AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35433


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35433.t1 MTR_2g086740 96.818 220 7 0 1 220 1 220 2.13e-157 434
MS.gene35433.t1 MTR_2g086740 96.667 150 5 0 1 150 1 150 8.28e-106 301
MS.gene35433.t1 MTR_1g058540 59.064 171 70 0 49 219 25 195 4.12e-61 190
MS.gene35433.t1 MTR_1g058560 59.538 173 70 0 47 219 22 194 2.04e-57 180
MS.gene35433.t1 MTR_1g058570 56.725 171 74 0 49 219 23 193 3.29e-55 175
MS.gene35433.t1 MTR_7g011090 48.276 174 90 0 46 219 12 185 1.41e-50 162
MS.gene35433.t1 MTR_4g081880 43.243 185 99 1 42 220 3 187 1.08e-46 153
MS.gene35433.t1 MTR_5g041900 43.590 195 100 1 26 220 2 186 4.12e-42 141
MS.gene35433.t1 MTR_7g083120 33.094 139 90 2 65 202 33 169 3.80e-16 73.9
MS.gene35433.t1 MTR_0004s0210 34.899 149 84 4 55 201 1 138 6.16e-16 72.8
MS.gene35433.t1 MTR_7g108130 28.877 187 115 5 24 206 3 175 4.40e-15 71.2
MS.gene35433.t1 MTR_3g064310 28.141 199 103 5 40 216 7 187 3.36e-14 69.3
MS.gene35433.t1 MTR_0097s0070 29.565 115 81 0 99 213 71 185 4.90e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35433.t1 AT2G27370 49.091 220 103 4 3 220 8 220 3.56e-64 198
MS.gene35433.t1 AT2G36100 54.762 168 76 0 52 219 38 205 9.44e-59 184
MS.gene35433.t1 AT5G06200 60.479 167 66 0 53 219 35 201 3.92e-54 172
MS.gene35433.t1 AT5G15290 49.686 159 79 1 43 200 8 166 1.53e-51 165
MS.gene35433.t1 AT3G11550 59.880 167 67 0 53 219 37 203 6.86e-50 161
MS.gene35433.t1 AT1G14160 43.030 165 94 0 55 219 44 208 2.74e-42 142
MS.gene35433.t1 AT1G17200 29.193 161 105 3 58 217 29 181 2.77e-15 72.0
MS.gene35433.t1 AT3G06390 28.161 174 104 4 24 190 11 170 3.28e-15 71.6
MS.gene35433.t1 AT5G44550 30.247 162 101 4 66 219 22 179 5.97e-12 62.8
MS.gene35433.t1 AT1G03700 32.000 150 80 5 65 206 12 147 2.96e-11 60.1
MS.gene35433.t1 AT4G15610 27.545 167 117 2 40 202 7 173 5.35e-11 60.1

Find 48 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTGTTCTCTTGGATTCT+AGG 0.131201 2.1:+15485475 None:intergenic
TCTTGGTGCTTCTGTCATTA+TGG 0.177988 2.1:-15485298 MS.gene35433:CDS
TACCTAATTGCAGGTATTAA+TGG 0.240857 2.1:-15484390 MS.gene35433:intron
TCCATTGCTTCTTGTTCTCT+TGG 0.247900 2.1:+15485467 None:intergenic
CTTGGTGCTTCTGTCATTAT+GGG 0.276810 2.1:-15485297 MS.gene35433:CDS
AAATCTACTCCAATCCAAAA+AGG 0.350579 2.1:-15485384 MS.gene35433:CDS
TCCTTCAGTTTCATGCTCAA+TGG 0.391797 2.1:-15485239 MS.gene35433:CDS
