AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44893


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44893.t1 MTR_5g070210 84.706 170 19 2 1 170 1 163 9.69e-102 295
MS.gene44893.t1 MTR_5g070230 60.000 130 50 1 45 172 3 132 3.11e-53 168
MS.gene44893.t1 MTR_5g070270 48.413 126 44 7 1 115 1 116 4.32e-24 91.7
MS.gene44893.t1 MTR_5g070250 59.677 62 24 1 113 173 20 81 1.11e-19 82.8
MS.gene44893.t1 MTR_8g073540 46.753 77 41 0 85 161 33 109 2.00e-17 78.6
MS.gene44893.t1 MTR_1g095500 45.455 77 42 0 85 161 33 109 2.75e-17 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44893.t1 AT5G58770 63.441 93 34 0 81 173 73 165 2.77e-40 138
MS.gene44893.t1 AT5G58782 53.000 100 47 0 74 173 46 145 2.73e-30 112
MS.gene44893.t1 AT5G58782 53.000 100 47 0 74 173 64 163 3.10e-30 112
MS.gene44893.t1 AT5G58780 55.914 93 41 0 78 170 63 155 3.90e-29 109
MS.gene44893.t1 AT2G23400 41.085 129 75 1 45 173 31 158 2.76e-28 104
MS.gene44893.t1 AT2G23400 41.085 129 75 1 45 173 31 158 3.28e-27 103
MS.gene44893.t1 AT2G23410 49.474 95 47 1 80 173 65 159 6.89e-25 98.2
MS.gene44893.t1 AT5G58784 48.889 90 46 0 84 173 69 158 1.83e-24 97.1
MS.gene44893.t1 AT2G17570 47.561 82 43 0 85 166 39 120 1.21e-18 81.3

