AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38166


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38166.t1 MTR_5g098060 91.255 263 21 2 1 262 1 262 3.27e-138 389
MS.gene38166.t1 MTR_7g101080 40.237 169 90 4 33 195 26 189 1.42e-32 120
MS.gene38166.t1 MTR_2g090765 32.500 240 139 5 31 259 22 249 2.24e-27 106
MS.gene38166.t1 MTR_4g059720 35.789 190 111 4 8 194 8 189 8.19e-25 99.4
MS.gene38166.t1 MTR_4g059840 32.653 245 142 9 28 262 55 286 2.93e-22 93.6
MS.gene38166.t1 MTR_2g093990 36.667 150 90 2 48 194 41 188 4.71e-21 89.4
MS.gene38166.t1 MTR_4g059790 32.456 228 140 8 28 247 55 276 2.27e-20 88.6
MS.gene38166.t1 MTR_4g053380 29.237 236 150 6 28 253 59 287 6.37e-20 87.4
MS.gene38166.t1 MTR_7g075453 33.714 175 110 4 47 219 183 353 6.56e-20 88.6
MS.gene38166.t1 MTR_8g087470 32.086 187 106 5 22 190 56 239 6.47e-19 84.7
MS.gene38166.t1 MTR_8g087460 31.551 187 107 5 22 190 56 239 2.39e-18 83.2
MS.gene38166.t1 MTR_2g017970 29.032 217 140 6 48 260 68 274 2.52e-18 82.4
MS.gene38166.t1 MTR_2g017950 28.829 222 124 7 1 190 2 221 2.57e-18 82.8
MS.gene38166.t1 MTR_2g017960 29.381 194 128 4 48 237 68 256 4.25e-18 82.0
MS.gene38166.t1 MTR_8g087450 31.098 164 104 4 31 190 63 221 1.05e-17 80.9
MS.gene38166.t1 MTR_2g437700 34.395 157 96 3 46 197 193 347 1.72e-17 81.6
MS.gene38166.t1 MTR_2g093980 32.961 179 111 5 23 196 45 219 1.05e-16 78.2
MS.gene38166.t1 MTR_4g059780 29.843 191 112 4 26 196 52 240 6.21e-16 76.3
MS.gene38166.t1 MTR_2g093970 28.502 207 121 5 9 190 9 213 1.69e-15 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38166.t1 AT2G04780 65.079 189 66 0 47 235 45 233 2.43e-77 235
MS.gene38166.t1 AT2G04780 65.079 189 66 0 47 235 45 233 2.43e-77 235
MS.gene38166.t1 AT5G44130 33.503 197 119 4 47 237 35 225 6.84e-32 118
MS.gene38166.t1 AT2G20520 36.444 225 125 6 16 224 1 223 5.10e-31 115
MS.gene38166.t1 AT1G03870 33.023 215 135 5 47 258 38 246 1.20e-30 114
MS.gene38166.t1 AT5G60490 40.268 149 87 1 48 194 39 187 6.60e-29 110
MS.gene38166.t1 AT5G03170 36.842 171 104 3 37 204 26 195 1.07e-28 109
MS.gene38166.t1 AT3G46550 34.184 196 114 7 22 211 187 373 9.16e-21 91.3
MS.gene38166.t1 AT3G60900 34.659 176 105 5 39 208 180 351 1.70e-18 84.7
MS.gene38166.t1 AT2G45470 32.889 225 133 5 29 240 170 389 1.34e-16 79.3

Find 68 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTGTCAGCTATGGAATT+TGG 0.170303 5.3:+79281855 MS.gene38166:CDS