CCTTCAGTTTCATGCTCAAT+GGG 0.394063 2.1:-15485238 MS.gene35433:CDS
GGTGCCATTGGTGCTACTCT+TGG 0.400369 2.1:-15485315 MS.gene35433:CDS
TGTTCTCTTGGATTCTAGGA+TGG 0.406134 2.1:+15485479 None:intergenic
GCAGGTATTAATGGCCTTAG+TGG 0.406928 2.1:-15484381 MS.gene35433:intron
ACATGAACTTACTTAAACAT+TGG 0.425523 2.1:+15485207 None:intergenic
AGAACAAGAAGCAATGGAAA+AGG 0.427010 2.1:-15485462 MS.gene35433:CDS
ACACCACAACCAAACAAGAT+AGG 0.440488 2.1:+15484210 None:intergenic
ATGGGAGTCAAGATGCAAAC+TGG 0.445257 2.1:-15484313 MS.gene35433:CDS
GTTCTCTTGGATTCTAGGAT+GGG 0.446748 2.1:+15485480 None:intergenic
CTAAACATGCAGGTTCTTTG+TGG 0.448810 2.1:-15484634 MS.gene35433:intron
GAACAAGAAGCAATGGAAAA+GGG 0.449907 2.1:-15485461 MS.gene35433:CDS
ATTGTTAGACCCCTTGCAGC+TGG 0.465698 2.1:-15484552 MS.gene35433:CDS
GGAAAAGGGAAATCCATTGA+TGG 0.488181 2.1:-15485447 MS.gene35433:CDS
TTTCCTATCTTGTTTGGTTG+TGG 0.489463 2.1:-15484213 MS.gene35433:CDS
CCAAGGTTCAAGTTTGGCTG+TGG 0.491266 2.1:-15484258 MS.gene35433:CDS
GTGGCAAATGGAGCAGCAGC+TGG 0.512854 2.1:-15484612 MS.gene35433:CDS
TTGTGTACTTAGCACACAAT+GGG 0.520027 2.1:-15484331 MS.gene35433:CDS
TGTCTTCTGTAGCTCTCAAA+AGG 0.525875 2.1:-15484190 MS.gene35433:CDS
AGGTTCTTTGTGGTGGCAAA+TGG 0.527477 2.1:-15484624 MS.gene35433:CDS
GACCCCTTGCAGCTGGTCCA+CGG 0.529518 2.1:-15484545 MS.gene35433:CDS
TCCAAGAGAACAAGAAGCAA+TGG 0.530743 2.1:-15485468 MS.gene35433:CDS
CCACAGCCAAACTTGAACCT+TGG 0.532217 2.1:+15484258 None:intergenic
AGGAGAATGATCTCCATCAA+TGG 0.544643 2.1:+15485434 None:intergenic
TGAAGCAGCTGCAACCACTA+AGG 0.546688 2.1:+15484367 None:intergenic
TGAAGGAACTGAGTAAAGAA+AGG 0.555422 2.1:+15485255 None:intergenic
AATCCGTGGACCAGCTGCAA+GGG 0.560165 2.1:+15484542 None:intergenic
AACATGCAGGTTCTTTGTGG+TGG 0.566783 2.1:-15484631 MS.gene35433:intron
GGACTCAAGAAGAGAAGTTG+AGG 0.570390 2.1:-15485517 MS.gene35433:CDS
TTGGTGCTTCTGTCATTATG+GGG 0.574068 2.1:-15485296 MS.gene35433:CDS
GCAGACAATTGAGAATGGTA+AGG 0.574191 2.1:+15484575 None:intergenic
GGTTGTTTCTTTCGTCGCGT+CGG 0.575059 2.1:-15484237 MS.gene35433:CDS
ACAATGCAGACAATTGAGAA+TGG 0.577153 2.1:+15484570 None:intergenic
CCCATTGAGCATGAAACTGA+AGG 0.601992 2.1:+15485238 None:intergenic
GAATCCGTGGACCAGCTGCA+AGG 0.605753 2.1:+15484541 None:intergenic
AGCACCAAGAGTAGCACCAA+TGG 0.630653 2.1:+15485311 None:intergenic
GTTGTGTACTTAGCACACAA+TGG 0.633961 2.1:-15484332 MS.gene35433:CDS
