Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38850.t1 | RHN48916.1 | 90.7 | 97 | 8 | 1 | 47 | 142 | 224 | 320 | 1.70E-37 | 165.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38850.t1 | Q94AH6 | 77.3 | 97 | 21 | 1 | 47 | 142 | 573 | 669 | 1.9e-34 | 146.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38850.t1 | G7KRT0 | 90.7 | 97 | 8 | 1 | 47 | 142 | 570 | 666 | 1.2e-37 | 165.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene38850 | 0.833374 | 5.53E-56 | -1.69E-46 |
| MS.gene049587 | MS.gene38850 | 0.811518 | 6.73E-51 | -1.69E-46 |
| MS.gene049853 | MS.gene38850 | 0.873764 | 1.14E-67 | -1.69E-46 |
| MS.gene051575 | MS.gene38850 | 0.801425 | 9.06E-49 | -1.69E-46 |
| MS.gene052447 | MS.gene38850 | 0.822441 | 2.37E-53 | -1.69E-46 |
| MS.gene05329 | MS.gene38850 | 0.812409 | 4.30E-51 | -1.69E-46 |
| MS.gene05404 | MS.gene38850 | 0.800043 | 1.73E-48 | -1.69E-46 |
| MS.gene054174 | MS.gene38850 | 0.853807 | 1.87E-61 | -1.69E-46 |
| MS.gene054175 | MS.gene38850 | 0.857949 | 1.15E-62 | -1.69E-46 |
| MS.gene054484 | MS.gene38850 | 0.842522 | 2.46E-58 | -1.69E-46 |
| MS.gene055017 | MS.gene38850 | 0.820025 | 8.53E-53 | -1.69E-46 |
| MS.gene055056 | MS.gene38850 | 0.837524 | 4.94E-57 | -1.69E-46 |
| MS.gene055058 | MS.gene38850 | 0.831635 | 1.49E-55 | -1.69E-46 |
| MS.gene05531 | MS.gene38850 | 0.812867 | 3.42E-51 | -1.69E-46 |
| MS.gene055952 | MS.gene38850 | 0.814694 | 1.35E-51 | -1.69E-46 |
| MS.gene058515 | MS.gene38850 | 0.849797 | 2.57E-60 | -1.69E-46 |
| MS.gene058580 | MS.gene38850 | 0.818265 | 2.15E-52 | -1.69E-46 |
| MS.gene059276 | MS.gene38850 | 0.853455 | 2.36E-61 | -1.69E-46 |
| MS.gene059458 | MS.gene38850 | 0.813107 | 3.03E-51 | -1.69E-46 |
| MS.gene059460 | MS.gene38850 | 0.800269 | 1.56E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38850.t1 | MTR_7g108340 | 90.722 | 97 | 8 | 1 | 47 | 142 | 570 | 666 | 4.17e-52 | 176 |
| MS.gene38850.t1 | MTR_4g119413 | 77.660 | 94 | 20 | 1 | 50 | 142 | 582 | 675 | 1.66e-43 | 152 |
| MS.gene38850.t1 | MTR_2g437390 | 76.596 | 94 | 21 | 1 | 50 | 142 | 580 | 673 | 1.71e-43 | 152 |
| MS.gene38850.t1 | MTR_2g019260 | 30.833 | 120 | 67 | 2 | 16 | 124 | 588 | 702 | 4.57e-12 | 62.8 |
| MS.gene38850.t1 | MTR_2g019260 | 30.833 | 120 | 67 | 2 | 16 | 124 | 588 | 702 | 4.83e-12 | 62.4 |
| MS.gene38850.t1 | MTR_5g040040 | 38.144 | 97 | 56 | 2 | 32 | 124 | 411 | 507 | 3.30e-11 | 60.1 |
| MS.gene38850.t1 | MTR_2g018360 | 66.667 | 39 | 13 | 0 | 88 | 126 | 30 | 68 | 3.90e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38850.t1 | AT4G02570 | 77.320 | 97 | 21 | 1 | 47 | 142 | 573 | 669 | 4.79e-45 | 156 |