CAGTGTTGTTTGCTTGGTTT+TGG 0.201552 5.3:-79281361 None:intergenic
TTGAATTTCACCGATAATTC+TGG 0.232404 5.3:+79281581 MS.gene38166:CDS
TTGCTTCCTGAGGCAATATT+TGG 0.238748 5.3:+79281698 MS.gene38166:CDS
CCTTCAATCCACAAAAGTTT+TGG 0.254005 5.3:+79281331 MS.gene38166:CDS
CTTCTTCAGTGTTGTTTGCT+TGG 0.268289 5.3:-79281367 None:intergenic
AGAGGGTGGACTTACGGTTT+TGG 0.270515 5.3:-79281201 None:intergenic
GATTGTCAGCTATGGAATTT+GGG 0.295753 5.3:+79281856 MS.gene38166:CDS
GGAACTGTCAAAATTAATTC+AGG 0.296860 5.3:+79281602 MS.gene38166:CDS
GATGCGAAGGAACTGTCTTT+TGG 0.299960 5.3:-79281407 None:intergenic
CTCCCAGCACTTTGGTCAGC+TGG 0.303486 5.3:-79281785 None:intergenic
AACCTTGTGTTGGCAACATT+TGG 0.304302 5.3:+79281881 MS.gene38166:CDS
CACTGATATTCCACCTGTTC+CGG 0.305792 5.3:+79281721 MS.gene38166:CDS
GGAGCCGGAGTTGGACTCTC+GGG 0.338226 5.3:-79281227 None:intergenic
TTTGACAGTTCCAGAATTAT+CGG 0.339576 5.3:-79281591 None:intergenic
GTTGGCAACATTTGGTTTGT+TGG 0.348928 5.3:+79281889 MS.gene38166:CDS
TCAGCTGCAGGCGCAATATC+TGG 0.356724 5.3:-79281758 None:intergenic
AATCTCATCAGCTTTGAGTT+TGG 0.359351 5.3:-79281450 None:intergenic
GCCTCAACTCCAACATTTGC+CGG 0.365489 5.3:+79281545 MS.gene38166:CDS
GTGGGTGCAGGAGCCGGAAC+AGG 0.378032 5.3:-79281734 None:intergenic
ACTGAATTACTCACTGTTGC+TGG 0.384658 5.3:+79281284 MS.gene38166:CDS
TCAGTGAGGTTAACAAAATC+TGG 0.394471 5.3:-79281266 None:intergenic
TGCAATTTATCAAGTTGATA+AGG 0.410347 5.3:+79281673 MS.gene38166:CDS
TCAACTTGATAAATTGCAAC+AGG 0.410462 5.3:-79281668 None:intergenic
TTGTAAGAAGACGAACTATC+TGG 0.411721 5.3:-79281833 None:intergenic
TGGAGCCGGAGTTGGACTCT+CGG 0.431321 5.3:-79281228 None:intergenic
ATGTTGGAGTTGAGGCAGTT+TGG 0.434404 5.3:-79281538 None:intergenic
GCTGGTGCTGGAGCCGGAGT+TGG 0.434583 5.3:-79281236 None:intergenic
TATTGAAGAAAAGTGTGAAA+TGG 0.451994 5.3:-79281308 None:intergenic
GTAGGTGCTGGTGCTGGAGC+CGG 0.452561 5.3:-79281242 None:intergenic
GCAATATCTGGTGTGGGTGC+AGG 0.465564 5.3:-79281746 None:intergenic
GAGTTGAGGCAGTTTGGCTA+AGG 0.468259 5.3:-79281532 None:intergenic
TCTCCAGCTGACCAAAGTGC+TGG 0.473142 5.3:+79281782 MS.gene38166:CDS
TCTGGTGTGGGTGCAGGAGC+CGG 0.477035 5.3:-79281740 None:intergenic
GAGCCGGAGTTGGACTCTCG+GGG 0.491727 5.3:-79281226 None:intergenic
CAAGCAAACAACACTGAAGA+AGG 0.495908 5.3:+79281368 MS.gene38166:CDS
