AlfalfaGEDB | MS.gene48054

M. sativa cultivar XinJiangDaYe / MS.gene48054

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48054.t1	XP_024640147.1	95.4	196	9	0	1	196	1	196	7.20E-100	373.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48054.t1	Q9LK65	54.4	114	50	1	85	196	76	189	4.5e-26	119.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48054.t1	G7K4G4	95.4	196	9	0	1	196	1	196	5.2e-100	373.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04915	MS.gene48054	0.844968	5.44E-59	-1.69E-46
MS.gene049178	MS.gene48054	0.805021	1.63E-49	-1.69E-46
MS.gene04917	MS.gene48054	0.843274	1.55E-58	-1.69E-46
MS.gene049378	MS.gene48054	0.842969	1.87E-58	-1.69E-46
MS.gene049382	MS.gene48054	0.81456	1.45E-51	-1.69E-46
MS.gene04984	MS.gene48054	0.872405	3.26E-67	-1.69E-46
MS.gene04991	MS.gene48054	-0.808388	3.17E-50	-1.69E-46
MS.gene050114	MS.gene48054	0.824682	7.07E-54	-1.69E-46
MS.gene050226	MS.gene48054	0.840302	9.44E-58	-1.69E-46
MS.gene050227	MS.gene48054	0.841658	4.16E-58	-1.69E-46
MS.gene050228	MS.gene48054	0.835922	1.27E-56	-1.69E-46
MS.gene050623	MS.gene48054	-0.835714	1.43E-56	-1.69E-46
MS.gene050629	MS.gene48054	-0.830584	2.71E-55	-1.69E-46
MS.gene050762	MS.gene48054	0.879851	8.89E-70	-1.69E-46
MS.gene050763	MS.gene48054	0.880575	4.91E-70	-1.69E-46
MS.gene051210	MS.gene48054	0.813331	2.70E-51	-1.69E-46
MS.gene051663	MS.gene48054	0.800743	1.25E-48	-1.69E-46
MS.gene051790	MS.gene48054	0.804296	2.31E-49	-1.69E-46
MS.gene052856	MS.gene48054	0.834556	2.80E-56	-1.69E-46
MS.gene052858	MS.gene48054	0.817263	3.61E-52	-1.69E-46
MS.gene052860	MS.gene48054	0.811439	7.00E-51	-1.69E-46
MS.gene054379	MS.gene48054	0.875822	2.28E-68	-1.69E-46
MS.gene054930	MS.gene48054	0.826317	2.90E-54	-1.69E-46
MS.gene056400	MS.gene48054	0.806076	9.80E-50	-1.69E-46
MS.gene056552	MS.gene48054	0.810922	9.06E-51	-1.69E-46
MS.gene057665	MS.gene48054	0.830039	3.68E-55	-1.69E-46
MS.gene057929	MS.gene48054	0.818406	1.99E-52	-1.69E-46
MS.gene058638	MS.gene48054	0.801626	8.24E-49	-1.69E-46
MS.gene058639	MS.gene48054	0.804921	1.71E-49	-1.69E-46
MS.gene058758	MS.gene48054	0.820251	7.57E-53	-1.69E-46
MS.gene059713	MS.gene48054	0.834264	3.31E-56	-1.69E-46
MS.gene059719	MS.gene48054	0.836609	8.47E-57	-1.69E-46
MS.gene059725	MS.gene48054	0.812041	5.18E-51	-1.69E-46
MS.gene060084	MS.gene48054	0.82277	1.98E-53	-1.69E-46
MS.gene060380	MS.gene48054	0.803142	4.01E-49	-1.69E-46
MS.gene060637	MS.gene48054	0.819131	1.36E-52	-1.69E-46
MS.gene060683	MS.gene48054	0.806584	7.66E-50	-1.69E-46
MS.gene060685	MS.gene48054	0.800347	1.50E-48	-1.69E-46
MS.gene060869	MS.gene48054	0.832607	8.58E-56	-1.69E-46
MS.gene060870	MS.gene48054	0.813923	2.00E-51	-1.69E-46
MS.gene060998	MS.gene48054	0.807152	5.81E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48054.t1	MTR_5g094740	95.408	196	9	0	1	196	1	196	2.56e-122	365

