AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39680.t1 MTR_8g009170 91.935 124 10 0 1 124 1 124 1.13e-78 234
MS.gene39680.t1 MTR_8g009160 90.323 124 12 0 1 124 1 124 1.19e-78 236
MS.gene39680.t1 MTR_8g009170 91.935 124 10 0 1 124 1 124 4.54e-78 234
MS.gene39680.t1 MTR_8g009110 89.516 124 13 0 1 124 1 124 1.74e-77 233
MS.gene39680.t1 MTR_8g008930 90.323 124 12 0 1 124 1 124 4.42e-77 232
MS.gene39680.t1 MTR_8g009090 89.516 124 13 0 1 124 1 124 2.17e-76 230
MS.gene39680.t1 MTR_8g028435 87.903 124 15 0 1 124 1 124 9.20e-76 229
MS.gene39680.t1 MTR_8g009120 84.677 124 19 0 1 124 1 124 1.20e-71 218
MS.gene39680.t1 MTR_8g009150 83.871 124 20 0 1 124 1 124 1.30e-71 217
MS.gene39680.t1 MTR_4g117880 75.806 124 30 0 1 124 1 124 2.00e-65 202
MS.gene39680.t1 MTR_2g069090 70.796 113 29 2 15 124 20 131 3.28e-49 160
MS.gene39680.t1 MTR_2g069210 64.286 126 40 3 2 124 4 127 1.04e-46 154
MS.gene39680.t1 MTR_2g069190 64.602 113 36 2 15 124 20 131 1.07e-44 149
MS.gene39680.t1 MTR_2g069300 62.832 113 38 2 15 124 6 117 2.88e-43 145
MS.gene39680.t1 MTR_1g043770 59.813 107 41 2 19 124 11 116 2.55e-39 133
MS.gene39680.t1 MTR_1g043770 59.813 107 41 2 19 124 11 116 4.57e-39 134
MS.gene39680.t1 MTR_1g043760 59.091 110 42 2 17 124 9 117 8.04e-38 131
MS.gene39680.t1 MTR_1g044105 53.913 115 45 2 17 124 9 122 3.81e-37 124
MS.gene39680.t1 MTR_1g043775 58.182 110 43 2 17 124 9 117 6.04e-37 127
MS.gene39680.t1 MTR_1g043775 58.182 110 43 2 17 124 9 117 1.44e-36 127
MS.gene39680.t1 MTR_8g009080 58.197 122 46 4 6 124 38 157 4.90e-36 125
MS.gene39680.t1 MTR_8g009080 58.197 122 46 4 6 124 38 157 8.18e-36 125
MS.gene39680.t1 MTR_8g009080 58.197 122 46 4 6 124 38 157 1.84e-35 125
MS.gene39680.t1 MTR_1g043810 53.636 110 48 2 17 124 9 117 1.92e-34 121
MS.gene39680.t1 MTR_1g043810 53.636 110 48 2 17 124 9 117 3.44e-34 121
MS.gene39680.t1 MTR_1g093140 45.255 137 56 5 4 124 3 136 3.23e-32 116
MS.gene39680.t1 MTR_7g078010 50.000 110 52 2 17 124 22 130 2.06e-31 113
MS.gene39680.t1 MTR_7g078010 50.000 110 52 2 17 124 22 130 9.75e-31 112
MS.gene39680.t1 MTR_4g024660 48.305 118 55 3 12 124 14 130 1.24e-30 112
MS.gene39680.t1 MTR_1g093120 46.087 115 53 4 17 124 18 130 2.47e-30 111
MS.gene39680.t1 MTR_8g063070 47.059 119 57 4 10 123 12 129 2.63e-30 111
MS.gene39680.t1 MTR_8g006930 49.091 110 53 2 17 124 22 130 5.29e-30 110
MS.gene39680.t1 MTR_8g062600 47.200 125 50 4 10 124 12 130 5.57e-30 110
MS.gene39680.t1 MTR_8g041600 53.509 114 45 3 17 124 22 133 7.86e-30 109
MS.gene39680.t1 MTR_7g056347 48.214 112 55 3 15 124 20 130 8.11e-30 110
MS.gene39680.t1 MTR_4g119410 47.458 118 56 4 12 124 14 130 2.18e-29 109
MS.gene39680.t1 MTR_8g041600 53.509 114 45 3 17 124 22 133 3.28e-29 108
