AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47903


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47903.t1 MTR_2g088700 92.982 57 4 0 93 149 88 144 4.71e-30 111
MS.gene47903.t1 MTR_2g088700 92.982 57 4 0 93 149 88 144 4.75e-30 111
MS.gene47903.t1 MTR_6g028140 48.148 54 28 0 93 146 83 136 5.89e-11 59.7
MS.gene47903.t1 MTR_5g089200 44.872 78 42 1 13 90 151 227 8.53e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47903.t1 AT3G03800 53.571 56 26 0 93 148 84 139 3.39e-14 68.9
MS.gene47903.t1 AT3G03800 53.571 56 26 0 93 148 84 139 3.39e-14 68.9
MS.gene47903.t1 AT5G08080 57.447 47 20 0 93 139 83 129 7.86e-13 64.3
MS.gene47903.t1 AT5G08080 57.447 47 20 0 93 139 83 129 7.86e-13 64.3
MS.gene47903.t1 AT5G08080 57.447 47 20 0 93 139 83 129 9.73e-13 64.7
MS.gene47903.t1 AT5G08080 57.447 47 20 0 93 139 83 129 9.73e-13 64.7
MS.gene47903.t1 AT5G08080 57.447 47 20 0 93 139 94 140 9.92e-13 64.7

Find 23 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGGATCTTGACAGAGTTCC+AGG 0.316919 4.1:+11721943 MS.gene47903:CDS
TTAAGAAGCGCAAGTCTCTT+TGG 0.358710 4.1:+11721061 MS.gene47903:CDS
CGTCTATGTTTCTTAATCTC+AGG 0.392267 4.1:+11720964 MS.gene47903:CDS
TTACATAGAACATACCTTAT+GGG 0.397491 4.1:-11720439 None:intergenic
CATTCTTGCTCCGTCAATAC+CGG 0.466523 4.1:-11721805 None:intergenic
TTATGAAGAGGATTTGAAGC+AGG 0.468923 4.1:+11720800 MS.gene47903:CDS
TATGATAATTTCATCCCATA+AGG 0.480276 4.1:+11720425 None:intergenic
GAAAAGGATATTGATGAGGT+TGG 0.486934 4.1:+11721592 MS.gene47903:intron
CAAAAGCCTGGCTGTGAGAA+GGG 0.499127 4.1:+11721780 MS.gene47903:CDS
CCGGTTCCCTTCTCACAGCC+AGG 0.501105 4.1:-11721786 None:intergenic
CCTGGCTGTGAGAAGGGAAC+CGG 0.506291 4.1:+11721786 MS.gene47903:CDS
GAGAAGGGAACCGGTATTGA+CGG 0.524006 4.1:+11721795 MS.gene47903:CDS
CTTTGGTTCATGCAGCTGCT+CGG 0.524841 4.1:+11720603 MS.gene47903:CDS
TTGGTGCCTTAGCGATGGTA+AGG 0.529609 4.1:+11721080 MS.gene47903:CDS
CTAAACAATAGACAAAAGCC+TGG 0.535553 4.1:+11721768 MS.gene47903:CDS
ACAAAAGCCTGGCTGTGAGA+AGG 0.542434 4.1:+11721779 MS.gene47903:CDS
CAACATTGAAGATTATGAAG+AGG 0.549840 4.1:+11720788 MS.gene47903:CDS
TCTCTTTGGTGCCTTAGCGA+TGG 0.574751 4.1:+11721075 MS.gene47903:CDS
AAAATACCTTACCATCGCTA+AGG 0.592387 4.1:-11721086 None:intergenic
AGAGCTGCAATTAAGTTCAG+AGG 0.598621 4.1:+11720744 MS.gene47903:CDS
AATTAAGTTCAGAGGAGTCG+AGG 0.627825 4.1:+11720752 MS.gene47903:CDS
