Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38760.t1 | XP_003625000.1 | 93.1 | 131 | 6 | 1 | 38 | 168 | 38 | 165 | 8.20E-60 | 240 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38760.t1 | O80669 | 36.6 | 131 | 55 | 4 | 39 | 168 | 34 | 137 | 3.3e-09 | 63.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38760.t1 | G7KQS8 | 93.1 | 131 | 6 | 1 | 38 | 168 | 38 | 165 | 5.9e-60 | 240.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene38760 | 0.800542 | 1.37E-48 | -1.69E-46 |
| MS.gene049993 | MS.gene38760 | 0.838434 | 2.88E-57 | -1.69E-46 |
| MS.gene050397 | MS.gene38760 | 0.807373 | 5.22E-50 | -1.69E-46 |
| MS.gene050406 | MS.gene38760 | 0.821535 | 3.83E-53 | -1.69E-46 |
| MS.gene050464 | MS.gene38760 | 0.803772 | 2.97E-49 | -1.69E-46 |
| MS.gene051582 | MS.gene38760 | 0.80185 | 7.41E-49 | -1.69E-46 |
| MS.gene052011 | MS.gene38760 | -0.808611 | 2.84E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene38760 | 0.820774 | 5.74E-53 | -1.69E-46 |
| MS.gene052253 | MS.gene38760 | 0.831682 | 1.45E-55 | -1.69E-46 |
| MS.gene053836 | MS.gene38760 | 0.828218 | 1.02E-54 | -1.69E-46 |
| MS.gene054011 | MS.gene38760 | 0.808475 | 3.04E-50 | -1.69E-46 |
| MS.gene054102 | MS.gene38760 | 0.805852 | 1.09E-49 | -1.69E-46 |
| MS.gene05412 | MS.gene38760 | 0.814358 | 1.60E-51 | -1.69E-46 |
| MS.gene05413 | MS.gene38760 | 0.843421 | 1.42E-58 | -1.69E-46 |
| MS.gene054183 | MS.gene38760 | 0.843387 | 1.45E-58 | -1.69E-46 |
| MS.gene054918 | MS.gene38760 | 0.827934 | 1.19E-54 | -1.69E-46 |
| MS.gene055277 | MS.gene38760 | 0.80558 | 1.25E-49 | -1.69E-46 |
| MS.gene055428 | MS.gene38760 | 0.808868 | 2.51E-50 | -1.69E-46 |
| MS.gene05553 | MS.gene38760 | 0.825734 | 3.99E-54 | -1.69E-46 |
| MS.gene056612 | MS.gene38760 | 0.81566 | 8.25E-52 | -1.69E-46 |
| MS.gene056899 | MS.gene38760 | -0.810075 | 1.38E-50 | -1.69E-46 |
| MS.gene05702 | MS.gene38760 | 0.876099 | 1.83E-68 | -1.69E-46 |
| MS.gene057396 | MS.gene38760 | 0.81186 | 5.67E-51 | -1.69E-46 |
| MS.gene057825 | MS.gene38760 | 0.805281 | 1.44E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene38760 | 0.801501 | 8.74E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene38760 | 0.814286 | 1.66E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene38760 | 0.819552 | 1.09E-52 | -1.69E-46 |
| MS.gene059420 | MS.gene38760 | 0.82134 | 4.25E-53 | -1.69E-46 |
