Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47344.t1 | XP_014512522.1 | 96.1 | 76 | 3 | 0 | 1 | 76 | 9 | 84 | 2.80E-34 | 154.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47344.t1 | O81864 | 83.6 | 73 | 12 | 0 | 4 | 76 | 115 | 187 | 4.7e-30 | 131.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47344.t1 | A0A1S3V2W2 | 96.1 | 76 | 3 | 0 | 1 | 76 | 9 | 84 | 2.0e-34 | 154.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene47344 | 0.823191 | 1.58E-53 | -1.69E-46 |
| MS.gene049171 | MS.gene47344 | 0.810564 | 1.08E-50 | -1.69E-46 |
| MS.gene049257 | MS.gene47344 | 0.831707 | 1.43E-55 | -1.69E-46 |
| MS.gene050315 | MS.gene47344 | 0.812553 | 4.00E-51 | -1.69E-46 |
| MS.gene05081 | MS.gene47344 | 0.865632 | 5.13E-65 | -1.69E-46 |
| MS.gene051171 | MS.gene47344 | 0.815137 | 1.08E-51 | -1.69E-46 |
| MS.gene051244 | MS.gene47344 | 0.827034 | 1.95E-54 | -1.69E-46 |
| MS.gene051525 | MS.gene47344 | 0.816265 | 6.05E-52 | -1.69E-46 |
| MS.gene051552 | MS.gene47344 | -0.817517 | 3.16E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene47344 | 0.854996 | 8.48E-62 | -1.69E-46 |
| MS.gene052134 | MS.gene47344 | 0.847515 | 1.10E-59 | -1.69E-46 |
| MS.gene052135 | MS.gene47344 | 0.813608 | 2.35E-51 | -1.69E-46 |
| MS.gene052414 | MS.gene47344 | 0.801743 | 7.80E-49 | -1.69E-46 |
| MS.gene052469 | MS.gene47344 | 0.808454 | 3.07E-50 | -1.69E-46 |
| MS.gene052481 | MS.gene47344 | 0.800666 | 1.29E-48 | -1.69E-46 |
| MS.gene052501 | MS.gene47344 | 0.825353 | 4.91E-54 | -1.69E-46 |
| MS.gene052503 | MS.gene47344 | 0.803992 | 2.67E-49 | -1.69E-46 |
| MS.gene052505 | MS.gene47344 | 0.822547 | 2.23E-53 | -1.69E-46 |
| MS.gene052589 | MS.gene47344 | 0.813165 | 2.94E-51 | -1.69E-46 |
| MS.gene052825 | MS.gene47344 | 0.803811 | 2.92E-49 | -1.69E-46 |
| MS.gene052982 | MS.gene47344 | 0.803596 | 3.23E-49 | -1.69E-46 |
| MS.gene053330 | MS.gene47344 | 0.846602 | 1.96E-59 | -1.69E-46 |
| MS.gene054343 | MS.gene47344 | 0.803257 | 3.80E-49 | -1.69E-46 |
| MS.gene054344 | MS.gene47344 | 0.808461 | 3.06E-50 | -1.69E-46 |
| MS.gene054884 | MS.gene47344 | -0.822058 | 2.90E-53 | -1.69E-46 |
| MS.gene055206 | MS.gene47344 | 0.827689 | 1.36E-54 | -1.69E-46 |
| MS.gene055370 | MS.gene47344 | 0.869227 | 3.63E-66 | -1.69E-46 |
| MS.gene055539 | MS.gene47344 | 0.807599 | 4.67E-50 | -1.69E-46 |
| MS.gene056351 | MS.gene47344 | 0.862291 | 5.63E-64 | -1.69E-46 |
| MS.gene056633 | MS.gene47344 | 0.860549 | 1.91E-63 | -1.69E-46 |
| MS.gene056664 | MS.gene47344 | 0.823947 | 1.05E-53 | -1.69E-46 |
| MS.gene056796 | MS.gene47344 | 0.836224 | 1.06E-56 | -1.69E-46 |
