AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054833


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTGAGGAAAACATAAAA+TGG 0.213796 7.3:+19726095 MS.gene054833:CDS
GGTGACAGATCCAGTGATTT+TGG 0.292587 7.3:+19726703 MS.gene054833:intron
GTATAGTGTCATTGGTCATT+TGG 0.367045 7.3:+19726535 MS.gene054833:CDS
CTTTATCTTTCTCTTGAGAA+TGG 0.369439 7.3:+19725423 MS.gene054833:CDS
CATTGCCAATGTCACAATAA+AGG 0.376211 7.3:+19726581 MS.gene054833:CDS
GAACAAGGAAATGAAATAGA+AGG 0.401759 7.3:+19726791 MS.gene054833:CDS
TTACCTCACGGTTATAAACT+TGG 0.402760 7.3:-19725464 None:intergenic
TGCTTAGATGACTTAAGGCC+TGG 0.434332 7.3:+19726175 MS.gene054833:CDS
AGAAACAAGGAGTTCCCTTA+TGG 0.448940 7.3:+19726223 MS.gene054833:CDS
TTACAAAGTGGAAGAAACTT+TGG 0.456123 7.3:+19726855 MS.gene054833:CDS
CTGACTGCTTAGATGACTTA+AGG 0.460389 7.3:+19726170 MS.gene054833:CDS
GGTTGGTGGTATAGTGTCAT+TGG 0.469917 7.3:+19726527 MS.gene054833:intron
AGAGCAGCAATTGAGTCATC+TGG 0.473004 7.3:-19725402 None:intergenic
TCTTACCTTTATTGTGACAT+TGG 0.477470 7.3:-19726586 None:intergenic
TTGCACATACCTTGCTTGAA+AGG 0.477849 7.3:-19725695 None:intergenic
AACTGAGGAAAACATAAAAT+GGG 0.478038 7.3:+19726096 MS.gene054833:CDS
ACATGTGAAGAAGAGTCAAT+AGG 0.486449 7.3:-19726136 None:intergenic
TCACGGTTATAAACTTGGGC+AGG 0.488493 7.3:-19725459 None:intergenic
TGAATTTCAACATGATCTCC+AGG 0.504590 7.3:-19726193 None:intergenic
CTTTATTGTGACATTGGCAA+TGG 0.507841 7.3:-19726580 None:intergenic
TTGGAAACATGTCAAGGTGA+TGG 0.516906 7.3:+19726554 MS.gene054833:CDS
TGGAAACATGTCAAGGTGAT+GGG 0.523680 7.3:+19726555 MS.gene054833:CDS
TGTGTGAACTCCAAAATCAC+TGG 0.525464 7.3:-19726713 None:intergenic
TCTGACAGGTACTCACCTCA+TGG 0.532267 7.3:+19726064 MS.gene054833:intron
TACCTCACGGTTATAAACTT+GGG 0.540506 7.3:-19725463 None:intergenic
GAGTTCACACAATACACAGC+TGG 0.557018 7.3:+19726725 MS.gene054833:CDS
GGTACTCACCTCATGGGAGA+TGG 0.562172 7.3:+19726071 MS.gene054833:intron
TAGGATGAGACATACCATAA+GGG 0.562365 7.3:-19726237 None:intergenic
AATACACAGCTGGCTCAAGA+TGG 0.566869 7.3:+19726735 MS.gene054833:CDS
GAGATCATGTTGAAATTCAG+TGG 0.569671 7.3:+19726197 MS.gene054833:CDS
GAACAGATGCCTTTCAAGCA+AGG 0.573120 7.3:+19725686 MS.gene054833:CDS
AGGAAAACATAAAATGGGAA+AGG 0.579315 7.3:+19726101 MS.gene054833:CDS
CACGGTTATAAACTTGGGCA+GGG 0.616974 7.3:-19725458 None:intergenic