| MS.gene059894 | MS.gene38760 | 0.813454 | 2.54E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene38760 | 0.802327 | 5.91E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene38760 | 0.873443 | 1.46E-67 | -1.69E-46 |
| MS.gene061159 | MS.gene38760 | 0.802989 | 4.32E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38760.t1 | MTR_7g089860 | 92.913 | 127 | 6 | 1 | 42 | 168 | 42 | 165 | 1.43e-81 | 238 |
| MS.gene38760.t1 | MTR_8g040080 | 57.627 | 59 | 20 | 2 | 40 | 95 | 12 | 68 | 7.54e-12 | 59.7 |
| MS.gene38760.t1 | MTR_3g013980 | 83.871 | 31 | 5 | 0 | 42 | 72 | 22 | 52 | 6.54e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38760.t1 | AT2G41180 | 35.897 | 156 | 67 | 6 | 10 | 161 | 4 | 130 | 1.71e-18 | 77.4 |
| MS.gene38760.t1 | AT3G56710 | 60.000 | 55 | 21 | 1 | 18 | 72 | 17 | 70 | 9.59e-14 | 65.5 |
| MS.gene38760.t1 | AT3G56710 | 60.000 | 55 | 21 | 1 | 18 | 72 | 17 | 70 | 9.59e-14 | 65.5 |
Find 48 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGACTAGTGCCTCTGAATTT+AGG | 0.151197 | 7.4:-18916952 | MS.gene38760:CDS |
| GCCAAACCCTTCCAGGTTTC+AGG | 0.192379 | 7.4:-18916888 | MS.gene38760:CDS |
| CGTCCTGACCGGTGAGTTCT+TGG | 0.262694 | 7.4:+18916921 | None:intergenic |
| GACTAGTGCCTCTGAATTTA+GGG | 0.269705 | 7.4:-18916951 | MS.gene38760:CDS |
| GCACTAGTCTTAACCTTCAT+AGG | 0.284756 | 7.4:+18916965 | None:intergenic |
| GATATGTACACTACCTTGAT+AGG | 0.301968 | 7.4:+18916992 | None:intergenic |
| AATCGCCGCCAAACCCTTCC+AGG | 0.314881 | 7.4:-18916895 | MS.gene38760:CDS |
| TTGGGTTTGGTCAGCTTTGT+TGG | 0.315333 | 7.4:+18917049 | None:intergenic |
| CTGAGATGATGGATAACATT+TGG | 0.318960 | 7.4:-18916679 | MS.gene38760:CDS |
| CTAGGTTTCCCTTTATCATC+AGG | 0.381649 | 7.4:+18916761 | None:intergenic |
| TCCTGAAACCTGGAAGGGTT+TGG | 0.394232 | 7.4:+18916887 | None:intergenic |
| TACCTTGATAGGTTTGTTGT+AGG | 0.408553 | 7.4:+18917003 | None:intergenic |
| TGAGATGATGGATAACATTT+GGG | 0.436367 | 7.4:-18916678 | MS.gene38760:CDS |
| AGTACTAATCACTGATGAAC+TGG | 0.446531 | 7.4:+18917087 | None:intergenic |
| TTGTGGATCCTGATGATAAA+GGG | 0.446940 | 7.4:-18916769 | MS.gene38760:CDS |
| CTTGTGGATCCTGATGATAA+AGG | 0.466204 | 7.4:-18916770 | MS.gene38760:CDS |
| TGCCTCTGAATTTAGGGCAC+TGG | 0.466630 | 7.4:-18916945 | MS.gene38760:CDS |
| AAAAGTGATCAAACAGTTGT+GGG | 0.481052 | 7.4:-18916803 | MS.gene38760:CDS |
| CACTAGTCTTAACCTTCATA+GGG | 0.488788 | 7.4:+18916966 | None:intergenic |
| CGATGATAGCGGTACTGACC+GGG | 0.505073 | 7.4:-18916855 | MS.gene38760:CDS |
| CACCTACAACAAACCTATCA+AGG | 0.508296 | 7.4:-18917005 | MS.gene38760:CDS |