Find 49 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCATTCTCAACCGATAAT+TGG 0.274745 5.2:-59377331 MS.gene44893:CDS
TAAGCATGTGGCGGTGATTA+TGG 0.306230 5.2:-59377480 MS.gene44893:CDS
GTGTTATAGTTGGGGAATAA+AGG 0.324367 5.2:-59377366 MS.gene44893:CDS
CTCATTCTCGATATTCTTCA+AGG 0.331892 5.2:-59377628 MS.gene44893:CDS
TTAGTAGTAGTAGTTGTCTT+TGG 0.362349 5.2:+59377683 None:intergenic
ATGGGAGGTGGGCAAAGATG+AGG 0.382978 5.2:-59377451 MS.gene44893:CDS
TACTACTAACCATGTTCATC+GGG 0.399960 5.2:-59377669 MS.gene44893:CDS
GGTGAGGTTGTGTTATAGTT+GGG 0.400487 5.2:-59377375 MS.gene44893:CDS
TTCAGAAATAGAGCCATTGC+CGG 0.414839 5.2:-59377528 MS.gene44893:CDS
CTTAGGTCGAATCCAATTAT+CGG 0.425110 5.2:+59377319 None:intergenic
GTAGTAGTAGTTGTCTTTGG+TGG 0.450585 5.2:+59377686 None:intergenic
TGGTGAGGTTGTGTTATAGT+TGG 0.451686 5.2:-59377376 MS.gene44893:CDS
CGGTGATTATGGACGGGAAT+GGG 0.455731 5.2:-59377469 MS.gene44893:CDS
TGGGAGGTGGGCAAAGATGA+GGG 0.455809 5.2:-59377450 MS.gene44893:CDS
GGGAGGTGGGCAAAGATGAG+GGG 0.456021 5.2:-59377449 MS.gene44893:CDS
GGAGGTGGGCAAAGATGAGG+GGG 0.479875 5.2:-59377448 MS.gene44893:CDS
CGATAATTGGATTCGACCTA+AGG 0.480618 5.2:-59377318 MS.gene44893:intron
ATTGCCGGAGGAACTGCAAT+CGG 0.484701 5.2:-59377513 MS.gene44893:CDS
AGCTCCGATTGCAGTTCCTC+CGG 0.485552 5.2:+59377509 None:intergenic
CGCGGCAATAGTGACGCGTT+TGG 0.485624 5.2:+59377598 None:intergenic
CCGTCTGCGGGTCATGTAGC+CGG 0.489572 5.2:-59377419 MS.gene44893:CDS
TTATGGACGGGAATGGGAGG+TGG 0.493196 5.2:-59377463 MS.gene44893:CDS
GCGGTGATTATGGACGGGAA+TGG 0.517607 5.2:-59377470 MS.gene44893:CDS
CTACTACTAACCATGTTCAT+CGG 0.518028 5.2:-59377670 MS.gene44893:CDS
GATTGCAGTTCCTCCGGCAA+TGG 0.522861 5.2:+59377515 None:intergenic
CGGCTACATGACCCGCAGAC+GGG 0.532986 5.2:+59377420 None:intergenic
AATTTATTGTTCTTTCAAAG+AGG 0.535873 5.2:+59377165 None:intergenic
GTGAGGTTGTGTTATAGTTG+GGG 0.555675 5.2:-59377374 MS.gene44893:CDS
ATAATCACCGCCACATGCTT+AGG 0.560502 5.2:+59377482 None:intergenic
TATGGACGGGAATGGGAGGT+GGG 0.565954 5.2:-59377462 MS.gene44893:CDS
ATGTGGCGGTGATTATGGAC+GGG 0.576395 5.2:-59377475 MS.gene44893:CDS
TGTAAAGTCGTTGAGAAGGA+TGG 0.579930 5.2:-59377396 MS.gene44893:CDS
GGCTACATGACCCGCAGACG+GGG 0.586275 5.2:+59377421 None:intergenic
CATGTGGCGGTGATTATGGA+CGG 0.587809 5.2:-59377476 MS.gene44893:CDS
CTGAAATTGAAGCACTAGAG+AGG 0.594486 5.2:-59377142 MS.gene44893:CDS
TGATTATGGACGGGAATGGG+AGG 0.615382 5.2:-59377466 MS.gene44893:CDS
CCGGCTACATGACCCGCAGA+CGG 0.616650 5.2:+59377419 None:intergenic
CAAACGCGTCACTATTGCCG+CGG 0.621205 5.2:-59377597 MS.gene44893:CDS
CCGGTGTAAAGTCGTTGAGA+AGG 0.624155 5.2:-59377400 MS.gene44893:CDS
AGTCGTTGAGAAGGATGGTG+AGG 0.625194 5.2:-59377391 MS.gene44893:CDS
GGTGGATGGGGAAACTTCCG+TGG 0.636917 5.2:-59377561 MS.gene44893:CDS
CCTTCTCAACGACTTTACAC+CGG 0.638232 5.2:+59377400 None:intergenic
AGAAATAGAGCCATTGCCGG+AGG 0.640104 5.2:-59377525 MS.gene44893:CDS
CACCTGATCCTGAAAAGCCG+CGG 0.664185 5.2:+59377580 None:intergenic
GGAGCTGATGCCTAAGCATG+TGG 0.668799 5.2:-59377492 MS.gene44893:CDS
GCTACATGACCCGCAGACGG+GGG 0.669075 5.2:+59377422 None:intergenic
CTACTAACCATGTTCATCGG+GGG 0.676277 5.2:-59377667 MS.gene44893:CDS
ACTACTAACCATGTTCATCG+GGG 0.699404 5.2:-59377668 MS.gene44893:CDS
GCTGATGCCTAAGCATGTGG+CGG 0.712093 5.2:-59377489 MS.gene44893:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTCTTAGTTTTGATTAT+TGG - chr5.2:59377619-59377638 MS.gene44893:CDS 15.0%
!! AATTTATTGTTCTTTCAAAG+AGG + chr5.2:59377687-59377706 None:intergenic 20.0%
! AGTGAAATTAAAATGACCTT+AGG + chr5.2:59377550-59377569 None:intergenic 25.0%
! ATGACATTAGTTATTGATAG+TGG - chr5.2:59377596-59377615 MS.gene44893:CDS 25.0%
!!! GTTATAAGAGTTTTATCTAG+TGG + chr5.2:59377139-59377158 None:intergenic 25.0%
TTAGTAGTAGTAGTTGTCTT+TGG + chr5.2:59377169-59377188 None:intergenic 30.0%
!!! GTTTTGATTATTGGTGTTAC+TGG - chr5.2:59377628-59377647 MS.gene44893:CDS 30.0%
CTACTACTAACCATGTTCAT+CGG - chr5.2:59377179-59377198 MS.gene44893:CDS 35.0%
CTCATTCTCGATATTCTTCA+AGG - chr5.2:59377221-59377240 MS.gene44893:intron 35.0%
CTTAGGTCGAATCCAATTAT+CGG + chr5.2:59377533-59377552 None:intergenic 35.0%
GTGTTATAGTTGGGGAATAA+AGG - chr5.2:59377483-59377502 MS.gene44893:CDS 35.0%
TACTACTAACCATGTTCATC+GGG - chr5.2:59377180-59377199 MS.gene44893:CDS 35.0%
TTGCATTCTCAACCGATAAT+TGG - chr5.2:59377518-59377537 MS.gene44893:CDS 35.0%
! TTCTGAAAAATCTTCGTCCA+CGG + chr5.2:59377308-59377327 None:intergenic 35.0%
!! AAGAGTTTTATCTAGTGGCA+TGG + chr5.2:59377134-59377153 None:intergenic 35.0%
!! AGAGTTTTATCTAGTGGCAT+GGG + chr5.2:59377133-59377152 None:intergenic 35.0%