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48054.t1	AT1G55930	63.333	90	30	1	107	196	98	184	2.43e-33	125
MS.gene48054.t1	AT1G55930	63.333	90	30	1	107	196	98	184	3.03e-33	125
MS.gene48054.t1	AT3G13070	54.386	114	50	1	85	196	76	189	5.89e-27	107

Find 41 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAAATTCTTCGTTTGTTTA+AGG	0.144682	5.3:-76914208	MS.gene48054:CDS
ATTGTTATTGGCTTTGCTTT+TGG	0.245514	5.3:-76914178	MS.gene48054:CDS
CCTTCAGTAGCCAAAACTTT+AGG	0.245516	5.3:+76914303	None:intergenic
GAGAATGCCAATGAAGGTTT+TGG	0.249627	5.3:-76914598	MS.gene48054:CDS
GGTGAAGAGAGGAATTATTT+TGG	0.252414	5.3:-76914361	MS.gene48054:CDS
GACACTCGAATCAGGGGTTC+TGG	0.292964	5.3:-76914682	MS.gene48054:CDS
TAAGGAACAAGGATTGTTAT+TGG	0.294468	5.3:-76914190	MS.gene48054:CDS
CTTCAGTAGCCAAAACTTTA+GGG	0.296810	5.3:+76914304	None:intergenic
GTTTGTGGGGTGTTGGTGTT+TGG	0.313415	5.3:-76914330	MS.gene48054:CDS
AATGCTGGATATGGTGTTAT+TGG	0.317649	5.3:-76914273	MS.gene48054:CDS
AGCTTTCATCTTTCAAGTTA+TGG	0.321638	5.3:-76914513	MS.gene48054:CDS
GTTTGGATGCCCTAAAGTTT+TGG	0.332563	5.3:-76914313	MS.gene48054:CDS
GAAGTTTACAAATGAAGTTT+TGG	0.355145	5.3:-76914382	MS.gene48054:CDS
TTTGTACCCAAAACCTTCAT+TGG	0.380761	5.3:+76914591	None:intergenic
TTAGCAATGACACTCGAATC+AGG	0.381702	5.3:-76914690	None:intergenic
TGCTACTGTTTGTGGGGTGT+TGG	0.407645	5.3:-76914337	MS.gene48054:CDS
GGTGTGGTGAATGCTGGATA+TGG	0.447491	5.3:-76914282	MS.gene48054:CDS
ACTGAAGGTGTGGTGAATGC+TGG	0.457072	5.3:-76914288	MS.gene48054:CDS
CAGTTGGACACAATAGGAAA+AGG	0.462993	5.3:+76914558	None:intergenic
ACACTCGAATCAGGGGTTCT+GGG	0.470559	5.3:-76914681	MS.gene48054:CDS
CTTCGTTTGTTTAAGGAACA+AGG	0.470581	5.3:-76914201	MS.gene48054:CDS
AGCTTGAAGGTGAAGTGTAT+TGG	0.479042	5.3:-76914462	MS.gene48054:CDS
TTTGTACGATTCTCATGCAC+AGG	0.480881	5.3:+76914621	None:intergenic
CCAACACAGTTGGACACAAT+AGG	0.482123	5.3:+76914552	None:intergenic
CGATTACTACACTTTGGCCT+TGG	0.498418	5.3:-76914122	MS.gene48054:CDS
CCTATTGTGTCCAACTGTGT+TGG	0.498989	5.3:-76914552	MS.gene48054:CDS
AGACTTCGATTACTACACTT+TGG	0.504973	5.3:-76914128	MS.gene48054:CDS
TACTACACTTTGGCCTTGGA+AGG	0.509755	5.3:-76914118	None:intergenic
TGTAAACTTCAAATTAGCAT+CGG	0.521085	5.3:+76914395	None:intergenic
TACAAAGAGAATGCCAATGA+AGG	0.521281	5.3:-76914604	MS.gene48054:CDS
AGTTGGTGGAGCTAGCTTGA+AGG	0.527234	5.3:-76914475	MS.gene48054:CDS
AGTTGGACACAATAGGAAAA+GGG	0.534542	5.3:+76914559	None:intergenic
TAGCAATGACACTCGAATCA+GGG	0.545433	5.3:-76914689	None:intergenic
AATAAGCATGATACCTTCCA+AGG	0.560169	5.3:+76914105	None:intergenic
