AlfalfaGEDB | MS.gene38554

M. sativa cultivar XinJiangDaYe / MS.gene38554

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38554.t1	XP_013461718.1	92.9	98	7	0	1	98	1	98	1.10E-36	162.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38554.t1	A0A072V157	92.9	98	7	0	1	98	1	98	7.7e-37	162.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050507	MS.gene38554	0.806243	9.04E-50	-1.69E-46
MS.gene050812	MS.gene38554	0.853616	2.12E-61	-1.69E-46
MS.gene05081	MS.gene38554	0.801431	9.03E-49	-1.69E-46
MS.gene05105	MS.gene38554	0.806852	6.73E-50	-1.69E-46
MS.gene051171	MS.gene38554	0.83607	1.16E-56	-1.69E-46
MS.gene051242	MS.gene38554	0.822462	2.34E-53	-1.69E-46
MS.gene051244	MS.gene38554	0.847512	1.10E-59	-1.69E-46
MS.gene051617	MS.gene38554	0.810812	9.57E-51	-1.69E-46
MS.gene051666	MS.gene38554	0.808221	3.44E-50	-1.69E-46
MS.gene052310	MS.gene38554	0.805005	1.64E-49	-1.69E-46
MS.gene052870	MS.gene38554	0.814723	1.33E-51	-1.69E-46
MS.gene052872	MS.gene38554	0.805725	1.16E-49	-1.69E-46
MS.gene053107	MS.gene38554	0.843011	1.82E-58	-1.69E-46
MS.gene053207	MS.gene38554	0.804503	2.09E-49	-1.69E-46
MS.gene053209	MS.gene38554	0.808056	3.74E-50	-1.69E-46
MS.gene055206	MS.gene38554	0.806206	9.21E-50	-1.69E-46
MS.gene055971	MS.gene38554	0.809532	1.81E-50	-1.69E-46
MS.gene056664	MS.gene38554	0.853358	2.52E-61	-1.69E-46
MS.gene056796	MS.gene38554	0.852891	3.43E-61	-1.69E-46
MS.gene056897	MS.gene38554	0.800124	1.67E-48	-1.69E-46
MS.gene056903	MS.gene38554	0.8352	1.93E-56	-1.69E-46
MS.gene057129	MS.gene38554	0.802416	5.67E-49	-1.69E-46
MS.gene057196	MS.gene38554	0.821899	3.16E-53	-1.69E-46
MS.gene057332	MS.gene38554	0.829363	5.37E-55	-1.69E-46
MS.gene057658	MS.gene38554	0.805171	1.52E-49	-1.69E-46
MS.gene058082	MS.gene38554	0.825813	3.82E-54	-1.69E-46
MS.gene058233	MS.gene38554	0.834833	2.39E-56	-1.69E-46
MS.gene058276	MS.gene38554	0.822408	2.41E-53	-1.69E-46
MS.gene058293	MS.gene38554	0.812746	3.63E-51	-1.69E-46
MS.gene058365	MS.gene38554	0.81421	1.73E-51	-1.69E-46
MS.gene058746	MS.gene38554	0.825278	5.11E-54	-1.69E-46
MS.gene058748	MS.gene38554	0.806984	6.31E-50	-1.69E-46
MS.gene059023	MS.gene38554	0.8143	1.65E-51	-1.69E-46
MS.gene05934	MS.gene38554	0.800487	1.41E-48	-1.69E-46
MS.gene059692	MS.gene38554	0.812378	4.37E-51	-1.69E-46
MS.gene059695	MS.gene38554	0.816018	6.86E-52	-1.69E-46
MS.gene059858	MS.gene38554	0.801629	8.23E-49	-1.69E-46
MS.gene059960	MS.gene38554	0.818772	1.65E-52	-1.69E-46
MS.gene060407	MS.gene38554	0.818484	1.91E-52	-1.69E-46
MS.gene06092	MS.gene38554	0.818016	2.44E-52	-1.69E-46
MS.gene061150	MS.gene38554	0.806667	7.36E-50	-1.69E-46
MS.gene061231	MS.gene38554	0.829502	4.97E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38554.t1	MTR_3g102270	95.181	83	4	16	98	16	98	5.12e-56	171
MS.gene38554.t1	MTR_3g102270	95.181	83	4	16	98	16	98	1.06e-55	171
MS.gene38554.t1	MTR_3g102270	95.181	83	4	16	98	16	98	9.71e-55	169