GGTCATTTGGAAACATGTCA+AGG 0.617542 7.3:+19726548 MS.gene054833:CDS
AATCAGAAAGTTGCATAGCA+AGG 0.629679 7.3:+19726826 MS.gene054833:CDS
CTGACAGGTACTCACCTCAT+GGG 0.633290 7.3:+19726065 MS.gene054833:intron
AATTCAGTGGAGAAGAAACA+AGG 0.638341 7.3:+19726210 MS.gene054833:CDS
TCAGTTGTCCATCTCCCATG+AGG 0.638779 7.3:-19726079 None:intergenic
CAGAAAGTTGCATAGCAAGG+AGG 0.668496 7.3:+19726829 MS.gene054833:CDS
GCAAGGAGGAGATTACAAAG+TGG 0.681205 7.3:+19726843 MS.gene054833:CDS
TGTGAAGAAGAGTCAATAGG+TGG 0.697492 7.3:-19726133 None:intergenic
TGCCCAAGTTTATAACCGTG+AGG 0.709466 7.3:+19725461 MS.gene054833:CDS
TCATGGGAGATGGACAACTG+AGG 0.731418 7.3:+19726081 MS.gene054833:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATACTAATTTTTAAATCA+GGG - chr7.3:19725537-19725556 None:intergenic 10.0%
!!! TTAATACTAATTTTTAAATC+AGG - chr7.3:19725538-19725557 None:intergenic 10.0%
!!! TTTTAAAAATGTTTTGTTAT+AGG + chr7.3:19726506-19726525 MS.gene054833:intron 10.0%
!!! AATATTTTGTCTTGTAATTA+AGG + chr7.3:19725560-19725579 MS.gene054833:intron 15.0%
!!! ATATTTTGTCTTGTAATTAA+GGG + chr7.3:19725561-19725580 MS.gene054833:intron 15.0%
!!! TCAATAGTTTAACATTTTAT+AGG + chr7.3:19725994-19726013 MS.gene054833:intron 15.0%
!!! TTGATAATAGTTAATATTCT+AGG - chr7.3:19726368-19726387 None:intergenic 15.0%
!! AAAGTGACAACATAATTTAA+GGG - chr7.3:19726651-19726670 None:intergenic 20.0%
!! AATAACCTATGAAATACTAA+AGG + chr7.3:19725810-19725829 MS.gene054833:intron 20.0%
!! ATAATTAAAATGTATGCTAG+TGG - chr7.3:19726616-19726635 None:intergenic 20.0%
!! GATGATTAAATCAATAATGT+TGG + chr7.3:19726682-19726701 MS.gene054833:intron 20.0%
!! TAAAGTGACAACATAATTTA+AGG - chr7.3:19726652-19726671 None:intergenic 20.0%
!! TTATGTCAGTAGATAAATAA+AGG + chr7.3:19726328-19726347 MS.gene054833:intron 20.0%
!!! AAAAATGTTTTGTTATAGGT+TGG + chr7.3:19726510-19726529 MS.gene054833:intron 20.0%
!!! AATGAAATAGAAGGTTTTTA+TGG + chr7.3:19726800-19726819 MS.gene054833:CDS 20.0%
!!! AATTAAACCAAAAGTACAAT+AGG - chr7.3:19725780-19725799 None:intergenic 20.0%
!!! ATGAAATAGAAGGTTTTTAT+GGG + chr7.3:19726801-19726820 MS.gene054833:CDS 20.0%
!!! TTTTAAGTACATTTTCAGAA+TGG + chr7.3:19725610-19725629 MS.gene054833:intron 20.0%
! AACTGAGGAAAACATAAAAT+GGG + chr7.3:19726096-19726115 MS.gene054833:CDS 25.0%
! AAGTGACAACATAATTTAAG+GGG - chr7.3:19726650-19726669 None:intergenic 25.0%
