AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48059


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48059.t1 MTR_5g091310 95.652 69 3 0 1 69 18 86 1.87e-44 137
MS.gene48059.t1 MTR_4g094635 50.685 73 29 3 1 69 21 90 3.30e-16 66.6
MS.gene48059.t1 MTR_4g094630 50.000 72 28 3 3 69 6 74 2.10e-15 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48059.t1 AT5G46295 60.465 43 16 1 27 69 30 71 2.84e-13 58.5
MS.gene48059.t1 AT1G06475 55.556 45 19 1 26 69 49 93 4.37e-12 56.2

Find 10 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAGTGAAGAAAGAAATT+TGG 0.267366 5.1:+72763285 MS.gene48059:CDS
CATGGAGATTCTCTGCGTTT+AGG 0.350104 5.1:+72763309 MS.gene48059:CDS
TCGTGGATACTGTTGTGTTT+AGG 0.353121 5.1:+72763363 MS.gene48059:CDS
TGCTTCTTCTATCTTTGTTG+TGG 0.457389 5.1:+72763428 MS.gene48059:CDS
GAAAGAAGAGTAGCATGTTA+TGG 0.462290 5.1:+72763258 MS.gene48059:CDS
TCATCTTCAAATGACATTCG+TGG 0.492578 5.1:+72763346 MS.gene48059:CDS
GGAGATTCTCTGCGTTTAGG+TGG 0.556332 5.1:+72763312 MS.gene48059:CDS
TGAAGAAAGAAATTTGGCCA+TGG 0.584343 5.1:+72763291 MS.gene48059:CDS
AAGATAGAAGAAGCAAACAG+TGG 0.638854 5.1:-72763420 None:intergenic
CTAAACGCAGAGAATCTCCA+TGG 0.659319 5.1:-72763308 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAAGTGAAGAAAGAAATT+TGG + chr5.1:72763285-72763304 MS.gene48059:CDS 25.0%
AAGATAGAAGAAGCAAACAG+TGG - chr5.1:72763423-72763442 None:intergenic 35.0%
GAAAGAAGAGTAGCATGTTA+TGG + chr5.1:72763258-72763277 MS.gene48059:CDS 35.0%
TCATCTTCAAATGACATTCG+TGG + chr5.1:72763346-72763365 MS.gene48059:CDS 35.0%
TGAAGAAAGAAATTTGGCCA+TGG + chr5.1:72763291-72763310 MS.gene48059:CDS 35.0%
TGCTTCTTCTATCTTTGTTG+TGG + chr5.1:72763428-72763447 MS.gene48059:CDS 35.0%
TCGTGGATACTGTTGTGTTT+AGG + chr5.1:72763363-72763382 MS.gene48059:CDS 40.0%
CATGGAGATTCTCTGCGTTT+AGG + chr5.1:72763309-72763328 MS.gene48059:CDS 45.0%
CTAAACGCAGAGAATCTCCA+TGG - chr5.1:72763311-72763330 None:intergenic 45.0%
GGAGATTCTCTGCGTTTAGG+TGG + chr5.1:72763312-72763331 MS.gene48059:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 72763254 72763463 72763254 ID=MS.gene48059
chr5.1 mRNA 72763254 72763463 72763254 ID=MS.gene48059.t1;Parent=MS.gene48059
chr5.1 exon 72763254 72763463 72763254 ID=MS.gene48059.t1.exon1;Parent=MS.gene48059.t1
chr5.1 CDS 72763254 72763463 72763254 ID=cds.MS.gene48059.t1;Parent=MS.gene48059.t1
Gene Sequence

>MS.gene48059

ATGGGAAAGAAGAGTAGCATGTTATGGTTGAAGAAAGTGAAGAAAGAAATTTGGCCATGGAGATTCTCTGCGTTTAGGTGGAAACGACTTGATCATCTTCAAATGACATTCGTGGATACTGTTGTGTTTAGGATACTCTCTGTTGCTGAAGCTGTTGTTCTTGTCTCCACTGTTTGCTTCTTCTATCTTTGTTGTGGATGCAATTTCTGA

Protein sequence

>MS.gene48059.t1

MGKKSSMLWLKKVKKEIWPWRFSAFRWKRLDHLQMTFVDTVVFRILSVAEAVVLVSTVCFFYLCCGCNF