ATTGAAAGGGCCAACACAGT+TGG	0.567652	5.3:+76914542	None:intergenic
TTTGGCTGCTACTGTTTGTG+GGG	0.569636	5.3:-76914343	MS.gene48054:CDS
AAGGTGAAGTGTATTGGTAG+TGG	0.586954	5.3:-76914456	MS.gene48054:CDS
AGCAATGACACTCGAATCAG+GGG	0.613707	5.3:-76914688	None:intergenic
GCTTAAGAGTACTTGGCCGA+CGG	0.617799	5.3:-76914235	MS.gene48054:CDS
CGAAGAATTTGCAACACCGT+CGG	0.668231	5.3:+76914219	None:intergenic
TTGTACGATTCTCATGCACA+GGG	0.692351	5.3:+76914622	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAATTTGAATTTAGTTGG+TGG	-	chr5.3:76914314-76914333	MS.gene48054:CDS	20.0%
!!	GGAAAAAATTTGAATTTAGT+TGG	-	chr5.3:76914311-76914330	MS.gene48054:CDS	20.0%
!	TGTAAACTTCAAATTAGCAT+CGG	+	chr5.3:76914411-76914430	None:intergenic	25.0%
!!	GAAAGCTTTTACAATTGAAA+GGG	+	chr5.3:76914277-76914296	None:intergenic	25.0%
!!	GAAGTTTACAAATGAAGTTT+TGG	-	chr5.3:76914421-76914440	MS.gene48054:CDS	25.0%
!!	TGAAAGCTTTTACAATTGAA+AGG	+	chr5.3:76914278-76914297	None:intergenic	25.0%
	GCAAATTCTTCGTTTGTTTA+AGG	-	chr5.3:76914595-76914614	MS.gene48054:CDS	30.0%
	TAAGGAACAAGGATTGTTAT+TGG	-	chr5.3:76914613-76914632	MS.gene48054:CDS	30.0%
!!	AGCTTTCATCTTTCAAGTTA+TGG	-	chr5.3:76914290-76914309	MS.gene48054:CDS	30.0%
!!!	ATTGTTATTGGCTTTGCTTT+TGG	-	chr5.3:76914625-76914644	MS.gene48054:CDS	30.0%
!!!	TACTTTTGCTTAAGAGTACT+TGG	-	chr5.3:76914561-76914580	MS.gene48054:CDS	30.0%
!!!	TTGTTATTGGCTTTGCTTTT+GGG	-	chr5.3:76914626-76914645	MS.gene48054:CDS	30.0%
	AGACTTCGATTACTACACTT+TGG	-	chr5.3:76914675-76914694	MS.gene48054:CDS	35.0%
	AGTTGGACACAATAGGAAAA+GGG	+	chr5.3:76914247-76914266	None:intergenic	35.0%
	CTTCAGTAGCCAAAACTTTA+GGG	+	chr5.3:76914502-76914521	None:intergenic	35.0%
	CTTCGTTTGTTTAAGGAACA+AGG	-	chr5.3:76914602-76914621	MS.gene48054:CDS	35.0%
	TACAAAGAGAATGCCAATGA+AGG	-	chr5.3:76914199-76914218	MS.gene48054:CDS	35.0%
	TTTGTACCCAAAACCTTCAT+TGG	+	chr5.3:76914215-76914234	None:intergenic	35.0%
!	AGAATGCCAATGAAGGTTTT+GGG	-	chr5.3:76914206-76914225	MS.gene48054:CDS	35.0%
!	GGTGAAGAGAGGAATTATTT+TGG	-	chr5.3:76914442-76914461	MS.gene48054:CDS	35.0%
!!	AATGAAGTTTTGGTGAAGAG+AGG	-	chr5.3:76914431-76914450	MS.gene48054:CDS	35.0%
!!	AATGCTGGATATGGTGTTAT+TGG	-	chr5.3:76914530-76914549	MS.gene48054:CDS	35.0%
!!!	ATGAAGGTTTTGGGTACAAA+TGG	-	chr5.3:76914215-76914234	MS.gene48054:CDS	35.0%
!!!	TGAAGGTTTTGGGTACAAAT+GGG	-	chr5.3:76914216-76914235	MS.gene48054:CDS	35.0%
	AGCTTGAAGGTGAAGTGTAT+TGG	-	chr5.3:76914341-76914360	MS.gene48054:CDS	40.0%
	CAGTTGGACACAATAGGAAA+AGG	+	chr5.3:76914248-76914267	None:intergenic	40.0%