MS.gene39680.t1 MTR_4g099380 48.649 111 45 3 19 124 129 232 4.09e-29 106
MS.gene39680.t1 MTR_8g041620 53.509 114 45 3 17 124 22 133 5.86e-29 108
MS.gene39680.t1 MTR_2g068950 43.750 128 66 3 1 124 1 126 1.30e-28 107
MS.gene39680.t1 MTR_2g068960 46.094 128 63 4 1 124 1 126 1.13e-27 104
MS.gene39680.t1 MTR_1g093110 45.299 117 53 4 17 124 19 133 1.94e-27 103
MS.gene39680.t1 MTR_7g029655 43.966 116 54 2 15 123 15 126 1.95e-25 98.6
MS.gene39680.t1 MTR_2g069010 40.708 113 62 2 16 124 21 132 2.34e-24 95.9
MS.gene39680.t1 MTR_2g069020 42.478 113 60 2 16 124 19 130 3.01e-24 95.5
MS.gene39680.t1 MTR_2g069020 42.478 113 60 2 16 124 19 130 4.40e-24 95.1
MS.gene39680.t1 MTR_7g056393 44.545 110 58 2 17 124 22 130 4.92e-24 94.7
MS.gene39680.t1 MTR_1g070185 41.667 108 63 0 17 124 23 130 6.30e-24 94.4
MS.gene39680.t1 MTR_8g469400 44.000 125 52 4 5 124 7 118 4.34e-23 92.0
MS.gene39680.t1 MTR_0032s0040 45.378 119 51 2 15 123 15 129 8.16e-23 91.7
MS.gene39680.t1 MTR_7g056370 38.739 111 61 3 18 124 2 109 4.08e-22 87.0
MS.gene39680.t1 MTR_1g032140 37.600 125 70 2 4 124 6 126 3.66e-20 84.3
MS.gene39680.t1 MTR_7g105070 39.815 108 60 2 19 123 15 120 4.15e-20 84.0
MS.gene39680.t1 MTR_1g032250 38.182 110 60 2 19 124 28 133 4.52e-20 84.0
MS.gene39680.t1 MTR_1g032220 38.182 110 60 2 19 124 28 133 2.03e-19 82.4
MS.gene39680.t1 MTR_1g043840 61.111 54 21 0 71 124 256 309 2.57e-18 79.0
MS.gene39680.t1 MTR_7g068650 35.398 113 66 3 19 124 6 118 3.46e-18 79.0
MS.gene39680.t1 MTR_2g069070 40.541 111 45 3 16 124 21 112 9.00e-18 75.1
MS.gene39680.t1 MTR_2g068850 39.394 99 54 3 27 123 21 115 3.49e-16 71.6
MS.gene39680.t1 MTR_2g069060 40.541 111 45 3 16 124 21 112 3.50e-16 73.2
MS.gene39680.t1 MTR_2g068840 39.394 99 54 3 27 123 21 115 3.94e-15 70.5
MS.gene39680.t1 MTR_2g068800 39.560 91 51 2 27 116 21 108 8.99e-15 68.9
MS.gene39680.t1 MTR_3g091350 33.858 127 70 3 3 124 6 123 4.11e-14 67.4
MS.gene39680.t1 MTR_2g069060 45.070 71 38 1 54 124 21 90 5.21e-13 63.5
MS.gene39680.t1 MTR_4g015790 28.689 122 71 2 19 124 5 126 9.55e-13 63.5
MS.gene39680.t1 MTR_3g070860 28.440 109 75 1 19 124 6 114 3.23e-12 62.0
MS.gene39680.t1 MTR_1g034140 46.154 65 29 1 60 124 27 85 3.62e-12 62.0
MS.gene39680.t1 MTR_4g087130 41.176 68 37 1 60 124 26 93 7.31e-12 61.2
MS.gene39680.t1 MTR_4g087120 42.647 68 36 1 60 124 26 93 1.71e-11 60.1
MS.gene39680.t1 MTR_3g091360 32.075 106 69 2 22 124 11 116 3.90e-11 58.9
MS.gene39680.t1 MTR_4g087140 42.647 68 36 1 60 124 26 93 4.30e-11 58.9
MS.gene39680.t1 MTR_7g056353 33.333 105 42 3 21 124 28 105 6.80e-11 58.5
MS.gene39680.t1 MTR_5g048740 36.275 102 55 5 27 124 10 105 8.05e-11 58.2