| MS.gene38850.t1 | AT4G02570 | 77.320 | 97 | 21 | 1 | 47 | 142 | 573 | 669 | 4.79e-45 | 156 |
| MS.gene38850.t1 | AT4G02570 | 77.320 | 97 | 21 | 1 | 47 | 142 | 573 | 669 | 4.79e-45 | 156 |
| MS.gene38850.t1 | AT4G02570 | 77.320 | 97 | 21 | 1 | 47 | 142 | 573 | 669 | 4.79e-45 | 156 |
| MS.gene38850.t1 | AT1G43140 | 70.103 | 97 | 28 | 1 | 47 | 142 | 556 | 652 | 1.21e-39 | 141 |
| MS.gene38850.t1 | AT1G02980 | 68.085 | 94 | 29 | 1 | 50 | 142 | 580 | 673 | 6.83e-38 | 136 |
| MS.gene38850.t1 | AT1G02980 | 68.085 | 94 | 29 | 1 | 50 | 142 | 580 | 673 | 6.83e-38 | 136 |
| MS.gene38850.t1 | AT5G46210 | 31.667 | 120 | 66 | 2 | 16 | 124 | 588 | 702 | 2.16e-13 | 66.6 |
Find 27 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAACTCAAAGCTGTTATT+TGG | 0.120691 | 8.4:-52093028 | None:intergenic |
| CTTCCGCTCAATCGCGCTTT+TGG | 0.258241 | 8.4:+52092624 | MS.gene38850:CDS |
| GGGGATATTGTTCTAGTGTT+AGG | 0.298977 | 8.4:-52093007 | None:intergenic |
| TGAACTCAAAGCTGTTATTT+GGG | 0.303552 | 8.4:-52093027 | None:intergenic |
| TGATAAAATGAGAAGGATAA+AGG | 0.328384 | 8.4:+52093063 | MS.gene38850:CDS |
| TCATTTGCAGAGCAGCAAAT+TGG | 0.384248 | 8.4:+52092543 | MS.gene38850:CDS |
| ATTTGAAGATTCTCTTCTAC+AGG | 0.429642 | 8.4:+52092844 | MS.gene38850:intron |
| ATAACCTTCCTTTCATCGAC+AGG | 0.464796 | 8.4:-52093199 | None:intergenic |
| AAAATATATGATGCTTGATA+AGG | 0.466748 | 8.4:+52092982 | MS.gene38850:CDS |
| TTGACCGTTGAACCGATTCA+AGG | 0.487199 | 8.4:-52092583 | None:intergenic |
| CCGCAGTGCTGAATAGTAGC+AGG | 0.487589 | 8.4:-52092874 | None:intergenic |
| CTTTCATCGACAGGCGGAAG+AGG | 0.488133 | 8.4:-52093190 | None:intergenic |
| CAAAAGCGCGATTGAGCGGA+AGG | 0.515414 | 8.4:-52092623 | None:intergenic |
| GAGAATGAAGCAATCTAACT+AGG | 0.520531 | 8.4:-52092949 | None:intergenic |
| CTCTTCCGCCTGTCGATGAA+AGG | 0.522925 | 8.4:+52093191 | MS.gene38850:CDS |
| AGCGCGATTGAGCGGAAGGG+TGG | 0.540009 | 8.4:-52092619 | None:intergenic |
| TCACCAAAAGCGCGATTGAG+CGG | 0.556194 | 8.4:-52092627 | None:intergenic |
| GAACTCAAAGCTGTTATTTG+GGG | 0.565800 | 8.4:-52093026 | None:intergenic |
| TTTCATCGACAGGCGGAAGA+GGG | 0.573315 | 8.4:-52093189 | None:intergenic |
| ATCACCTTGAATCGGTTCAA+CGG | 0.575542 | 8.4:+52092579 | MS.gene38850:CDS |
| GCGCGATTGAGCGGAAGGGT+GGG | 0.585850 | 8.4:-52092618 | None:intergenic |
| TCCGCCTGTCGATGAAAGGA+AGG | 0.598612 | 8.4:+52093195 | MS.gene38850:CDS |
| CGCAGTGCTGAATAGTAGCA+GGG | 0.616738 | 8.4:-52092873 | None:intergenic |
| ACCTTCCTTTCATCGACAGG+CGG | 0.628723 | 8.4:-52093196 | None:intergenic |
| AAAAGCGCGATTGAGCGGAA+GGG | 0.653250 | 8.4:-52092622 | None:intergenic |
