AlfalfaGEDB | MS.gene28842

M. sativa cultivar XinJiangDaYe / MS.gene28842

KEGG: -
GO: -
PFAM: PF06108.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28842.t1	XP_024625665.1	98.5	67	1	0	1	67	1	67	2.50E-28	134

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28842.t1	G7KTP9	98.5	67	1	0	1	67	1	67	1.8e-28	134.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene28842	0.849215	3.73E-60	-1.69E-46
MS.gene049501	MS.gene28842	0.814528	1.47E-51	-1.69E-46
MS.gene050020	MS.gene28842	0.814068	1.86E-51	-1.69E-46
MS.gene050689	MS.gene28842	0.810767	9.79E-51	-1.69E-46
MS.gene050807	MS.gene28842	0.817766	2.78E-52	-1.69E-46
MS.gene050815	MS.gene28842	0.803793	2.94E-49	-1.69E-46
MS.gene051243	MS.gene28842	0.812862	3.42E-51	-1.69E-46
MS.gene051328	MS.gene28842	0.848825	4.79E-60	-1.69E-46
MS.gene052020	MS.gene28842	0.81475	1.31E-51	-1.69E-46
MS.gene052516	MS.gene28842	0.804702	1.90E-49	-1.69E-46
MS.gene052611	MS.gene28842	0.815193	1.05E-51	-1.69E-46
MS.gene052665	MS.gene28842	0.826081	3.30E-54	-1.69E-46
MS.gene052863	MS.gene28842	0.820217	7.71E-53	-1.69E-46
MS.gene05288	MS.gene28842	0.80687	6.67E-50	-1.69E-46
MS.gene053008	MS.gene28842	0.84379	1.13E-58	-1.69E-46
MS.gene05510	MS.gene28842	0.809411	1.92E-50	-1.69E-46
MS.gene05517	MS.gene28842	0.806273	8.91E-50	-1.69E-46
MS.gene055512	MS.gene28842	0.807786	4.26E-50	-1.69E-46
MS.gene055623	MS.gene28842	0.809975	1.45E-50	-1.69E-46
MS.gene055926	MS.gene28842	0.800369	1.49E-48	-1.69E-46
MS.gene056553	MS.gene28842	0.805731	1.16E-49	-1.69E-46
MS.gene056760	MS.gene28842	0.822971	1.78E-53	-1.69E-46
MS.gene057140	MS.gene28842	0.808105	3.65E-50	-1.69E-46
MS.gene057444	MS.gene28842	0.802709	4.93E-49	-1.69E-46
MS.gene057693	MS.gene28842	0.813186	2.91E-51	-1.69E-46
MS.gene057869	MS.gene28842	0.813598	2.36E-51	-1.69E-46
MS.gene058010	MS.gene28842	0.802877	4.55E-49	-1.69E-46
MS.gene059842	MS.gene28842	0.832025	1.20E-55	-1.69E-46
MS.gene059937	MS.gene28842	0.800084	1.70E-48	-1.69E-46
MS.gene060310	MS.gene28842	0.831707	1.43E-55	-1.69E-46
MS.gene060322	MS.gene28842	0.812028	5.21E-51	-1.69E-46
MS.gene060802	MS.gene28842	0.831012	2.12E-55	-1.69E-46
MS.gene060803	MS.gene28842	0.842591	2.35E-58	-1.69E-46
MS.gene060998	MS.gene28842	0.803696	3.08E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28842.t1	MTR_7g109980	98.507	67	1	0	1	67	1	67	5.05e-43	135

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28842.t1	AT4G01897	61.538	65	25	0	3	67	5	69	1.36e-26	94.0