ATCCGTGGACCAGCTGCAAG+GGG 0.635198 2.1:+15484543 None:intergenic
CAATTGAGAATGGTAAGGAG+AGG 0.647713 2.1:+15484580 None:intergenic
ACAACAGTTGCAGCATGTGG+AGG 0.650553 2.1:+15485414 None:intergenic
GTAACAACAGTTGCAGCATG+TGG 0.663640 2.1:+15485411 None:intergenic
ACGATGACAAGTAGAATCCG+TGG 0.676654 2.1:+15484528 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTACAATTAATTGTATTTT+TGG - chr2.1:15484618-15484637 MS.gene35433:CDS 10.0%
!! AAAAAAAAAATGTTCTTAGA+GGG - chr2.1:15484862-15484881 MS.gene35433:intron 15.0%
!! AAACAATAAATTTCAAGAAT+AGG - chr2.1:15484812-15484831 MS.gene35433:intron 15.0%
!!! TTTAAATATCATCCTTTTTA+TGG - chr2.1:15485044-15485063 MS.gene35433:intron 15.0%
!! AATTGTGTTTCAGTTAAAAT+AGG + chr2.1:15484648-15484667 None:intergenic 20.0%
!! ATAGTATTGTAAAGATCAAA+TGG + chr2.1:15484539-15484558 None:intergenic 20.0%
!! GAAAAAAAAAATGTTCTTAG+AGG - chr2.1:15484861-15484880 MS.gene35433:intron 20.0%
!! TAGTATTGTAAAGATCAAAT+GGG + chr2.1:15484538-15484557 None:intergenic 20.0%
!!! AGAAAAAAGTTTTGAAACAA+GGG + chr2.1:15484705-15484724 None:intergenic 20.0%
! ACATGAACTTACTTAAACAT+TGG + chr2.1:15484499-15484518 None:intergenic 25.0%
! AGTAAGTTCATGTTACATAA+CGG - chr2.1:15484506-15484525 MS.gene35433:intron 25.0%
! TTACATCCGATAAATGAAAT+CGG - chr2.1:15484836-15484855 MS.gene35433:intron 25.0%
! TTTCTTCCGATTTCATTTAT+CGG + chr2.1:15484845-15484864 None:intergenic 25.0%
!! CAATCTTAGACTTCATTTTA+AGG - chr2.1:15484362-15484381 MS.gene35433:CDS 25.0%
!!! AACACTTTTTAAGAATGACT+TGG - chr2.1:15484587-15484606 MS.gene35433:CDS 25.0%
!!! GAAAAAAGTTTTGAAACAAG+GGG + chr2.1:15484704-15484723 None:intergenic 25.0%
!!! GAGAAAAAAGTTTTGAAACA+AGG + chr2.1:15484706-15484725 None:intergenic 25.0%
!!! TTTTCTCACAACTTAATTTG+AGG - chr2.1:15484719-15484738 MS.gene35433:intron 25.0%
AAATCTACTCCAATCCAAAA+AGG - chr2.1:15484319-15484338 MS.gene35433:CDS 30.0%
AACATGTCACGTCATAAAAT+AGG + chr2.1:15484748-15484767 None:intergenic 30.0%
CAAAAAGGTGGAATGAAAAA+GGG - chr2.1:15484334-15484353 MS.gene35433:CDS 30.0%
GCATGTTTAGATCCATAAAA+AGG + chr2.1:15485059-15485078 None:intergenic 30.0%
TACCTAATTGCAGGTATTAA+TGG - chr2.1:15485313-15485332 MS.gene35433:CDS 30.0%
TGTGTTTCAGTTAAAATAGG+AGG + chr2.1:15484645-15484664 None:intergenic 30.0%
! TTTTATGGATCTAAACATGC+AGG - chr2.1:15485059-15485078 MS.gene35433:intron 30.0%
!! ACGATATGAGAGTGTTTATT+TGG + chr2.1:15484894-15484913 None:intergenic 30.0%