| MS.gene056877 | MS.gene47344 | 0.838301 | 3.12E-57 | -1.69E-46 |
| MS.gene057048 | MS.gene47344 | 0.835075 | 2.07E-56 | -1.69E-46 |
| MS.gene057196 | MS.gene47344 | 0.859804 | 3.21E-63 | -1.69E-46 |
| MS.gene057332 | MS.gene47344 | 0.851826 | 6.89E-61 | -1.69E-46 |
| MS.gene057658 | MS.gene47344 | 0.865982 | 3.98E-65 | -1.69E-46 |
| MS.gene058418 | MS.gene47344 | -0.813758 | 2.18E-51 | -1.69E-46 |
| MS.gene058444 | MS.gene47344 | 0.835833 | 1.33E-56 | -1.69E-46 |
| MS.gene058746 | MS.gene47344 | 0.861749 | 8.25E-64 | -1.69E-46 |
| MS.gene058838 | MS.gene47344 | 0.847011 | 1.51E-59 | -1.69E-46 |
| MS.gene059023 | MS.gene47344 | 0.838185 | 3.34E-57 | -1.69E-46 |
| MS.gene05914 | MS.gene47344 | 0.848003 | 8.08E-60 | -1.69E-46 |
| MS.gene059303 | MS.gene47344 | 0.815798 | 7.69E-52 | -1.69E-46 |
| MS.gene059512 | MS.gene47344 | 0.886109 | 4.55E-72 | -1.69E-46 |
| MS.gene06092 | MS.gene47344 | 0.815159 | 1.07E-51 | -1.69E-46 |
| MS.gene061231 | MS.gene47344 | 0.842876 | 1.98E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47344.t1 | MTR_1g061080 | 98.630 | 73 | 1 | 0 | 4 | 76 | 106 | 178 | 9.68e-49 | 153 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47344.t1 | AT4G19830 | 83.562 | 73 | 12 | 0 | 4 | 76 | 115 | 187 | 9.94e-40 | 130 |
Find 26 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTCTTTGTCCTTGGTTC+TGG | 0.255718 | 1.1:-46417391 | MS.gene47344:CDS |
| ATTCTCATCTTTGTGGTCAT+AGG | 0.366810 | 1.1:+46417429 | None:intergenic |
| TGCTGTGTTCAGGTAATTGC+TGG | 0.390936 | 1.1:-46416905 | MS.gene47344:intron |
| CTTTGTCCTTGGTTCTGGTA+AGG | 0.410071 | 1.1:-46417386 | MS.gene47344:intron |
| GATCACCATTCTCATCTTTG+TGG | 0.422175 | 1.1:+46417422 | None:intergenic |
| TTGTTTGTTTACTAGATGGC+TGG | 0.432490 | 1.1:+46416426 | None:intergenic |
| GTGTTTGTTTGTTTACTAGA+TGG | 0.436943 | 1.1:+46416422 | None:intergenic |
| AGGACAAAGACAAAAGGATT+AGG | 0.454496 | 1.1:+46417400 | None:intergenic |
| TTTCTTACATTAGGCGGGAT+AGG | 0.475564 | 1.1:+46416791 | None:intergenic |
| GTCATCATACCTCCCTCACT+TGG | 0.492147 | 1.1:-46416836 | MS.gene47344:CDS |
| AAGGATTTCTTACATTAGGC+GGG | 0.525661 | 1.1:+46416786 | None:intergenic |
| AAAGGATTTCTTACATTAGG+CGG | 0.534022 | 1.1:+46416785 | None:intergenic |
| GAACCAAGGACAAAGACAAA+AGG | 0.544303 | 1.1:+46417394 | None:intergenic |
| TATGACCACAAAGATGAGAA+TGG | 0.579877 | 1.1:-46417427 | MS.gene47344:CDS |
| GGTAGACTTGCAGCAAAACA+AGG | 0.581972 | 1.1:-46417469 | MS.gene47344:intron |
| GACTTGCAGCAAAACAAGGA+TGG | 0.607232 | 1.1:-46417465 | MS.gene47344:CDS |
| TTCTGATACCCAAGTGAGGG+AGG | 0.616633 | 1.1:+46416827 | None:intergenic |
