Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46387.t1 | XP_002526487.1 | 100 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 1.30E-43 | 185.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46387.t1 | Q9XHE4 | 97.8 | 92 | 2 | 0 | 1 | 92 | 1 | 92 | 5.9e-45 | 181.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46387.t1 | A0A251S2J9 | 100.0 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 9.4e-44 | 185.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050134 | MS.gene46387 | 0.875059 | 4.15E-68 | -1.69E-46 |
| MS.gene050252 | MS.gene46387 | 0.827847 | 1.25E-54 | -1.69E-46 |
| MS.gene050791 | MS.gene46387 | 0.877974 | 4.08E-69 | -1.69E-46 |
| MS.gene050912 | MS.gene46387 | 0.856767 | 2.58E-62 | -1.69E-46 |
| MS.gene051904 | MS.gene46387 | 0.874639 | 5.77E-68 | -1.69E-46 |
| MS.gene051984 | MS.gene46387 | 0.847278 | 1.28E-59 | -1.69E-46 |
| MS.gene052664 | MS.gene46387 | 0.874874 | 4.80E-68 | -1.69E-46 |
| MS.gene053224 | MS.gene46387 | 0.853402 | 2.45E-61 | -1.69E-46 |
| MS.gene056917 | MS.gene46387 | 0.812901 | 3.36E-51 | -1.69E-46 |
| MS.gene057092 | MS.gene46387 | 0.828368 | 9.35E-55 | -1.69E-46 |
| MS.gene057235 | MS.gene46387 | 0.826709 | 2.34E-54 | -1.69E-46 |
| MS.gene057272 | MS.gene46387 | 0.863737 | 2.01E-64 | -1.69E-46 |
| MS.gene057641 | MS.gene46387 | 0.853491 | 2.31E-61 | -1.69E-46 |
| MS.gene057643 | MS.gene46387 | 0.874418 | 6.86E-68 | -1.69E-46 |
| MS.gene059340 | MS.gene46387 | 0.80038 | 1.48E-48 | -1.69E-46 |
| MS.gene060483 | MS.gene46387 | 0.823512 | 1.33E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46387.t1 | MTR_4g099160 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 9.47e-63 | 185 |
| MS.gene46387.t1 | MTR_4g103340 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 9.47e-63 | 185 |
| MS.gene46387.t1 | MTR_8g007485 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 9.47e-63 | 185 |
| MS.gene46387.t1 | MTR_0199s0040 | 100.000 | 92 | 0 | 0 | 1 | 92 | 82 | 173 | 1.46e-62 | 187 |
| MS.gene46387.t1 | MTR_4g103340 | 100.000 | 71 | 0 | 0 | 1 | 71 | 1 | 71 | 9.22e-47 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46387.t1 | AT3G10950 | 93.478 | 92 | 6 | 0 | 1 | 92 | 1 | 92 | 4.32e-59 | 176 |
| MS.gene46387.t1 | AT3G60245 | 92.308 | 91 | 7 | 0 | 1 | 91 | 1 | 91 | 8.36e-58 | 172 |
Find 23 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAAAGGCTGTAGGAATA+TGG | 0.316747 | 8.4:-23834952 | MS.gene46387:CDS |
| GAGAAAGGCTGTAGGAATAT+GGG | 0.416937 | 8.4:-23834951 | MS.gene46387:CDS |
