AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40493


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40493.t1 MTR_5g006950 93.827 81 5 0 1 81 17 97 6.70e-47 150
MS.gene40493.t1 MTR_1g103490 79.012 81 17 0 1 81 17 97 3.19e-38 127
MS.gene40493.t1 MTR_7g095230 69.231 78 22 1 6 81 25 102 5.39e-28 102
MS.gene40493.t1 MTR_1g103450 59.756 82 30 2 1 81 17 96 2.83e-24 92.0
MS.gene40493.t1 MTR_1g103470 61.538 78 28 1 4 81 20 95 1.51e-23 90.5
MS.gene40493.t1 MTR_1g097720 72.881 59 13 2 1 58 17 73 2.04e-19 77.8
MS.gene40493.t1 MTR_1g071730 63.750 80 29 0 2 81 18 97 2.42e-16 70.5
MS.gene40493.t1 MTR_1g071730 63.750 80 29 0 2 81 18 97 5.78e-16 70.5
MS.gene40493.t1 MTR_5g070360 40.278 72 40 2 12 81 35 105 3.17e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40493.t1 AT3G22600 66.667 75 25 0 7 81 23 97 1.64e-28 103
MS.gene40493.t1 AT4G14815 61.842 76 29 0 6 81 20 95 1.31e-25 95.5
MS.gene40493.t1 AT2G48130 52.632 76 36 0 6 81 24 99 1.40e-19 80.9
MS.gene40493.t1 AT2G48130 52.632 76 36 0 6 81 24 99 1.40e-19 80.9
MS.gene40493.t1 AT2G48130 52.632 76 36 0 6 81 24 99 1.40e-19 80.9
MS.gene40493.t1 AT2G48130 52.632 76 36 0 6 81 24 99 1.40e-19 80.9
MS.gene40493.t1 AT2G48130 52.632 76 36 0 6 81 24 99 1.40e-19 80.9
MS.gene40493.t1 AT2G48130 52.632 76 36 0 6 81 24 99 1.40e-19 80.9
MS.gene40493.t1 AT1G03103 46.988 83 42 2 1 81 13 95 3.12e-14 66.6
MS.gene40493.t1 AT5G09370 39.474 76 42 2 8 81 24 97 4.97e-13 62.4
MS.gene40493.t1 AT5G09370 39.474 76 42 2 8 81 24 97 8.49e-13 62.4
MS.gene40493.t1 AT5G09370 39.474 76 42 2 8 81 24 97 8.91e-13 62.4
MS.gene40493.t1 AT3G43720 39.286 84 46 2 4 82 30 113 6.06e-12 60.8
MS.gene40493.t1 AT3G43720 39.286 84 46 2 4 82 30 113 7.67e-12 60.8

Find 50 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCCGTTGGTTCTTATTT+TGG 0.199878 5.3:+1920956 MS.gene40493:CDS
GTTCCTGCTGGTGAGTCTGC+TGG 0.326138 5.3:-1920733 None:intergenic
GTACCTGATGGAACTGAGTT+TGG 0.355549 5.3:-1920769 None:intergenic
AATTCCTCAACCCCGTCTTC+AGG 0.363035 5.3:+1920293 MS.gene40493:CDS
TTCCTGCTGGTGAGTCTGCT+GGG 0.376602 5.3:-1920732 None:intergenic
TACTGATTCTTTAATCAGTT+GGG 0.384390 5.3:+1921008 MS.gene40493:CDS
CAAAAGAATAGCATTTCAAA+TGG 0.424229 5.3:-1921034 None:intergenic
GACTCTGCTTCTGTTCCTGC+TGG 0.439322 5.3:-1920745 None:intergenic
GCCTATGTCAGGTTCTCAGC+GGG 0.446999 5.3:+1920360 MS.gene40493:CDS
AGCCTTGCCTGGTGCTTGCA+AGG 0.452642 5.3:+1920427 MS.gene40493:CDS
TCCATCACCGGTTGAGGGAG+TGG 0.468250 5.3:-1920904 None:intergenic