ACTACTAACCATGTTCATCG+GGG - chr5.2:59377181-59377200 MS.gene44893:CDS 40.0%
CGATAATTGGATTCGACCTA+AGG - chr5.2:59377531-59377550 MS.gene44893:CDS 40.0%
GGTGAGGTTGTGTTATAGTT+GGG - chr5.2:59377474-59377493 MS.gene44893:CDS 40.0%
GTAGTAGTAGTTGTCTTTGG+TGG + chr5.2:59377166-59377185 None:intergenic 40.0%
GTGAGGTTGTGTTATAGTTG+GGG - chr5.2:59377475-59377494 MS.gene44893:CDS 40.0%
TGGTGAGGTTGTGTTATAGT+TGG - chr5.2:59377473-59377492 MS.gene44893:CDS 40.0%
TGTAAAGTCGTTGAGAAGGA+TGG - chr5.2:59377453-59377472 MS.gene44893:CDS 40.0%
TTCAGAAATAGAGCCATTGC+CGG - chr5.2:59377321-59377340 MS.gene44893:CDS 40.0%
! CTGAAATTGAAGCACTAGAG+AGG - chr5.2:59377707-59377726 MS.gene44893:CDS 40.0%
ATAATCACCGCCACATGCTT+AGG + chr5.2:59377370-59377389 None:intergenic 45.0%
CCTTCTCAACGACTTTACAC+CGG + chr5.2:59377452-59377471 None:intergenic 45.0%
CTACTAACCATGTTCATCGG+GGG - chr5.2:59377182-59377201 MS.gene44893:CDS 45.0%
TAAGCATGTGGCGGTGATTA+TGG - chr5.2:59377369-59377388 MS.gene44893:CDS 45.0%
TGTTACTGGTGATATGCAGG+TGG - chr5.2:59377642-59377661 MS.gene44893:CDS 45.0%
!! CTTTTCAGGATCAGGTGGAT+GGG - chr5.2:59377275-59377294 MS.gene44893:intron 45.0%
!! TGGTGTTACTGGTGATATGC+AGG - chr5.2:59377639-59377658 MS.gene44893:CDS 45.0%
!! TTTTCAGGATCAGGTGGATG+GGG - chr5.2:59377276-59377295 MS.gene44893:intron 45.0%
AGAAATAGAGCCATTGCCGG+AGG - chr5.2:59377324-59377343 MS.gene44893:CDS 50.0%
AGTCGTTGAGAAGGATGGTG+AGG - chr5.2:59377458-59377477 MS.gene44893:CDS 50.0%
ATGTGGCGGTGATTATGGAC+GGG - chr5.2:59377374-59377393 MS.gene44893:CDS 50.0%
ATTGCCGGAGGAACTGCAAT+CGG - chr5.2:59377336-59377355 MS.gene44893:CDS 50.0%
CATGTGGCGGTGATTATGGA+CGG - chr5.2:59377373-59377392 MS.gene44893:CDS 50.0%
CCGGTGTAAAGTCGTTGAGA+AGG - chr5.2:59377449-59377468 MS.gene44893:CDS 50.0%
CGGTGATTATGGACGGGAAT+GGG - chr5.2:59377380-59377399 MS.gene44893:CDS 50.0%
TGATTATGGACGGGAATGGG+AGG - chr5.2:59377383-59377402 MS.gene44893:CDS 50.0%
!! GCTTTTCAGGATCAGGTGGA+TGG - chr5.2:59377274-59377293 MS.gene44893:intron 50.0%
AGCTCCGATTGCAGTTCCTC+CGG + chr5.2:59377343-59377362 None:intergenic 55.0%
ATGGGAGGTGGGCAAAGATG+AGG - chr5.2:59377398-59377417 MS.gene44893:CDS 55.0%
CAAACGCGTCACTATTGCCG+CGG - chr5.2:59377252-59377271 MS.gene44893:intron 55.0%
CACCTGATCCTGAAAAGCCG+CGG + chr5.2:59377272-59377291 None:intergenic 55.0%
GAGTGATCCCCCGATGAACA+TGG + chr5.2:59377192-59377211 None:intergenic 55.0%
GATTGCAGTTCCTCCGGCAA+TGG + chr5.2:59377337-59377356 None:intergenic 55.0%
GCGGTGATTATGGACGGGAA+TGG - chr5.2:59377379-59377398 MS.gene44893:CDS 55.0%
GCTGATGCCTAAGCATGTGG+CGG - chr5.2:59377360-59377379 MS.gene44893:CDS 55.0%
GGAGCTGATGCCTAAGCATG+TGG - chr5.2:59377357-59377376 MS.gene44893:CDS 55.0%
TATGGACGGGAATGGGAGGT+GGG - chr5.2:59377387-59377406 MS.gene44893:CDS 55.0%
TGGGAGGTGGGCAAAGATGA+GGG - chr5.2:59377399-59377418 MS.gene44893:CDS 55.0%
TTATGGACGGGAATGGGAGG+TGG - chr5.2:59377386-59377405 MS.gene44893:CDS 55.0%
! CACTATTGCCGCGGCTTTTC+AGG - chr5.2:59377261-59377280 MS.gene44893:intron 55.0%
CGCGGCAATAGTGACGCGTT+TGG + chr5.2:59377254-59377273 None:intergenic 60.0%
GGAGGTGGGCAAAGATGAGG+GGG - chr5.2:59377401-59377420 MS.gene44893:CDS 60.0%
GGGAGGTGGGCAAAGATGAG+GGG - chr5.2:59377400-59377419 MS.gene44893:CDS 60.0%
GGTGGATGGGGAAACTTCCG+TGG - chr5.2:59377288-59377307 MS.gene44893:intron 60.0%
!! CGCGGCTTTTCAGGATCAGG+TGG - chr5.2:59377270-59377289 MS.gene44893:intron 60.0%
!! TGCCGCGGCTTTTCAGGATC+AGG - chr5.2:59377267-59377286 MS.gene44893:intron 60.0%
CCGGCTACATGACCCGCAGA+CGG + chr5.2:59377433-59377452 None:intergenic 65.0%
CCGTCTGCGGGTCATGTAGC+CGG - chr5.2:59377430-59377449 MS.gene44893:CDS 65.0%
CGGCTACATGACCCGCAGAC+GGG + chr5.2:59377432-59377451 None:intergenic 65.0%
GCTACATGACCCGCAGACGG+GGG + chr5.2:59377430-59377449 None:intergenic 65.0%
GGCTACATGACCCGCAGACG+GGG + chr5.2:59377431-59377450 None:intergenic 65.0%
AGGGGGTTACCCCCGTCTGC+GGG - chr5.2:59377418-59377437 MS.gene44893:CDS 70.0%
GAGGGGGTTACCCCCGTCTG+CGG - chr5.2:59377417-59377436 MS.gene44893:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 59377112 59377759 59377112 ID=MS.gene44893
chr5.2 mRNA 59377112 59377759 59377112 ID=MS.gene44893.t1;Parent=MS.gene44893
chr5.2 exon 59377319 59377759 59377319 ID=MS.gene44893.t1.exon1;Parent=MS.gene44893.t1
chr5.2 CDS 59377319 59377759 59377319 ID=cds.MS.gene44893.t1;Parent=MS.gene44893.t1
chr5.2 exon 59377112 59377210 59377112 ID=MS.gene44893.t1.exon2;Parent=MS.gene44893.t1
chr5.2 CDS 59377112 59377210 59377112 ID=cds.MS.gene44893.t1;Parent=MS.gene44893.t1
Gene Sequence

