Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48733.t1 | XP_003605420.2 | 96.8 | 309 | 10 | 0 | 1 | 309 | 12 | 320 | 2.80E-167 | 597.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48733.t1 | Q9SJZ2 | 71.8 | 309 | 87 | 0 | 1 | 309 | 7 | 315 | 1.2e-129 | 464.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48733.t1 | G7JKV1 | 96.8 | 309 | 10 | 0 | 1 | 309 | 12 | 320 | 2.0e-167 | 597.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene48733 | MS.gene029047 | PPI |
| MS.gene48733 | MS.gene57204 | PPI |
| MS.gene48733 | MS.gene20276 | PPI |
| MS.gene48733 | MS.gene09575 | PPI |
| MS.gene48733 | MS.gene072888 | PPI |
| MS.gene48733 | MS.gene35795 | PPI |
| MS.gene48733 | MS.gene27001 | PPI |
| MS.gene48733 | MS.gene32715 | PPI |
| MS.gene48733 | MS.gene014836 | PPI |
| MS.gene48733 | MS.gene44482 | PPI |
| MS.gene48733 | MS.gene35931 | PPI |
| MS.gene48733 | MS.gene67066 | PPI |
| MS.gene24236 | MS.gene48733 | PPI |
| MS.gene48733 | MS.gene80170 | PPI |
| MS.gene27263 | MS.gene48733 | PPI |
| MS.gene48733 | MS.gene32635 | PPI |
| MS.gene48733 | MS.gene90718 | PPI |
| MS.gene48733 | MS.gene014924 | PPI |
| MS.gene48733 | MS.gene35853 | PPI |
| MS.gene48733 | MS.gene015231 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48733.t1 | MTR_4g031140 | 96.764 | 309 | 10 | 0 | 1 | 309 | 12 | 320 | 0.0 | 617 |
| MS.gene48733.t1 | MTR_4g030890 | 85.667 | 300 | 43 | 0 | 10 | 309 | 93 | 392 | 0.0 | 550 |
| MS.gene48733.t1 | MTR_3g094650 | 46.349 | 315 | 156 | 5 | 1 | 309 | 11 | 318 | 1.32e-95 | 285 |
| MS.gene48733.t1 | MTR_8g076820 | 46.801 | 297 | 154 | 3 | 17 | 309 | 30 | 326 | 5.63e-93 | 279 |
| MS.gene48733.t1 | MTR_3g072190 | 44.937 | 316 | 159 | 5 | 2 | 309 | 6 | 314 | 2.55e-92 | 277 |
| MS.gene48733.t1 | MTR_3g466200 | 45.687 | 313 | 157 | 5 | 3 | 309 | 11 | 316 | 1.40e-91 | 275 |
| MS.gene48733.t1 | MTR_2g029800 | 45.333 | 300 | 159 | 3 | 15 | 309 | 27 | 326 | 3.03e-90 | 273 |
| MS.gene48733.t1 | MTR_5g021060 | 45.455 | 297 | 158 | 3 | 17 | 309 | 30 | 326 | 1.03e-89 | 271 |
| MS.gene48733.t1 | MTR_5g074970 | 45.597 | 318 | 153 | 6 | 2 | 309 | 15 | 322 | 3.49e-89 | 269 |
| MS.gene48733.t1 | MTR_2g029740 | 44.371 | 302 | 163 | 3 | 13 | 309 | 25 | 326 | 1.32e-88 | 269 |
| MS.gene48733.t1 | MTR_5g074860 | 44.688 | 320 | 159 | 6 | 1 | 309 | 9 | 321 | 1.17e-87 | 265 |
| MS.gene48733.t1 | MTR_2g040000 | 45.724 | 304 | 155 | 4 | 14 | 309 | 26 | 327 | 2.00e-87 | 265 |
| MS.gene48733.t1 | MTR_7g093370 | 41.905 | 315 | 177 | 4 | 1 | 309 | 53 | 367 | 3.47e-87 | 266 |
| MS.gene48733.t1 | MTR_2g029750 | 43.709 | 302 | 165 | 3 | 13 | 309 | 25 | 326 | 8.07e-87 | 264 |
| MS.gene48733.t1 | MTR_2g028980 | 43.421 | 304 | 167 | 3 | 11 | 309 | 23 | 326 | 8.14e-87 | 264 |
| MS.gene48733.t1 | MTR_5g083860 | 44.409 | 313 | 162 | 4 | 2 | 309 | 16 | 321 | 2.82e-86 | 262 |
| MS.gene48733.t1 | MTR_3g094670 | 44.872 | 312 | 160 | 4 | 3 | 309 | 14 | 318 | 1.23e-85 | 260 |
| MS.gene48733.t1 | MTR_4g122640 | 43.854 | 301 | 163 | 4 | 13 | 309 | 34 | 332 | 2.18e-85 | 260 |
| MS.gene48733.t1 | MTR_1g101830 | 44.194 | 310 | 168 | 4 | 1 | 309 | 13 | 318 | 3.51e-85 | 259 |
| MS.gene48733.t1 | MTR_1g022970 | 45.973 | 298 | 156 | 3 | 17 | 309 | 46 | 343 | 8.04e-85 | 259 |
| MS.gene48733.t1 | MTR_3g094630 | 44.762 | 315 | 160 | 5 | 2 | 309 | 11 | 318 | 1.11e-84 | 258 |
| MS.gene48733.t1 | MTR_2g029815 | 41.772 | 316 | 175 | 4 | 3 | 309 | 11 | 326 | 9.03e-84 | 256 |
| MS.gene48733.t1 | MTR_5g074740 | 43.987 | 316 | 163 | 5 | 1 | 309 | 9 | 317 | 9.73e-84 | 255 |
| MS.gene48733.t1 | MTR_5g074760 | 43.038 | 316 | 166 | 5 | 1 | 309 | 9 | 317 | 1.91e-83 | 254 |
| MS.gene48733.t1 | MTR_3g466180 | 44.373 | 311 | 163 | 3 | 2 | 309 | 16 | 319 | 5.71e-83 | 253 |
| MS.gene48733.t1 | MTR_4g083710 | 42.006 | 319 | 169 | 6 | 1 | 309 | 8 | 320 | 6.70e-83 | 253 |
| MS.gene48733.t1 | MTR_4g029170 | 43.671 | 316 | 164 | 6 | 1 | 309 | 9 | 317 | 1.44e-82 | 252 |
| MS.gene48733.t1 | MTR_7g026990 | 42.718 | 309 | 168 | 2 | 3 | 309 | 17 | 318 | 2.08e-82 | 252 |
| MS.gene48733.t1 | MTR_4g125940 | 40.881 | 318 | 176 | 5 | 2 | 309 | 10 | 325 | 6.13e-82 | 251 |
| MS.gene48733.t1 | MTR_2g084010 | 44.186 | 301 | 157 | 4 | 13 | 309 | 21 | 314 | 8.76e-82 | 250 |
| MS.gene48733.t1 | MTR_2g029830 | 42.000 | 300 | 169 | 3 | 15 | 309 | 26 | 325 | 1.18e-81 | 251 |
| MS.gene48733.t1 | MTR_2g029820 | 41.722 | 302 | 171 | 3 | 13 | 309 | 25 | 326 | 1.56e-81 | 251 |
| MS.gene48733.t1 | MTR_5g049280 | 42.056 | 321 | 173 | 6 | 1 | 309 | 14 | 333 | 2.81e-81 | 249 |
| MS.gene48733.t1 | MTR_2g029560 | 42.667 | 300 | 167 | 3 | 15 | 309 | 22 | 321 | 3.27e-81 | 249 |