! AGAAAAAATCATAGAGAACA+AGG + chr7.3:19726776-19726795 MS.gene054833:CDS 25.0%
! CATTCAATATTGTTGAGTTT+AGG + chr7.3:19725727-19725746 MS.gene054833:intron 25.0%
! TATCAATGTTTGTGATAGAT+TGG + chr7.3:19726382-19726401 MS.gene054833:intron 25.0%
!! TGAAATAGAAGGTTTTTATG+GGG + chr7.3:19726802-19726821 MS.gene054833:CDS 25.0%
AGGAAAACATAAAATGGGAA+AGG + chr7.3:19726101-19726120 MS.gene054833:CDS 30.0%
AGGTAAAAAGACAAGTTGTT+TGG - chr7.3:19725755-19725774 None:intergenic 30.0%
CAACTGAGGAAAACATAAAA+TGG + chr7.3:19726095-19726114 MS.gene054833:CDS 30.0%
CTTTATCTTTCTCTTGAGAA+TGG + chr7.3:19725423-19725442 MS.gene054833:CDS 30.0%
GAACAAGGAAATGAAATAGA+AGG + chr7.3:19726791-19726810 MS.gene054833:CDS 30.0%
GGTATCCTTTAGTATTTCAT+AGG - chr7.3:19725818-19725837 None:intergenic 30.0%
GTCAGTAGATAAATAAAGGT+CGG + chr7.3:19726332-19726351 MS.gene054833:intron 30.0%
TCTTACCTTTATTGTGACAT+TGG - chr7.3:19726589-19726608 None:intergenic 30.0%
TTACAAAGTGGAAGAAACTT+TGG + chr7.3:19726855-19726874 MS.gene054833:CDS 30.0%
TTAGATATTGCACAACAACT+AGG - chr7.3:19726259-19726278 None:intergenic 30.0%
TTTATGCGACTATTTAGAAG+AGG - chr7.3:19726305-19726324 None:intergenic 30.0%
! CTCTTGAGAATGGAAATTTT+TGG + chr7.3:19725433-19725452 MS.gene054833:CDS 30.0%
! GAAATAGAAGGTTTTTATGG+GGG + chr7.3:19726803-19726822 MS.gene054833:CDS 30.0%
!! AATGTTTTGTTATAGGTTGG+TGG + chr7.3:19726513-19726532 MS.gene054833:intron 30.0%
!!! TCATTCATGTTTTTTCTGAC+AGG + chr7.3:19726050-19726069 MS.gene054833:intron 30.0%
AATCAGAAAGTTGCATAGCA+AGG + chr7.3:19726826-19726845 MS.gene054833:CDS 35.0%
AATTCAGTGGAGAAGAAACA+AGG + chr7.3:19726210-19726229 MS.gene054833:CDS 35.0%
ACATGTGAAGAAGAGTCAAT+AGG - chr7.3:19726139-19726158 None:intergenic 35.0%
ACTTCAGACCAAAATCATTC+TGG - chr7.3:19725945-19725964 None:intergenic 35.0%
CATTGCCAATGTCACAATAA+AGG + chr7.3:19726581-19726600 MS.gene054833:CDS 35.0%
CTTTATTGTGACATTGGCAA+TGG - chr7.3:19726583-19726602 None:intergenic 35.0%
GAGATCATGTTGAAATTCAG+TGG + chr7.3:19726197-19726216 MS.gene054833:CDS 35.0%
TACCTCACGGTTATAAACTT+GGG - chr7.3:19725466-19725485 None:intergenic 35.0%
TAGGATGAGACATACCATAA+GGG - chr7.3:19726240-19726259 None:intergenic 35.0%
TATAACCGTGAGGTAAATAC+TGG + chr7.3:19725471-19725490 MS.gene054833:intron 35.0%
TGAATTTCAACATGATCTCC+AGG - chr7.3:19726196-19726215 None:intergenic 35.0%
TTACCTCACGGTTATAAACT+TGG - chr7.3:19725467-19725486 None:intergenic 35.0%