AATGTGGCAAAGCATGGAAA+AGG 0.503046 5.3:-79281484 None:intergenic
TCTTACAAGATTGTCAGCTA+TGG 0.505345 5.3:+79281848 MS.gene38166:CDS
ACGATTTGCTCCCAGCACTT+TGG 0.511706 5.3:-79281793 None:intergenic
GGCAACATTTGGTTTGTTGG+TGG 0.517913 5.3:+79281892 MS.gene38166:CDS
TCTGGTGTAGGTGCTGGTGC+TGG 0.520921 5.3:-79281248 None:intergenic
GCAACAGAGTGCAGCTCACA+AGG 0.522471 5.3:-79281157 None:intergenic
TCAGTGCCAAATATTGCCTC+AGG 0.530169 5.3:-79281704 None:intergenic
TCAGCTGGAGAATCAGCTGC+AGG 0.533442 5.3:-79281770 None:intergenic
GGTGCAGGAGCCGGAACAGG+TGG 0.536302 5.3:-79281731 None:intergenic
CTCCAGCTGACCAAAGTGCT+GGG 0.544197 5.3:+79281783 MS.gene38166:CDS
AATAGAAATGTGGCAAAGCA+TGG 0.549806 5.3:-79281490 None:intergenic
TTCAGGATGGTCAATCACTA+AGG 0.555642 5.3:+79281619 MS.gene38166:CDS
TAATCACCACCGGCAAATGT+TGG 0.556429 5.3:-79281554 None:intergenic
AGGTTAACAAAATCTGGTGT+AGG 0.563929 5.3:-79281260 None:intergenic
GCTTTGAGTTTGGATAAAGA+AGG 0.566467 5.3:-79281440 None:intergenic
ACAAAATCTGGTGTAGGTGC+TGG 0.568282 5.3:-79281254 None:intergenic
TGCAGGCGCAATATCTGGTG+TGG 0.569523 5.3:-79281753 None:intergenic
GAACTATCTGGTGAAGATGA+AGG 0.574002 5.3:-79281821 None:intergenic
AACCAAATGTTGCCAACACA+AGG 0.581175 5.3:-79281883 None:intergenic
GCAGGCGCAATATCTGGTGT+GGG 0.581910 5.3:-79281752 None:intergenic
ACCGGCAAATGTTGGAGTTG+AGG 0.585382 5.3:-79281546 None:intergenic
AGGTTTCTTCAATGATGCGA+AGG 0.588628 5.3:-79281420 None:intergenic
CTGTCAAAATTAATTCAGGA+TGG 0.599822 5.3:+79281606 MS.gene38166:CDS
GCCGGAGTTGGACTCTCGGG+GGG 0.600157 5.3:-79281224 None:intergenic
TCTGTTGCTTCTGAGCTCAT+CGG 0.605895 5.3:+79281172 MS.gene38166:CDS
ATTCAAAGTGTAATCACCAC+CGG 0.611570 5.3:-79281564 None:intergenic
AGCCGGAGTTGGACTCTCGG+GGG 0.617688 5.3:-79281225 None:intergenic
AATTTGGGGCAACCTTGTGT+TGG 0.628436 5.3:+79281871 MS.gene38166:CDS
ATTGTCAGCTATGGAATTTG+GGG 0.656452 5.3:+79281857 MS.gene38166:CDS
TCAGCTAGTGAATAGAAATG+TGG 0.662953 5.3:-79281500 None:intergenic
TCAACTCCAACATTTGCCGG+TGG 0.688338 5.3:+79281548 MS.gene38166:CDS
CAACAGTGAGTAATTCAGTG+AGG 0.749189 5.3:-79281280 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGAAGAAAAGTGTGAAA+TGG - chr5.3:79281311-79281330 None:intergenic 25.0%
!!! TGCAATTTATCAAGTTGATA+AGG + chr5.3:79281673-79281692 MS.gene38166:CDS 25.0%
CTGTCAAAATTAATTCAGGA+TGG + chr5.3:79281606-79281625 MS.gene38166:CDS 30.0%
