Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32306.t1 | RHN40794.1 | 100 | 207 | 0 | 0 | 1 | 207 | 46 | 252 | 1.10E-95 | 359.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32306.t1 | A0A072TQW7 | 100.0 | 207 | 0 | 0 | 1 | 207 | 1 | 207 | 8.2e-96 | 359.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049251 | MS.gene32306 | 0.841777 | 3.87E-58 | -1.69E-46 |
| MS.gene049596 | MS.gene32306 | 0.834453 | 2.97E-56 | -1.69E-46 |
| MS.gene049989 | MS.gene32306 | 0.811079 | 8.37E-51 | -1.69E-46 |
| MS.gene050297 | MS.gene32306 | 0.817371 | 3.41E-52 | -1.69E-46 |
| MS.gene050875 | MS.gene32306 | 0.815785 | 7.74E-52 | -1.69E-46 |
| MS.gene05124 | MS.gene32306 | 0.839305 | 1.72E-57 | -1.69E-46 |
| MS.gene051914 | MS.gene32306 | 0.842743 | 2.15E-58 | -1.69E-46 |
| MS.gene051955 | MS.gene32306 | 0.851492 | 8.57E-61 | -1.69E-46 |
| MS.gene052143 | MS.gene32306 | 0.857366 | 1.71E-62 | -1.69E-46 |
| MS.gene052308 | MS.gene32306 | 0.861651 | 8.83E-64 | -1.69E-46 |
| MS.gene052367 | MS.gene32306 | 0.873507 | 1.39E-67 | -1.69E-46 |
| MS.gene053173 | MS.gene32306 | 0.879174 | 1.54E-69 | -1.69E-46 |
| MS.gene053348 | MS.gene32306 | 0.822053 | 2.91E-53 | -1.69E-46 |
| MS.gene053416 | MS.gene32306 | 0.827491 | 1.52E-54 | -1.69E-46 |
| MS.gene053417 | MS.gene32306 | 0.828966 | 6.70E-55 | -1.69E-46 |
| MS.gene054551 | MS.gene32306 | 0.89005 | 1.40E-73 | -1.69E-46 |
| MS.gene054552 | MS.gene32306 | 0.813246 | 2.82E-51 | -1.69E-46 |
| MS.gene054593 | MS.gene32306 | 0.866943 | 1.97E-65 | -1.69E-46 |
| MS.gene054633 | MS.gene32306 | 0.854612 | 1.10E-61 | -1.69E-46 |
| MS.gene054643 | MS.gene32306 | 0.820353 | 7.17E-53 | -1.69E-46 |
| MS.gene054662 | MS.gene32306 | 0.830305 | 3.16E-55 | -1.69E-46 |
| MS.gene055308 | MS.gene32306 | 0.840927 | 6.47E-58 | -1.69E-46 |
| MS.gene055578 | MS.gene32306 | 0.850677 | 1.46E-60 | -1.69E-46 |
| MS.gene055957 | MS.gene32306 | 0.831964 | 1.24E-55 | -1.69E-46 |
| MS.gene056041 | MS.gene32306 | 0.845686 | 3.48E-59 | -1.69E-46 |
| MS.gene056266 | MS.gene32306 | -0.815432 | 9.27E-52 | -1.69E-46 |
| MS.gene05640 | MS.gene32306 | 0.851539 | 8.31E-61 | -1.69E-46 |
| MS.gene056526 | MS.gene32306 | 0.810905 | 9.14E-51 | -1.69E-46 |
| MS.gene05732 | MS.gene32306 | 0.80919 | 2.14E-50 | -1.69E-46 |
| MS.gene057367 | MS.gene32306 | 0.81386 | 2.07E-51 | -1.69E-46 |
| MS.gene057458 | MS.gene32306 | 0.836561 | 8.71E-57 | -1.69E-46 |
| MS.gene058128 | MS.gene32306 | 0.815708 | 8.05E-52 | -1.69E-46 |
| MS.gene058818 | MS.gene32306 | 0.836277 | 1.03E-56 | -1.69E-46 |
| MS.gene058844 | MS.gene32306 | 0.822334 | 2.50E-53 | -1.69E-46 |