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 29 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGATTTAACGTGTTGTTAA+CGG	0.274705	3.1:+81114917	None:intergenic
AAGAATCTGATTATCGAAAA+TGG	0.276535	3.1:-81114854	MS.gene38554:CDS
TTGCGGCGCGCCATCGTTCA+TGG	0.324166	3.1:-81114995	MS.gene38554:CDS
TCCTTAGTAAGCACGATAAT+AGG	0.329251	3.1:+81114795	None:intergenic
CGGTGAAGTGTTTGTAGTTA+TGG	0.331589	3.1:+81115027	None:intergenic
TGGCCGCACCACTTCTTCTA+AGG	0.356825	3.1:-81115076	MS.gene38554:CDS
CCCGTCGAGTAACTTCCGTT+TGG	0.427801	3.1:-81114883	MS.gene38554:CDS
AACGTGTTGTTAACGGAGTT+AGG	0.441542	3.1:+81114924	None:intergenic
ACGGAGTTAGGTTTATCGTT+TGG	0.446191	3.1:+81114936	None:intergenic
GTCGAGTAACTTCCGTTTGG+AGG	0.460444	3.1:-81114880	MS.gene38554:CDS
GGATGGAGGGACATTAACTT+TGG	0.461982	3.1:-81114668	MS.gene38554:CDS
CCGTTAACAACACGTTAAAT+CGG	0.462228	3.1:-81114917	MS.gene38554:CDS
CAGATTCTTCATCCTCCAAA+CGG	0.535477	3.1:+81114868	None:intergenic
TGGAGGGACATTAACTTTGG+AGG	0.540847	3.1:-81114665	MS.gene38554:CDS
CGGCGCTGCACCATGAACGA+TGG	0.551636	3.1:+81114985	None:intergenic
AGAGCTTGCCTTAGAAGAAG+TGG	0.555098	3.1:+81115068	None:intergenic
CGACGGTGGATTGATGGCGG+CGG	0.566544	3.1:+81114965	None:intergenic
TGAACGATGGCGCGCCGCAA+CGG	0.567387	3.1:+81114998	None:intergenic
CGCCGCCATCAATCCACCGT+CGG	0.583751	3.1:-81114964	MS.gene38554:CDS
GCGCGCCGCAACGGTTCTGA+CGG	0.584424	3.1:+81115007	None:intergenic
TTCCGACGGTGGATTGATGG+CGG	0.592348	3.1:+81114962	None:intergenic
ATCTGATTATCGAAAATGGA+AGG	0.596386	3.1:-81114850	MS.gene38554:CDS
CCAAACGGAAGTTACTCGAC+GGG	0.599011	3.1:+81114883	None:intergenic
TTGTGTGCTAGGTATATGGA+TGG	0.625552	3.1:-81114685	MS.gene38554:intron
TCCAAACGGAAGTTACTCGA+CGG	0.646116	3.1:+81114882	None:intergenic
TGTGCTAGGTATATGGATGG+AGG	0.667189	3.1:-81114682	MS.gene38554:intron
GTGCTAGGTATATGGATGGA+GGG	0.681449	3.1:-81114681	MS.gene38554:intron
TTGCCTTAGAAGAAGTGGTG+CGG	0.691027	3.1:+81115073	None:intergenic
CTTCACCGTCAGAACCGTTG+CGG	0.698015	3.1:-81115012	MS.gene38554:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGAATCTGATTATCGAAAA+TGG	-	chr3.1:81114884-81114903	MS.gene38554:CDS	25.0%
!!!	TGAATTTAATTTTGTGTGCT+AGG	-	chr3.1:81115042-81115061	MS.gene38554:CDS	25.0%
	ATCTGATTATCGAAAATGGA+AGG	-	chr3.1:81114888-81114907	MS.gene38554:CDS	30.0%
!	AATTTTGTGTGCTAGGTATA+TGG	-	chr3.1:81115049-81115068	MS.gene38554:CDS	30.0%
	ACCTATTATCGTGCTTACTA+AGG	-	chr3.1:81114942-81114961	MS.gene38554:CDS	35.0%
	CCGTTAACAACACGTTAAAT+CGG	-	chr3.1:81114821-81114840	MS.gene38554:CDS	35.0%
	TCCTTAGTAAGCACGATAAT+AGG	+	chr3.1:81114946-81114965	None:intergenic	35.0%
!	CCGATTTAACGTGTTGTTAA+CGG	+	chr3.1:81114824-81114843	None:intergenic	35.0%
!!!	TTATCGTTTGGTTTTTCCGA+CGG	+	chr3.1:81114793-81114812	None:intergenic	35.0%
	ACGGAGTTAGGTTTATCGTT+TGG	+	chr3.1:81114805-81114824	None:intergenic	40.0%
	CAGATTCTTCATCCTCCAAA+CGG	+	chr3.1:81114873-81114892	None:intergenic	40.0%
	TTGTGTGCTAGGTATATGGA+TGG	-	chr3.1:81115053-81115072	MS.gene38554:CDS	40.0%
!	AACGTGTTGTTAACGGAGTT+AGG	+	chr3.1:81114817-81114836	None:intergenic	40.0%
!	CGGTGAAGTGTTTGTAGTTA+TGG	+	chr3.1:81114714-81114733	None:intergenic	40.0%
	AGAGCTTGCCTTAGAAGAAG+TGG	+	chr3.1:81114673-81114692	None:intergenic	45.0%
	GGATGGAGGGACATTAACTT+TGG	-	chr3.1:81115070-81115089	MS.gene38554:CDS	45.0%
	GTGCTAGGTATATGGATGGA+GGG	-	chr3.1:81115057-81115076	MS.gene38554:CDS	45.0%
	TCCAAACGGAAGTTACTCGA+CGG	+	chr3.1:81114859-81114878	None:intergenic	45.0%
	TGGAGGGACATTAACTTTGG+AGG	-	chr3.1:81115073-81115092	MS.gene38554:CDS	45.0%
	TGTGCTAGGTATATGGATGG+AGG	-	chr3.1:81115056-81115075	MS.gene38554:CDS	45.0%
	TTGCCTTAGAAGAAGTGGTG+CGG	+	chr3.1:81114668-81114687	None:intergenic	45.0%
!!!	TTTTTCCGACGGTGGATTGA+TGG	+	chr3.1:81114782-81114801	None:intergenic	45.0%
	CCAAACGGAAGTTACTCGAC+GGG	+	chr3.1:81114858-81114877	None:intergenic	50.0%
	GTCGAGTAACTTCCGTTTGG+AGG	-	chr3.1:81114858-81114877	MS.gene38554:CDS	50.0%
!!!	TCGTTTGGTTTTTCCGACGG+TGG	+	chr3.1:81114790-81114809	None:intergenic	50.0%
	CCCGTCGAGTAACTTCCGTT+TGG	-	chr3.1:81114855-81114874	MS.gene38554:CDS	55.0%
	CTTCACCGTCAGAACCGTTG+CGG	-	chr3.1:81114726-81114745	MS.gene38554:intron	55.0%
	TGGCCGCACCACTTCTTCTA+AGG	-	chr3.1:81114662-81114681	MS.gene38554:CDS	55.0%
!!	TTCCGACGGTGGATTGATGG+CGG	+	chr3.1:81114779-81114798	None:intergenic	55.0%
	CGCCGCCATCAATCCACCGT+CGG	-	chr3.1:81114774-81114793	MS.gene38554:intron	65.0%
	CGGCGCTGCACCATGAACGA+TGG	+	chr3.1:81114756-81114775	None:intergenic	65.0%
	TGAACGATGGCGCGCCGCAA+CGG	+	chr3.1:81114743-81114762	None:intergenic	65.0%
	TTGCGGCGCGCCATCGTTCA+TGG	-	chr3.1:81114743-81114762	MS.gene38554:intron	65.0%
!!	CGACGGTGGATTGATGGCGG+CGG	+	chr3.1:81114776-81114795	None:intergenic	65.0%
!	GCGCGCCGCAACGGTTCTGA+CGG	+	chr3.1:81114734-81114753	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	81114661	81115099	81114661	ID=MS.gene38554
chr3.1	mRNA	81114661	81115099	81114661	ID=MS.gene38554.t1;Parent=MS.gene38554
chr3.1	exon	81114809	81115099	81114809	ID=MS.gene38554.t1.exon1;Parent=MS.gene38554.t1
chr3.1	CDS	81114809	81115099	81114809	ID=cds.MS.gene38554.t1;Parent=MS.gene38554.t1
chr3.1	exon	81114661	81114696	81114661	ID=MS.gene38554.t1.exon2;Parent=MS.gene38554.t1
chr3.1	CDS	81114661	81114696	81114661	ID=cds.MS.gene38554.t1;Parent=MS.gene38554.t1