| AAAGGGAAACCTAGTGAAGC+TGG | 0.510780 | 7.4:-18916752 | MS.gene38760:CDS |
| GATTCATAGAATGCACTTGT+GGG | 0.511701 | 7.4:+18916647 | None:intergenic |
| GGTCCAAGAACTCACCGGTC+AGG | 0.513511 | 7.4:-18916924 | MS.gene38760:CDS |
| GGCGTCAAACTCCTGAAACC+TGG | 0.514749 | 7.4:+18916877 | None:intergenic |
| CAAATGTTATCCATCATCTC+AGG | 0.515560 | 7.4:+18916680 | None:intergenic |
| TAAATCACTAGCTATCAAGT+TGG | 0.523443 | 7.4:+18916621 | None:intergenic |
| CCGATGATAGCGGTACTGAC+CGG | 0.529604 | 7.4:-18916856 | MS.gene38760:CDS |
| CGGCGATTCAGCGTCCTGAC+CGG | 0.538040 | 7.4:+18916910 | None:intergenic |
| TTTGATGAGCCAGCTTCACT+AGG | 0.548731 | 7.4:+18916743 | None:intergenic |
| GTACATATCAAACCCTATGA+AGG | 0.558200 | 7.4:-18916978 | MS.gene38760:CDS |
| TAAAAGTGATCAAACAGTTG+TGG | 0.559766 | 7.4:-18916804 | MS.gene38760:CDS |
| TGAAACCTGGAAGGGTTTGG+CGG | 0.569700 | 7.4:+18916890 | None:intergenic |
| CCGGTCAGTACCGCTATCAT+CGG | 0.574024 | 7.4:+18916856 | None:intergenic |
| TCATCAGGATCCACAAGAGA+AGG | 0.581877 | 7.4:+18916776 | None:intergenic |
| TCAAACTCCTGAAACCTGGA+AGG | 0.582081 | 7.4:+18916881 | None:intergenic |
| AGATTCATAGAATGCACTTG+TGG | 0.611006 | 7.4:+18916646 | None:intergenic |
| GAGTTTGACGCCGATGATAG+CGG | 0.617254 | 7.4:-18916866 | MS.gene38760:CDS |
| GGGTGGATGTGAGAATAGGA+TGG | 0.620443 | 7.4:-18916834 | MS.gene38760:CDS |
| ACCGGGGTGGATGTGAGAAT+AGG | 0.636045 | 7.4:-18916838 | MS.gene38760:CDS |
| AAGTGATCAAACAGTTGTGG+GGG | 0.637078 | 7.4:-18916801 | MS.gene38760:CDS |
| CAAACTCCTGAAACCTGGAA+GGG | 0.640399 | 7.4:+18916882 | None:intergenic |
| GACCAGTGCCCTAAATTCAG+AGG | 0.644343 | 7.4:+18916943 | None:intergenic |
| TCCTATTCTCACATCCACCC+CGG | 0.646942 | 7.4:+18916837 | None:intergenic |
| AAAGTGATCAAACAGTTGTG+GGG | 0.657329 | 7.4:-18916802 | MS.gene38760:CDS |
| GATGATAGCGGTACTGACCG+GGG | 0.684269 | 7.4:-18916854 | MS.gene38760:CDS |
| GCACTGGTCCAAGAACTCAC+CGG | 0.712892 | 7.4:-18916929 | MS.gene38760:CDS |
| GATAGCGGTACTGACCGGGG+TGG | 0.738191 | 7.4:-18916851 | MS.gene38760:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCATCAAATGAAAGTTTTGA+GGG | - | chr7.4:18917012-18917031 | MS.gene38760:CDS | 25.0% |
| !!! | TTGTTGTTGTTTTTCTTCTT+GGG | + | chr7.4:18916712-18916731 | None:intergenic | 25.0% |
| AAAAGTGATCAAACAGTTGT+GGG | - | chr7.4:18916937-18916956 | MS.gene38760:CDS | 30.0% | |
| TAAAAGTGATCAAACAGTTG+TGG | - | chr7.4:18916936-18916955 | MS.gene38760:CDS | 30.0% | |