MS.gene39680.t1 MTR_5g055800 34.000 100 60 3 27 124 12 107 8.50e-11 58.2
MS.gene39680.t1 MTR_5g055690 34.653 101 58 4 27 124 12 107 8.56e-11 58.2
MS.gene39680.t1 MTR_5g055690 34.653 101 58 4 27 124 12 107 8.83e-11 57.8
MS.gene39680.t1 MTR_5g082750 36.923 65 41 0 60 124 49 113 8.94e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39680.t1 AT5G59540 53.509 114 48 2 15 124 12 124 6.63e-35 122
MS.gene39680.t1 AT5G59540 53.509 114 48 2 15 124 12 124 2.16e-34 122
MS.gene39680.t1 AT5G59530 52.174 115 47 4 15 124 11 122 3.96e-31 113
MS.gene39680.t1 AT1G06640 48.855 131 54 5 4 124 1 128 4.97e-31 112
MS.gene39680.t1 AT1G06620 51.376 109 49 2 19 124 18 125 5.60e-31 111
MS.gene39680.t1 AT1G06640 48.855 131 54 5 4 124 1 128 5.82e-31 112
MS.gene39680.t1 AT1G06645 48.361 122 56 2 9 124 21 141 6.39e-31 113
MS.gene39680.t1 AT1G06640 48.855 131 54 5 4 124 1 128 1.64e-30 112
MS.gene39680.t1 AT1G06620 51.376 109 49 2 19 124 18 125 1.83e-30 112
MS.gene39680.t1 AT1G03410 48.246 114 53 2 16 124 45 157 2.28e-30 112
MS.gene39680.t1 AT1G03410 48.246 114 53 2 16 124 8 120 6.87e-30 110
MS.gene39680.t1 AT5G43440 48.276 116 52 2 15 124 11 124 1.58e-29 108
MS.gene39680.t1 AT1G04350 47.826 115 52 2 14 124 8 118 2.03e-29 109
MS.gene39680.t1 AT5G43440 48.276 116 52 2 15 124 11 124 5.51e-29 108
MS.gene39680.t1 AT1G06650 47.826 115 54 2 16 124 14 128 1.22e-28 106
MS.gene39680.t1 AT2G30830 47.706 109 56 1 17 124 10 118 2.04e-28 106
MS.gene39680.t1 AT1G06650 47.826 115 54 2 16 124 14 128 2.20e-28 106
MS.gene39680.t1 AT3G61400 41.406 128 68 2 4 124 1 128 5.14e-28 104
MS.gene39680.t1 AT3G61400 41.406 128 68 2 4 124 1 128 1.10e-27 104
MS.gene39680.t1 AT2G30840 46.491 114 56 2 16 124 9 122 1.72e-27 103
MS.gene39680.t1 AT2G30840 46.491 114 56 2 16 124 9 122 3.63e-27 103
MS.gene39680.t1 AT2G30840 46.491 114 56 2 16 124 9 122 3.85e-27 103
MS.gene39680.t1 AT2G25450 46.847 111 56 2 16 124 9 118 1.35e-26 101
MS.gene39680.t1 AT5G43450 45.217 115 53 3 15 123 11 121 2.00e-26 100
MS.gene39680.t1 AT5G43450 45.217 115 53 3 15 123 11 121 5.18e-26 100
MS.gene39680.t1 AT1G03400 43.363 113 51 3 16 124 8 111 3.48e-23 92.4
MS.gene39680.t1 AT1G03400 43.363 113 51 3 16 124 8 111 4.08e-23 92.4
MS.gene39680.t1 AT1G04380 40.909 110 47 3 16 124 13 105 1.47e-20 84.3
MS.gene39680.t1 AT1G04380 40.909 110 47 3 16 124 13 105 2.95e-20 84.3
MS.gene39680.t1 AT1G55290 39.048 105 48 5 27 124 25 120 1.07e-13 66.2
MS.gene39680.t1 AT3G13610 36.538 104 51 4 27 124 26 120 1.08e-13 66.2
MS.gene39680.t1 AT2G38240 30.275 109 67 2 20 124 4 107 1.17e-12 63.5
MS.gene39680.t1 AT3G12900 36.082 97 61 1 27 123 21 116 2.03e-12 62.8
MS.gene39680.t1 AT3G19010 44.928 69 36 1 55 123 23 89 2.15e-12 62.8