| GCTGGTATCGTTGGCAAATA+CGG | 0.433650 | 8.4:-23835847 | MS.gene46387:intron |
| ATGATATTAGGTACCCGTTA+TGG | 0.441488 | 8.4:-23835175 | MS.gene46387:intron |
| CTGCAAGTGCCGTGACTGTT+CGG | 0.445396 | 8.4:-23834792 | MS.gene46387:CDS |
| TGACTGTTCGGAGCACCATC+AGG | 0.472923 | 8.4:-23834780 | MS.gene46387:CDS |
| ACGCAAACTAGCACCATAAC+GGG | 0.476774 | 8.4:+23835162 | None:intergenic |
| TTGTTCCCTCAACCTCCTGA+TGG | 0.500191 | 8.4:+23834765 | None:intergenic |
| GGAGCACCATCAGGAGGTTG+AGG | 0.503709 | 8.4:-23834771 | MS.gene46387:CDS |
| TACGCAAACTAGCACCATAA+CGG | 0.535734 | 8.4:+23835161 | None:intergenic |
| AGAAAGGCTGTAGGAATATG+GGG | 0.537082 | 8.4:-23834950 | MS.gene46387:CDS |
| ACTAAGAGAACTAAGAAGGC+TGG | 0.546098 | 8.4:-23835865 | MS.gene46387:CDS |
| GAGCACCATCAGGAGGTTGA+GGG | 0.548544 | 8.4:-23834770 | MS.gene46387:CDS |
| ACTAAGAAGGCTGGTATCGT+TGG | 0.554051 | 8.4:-23835856 | MS.gene46387:CDS |
| GCAGACTAAGAGAACTAAGA+AGG | 0.566891 | 8.4:-23835869 | MS.gene46387:intron |
| GATTGCGGTAAAGTGAAAGC+AGG | 0.587249 | 8.4:-23834920 | MS.gene46387:CDS |
| TGTAGGAATATGGGGATGCA+AGG | 0.599232 | 8.4:-23834942 | MS.gene46387:CDS |
| GATGGTGCTCCGAACAGTCA+CGG | 0.619064 | 8.4:+23834783 | None:intergenic |
| GCAGTATGCTGTGAAGAGAA+AGG | 0.632531 | 8.4:-23834966 | MS.gene46387:intron |
| TGCGGTAAAGTGAAAGCAGG+CGG | 0.647382 | 8.4:-23834917 | MS.gene46387:CDS |
| ATATGGGGATGCAAGGATTG+CGG | 0.650089 | 8.4:-23834935 | MS.gene46387:CDS |
| GCTGTGAAGAGAAAGGCTGT+AGG | 0.663437 | 8.4:-23834959 | MS.gene46387:CDS |
| CTGTTCGGAGCACCATCAGG+AGG | 0.673364 | 8.4:-23834777 | MS.gene46387:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAAATAATATTTTATGCA+AGG | - | chr8.4:23835322-23835341 | MS.gene46387:intron | 10.0% |
| !!! | ATTATATTTTATGTAAAACA+AGG | + | chr8.4:23835553-23835572 | None:intergenic | 10.0% |
| !!! | TTTACTAAATGTTTTAAATA+TGG | - | chr8.4:23835070-23835089 | MS.gene46387:intron | 10.0% |
| !! | TATACATAGATATATGTATA+TGG | - | chr8.4:23835207-23835226 | MS.gene46387:intron | 15.0% |
| !!! | TTTTATTTTTCAAATCTGAA+AGG | - | chr8.4:23835963-23835982 | MS.gene46387:intron | 15.0% |
| !! | ACAAAGAATTTGTAAGATTT+CGG | + | chr8.4:23834999-23835018 | None:intergenic | 20.0% |
| !! | ACAAATACAATTCATGAATT+GGG | + | chr8.4:23835519-23835538 | None:intergenic | 20.0% |
| !! | GAAAAATAAAACATTGAGAA+AGG | + | chr8.4:23835954-23835973 | None:intergenic | 20.0% |
| !! | TACAAATACAATTCATGAAT+TGG | + | chr8.4:23835520-23835539 | None:intergenic | 20.0% |