| MS.gene058853 | MS.gene32306 | 0.855027 | 8.31E-62 | -1.69E-46 |
| MS.gene059553 | MS.gene32306 | 0.801943 | 7.09E-49 | -1.69E-46 |
| MS.gene060473 | MS.gene32306 | 0.838682 | 2.49E-57 | -1.69E-46 |
| MS.gene060565 | MS.gene32306 | 0.831526 | 1.59E-55 | -1.69E-46 |
| MS.gene06093 | MS.gene32306 | 0.857088 | 2.07E-62 | -1.69E-46 |
| MS.gene061099 | MS.gene32306 | 0.842862 | 2.00E-58 | -1.69E-46 |
| MS.gene061260 | MS.gene32306 | 0.865754 | 4.70E-65 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32306.t1 | MTR_8g445730 | 100.000 | 207 | 0 | 0 | 1 | 207 | 1 | 207 | 6.78e-152 | 419 |
| MS.gene32306.t1 | MTR_3g461540 | 37.500 | 192 | 113 | 3 | 23 | 207 | 41 | 232 | 1.70e-24 | 96.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32306.t1 | AT3G12870 | 72.139 | 201 | 52 | 1 | 10 | 206 | 5 | 205 | 1.38e-99 | 287 |
| MS.gene32306.t1 | AT5G56120 | 37.500 | 200 | 118 | 2 | 15 | 207 | 36 | 235 | 1.35e-27 | 104 |
Find 55 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTTCTCTGTTGTTCTCAT+TGG | 0.156426 | 8.3:-49353709 | MS.gene32306:CDS |
| TGTTCTCATTGGGGCTTATA+TGG | 0.200526 | 8.3:-49353698 | MS.gene32306:CDS |
| TGTTCTCTGTTGTTCTCATT+GGG | 0.246862 | 8.3:-49353708 | MS.gene32306:CDS |
| CTTTGCGGATTGAATCTATC+TGG | 0.255908 | 8.3:+49353850 | None:intergenic |
| GTTTGTCTCTTGCTGTCATA+AGG | 0.289653 | 8.3:-49353437 | MS.gene32306:CDS |
| CATGATTTGTGACAAGCTTT+AGG | 0.297093 | 8.3:+49353744 | None:intergenic |
| AAGGGTTATCTTGTGTAGTT+AGG | 0.302967 | 8.3:-49353418 | None:intergenic |
| GAGTTTGTGGGTTGGTGTTT+CGG | 0.319413 | 8.3:+49353935 | None:intergenic |
| GTTCTCATTGGGGCTTATAT+GGG | 0.331811 | 8.3:-49353697 | MS.gene32306:CDS |
| CTTTGAGCCATTGCTTTAAG+AGG | 0.341158 | 8.3:+49353913 | None:intergenic |
| TGGGGCTTATATGGGCTTTG+AGG | 0.363451 | 8.3:-49353689 | MS.gene32306:CDS |
| GGGTTGGTGTTTCGGCAATT+TGG | 0.381774 | 8.3:+49353943 | None:intergenic |
| GAGTTGAAAGTAAAGAAGTT+AGG | 0.394819 | 8.3:-49353509 | MS.gene32306:CDS |
| CTAAAGCTTGTCACAAATCA+TGG | 0.398644 | 8.3:-49353743 | MS.gene32306:CDS |
| TTTGTCTCTTGCTGTCATAA+GGG | 0.402196 | 8.3:-49353436 | MS.gene32306:CDS |
| GAGTGATGTAGAGGCTCATA+TGG | 0.426429 | 8.3:-49353661 | MS.gene32306:CDS |
| GGAGCATGAGGGTAGTTGAA+TGG | 0.435925 | 8.3:+49353793 | None:intergenic |
| GTTGGAGTTTGATTTGTTGA+AGG | 0.447000 | 8.3:-49353565 | MS.gene32306:CDS |
| TGAGGGTAGTTGAATGGAAG+AGG | 0.447303 | 8.3:+49353799 | None:intergenic |
| AAAGTAAAGAAGTTAGGAAA+TGG | 0.454954 | 8.3:-49353503 | MS.gene32306:CDS |
| TTTGCGGATTGAATCTATCT+GGG | 0.463339 | 8.3:+49353851 | None:intergenic |
| TTGCTTTAAGAGGAGTTTGT+GGG | 0.465955 | 8.3:+49353923 | None:intergenic |