	CCTTCAGTAGCCAAAACTTT+AGG	+	chr5.3:76914503-76914522	None:intergenic	40.0%
	TTGTACGATTCTCATGCACA+GGG	+	chr5.3:76914184-76914203	None:intergenic	40.0%
	TTTGTACGATTCTCATGCAC+AGG	+	chr5.3:76914185-76914204	None:intergenic	40.0%
!	AAGGTGAAGTGTATTGGTAG+TGG	-	chr5.3:76914347-76914366	MS.gene48054:CDS	40.0%
!	GAGAATGCCAATGAAGGTTT+TGG	-	chr5.3:76914205-76914224	MS.gene48054:CDS	40.0%
!	GGGTCATCGAGTTTTCATTT+CGG	-	chr5.3:76914142-76914161	MS.gene48054:CDS	40.0%
!	GGTCATCGAGTTTTCATTTC+GGG	-	chr5.3:76914143-76914162	MS.gene48054:CDS	40.0%
!	GTTTGGATGCCCTAAAGTTT+TGG	-	chr5.3:76914490-76914509	MS.gene48054:CDS	40.0%
!!	TTTTGGCTGCTACTGTTTGT+GGG	-	chr5.3:76914459-76914478	MS.gene48054:CDS	40.0%
!!!	ATTTTGGCTGCTACTGTTTG+TGG	-	chr5.3:76914458-76914477	MS.gene48054:CDS	40.0%
!!!	CCTAAAGTTTTGGCTACTGA+AGG	-	chr5.3:76914500-76914519	MS.gene48054:CDS	40.0%
!!!	TCTTTCGGCTTTTTTCTCTC+TGG	-	chr5.3:76914649-76914668	MS.gene48054:CDS	40.0%
	ATTGAAAGGGCCAACACAGT+TGG	+	chr5.3:76914264-76914283	None:intergenic	45.0%
	CCAACACAGTTGGACACAAT+AGG	+	chr5.3:76914254-76914273	None:intergenic	45.0%
	CCTATTGTGTCCAACTGTGT+TGG	-	chr5.3:76914251-76914270	MS.gene48054:CDS	45.0%
	CGAAGAATTTGCAACACCGT+CGG	+	chr5.3:76914587-76914606	None:intergenic	45.0%
	CGATTACTACACTTTGGCCT+TGG	-	chr5.3:76914681-76914700	MS.gene48054:CDS	45.0%
	TTTGGCTGCTACTGTTTGTG+GGG	-	chr5.3:76914460-76914479	MS.gene48054:CDS	45.0%
!!	GGCTTTGCTTTTGGGTCTTT+CGG	-	chr5.3:76914634-76914653	MS.gene48054:CDS	45.0%
!!!	AGTTTTGGCTACTGAAGGTG+TGG	-	chr5.3:76914505-76914524	MS.gene48054:CDS	45.0%
	ACACTCGAATCAGGGGTTCT+GGG	-	chr5.3:76914122-76914141	MS.gene48054:CDS	50.0%
	GGTGTGGTGAATGCTGGATA+TGG	-	chr5.3:76914521-76914540	MS.gene48054:CDS	50.0%
	TGCTACTGTTTGTGGGGTGT+TGG	-	chr5.3:76914466-76914485	MS.gene48054:CDS	50.0%
!	ACTGAAGGTGTGGTGAATGC+TGG	-	chr5.3:76914515-76914534	MS.gene48054:CDS	50.0%
!!	AGTTGGTGGAGCTAGCTTGA+AGG	-	chr5.3:76914328-76914347	MS.gene48054:CDS	50.0%
!!	GCTTAAGAGTACTTGGCCGA+CGG	-	chr5.3:76914568-76914587	MS.gene48054:CDS	50.0%
!!	GTTTGTGGGGTGTTGGTGTT+TGG	-	chr5.3:76914473-76914492	MS.gene48054:CDS	50.0%
	GACACTCGAATCAGGGGTTC+TGG	-	chr5.3:76914121-76914140	MS.gene48054:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	76914119	76914706	76914119	ID=MS.gene48054
chr5.3	mRNA	76914119	76914706	76914119	ID=MS.gene48054.t1;Parent=MS.gene48054
chr5.3	exon	76914119	76914706	76914119	ID=MS.gene48054.t1.exon1;Parent=MS.gene48054.t1
chr5.3	CDS	76914119	76914706	76914119	ID=cds.MS.gene48054.t1;Parent=MS.gene48054.t1