| MS.gene48733.t1 | MTR_5g074710 | 43.038 | 316 | 166 | 5 | 1 | 309 | 9 | 317 | 8.21e-81 | 248 |
| MS.gene48733.t1 | MTR_2g084080 | 42.357 | 314 | 169 | 4 | 1 | 309 | 7 | 313 | 8.66e-81 | 248 |
| MS.gene48733.t1 | MTR_5g017850 | 42.357 | 314 | 174 | 5 | 1 | 309 | 11 | 322 | 1.26e-80 | 248 |
| MS.gene48733.t1 | MTR_4g029200 | 42.953 | 298 | 160 | 3 | 15 | 309 | 44 | 334 | 1.46e-80 | 248 |
| MS.gene48733.t1 | MTR_4g029180 | 44.898 | 294 | 150 | 5 | 21 | 309 | 31 | 317 | 1.48e-80 | 247 |
| MS.gene48733.t1 | MTR_1g054205 | 38.978 | 313 | 185 | 4 | 3 | 309 | 10 | 322 | 1.79e-80 | 247 |
| MS.gene48733.t1 | MTR_2g029850 | 41.722 | 302 | 171 | 3 | 13 | 309 | 25 | 326 | 7.83e-80 | 246 |
| MS.gene48733.t1 | MTR_2g029860 | 39.297 | 313 | 184 | 4 | 3 | 309 | 14 | 326 | 1.12e-79 | 246 |
| MS.gene48733.t1 | MTR_4g029190 | 42.444 | 311 | 166 | 5 | 5 | 309 | 33 | 336 | 1.28e-79 | 245 |
| MS.gene48733.t1 | MTR_2g084110 | 44.186 | 301 | 156 | 5 | 13 | 309 | 21 | 313 | 2.39e-79 | 244 |
| MS.gene48733.t1 | MTR_2g084020 | 42.857 | 301 | 161 | 4 | 13 | 309 | 21 | 314 | 2.52e-79 | 244 |
| MS.gene48733.t1 | MTR_5g014100 | 44.000 | 300 | 155 | 5 | 21 | 309 | 30 | 327 | 4.96e-79 | 243 |
| MS.gene48733.t1 | MTR_2g084090 | 42.525 | 301 | 162 | 4 | 13 | 309 | 21 | 314 | 5.16e-79 | 243 |
| MS.gene48733.t1 | MTR_2g029730 | 43.522 | 301 | 163 | 4 | 15 | 309 | 23 | 322 | 1.27e-78 | 243 |
| MS.gene48733.t1 | MTR_7g072510 | 41.026 | 312 | 174 | 3 | 1 | 309 | 4 | 308 | 4.18e-78 | 241 |
| MS.gene48733.t1 | MTR_2g029910 | 40.924 | 303 | 173 | 4 | 13 | 309 | 26 | 328 | 4.24e-78 | 242 |
| MS.gene48733.t1 | MTR_2g084000 | 40.323 | 310 | 174 | 4 | 4 | 309 | 13 | 315 | 6.43e-78 | 240 |
| MS.gene48733.t1 | MTR_1g025980 | 41.158 | 311 | 178 | 5 | 1 | 309 | 7 | 314 | 2.24e-77 | 239 |
| MS.gene48733.t1 | MTR_4g074995 | 41.270 | 315 | 156 | 7 | 5 | 309 | 9 | 304 | 2.49e-77 | 238 |
| MS.gene48733.t1 | MTR_3g467600 | 41.195 | 318 | 170 | 7 | 2 | 309 | 10 | 320 | 1.19e-76 | 237 |
| MS.gene48733.t1 | MTR_5g017860 | 40.952 | 315 | 176 | 6 | 3 | 309 | 10 | 322 | 1.84e-76 | 237 |
| MS.gene48733.t1 | MTR_4g133800 | 42.517 | 294 | 157 | 7 | 20 | 309 | 44 | 329 | 3.81e-75 | 234 |
| MS.gene48733.t1 | MTR_0155s0040 | 40.000 | 315 | 171 | 6 | 3 | 309 | 7 | 311 | 7.03e-75 | 232 |
| MS.gene48733.t1 | MTR_4g125940 | 43.173 | 271 | 145 | 4 | 46 | 309 | 2 | 270 | 1.27e-74 | 230 |
| MS.gene48733.t1 | MTR_3g072190 | 45.455 | 253 | 128 | 3 | 60 | 309 | 2 | 247 | 1.49e-74 | 229 |
| MS.gene48733.t1 | MTR_7g072480 | 41.346 | 312 | 173 | 3 | 1 | 309 | 8 | 312 | 2.41e-74 | 231 |
| MS.gene48733.t1 | MTR_4g074990 | 39.623 | 318 | 162 | 6 | 1 | 309 | 5 | 301 | 2.47e-74 | 231 |
| MS.gene48733.t1 | MTR_8g075100 | 40.667 | 300 | 173 | 4 | 11 | 309 | 20 | 315 | 6.14e-74 | 230 |
| MS.gene48733.t1 | MTR_4g046713 | 38.339 | 313 | 188 | 4 | 1 | 309 | 11 | 322 | 1.17e-73 | 229 |
| MS.gene48733.t1 | MTR_5g022870 | 40.065 | 307 | 179 | 4 | 4 | 309 | 10 | 312 | 1.99e-73 | 229 |
| MS.gene48733.t1 | MTR_1g077000 | 41.356 | 295 | 161 | 5 | 17 | 309 | 39 | 323 | 2.18e-73 | 229 |
| MS.gene48733.t1 | MTR_1g115900 | 39.557 | 316 | 175 | 6 | 2 | 309 | 10 | 317 | 4.27e-73 | 228 |
| MS.gene48733.t1 | MTR_7g029030 | 39.048 | 315 | 181 | 6 | 1 | 309 | 7 | 316 | 3.50e-72 | 226 |
| MS.gene48733.t1 | MTR_4g087965 | 38.988 | 336 | 170 | 10 | 1 | 309 | 15 | 342 | 3.67e-71 | 224 |
| MS.gene48733.t1 | MTR_1g009750 | 37.188 | 320 | 188 | 6 | 2 | 309 | 6 | 324 | 1.20e-70 | 222 |
| MS.gene48733.t1 | MTR_7g107520 | 38.079 | 302 | 180 | 5 | 11 | 309 | 19 | 316 | 6.27e-69 | 217 |
| MS.gene48733.t1 | MTR_2g088770 | 38.535 | 314 | 185 | 7 | 2 | 309 | 10 | 321 | 7.97e-69 | 217 |
| MS.gene48733.t1 | MTR_7g086820 | 38.284 | 303 | 167 | 6 | 15 | 309 | 18 | 308 | 1.01e-68 | 216 |
| MS.gene48733.t1 | MTR_2g437770 | 40.273 | 293 | 162 | 6 | 21 | 309 | 54 | 337 | 3.05e-68 | 216 |
| MS.gene48733.t1 | MTR_3g092990 | 37.793 | 299 | 182 | 3 | 15 | 309 | 24 | 322 | 1.80e-67 | 214 |
| MS.gene48733.t1 | MTR_5g016010 | 36.634 | 303 | 186 | 4 | 13 | 309 | 23 | 325 | 2.56e-67 | 213 |
| MS.gene48733.t1 | MTR_4g074980 | 41.961 | 255 | 134 | 3 | 1 | 247 | 5 | 253 | 7.82e-67 | 209 |
| MS.gene48733.t1 | MTR_7g086870 | 36.842 | 304 | 167 | 6 | 15 | 309 | 20 | 307 | 3.41e-66 | 210 |
| MS.gene48733.t1 | MTR_4g074980 | 37.143 | 315 | 163 | 6 | 1 | 309 | 5 | 290 | 5.31e-66 | 209 |
| MS.gene48733.t1 | MTR_4g114210 | 37.460 | 315 | 184 | 6 | 3 | 309 | 7 | 316 | 7.79e-66 | 209 |
| MS.gene48733.t1 | MTR_2g008710 | 38.050 | 318 | 183 | 7 | 1 | 309 | 9 | 321 | 8.71e-66 | 209 |
| MS.gene48733.t1 | MTR_1g038680 | 42.088 | 297 | 165 | 6 | 20 | 309 | 32 | 328 | 6.23e-65 | 207 |
| MS.gene48733.t1 | MTR_8g062780 | 36.508 | 315 | 193 | 4 | 1 | 309 | 7 | 320 | 3.14e-64 | 206 |
