AlfalfaGEDB | MS.gene48733

M. sativa cultivar XinJiangDaYe / MS.gene48733

KEGG: K00430
GO: GO:0003674 GO:0003824 GO:0004601 GO:0005488 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0016209 GO:0016491 GO:0016684 GO:0016999 GO:0017001 GO:0017144 GO:0020037 GO:0042737 GO:0042743 GO:0042744 GO:0044237 GO:0044248 GO:0046906 GO:0048037 GO:0050896 GO:0051186 GO:0051187 GO:0055114 GO:0072593 GO:0097159 GO:1901363
PFAM: PF00141.26

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48733.t1	XP_003605420.2	96.8	309	10	0	1	309	12	320	2.80E-167	597.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48733.t1	Q9SJZ2	71.8	309	87	0	1	309	7	315	1.2e-129	464.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48733.t1	G7JKV1	96.8	309	10	0	1	309	12	320	2.0e-167	597.8

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene24236	MS.gene48733	PPI
MS.gene27263	MS.gene48733	PPI
MS.gene48733	MS.gene029047	PPI
MS.gene48733	MS.gene57204	PPI
MS.gene48733	MS.gene20276	PPI
MS.gene48733	MS.gene09575	PPI
MS.gene48733	MS.gene072888	PPI
MS.gene48733	MS.gene35795	PPI
MS.gene48733	MS.gene27001	PPI
MS.gene48733	MS.gene32715	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48733.t1	MTR_4g031140	96.764	309	10	0	1	309	12	320	0.0	617
MS.gene48733.t1	MTR_4g030890	85.667	300	43	0	10	309	93	392	0.0	550
MS.gene48733.t1	MTR_3g094650	46.349	315	156	5	1	309	11	318	1.32e-95	285
MS.gene48733.t1	MTR_8g076820	46.801	297	154	3	17	309	30	326	5.63e-93	279
MS.gene48733.t1	MTR_3g072190	44.937	316	159	5	2	309	6	314	2.55e-92	277
MS.gene48733.t1	MTR_3g466200	45.687	313	157	5	3	309	11	316	1.40e-91	275
MS.gene48733.t1	MTR_2g029800	45.333	300	159	3	15	309	27	326	3.03e-90	273
MS.gene48733.t1	MTR_5g021060	45.455	297	158	3	17	309	30	326	1.03e-89	271
MS.gene48733.t1	MTR_5g074970	45.597	318	153	6	2	309	15	322	3.49e-89	269
MS.gene48733.t1	MTR_2g029740	44.371	302	163	3	13	309	25	326	1.32e-88	269

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48733.t1	AT2G22420	71.845	309	87	0	1	309	7	315	5.69e-171	477
MS.gene48733.t1	AT2G18150	44.410	322	166	4	1	309	11	332	4.74e-95	285
MS.gene48733.t1	AT5G06720	44.654	318	164	4	4	309	12	329	1.58e-93	281
MS.gene48733.t1	AT4G36430	46.128	297	156	3	17	309	30	326	2.15e-92	278
MS.gene48733.t1	AT5G06730	43.533	317	167	4	5	309	14	330	3.51e-92	278
MS.gene48733.t1	AT5G19890	47.667	300	148	4	15	309	28	323	8.86e-92	276
MS.gene48733.t1	AT5G66390	44.781	297	160	3	17	309	33	329	1.16e-90	273
MS.gene48733.t1	AT2G18140	46.980	298	154	3	16	309	34	331	1.69e-90	273
MS.gene48733.t1	AT4G16270	47.099	293	151	4	20	309	53	344	2.33e-88	268
MS.gene48733.t1	AT3G50990	43.478	299	165	3	15	309	39	337	7.97e-87	264