| AATTTACTGATAAAATGAGA+AGG | 0.656477 | 8.4:+52093056 | MS.gene38850:CDS |
| CCTGCTACTATTCAGCACTG+CGG | 0.795381 | 8.4:+52092874 | MS.gene38850:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAACAAAATACAACTAA+TGG | + | chr8.4:52092694-52092713 | MS.gene38850:intron | 15.0% |
| !! | AAAATATATGATGCTTGATA+AGG | + | chr8.4:52092982-52093001 | MS.gene38850:CDS | 20.0% |
| !! | AATTTACTGATAAAATGAGA+AGG | + | chr8.4:52093056-52093075 | MS.gene38850:CDS | 20.0% |
| !!! | AGTTTCAGATTTTTATTAAC+AGG | - | chr8.4:52093154-52093173 | None:intergenic | 20.0% |
| !!! | GTATTTTGTTAATTAATGAC+CGG | - | chr8.4:52092688-52092707 | None:intergenic | 20.0% |
| !!! | GTTTCAGATTTTTATTAACA+GGG | - | chr8.4:52093153-52093172 | None:intergenic | 20.0% |
| !!! | TCTCATTTTATCAGTAAATT+TGG | - | chr8.4:52093056-52093075 | None:intergenic | 20.0% |
| !!! | TTTGGTGAATGTATTTTTAA+AGG | + | chr8.4:52092642-52092661 | MS.gene38850:CDS | 20.0% |
| ! | AAAAAAAGATCACCTTGAAT+CGG | + | chr8.4:52092571-52092590 | MS.gene38850:CDS | 25.0% |
| ! | CTAAGTTCAAAATCTTATGA+GGG | - | chr8.4:52093106-52093125 | None:intergenic | 25.0% |
| ! | TGATAAAATGAGAAGGATAA+AGG | + | chr8.4:52093063-52093082 | MS.gene38850:CDS | 25.0% |
| ATTTGAAGATTCTCTTCTAC+AGG | + | chr8.4:52092844-52092863 | MS.gene38850:intron | 30.0% | |
| GCTAAGTTCAAAATCTTATG+AGG | - | chr8.4:52093107-52093126 | None:intergenic | 30.0% | |
| TGAGTAAATAGAAAACCATG+AGG | - | chr8.4:52092732-52092751 | None:intergenic | 30.0% | |
| TGTTATCATCATCTTCACTT+GGG | + | chr8.4:52092754-52092773 | MS.gene38850:intron | 30.0% | |
| TTGAACTCAAAGCTGTTATT+TGG | - | chr8.4:52093031-52093050 | None:intergenic | 30.0% | |
| TTGTTATCATCATCTTCACT+TGG | + | chr8.4:52092753-52092772 | MS.gene38850:intron | 30.0% | |
| ! | TGAACTCAAAGCTGTTATTT+GGG | - | chr8.4:52093030-52093049 | None:intergenic | 30.0% |
| GAGAATGAAGCAATCTAACT+AGG | - | chr8.4:52092952-52092971 | None:intergenic | 35.0% | |
| ! | GAACTCAAAGCTGTTATTTG+GGG | - | chr8.4:52093029-52093048 | None:intergenic | 35.0% |
| AAGAGAAGAGGATGAGTCTT+AGG | - | chr8.4:52092794-52092813 | None:intergenic | 40.0% | |
| ATAACCTTCCTTTCATCGAC+AGG | - | chr8.4:52093202-52093221 | None:intergenic | 40.0% | |
| ATACGCTCTTGATTCTCTTC+CGG | + | chr8.4:52092666-52092685 | MS.gene38850:intron | 40.0% | |
| GGGAAAAAGAATTGAGAACC+TGG | - | chr8.4:52093133-52093152 | None:intergenic | 40.0% | |
| GGGGATATTGTTCTAGTGTT+AGG | - | chr8.4:52093010-52093029 | None:intergenic | 40.0% | |
| TCAGCATAACAGAAGAGAAG+AGG | - | chr8.4:52092806-52092825 | None:intergenic | 40.0% | |
| TCATTTGCAGAGCAGCAAAT+TGG | + | chr8.4:52092543-52092562 | MS.gene38850:CDS | 40.0% | |