Gene Sequence

>MS.gene48054

ATGACACTCGAATCAGGGGTTCTGGGTCATCGAGTTTTCATTTCGGGTTCGAAATTGTTTTACCCTGTGCATGAGAATCGTACAAAGAGAATGCCAATGAAGGTTTTGGGTACAAATGGGAGATACCCTTTTCCTATTGTGTCCAACTGTGTTGGCCCTTTCAATTGTAAAAGCTTTCATCTTTCAAGTTATGGAAAAAATTTGAATTTAGTTGGTGGAGCTAGCTTGAAGGTGAAGTGTATTGGTAGTGGTAGTGATAGTGATTGTTTGCGAGCTAAAAACAATGTTTCCGATGCTAATTTGAAGTTTACAAATGAAGTTTTGGTGAAGAGAGGAATTATTTTGGCTGCTACTGTTTGTGGGGTGTTGGTGTTTGGATGCCCTAAAGTTTTGGCTACTGAAGGTGTGGTGAATGCTGGATATGGTGTTATTGGAAAGTGTATACTTTTGCTTAAGAGTACTTGGCCGACGGTGTTGCAAATTCTTCGTTTGTTTAAGGAACAAGGATTGTTATTGGCTTTGCTTTTGGGTCTTTCGGCTTTTTTCTCTCTGGCAGAGACTTCGATTACTACACTTTGGCCTTGGAAG

Protein sequence

>MS.gene48054.t1

MTLESGVLGHRVFISGSKLFYPVHENRTKRMPMKVLGTNGRYPFPIVSNCVGPFNCKSFHLSSYGKNLNLVGGASLKVKCIGSGSDSDCLRAKNNVSDANLKFTNEVLVKRGIILAATVCGVLVFGCPKVLATEGVVNAGYGVIGKCILLLKSTWPTVLQILRLFKEQGLLLALLLGLSAFFSLAETSITTLWPWK