.0%
! GATCTACGTTTGAAACGGTG+AGG + chr7.4:5584358-5584377 None:intergenic 45.0%
!! AGAAACGACGCCGTTTTCAA+CGG + chr7.4:5584441-5584460 None:intergenic 45.0%
!! AGAAGACTGGTTCTGGTTAG+CGG + chr7.4:5584306-5584325 None:intergenic 45.0%
!! AGAGGATGAACGGATTACTG+TGG + chr7.4:5584282-5584301 None:intergenic 45.0%
!!! TTGGGTGTGGGTTTTTTAGG+CGG - chr7.4:5585430-5585449 MS.gene28832:CDS 45.0%
AACCAGAACCAGTCTTCTCC+GGG - chr7.4:5584308-5584327 MS.gene28832:CDS 50.0%
AACGGTGAGGTCGAATTTCG+TGG + chr7.4:5584345-5584364 None:intergenic 50.0%
AGCAAGGGAAACAACGGTTG+AGG - chr7.4:5584592-5584611 MS.gene28832:intron 50.0%
CTCCGTCAGAGATGAGGTAA+GGG + chr7.4:5584400-5584419 None:intergenic 50.0%
GGTTAGCGGTAGAGGATGAA+CGG + chr7.4:5584292-5584311 None:intergenic 50.0%
TGAAGGTGGTGACTGTGTTG+TGG - chr7.4:5585523-5585542 MS.gene28832:CDS 50.0%
! ACACCTGATCCAATGGACTC+AGG + chr7.4:5585560-5585579 None:intergenic 50.0%
! TTCGTGGCGGAGTATAATCC+CGG + chr7.4:5584329-5584348 None:intergenic 50.0%
CCTCCGTCAGAGATGAGGTA+AGG + chr7.4:5584401-5584420 None:intergenic 55.0%
CCTTACCTCATCTCTGACGG+AGG - chr7.4:5584398-5584417 MS.gene28832:intron 55.0%
CGCCCTTACCTCATCTCTGA+CGG - chr7.4:5584395-5584414 MS.gene28832:intron 55.0%
CGTTTCCTCCGTCAGAGATG+AGG + chr7.4:5584406-5584425 None:intergenic 55.0%
GGACCTGAGTCCATTGGATC+AGG - chr7.4:5585554-5585573 MS.gene28832:CDS 55.0%
GGTGAGGTCGAATTTCGTGG+CGG + chr7.4:5584342-5584361 None:intergenic 55.0%
! ATCCCGGAGAAGACTGGTTC+TGG + chr7.4:5584313-5584332 None:intergenic 55.0%
!! CTGGTTCTGGTTAGCGGTAG+AGG + chr7.4:5584300-5584319 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 5584146 5585637 5584146 ID=MS.gene28832
chr7.4 mRNA 5584146 5585637 5584146 ID=MS.gene28832.t1;Parent=MS.gene28832
chr7.4 exon 5585170 5585637 5585170 ID=MS.gene28832.t1.exon1;Parent=MS.gene28832.t1
chr7.4 CDS 5585170 5585637 5585170 ID=cds.MS.gene28832.t1;Parent=MS.gene28832.t1
chr7.4 exon 5584146 5584334 5584146 ID=MS.gene28832.t1.exon2;Parent=MS.gene28832.t1
chr7.4 CDS 5584146 5584334 5584146 ID=cds.MS.gene28832.t1;Parent=MS.gene28832.t1
Gene Sequence

>MS.gene28832

ATGGCGACTCTAACTCCAATTTGTTCAATACCAACGAAACTGAATTTGAATTTGAGTATCCAACAAAAACAACACAATCTCAACTTCGCACTTTTCAATTTCAAAAATCCAAAGCAAAATTCAAGAACTTCAACCACAGTAATCCGTTCATCCTCTACCGCTAACCAGAACCAGTCTTCTCCGGGATTATACTCCGCCACGAAATTCGACCTCACCGTTTCAAACGTAGATCTCGTTCTCGAAGATGTTCGCCCTTACCTCATCTCTGACGGAGGAAACGTCGATGTTGTATCCGTTGAAAACGGCGTCGTTTCTCTTAAGCTACAAGGTGCATGCGAAAGTTGTCCTAGCTCAACCACAACTATGAAAATGGGAATCGAACGTGTTCTTAAGGAGAAATTTGGAGATTCCATTAAAGATATCGTGCAAGTGTTTGATGATGATCAAGCAAGGGAAACAACGGTTGAGGTCAGTGATTTTCCCCTTACTTTTTGCATTTACTATTATTTATCTTTTATGGTTGTTAATGATTGGATATTAATTTTAGTTAGTCTTATAGAAGTAGTTAGAAGTTAGATGTGATTAGTTGTTGTTAGTTAATTAGTTACATCACATATGAAGTGATCAATTTCAAAATCAACCTTTCATGATGTTAACTAATATAGCAACTAGGTCTGTTTGGATAAATAACTTATTTGCGGTTTATAGCACAATCACTTATAAGCTATTTTTGTAGCAAAAGATAAAATGAAGTTAAATTGTATTTGTATATGCTATAAATGTTTTTATAACCTATATTGGAGAACTTATAAGGTTAAGTTGTAAAAAGCTTATGAAAATTGTCGTAAGTTTTCCCAAACGCCAATTGATGAGCTCAGATACGCCAATTCAAACTGGCCCTAAGTTTATGTTTGGTTTCACTTCTAGAGGGGCCAAAAGTGATTTTAAAGGTGTGGAATTGATTTTGACATGTTTGATTACTCTCTACTATAATTGATTTTGCCTCCAGAATTGATTCCTTTTTAAGCTAGAAATTGGAGTTATAATTATAGAATTGATTTTAGCACTCAAATCTACTGTTCAACTCACTTTTACTTAAATGTAACAAACCATCATTCAGTTTACCTTCAACTCACTTTTAACCAAAATCAATTTCACAATATCAATTCACGCACAATCAATTTATGTCAATGCAAAACCAATAACATACACTGATTCTAATTAATATTCAATTTATCTAATGTGCATAGAAATGAAATGAAAATGATGAATGTTAGTTGATGTTTGGGTGTGGGTTTTTTAGGCGGTGAACAATCATCTAGAGATACTGAGACCGGCAATTAAAAATTACGGAGGTAGTGTGCAAGTTTTATCTGTTGAAGGTGGTGACTGTGTTGTGGAATATGTTGGACCTGAGTCCATTGGATCAGGTGTTAAAGCTGCGATCAAGGAGAAGTTTCCAGATATTCTCAATGTTACCTTCTCCACCTAG

Protein sequence

>MS.gene28832.t1

MATLTPICSIPTKLNLNLSIQQKQHNLNFALFNFKNPKQNSRTSTTVIRSSSTANQNQSSPGLYSATKFDLTVSNVDLVLEDVRPYLISDGGNVDVVSVENGVVSLKLQGACESCPSSTTTMKMGIERVLKEKFGDSIKDIVQVFDDDQARETTVEAVNNHLEILRPAIKNYGGSVQVLSVEGGDCVVEYVGPESIGSGVKAAIKEKFPDILNVTFST