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Find 55 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACCTTGTAGCACTCTC+AGG	0.475027	4.4:-72399629	MS.gene48733:CDS
AAATCACAGGAGTTGCATCA+AGG	0.548807	4.4:+72399450	None:intergenic
AACTATGCTTGGAGAGAAAT+TGG	0.290246	4.4:-72400593	MS.gene48733:CDS
AAGGACTTGGTTGGTGGAAG+GGG	0.412024	4.4:-72399410	MS.gene48733:CDS
AATTTCTCTCCAAGCATAGT+TGG	0.513582	4.4:+72400595	None:intergenic
ACAGGATCAATTGCAGGGTC+AGG	0.489409	4.4:+72399533	None:intergenic
ACATCAGCGGATCTTCGACC+TGG	0.537774	4.4:-72401040	MS.gene48733:CDS
AGAGCAGTGGCATTAGATCT+TGG	0.483837	4.4:+72399686	None:intergenic
AGGGTCAGGCTTTCCGCTAC+CGG	0.465665	4.4:+72399547	None:intergenic
AGTTGTTGATGAGGTTACGG+AGG	0.670605	4.4:-72400536	MS.gene48733:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAGTTTGATCTCGAATTTA+AGG	-	chr4.4:72400397-72400416	MS.gene48733:intron	30.0%
CATAATCATAGTAACATCAG+CGG	-	chr4.4:72399267-72399286	MS.gene48733:CDS	30.0%
GTTGAAGGAATGTTGAAATT+GGG	-	chr4.4:72401030-72401049	MS.gene48733:CDS	30.0%
TAAATACAGGATCAATTGCA+GGG	+	chr4.4:72400795-72400814	None:intergenic	30.0%
TAACCAATATTTCAAGGACT+TGG	-	chr4.4:72400897-72400916	MS.gene48733:intron	30.0%
TGCAATTGATCCTGTATTTA+GGG	-	chr4.4:72400795-72400814	MS.gene48733:intron	30.0%
TGTTGAAGGAATGTTGAAAT+TGG	-	chr4.4:72401029-72401048	MS.gene48733:CDS	30.0%
AACTATGCTTGGAGAGAAAT+TGG	-	chr4.4:72399727-72399746	MS.gene48733:CDS	35.0%
AATTTCTCTCCAAGCATAGT+TGG	+	chr4.4:72399728-72399747	None:intergenic	35.0%
ATAACTGATGCAACACTTCT+TGG	+	chr4.4:72399379-72399398	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	72399244	72401098	72399244	ID=MS.gene48733
chr4.4	mRNA	72399244	72401098	72399244	ID=MS.gene48733.t1;Parent=MS.gene48733
chr4.4	exon	72400913	72401098	72400913	ID=MS.gene48733.t1.exon1;Parent=MS.gene48733.t1
chr4.4	CDS	72400913	72401098	72400913	ID=cds.MS.gene48733.t1;Parent=MS.gene48733.t1
chr4.4	exon	72400459	72400650	72400459	ID=MS.gene48733.t1.exon2;Parent=MS.gene48733.t1
chr4.4	CDS	72400459	72400650	72400459	ID=cds.MS.gene48733.t1;Parent=MS.gene48733.t1
chr4.4	exon	72399244	72399795	72399244	ID=MS.gene48733.t1.exon3;Parent=MS.gene48733.t1
chr4.4	CDS	72399244	72399795	72399244	ID=cds.MS.gene48733.t1;Parent=MS.gene48733.t1