GATGGAAAAGGATATTGATG+AGG 0.655016 4.1:+11721588 MS.gene47903:intron
GGTTGGAAAGATTGCACATG+TGG 0.657598 4.1:+11721609 MS.gene47903:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAGCATATAATAAATTTT+TGG + chr4.1:11720695-11720714 MS.gene47903:intron 10.0%
!! AAAAATATTCTCAACATTAA+AGG - chr4.1:11720565-11720584 None:intergenic 15.0%
!! AAATTGAATGTAAAAATTGT+AGG + chr4.1:11720472-11720491 MS.gene47903:intron 15.0%
!! AATAATTGATTGAAAACTAT+AGG - chr4.1:11720877-11720896 None:intergenic 15.0%
!! AATTGAATGTAAAAATTGTA+GGG + chr4.1:11720473-11720492 MS.gene47903:intron 15.0%
!! TGTTATATAGATTTCAAATA+TGG + chr4.1:11721707-11721726 MS.gene47903:intron 15.0%
!!! AAATTTCATTCATTCTTTTA+TGG + chr4.1:11720653-11720672 MS.gene47903:intron 15.0%
!!! ATCAATTATTACCTTTTTTT+TGG + chr4.1:11720887-11720906 MS.gene47903:intron 15.0%
!!! TAATGTTGAGAATATTTTTT+CGG + chr4.1:11720566-11720585 MS.gene47903:intron 15.0%
!!! TTACATTGATTGATTTTATT+GGG + chr4.1:11722464-11722483 MS.gene47903:intron 15.0%
!!! TTTACATTGATTGATTTTAT+TGG + chr4.1:11722463-11722482 MS.gene47903:intron 15.0%
!! AAGTTTACATTTCAAAATAC+AGG - chr4.1:11722301-11722320 None:intergenic 20.0%
!! AATTCAAGATACCAAAAAAA+AGG - chr4.1:11720901-11720920 None:intergenic 20.0%
!! ATGGATAAAAAATAGTTAAC+CGG + chr4.1:11721726-11721745 MS.gene47903:intron 20.0%
!!! AATGTTGAGAATATTTTTTC+GGG + chr4.1:11720567-11720586 MS.gene47903:intron 20.0%
!!! AATTTTAACTTCAATGTGAA+TGG + chr4.1:11721285-11721304 MS.gene47903:intron 20.0%
!!! ACTTTGTATATCATGAATTT+TGG - chr4.1:11722197-11722216 None:intergenic 20.0%
!!! TGTAATTGTTTTATGATGAA+TGG + chr4.1:11720511-11720530 MS.gene47903:intron 20.0%
! AACACAGTTAACTTTATCAA+GGG - chr4.1:11722125-11722144 None:intergenic 25.0%
! TAAAATTCATGATATGCACA+AGG + chr4.1:11722246-11722265 MS.gene47903:intron 25.0%
! TGGATAAAAAATAGTTAACC+GGG + chr4.1:11721727-11721746 MS.gene47903:intron 25.0%
! TGTTGTGATTTAAATGAGTT+TGG + chr4.1:11720920-11720939 MS.gene47903:intron 25.0%
! TTACATAGAACATACCTTAT+GGG - chr4.1:11720442-11720461 None:intergenic 25.0%
! TTTACATAGAACATACCTTA+TGG - chr4.1:11720443-11720462 None:intergenic 25.0%
!! AAGGTTTGAACATGTAATTT+TGG + chr4.1:11722425-11722444 MS.gene47903:intron 25.0%
!! TAATGCTCATAAGATTTTAC+TGG + chr4.1:11721534-11721553 MS.gene47903:intron 25.0%
!! TCTGAAAATCAATTTGTTTC+AGG + chr4.1:11720534-11720553 MS.gene47903:intron 25.0%
!! TGAAATGTAAACTTTGAGAA+AGG + chr4.1:11722307-11722326 MS.gene47903:intron 25.0%