| !! | TTTATGAGAAGTTAATTCAT+TGG | - | chr8.4:23835608-23835627 | MS.gene46387:intron | 20.0% |
| !!! | AAATATGGTTTTGTTTATGA+TGG | - | chr8.4:23835085-23835104 | MS.gene46387:intron | 20.0% |
| !!! | AAATTTTTCTGTGAATTTTG+TGG | - | chr8.4:23835712-23835731 | MS.gene46387:intron | 20.0% |
| !!! | ATCTAAGTATTTAGTTTAGT+TGG | - | chr8.4:23835776-23835795 | MS.gene46387:intron | 20.0% |
| !!! | TGGTTTTATTGTATGATATT+AGG | - | chr8.4:23835628-23835647 | MS.gene46387:intron | 20.0% |
| ! | AAAACATTGAGAAAGGAATA+AGG | + | chr8.4:23835947-23835966 | None:intergenic | 25.0% |
| ! | AAAATAAACCAATCCAAACT+AGG | + | chr8.4:23835276-23835295 | None:intergenic | 25.0% |
| ! | ATGATATACAACATTACACA+AGG | + | chr8.4:23835747-23835766 | None:intergenic | 25.0% |
| ! | GATTTATTTGTAACTCTTCA+AGG | - | chr8.4:23835576-23835595 | MS.gene46387:intron | 25.0% |
| !! | TTGAAAGAGAAGTTTAGAAT+TGG | + | chr8.4:23834806-23834825 | None:intergenic | 25.0% |
| !!! | TTGGTTTATTTTTGAGTTTG+TGG | - | chr8.4:23835284-23835303 | MS.gene46387:intron | 25.0% |
| ACCTAAAATGTAACTACCTA+GGG | + | chr8.4:23835454-23835473 | None:intergenic | 30.0% | |
| ATAATGAGAACAAAGTCATG+AGG | + | chr8.4:23835993-23836012 | None:intergenic | 30.0% | |
| TTACAAATCTGAGTGAACAA+AGG | + | chr8.4:23834849-23834868 | None:intergenic | 30.0% | |
| ! | AGAGAAGTTTAGAATTGGAT+TGG | + | chr8.4:23834801-23834820 | None:intergenic | 30.0% |
| ! | TTTAGAATTGGATTGGATGA+TGG | + | chr8.4:23834794-23834813 | None:intergenic | 30.0% |
| ! | TTTAGGTGGTTTGATGTTTT+GGG | - | chr8.4:23835467-23835486 | MS.gene46387:intron | 30.0% |
| ! | TTTCTGTGAATTTTGTGGAA+AGG | - | chr8.4:23835717-23835736 | MS.gene46387:intron | 30.0% |
| !! | TTTTAGGTGGTTTGATGTTT+TGG | - | chr8.4:23835466-23835485 | MS.gene46387:intron | 30.0% |
| !!! | TAAGCTATTTTTCATAAGCC+AGG | - | chr8.4:23835428-23835447 | MS.gene46387:intron | 30.0% |
| ATCTGAGTGAACAAAGGATT+CGG | + | chr8.4:23834843-23834862 | None:intergenic | 35.0% | |
| ATGATATTAGGTACCCGTTA+TGG | - | chr8.4:23835640-23835659 | MS.gene46387:intron | 35.0% | |
| CACCTAAAATGTAACTACCT+AGG | + | chr8.4:23835455-23835474 | None:intergenic | 35.0% | |
| GCGTAAGCAGATTAAAAAGA+TGG | - | chr8.4:23835672-23835691 | MS.gene46387:intron | 35.0% | |
| GGTAATTGATCATCAGAACT+CGG | + | chr8.4:23835825-23835844 | None:intergenic | 35.0% | |
| ! | CTAGGTAGTTACATTTTAGG+TGG | - | chr8.4:23835453-23835472 | MS.gene46387:intron | 35.0% |
