AlfalfaGEDB | MS.gene51441

M. sativa cultivar XinJiangDaYe / MS.gene51441

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51441.t1	XP_013468641.1	100	126	0	0	1	126	1	126	1.70E-65	258.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51441.t1	Q8L8Q8	83.2	125	21	0	1	125	1	125	1.7e-58	226.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51441.t1	A0A072VML2	100.0	126	0	0	1	126	1	126	1.2e-65	258.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049260	MS.gene51441	0.808603	2.86E-50	-1.69E-46
MS.gene049821	MS.gene51441	0.803376	3.59E-49	-1.69E-46
MS.gene050020	MS.gene51441	0.838712	2.44E-57	-1.69E-46
MS.gene050303	MS.gene51441	0.823525	1.32E-53	-1.69E-46
MS.gene050438	MS.gene51441	0.814946	1.19E-51	-1.69E-46
MS.gene050719	MS.gene51441	0.811386	7.18E-51	-1.69E-46
MS.gene050721	MS.gene51441	0.806749	7.07E-50	-1.69E-46
MS.gene050724	MS.gene51441	0.812507	4.10E-51	-1.69E-46
MS.gene050750	MS.gene51441	0.815984	6.98E-52	-1.69E-46
MS.gene050807	MS.gene51441	0.804135	2.50E-49	-1.69E-46
MS.gene050953	MS.gene51441	0.807254	5.53E-50	-1.69E-46
MS.gene051265	MS.gene51441	0.80114	1.04E-48	-1.69E-46
MS.gene053054	MS.gene51441	-0.812974	3.24E-51	-1.69E-46
MS.gene053074	MS.gene51441	0.808307	3.30E-50	-1.69E-46
MS.gene053226	MS.gene51441	0.806136	9.53E-50	-1.69E-46
MS.gene053630	MS.gene51441	0.81469	1.36E-51	-1.69E-46
MS.gene054833	MS.gene51441	0.802773	4.79E-49	-1.69E-46
MS.gene055358	MS.gene51441	0.801743	7.80E-49	-1.69E-46
MS.gene055608	MS.gene51441	0.807731	4.38E-50	-1.69E-46
MS.gene055818	MS.gene51441	0.808731	2.68E-50	-1.69E-46
MS.gene056173	MS.gene51441	0.807762	4.31E-50	-1.69E-46
MS.gene056498	MS.gene51441	0.830115	3.52E-55	-1.69E-46
MS.gene057060	MS.gene51441	0.851758	7.20E-61	-1.69E-46
MS.gene057260	MS.gene51441	0.809211	2.12E-50	-1.69E-46
MS.gene057314	MS.gene51441	0.803668	3.12E-49	-1.69E-46
MS.gene057414	MS.gene51441	0.804524	2.07E-49	-1.69E-46
MS.gene058168	MS.gene51441	0.812406	4.31E-51	-1.69E-46
MS.gene059006	MS.gene51441	0.818973	1.48E-52	-1.69E-46
MS.gene05954	MS.gene51441	0.82417	9.33E-54	-1.69E-46
MS.gene059569	MS.gene51441	0.851443	8.85E-61	-1.69E-46
MS.gene060137	MS.gene51441	-0.804672	1.93E-49	-1.69E-46
MS.gene060429	MS.gene51441	0.837516	4.96E-57	-1.69E-46
MS.gene060572	MS.gene51441	0.82898	6.65E-55	-1.69E-46
MS.gene060634	MS.gene51441	-0.802309	5.97E-49	-1.69E-46
MS.gene060803	MS.gene51441	0.836106	1.14E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51441.t1	MTR_1g073620	100.000	126	0	0	1	126	1	126	3.98e-90	256

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51441.t1	AT5G64816	83.333	120	20	1	120	1	120	1.44e-74	218
MS.gene51441.t1	AT5G64816	83.333	120	20	1	120	1	120	1.44e-74	218
MS.gene51441.t1	AT5G64816	83.333	120	20	1	120	1	120	1.44e-74	218
MS.gene51441.t1	AT5G64816	83.333	120	20	1	120	1	120	1.44e-74	218