| AGGGTTATCTTGTGTAGTTA+GGG | 0.471643 | 8.3:-49353417 | None:intergenic |
| AAGAGTTGAAGAGGAGCATG+AGG | 0.477929 | 8.3:+49353781 | None:intergenic |
| GATGTTGTCGAGGGAGAAAG+AGG | 0.486683 | 8.3:-49353634 | MS.gene32306:CDS |
| AAGTTAGGAAATGGTCTTCA+CGG | 0.486784 | 8.3:-49353494 | MS.gene32306:CDS |
| AAAGAAGAAGAACTCATCAA+TGG | 0.502941 | 8.3:-49353894 | MS.gene32306:CDS |
| AAGAGGATCGTGGTTTGTTG+AGG | 0.507711 | 8.3:-49353617 | MS.gene32306:CDS |
| GTGGAGGAATTGAAGAAGAA+AGG | 0.509827 | 8.3:-49353588 | MS.gene32306:CDS |
| CAGTGTAAGATCTTCAATCA+TGG | 0.516363 | 8.3:-49353991 | MS.gene32306:CDS |
| AGAGCATTGTGATTTCTTTG+CGG | 0.523269 | 8.3:+49353835 | None:intergenic |
| AGTTAGGAAATGGTCTTCAC+GGG | 0.529008 | 8.3:-49353493 | MS.gene32306:CDS |
| GTTCTCTGTTGTTCTCATTG+GGG | 0.532664 | 8.3:-49353707 | MS.gene32306:CDS |
| GTTTCGGCAATTTGGTGAAG+TGG | 0.538425 | 8.3:+49353951 | None:intergenic |
| GGAATTGAAGAAGAAAGGGT+TGG | 0.541831 | 8.3:-49353583 | MS.gene32306:CDS |
| TTTCGGCAATTTGGTGAAGT+GGG | 0.543957 | 8.3:+49353952 | None:intergenic |
| ATTGCTTTAAGAGGAGTTTG+TGG | 0.546009 | 8.3:+49353922 | None:intergenic |
| TGGAGGAATTGAAGAAGAAA+GGG | 0.548993 | 8.3:-49353587 | MS.gene32306:CDS |
| TAAGATCTTCAATCATGGAA+TGG | 0.557240 | 8.3:-49353986 | MS.gene32306:CDS |
| AGAGTTGAAGAGGAGCATGA+GGG | 0.559380 | 8.3:+49353782 | None:intergenic |
| TAACAGAGGAAGAGTTGAAG+AGG | 0.573029 | 8.3:+49353772 | None:intergenic |
| AACAACAGAGAACAGAGTGA+TGG | 0.574344 | 8.3:+49353717 | None:intergenic |
| AGGAAGTTGATGCTTTGAGA+AGG | 0.575406 | 8.3:-49353545 | MS.gene32306:CDS |
| ACAACAGAGAACAGAGTGAT+GGG | 0.581993 | 8.3:+49353718 | None:intergenic |
| GGAAGTTGATGCTTTGAGAA+GGG | 0.582663 | 8.3:-49353544 | MS.gene32306:CDS |
| TTTAAGAGGAGTTTGTGGGT+TGG | 0.603717 | 8.3:+49353927 | None:intergenic |
| GAGGTATAAGAGTGATGTAG+AGG | 0.608995 | 8.3:-49353670 | MS.gene32306:CDS |
| TAAAGCTTGTCACAAATCAT+GGG | 0.609433 | 8.3:-49353742 | MS.gene32306:CDS |
| ACAAACTCCTCTTAAAGCAA+TGG | 0.614010 | 8.3:-49353920 | MS.gene32306:CDS |
| AGCTTTAGGTGTTGTAACAG+AGG | 0.622557 | 8.3:+49353758 | None:intergenic |
| ATATGGAGAAGATGTTGTCG+AGG | 0.640413 | 8.3:-49353644 | MS.gene32306:CDS |
| TATGGAGAAGATGTTGTCGA+GGG | 0.649748 | 8.3:-49353643 | MS.gene32306:CDS |
| TGGTTTGTTGAGGAAATGCG+TGG | 0.673610 | 8.3:-49353607 | MS.gene32306:CDS |
| TCGAGGGAGAAAGAGGATCG+TGG | 0.688204 | 8.3:-49353627 | MS.gene32306:CDS |
| TTTGTTGAGGAAATGCGTGG+AGG | 0.708375 | 8.3:-49353604 | MS.gene32306:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGTAAAGAAGTTAGGAAA+TGG | - | chr8.3:49353936-49353955 | MS.gene32306:CDS | 25.0% |