| ! | CTCATCAAATGAAAGTTTTG+AGG | - | chr7.4:18917011-18917030 | MS.gene38760:CDS | 30.0% |
| !! | TGAGATGATGGATAACATTT+GGG | - | chr7.4:18917062-18917081 | MS.gene38760:CDS | 30.0% |
| !!! | GTTGTTGTTGTTTTTCTTCT+TGG | + | chr7.4:18916713-18916732 | None:intergenic | 30.0% |
| AAAGTGATCAAACAGTTGTG+GGG | - | chr7.4:18916938-18916957 | MS.gene38760:CDS | 35.0% | |
| AGATTCATAGAATGCACTTG+TGG | + | chr7.4:18917097-18917116 | None:intergenic | 35.0% | |
| AGTACTAATCACTGATGAAC+TGG | + | chr7.4:18916656-18916675 | None:intergenic | 35.0% | |
| CAAATGTTATCCATCATCTC+AGG | + | chr7.4:18917063-18917082 | None:intergenic | 35.0% | |
| GATATGTACACTACCTTGAT+AGG | + | chr7.4:18916751-18916770 | None:intergenic | 35.0% | |
| GATTCATAGAATGCACTTGT+GGG | + | chr7.4:18917096-18917115 | None:intergenic | 35.0% | |
| GTACATATCAAACCCTATGA+AGG | - | chr7.4:18916762-18916781 | MS.gene38760:CDS | 35.0% | |
| TTGTGGATCCTGATGATAAA+GGG | - | chr7.4:18916971-18916990 | MS.gene38760:CDS | 35.0% | |
| !! | CACTAGTCTTAACCTTCATA+GGG | + | chr7.4:18916777-18916796 | None:intergenic | 35.0% |
| !! | CTGAGATGATGGATAACATT+TGG | - | chr7.4:18917061-18917080 | MS.gene38760:CDS | 35.0% |
| !! | TACCTTGATAGGTTTGTTGT+AGG | + | chr7.4:18916740-18916759 | None:intergenic | 35.0% |
| !!! | GTTGTTTTTCTTCTTGGGTT+TGG | + | chr7.4:18916707-18916726 | None:intergenic | 35.0% |
| AAGTGATCAAACAGTTGTGG+GGG | - | chr7.4:18916939-18916958 | MS.gene38760:CDS | 40.0% | |
| AGACTAGTGCCTCTGAATTT+AGG | - | chr7.4:18916788-18916807 | MS.gene38760:CDS | 40.0% | |
| CACCTACAACAAACCTATCA+AGG | - | chr7.4:18916735-18916754 | MS.gene38760:CDS | 40.0% | |
| CTAGGTTTCCCTTTATCATC+AGG | + | chr7.4:18916982-18917001 | None:intergenic | 40.0% | |
| CTTGTGGATCCTGATGATAA+AGG | - | chr7.4:18916970-18916989 | MS.gene38760:CDS | 40.0% | |
| GACTAGTGCCTCTGAATTTA+GGG | - | chr7.4:18916789-18916808 | MS.gene38760:CDS | 40.0% | |
| !! | GCACTAGTCTTAACCTTCAT+AGG | + | chr7.4:18916778-18916797 | None:intergenic | 40.0% |
| AAAGGGAAACCTAGTGAAGC+TGG | - | chr7.4:18916988-18917007 | MS.gene38760:CDS | 45.0% | |
| CAAACTCCTGAAACCTGGAA+GGG | + | chr7.4:18916861-18916880 | None:intergenic | 45.0% | |
| TCAAACTCCTGAAACCTGGA+AGG | + | chr7.4:18916862-18916881 | None:intergenic | 45.0% | |
| TCATCAGGATCCACAAGAGA+AGG | + | chr7.4:18916967-18916986 | None:intergenic | 45.0% | |
| TTTGATGAGCCAGCTTCACT+AGG | + | chr7.4:18917000-18917019 | None:intergenic | 45.0% | |