!!! TAAACAATGTTTTGATGACG+TGG + chr2.1:15484927-15484946 None:intergenic 30.0%
!!! TTAGACTTCATTTTAAGGCT+TGG - chr2.1:15484367-15484386 MS.gene35433:CDS 30.0%
ACAATGCAGACAATTGAGAA+TGG + chr2.1:15485136-15485155 None:intergenic 35.0%
AGAACAAGAAGCAATGGAAA+AGG - chr2.1:15484241-15484260 MS.gene35433:CDS 35.0%
CCAAAAAGGTGGAATGAAAA+AGG - chr2.1:15484333-15484352 MS.gene35433:CDS 35.0%
CGAAATTGCAAGGATATGAA+GGG + chr2.1:15485000-15485019 None:intergenic 35.0%
GAACAAGAAGCAATGGAAAA+GGG - chr2.1:15484242-15484261 MS.gene35433:CDS 35.0%
TGAAGGAACTGAGTAAAGAA+AGG + chr2.1:15484451-15484470 None:intergenic 35.0%
TTCTTGTTCTCTTGGATTCT+AGG + chr2.1:15484231-15484250 None:intergenic 35.0%
TTGTGTACTTAGCACACAAT+GGG - chr2.1:15485372-15485391 MS.gene35433:CDS 35.0%
! TTCATTCCACCTTTTTGGAT+TGG + chr2.1:15484331-15484350 None:intergenic 35.0%
! TTTCCTATCTTGTTTGGTTG+TGG - chr2.1:15485490-15485509 MS.gene35433:CDS 35.0%
!! CCTTTTTCATTCCACCTTTT+TGG + chr2.1:15484336-15484355 None:intergenic 35.0%
!! TTTTTGCCAAGGTTCAAGTT+TGG - chr2.1:15485439-15485458 MS.gene35433:CDS 35.0%
AATGTTCGCTACCTAATTGC+AGG - chr2.1:15485304-15485323 MS.gene35433:CDS 40.0%
AATTGCAAGGATATGAAGGG+AGG + chr2.1:15484997-15485016 None:intergenic 40.0%
ACACCACAACCAAACAAGAT+AGG + chr2.1:15485496-15485515 None:intergenic 40.0%
AGGAGAATGATCTCCATCAA+TGG + chr2.1:15484272-15484291 None:intergenic 40.0%
CAATTGAGAATGGTAAGGAG+AGG + chr2.1:15485126-15485145 None:intergenic 40.0%
CCTTCAGTTTCATGCTCAAT+GGG - chr2.1:15484465-15484484 MS.gene35433:intron 40.0%
CTAAACATGCAGGTTCTTTG+TGG - chr2.1:15485069-15485088 MS.gene35433:intron 40.0%
CTATAGCAAGTAAAGATGCC+AGG + chr2.1:15485023-15485042 None:intergenic 40.0%
GCAGACAATTGAGAATGGTA+AGG + chr2.1:15485131-15485150 None:intergenic 40.0%
GCGAAATTGCAAGGATATGA+AGG + chr2.1:15485001-15485020 None:intergenic 40.0%
GGAAAAGGGAAATCCATTGA+TGG - chr2.1:15484256-15484275 MS.gene35433:CDS 40.0%
GGCCATTAATACCTGCAATT+AGG + chr2.1:15485318-15485337 None:intergenic 40.0%
GTTGTGTACTTAGCACACAA+TGG - chr2.1:15485371-15485390 MS.gene35433:CDS 40.0%
TCCAAGAGAACAAGAAGCAA+TGG - chr2.1:15484235-15484254 MS.gene35433:CDS 40.0%
TCCATTGCTTCTTGTTCTCT+TGG + chr2.1:15484239-15484258 None:intergenic 40.0%
TCCTTCAGTTTCATGCTCAA+TGG - chr2.1:15484464-15484483 MS.gene35433:intron 40.0%
TCTACTCCAATCCAAAAAGG+TGG - chr2.1:15484322-15484341 MS.gene35433:CDS 40.0%
TGTCTTCTGTAGCTCTCAAA+AGG - chr2.1:15485513-15485532 MS.gene35433:CDS 40.0%