>MS.gene44893

ATGTTTTCGTTAAGACTTCCCATGCCACTAGATAAAACTCTTATAACTTCTCCACCAAAGACAACTACTACTACTAACCATGTTCATCGGGGGATCACTCAGATACAAACTCATTCTCGATATTCTTCAAGGTTTGTTACCAAACGCGTCACTATTGCCGCGGCTTTTCAGGATCAGGTGGATGGGGAAACTTCCGTGGACGAAGATTTTTCAGAAATAGAGCCATTGCCGGAGGAACTGCAATCGGAGCTGATGCCTAAGCATGTGGCGGTGATTATGGACGGGAATGGGAGGTGGGCAAAGATGAGGGGGTTACCCCCGTCTGCGGGTCATGTAGCCGGTGTAAAGTCGTTGAGAAGGATGGTGAGGTTGTGTTATAGTTGGGGAATAAAGGTTCTAACTGTTTTTGCATTCTCAACCGATAATTGGATTCGACCTAAGGTCATTTTAATTTCACTTGTTAGTTTAGTTCACTTTGATTAACATGACATTAGTTATTGATAGTGGTTATTCTTAGTTTTGATTATTGGTGTTACTGGTGATATGCAGGTGGAAGTTGAATTTTTGATGAGCCTCTTTGAAAGAACAATAAATTCTGAAATTGAAGCACTAGAGAGGTGTCAAATTCTAACTGCTTTAACTAATTAA

Protein sequence

>MS.gene44893.t1

MFSLRLPMPLDKTLITSPPKTTTTTNHVHRGITQIQTHSRYSSRFVTKRVTIAAAFQDQVDGETSVDEDFSEIEPLPEELQSELMPKHVAVIMDGNGRWAKMRGLPPSAGHVAGVKSLRRMVRLCYSWGIKVLTVFAFSTDNWIRPKVEVEFLMSLFERTINSEIEALERCQILTALTN