| ! | ACTTTTGACGCCTGAGATGA+TGG | - | chr7.4:18917050-18917069 | MS.gene38760:CDS | 45.0% |
| ! | TTGGGTTTGGTCAGCTTTGT+TGG | + | chr7.4:18916694-18916713 | None:intergenic | 45.0% |
| GACCAGTGCCCTAAATTCAG+AGG | + | chr7.4:18916800-18916819 | None:intergenic | 50.0% | |
| GAGTTTGACGCCGATGATAG+CGG | - | chr7.4:18916874-18916893 | MS.gene38760:CDS | 50.0% | |
| GGGTGGATGTGAGAATAGGA+TGG | - | chr7.4:18916906-18916925 | MS.gene38760:CDS | 50.0% | |
| TCCTATTCTCACATCCACCC+CGG | + | chr7.4:18916906-18916925 | None:intergenic | 50.0% | |
| TCCTGAAACCTGGAAGGGTT+TGG | + | chr7.4:18916856-18916875 | None:intergenic | 50.0% | |
| TGAAACCTGGAAGGGTTTGG+CGG | + | chr7.4:18916853-18916872 | None:intergenic | 50.0% | |
| ! | TGCCTCTGAATTTAGGGCAC+TGG | - | chr7.4:18916795-18916814 | MS.gene38760:CDS | 50.0% |
| ACCGGGGTGGATGTGAGAAT+AGG | - | chr7.4:18916902-18916921 | MS.gene38760:CDS | 55.0% | |
| CCGGTCAGTACCGCTATCAT+CGG | + | chr7.4:18916887-18916906 | None:intergenic | 55.0% | |
| GCCAAACCCTTCCAGGTTTC+AGG | - | chr7.4:18916852-18916871 | MS.gene38760:CDS | 55.0% | |
| GGCGTCAAACTCCTGAAACC+TGG | + | chr7.4:18916866-18916885 | None:intergenic | 55.0% | |
| ! | GCACTGGTCCAAGAACTCAC+CGG | - | chr7.4:18916811-18916830 | MS.gene38760:CDS | 55.0% |
| !! | CCGATGATAGCGGTACTGAC+CGG | - | chr7.4:18916884-18916903 | MS.gene38760:CDS | 55.0% |
| !! | CGATGATAGCGGTACTGACC+GGG | - | chr7.4:18916885-18916904 | MS.gene38760:CDS | 55.0% |
| !! | GATGATAGCGGTACTGACCG+GGG | - | chr7.4:18916886-18916905 | MS.gene38760:CDS | 55.0% |
| AATCGCCGCCAAACCCTTCC+AGG | - | chr7.4:18916845-18916864 | MS.gene38760:CDS | 60.0% | |
| CGTCCTGACCGGTGAGTTCT+TGG | + | chr7.4:18916822-18916841 | None:intergenic | 60.0% | |
| GGTCCAAGAACTCACCGGTC+AGG | - | chr7.4:18916816-18916835 | MS.gene38760:CDS | 60.0% | |
| !! | TGTGGGGGTGCCTTCTCTTG+TGG | - | chr7.4:18916954-18916973 | MS.gene38760:CDS | 60.0% |
| CGGCGATTCAGCGTCCTGAC+CGG | + | chr7.4:18916833-18916852 | None:intergenic | 65.0% | |
| !! | GATAGCGGTACTGACCGGGG+TGG | - | chr7.4:18916889-18916908 | MS.gene38760:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 18916628 | 18917134 | 18916628 | ID=MS.gene38760 |
| chr7.4 | mRNA | 18916628 | 18917134 | 18916628 | ID=MS.gene38760.t1;Parent=MS.gene38760 |
| chr7.4 | exon | 18916628 | 18917134 | 18916628 | ID=MS.gene38760.t1.exon1;Parent=MS.gene38760.t1 |
| chr7.4 | CDS | 18916628 | 18917134 | 18916628 | ID=cds.MS.gene38760.t1;Parent=MS.gene38760.t1 |
| Gene Sequence |
| Protein sequence |