Gene Sequence

>MS.gene48733

ATGTTTCTGATCTTCATCATAAACATAATCATAGTAACATCAGCGGATCTTCGACCTGGGTATTACTCAAAAACATGTCCACAAGCTGAAACAATTGTCAGAGATGTTATGAGAAATGCTCTTAAAAAAGAACCAAGAAGTGTTGCATCAGTTATGAGATTTCAATTTCATGATTGCTTTGTTAATGTATGTTCTCTCTTTACTCTTTCTTACAATCAAAATTCTATGTTTTCAATCAGTTATTTTTTAGATTTATATATCTCTTTAATCTCTACAAATATTTTGTCACATAATTGCAATAAAATTTTTTGGTCAAAGATTTAAAATAAAATATGTAAAGCGCCCTATTATTATTTTTTAAATAAAATAATGATAGGAATTAAAATATATTTATTTTTATTTATAAAATGATTTTTCTTTGGCTGAATGAGTTGTTTAATTTGTGAAGGGGTGTGATGGTTCTGTGTTGCTTGATGATACACCAACTATGCTTGGAGAGAAATTGGCTCTTTCAAATATAAATTCACTCAGATCATTTGAAGTTGTTGATGAGGTTACGGAGGCTTTAGAGAATGCTTGTCCTGGTGTTGTTTCTTGTGCTGATATCATAATCATGGCTTCTAGAGATGCTGTAGCACTGGTTTGTATTTCTCAATATCTTACAATAATTTTATGATTTATGAAAAGGAAAAAAAAATTTACTAATGAATTTGACTCTTTTTTATTGTTTGTTTATTTACTCTTCATTATTTAAAAAAAATAAATATGACTCTTCTAGAAAATCAATAATTAGTATTTTAATTTTTTATTGTATGTGCACATAATATTTACAATTGATTTATTAGTATTGCAAAAAAAAAAATTGATTTATTAGTTTGACAGTTGAGATTATTTATTTTATCACAGAAAGTGATAAAATAAATCTATTTTAGGTTTTCAAAATTATCACCAATCTTTTGTGTGTTTCTCCATATTGATCTAAAATGTAGCTAATTTGTAGCTAAAATGTTTACCATATTGATCTAATTTGTAGCTATCTTTTTTTGTTGAATTATTTTTCCCTTTTCCAATATTGTCCATATAAATTAGACACATTATCGAGAAGAAAATAGATTTGGTGCAAAAATTGTGTCGCATATAGTTTTTATTCTCAACAGTTTGATCTCGAATTTAAGGACGACGTCTTTTAAATCTTATTTTGTTATTGTGTAATCTGAGCCATCCGATCTCAAATTAATGAGATCCAGATCCTTGCTGAGACTGCATATTCAAATTCCCTATTTTTATTTCCCTTTCATTGCAGACAGGAGGACCTAATTGGGAAGTGAGATTAGGAAGATTGGACAGCTTAACAGCAAGTCAAGATAACTCCAGTAATATCATGCCAAGTCCAAGATCTAATGCCACTGCTCTCATTAATCTCTTCCAAAAATACAAGCTCAGTGTTAAAGACCTTGTAGCACTCTCAGGATCACACTCTATCGGCAAAGCTCGTTGTTTTTCAATCATGTTTCGGCTATACAATCAATCCGGTAGCGGAAAGCCTGACCCTGCAATTGATCCTGTATTTAGGGAAGAACTTGACAAGCTATGTCCTCGAGATGTTGATCAAAACAAGACAGGAAACCTTGATGCAACTCCTGTGATTTTCGATAACCAATATTTCAAGGACTTGGTTGGTGGAAGGGGATTTCTCAACTCGGATCAAACACTTTTCACTTTTCCACAAACAAAAGGGTTTGTGAGTTTGTTTAGCGAAGATCAAAGTGAGTTTTTCAAAGCATTTGTTGAAGGAATGTTGAAATTGGGTGACTTGCAGTCTGATAAGCCTGGTGAAGTTAGGAAGAATTGTTGA

Protein sequence

>MS.gene48733.t1

MFLIFIINIIIVTSADLRPGYYSKTCPQAETIVRDVMRNALKKEPRSVASVMRFQFHDCFVNGCDGSVLLDDTPTMLGEKLALSNINSLRSFEVVDEVTEALENACPGVVSCADIIIMASRDAVALTGGPNWEVRLGRLDSLTASQDNSSNIMPSPRSNATALINLFQKYKLSVKDLVALSGSHSIGKARCFSIMFRLYNQSGSGKPDPAIDPVFREELDKLCPRDVDQNKTGNLDATPVIFDNQYFKDLVGGRGFLNSDQTLFTFPQTKGFVSLFSEDQSEFFKAFVEGMLKLGDLQSDKPGEVRKNC