| MS.gene48733.t1 | MTR_5g074860 | 37.500 | 320 | 143 | 6 | 1 | 309 | 9 | 282 | 5.59e-64 | 203 |
| MS.gene48733.t1 | MTR_0286s0010 | 38.125 | 320 | 181 | 9 | 1 | 309 | 17 | 330 | 4.25e-63 | 202 |
| MS.gene48733.t1 | MTR_6g008075 | 38.125 | 320 | 181 | 9 | 1 | 309 | 17 | 330 | 4.25e-63 | 202 |
| MS.gene48733.t1 | MTR_1g086320 | 38.291 | 316 | 188 | 5 | 1 | 309 | 14 | 329 | 5.02e-63 | 202 |
| MS.gene48733.t1 | MTR_6g043280 | 38.567 | 293 | 167 | 7 | 21 | 309 | 45 | 328 | 8.11e-63 | 202 |
| MS.gene48733.t1 | MTR_1g090760 | 35.873 | 315 | 196 | 5 | 1 | 309 | 12 | 326 | 8.55e-62 | 199 |
| MS.gene48733.t1 | MTR_2g029830 | 40.476 | 252 | 145 | 3 | 63 | 309 | 6 | 257 | 1.05e-61 | 197 |
| MS.gene48733.t1 | MTR_6g043460 | 35.494 | 324 | 185 | 9 | 1 | 309 | 10 | 324 | 3.60e-61 | 198 |
| MS.gene48733.t1 | MTR_1g086490 | 37.771 | 323 | 187 | 5 | 1 | 309 | 14 | 336 | 3.71e-61 | 198 |
| MS.gene48733.t1 | MTR_6g027440 | 35.370 | 311 | 194 | 4 | 1 | 309 | 5 | 310 | 5.28e-61 | 197 |
| MS.gene48733.t1 | MTR_4g132490 | 34.106 | 302 | 193 | 3 | 12 | 309 | 24 | 323 | 5.80e-61 | 197 |
| MS.gene48733.t1 | MTR_5g033470 | 37.377 | 305 | 175 | 6 | 17 | 309 | 73 | 373 | 1.81e-60 | 197 |
| MS.gene48733.t1 | MTR_4g031040 | 59.770 | 174 | 27 | 2 | 76 | 249 | 1 | 131 | 1.86e-60 | 189 |
| MS.gene48733.t1 | MTR_1g009750 | 35.331 | 317 | 181 | 7 | 2 | 309 | 6 | 307 | 1.65e-59 | 193 |
| MS.gene48733.t1 | MTR_6g043240 | 37.248 | 298 | 170 | 8 | 17 | 309 | 42 | 327 | 2.63e-59 | 194 |
| MS.gene48733.t1 | MTR_2g067440 | 35.241 | 332 | 180 | 5 | 11 | 309 | 54 | 383 | 1.19e-58 | 193 |
| MS.gene48733.t1 | MTR_4g095450 | 34.202 | 307 | 191 | 5 | 9 | 309 | 19 | 320 | 1.27e-57 | 188 |
| MS.gene48733.t1 | MTR_4g127670 | 36.120 | 299 | 182 | 5 | 17 | 309 | 24 | 319 | 1.66e-57 | 188 |
| MS.gene48733.t1 | MTR_3g092990 | 35.786 | 299 | 177 | 4 | 15 | 309 | 24 | 311 | 4.14e-57 | 187 |
| MS.gene48733.t1 | MTR_2g077990 | 35.588 | 340 | 183 | 6 | 5 | 309 | 64 | 402 | 1.34e-56 | 188 |
| MS.gene48733.t1 | MTR_2g099175 | 33.887 | 301 | 186 | 5 | 17 | 309 | 36 | 331 | 2.83e-56 | 185 |
| MS.gene48733.t1 | MTR_2g078280 | 35.583 | 326 | 176 | 5 | 5 | 297 | 64 | 388 | 1.00e-55 | 185 |
| MS.gene48733.t1 | MTR_2g078610 | 35.000 | 340 | 185 | 6 | 5 | 309 | 64 | 402 | 7.37e-55 | 184 |
| MS.gene48733.t1 | MTR_1g066380 | 33.444 | 302 | 187 | 5 | 15 | 309 | 25 | 319 | 9.80e-55 | 181 |
| MS.gene48733.t1 | MTR_1g066640 | 32.450 | 302 | 190 | 5 | 15 | 309 | 25 | 319 | 1.64e-53 | 178 |
| MS.gene48733.t1 | MTR_2g008160 | 36.393 | 305 | 181 | 8 | 13 | 309 | 20 | 319 | 1.60e-52 | 175 |
| MS.gene48733.t1 | MTR_4g132110 | 32.770 | 296 | 185 | 5 | 21 | 309 | 36 | 324 | 2.18e-52 | 175 |
| MS.gene48733.t1 | MTR_1g066710 | 32.119 | 302 | 191 | 6 | 15 | 309 | 25 | 319 | 2.56e-52 | 174 |
| MS.gene48733.t1 | MTR_1g066680 | 33.113 | 302 | 187 | 6 | 15 | 309 | 25 | 318 | 7.57e-52 | 173 |
| MS.gene48733.t1 | MTR_4g132110 | 32.226 | 301 | 185 | 6 | 21 | 309 | 36 | 329 | 4.97e-50 | 169 |
| MS.gene48733.t1 | MTR_2g008730 | 34.277 | 318 | 171 | 8 | 1 | 309 | 9 | 297 | 1.53e-49 | 167 |
| MS.gene48733.t1 | MTR_3g105790 | 34.177 | 316 | 197 | 7 | 1 | 309 | 7 | 318 | 6.10e-49 | 166 |
| MS.gene48733.t1 | MTR_2g067450 | 33.981 | 309 | 187 | 6 | 11 | 309 | 5 | 306 | 7.26e-47 | 160 |
| MS.gene48733.t1 | MTR_0147s0010 | 31.329 | 316 | 180 | 7 | 17 | 309 | 77 | 378 | 3.91e-44 | 155 |
| MS.gene48733.t1 | MTR_1g066380 | 32.422 | 256 | 159 | 5 | 61 | 309 | 3 | 251 | 4.27e-41 | 144 |
| MS.gene48733.t1 | MTR_3g072210 | 42.857 | 189 | 92 | 5 | 128 | 309 | 55 | 234 | 4.58e-41 | 143 |
| MS.gene48733.t1 | MTR_2g008160 | 32.131 | 305 | 159 | 8 | 13 | 309 | 20 | 284 | 2.21e-39 | 140 |
| MS.gene48733.t1 | MTR_2g008160 | 41.379 | 174 | 98 | 3 | 13 | 186 | 20 | 189 | 3.73e-39 | 137 |
| MS.gene48733.t1 | MTR_5g074700 | 38.222 | 225 | 115 | 6 | 90 | 309 | 5 | 210 | 4.69e-38 | 134 |
| MS.gene48733.t1 | MTR_3g072260 | 53.211 | 109 | 51 | 0 | 16 | 124 | 25 | 133 | 2.17e-36 | 127 |
| MS.gene48733.t1 | MTR_7g072490 | 35.043 | 234 | 92 | 4 | 63 | 296 | 2 | 175 | 8.92e-34 | 123 |
| MS.gene48733.t1 | MTR_2g029880 | 37.433 | 187 | 100 | 4 | 129 | 309 | 9 | 184 | 2.35e-33 | 122 |
| MS.gene48733.t1 | MTR_3g105790 | 34.783 | 230 | 139 | 6 | 88 | 309 | 16 | 242 | 4.45e-31 | 117 |
| MS.gene48733.t1 | MTR_7g086790 | 33.508 | 191 | 106 | 5 | 64 | 254 | 17 | 186 | 3.07e-23 | 95.1 |
| MS.gene48733.t1 | MTR_5g058120 | 36.250 | 160 | 88 | 4 | 153 | 309 | 7 | 155 | 7.23e-23 | 95.5 |
| MS.gene48733.t1 | MTR_2g077980 | 32.234 | 273 | 140 | 11 | 79 | 309 | 11 | 280 | 1.51e-22 | 95.5 |
| MS.gene48733.t1 | MTR_2g078560 | 28.205 | 273 | 145 | 6 | 88 | 309 | 44 | 316 | 3.43e-22 | 95.1 |