| AAAGAAGAAGAACTCATCAA+TGG | - | chr8.3:49353545-49353564 | MS.gene32306:CDS | 30.0% | |
| GAGTTGAAAGTAAAGAAGTT+AGG | - | chr8.3:49353930-49353949 | MS.gene32306:CDS | 30.0% | |
| TAAAGCTTGTCACAAATCAT+GGG | - | chr8.3:49353697-49353716 | MS.gene32306:CDS | 30.0% | |
| TAAGATCTTCAATCATGGAA+TGG | - | chr8.3:49353453-49353472 | MS.gene32306:CDS | 30.0% | |
| !! | TTCTCTGTTTTTCTTCTCAA+TGG | - | chr8.3:49353976-49353995 | MS.gene32306:CDS | 30.0% |
| AAGTTAGGAAATGGTCTTCA+CGG | - | chr8.3:49353945-49353964 | MS.gene32306:CDS | 35.0% | |
| ACAAACTCCTCTTAAAGCAA+TGG | - | chr8.3:49353519-49353538 | MS.gene32306:CDS | 35.0% | |
| CAGTGTAAGATCTTCAATCA+TGG | - | chr8.3:49353448-49353467 | MS.gene32306:CDS | 35.0% | |
| CTAAAGCTTGTCACAAATCA+TGG | - | chr8.3:49353696-49353715 | MS.gene32306:CDS | 35.0% | |
| TGGAGGAATTGAAGAAGAAA+GGG | - | chr8.3:49353852-49353871 | MS.gene32306:CDS | 35.0% | |
| TGTTCTCTGTTGTTCTCATT+GGG | - | chr8.3:49353731-49353750 | MS.gene32306:CDS | 35.0% | |
| ! | CATGATTTGTGACAAGCTTT+AGG | + | chr8.3:49353698-49353717 | None:intergenic | 35.0% |
| ! | TTTGCGGATTGAATCTATCT+GGG | + | chr8.3:49353591-49353610 | None:intergenic | 35.0% |
| ! | TTTGTCTCTTGCTGTCATAA+GGG | - | chr8.3:49354003-49354022 | MS.gene32306:CDS | 35.0% |
| !! | AGAGCATTGTGATTTCTTTG+CGG | + | chr8.3:49353607-49353626 | None:intergenic | 35.0% |
| !! | ATTGCTTTAAGAGGAGTTTG+TGG | + | chr8.3:49353520-49353539 | None:intergenic | 35.0% |
| !! | GTTGGAGTTTGATTTGTTGA+AGG | - | chr8.3:49353874-49353893 | MS.gene32306:CDS | 35.0% |
| !! | TTGCTTTAAGAGGAGTTTGT+GGG | + | chr8.3:49353519-49353538 | None:intergenic | 35.0% |
| AACAACAGAGAACAGAGTGA+TGG | + | chr8.3:49353725-49353744 | None:intergenic | 40.0% | |
| ACAACAGAGAACAGAGTGAT+GGG | + | chr8.3:49353724-49353743 | None:intergenic | 40.0% | |
| AGTTAGGAAATGGTCTTCAC+GGG | - | chr8.3:49353946-49353965 | MS.gene32306:CDS | 40.0% | |
| ATATGGAGAAGATGTTGTCG+AGG | - | chr8.3:49353795-49353814 | MS.gene32306:CDS | 40.0% | |
| CTGTTCTCTGTTGTTCTCAT+TGG | - | chr8.3:49353730-49353749 | MS.gene32306:CDS | 40.0% | |
| GAGGTATAAGAGTGATGTAG+AGG | - | chr8.3:49353769-49353788 | MS.gene32306:CDS | 40.0% | |
| GGAATTGAAGAAGAAAGGGT+TGG | - | chr8.3:49353856-49353875 | MS.gene32306:CDS | 40.0% | |
| GTGGAGGAATTGAAGAAGAA+AGG | - | chr8.3:49353851-49353870 | MS.gene32306:CDS | 40.0% | |
| GTTCTCATTGGGGCTTATAT+GGG | - | chr8.3:49353742-49353761 | MS.gene32306:CDS | 40.0% | |
| GTTCTCTGTTGTTCTCATTG+GGG | - | chr8.3:49353732-49353751 | MS.gene32306:CDS | 40.0% | |
| TAACAGAGGAAGAGTTGAAG+AGG | + | chr8.3:49353670-49353689 | None:intergenic | 40.0% | |