TCAAAATCCACTCCCTCAAC+CGG 0.469317 5.3:+1920897 MS.gene40493:CDS
ATACTGATTCTTTAATCAGT+TGG 0.471273 5.3:+1921007 MS.gene40493:CDS
GGATGATCCATCACCGGTTG+AGG 0.472460 5.3:-1920910 None:intergenic
CAGCTTGATTGAATTTCCAC+TGG 0.474292 5.3:-1920931 None:intergenic
GTTTGCACCTTGCAAGCACC+AGG 0.476162 5.3:-1920434 None:intergenic
TTGAGCAGCATCCTGAAGAC+GGG 0.489604 5.3:-1920304 None:intergenic
TCCACTCCCTCAACCGGTGA+TGG 0.490607 5.3:+1920903 MS.gene40493:CDS
TATAGTTAAGACAAGGTGAT+AGG 0.493717 5.3:-1920262 None:intergenic
ACTCAGGCTCTAGCCTTGCC+TGG 0.500358 5.3:+1920416 MS.gene40493:CDS
CACCTTGCAAGCACCAGGCA+AGG 0.514952 5.3:-1920429 None:intergenic
ATCGAGTTGTACAAATGTCT+TGG 0.528842 5.3:+1920235 MS.gene40493:CDS
CCTTGTCTTAACTATATCAC+AGG 0.529529 5.3:+1920269 MS.gene40493:CDS
AATCAAGCTGTCAATTCCGT+TGG 0.530988 5.3:+1920944 MS.gene40493:CDS
AAAAGAATAGCATTTCAAAT+GGG 0.533508 5.3:-1921033 None:intergenic
TCTCCAAACTCAGTTCCATC+AGG 0.538578 5.3:+1920766 MS.gene40493:CDS
GCATCCTGAAGACGGGGTTG+AGG 0.545184 5.3:-1920297 None:intergenic
ACAACCACTATGCCTATGTC+AGG 0.550990 5.3:+1920349 MS.gene40493:CDS
TCCACTGGATGATCCATCAC+CGG 0.557164 5.3:-1920916 None:intergenic
TTTGAGCAGCATCCTGAAGA+CGG 0.563609 5.3:-1920305 None:intergenic
GATGATCCATCACCGGTTGA+GGG 0.571557 5.3:-1920909 None:intergenic
TCCCCAGCAGACTCACCAGC+AGG 0.577866 5.3:+1920730 MS.gene40493:CDS
CTGCTCAAACCTTGCCAGTG+TGG 0.578926 5.3:+1920319 MS.gene40493:CDS
TGTTCAGAGAACTGAGGCAT+AGG 0.581130 5.3:-1920985 None:intergenic
GTGAATTCACCACACTGGCA+AGG 0.582240 5.3:-1920328 None:intergenic
GTAATCGCAATGTTGTGCGC+TGG 0.583284 5.3:+1920200 None:intergenic
TCCTGCTGGTGAGTCTGCTG+GGG 0.602035 5.3:-1920731 None:intergenic
AGAACCTGACATAGGCATAG+TGG 0.603929 5.3:-1920353 None:intergenic
AATCAGCATCAATCAAACTC+AGG 0.612879 5.3:+1920400 MS.gene40493:CDS
CTATGTCAGGTTCTCAGCGG+GGG 0.614715 5.3:+1920362 MS.gene40493:CDS
TGCCTATGTCAGGTTCTCAG+CGG 0.639245 5.3:+1920359 MS.gene40493:CDS
GCTGCCAAAATAAGAACCAA+CGG 0.650717 5.3:-1920960 None:intergenic
CCCCGCTGAGAACCTGACAT+AGG 0.652303 5.3:-1920361 None:intergenic
ACCGGTGATGGATCATCCAG+TGG 0.660594 5.3:+1920915 MS.gene40493:CDS
CCTATGTCAGGTTCTCAGCG+GGG 0.669708 5.3:+1920361 MS.gene40493:CDS
CCTGTGATATAGTTAAGACA+AGG 0.669942 5.3:-1920269 None:intergenic
CTTGTCTTAACTATATCACA+GGG 0.674749 5.3:+1920270 MS.gene40493:CDS
TGGTTGTGAATTCACCACAC+TGG 0.698930 5.3:-1920333 None:intergenic
TGAGCAGCATCCTGAAGACG+GGG 0.700847 5.3:-1920303 None:intergenic