| MS.gene48733.t1 | MTR_6g048160 | 54.167 | 72 | 29 | 2 | 55 | 126 | 21 | 88 | 2.06e-18 | 79.0 |
| MS.gene48733.t1 | MTR_4g114220 | 46.429 | 84 | 35 | 2 | 43 | 126 | 29 | 102 | 6.41e-17 | 76.6 |
| MS.gene48733.t1 | MTR_3g462850 | 35.507 | 138 | 79 | 5 | 181 | 309 | 9 | 145 | 1.90e-16 | 75.5 |
| MS.gene48733.t1 | MTR_7g086810 | 32.500 | 120 | 55 | 2 | 15 | 134 | 4 | 97 | 7.86e-16 | 75.9 |
| MS.gene48733.t1 | MTR_1g089005 | 31.579 | 133 | 84 | 2 | 3 | 129 | 1 | 132 | 1.10e-15 | 73.6 |
| MS.gene48733.t1 | MTR_1g098320 | 44.595 | 74 | 39 | 1 | 238 | 309 | 47 | 120 | 3.78e-13 | 66.2 |
| MS.gene48733.t1 | MTR_4g012865 | 39.726 | 73 | 35 | 1 | 17 | 80 | 77 | 149 | 1.38e-12 | 64.7 |
| MS.gene48733.t1 | MTR_2g029930 | 32.479 | 117 | 50 | 3 | 3 | 104 | 1 | 103 | 1.83e-12 | 63.2 |
| MS.gene48733.t1 | MTR_7g086785 | 29.496 | 139 | 92 | 2 | 63 | 201 | 4 | 136 | 2.92e-12 | 63.5 |
| MS.gene48733.t1 | MTR_3g107060 | 29.878 | 164 | 91 | 6 | 32 | 187 | 91 | 238 | 1.59e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48733.t1 | AT2G22420 | 71.845 | 309 | 87 | 0 | 1 | 309 | 7 | 315 | 5.69e-171 | 477 |
| MS.gene48733.t1 | AT2G18150 | 44.410 | 322 | 166 | 4 | 1 | 309 | 11 | 332 | 4.74e-95 | 285 |
| MS.gene48733.t1 | AT5G06720 | 44.654 | 318 | 164 | 4 | 4 | 309 | 12 | 329 | 1.58e-93 | 281 |
| MS.gene48733.t1 | AT4G36430 | 46.128 | 297 | 156 | 3 | 17 | 309 | 30 | 326 | 2.15e-92 | 278 |
| MS.gene48733.t1 | AT5G06730 | 43.533 | 317 | 167 | 4 | 5 | 309 | 14 | 330 | 3.51e-92 | 278 |
| MS.gene48733.t1 | AT5G19890 | 47.667 | 300 | 148 | 4 | 15 | 309 | 28 | 323 | 8.86e-92 | 276 |
| MS.gene48733.t1 | AT5G66390 | 44.781 | 297 | 160 | 3 | 17 | 309 | 33 | 329 | 1.16e-90 | 273 |
| MS.gene48733.t1 | AT2G18140 | 46.980 | 298 | 154 | 3 | 16 | 309 | 34 | 331 | 1.69e-90 | 273 |
| MS.gene48733.t1 | AT4G16270 | 47.099 | 293 | 151 | 4 | 20 | 309 | 53 | 344 | 2.33e-88 | 268 |
| MS.gene48733.t1 | AT3G50990 | 43.478 | 299 | 165 | 3 | 15 | 309 | 39 | 337 | 7.97e-87 | 264 |
| MS.gene48733.t1 | AT2G38390 | 44.224 | 303 | 163 | 4 | 13 | 309 | 27 | 329 | 7.47e-86 | 261 |
| MS.gene48733.t1 | AT5G05340 | 43.421 | 304 | 158 | 4 | 12 | 309 | 25 | 320 | 1.21e-85 | 260 |
| MS.gene48733.t1 | AT1G44970 | 45.000 | 300 | 160 | 3 | 15 | 309 | 43 | 342 | 2.56e-85 | 260 |
| MS.gene48733.t1 | AT5G19880 | 44.164 | 317 | 169 | 5 | 1 | 309 | 9 | 325 | 4.99e-85 | 259 |
| MS.gene48733.t1 | AT4G08780 | 42.671 | 307 | 169 | 4 | 10 | 309 | 17 | 323 | 3.17e-84 | 257 |
| MS.gene48733.t1 | AT2G38380 | 43.894 | 303 | 164 | 4 | 13 | 309 | 27 | 329 | 3.70e-84 | 257 |
| MS.gene48733.t1 | AT4G08770 | 42.671 | 307 | 169 | 4 | 10 | 309 | 17 | 323 | 1.40e-83 | 256 |
| MS.gene48733.t1 | AT3G32980 | 43.279 | 305 | 166 | 4 | 12 | 309 | 26 | 330 | 5.59e-83 | 254 |
| MS.gene48733.t1 | AT5G58400 | 43.492 | 315 | 165 | 5 | 1 | 309 | 14 | 321 | 2.36e-82 | 252 |
| MS.gene48733.t1 | AT1G68850 | 40.303 | 330 | 173 | 7 | 1 | 309 | 5 | 331 | 1.18e-81 | 250 |
| MS.gene48733.t1 | AT3G49110 | 42.715 | 302 | 166 | 4 | 15 | 309 | 31 | 332 | 6.91e-81 | 249 |
| MS.gene48733.t1 | AT5G51890 | 42.395 | 309 | 173 | 4 | 3 | 308 | 11 | 317 | 2.78e-80 | 246 |
| MS.gene48733.t1 | AT1G49570 | 43.046 | 302 | 166 | 4 | 14 | 309 | 45 | 346 | 1.98e-79 | 245 |
| MS.gene48733.t1 | AT3G49120 | 40.984 | 305 | 173 | 4 | 12 | 309 | 27 | 331 | 4.67e-79 | 244 |
| MS.gene48733.t1 | AT5G42180 | 41.100 | 309 | 178 | 4 | 2 | 309 | 8 | 313 | 1.18e-78 | 242 |
| MS.gene48733.t1 | AT1G14540 | 40.064 | 312 | 177 | 4 | 2 | 309 | 6 | 311 | 1.81e-78 | 241 |
| MS.gene48733.t1 | AT2G35380 | 45.033 | 302 | 157 | 5 | 17 | 309 | 30 | 331 | 6.02e-78 | 241 |
| MS.gene48733.t1 | AT5G58390 | 41.587 | 315 | 171 | 5 | 1 | 309 | 5 | 312 | 1.19e-77 | 239 |
| MS.gene48733.t1 | AT1G14550 | 40.190 | 316 | 175 | 6 | 2 | 309 | 8 | 317 | 3.99e-76 | 236 |
| MS.gene48733.t1 | AT5G17820 | 39.032 | 310 | 176 | 6 | 3 | 309 | 10 | 309 | 8.94e-76 | 234 |
| MS.gene48733.t1 | AT5G17820 | 39.032 | 310 | 176 | 6 | 3 | 309 | 63 | 362 | 8.87e-75 | 234 |
| MS.gene48733.t1 | AT3G49960 | 39.490 | 314 | 183 | 5 | 3 | 309 | 12 | 325 | 5.68e-74 | 231 |
| MS.gene48733.t1 | AT4G33420 | 42.612 | 291 | 155 | 5 | 21 | 309 | 41 | 321 | 9.77e-73 | 227 |
| MS.gene48733.t1 | AT4G33420 | 42.612 | 291 | 155 | 5 | 21 | 309 | 88 | 368 | 4.02e-72 | 227 |
| MS.gene48733.t1 | AT3G03670 | 37.580 | 314 | 184 | 4 | 3 | 309 | 9 | 317 | 3.18e-71 | 223 |
| MS.gene48733.t1 | AT5G39580 | 38.978 | 313 | 181 | 7 | 1 | 309 | 9 | 315 | 5.37e-71 | 223 |
| MS.gene48733.t1 | AT5G64120 | 38.509 | 322 | 178 | 8 | 2 | 309 | 9 | 324 | 1.40e-70 | 222 |