!!! ATGTTGAGAATATTTTTTCG+GGG + chr4.1:11720568-11720587 MS.gene47903:intron 25.0%
!!! GAAAAACAACTTCTAGTTTT+AGG - chr4.1:11721986-11722005 None:intergenic 25.0%
AATACAAGATGAATATCGTG+AGG + chr4.1:11722059-11722078 MS.gene47903:intron 30.0%
ACACAGTTAACTTTATCAAG+GGG - chr4.1:11722124-11722143 None:intergenic 30.0%
AGTTCCTTGACTAAAAAGTT+CGG + chr4.1:11721922-11721941 MS.gene47903:intron 30.0%
AGTTTGGAGGAATTAATTTC+AGG + chr4.1:11720936-11720955 MS.gene47903:intron 30.0%
ATCATAACAATTGTCTTGGA+TGG + chr4.1:11721570-11721589 MS.gene47903:intron 30.0%
ATCTACTTTGATATGACTCA+TGG + chr4.1:11722379-11722398 MS.gene47903:intron 30.0%
ATTACATGTTCAAACCTTTG+TGG - chr4.1:11722423-11722442 None:intergenic 30.0%
ATTCTGCATAAAACATATCC+CGG - chr4.1:11721748-11721767 None:intergenic 30.0%
CAACACAGTTAACTTTATCA+AGG - chr4.1:11722126-11722145 None:intergenic 30.0%
CAACATTGAAGATTATGAAG+AGG + chr4.1:11720788-11720807 MS.gene47903:CDS 30.0%
CATTTCAAAATACAGGCAAA+TGG - chr4.1:11722294-11722313 None:intergenic 30.0%
CTGAATCATAACAATTGTCT+TGG + chr4.1:11721566-11721585 MS.gene47903:intron 30.0%
TATTCTCAACATTAAAGGAC+AGG - chr4.1:11720560-11720579 None:intergenic 30.0%
TGAGAAGATAGACATAAACT+TGG - chr4.1:11722008-11722027 None:intergenic 30.0%
TGCACTTAGTAACAACAAAA+GGG - chr4.1:11722501-11722520 None:intergenic 30.0%
TGTGATTTAAATGAGTTTGG+AGG + chr4.1:11720923-11720942 MS.gene47903:intron 30.0%
! AAGTTTTTCATGGCATACAA+AGG + chr4.1:11721000-11721019 MS.gene47903:CDS 30.0%
! AATGAAAGTTGAGATCCTTT+TGG + chr4.1:11722332-11722351 MS.gene47903:intron 30.0%
! GTAGTACTTTAGAAATTTGC+TGG + chr4.1:11722153-11722172 MS.gene47903:intron 30.0%
! TCATAAGATTTTACTGGTAC+TGG + chr4.1:11721540-11721559 MS.gene47903:intron 30.0%
!! TAGTTCTGATTATTGTGATC+TGG + chr4.1:11721165-11721184 MS.gene47903:intron 30.0%
!!! CAACTCTTTCAAGTTTTTCA+TGG + chr4.1:11720990-11721009 MS.gene47903:CDS 30.0%
!!! GGCATACAAAGGATTTTTTA+TGG + chr4.1:11721011-11721030 MS.gene47903:CDS 30.0%
!!! TGTTGAGAATATTTTTTCGG+GGG + chr4.1:11720569-11720588 MS.gene47903:intron 30.0%
AAAATACCTTACCATCGCTA+AGG - chr4.1:11721089-11721108 None:intergenic 35.0%
AAGTTAACTGTGTTGCACTA+CGG + chr4.1:11722131-11722150 MS.gene47903:intron 35.0%
ACAATTGTCTTGGATGGAAA+AGG + chr4.1:11721576-11721595 MS.gene47903:intron 35.0%
CCTTGTAACAATACCTTTCA+TGG - chr4.1:11721475-11721494 None:intergenic 35.0%