| ! | TTAGGTGGTTTGATGTTTTG+GGG | - | chr8.4:23835468-23835487 | MS.gene46387:intron | 35.0% |
| !! | ATTGGATTGGATGATGGAAA+AGG | + | chr8.4:23834788-23834807 | None:intergenic | 35.0% |
| !! | TTGGATTGGATGATGGAAAA+GGG | + | chr8.4:23834787-23834806 | None:intergenic | 35.0% |
| ACTAAGAGAACTAAGAAGGC+TGG | - | chr8.4:23834950-23834969 | MS.gene46387:CDS | 40.0% | |
| AGAAAGGCTGTAGGAATATG+GGG | - | chr8.4:23835865-23835884 | MS.gene46387:CDS | 40.0% | |
| AGAGAAAGGCTGTAGGAATA+TGG | - | chr8.4:23835863-23835882 | MS.gene46387:CDS | 40.0% | |
| GAGAAAGGCTGTAGGAATAT+GGG | - | chr8.4:23835864-23835883 | MS.gene46387:CDS | 40.0% | |
| GCAGACTAAGAGAACTAAGA+AGG | - | chr8.4:23834946-23834965 | MS.gene46387:CDS | 40.0% | |
| TACGCAAACTAGCACCATAA+CGG | + | chr8.4:23835657-23835676 | None:intergenic | 40.0% | |
| TGACTACACCTAGTTTGGAT+TGG | - | chr8.4:23835265-23835284 | MS.gene46387:intron | 40.0% | |
| ! | GCCCTAGGTAGTTACATTTT+AGG | - | chr8.4:23835450-23835469 | MS.gene46387:intron | 40.0% |
| !! | CAGAACTCGGCATTTTGAAA+AGG | + | chr8.4:23835812-23835831 | None:intergenic | 40.0% |
| !! | TAGGTGGTTTGATGTTTTGG+GGG | - | chr8.4:23835469-23835488 | MS.gene46387:intron | 40.0% |
| !! | TGGATTGGATGATGGAAAAG+GGG | + | chr8.4:23834786-23834805 | None:intergenic | 40.0% |
| AAATGTAACTACCTAGGGCC+TGG | + | chr8.4:23835449-23835468 | None:intergenic | 45.0% | |
| ACGCAAACTAGCACCATAAC+GGG | + | chr8.4:23835656-23835675 | None:intergenic | 45.0% | |
| ACTAAGAAGGCTGGTATCGT+TGG | - | chr8.4:23834959-23834978 | MS.gene46387:CDS | 45.0% | |
| ATATGGGGATGCAAGGATTG+CGG | - | chr8.4:23835880-23835899 | MS.gene46387:intron | 45.0% | |
| GATTGCGGTAAAGTGAAAGC+AGG | - | chr8.4:23835895-23835914 | MS.gene46387:intron | 45.0% | |
| GCAGTATGCTGTGAAGAGAA+AGG | - | chr8.4:23835849-23835868 | MS.gene46387:CDS | 45.0% | |
| GCTGGTATCGTTGGCAAATA+CGG | - | chr8.4:23834968-23834987 | MS.gene46387:intron | 45.0% | |
| GTAGCTGACTACACCTAGTT+TGG | - | chr8.4:23835260-23835279 | MS.gene46387:intron | 45.0% | |
| TCTTCACAGCATACTGCAAC+AGG | + | chr8.4:23835846-23835865 | None:intergenic | 45.0% | |
| TGAGAACAAAGTCATGAGGC+TGG | + | chr8.4:23835989-23836008 | None:intergenic | 45.0% | |
| TGTAGGAATATGGGGATGCA+AGG | - | chr8.4:23835873-23835892 | MS.gene46387:intron | 45.0% | |
| ! | ATGATGGAAAAGGGGAGAAG+GGG | + | chr8.4:23834778-23834797 | None:intergenic | 45.0% |
| ! | TTTTTCATAAGCCAGGCCCT+AGG | - | chr8.4:23835435-23835454 | MS.gene46387:intron | 45.0% |