TCAGTATGTTCAGAGAACTG+AGG 0.737979 5.3:-1920991 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATATAGTTTAGAATAT+GGG - chr5.3:1920672-1920691 None:intergenic 10.0%
!!! GAAATATATAGTTTAGAATA+TGG - chr5.3:1920673-1920692 None:intergenic 15.0%
!! CAAATGAATTTCAACTTAAA+TGG - chr5.3:1920866-1920885 None:intergenic 20.0%
! AGTCAGTGTAAAACTAATAA+TGG + chr5.3:1920467-1920486 MS.gene40493:intron 25.0%
! ATCTCAATGTTCATATAGTT+AGG - chr5.3:1920561-1920580 None:intergenic 25.0%
! TCTCAATGTTCATATAGTTA+GGG - chr5.3:1920560-1920579 None:intergenic 25.0%
! TTCAACTTAAATGGTTAACA+AGG - chr5.3:1920857-1920876 None:intergenic 25.0%
!!! ACTTATCTTTTTGAGTATCA+AGG - chr5.3:1920645-1920664 None:intergenic 25.0%
!!! ATACTGATTCTTTAATCAGT+TGG + chr5.3:1921007-1921026 MS.gene40493:CDS 25.0%
!!! ATTATTAGTTTTACACTGAC+TGG - chr5.3:1920468-1920487 None:intergenic 25.0%
!!! TACTGATTCTTTAATCAGTT+GGG + chr5.3:1921008-1921027 MS.gene40493:CDS 25.0%
!!! TTAAGTTGAAATTCATTTGC+AGG + chr5.3:1920867-1920886 MS.gene40493:intron 25.0%
AATGTTCATATAGTTAGGGT+TGG - chr5.3:1920556-1920575 None:intergenic 30.0%
CTTGTCTTAACTATATCACA+GGG + chr5.3:1920270-1920289 MS.gene40493:CDS 30.0%
TATAGTTAAGACAAGGTGAT+AGG - chr5.3:1920265-1920284 None:intergenic 30.0%
!!! AATTCCGTTGGTTCTTATTT+TGG + chr5.3:1920956-1920975 MS.gene40493:CDS 30.0%
AATCAGCATCAATCAAACTC+AGG + chr5.3:1920400-1920419 MS.gene40493:CDS 35.0%
CATCAGGTACTAACATATTG+GGG + chr5.3:1920782-1920801 MS.gene40493:intron 35.0%
CCATCAGGTACTAACATATT+GGG + chr5.3:1920781-1920800 MS.gene40493:intron 35.0%
CCTGTGATATAGTTAAGACA+AGG - chr5.3:1920272-1920291 None:intergenic 35.0%
CCTTGTCTTAACTATATCAC+AGG + chr5.3:1920269-1920288 MS.gene40493:CDS 35.0%
TCCATCAGGTACTAACATAT+TGG + chr5.3:1920780-1920799 MS.gene40493:intron 35.0%
TGAAATTCATTTGCAGGAAC+CGG + chr5.3:1920873-1920892 MS.gene40493:intron 35.0%
TGATCATCCAATTTGAACCT+TGG + chr5.3:1920831-1920850 MS.gene40493:intron 35.0%
TTAAATGGTTAACAAGGCCA+AGG - chr5.3:1920851-1920870 None:intergenic 35.0%
! ATCGAGTTGTACAAATGTCT+TGG + chr5.3:1920235-1920254 MS.gene40493:CDS 35.0%
!!! ATTAGTTTTACACTGACTGG+TGG - chr5.3:1920465-1920484 None:intergenic 35.0%
!!! TTAGTTTTACACTGACTGGT+GGG - chr5.3:1920464-1920483 None:intergenic 35.0%
AACAAGGCCAAGGTTCAAAT+TGG - chr5.3:1920841-1920860 None:intergenic 40.0%
AATCAAGCTGTCAATTCCGT+TGG + chr5.3:1920944-1920963 MS.gene40493:CDS 40.0%
CAGCTTGATTGAATTTCCAC+TGG - chr5.3:1920934-1920953 None:intergenic 40.0%