CGCATGGAGTATAAGAAAAT+TGG + chr4.1:11721383-11721402 MS.gene47903:intron 35.0%
CGTCTATGTTTCTTAATCTC+AGG + chr4.1:11720964-11720983 MS.gene47903:CDS 35.0%
CTAAACAATAGACAAAAGCC+TGG + chr4.1:11721768-11721787 MS.gene47903:CDS 35.0%
CTGCACTTAGTAACAACAAA+AGG - chr4.1:11722502-11722521 None:intergenic 35.0%
GAAAAGGATATTGATGAGGT+TGG + chr4.1:11721592-11721611 MS.gene47903:intron 35.0%
GATGGAAAAGGATATTGATG+AGG + chr4.1:11721588-11721607 MS.gene47903:intron 35.0%
GTTCCTTGACTAAAAAGTTC+GGG + chr4.1:11721923-11721942 MS.gene47903:intron 35.0%
TTCCTTGACTAAAAAGTTCG+GGG + chr4.1:11721924-11721943 MS.gene47903:CDS 35.0%
! ATAGCAATAGCAATTTTCGC+AGG + chr4.1:11721410-11721429 MS.gene47903:intron 35.0%
! ATTTGAAGCAGGTGAGTTAT+GGG + chr4.1:11720811-11720830 MS.gene47903:intron 35.0%
! CAAGTTTCAGTATTTTCGCA+TGG + chr4.1:11721367-11721386 MS.gene47903:intron 35.0%
! CATGGAATGAGATAGCTTTT+TGG + chr4.1:11722397-11722416 MS.gene47903:intron 35.0%
! GGCAATGTCTGTAATTCTTT+TGG + chr4.1:11721493-11721512 MS.gene47903:intron 35.0%
! TTATGAAGAGGATTTGAAGC+AGG + chr4.1:11720800-11720819 MS.gene47903:CDS 35.0%
!! ACAAAAGCATTTGCCATGAA+AGG + chr4.1:11721459-11721478 MS.gene47903:intron 35.0%
!! CCATGAAAGGTATTGTTACA+AGG + chr4.1:11721472-11721491 MS.gene47903:intron 35.0%
!!! CTTTTGTTGTTACTAAGTGC+AGG + chr4.1:11722500-11722519 MS.gene47903:intron 35.0%
AATTAAGTTCAGAGGAGTCG+AGG + chr4.1:11720752-11720771 MS.gene47903:CDS 40.0%
AGAGCTGCAATTAAGTTCAG+AGG + chr4.1:11720744-11720763 MS.gene47903:CDS 40.0%
AGATGAATATCGTGAGGTTG+TGG + chr4.1:11722065-11722084 MS.gene47903:intron 40.0%
GCAGAATAAGTGTGACCAAA+AGG - chr4.1:11722350-11722369 None:intergenic 40.0%
GTCAAGGAACTGCAATTTCA+AGG - chr4.1:11721913-11721932 None:intergenic 40.0%
GTGGAGAGAAGAGTTATTAC+AGG + chr4.1:11722084-11722103 MS.gene47903:intron 40.0%
GTGTTTCAGAACAACATACC+TGG - chr4.1:11721964-11721983 None:intergenic 40.0%
! ATTTTCGCAGGAAGCTAATG+AGG + chr4.1:11721422-11721441 MS.gene47903:intron 40.0%
! GATTTGAAGCAGGTGAGTTA+TGG + chr4.1:11720810-11720829 MS.gene47903:intron 40.0%
! TTAAGAAGCGCAAGTCTCTT+TGG + chr4.1:11721061-11721080 MS.gene47903:CDS 40.0%
! TTTGAAGCAGGTGAGTTATG+GGG + chr4.1:11720812-11720831 MS.gene47903:intron 40.0%
!! AGATAGCTTTTTGGCCACAA+AGG + chr4.1:11722406-11722425 MS.gene47903:intron 40.0%
!! ATCCCCGAACTTTTTAGTCA+AGG - chr4.1:11721929-11721948 None:intergenic 40.0%
CATTCTTGCTCCGTCAATAC+CGG - chr4.1:11721808-11721827 None:intergenic 45.0%