| TCATCATACCTCCCTCACTT+GGG | 0.617977 | 1.1:-46416835 | MS.gene47344:CDS |
| TGGGTATCAGAACACATCAC+AGG | 0.647520 | 1.1:-46416816 | MS.gene47344:CDS |
| AGTTCCTCAAGATGGAGACC+AGG | 0.651081 | 1.1:-46417793 | None:intergenic |
| GTGAGATCAATGAAAGTCGG+TGG | 0.665800 | 1.1:-46416869 | MS.gene47344:CDS |
| AGCTTACCTTACCAGAACCA+AGG | 0.667313 | 1.1:+46417380 | None:intergenic |
| GGTAATTGCTGGAATTGATG+TGG | 0.679818 | 1.1:-46416894 | MS.gene47344:CDS |
| GTGTTCTGATACCCAAGTGA+GGG | 0.691969 | 1.1:+46416824 | None:intergenic |
| GCAGTGAGATCAATGAAAGT+CGG | 0.693946 | 1.1:-46416872 | MS.gene47344:CDS |
| TGTGTTCTGATACCCAAGTG+AGG | 0.735364 | 1.1:+46416823 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGGTTTTTTTTATTTATTTA+GGG | - | chr1.1:46416602-46416621 | MS.gene47344:intron | 10.0% |
| !!! | TAGGTTTTTTTTATTTATTT+AGG | - | chr1.1:46416601-46416620 | MS.gene47344:intron | 10.0% |
| !!! | TTTGTTTTATTTTATTTTGT+TGG | - | chr1.1:46416561-46416580 | MS.gene47344:intron | 10.0% |
| !! | AAAAATTTCAGACTTATATA+TGG | - | chr1.1:46417205-46417224 | MS.gene47344:intron | 15.0% |
| !! | AAATTCAGAATTTATAGATA+TGG | - | chr1.1:46416521-46416540 | MS.gene47344:intron | 15.0% |
| !! | AGTAAATGTTATTATATTGA+AGG | - | chr1.1:46416710-46416729 | MS.gene47344:intron | 15.0% |
| !! | AAATTCTGAATTTAGCATAT+TGG | + | chr1.1:46416514-46416533 | None:intergenic | 20.0% |
| !! | AATGCTAATAGACAAATTAA+TGG | - | chr1.1:46416626-46416645 | MS.gene47344:intron | 20.0% |
| !! | ACTTATATTTGATATTGAAC+TGG | - | chr1.1:46417738-46417757 | MS.gene47344:intron | 20.0% |
| !! | ATAAAAGGATTTCTTACATT+AGG | + | chr1.1:46417440-46417459 | None:intergenic | 20.0% |
| !! | ATATATATATAGGAGATTTG+AGG | + | chr1.1:46416484-46416503 | None:intergenic | 20.0% |
| !! | TTGATTATAACCAGTAAATT+TGG | - | chr1.1:46416861-46416880 | MS.gene47344:CDS | 20.0% |
| !!! | ACAAACTTATTTTGATCATT+TGG | + | chr1.1:46417029-46417048 | None:intergenic | 20.0% |
| !!! | TAATAGACAAATTAATGGAT+AGG | - | chr1.1:46416631-46416650 | MS.gene47344:intron | 20.0% |
| !!! | TGGTTTCTAGATATATATAT+AGG | + | chr1.1:46416494-46416513 | None:intergenic | 20.0% |
| ! | AAACAAAAAAGTTCTTTGAC+AGG | - | chr1.1:46417647-46417666 | MS.gene47344:intron | 25.0% |
| ! | ATATTTGATATTGAACTGGT+TGG | - | chr1.1:46417742-46417761 | MS.gene47344:intron | 25.0% |
| ! | ATGTAATTCTGGATCATAAA+AGG | + | chr1.1:46417455-46417474 | None:intergenic | 25.0% |
| ! | TATAACCAGTAAATTTGGTT+TGG | - | chr1.1:46416866-46416885 | MS.gene47344:CDS | 25.0% |
| !! | TGAAATTTTTACTGTCCTTA+TGG | + | chr1.1:46417195-46417214 | None:intergenic | 25.0% |