! TGTTCTCTTGGATTCTAGGA+TGG + chr2.1:15484227-15484246 None:intergenic 40.0%
! TTGGTGCTTCTGTCATTATG+GGG - chr2.1:15484407-15484426 MS.gene35433:intron 40.0%
!! CTTGGTGCTTCTGTCATTAT+GGG - chr2.1:15484406-15484425 MS.gene35433:intron 40.0%
!! GTCGGTTTTCCTATCTTGTT+TGG - chr2.1:15485484-15485503 MS.gene35433:CDS 40.0%
!! GTTCTCTTGGATTCTAGGAT+GGG + chr2.1:15484226-15484245 None:intergenic 40.0%
!! TCTTGGTGCTTCTGTCATTA+TGG - chr2.1:15484405-15484424 MS.gene35433:intron 40.0%
!!! ATTTTAAGGCTTGGTGCCAT+TGG - chr2.1:15484376-15484395 MS.gene35433:CDS 40.0%
!!! CAGTTCACTGATTTTTGCCA+AGG - chr2.1:15485428-15485447 MS.gene35433:CDS 40.0%
AACATGCAGGTTCTTTGTGG+TGG - chr2.1:15485072-15485091 MS.gene35433:intron 45.0%
ACGATGACAAGTAGAATCCG+TGG + chr2.1:15485178-15485197 None:intergenic 45.0%
AGGTTCTTTGTGGTGGCAAA+TGG - chr2.1:15485079-15485098 MS.gene35433:intron 45.0%
ATGGGAGTCAAGATGCAAAC+TGG - chr2.1:15485390-15485409 MS.gene35433:CDS 45.0%
CATATCCTTGCAATTTCGCC+TGG - chr2.1:15485002-15485021 MS.gene35433:intron 45.0%
CCCATTGAGCATGAAACTGA+AGG + chr2.1:15484468-15484487 None:intergenic 45.0%
GGACTCAAGAAGAGAAGTTG+AGG - chr2.1:15484186-15484205 MS.gene35433:CDS 45.0%
GTAACAACAGTTGCAGCATG+TGG + chr2.1:15484295-15484314 None:intergenic 45.0%
! GCAGGTATTAATGGCCTTAG+TGG - chr2.1:15485322-15485341 MS.gene35433:CDS 45.0%
ACAACAGTTGCAGCATGTGG+AGG + chr2.1:15484292-15484311 None:intergenic 50.0%
AGATGCCAGGCGAAATTGCA+AGG + chr2.1:15485010-15485029 None:intergenic 50.0%
AGCACCAAGAGTAGCACCAA+TGG + chr2.1:15484395-15484414 None:intergenic 50.0%
ATTGTTAGACCCCTTGCAGC+TGG - chr2.1:15485151-15485170 MS.gene35433:intron 50.0%
CCACAGCCAAACTTGAACCT+TGG + chr2.1:15485448-15485467 None:intergenic 50.0%
TGAAGCAGCTGCAACCACTA+AGG + chr2.1:15485339-15485358 None:intergenic 50.0%
! CCAAGGTTCAAGTTTGGCTG+TGG - chr2.1:15485445-15485464 MS.gene35433:CDS 50.0%
!! GGTTGTTTCTTTCGTCGCGT+CGG - chr2.1:15485466-15485485 MS.gene35433:CDS 50.0%
AATCCGTGGACCAGCTGCAA+GGG + chr2.1:15485164-15485183 None:intergenic 55.0%
!! GGTGCCATTGGTGCTACTCT+TGG - chr2.1:15484388-15484407 MS.gene35433:intron 55.0%
ATCCGTGGACCAGCTGCAAG+GGG + chr2.1:15485163-15485182 None:intergenic 60.0%
GAATCCGTGGACCAGCTGCA+AGG + chr2.1:15485165-15485184 None:intergenic 60.0%
GTGGCAAATGGAGCAGCAGC+TGG - chr2.1:15485091-15485110 MS.gene35433:intron 60.0%