GGTTGGAAAGATTGCACATG+TGG + chr4.1:11721609-11721628 MS.gene47903:CDS 45.0%
ACAAAAGCCTGGCTGTGAGA+AGG + chr4.1:11721779-11721798 MS.gene47903:CDS 50.0%
CAAAAGCCTGGCTGTGAGAA+GGG + chr4.1:11721780-11721799 MS.gene47903:CDS 50.0%
CGGGGGTTATCAGTACTCTT+TGG + chr4.1:11720586-11720605 MS.gene47903:intron 50.0%
! CTTTGGTTCATGCAGCTGCT+CGG + chr4.1:11720603-11720622 MS.gene47903:CDS 50.0%
! GAGAAGGGAACCGGTATTGA+CGG + chr4.1:11721795-11721814 MS.gene47903:CDS 50.0%
! TTGGTGCCTTAGCGATGGTA+AGG + chr4.1:11721080-11721099 MS.gene47903:CDS 50.0%
!! TCTCTTTGGTGCCTTAGCGA+TGG + chr4.1:11721075-11721094 MS.gene47903:CDS 50.0%
GGGGATCTTGACAGAGTTCC+AGG + chr4.1:11721943-11721962 MS.gene47903:CDS 55.0%
CCTGGCTGTGAGAAGGGAAC+CGG + chr4.1:11721786-11721805 MS.gene47903:CDS 60.0%
CCGGTTCCCTTCTCACAGCC+AGG - chr4.1:11721789-11721808 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 11720426 11722553 11720426 ID=MS.gene47903
chr4.1 mRNA 11720426 11722553 11720426 ID=MS.gene47903.t1;Parent=MS.gene47903
chr4.1 exon 11720426 11720446 11720426 ID=MS.gene47903.t1.exon1;Parent=MS.gene47903.t1
chr4.1 CDS 11720426 11720446 11720426 ID=cds.MS.gene47903.t1;Parent=MS.gene47903.t1
chr4.1 exon 11720599 11720624 11720599 ID=MS.gene47903.t1.exon2;Parent=MS.gene47903.t1
chr4.1 CDS 11720599 11720624 11720599 ID=cds.MS.gene47903.t1;Parent=MS.gene47903.t1
chr4.1 exon 11720734 11720821 11720734 ID=MS.gene47903.t1.exon3;Parent=MS.gene47903.t1
chr4.1 CDS 11720734 11720821 11720734 ID=cds.MS.gene47903.t1;Parent=MS.gene47903.t1
chr4.1 exon 11720958 11721101 11720958 ID=MS.gene47903.t1.exon4;Parent=MS.gene47903.t1
chr4.1 CDS 11720958 11721101 11720958 ID=cds.MS.gene47903.t1;Parent=MS.gene47903.t1
chr4.1 exon 11721598 11721663 11721598 ID=MS.gene47903.t1.exon5;Parent=MS.gene47903.t1
chr4.1 CDS 11721598 11721663 11721598 ID=cds.MS.gene47903.t1;Parent=MS.gene47903.t1
chr4.1 exon 11721765 11721838 11721765 ID=MS.gene47903.t1.exon6;Parent=MS.gene47903.t1
chr4.1 CDS 11721765 11721838 11721765 ID=cds.MS.gene47903.t1;Parent=MS.gene47903.t1
chr4.1 exon 11721924 11721964 11721924 ID=MS.gene47903.t1.exon7;Parent=MS.gene47903.t1
chr4.1 CDS 11721924 11721964 11721924 ID=cds.MS.gene47903.t1;Parent=MS.gene47903.t1
chr4.1 exon 11722522 11722553 11722522 ID=MS.gene47903.t1.exon8;Parent=MS.gene47903.t1
chr4.1 CDS 11722522 11722553 11722522 ID=cds.MS.gene47903.t1;Parent=MS.gene47903.t1