| !! | GATGATGGAAAAGGGGAGAA+GGG | + | chr8.4:23834779-23834798 | None:intergenic | 45.0% |
| AAAAGGGGAGAAGGGGAGAA+GGG | + | chr8.4:23834771-23834790 | None:intergenic | 50.0% | |
| GCTGTGAAGAGAAAGGCTGT+AGG | - | chr8.4:23835856-23835875 | MS.gene46387:CDS | 50.0% | |
| TGCGGTAAAGTGAAAGCAGG+CGG | - | chr8.4:23835898-23835917 | MS.gene46387:intron | 50.0% | |
| TTGTTCCCTCAACCTCCTGA+TGG | + | chr8.4:23836053-23836072 | None:intergenic | 50.0% | |
| !! | GGATGATGGAAAAGGGGAGA+AGG | + | chr8.4:23834780-23834799 | None:intergenic | 50.0% |
| AAAGGGGAGAAGGGGAGAAG+GGG | + | chr8.4:23834770-23834789 | None:intergenic | 55.0% | |
| GAAAAGGGGAGAAGGGGAGA+AGG | + | chr8.4:23834772-23834791 | None:intergenic | 55.0% | |
| GAGCACCATCAGGAGGTTGA+GGG | - | chr8.4:23836045-23836064 | MS.gene46387:intron | 55.0% | |
| GATGGTGCTCCGAACAGTCA+CGG | + | chr8.4:23836035-23836054 | None:intergenic | 55.0% | |
| ! | CTGCAAGTGCCGTGACTGTT+CGG | - | chr8.4:23836023-23836042 | MS.gene46387:intron | 55.0% |
| ! | TGACTGTTCGGAGCACCATC+AGG | - | chr8.4:23836035-23836054 | MS.gene46387:intron | 55.0% |
| ! | CTGTTCGGAGCACCATCAGG+AGG | - | chr8.4:23836038-23836057 | MS.gene46387:intron | 60.0% |
| ! | GGAGCACCATCAGGAGGTTG+AGG | - | chr8.4:23836044-23836063 | MS.gene46387:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 23834753 | 23836084 | 23834753 | ID=MS.gene46387 |
| chr8.4 | mRNA | 23834753 | 23836084 | 23834753 | ID=MS.gene46387.t1;Parent=MS.gene46387 |
| chr8.4 | exon | 23836082 | 23836084 | 23836082 | ID=MS.gene46387.t1.exon1;Parent=MS.gene46387.t1 |
| chr8.4 | CDS | 23836082 | 23836084 | 23836082 | ID=cds.MS.gene46387.t1;Parent=MS.gene46387.t1 |
| chr8.4 | exon | 23835848 | 23835887 | 23835848 | ID=MS.gene46387.t1.exon2;Parent=MS.gene46387.t1 |
| chr8.4 | CDS | 23835848 | 23835887 | 23835848 | ID=cds.MS.gene46387.t1;Parent=MS.gene46387.t1 |
| chr8.4 | exon | 23835099 | 23835187 | 23835099 | ID=MS.gene46387.t1.exon3;Parent=MS.gene46387.t1 |
| chr8.4 | CDS | 23835099 | 23835187 | 23835099 | ID=cds.MS.gene46387.t1;Parent=MS.gene46387.t1 |
| chr8.4 | exon | 23834902 | 23834984 | 23834902 | ID=MS.gene46387.t1.exon4;Parent=MS.gene46387.t1 |
| chr8.4 | CDS | 23834902 | 23834984 | 23834902 | ID=cds.MS.gene46387.t1;Parent=MS.gene46387.t1 |
| chr8.4 | exon | 23834753 | 23834816 | 23834753 | ID=MS.gene46387.t1.exon5;Parent=MS.gene46387.t1 |
| chr8.4 | CDS | 23834753 | 23834816 | 23834753 | ID=cds.MS.gene46387.t1;Parent=MS.gene46387.t1 |
| Gene Sequence |
| Protein sequence |