GGAACTGTCAAAATTAATTC+AGG + chr5.3:79281602-79281621 MS.gene38166:CDS 30.0%
TCAACTTGATAAATTGCAAC+AGG - chr5.3:79281671-79281690 None:intergenic 30.0%
TTGAATTTCACCGATAATTC+TGG + chr5.3:79281581-79281600 MS.gene38166:CDS 30.0%
TTTGACAGTTCCAGAATTAT+CGG - chr5.3:79281594-79281613 None:intergenic 30.0%
AATAGAAATGTGGCAAAGCA+TGG - chr5.3:79281493-79281512 None:intergenic 35.0%
AGATTGTCAGCTATGGAATT+TGG + chr5.3:79281855-79281874 MS.gene38166:CDS 35.0%
AGGTTAACAAAATCTGGTGT+AGG - chr5.3:79281263-79281282 None:intergenic 35.0%
ATTCAAAGTGTAATCACCAC+CGG - chr5.3:79281567-79281586 None:intergenic 35.0%
ATTGTCAGCTATGGAATTTG+GGG + chr5.3:79281857-79281876 MS.gene38166:CDS 35.0%
GATTGTCAGCTATGGAATTT+GGG + chr5.3:79281856-79281875 MS.gene38166:CDS 35.0%
TCAGCTAGTGAATAGAAATG+TGG - chr5.3:79281503-79281522 None:intergenic 35.0%
TCAGTGAGGTTAACAAAATC+TGG - chr5.3:79281269-79281288 None:intergenic 35.0%
TCTTACAAGATTGTCAGCTA+TGG + chr5.3:79281848-79281867 MS.gene38166:CDS 35.0%
TTGTAAGAAGACGAACTATC+TGG - chr5.3:79281836-79281855 None:intergenic 35.0%
! CCTTCAATCCACAAAAGTTT+TGG + chr5.3:79281331-79281350 MS.gene38166:CDS 35.0%
! GCTTTGAGTTTGGATAAAGA+AGG - chr5.3:79281443-79281462 None:intergenic 35.0%
!! AATCTCATCAGCTTTGAGTT+TGG - chr5.3:79281453-79281472 None:intergenic 35.0%
!! CCAAAACTTTTGTGGATTGA+AGG - chr5.3:79281334-79281353 None:intergenic 35.0%
AACCAAATGTTGCCAACACA+AGG - chr5.3:79281886-79281905 None:intergenic 40.0%
AATGTGGCAAAGCATGGAAA+AGG - chr5.3:79281487-79281506 None:intergenic 40.0%
ACTGAATTACTCACTGTTGC+TGG + chr5.3:79281284-79281303 MS.gene38166:CDS 40.0%
AGGTTTCTTCAATGATGCGA+AGG - chr5.3:79281423-79281442 None:intergenic 40.0%
CAACAGTGAGTAATTCAGTG+AGG - chr5.3:79281283-79281302 None:intergenic 40.0%
CAAGCAAACAACACTGAAGA+AGG + chr5.3:79281368-79281387 MS.gene38166:CDS 40.0%
GAACTATCTGGTGAAGATGA+AGG - chr5.3:79281824-79281843 None:intergenic 40.0%
TTCAGGATGGTCAATCACTA+AGG + chr5.3:79281619-79281638 MS.gene38166:CDS 40.0%
TTGCTTCCTGAGGCAATATT+TGG + chr5.3:79281698-79281717 MS.gene38166:CDS 40.0%
! AACCTTGTGTTGGCAACATT+TGG + chr5.3:79281881-79281900 MS.gene38166:CDS 40.0%
! GAACGTGTCCAAAACTTTTG+TGG - chr5.3:79281342-79281361 None:intergenic 40.0%
! TGATAAGGTTTTGCTTCCTG+AGG + chr5.3:79281688-79281707 MS.gene38166:CDS 40.0%
!! CTTCTTCAGTGTTGTTTGCT+TGG - chr5.3:79281370-79281389 None:intergenic 40.0%