Find 23 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATATTTGGTGGTCACTTT+TGG	0.138181	1.3:-53051704	MS.gene51441:CDS
TTCAAAGAGGATGTTGAAAA+AGG	0.272946	1.3:-53051545	MS.gene51441:CDS
ACCAACATCAAGTACCTAAT+TGG	0.307555	1.3:-53051417	MS.gene51441:CDS
GATACTTGTGTTACTGTTTG+TGG	0.347747	1.3:-53051493	MS.gene51441:CDS
GCAGTCCCTGTCGTTGTTGC+AGG	0.348680	1.3:-53051676	MS.gene51441:CDS
GTGCAGAGATGGGAGATATT+TGG	0.371563	1.3:-53051717	None:intergenic
ATTGGAATGACACATGTCTT+AGG	0.381625	1.3:-53051399	MS.gene51441:CDS
AGACATGTGTCATTCCAATT+AGG	0.386451	1.3:+53051403	None:intergenic
GTAACACAAGTATCAGGAAT+TGG	0.406394	1.3:+53051502	None:intergenic
CAAACAGTAACACAAGTATC+AGG	0.430522	1.3:+53051496	None:intergenic
GCTTGTCCTGCAACAACGAC+AGG	0.451940	1.3:+53051670	None:intergenic
TCCAATTAGGTACTTGATGT+TGG	0.474351	1.3:+53051416	None:intergenic
AAGCATTTAGAGTTAAGAAA+AGG	0.483524	1.3:-53051651	MS.gene51441:CDS
TGCTGAAGCTTGTGCTCGTA+CGG	0.484272	1.3:-53051449	MS.gene51441:CDS
AGCAAAGGTTGAAGAGTGCA+AGG	0.529370	1.3:-53051618	MS.gene51441:CDS
AGAGTGCAAGGGGAAGAGAA+AGG	0.552353	1.3:-53051606	MS.gene51441:CDS
AAAGGAATGCAGAAGAGCAA+AGG	0.565930	1.3:-53051633	MS.gene51441:CDS
CAAAGGTTGAAGAGTGCAAG+GGG	0.608201	1.3:-53051616	MS.gene51441:CDS
GCAAAGGTTGAAGAGTGCAA+GGG	0.610878	1.3:-53051617	MS.gene51441:CDS
CTTGTCCTGCAACAACGACA+GGG	0.623317	1.3:+53051671	None:intergenic
GGAATGACACATGTCTTAGG+AGG	0.635618	1.3:-53051396	MS.gene51441:CDS
AAAGGGTCTGCACTTCAAAG+AGG	0.655293	1.3:-53051558	MS.gene51441:CDS
CAGAGATGGGAGATATTTGG+TGG	0.699481	1.3:-53051714	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGCATTTAGAGTTAAGAAA+AGG	-	chr1.3:53051409-53051428	MS.gene51441:CDS	25.0%
!!	ATGAAATTTTCGTTTGTGAA+AGG	-	chr1.3:53051484-53051503	MS.gene51441:CDS	25.0%
!!	TGAAATTTTCGTTTGTGAAA+GGG	-	chr1.3:53051485-53051504	MS.gene51441:CDS	25.0%
	TTCAAAGAGGATGTTGAAAA+AGG	-	chr1.3:53051515-53051534	MS.gene51441:CDS	30.0%
	AAGAGGATGTTGAAAAAGGT+TGG	-	chr1.3:53051519-53051538	MS.gene51441:CDS	35.0%
	ACCAACATCAAGTACCTAAT+TGG	-	chr1.3:53051643-53051662	MS.gene51441:CDS	35.0%
	ATTGGAATGACACATGTCTT+AGG	-	chr1.3:53051661-53051680	MS.gene51441:CDS	35.0%
	CAAACAGTAACACAAGTATC+AGG	+	chr1.3:53051567-53051586	None:intergenic	35.0%
	GTAACACAAGTATCAGGAAT+TGG	+	chr1.3:53051561-53051580	None:intergenic	35.0%