CCCAATATGTTAGTACCTGA+TGG - chr5.3:1920784-1920803 None:intergenic 40.0%
GCTGCCAAAATAAGAACCAA+CGG - chr5.3:1920963-1920982 None:intergenic 40.0%
TCAGTATGTTCAGAGAACTG+AGG - chr5.3:1920994-1921013 None:intergenic 40.0%
ACAACCACTATGCCTATGTC+AGG + chr5.3:1920349-1920368 MS.gene40493:CDS 45.0%
AGAACCTGACATAGGCATAG+TGG - chr5.3:1920356-1920375 None:intergenic 45.0%
GTACCTGATGGAACTGAGTT+TGG - chr5.3:1920772-1920791 None:intergenic 45.0%
TCAAAATCCACTCCCTCAAC+CGG + chr5.3:1920897-1920916 MS.gene40493:CDS 45.0%
TCTCCAAACTCAGTTCCATC+AGG + chr5.3:1920766-1920785 MS.gene40493:CDS 45.0%
TGGTTGTGAATTCACCACAC+TGG - chr5.3:1920336-1920355 None:intergenic 45.0%
TGTTCAGAGAACTGAGGCAT+AGG - chr5.3:1920988-1921007 None:intergenic 45.0%
TTTGAGCAGCATCCTGAAGA+CGG - chr5.3:1920308-1920327 None:intergenic 45.0%
!! TGAGGGAGTGGATTTTGATC+CGG - chr5.3:1920895-1920914 None:intergenic 45.0%
!! TTTTACACTGACTGGTGGGT+GGG - chr5.3:1920460-1920479 None:intergenic 45.0%
AATTCCTCAACCCCGTCTTC+AGG + chr5.3:1920293-1920312 MS.gene40493:CDS 50.0%
GTGAATTCACCACACTGGCA+AGG - chr5.3:1920331-1920350 None:intergenic 50.0%
TCCACTGGATGATCCATCAC+CGG - chr5.3:1920919-1920938 None:intergenic 50.0%
TGCCTATGTCAGGTTCTCAG+CGG + chr5.3:1920359-1920378 MS.gene40493:CDS 50.0%
TTGAGCAGCATCCTGAAGAC+GGG - chr5.3:1920307-1920326 None:intergenic 50.0%
! GATGATCCATCACCGGTTGA+GGG - chr5.3:1920912-1920931 None:intergenic 50.0%
! TTTACACTGACTGGTGGGTG+GGG - chr5.3:1920459-1920478 None:intergenic 50.0%
!! GTTTTACACTGACTGGTGGG+TGG - chr5.3:1920461-1920480 None:intergenic 50.0%
CCTATGTCAGGTTCTCAGCG+GGG + chr5.3:1920361-1920380 MS.gene40493:CDS 55.0%
CTATGTCAGGTTCTCAGCGG+GGG + chr5.3:1920362-1920381 MS.gene40493:CDS 55.0%
CTGCTCAAACCTTGCCAGTG+TGG + chr5.3:1920319-1920338 MS.gene40493:CDS 55.0%
GACTCTGCTTCTGTTCCTGC+TGG - chr5.3:1920748-1920767 None:intergenic 55.0%
GCCTATGTCAGGTTCTCAGC+GGG + chr5.3:1920360-1920379 MS.gene40493:CDS 55.0%
GTTTGCACCTTGCAAGCACC+AGG - chr5.3:1920437-1920456 None:intergenic 55.0%
TGAGCAGCATCCTGAAGACG+GGG - chr5.3:1920306-1920325 None:intergenic 55.0%
TTCCTGCTGGTGAGTCTGCT+GGG - chr5.3:1920735-1920754 None:intergenic 55.0%
! GGATGATCCATCACCGGTTG+AGG - chr5.3:1920913-1920932 None:intergenic 55.0%
!! ACCGGTGATGGATCATCCAG+TGG + chr5.3:1920915-1920934 MS.gene40493:CDS 55.0%
AGCCTTGCCTGGTGCTTGCA+AGG + chr5.3:1920427-1920446 MS.gene40493:CDS 60.0%
CACCTTGCAAGCACCAGGCA+AGG - chr5.3:1920432-1920451 None:intergenic 60.0%