!! GTTGGCAACATTTGGTTTGT+TGG + chr5.3:79281889-79281908 MS.gene38166:CDS 40.0%
!!! CAGTGTTGTTTGCTTGGTTT+TGG - chr5.3:79281364-79281383 None:intergenic 40.0%
ACAAAATCTGGTGTAGGTGC+TGG - chr5.3:79281257-79281276 None:intergenic 45.0%
ATGTTGGAGTTGAGGCAGTT+TGG - chr5.3:79281541-79281560 None:intergenic 45.0%
CACTGATATTCCACCTGTTC+CGG + chr5.3:79281721-79281740 MS.gene38166:CDS 45.0%
TAATCACCACCGGCAAATGT+TGG - chr5.3:79281557-79281576 None:intergenic 45.0%
TCAGTGCCAAATATTGCCTC+AGG - chr5.3:79281707-79281726 None:intergenic 45.0%
! AATTTGGGGCAACCTTGTGT+TGG + chr5.3:79281871-79281890 MS.gene38166:CDS 45.0%
! GATGCGAAGGAACTGTCTTT+TGG - chr5.3:79281410-79281429 None:intergenic 45.0%
! TCTGTTGCTTCTGAGCTCAT+CGG + chr5.3:79281172-79281191 MS.gene38166:CDS 45.0%
!! GGCAACATTTGGTTTGTTGG+TGG + chr5.3:79281892-79281911 MS.gene38166:CDS 45.0%
ACCGGCAAATGTTGGAGTTG+AGG - chr5.3:79281549-79281568 None:intergenic 50.0%
GAGTTGAGGCAGTTTGGCTA+AGG - chr5.3:79281535-79281554 None:intergenic 50.0%
GCCTCAACTCCAACATTTGC+CGG + chr5.3:79281545-79281564 MS.gene38166:CDS 50.0%
TCAACTCCAACATTTGCCGG+TGG + chr5.3:79281548-79281567 MS.gene38166:CDS 50.0%
! ACGATTTGCTCCCAGCACTT+TGG - chr5.3:79281796-79281815 None:intergenic 50.0%
!! AGAGGGTGGACTTACGGTTT+TGG - chr5.3:79281204-79281223 None:intergenic 50.0%
GCAACAGAGTGCAGCTCACA+AGG - chr5.3:79281160-79281179 None:intergenic 55.0%
GCAATATCTGGTGTGGGTGC+AGG - chr5.3:79281749-79281768 None:intergenic 55.0%
GCAGGCGCAATATCTGGTGT+GGG - chr5.3:79281755-79281774 None:intergenic 55.0%
TCAGCTGCAGGCGCAATATC+TGG - chr5.3:79281761-79281780 None:intergenic 55.0%
TCAGCTGGAGAATCAGCTGC+AGG - chr5.3:79281773-79281792 None:intergenic 55.0%
TCTCCAGCTGACCAAAGTGC+TGG + chr5.3:79281782-79281801 MS.gene38166:CDS 55.0%
TGCAGGCGCAATATCTGGTG+TGG - chr5.3:79281756-79281775 None:intergenic 55.0%
! CTCCAGCTGACCAAAGTGCT+GGG + chr5.3:79281783-79281802 MS.gene38166:CDS 55.0%
CTCCCAGCACTTTGGTCAGC+TGG - chr5.3:79281788-79281807 None:intergenic 60.0%
TGGAGCCGGAGTTGGACTCT+CGG - chr5.3:79281231-79281250 None:intergenic 60.0%
!! TCTGGTGTAGGTGCTGGTGC+TGG - chr5.3:79281251-79281270 None:intergenic 60.0%
AGCCGGAGTTGGACTCTCGG+GGG - chr5.3:79281228-79281247 None:intergenic 65.0%
AGTTGGACTCTCGGGGGGAG+AGG - chr5.3:79281222-79281241 None:intergenic 65.0%