GACCCCTTGCAGCTGGTCCA+CGG - chr2.1:15485158-15485177 MS.gene35433:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 15484184 15485541 15484184 ID=MS.gene35433
chr2.1 mRNA 15484184 15485541 15484184 ID=MS.gene35433.t1;Parent=MS.gene35433
chr2.1 exon 15485219 15485541 15485219 ID=MS.gene35433.t1.exon1;Parent=MS.gene35433.t1
chr2.1 CDS 15485219 15485541 15485219 ID=cds.MS.gene35433.t1;Parent=MS.gene35433.t1
chr2.1 exon 15484521 15484644 15484521 ID=MS.gene35433.t1.exon2;Parent=MS.gene35433.t1
chr2.1 CDS 15484521 15484644 15484521 ID=cds.MS.gene35433.t1;Parent=MS.gene35433.t1
chr2.1 exon 15484184 15484399 15484184 ID=MS.gene35433.t1.exon3;Parent=MS.gene35433.t1
chr2.1 CDS 15484184 15484399 15484184 ID=cds.MS.gene35433.t1;Parent=MS.gene35433.t1
Gene Sequence

>MS.gene35433

ATGGACTCAAGAAGAGAAGTTGAGGAATCATCAACAGCTCCCATCCTAGAATCCAAGAGAACAAGAAGCAATGGAAAAGGGAAATCCATTGATGGAGATCATTCTCCTCCACATGCTGCAACTGTTGTTACAACAAAATCTACTCCAATCCAAAAAGGTGGAATGAAAAAGGGAATTGCAATCTTAGACTTCATTTTAAGGCTTGGTGCCATTGGTGCTACTCTTGGTGCTTCTGTCATTATGGGGACTAATGAACAAATACTTCCTTTCTTTACTCAGTTCCTTCAGTTTCATGCTCAATGGGATGATTTTCCAATGTTTAAGTAAGTTCATGTTACATAACGGTGATATCCCATTTGATCTTTACAATACTATTATACATTTTCAGTTTTATTTTTATAATAACACTTTTTAAGAATGACTTGGTGTACAATTTTACAATTAATTGTATTTTTGGTCCTCCTATTTTAACTGAAACACAATTTTAGACTCTCATTTCTAAATTCAGAATTTTAGTCCCCTTGTTTCAAAACTTTTTTCTCACAACTTAATTTGAGGTCTCCTATTTTATGACGTGACATGTTTATTAATGACGTGAAATTAAATTTGATGACTCGATTTGAATTAGAAACAATAAATTTCAAGAATAGGTTTACATCCGATAAATGAAATCGGAAGAAAAAAAAAATGTTCTTAGAGGGTTCTCTCCAAATAAACACTCTCATATCGTCAAATGTAATCCACGTCATCAAAACATTGTTTAAGATAAAAATAGAGAAATTAAATGTGCATATAATTGTTTTTTTTTTTCCTCCCTTCATATCCTTGCAATTTCGCCTGGCATCTTTACTTGCTATAGATTTAAATATCATCCTTTTTATGGATCTAAACATGCAGGTTCTTTGTGGTGGCAAATGGAGCAGCAGCTGGATTTCTCATCCTCTCCTTACCATTCTCAATTGTCTGCATTGTTAGACCCCTTGCAGCTGGTCCACGGATTCTACTTGTCATCGTTGATTTAGTAAGCAAAAATGCAATATGATTAATGATAATGCACTAATCAATAATCATCATTATATCTTATTTCAAATAATCATTCAAAACTTAACTAGAAAAAAAAAATGTTCGCTACCTAATTGCAGGTATTAATGGCCTTAGTGGTTGCAGCTGCTTCATCTGCTGCAGCTGTTGTGTACTTAGCACACAATGGGAGTCAAGATGCAAACTGGAATGCTATTTGTCAGCAGTTCACTGATTTTTGCCAAGGTTCAAGTTTGGCTGTGGTTGTTTCTTTCGTCGCGTCGGTTTTCCTATCTTGTTTGGTTGTGGTGTCTTCTGTAGCTCTCAAAAGGACTTAA

Protein sequence

>MS.gene35433.t1

MDSRREVEESSTAPILESKRTRSNGKGKSIDGDHSPPHAATVVTTKSTPIQKGGMKKGIAILDFILRLGAIGATLGASVIMGTNEQILPFFTQFLQFHAQWDDFPMFKFFVVANGAAAGFLILSLPFSIVCIVRPLAAGPRILLVIVDLVLMALVVAAASSAAAVVYLAHNGSQDANWNAICQQFTDFCQGSSLAVVVSFVASVFLSCLVVVSSVALKRT