MS.gene39680.t1 AT3G19010 44.928 69 36 1 55 123 23 89 2.15e-12 62.8
MS.gene39680.t1 AT3G19010 44.928 69 36 1 55 123 23 89 2.19e-12 62.4
MS.gene39680.t1 AT3G19010 44.928 69 36 1 55 123 23 89 2.93e-12 62.0

Find 29 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTATTATCAATATCTTTA+AGG 0.122806 8.3:+79935907 None:intergenic
CCTCATATTTGTCTGGTTGA+TGG 0.257369 8.3:+79935967 None:intergenic
TTGGCTTCCTCATATTTGTC+TGG 0.377614 8.3:+79935960 None:intergenic
GTTAGGTTTGGTTGTATCAA+TGG 0.379115 8.3:+79936091 None:intergenic
ACAATTCCTTGATGAATACT+TGG 0.419418 8.3:+79935882 None:intergenic
TCAAGGAAGCATGTGAAACT+TGG 0.431792 8.3:-79935851 MS.gene39680:CDS
TTGAATGTGGAATCAAGGTT+AGG 0.444552 8.3:+79936074 None:intergenic
GTTCTTGAGGAGGTGAAAGA+TGG 0.464397 8.3:-79935789 MS.gene39680:CDS
GTTCATTGAATGTGGAATCA+AGG 0.469424 8.3:+79936069 None:intergenic
AATAACATGACTTGTGTTGT+TGG 0.488088 8.3:+79935941 None:intergenic
TGTGGAATCAAGGTTAGGTT+TGG 0.492547 8.3:+79936079 None:intergenic
CCCTTTGAGTGTTCTTGAGG+AGG 0.493576 8.3:-79935799 MS.gene39680:CDS
CAAGGAAGCATGTGAAACTT+GGG 0.494320 8.3:-79935850 MS.gene39680:CDS
AGGAATTGTTGACGATATCA+AGG 0.530296 8.3:-79935868 MS.gene39680:CDS
GAAACAAAAGCAGGTGTGAA+AGG 0.532657 8.3:-79936032 MS.gene39680:CDS
CATATTTGTCTGGTTGATGG+TGG 0.543038 8.3:+79935970 None:intergenic
TGCTTTGCGTTCATTGAATG+TGG 0.544374 8.3:+79936061 None:intergenic
AATCCCTTTGAGTGTTCTTG+AGG 0.545874 8.3:-79935802 MS.gene39680:CDS
ACCTCCTCAAGAACACTCAA+AGG 0.547379 8.3:+79935798 None:intergenic
CCTCCTCAAGAACACTCAAA+GGG 0.551009 8.3:+79935799 None:intergenic
CCATCAACCAGACAAATATG+AGG 0.555026 8.3:-79935967 MS.gene39680:CDS
AGGTGAAAGATGGAGTGAAG+AGG 0.558342 8.3:-79935779 MS.gene39680:CDS
CAATTCCTTGATGAATACTT+GGG 0.576394 8.3:+79935883 None:intergenic
ATATCTTTAAGGTCGATGAC+AGG 0.590653 8.3:+79935918 None:intergenic
GTGAAAGGACTTGTTGATGC+AGG 0.590786 8.3:-79936017 MS.gene39680:CDS
GCATTTGATGAAACAAAAGC+AGG 0.595830 8.3:-79936041 MS.gene39680:CDS
AAAGACCCAAGTATTCATCA+AGG 0.600194 8.3:-79935888 MS.gene39680:CDS
GGTTGATGGTGGAATAAGCT+TGG 0.607514 8.3:+79935981 None:intergenic
AAGGAAGCATGTGAAACTTG+GGG 0.628246 8.3:-79935849 MS.gene39680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTTATTATCAATATCTTTA+AGG + chr8.3:79935942-79935961 None:intergenic 15.0%
!!! TTTTTTCAAGTTGTTAATCA+TGG - chr8.3:79936021-79936040 MS.gene39680:CDS 20.0%
ACAATTCCTTGATGAATACT+TGG + chr8.3:79935967-79935986 None:intergenic 30.0%
CAATTCCTTGATGAATACTT+GGG + chr8.3:79935966-79935985 None:intergenic 30.0%
! AATAACATGACTTGTGTTGT+TGG + chr8.3:79935908-79935927 None:intergenic 30.0%
AAAGACCCAAGTATTCATCA+AGG - chr8.3:79935958-79935977 MS.gene39680:CDS 35.0%
AGGAATTGTTGACGATATCA+AGG - chr8.3:79935978-79935997 MS.gene39680:CDS 35.0%