GAGCCGGAGTTGGACTCTCG+GGG - chr5.3:79281229-79281248 None:intergenic 65.0%
GGAGCCGGAGTTGGACTCTC+GGG - chr5.3:79281230-79281249 None:intergenic 65.0%
GTTGGACTCTCGGGGGGAGA+GGG - chr5.3:79281221-79281240 None:intergenic 65.0%
TCCCCCCGAGAGTCCAACTC+CGG + chr5.3:79281223-79281242 MS.gene38166:CDS 65.0%
TCTGGTGTGGGTGCAGGAGC+CGG - chr5.3:79281743-79281762 None:intergenic 65.0%
! GGGGGGAGAGGGTGGACTTA+CGG - chr5.3:79281210-79281229 None:intergenic 65.0%
!! GTAGGTGCTGGTGCTGGAGC+CGG - chr5.3:79281245-79281264 None:intergenic 65.0%
GCCGGAGTTGGACTCTCGGG+GGG - chr5.3:79281227-79281246 None:intergenic 70.0%
GGTGCAGGAGCCGGAACAGG+TGG - chr5.3:79281734-79281753 None:intergenic 70.0%
GTGGGTGCAGGAGCCGGAAC+AGG - chr5.3:79281737-79281756 None:intergenic 70.0%
! GCTGGTGCTGGAGCCGGAGT+TGG - chr5.3:79281239-79281258 None:intergenic 70.0%
GGACTCTCGGGGGGAGAGGG+TGG - chr5.3:79281218-79281237 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 79281137 79281925 79281137 ID=MS.gene38166
chr5.3 mRNA 79281137 79281925 79281137 ID=MS.gene38166.t1;Parent=MS.gene38166
chr5.3 exon 79281137 79281925 79281137 ID=MS.gene38166.t1.exon1;Parent=MS.gene38166.t1
chr5.3 CDS 79281137 79281925 79281137 ID=cds.MS.gene38166.t1;Parent=MS.gene38166.t1
Gene Sequence

>MS.gene38166

ATGAAGGTTTCATTGATTTTCCTTGTGAGCTGCACTCTGTTGCTTCTGAGCTCATCGGCATTTGCCAAAACCGTAAGTCCACCCTCTCCCCCCGAGAGTCCAACTCCGGCTCCAGCACCAGCACCTACACCAGATTTTGTTAACCTCACTGAATTACTCACTGTTGCTGGTCCATTTCACACTTTTCTTCAATACCTTCAATCCACAAAAGTTTTGGACACGTTCCAAAACCAAGCAAACAACACTGAAGAAGGAATCACTATCTTTGTTCCAAAAGACAGTTCCTTCGCATCATTGAAGAAACCTTCTTTATCCAAACTCAAAGCTGATGAGATTAAACAAGTGATCCTTTTCCATGCTTTGCCACATTTCTATTCACTAGCTGATTTCAAAAACCTTAGCCAAACTGCCTCAACTCCAACATTTGCCGGTGGTGATTACACTTTGAATTTCACCGATAATTCTGGAACTGTCAAAATTAATTCAGGATGGTCAATCACTAAGGTGACTAGTGCAGTTCATGCTACTGATCCTGTTGCAATTTATCAAGTTGATAAGGTTTTGCTTCCTGAGGCAATATTTGGCACTGATATTCCACCTGTTCCGGCTCCTGCACCCACACCAGATATTGCGCCTGCAGCTGATTCTCCAGCTGACCAAAGTGCTGGGAGCAAATCGTCTTCACCTTCATCTTCACCAGATAGTTCGTCTTCTTACAAGATTGTCAGCTATGGAATTTGGGGCAACCTTGTGTTGGCAACATTTGGTTTGTTGGTGGCACTCTTGTGA

Protein sequence

>MS.gene38166.t1

MKVSLIFLVSCTLLLLSSSAFAKTVSPPSPPESPTPAPAPAPTPDFVNLTELLTVAGPFHTFLQYLQSTKVLDTFQNQANNTEEGITIFVPKDSSFASLKKPSLSKLKADEIKQVILFHALPHFYSLADFKNLSQTASTPTFAGGDYTLNFTDNSGTVKINSGWSITKVTSAVHATDPVAIYQVDKVLLPEAIFGTDIPPVPAPAPTPDIAPAADSPADQSAGSKSSSPSSSPDSSSSYKIVSYGIWGNLVLATFGLLVALL