| !!! | CATACTAAGTTTTTTCCATA+AGG | - | chr1.1:46417177-46417196 | MS.gene47344:intron | 25.0% |
| AAACACCAAACCAAATTTAC+TGG | + | chr1.1:46416874-46416893 | None:intergenic | 30.0% | |
| AAAGGATTTCTTACATTAGG+CGG | + | chr1.1:46417437-46417456 | None:intergenic | 30.0% | |
| AAGGTTGAGATTCATTACTA+TGG | - | chr1.1:46416729-46416748 | MS.gene47344:intron | 30.0% | |
| AGAAGTTAATCTTGTGAGAA+TGG | - | chr1.1:46417289-46417308 | MS.gene47344:intron | 30.0% | |
| CAAACGTAACTATGTAATTC+TGG | + | chr1.1:46417466-46417485 | None:intergenic | 30.0% | |
| CAGACTTATATATGGATCTT+TGG | - | chr1.1:46417213-46417232 | MS.gene47344:intron | 30.0% | |
| CTTAGTATGCAACATGATAA+AGG | + | chr1.1:46417267-46417286 | None:intergenic | 30.0% | |
| ! | AGCAATTTTAAGCATCAATG+TGG | - | chr1.1:46417121-46417140 | MS.gene47344:intron | 30.0% |
| ! | GACAAATTAATGGATAGGTA+AGG | - | chr1.1:46416636-46416655 | MS.gene47344:intron | 30.0% |
| ! | TAAAATTGCTTCTTGAGTTG+TGG | + | chr1.1:46417111-46417130 | None:intergenic | 30.0% |
| AAGGATTTCTTACATTAGGC+GGG | + | chr1.1:46417436-46417455 | None:intergenic | 35.0% | |
| AAGTCGAGTCGGATTAAAAA+TGG | + | chr1.1:46417689-46417708 | None:intergenic | 35.0% | |
| AGGACAAAGACAAAAGGATT+AGG | + | chr1.1:46416822-46416841 | None:intergenic | 35.0% | |
| ATTCTCATCTTTGTGGTCAT+AGG | + | chr1.1:46416793-46416812 | None:intergenic | 35.0% | |
| GGTAGAGTATGAGAATTTGT+AGG | - | chr1.1:46416582-46416601 | MS.gene47344:intron | 35.0% | |
| TATGACCACAAAGATGAGAA+TGG | - | chr1.1:46416792-46416811 | MS.gene47344:intron | 35.0% | |
| TTAAATGAGAGAGCTATACC+TGG | + | chr1.1:46416447-46416466 | None:intergenic | 35.0% | |
| ! | AATCCTTTTGTCTTTGTCCT+TGG | - | chr1.1:46416822-46416841 | MS.gene47344:CDS | 35.0% |
| ! | ATGCAACATGATAAAGGCAT+AGG | + | chr1.1:46417261-46417280 | None:intergenic | 35.0% |
| AAAAGTTCTTTGACAGGCAG+AGG | - | chr1.1:46417653-46417672 | MS.gene47344:intron | 40.0% | |
| AATGGTGAAGGTTCGACTTT+AGG | - | chr1.1:46417712-46417731 | MS.gene47344:intron | 40.0% | |
| ATGGTGAAGGTTCGACTTTA+GGG | - | chr1.1:46417713-46417732 | MS.gene47344:intron | 40.0% | |
| GAACCAAGGACAAAGACAAA+AGG | + | chr1.1:46416828-46416847 | None:intergenic | 40.0% | |
| GATCACCATTCTCATCTTTG+TGG | + | chr1.1:46416800-46416819 | None:intergenic | 40.0% | |
| GCAGTGAGATCAATGAAAGT+CGG | - | chr1.1:46417347-46417366 | MS.gene47344:intron | 40.0% | |
| GGTAATTGCTGGAATTGATG+TGG | - | chr1.1:46417325-46417344 | MS.gene47344:intron | 40.0% | |
| TTTCTTACATTAGGCGGGAT+AGG | + | chr1.1:46417431-46417450 | None:intergenic | 40.0% | |
| TTTGTCTTTGTCCTTGGTTC+TGG | - | chr1.1:46416828-46416847 | MS.gene47344:CDS | 40.0% | |