GCATTTGATGAAACAAAAGC+AGG - chr8.3:79935805-79935824 MS.gene39680:CDS 35.0%
GTTCATTGAATGTGGAATCA+AGG + chr8.3:79935780-79935799 None:intergenic 35.0%
TTGAATGTGGAATCAAGGTT+AGG + chr8.3:79935775-79935794 None:intergenic 35.0%
! GTTAGGTTTGGTTGTATCAA+TGG + chr8.3:79935758-79935777 None:intergenic 35.0%
!! ATATCTTTAAGGTCGATGAC+AGG + chr8.3:79935931-79935950 None:intergenic 35.0%
AATCCCTTTGAGTGTTCTTG+AGG - chr8.3:79936044-79936063 MS.gene39680:CDS 40.0%
CCATCAACCAGACAAATATG+AGG - chr8.3:79935879-79935898 MS.gene39680:CDS 40.0%
CCTCATATTTGTCTGGTTGA+TGG + chr8.3:79935882-79935901 None:intergenic 40.0%
GAAACAAAAGCAGGTGTGAA+AGG - chr8.3:79935814-79935833 MS.gene39680:CDS 40.0%
TGCTTTGCGTTCATTGAATG+TGG + chr8.3:79935788-79935807 None:intergenic 40.0%
TGTGGAATCAAGGTTAGGTT+TGG + chr8.3:79935770-79935789 None:intergenic 40.0%
TTGGCTTCCTCATATTTGTC+TGG + chr8.3:79935889-79935908 None:intergenic 40.0%
! AAGGAAGCATGTGAAACTTG+GGG - chr8.3:79935997-79936016 MS.gene39680:CDS 40.0%
! CAAGGAAGCATGTGAAACTT+GGG - chr8.3:79935996-79936015 MS.gene39680:CDS 40.0%
! TCAAGGAAGCATGTGAAACT+TGG - chr8.3:79935995-79936014 MS.gene39680:CDS 40.0%
!! CATATTTGTCTGGTTGATGG+TGG + chr8.3:79935879-79935898 None:intergenic 40.0%
ACCTCCTCAAGAACACTCAA+AGG + chr8.3:79936051-79936070 None:intergenic 45.0%
AGGTGAAAGATGGAGTGAAG+AGG - chr8.3:79936067-79936086 MS.gene39680:CDS 45.0%
CCTCCTCAAGAACACTCAAA+GGG + chr8.3:79936050-79936069 None:intergenic 45.0%
GTTCTTGAGGAGGTGAAAGA+TGG - chr8.3:79936057-79936076 MS.gene39680:CDS 45.0%
!! GGTTGATGGTGGAATAAGCT+TGG + chr8.3:79935868-79935887 None:intergenic 45.0%
!! GTGAAAGGACTTGTTGATGC+AGG - chr8.3:79935829-79935848 MS.gene39680:CDS 45.0%
CCCTTTGAGTGTTCTTGAGG+AGG - chr8.3:79936047-79936066 MS.gene39680:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 79935748 79936120 79935748 ID=MS.gene39680
chr8.3 mRNA 79935748 79936120 79935748 ID=MS.gene39680.t1;Parent=MS.gene39680
chr8.3 exon 79935748 79936120 79935748 ID=MS.gene39680.t1.exon1;Parent=MS.gene39680.t1
chr8.3 CDS 79935748 79936120 79935748 ID=cds.MS.gene39680.t1;Parent=MS.gene39680.t1
Gene Sequence

>MS.gene39680

ATGGGAACCATTGATACAACCAAACCTAACCTTGATTCCACATTCAATGAACGCAAAGCATTTGATGAAACAAAAGCAGGTGTGAAAGGACTTGTTGATGCAGGAGTTGAGAAGATACCAAGCTTATTCCACCATCAACCAGACAAATATGAGGAAGCCAACAACACAAGTCATGTTATTCCTGTCATCGACCTTAAAGATATTGATAATAAAGACCCAAGTATTCATCAAGGAATTGTTGACGATATCAAGGAAGCATGTGAAACTTGGGGTTTTTTTCAAGTTGTTAATCATGGAATCCCTTTGAGTGTTCTTGAGGAGGTGAAAGATGGAGTGAAGAGGTTTTATGAACAAGATACAGAAGTCAAAAAAA

Protein sequence

>MS.gene39680.t1

MGTIDTTKPNLDSTFNERKAFDETKAGVKGLVDAGVEKIPSLFHHQPDKYEEANNTSHVIPVIDLKDIDNKDPSIHQGIVDDIKEACETWGFFQVVNHGIPLSVLEEVKDGVKRFYEQDTEVKK