	TCCAATTAGGTACTTGATGT+TGG	+	chr1.3:53051647-53051666	None:intergenic	35.0%
!	AGACATGTGTCATTCCAATT+AGG	+	chr1.3:53051660-53051679	None:intergenic	35.0%
!	GATACTTGTGTTACTGTTTG+TGG	-	chr1.3:53051567-53051586	MS.gene51441:CDS	35.0%
!!	AGATATTTGGTGGTCACTTT+TGG	-	chr1.3:53051356-53051375	MS.gene51441:CDS	35.0%
!!!	ATATTTGGTGGTCACTTTTG+GGG	-	chr1.3:53051358-53051377	MS.gene51441:CDS	35.0%
!!!	GATATTTGGTGGTCACTTTT+GGG	-	chr1.3:53051357-53051376	MS.gene51441:CDS	35.0%
	AAAGGAATGCAGAAGAGCAA+AGG	-	chr1.3:53051427-53051446	MS.gene51441:CDS	40.0%
!!!	TATTTGGTGGTCACTTTTGG+GGG	-	chr1.3:53051359-53051378	MS.gene51441:CDS	40.0%
	AAAGGGTCTGCACTTCAAAG+AGG	-	chr1.3:53051502-53051521	MS.gene51441:CDS	45.0%
	GGAATGACACATGTCTTAGG+AGG	-	chr1.3:53051664-53051683	MS.gene51441:CDS	45.0%
!	AGCAAAGGTTGAAGAGTGCA+AGG	-	chr1.3:53051442-53051461	MS.gene51441:CDS	45.0%
!	CAAAGGTTGAAGAGTGCAAG+GGG	-	chr1.3:53051444-53051463	MS.gene51441:CDS	45.0%
!	GCAAAGGTTGAAGAGTGCAA+GGG	-	chr1.3:53051443-53051462	MS.gene51441:CDS	45.0%
	AGAGTGCAAGGGGAAGAGAA+AGG	-	chr1.3:53051454-53051473	MS.gene51441:CDS	50.0%
	CTTGTCCTGCAACAACGACA+GGG	+	chr1.3:53051392-53051411	None:intergenic	50.0%
	TGCTGAAGCTTGTGCTCGTA+CGG	-	chr1.3:53051611-53051630	MS.gene51441:CDS	50.0%
	GCTTGTCCTGCAACAACGAC+AGG	+	chr1.3:53051393-53051412	None:intergenic	55.0%
	GCAGTCCCTGTCGTTGTTGC+AGG	-	chr1.3:53051384-53051403	MS.gene51441:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	53051351	53051731	53051351	ID=MS.gene51441
chr1.3	mRNA	53051351	53051731	53051351	ID=MS.gene51441.t1;Parent=MS.gene51441
chr1.3	exon	53051351	53051731	53051351	ID=MS.gene51441.t1.exon1;Parent=MS.gene51441.t1
chr1.3	CDS	53051351	53051731	53051351	ID=cds.MS.gene51441.t1;Parent=MS.gene51441.t1

Gene Sequence

>MS.gene51441

ATGGGAGATATTTGGTGGTCACTTTTGGGGGCAGCAGTCCCTGTCGTTGTTGCAGGACAAGCATTTAGAGTTAAGAAAAGGAATGCAGAAGAGCAAAGGTTGAAGAGTGCAAGGGGAAGAGAAAGGAGTTCTGATGAAATTTTCGTTTGTGAAAGGGTCTGCACTTCAAAGAGGATGTTGAAAAAGGTTGGCTCTTTCTCAAAAGATCCAATTCCTGATACTTGTGTTACTGTTTGTGGTGTGTCTGATCTTGACGCGTGTGCTGAAGCTTGTGCTCGTACGGTTTGTGTTAACCAACATCAAGTACCTAATTGGAATGACACATGTCTTAGGAGGTGTCAGAGTGAATGCTTGAAACTCTCTTCTCAGTCTTCTCAGTAG

Protein sequence

>MS.gene51441.t1

MGDIWWSLLGAAVPVVVAGQAFRVKKRNAEEQRLKSARGRERSSDEIFVCERVCTSKRMLKKVGSFSKDPIPDTCVTVCGVSDLDACAEACARTVCVNQHQVPNWNDTCLRRCQSECLKLSSQSSQ