| MS.gene48733.t1 | AT4G37520 | 37.874 | 301 | 181 | 4 | 15 | 309 | 25 | 325 | 1.02e-69 | 219 |
| MS.gene48733.t1 | AT4G11290 | 38.095 | 315 | 188 | 6 | 1 | 309 | 9 | 322 | 1.45e-69 | 219 |
| MS.gene48733.t1 | AT5G67400 | 38.284 | 303 | 181 | 4 | 13 | 309 | 23 | 325 | 1.73e-69 | 219 |
| MS.gene48733.t1 | AT2G41480 | 39.185 | 319 | 176 | 7 | 4 | 309 | 11 | 324 | 1.75e-69 | 219 |
| MS.gene48733.t1 | AT1G05260 | 39.032 | 310 | 182 | 6 | 5 | 309 | 14 | 321 | 3.23e-69 | 218 |
| MS.gene48733.t1 | AT1G71695 | 40.134 | 299 | 166 | 6 | 17 | 309 | 44 | 335 | 3.88e-69 | 219 |
| MS.gene48733.t1 | AT4G37530 | 38.235 | 306 | 183 | 4 | 10 | 309 | 20 | 325 | 8.09e-69 | 217 |
| MS.gene48733.t1 | AT3G01190 | 42.038 | 314 | 167 | 9 | 3 | 309 | 12 | 317 | 1.10e-68 | 217 |
| MS.gene48733.t1 | AT4G37520 | 37.209 | 301 | 180 | 5 | 15 | 309 | 25 | 322 | 7.44e-67 | 212 |
| MS.gene48733.t1 | AT5G15180 | 41.000 | 300 | 164 | 7 | 17 | 309 | 32 | 325 | 1.82e-66 | 211 |
| MS.gene48733.t1 | AT2G34060 | 37.685 | 337 | 176 | 11 | 1 | 309 | 12 | 342 | 3.51e-66 | 211 |
| MS.gene48733.t1 | AT1G05240 | 40.000 | 295 | 168 | 6 | 21 | 309 | 30 | 321 | 4.11e-66 | 210 |
| MS.gene48733.t1 | AT1G05250 | 40.000 | 295 | 168 | 6 | 21 | 309 | 30 | 321 | 4.11e-66 | 210 |
| MS.gene48733.t1 | AT3G21770 | 38.065 | 310 | 183 | 6 | 6 | 309 | 18 | 324 | 3.53e-65 | 208 |
| MS.gene48733.t1 | AT4G30170 | 36.275 | 306 | 177 | 6 | 14 | 309 | 24 | 321 | 2.37e-64 | 206 |
| MS.gene48733.t1 | AT1G77100 | 38.170 | 317 | 179 | 8 | 1 | 309 | 8 | 315 | 7.59e-64 | 204 |
| MS.gene48733.t1 | AT5G14130 | 33.750 | 320 | 186 | 5 | 4 | 309 | 19 | 326 | 1.04e-62 | 202 |
| MS.gene48733.t1 | AT1G30870 | 40.000 | 295 | 163 | 7 | 21 | 309 | 55 | 341 | 1.56e-62 | 202 |
| MS.gene48733.t1 | AT4G17690 | 38.667 | 300 | 172 | 6 | 16 | 309 | 25 | 318 | 2.44e-62 | 201 |
| MS.gene48733.t1 | AT3G28200 | 37.458 | 299 | 174 | 5 | 17 | 309 | 21 | 312 | 2.87e-62 | 200 |
| MS.gene48733.t1 | AT5G14130 | 33.750 | 320 | 186 | 5 | 4 | 309 | 60 | 367 | 4.33e-62 | 201 |
| MS.gene48733.t1 | AT2G18980 | 35.093 | 322 | 182 | 6 | 1 | 309 | 12 | 319 | 6.66e-62 | 199 |
| MS.gene48733.t1 | AT5G47000 | 38.019 | 313 | 183 | 5 | 3 | 309 | 19 | 326 | 6.77e-62 | 200 |
| MS.gene48733.t1 | AT4G25980 | 37.255 | 306 | 180 | 6 | 12 | 309 | 21 | 322 | 1.77e-61 | 198 |
| MS.gene48733.t1 | AT4G37530 | 40.000 | 260 | 152 | 3 | 10 | 265 | 20 | 279 | 1.10e-60 | 195 |
| MS.gene48733.t1 | AT1G24110 | 33.227 | 313 | 201 | 5 | 3 | 309 | 8 | 318 | 3.52e-60 | 195 |
| MS.gene48733.t1 | AT5G40150 | 37.415 | 294 | 174 | 4 | 21 | 309 | 36 | 324 | 5.36e-60 | 194 |
| MS.gene48733.t1 | AT4G26010 | 37.793 | 299 | 169 | 7 | 15 | 309 | 20 | 305 | 8.27e-60 | 194 |
| MS.gene48733.t1 | AT4G26010 | 37.793 | 299 | 169 | 7 | 15 | 309 | 48 | 333 | 1.33e-59 | 194 |
| MS.gene48733.t1 | AT5G64100 | 37.248 | 298 | 169 | 8 | 20 | 309 | 40 | 327 | 1.02e-58 | 191 |
| MS.gene48733.t1 | AT4G33870 | 33.533 | 334 | 177 | 4 | 17 | 309 | 68 | 397 | 1.53e-58 | 193 |
| MS.gene48733.t1 | AT2G39040 | 36.634 | 303 | 180 | 7 | 17 | 309 | 46 | 346 | 2.89e-58 | 191 |
| MS.gene48733.t1 | AT4G21960 | 34.528 | 307 | 185 | 6 | 12 | 309 | 24 | 323 | 1.15e-57 | 189 |
| MS.gene48733.t1 | AT5G64110 | 37.584 | 298 | 174 | 6 | 18 | 309 | 35 | 326 | 1.41e-57 | 188 |
| MS.gene48733.t1 | AT2G24800 | 36.213 | 301 | 182 | 5 | 15 | 309 | 29 | 325 | 6.82e-56 | 184 |
| MS.gene48733.t1 | AT3G17070 | 35.216 | 301 | 184 | 4 | 17 | 309 | 38 | 335 | 7.10e-56 | 184 |
| MS.gene48733.t1 | AT5G22410 | 37.209 | 301 | 174 | 7 | 17 | 309 | 28 | 321 | 1.22e-55 | 183 |
| MS.gene48733.t1 | AT1G34510 | 36.393 | 305 | 165 | 9 | 15 | 309 | 20 | 305 | 1.17e-54 | 180 |
| MS.gene48733.t1 | AT2G35380 | 43.210 | 243 | 129 | 5 | 76 | 309 | 1 | 243 | 1.63e-53 | 175 |
| MS.gene48733.t1 | AT4G31760 | 33.438 | 317 | 196 | 7 | 1 | 309 | 13 | 322 | 8.48e-53 | 176 |
| MS.gene48733.t1 | AT2G37130 | 32.258 | 310 | 195 | 7 | 8 | 309 | 21 | 323 | 1.36e-52 | 176 |
| MS.gene48733.t1 | AT2G37130 | 32.997 | 297 | 184 | 7 | 21 | 309 | 3 | 292 | 5.19e-52 | 173 |
| MS.gene48733.t1 | AT2G43480 | 35.117 | 299 | 178 | 6 | 21 | 309 | 39 | 331 | 1.25e-51 | 173 |
| MS.gene48733.t1 | AT5G24070 | 32.776 | 299 | 185 | 6 | 21 | 309 | 39 | 331 | 1.67e-48 | 165 |
| MS.gene48733.t1 | AT5G39580 | 40.206 | 194 | 111 | 3 | 1 | 194 | 9 | 197 | 4.49e-44 | 150 |
| MS.gene48733.t1 | AT3G17070 | 33.712 | 264 | 163 | 5 | 54 | 309 | 13 | 272 | 5.29e-42 | 146 |
| MS.gene48733.t1 | AT4G25980 | 42.775 | 173 | 95 | 3 | 12 | 184 | 66 | 234 | 1.98e-39 | 140 |
| MS.gene48733.t1 | AT2G24800 | 39.691 | 194 | 107 | 4 | 15 | 202 | 29 | 218 | 4.26e-34 | 125 |
| MS.gene48733.t1 | AT1G77490 | 26.515 | 264 | 147 | 8 | 51 | 297 | 106 | 339 | 3.59e-18 | 84.7 |