| TGGTTATGTTGTGTTCCTCA+TGG | + | chr8.4:52092714-52092733 | MS.gene38850:intron | 40.0% | |
| ! | ATCACCTTGAATCGGTTCAA+CGG | + | chr8.4:52092579-52092598 | MS.gene38850:CDS | 40.0% |
| ! | GATTTTGAACTTAGCTAGCC+AGG | + | chr8.4:52093112-52093131 | MS.gene38850:intron | 40.0% |
| GAAGAGGATGAGTCTTAGGA+AGG | - | chr8.4:52092790-52092809 | None:intergenic | 45.0% | |
| ! | TTGACCGTTGAACCGATTCA+AGG | - | chr8.4:52092586-52092605 | None:intergenic | 45.0% |
| ACCTTCCTTTCATCGACAGG+CGG | - | chr8.4:52093199-52093218 | None:intergenic | 50.0% | |
| CCTGCTACTATTCAGCACTG+CGG | + | chr8.4:52092874-52092893 | MS.gene38850:CDS | 50.0% | |
| TTTCATCGACAGGCGGAAGA+GGG | - | chr8.4:52093192-52093211 | None:intergenic | 50.0% | |
| ! | AAAAGCGCGATTGAGCGGAA+GGG | - | chr8.4:52092625-52092644 | None:intergenic | 50.0% |
| ! | CGCAGTGCTGAATAGTAGCA+GGG | - | chr8.4:52092876-52092895 | None:intergenic | 50.0% |
| ! | TCACCAAAAGCGCGATTGAG+CGG | - | chr8.4:52092630-52092649 | None:intergenic | 50.0% |
| ACAGGCGGAAGAGGGATCTA+TGG | - | chr8.4:52093184-52093203 | None:intergenic | 55.0% | |
| CTCTTCCGCCTGTCGATGAA+AGG | + | chr8.4:52093191-52093210 | MS.gene38850:CDS | 55.0% | |
| CTTTCATCGACAGGCGGAAG+AGG | - | chr8.4:52093193-52093212 | None:intergenic | 55.0% | |
| TCCGCCTGTCGATGAAAGGA+AGG | + | chr8.4:52093195-52093214 | MS.gene38850:CDS | 55.0% | |
| ! | CAAAAGCGCGATTGAGCGGA+AGG | - | chr8.4:52092626-52092645 | None:intergenic | 55.0% |
| ! | CCGCAGTGCTGAATAGTAGC+AGG | - | chr8.4:52092877-52092896 | None:intergenic | 55.0% |
| ! | CTTCCGCTCAATCGCGCTTT+TGG | + | chr8.4:52092624-52092643 | MS.gene38850:CDS | 55.0% |
| AGCGCGATTGAGCGGAAGGG+TGG | - | chr8.4:52092622-52092641 | None:intergenic | 65.0% | |
| GCGCGATTGAGCGGAAGGGT+GGG | - | chr8.4:52092621-52092640 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 52092528 | 52093246 | 52092528 | ID=MS.gene38850 |
| chr8.4 | mRNA | 52092528 | 52093246 | 52092528 | ID=MS.gene38850.t1;Parent=MS.gene38850 |
| chr8.4 | exon | 52092528 | 52092663 | 52092528 | ID=MS.gene38850.t1.exon1;Parent=MS.gene38850.t1 |
| chr8.4 | CDS | 52092528 | 52092663 | 52092528 | ID=cds.MS.gene38850.t1;Parent=MS.gene38850.t1 |
| chr8.4 | exon | 52092852 | 52093084 | 52092852 | ID=MS.gene38850.t1.exon2;Parent=MS.gene38850.t1 |
| chr8.4 | CDS | 52092852 | 52093084 | 52092852 | ID=cds.MS.gene38850.t1;Parent=MS.gene38850.t1 |
| chr8.4 | exon | 52093187 | 52093246 | 52093187 | ID=MS.gene38850.t1.exon3;Parent=MS.gene38850.t1 |
| chr8.4 | CDS | 52093187 | 52093246 | 52093187 | ID=cds.MS.gene38850.t1;Parent=MS.gene38850.t1 |
| Gene Sequence |
| Protein sequence |