| TATGGAGAAGATGTTGTCGA+GGG | - | chr8.3:49353796-49353815 | MS.gene32306:CDS | 40.0% | |
| TGTTCTCATTGGGGCTTATA+TGG | - | chr8.3:49353741-49353760 | MS.gene32306:CDS | 40.0% | |
| TTTAAGAGGAGTTTGTGGGT+TGG | + | chr8.3:49353515-49353534 | None:intergenic | 40.0% | |
| ! | CTTTGAGCCATTGCTTTAAG+AGG | + | chr8.3:49353529-49353548 | None:intergenic | 40.0% |
| ! | CTTTGCGGATTGAATCTATC+TGG | + | chr8.3:49353592-49353611 | None:intergenic | 40.0% |
| ! | GTTTGTCTCTTGCTGTCATA+AGG | - | chr8.3:49354002-49354021 | MS.gene32306:CDS | 40.0% |
| ! | TTTCGGCAATTTGGTGAAGT+GGG | + | chr8.3:49353490-49353509 | None:intergenic | 40.0% |
| !! | AGCTTTAGGTGTTGTAACAG+AGG | + | chr8.3:49353684-49353703 | None:intergenic | 40.0% |
| !! | AGGAAGTTGATGCTTTGAGA+AGG | - | chr8.3:49353894-49353913 | MS.gene32306:CDS | 40.0% |
| !! | GGAAGTTGATGCTTTGAGAA+GGG | - | chr8.3:49353895-49353914 | MS.gene32306:CDS | 40.0% |
| GAGTGATGTAGAGGCTCATA+TGG | - | chr8.3:49353778-49353797 | MS.gene32306:CDS | 45.0% | |
| TGAGGGTAGTTGAATGGAAG+AGG | + | chr8.3:49353643-49353662 | None:intergenic | 45.0% | |
| TTTGTTGAGGAAATGCGTGG+AGG | - | chr8.3:49353835-49353854 | MS.gene32306:CDS | 45.0% | |
| ! | AAGAGTTGAAGAGGAGCATG+AGG | + | chr8.3:49353661-49353680 | None:intergenic | 45.0% |
| ! | AGAGTTGAAGAGGAGCATGA+GGG | + | chr8.3:49353660-49353679 | None:intergenic | 45.0% |
| ! | GTTTCGGCAATTTGGTGAAG+TGG | + | chr8.3:49353491-49353510 | None:intergenic | 45.0% |
| !! | AAGAGGATCGTGGTTTGTTG+AGG | - | chr8.3:49353822-49353841 | MS.gene32306:CDS | 45.0% |
| !! | GAGTTTGTGGGTTGGTGTTT+CGG | + | chr8.3:49353507-49353526 | None:intergenic | 45.0% |
| !! | TGGTTTGTTGAGGAAATGCG+TGG | - | chr8.3:49353832-49353851 | MS.gene32306:CDS | 45.0% |
| GATGTTGTCGAGGGAGAAAG+AGG | - | chr8.3:49353805-49353824 | MS.gene32306:CDS | 50.0% | |
| GGAGCATGAGGGTAGTTGAA+TGG | + | chr8.3:49353649-49353668 | None:intergenic | 50.0% | |
| TGGGGCTTATATGGGCTTTG+AGG | - | chr8.3:49353750-49353769 | MS.gene32306:CDS | 50.0% | |
| !! | GGGTTGGTGTTTCGGCAATT+TGG | + | chr8.3:49353499-49353518 | None:intergenic | 50.0% |
| TCGAGGGAGAAAGAGGATCG+TGG | - | chr8.3:49353812-49353831 | MS.gene32306:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 49353419 | 49354042 | 49353419 | ID=MS.gene32306 |
| chr8.3 | mRNA | 49353419 | 49354042 | 49353419 | ID=MS.gene32306.t1;Parent=MS.gene32306 |
| chr8.3 | exon | 49353419 | 49354042 | 49353419 | ID=MS.gene32306.t1.exon1;Parent=MS.gene32306.t1 |
| chr8.3 | CDS | 49353419 | 49354042 | 49353419 | ID=cds.MS.gene32306.t1;Parent=MS.gene32306.t1 |
| Gene Sequence |
| Protein sequence |