| MS.gene48733.t1 | AT1G77490 | 26.515 | 264 | 147 | 8 | 51 | 297 | 106 | 339 | 4.57e-18 | 84.3 |
| MS.gene48733.t1 | AT4G08390 | 29.648 | 199 | 111 | 6 | 110 | 300 | 185 | 362 | 1.50e-14 | 73.9 |
| MS.gene48733.t1 | AT4G08390 | 29.648 | 199 | 111 | 6 | 110 | 300 | 186 | 363 | 1.55e-14 | 73.9 |
| MS.gene48733.t1 | AT4G08390 | 29.648 | 199 | 111 | 6 | 110 | 300 | 186 | 363 | 1.55e-14 | 73.9 |
| MS.gene48733.t1 | AT4G08390 | 29.648 | 199 | 111 | 6 | 110 | 300 | 186 | 363 | 1.55e-14 | 73.9 |
| MS.gene48733.t1 | AT3G42570 | 34.737 | 95 | 61 | 1 | 89 | 182 | 53 | 147 | 9.21e-14 | 68.2 |
Find 55 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCAATTGATCCTGTATTT+AGG | 0.134391 | 4.4:-72399526 | MS.gene48733:CDS |
| ATAACTGATGCAACACTTCT+TGG | 0.239498 | 4.4:+72400944 | None:intergenic |
| TGTTGAAGGAATGTTGAAAT+TGG | 0.241814 | 4.4:-72399291 | MS.gene48733:CDS |
| CCTAATCTCACTTCCCAATT+AGG | 0.252124 | 4.4:+72399764 | None:intergenic |
| CAACCAAGTCCTTGAAATAT+TGG | 0.258540 | 4.4:+72399420 | None:intergenic |
| TGCAATTGATCCTGTATTTA+GGG | 0.261821 | 4.4:-72399525 | MS.gene48733:CDS |
| TGCAGACAGGAGGACCTAAT+TGG | 0.285329 | 4.4:-72399778 | MS.gene48733:intron |
| AACTATGCTTGGAGAGAAAT+TGG | 0.290246 | 4.4:-72400593 | MS.gene48733:CDS |
| TGGACTTGGCATGATATTAC+TGG | 0.322076 | 4.4:+72399706 | None:intergenic |
| GGAAGTGAGATTAGGAAGAT+TGG | 0.383464 | 4.4:-72399756 | MS.gene48733:CDS |
| CTGATAAGCCTGGTGAAGTT+AGG | 0.388834 | 4.4:-72399256 | MS.gene48733:CDS |
| AAGGACTTGGTTGGTGGAAG+GGG | 0.412024 | 4.4:-72399410 | MS.gene48733:CDS |
| TTGTGCTGATATCATAATCA+TGG | 0.414358 | 4.4:-72400482 | MS.gene48733:CDS |
| GTTGAAGGAATGTTGAAATT+GGG | 0.420650 | 4.4:-72399290 | MS.gene48733:CDS |
| CTAAATACAGGATCAATTGC+AGG | 0.420661 | 4.4:+72399527 | None:intergenic |
| CTGACAATTGTTTCAGCTTG+TGG | 0.421262 | 4.4:+72400998 | None:intergenic |
| TGAAGTTGTTGATGAGGTTA+CGG | 0.424842 | 4.4:-72400539 | MS.gene48733:CDS |
| TCAAGTTCTTCCCTAAATAC+AGG | 0.427013 | 4.4:+72399515 | None:intergenic |
| TTTCGATAACCAATATTTCA+AGG | 0.438590 | 4.4:-72399429 | MS.gene48733:CDS |
| CAAGGACTTGGTTGGTGGAA+GGG | 0.442363 | 4.4:-72399411 | MS.gene48733:CDS |
| TTCTAGAGATGCTGTAGCAC+TGG | 0.460212 | 4.4:-72400458 | MS.gene48733:intron |
| GCAGACAGGAGGACCTAATT+GGG | 0.460309 | 4.4:-72399777 | MS.gene48733:intron |
| CCTAATTGGGAAGTGAGATT+AGG | 0.463783 | 4.4:-72399764 | MS.gene48733:CDS |
| TAACCAATATTTCAAGGACT+TGG | 0.464692 | 4.4:-72399423 | MS.gene48733:CDS |
| AGGGTCAGGCTTTCCGCTAC+CGG | 0.465665 | 4.4:+72399547 | None:intergenic |
| CAATTCTTCCTAACTTCACC+AGG | 0.473399 | 4.4:+72399248 | None:intergenic |
| AAAGACCTTGTAGCACTCTC+AGG | 0.475027 | 4.4:-72399629 | MS.gene48733:CDS |
| AGAGCAGTGGCATTAGATCT+TGG | 0.483837 | 4.4:+72399686 | None:intergenic |
| ACAGGATCAATTGCAGGGTC+AGG | 0.489409 | 4.4:+72399533 | None:intergenic |
| TCAAGGACTTGGTTGGTGGA+AGG | 0.501873 | 4.4:-72399412 | MS.gene48733:CDS |
| CAATATTTCAAGGACTTGGT+TGG | 0.502030 | 4.4:-72399419 | MS.gene48733:CDS |
| AATTTCTCTCCAAGCATAGT+TGG | 0.513582 | 4.4:+72400595 | None:intergenic |
| GTGGCATTAGATCTTGGACT+TGG | 0.525131 | 4.4:+72399692 | None:intergenic |
| ATCATTTGAAGTTGTTGATG+AGG | 0.525248 | 4.4:-72400545 | MS.gene48733:CDS |
| GATGTTGATCAAAACAAGAC+AGG | 0.532101 | 4.4:-72399476 | MS.gene48733:CDS |
| ACATCAGCGGATCTTCGACC+TGG | 0.537774 | 4.4:-72401040 | MS.gene48733:CDS |
| TATTTCAAGGACTTGGTTGG+TGG | 0.538804 | 4.4:-72399416 | MS.gene48733:CDS |
| TAATTTGTGAAGGGGTGTGA+TGG | 0.542064 | 4.4:-72400640 | MS.gene48733:intron |
| GCTTTAGAGAATGCTTGTCC+TGG | 0.544782 | 4.4:-72400514 | MS.gene48733:CDS |
| AAATCACAGGAGTTGCATCA+AGG | 0.548807 | 4.4:+72399450 | None:intergenic |
| GATGATACACCAACTATGCT+TGG | 0.551588 | 4.4:-72400604 | MS.gene48733:CDS |
| TTTCGGCTATACAATCAATC+CGG | 0.568350 | 4.4:-72399566 | MS.gene48733:CDS |
| TCCCTTTCATTGCAGACAGG+AGG | 0.569641 | 4.4:-72399788 | MS.gene48733:intron |
| GACTTGCAGTCTGATAAGCC+TGG | 0.576693 | 4.4:-72399266 | MS.gene48733:CDS |
| CTCTCAGGATCACACTCTAT+CGG | 0.590516 | 4.4:-72399614 | MS.gene48733:CDS |
| TATTGGTTATCGAAAATCAC+AGG | 0.593232 | 4.4:+72399437 | None:intergenic |
| CATCAGCGGATCTTCGACCT+GGG | 0.594622 | 4.4:-72401039 | MS.gene48733:CDS |
| CTATACAATCAATCCGGTAG+CGG | 0.596485 | 4.4:-72399560 | MS.gene48733:CDS |
| GTGATCCTGAGAGTGCTACA+AGG | 0.617824 | 4.4:+72399624 | None:intergenic |
| GAAGAGATTAATGAGAGCAG+TGG | 0.625249 | 4.4:+72399673 | None:intergenic |
| TAAATACAGGATCAATTGCA+GGG | 0.640618 | 4.4:+72399528 | None:intergenic |
| TGGAAGGGGATTTCTCAACT+CGG | 0.657592 | 4.4:-72399396 | MS.gene48733:CDS |
| TCAGCACAAGAAACAACACC+AGG | 0.669733 | 4.4:+72400496 | None:intergenic |