Gene Sequence

>MS.gene47903

ATGATAATTTCATCCCATAAGGTATGTTCTATGTAAATTTATATTTAAATTGAATGTAAAAATTGTAGGGTTAAAATATTGATGTTGTAATTGTTTTATGATGAATGGTCTGAAAATCAATTTGTTTCAGGCCTGTCCTTTAATGTTGAGAATATTTTTTCGGGGGTTATCAGTACTCTTTGGTTCATGCAGCTGCTCGGTGAGTCATCATTAATTTAATTCATATAAAATTTCATTCATTCTTTTATGGTTAAAAATACATACATATATTTAGCATATAATAAATTTTTGGTGTTTTTTCTACTCAGTGCATATGATAGAGCTGCAATTAAGTTCAGAGGAGTCGAGGCAGATATAAATTTCAACATTGAAGATTATGAAGAGGATTTGAAGCAGGTGAGTTATGGGGAAGAATATGTTAAAAAAATGTTTATACTTTATATAATATCCTATAGTTTTCAATCAATTATTACCTTTTTTTTGGTATCTTGAATTGTTGTGATTTAAATGAGTTTGGAGGAATTAATTTCAGGTAGTTCGTCTATGTTTCTTAATCTCAGGTATCAACTCTTTCAAGTTTTTCATGGCATACAAAGGATTTTTTATGGTCAACGATGAGCTTCTTCTGCAAGTATTTAAGAAGCGCAAGTCTCTTTGGTGCCTTAGCGATGGTAAGGTATTTTTAGTTAAGCTTTTCATTTGAAACTGAATGATTGTTTGAGTTGATTTGTATAGTTCGTAGTTCTGATTATTGTGATCTGGACTGTATTGAAAGATATTTTAATTTGTTGATTAAGATGATGTGAGTTGTGTAACTAAATCTGATGCATTATGATCATATTTTTTAGAAAAATCAATCAATTTTAACTTCAATGTGAATGGCAGTTAATTATGAAGAGACAAAAATAGTGTATGCATGTCAAATTTTGTGATGTTCAACTCAAGTTTCAGTATTTTCGCATGGAGTATAAGAAAATTGGATGTATAGCAATAGCAATTTTCGCAGGAAGCTAATGAGGAATCAAATGCTGTTACAAAAGCATTTGCCATGAAAGGTATTGTTACAAGGCAATGTCTGTAATTCTTTTGGTTGATACTTCAAATTGTTTAATGCTCATAAGATTTTACTGGTACTGGTTACTGAATCATAACAATTGTCTTGGATGGAAAAGGATATTGATGAGGTTGGAAAGATTGCACATGTGGTCAAAGCAAAAATTGAAGCTATCAACAGAAAAGTAATGTGATGCTAGCTGAATATTGTTTTAGCATATTTCAGTTTGTTATATAGATTTCAAATATGGATAAAAAATAGTTAACCGGGATATGTTTTATGCAGAATCTAAACAATAGACAAAAGCCTGGCTGTGAGAAGGGAACCGGTATTGACGGAGCAAGAATGAATATGACAAAGTAAATATATAGTGTATGTGTTTATTAGCATACTTGATAAAAACAATTTAAGAAGCTAAATATCTACTTTTCCTTGAAATTGCAGTTCCTTGACTAAAAAGTTCGGGGATCTTGACAGAGTTCCAGGTATGTTGTTCTGAAACACCTAAAACTAGAAGTTGTTTTTCCAAGTTTATGTCTATCTTCTCATCAACGTGTATGTTATGTTGCAGAGACAAAGAATACAAGATGAATATCGTGAGGTTGTGGAGAGAAGAGTTATTACAGGTATGAGCATTTTATCCCCTTGATAAAGTTAACTGTGTTGCACTACGGTAGTACTTTAGAAATTTGCTGGCTTTCTCATGTGATGTCACCAAAATTCATGATATACAAAGTTTTTTGATCATACGAAATTTCATTATATATAAAATTCATGATATGCACAAGGTATAGAAATTGCTAATTGATTGCCATTTGCCTGTATTTTGAAATGTAAACTTTGAGAAAGGAGAATGAAAGTTGAGATCCTTTTGGTCACACTTATTCTGCAGCAAGTTCATCTACTTTGATATGACTCATGGAATGAGATAGCTTTTTGGCCACAAAGGTTTGAACATGTAATTTTGGTCTTAGATTTATTTCTTTACATTGATTGATTTTATTGGGACATTTACAATCCCTTTTGTTGTTACTAAGTGCAGGTGAAGAACAAAGATTTGAAATTGAAAGTTAA

Protein sequence

>MS.gene47903.t1

MIISSHKYSLVHAAARAYDRAAIKFRGVEADINFNIEDYEEDLKQVVRLCFLISGINSFKFFMAYKGFFMVNDELLLQVFKKRKSLWCLSDGKDIDEVGKIAHVVKAKIEAINRKNLNNRQKPGCEKGTGIDGARMNMTNSLTKKFGDLDRVPGEEQRFEIES