| !! | ATATTGAACTGGTTGGTCTG+AGG | - | chr1.1:46417749-46417768 | MS.gene47344:intron | 40.0% |
| AGCTTACCTTACCAGAACCA+AGG | + | chr1.1:46416842-46416861 | None:intergenic | 45.0% | |
| GACTTGCAGCAAAACAAGGA+TGG | - | chr1.1:46416754-46416773 | MS.gene47344:intron | 45.0% | |
| GGTAGACTTGCAGCAAAACA+AGG | - | chr1.1:46416750-46416769 | MS.gene47344:intron | 45.0% | |
| GTGAGATCAATGAAAGTCGG+TGG | - | chr1.1:46417350-46417369 | MS.gene47344:intron | 45.0% | |
| GTGTTCTGATACCCAAGTGA+GGG | + | chr1.1:46417398-46417417 | None:intergenic | 45.0% | |
| TCACCATTTGCAAGTCGAGT+CGG | + | chr1.1:46417700-46417719 | None:intergenic | 45.0% | |
| TCATCATACCTCCCTCACTT+GGG | - | chr1.1:46417384-46417403 | MS.gene47344:intron | 45.0% | |
| TGCTGTGTTCAGGTAATTGC+TGG | - | chr1.1:46417314-46417333 | MS.gene47344:intron | 45.0% | |
| TGGGTATCAGAACACATCAC+AGG | - | chr1.1:46417403-46417422 | MS.gene47344:CDS | 45.0% | |
| TGTGTTCTGATACCCAAGTG+AGG | + | chr1.1:46417399-46417418 | None:intergenic | 45.0% | |
| ! | AATCCGACTCGACTTGCAAA+TGG | - | chr1.1:46417694-46417713 | MS.gene47344:intron | 45.0% |
| ! | ACTCGACTTGCAAATGGTGA+AGG | - | chr1.1:46417700-46417719 | MS.gene47344:intron | 45.0% |
| !! | CTTTGTCCTTGGTTCTGGTA+AGG | - | chr1.1:46416833-46416852 | MS.gene47344:CDS | 45.0% |
| !! | GAGAATGGATTGCTGTGTTC+AGG | - | chr1.1:46417304-46417323 | MS.gene47344:intron | 45.0% |
| GTCATCATACCTCCCTCACT+TGG | - | chr1.1:46417383-46417402 | MS.gene47344:intron | 50.0% | |
| TTCTGATACCCAAGTGAGGG+AGG | + | chr1.1:46417395-46417414 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 46416437 | 46417804 | 46416437 | ID=MS.gene47344 |
| chr1.1 | mRNA | 46416437 | 46417804 | 46416437 | ID=MS.gene47344.t1;Parent=MS.gene47344 |
| chr1.1 | exon | 46417794 | 46417804 | 46417794 | ID=MS.gene47344.t1.exon1;Parent=MS.gene47344.t1 |
| chr1.1 | CDS | 46417794 | 46417804 | 46417794 | ID=cds.MS.gene47344.t1;Parent=MS.gene47344.t1 |
| chr1.1 | exon | 46417387 | 46417486 | 46417387 | ID=MS.gene47344.t1.exon2;Parent=MS.gene47344.t1 |
| chr1.1 | CDS | 46417387 | 46417486 | 46417387 | ID=cds.MS.gene47344.t1;Parent=MS.gene47344.t1 |
| chr1.1 | exon | 46416799 | 46416915 | 46416799 | ID=MS.gene47344.t1.exon3;Parent=MS.gene47344.t1 |
| chr1.1 | CDS | 46416799 | 46416915 | 46416799 | ID=cds.MS.gene47344.t1;Parent=MS.gene47344.t1 |
| chr1.1 | exon | 46416437 | 46416454 | 46416437 | ID=MS.gene47344.t1.exon4;Parent=MS.gene47344.t1 |
| chr1.1 | CDS | 46416437 | 46416454 | 46416437 | ID=cds.MS.gene47344.t1;Parent=MS.gene47344.t1 |
| Gene Sequence |
| Protein sequence |