| AGTTGTTGATGAGGTTACGG+AGG | 0.670605 | 4.4:-72400536 | MS.gene48733:CDS |
| CATAATCATAGTAACATCAG+CGG | 0.678749 | 4.4:-72401053 | MS.gene48733:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTTAAAAAATAATAATA+GGG | + | chr4.4:72399589-72399608 | None:intergenic | 0.0% |
| !! | TTTATTTAAAAAATAATAAT+AGG | + | chr4.4:72399590-72399609 | None:intergenic | 0.0% |
| !!! | ATAATTGCAATAAAATTTTT+TGG | - | chr4.4:72399533-72399552 | MS.gene48733:CDS | 10.0% |
| !!! | TAATTTTATGATTTATGAAA+AGG | - | chr4.4:72399909-72399928 | MS.gene48733:intron | 10.0% |
| !!! | TGATAAAATAAATCTATTTT+AGG | - | chr4.4:72400154-72400173 | MS.gene48733:intron | 10.0% |
| !!! | TTTATAAAATGATTTTTCTT+TGG | - | chr4.4:72399643-72399662 | MS.gene48733:CDS | 10.0% |
| !! | AATGAAAGGGAAATAAAAAT+AGG | + | chr4.4:72400523-72400542 | None:intergenic | 20.0% |
| !! | ATGAAAGGGAAATAAAAATA+GGG | + | chr4.4:72400522-72400541 | None:intergenic | 20.0% |
| !! | CTAATTTATATGGACAATAT+TGG | + | chr4.4:72400313-72400332 | None:intergenic | 20.0% |
| !! | GATAATGTGTCTAATTTATA+TGG | + | chr4.4:72400323-72400342 | None:intergenic | 20.0% |
| ! | AGCTACAAATTAGATCAATA+TGG | + | chr4.4:72400259-72400278 | None:intergenic | 25.0% |
| ! | ATATGGACAATATTGGAAAA+GGG | + | chr4.4:72400306-72400325 | None:intergenic | 25.0% |
| ! | TATATGGACAATATTGGAAA+AGG | + | chr4.4:72400307-72400326 | None:intergenic | 25.0% |
| ! | TATCGAGAAGAAAATAGATT+TGG | - | chr4.4:72400339-72400358 | MS.gene48733:intron | 25.0% |
| ! | TTTCGATAACCAATATTTCA+AGG | - | chr4.4:72400891-72400910 | MS.gene48733:intron | 25.0% |
| !! | AGCTACATTTTAGATCAATA+TGG | + | chr4.4:72400215-72400234 | None:intergenic | 25.0% |
| !!! | ATGAGTTGTTTAATTTGTGA+AGG | - | chr4.4:72399670-72399689 | MS.gene48733:CDS | 25.0% |
| !!! | GTTGTTTTTCAATCATGTTT+CGG | - | chr4.4:72400737-72400756 | MS.gene48733:intron | 25.0% |
| !!! | TGAGTTGTTTAATTTGTGAA+GGG | - | chr4.4:72399671-72399690 | MS.gene48733:CDS | 25.0% |
| !!! | TTTTTCAAAGCATTTGTTGA+AGG | - | chr4.4:72401015-72401034 | MS.gene48733:CDS | 25.0% |
| ACAGTTTGATCTCGAATTTA+AGG | - | chr4.4:72400397-72400416 | MS.gene48733:intron | 30.0% | |
| CATAATCATAGTAACATCAG+CGG | - | chr4.4:72399267-72399286 | MS.gene48733:CDS | 30.0% | |
| GTTGAAGGAATGTTGAAATT+GGG | - | chr4.4:72401030-72401049 | MS.gene48733:CDS | 30.0% | |
| TAAATACAGGATCAATTGCA+GGG | + | chr4.4:72400795-72400814 | None:intergenic | 30.0% | |
| TAACCAATATTTCAAGGACT+TGG | - | chr4.4:72400897-72400916 | MS.gene48733:intron | 30.0% | |
| TGCAATTGATCCTGTATTTA+GGG | - | chr4.4:72400795-72400814 | MS.gene48733:intron | 30.0% | |
| TGTTGAAGGAATGTTGAAAT+TGG | - | chr4.4:72401029-72401048 | MS.gene48733:CDS | 30.0% | |
| ! | ATCATTTGAAGTTGTTGATG+AGG | - | chr4.4:72399775-72399794 | MS.gene48733:CDS | 30.0% |
| ! | GGATCTCATTAATTTGAGAT+CGG | + | chr4.4:72400469-72400488 | None:intergenic | 30.0% |
| ! | TAACACTGAGCTTGTATTTT+TGG | + | chr4.4:72400672-72400691 | None:intergenic | 30.0% |
| ! | TATTGGTTATCGAAAATCAC+AGG | + | chr4.4:72400886-72400905 | None:intergenic | 30.0% |
| ! | TTGTGCTGATATCATAATCA+TGG | - | chr4.4:72399838-72399857 | MS.gene48733:intron | 30.0% |
| !! | GAGTTGTTTAATTTGTGAAG+GGG | - | chr4.4:72399672-72399691 | MS.gene48733:CDS | 30.0% |
| !! | TCACTTTTCCACAAACAAAA+GGG | - | chr4.4:72400959-72400978 | MS.gene48733:CDS | 30.0% |
| !! | TTCACTTTTCCACAAACAAA+AGG | - | chr4.4:72400958-72400977 | MS.gene48733:CDS | 30.0% |
| AACTATGCTTGGAGAGAAAT+TGG | - | chr4.4:72399727-72399746 | MS.gene48733:CDS | 35.0% | |
| AATTTCTCTCCAAGCATAGT+TGG | + | chr4.4:72399728-72399747 | None:intergenic | 35.0% | |
| ATAACTGATGCAACACTTCT+TGG | + | chr4.4:72399379-72399398 | None:intergenic | 35.0% | |
| CAACCAAGTCCTTGAAATAT+TGG | + | chr4.4:72400903-72400922 | None:intergenic | 35.0% | |
| CTAAATACAGGATCAATTGC+AGG | + | chr4.4:72400796-72400815 | None:intergenic | 35.0% | |
| CTGCAATTGATCCTGTATTT+AGG | - | chr4.4:72400794-72400813 | MS.gene48733:intron | 35.0% | |
| GATGTTGATCAAAACAAGAC+AGG | - | chr4.4:72400844-72400863 | MS.gene48733:intron | 35.0% | |
| TCAAGTTCTTCCCTAAATAC+AGG | + | chr4.4:72400808-72400827 | None:intergenic | 35.0% | |
| TGGAGAAACACACAAAAGAT+TGG | + | chr4.4:72400195-72400214 | None:intergenic | 35.0% | |
| TTTCGGCTATACAATCAATC+CGG | - | chr4.4:72400754-72400773 | MS.gene48733:intron | 35.0% | |
| ! | CAATATTTCAAGGACTTGGT+TGG | - | chr4.4:72400901-72400920 | MS.gene48733:intron | 35.0% |
| ! | CTCATTAATTTGAGATCGGA+TGG | + | chr4.4:72400465-72400484 | None:intergenic | 35.0% |
| ! | TGAAGTTGTTGATGAGGTTA+CGG | - | chr4.4:72399781-72399800 | MS.gene48733:intron | 35.0% |
| !!! | CATGTTTTTGAGTAATACCC+AGG | + | chr4.4:72399301-72399320 | None:intergenic | 35.0% |