Find 13 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTGATCTAGTTTGCTAAGA+TGG	0.325847	7.4:+5500987	None:intergenic
CAAAGAAATGGGTCCACTTT+CGG	0.360708	7.4:-5501040	MS.gene28842:CDS
TGGGAAGAATTGCAAAGAAA+TGG	0.392074	7.4:-5501052	MS.gene28842:CDS
TCTTCAGATTGATGCTAAGA+AGG	0.429001	7.4:-5500780	MS.gene28842:intron
CCATCTTAGCAAACTAGATC+AGG	0.463319	7.4:-5500987	MS.gene28842:intron
GTCAAGTTCACCACCGAAAG+TGG	0.465358	7.4:+5501027	None:intergenic
AATCAGCACGGCGAAGGAAT+GGG	0.486850	7.4:-5501071	MS.gene28842:CDS
GGGAAGAATTGCAAAGAAAT+GGG	0.488668	7.4:-5501051	MS.gene28842:CDS
GAATCAGCACGGCGAAGGAA+TGG	0.494049	7.4:-5501072	MS.gene28842:CDS
TCTTAGCATCAATCTGAAGA+AGG	0.553114	7.4:+5500783	None:intergenic
GTACAGAATCAGCACGGCGA+AGG	0.581959	7.4:-5501077	MS.gene28842:CDS
ATTGCAGGTTCGATCAACGT+TGG	0.585116	7.4:-5500840	MS.gene28842:intron
AGAAATGGGTCCACTTTCGG+TGG	0.639639	7.4:-5501037	MS.gene28842:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATAATTATTTTATTATTGC+AGG	-	chr7.4:5500919-5500938	MS.gene28842:intron	10.0%
!!	TAATTAAAAAACATGCTTAT+TGG	-	chr7.4:5501082-5501101	MS.gene28842:CDS	15.0%
!!!	TAAAAAAAAGCATAGTGAAA+GGG	+	chr7.4:5501033-5501052	None:intergenic	20.0%
!!!	TTAAAAAAAAGCATAGTGAA+AGG	+	chr7.4:5501034-5501053	None:intergenic	20.0%
	TATAAGTCGCTTCATTCATT+CGG	-	chr7.4:5500877-5500896	MS.gene28842:intron	30.0%
	GGGAAGAATTGCAAAGAAAT+GGG	-	chr7.4:5500723-5500742	MS.gene28842:intron	35.0%
	TCTTAGCATCAATCTGAAGA+AGG	+	chr7.4:5500994-5501013	None:intergenic	35.0%
	TCTTCAGATTGATGCTAAGA+AGG	-	chr7.4:5500994-5501013	MS.gene28842:CDS	35.0%
	TGGGAAGAATTGCAAAGAAA+TGG	-	chr7.4:5500722-5500741	MS.gene28842:intron	35.0%
!	TTTTGTGTACAGAATCAGCA+CGG	-	chr7.4:5500691-5500710	MS.gene28842:intron	35.0%
	CAAAGAAATGGGTCCACTTT+CGG	-	chr7.4:5500734-5500753	MS.gene28842:intron	40.0%
	CCATCTTAGCAAACTAGATC+AGG	-	chr7.4:5500787-5500806	MS.gene28842:CDS	40.0%
!!	CCTGATCTAGTTTGCTAAGA+TGG	+	chr7.4:5500790-5500809	None:intergenic	40.0%
	ATTGCAGGTTCGATCAACGT+TGG	-	chr7.4:5500934-5500953	MS.gene28842:intron	45.0%
	AATCAGCACGGCGAAGGAAT+GGG	-	chr7.4:5500703-5500722	MS.gene28842:intron	50.0%
	AGAAATGGGTCCACTTTCGG+TGG	-	chr7.4:5500737-5500756	MS.gene28842:intron	50.0%
	GTCAAGTTCACCACCGAAAG+TGG	+	chr7.4:5500750-5500769	None:intergenic	50.0%
	GAATCAGCACGGCGAAGGAA+TGG	-	chr7.4:5500702-5500721	MS.gene28842:intron	55.0%
	GTACAGAATCAGCACGGCGA+AGG	-	chr7.4:5500697-5500716	MS.gene28842:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	5500683	5501113	5500683	ID=MS.gene28842
chr7.4	mRNA	5500683	5501113	5500683	ID=MS.gene28842.t1;Parent=MS.gene28842
chr7.4	exon	5500988	5501113	5500988	ID=MS.gene28842.t1.exon1;Parent=MS.gene28842.t1
chr7.4	CDS	5500988	5501113	5500988	ID=cds.MS.gene28842.t1;Parent=MS.gene28842.t1
chr7.4	exon	5500781	5500855	5500781	ID=MS.gene28842.t1.exon2;Parent=MS.gene28842.t1
chr7.4	CDS	5500781	5500855	5500781	ID=cds.MS.gene28842.t1;Parent=MS.gene28842.t1
chr7.4	exon	5500683	5500688	5500683	ID=MS.gene28842.t1.exon3;Parent=MS.gene28842.t1
chr7.4	CDS	5500683	5500688	5500683	ID=cds.MS.gene28842.t1;Parent=MS.gene28842.t1

Gene Sequence

>MS.gene28842

ATGAGTGATTTTGTGTACAGAATCAGCACGGCGAAGGAATGGGAAGAATTGCAAAGAAATGGGTCCACTTTCGGTGGTGAACTTGACAAGTCTTCTTCTTTCATCCATCTTAGCAAACTAGATCAGGTTAATTTATTTCTATGTTTTTCTAATATTTTGATTTTGAGTTCATTTCACTGTTTTGCTCATGTTCATATAAGTCGCTTCATTCATTCGGATTTCTGAATTCTGAATTCTATAATTATTTTATTATTGCAGGTTCGATCAACGTTGGATAATTTTTTCTTGAATTCTAAAGATGAACTCTACCTTCTTCAGATTGATGCTAAGAAGGTTTGCTCATACTTCCCTTTCACTATGCTTTTTTTTAATGTGTTTGTGATTTTATTTGTGAATAAATAATTAAAAAACATGCTTATTGGTAGCGATGA

Protein sequence

>MS.gene28842.t1

MSDFVYRISTAKEWEELQRNGSTFGGELDKSSSFIHLSKLDQVRSTLDNFFLNSKDELYLLQIDAKKR