CCCCGCTGAGAACCTGACAT+AGG - chr5.3:1920364-1920383 None:intergenic 60.0%
GCATCCTGAAGACGGGGTTG+AGG - chr5.3:1920300-1920319 None:intergenic 60.0%
GTTCCTGCTGGTGAGTCTGC+TGG - chr5.3:1920736-1920755 None:intergenic 60.0%
TCCACTCCCTCAACCGGTGA+TGG + chr5.3:1920903-1920922 MS.gene40493:CDS 60.0%
TCCTGCTGGTGAGTCTGCTG+GGG - chr5.3:1920734-1920753 None:intergenic 60.0%
! TCCATCACCGGTTGAGGGAG+TGG - chr5.3:1920907-1920926 None:intergenic 60.0%
!! ACTCAGGCTCTAGCCTTGCC+TGG + chr5.3:1920416-1920435 MS.gene40493:CDS 60.0%
TCCCCAGCAGACTCACCAGC+AGG + chr5.3:1920730-1920749 MS.gene40493:CDS 65.0%
! AGCGGGGGTGCATCGTCGCT+GGG + chr5.3:1920377-1920396 MS.gene40493:CDS 70.0%
! CAGCGGGGGTGCATCGTCGC+TGG + chr5.3:1920376-1920395 MS.gene40493:CDS 75.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 1920209 1921041 1920209 ID=MS.gene40493
chr5.3 mRNA 1920209 1921041 1920209 ID=MS.gene40493.t1;Parent=MS.gene40493
chr5.3 exon 1920209 1920448 1920209 ID=MS.gene40493.t1.exon1;Parent=MS.gene40493.t1
chr5.3 CDS 1920209 1920448 1920209 ID=cds.MS.gene40493.t1;Parent=MS.gene40493.t1
chr5.3 exon 1920725 1920787 1920725 ID=MS.gene40493.t1.exon2;Parent=MS.gene40493.t1
chr5.3 CDS 1920725 1920787 1920725 ID=cds.MS.gene40493.t1;Parent=MS.gene40493.t1
chr5.3 exon 1920889 1921041 1920889 ID=MS.gene40493.t1.exon3;Parent=MS.gene40493.t1
chr5.3 CDS 1920889 1921041 1920889 ID=cds.MS.gene40493.t1;Parent=MS.gene40493.t1
Gene Sequence

>MS.gene40493

ATGTTGTGCGCTGGAGCTGCTGCGCAATCGAGTTGTACAAATGTCTTGGTCAGCCTATCACCTTGTCTTAACTATATCACAGGGAATTCCTCAACCCCGTCTTCAGGATGCTGCTCAAACCTTGCCAGTGTGGTGAATTCACAACCACTATGCCTATGTCAGGTTCTCAGCGGGGGTGCATCGTCGCTGGGAATCAGCATCAATCAAACTCAGGCTCTAGCCTTGCCTGGTGCTTGCAAGGTGCAAACCCCACCCACCAGTCAGTGTAAAACTAATAATGGTAAGAGAACAAAATTTCTATTTGATTGAGTTGAGAAAAAAATCAGCTCGACAAATATGTGCATCCAACCCTAACTATATGAACATTGAGATATATACTATGCAAATATCTATCATTATACTATAGAGACAGTGAATAGAAACTATAAGAGAACCTTGATACTCAAAAAGATAAGTACAGCCCATATTCTAAACTATATATTTCTGCTAACTTACAATATATGTTTTTGTCAACAGCTGCTTCCCCAGCAGACTCACCAGCAGGAACAGAAGCAGAGTCTCCAAACTCAGTTCCATCAGGTACTAACATATTGGGGAAGTAATTTAGTTATGCTTCAGAGCTTGATCATCCAATTTGAACCTTGGCCTTGTTAACCATTTAAGTTGAAATTCATTTGCAGGAACCGGATCAAAATCCACTCCCTCAACCGGTGATGGATCATCCAGTGGAAATTCAATCAAGCTGTCAATTCCGTTGGTTCTTATTTTGGCAGCAGCCTATGCCTCAGTTCTCTGAACATACTGATTCTTTAATCAGTTGGGTTCCCATTTGA

Protein sequence

>MS.gene40493.t1

MLCAGAAAQSSCTNVLVSLSPCLNYITGNSSTPSSGCCSNLASVVNSQPLCLCQVLSGGASSLGISINQTQALALPGACKLLPQQTHQQEQKQSLQTQFHQEPDQNPLPQPVMDHPVEIQSSCQFRWFLFWQQPMPQFSEHTDSLISWVPI