| !!! | TTGTTTTGATCAACATCTCG+AGG | + | chr4.4:72400841-72400860 | None:intergenic | 35.0% |
| AAATCACAGGAGTTGCATCA+AGG | + | chr4.4:72400873-72400892 | None:intergenic | 40.0% | |
| ATTTCCCTTTCATTGCAGAC+AGG | - | chr4.4:72400529-72400548 | MS.gene48733:CDS | 40.0% | |
| CAATTCTTCCTAACTTCACC+AGG | + | chr4.4:72401075-72401094 | None:intergenic | 40.0% | |
| CCTAATCTCACTTCCCAATT+AGG | + | chr4.4:72400559-72400578 | None:intergenic | 40.0% | |
| CCTAATTGGGAAGTGAGATT+AGG | - | chr4.4:72400556-72400575 | MS.gene48733:CDS | 40.0% | |
| CTATACAATCAATCCGGTAG+CGG | - | chr4.4:72400760-72400779 | MS.gene48733:intron | 40.0% | |
| CTGACAATTGTTTCAGCTTG+TGG | + | chr4.4:72399325-72399344 | None:intergenic | 40.0% | |
| GAAGAGATTAATGAGAGCAG+TGG | + | chr4.4:72400650-72400669 | None:intergenic | 40.0% | |
| GATGATACACCAACTATGCT+TGG | - | chr4.4:72399716-72399735 | MS.gene48733:CDS | 40.0% | |
| GGAAGTGAGATTAGGAAGAT+TGG | - | chr4.4:72400564-72400583 | MS.gene48733:CDS | 40.0% | |
| TGGACTTGGCATGATATTAC+TGG | + | chr4.4:72400617-72400636 | None:intergenic | 40.0% | |
| TTGAATATGCAGTCTCAGCA+AGG | + | chr4.4:72400496-72400515 | None:intergenic | 40.0% | |
| ! | TAATTTGTGAAGGGGTGTGA+TGG | - | chr4.4:72399680-72399699 | MS.gene48733:CDS | 40.0% |
| ! | TATTTCAAGGACTTGGTTGG+TGG | - | chr4.4:72400904-72400923 | MS.gene48733:intron | 40.0% |
| !! | CTCACAAACCCTTTTGTTTG+TGG | + | chr4.4:72400970-72400989 | None:intergenic | 40.0% |
| AAAGACCTTGTAGCACTCTC+AGG | - | chr4.4:72400691-72400710 | MS.gene48733:intron | 45.0% | |
| AGAGCAGTGGCATTAGATCT+TGG | + | chr4.4:72400637-72400656 | None:intergenic | 45.0% | |
| CTCTCAGGATCACACTCTAT+CGG | - | chr4.4:72400706-72400725 | MS.gene48733:intron | 45.0% | |
| CTGATAAGCCTGGTGAAGTT+AGG | - | chr4.4:72401064-72401083 | MS.gene48733:CDS | 45.0% | |
| GTGGCATTAGATCTTGGACT+TGG | + | chr4.4:72400631-72400650 | None:intergenic | 45.0% | |
| TCAGCACAAGAAACAACACC+AGG | + | chr4.4:72399827-72399846 | None:intergenic | 45.0% | |
| TCCTCCTGTCTGCAATGAAA+GGG | + | chr4.4:72400536-72400555 | None:intergenic | 45.0% | |
| TGGAAGGGGATTTCTCAACT+CGG | - | chr4.4:72400924-72400943 | MS.gene48733:CDS | 45.0% | |
| ! | AGTTGTTGATGAGGTTACGG+AGG | - | chr4.4:72399784-72399803 | MS.gene48733:intron | 45.0% |
| ! | TTCTAGAGATGCTGTAGCAC+TGG | - | chr4.4:72399862-72399881 | MS.gene48733:intron | 45.0% |
| !! | GCTTTAGAGAATGCTTGTCC+TGG | - | chr4.4:72399806-72399825 | MS.gene48733:intron | 45.0% |
| !!! | TTTTTAAATAAAATAATGAT+AGG | - | chr4.4:72399598-72399617 | MS.gene48733:CDS | 5.0% |
| ACAGGATCAATTGCAGGGTC+AGG | + | chr4.4:72400790-72400809 | None:intergenic | 50.0% | |
| ATGCAGTCTCAGCAAGGATC+TGG | + | chr4.4:72400490-72400509 | None:intergenic | 50.0% | |
| GACTTGCAGTCTGATAAGCC+TGG | - | chr4.4:72401054-72401073 | MS.gene48733:CDS | 50.0% | |
| GCAGACAGGAGGACCTAATT+GGG | - | chr4.4:72400543-72400562 | MS.gene48733:CDS | 50.0% | |
| GTCCTCCTGTCTGCAATGAA+AGG | + | chr4.4:72400537-72400556 | None:intergenic | 50.0% | |
| TCCCTTTCATTGCAGACAGG+AGG | - | chr4.4:72400532-72400551 | MS.gene48733:CDS | 50.0% | |
| TGCAGACAGGAGGACCTAAT+TGG | - | chr4.4:72400542-72400561 | MS.gene48733:CDS | 50.0% | |
| !! | AAGGACTTGGTTGGTGGAAG+GGG | - | chr4.4:72400910-72400929 | MS.gene48733:intron | 50.0% |
| !! | CAAGGACTTGGTTGGTGGAA+GGG | - | chr4.4:72400909-72400928 | MS.gene48733:intron | 50.0% |
| !! | GTGATCCTGAGAGTGCTACA+AGG | + | chr4.4:72400699-72400718 | None:intergenic | 50.0% |
| !! | TCAAGGACTTGGTTGGTGGA+AGG | - | chr4.4:72400908-72400927 | MS.gene48733:intron | 50.0% |
| ACATCAGCGGATCTTCGACC+TGG | - | chr4.4:72399280-72399299 | MS.gene48733:CDS | 55.0% | |
| CATCAGCGGATCTTCGACCT+GGG | - | chr4.4:72399281-72399300 | MS.gene48733:CDS | 55.0% | |
| AGGGTCAGGCTTTCCGCTAC+CGG | + | chr4.4:72400776-72400795 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 72399244 | 72401098 | 72399244 | ID=MS.gene48733 |
| chr4.4 | mRNA | 72399244 | 72401098 | 72399244 | ID=MS.gene48733.t1;Parent=MS.gene48733 |
| chr4.4 | exon | 72400913 | 72401098 | 72400913 | ID=MS.gene48733.t1.exon1;Parent=MS.gene48733.t1 |
| chr4.4 | CDS | 72400913 | 72401098 | 72400913 | ID=cds.MS.gene48733.t1;Parent=MS.gene48733.t1 |
| chr4.4 | exon | 72400459 | 72400650 | 72400459 | ID=MS.gene48733.t1.exon2;Parent=MS.gene48733.t1 |
| chr4.4 | CDS | 72400459 | 72400650 | 72400459 | ID=cds.MS.gene48733.t1;Parent=MS.gene48733.t1 |
| chr4.4 | exon | 72399244 | 72399795 | 72399244 | ID=MS.gene48733.t1.exon3;Parent=MS.gene48733.t1 |
| chr4.4 | CDS | 72399244 | 72399795 | 72399244 | ID=cds.MS.gene48733.t1;Parent=MS.gene48733.t1 |
| Gene Sequence |
| Protein sequence |