Gene Sequence

>MS.gene38554

ATGGCCGCACCACTTCTTCTAAGGCAAGCTCTGTTAATTCGCGCTTGTTTCCATAACTACAAACACTTCACCGTCAGAACCGTTGCGGCGCGCCATCGTTCATGGTGCAGCGCCGCCGCCATCAATCCACCGTCGGAAAAACCAAACGATAAACCTAACTCCGTTAACAACACGTTAAATCGGAGAGATGTTTACCCGTCGAGTAACTTCCGTTTGGAGGATGAAGAATCTGATTATCGAAAATGGAAGGATAAAGAAGATGAGATTTTCAATGATATTGAACCTATTATCGTGCTTACTAAGGAAATTCTTCATTCATCAAAGTAAAATTCGTGTTCTGTCTTCATTTCAATGAATTGCGATTTTTTTTTATTGATTTATTGAATTTAATTTTGTGTGCTAGGTATATGGATGGAGGGACATTAACTTTGGAGGATGA

Protein sequence

>MS.gene38554.t1

MAAPLLLRQALLIRACFHNYKHFTVRTVAARHRSWCSAAAINPPSEKPNDKPNSVNNTLNRRDVYPSSNFRLEDEESDYRKWKDKEDEIFNDIEPIIVYGWRDINFGG