! AACCAAAAGTACAATAGGAC+AGG - chr7.3:19725775-19725794 None:intergenic 35.0%
! GTATAGTGTCATTGGTCATT+TGG + chr7.3:19726535-19726554 MS.gene054833:CDS 35.0%
! TACCTGTCCTATTGTACTTT+TGG + chr7.3:19725770-19725789 MS.gene054833:intron 35.0%
! TAGTGTCTACGTGTTAGTTT+CGG - chr7.3:19725851-19725870 None:intergenic 35.0%
AGAAACAAGGAGTTCCCTTA+TGG + chr7.3:19726223-19726242 MS.gene054833:CDS 40.0%
CTAGGATGAGACATACCATA+AGG - chr7.3:19726241-19726260 None:intergenic 40.0%
CTGACTGCTTAGATGACTTA+AGG + chr7.3:19726170-19726189 MS.gene054833:CDS 40.0%
GGTCATTTGGAAACATGTCA+AGG + chr7.3:19726548-19726567 MS.gene054833:CDS 40.0%
TGCAACCAGTATTTACCTCA+CGG - chr7.3:19725479-19725498 None:intergenic 40.0%
TGGAAACATGTCAAGGTGAT+GGG + chr7.3:19726555-19726574 MS.gene054833:CDS 40.0%
TGTGAAGAAGAGTCAATAGG+TGG - chr7.3:19726136-19726155 None:intergenic 40.0%
TGTGTGAACTCCAAAATCAC+TGG - chr7.3:19726716-19726735 None:intergenic 40.0%
TTGCACATACCTTGCTTGAA+AGG - chr7.3:19725698-19725717 None:intergenic 40.0%
TTGGAAACATGTCAAGGTGA+TGG + chr7.3:19726554-19726573 MS.gene054833:CDS 40.0%
! CATTTTCAGAATGGTCATGC+TGG + chr7.3:19725619-19725638 MS.gene054833:intron 40.0%
! GTCAGACACCAGAATGATTT+TGG + chr7.3:19725934-19725953 MS.gene054833:intron 40.0%
!! TACTGGTTGCATGCAGATTT+TGG + chr7.3:19725488-19725507 MS.gene054833:intron 40.0%
AGAGCAGCAATTGAGTCATC+TGG - chr7.3:19725405-19725424 None:intergenic 45.0%
ATCATTCTGGTGTCTGACAC+TGG - chr7.3:19725932-19725951 None:intergenic 45.0%
CACGGTTATAAACTTGGGCA+GGG - chr7.3:19725461-19725480 None:intergenic 45.0%
CAGAAAGTTGCATAGCAAGG+AGG + chr7.3:19726829-19726848 MS.gene054833:CDS 45.0%
GAACAGATGCCTTTCAAGCA+AGG + chr7.3:19725686-19725705 MS.gene054833:CDS 45.0%
GAGTTCACACAATACACAGC+TGG + chr7.3:19726725-19726744 MS.gene054833:CDS 45.0%
GCAAGGAGGAGATTACAAAG+TGG + chr7.3:19726843-19726862 MS.gene054833:CDS 45.0%
GCACATGACACTAACACATG+TGG - chr7.3:19725910-19725929 None:intergenic 45.0%
TCACGGTTATAAACTTGGGC+AGG - chr7.3:19725462-19725481 None:intergenic 45.0%
TGCCCAAGTTTATAACCGTG+AGG + chr7.3:19725461-19725480 MS.gene054833:CDS 45.0%
TGCTTAGATGACTTAAGGCC+TGG + chr7.3:19726175-19726194 MS.gene054833:CDS 45.0%
! AATACACAGCTGGCTCAAGA+TGG + chr7.3:19726735-19726754 MS.gene054833:CDS 45.0%
! GGTGACAGATCCAGTGATTT+TGG + chr7.3:19726703-19726722 MS.gene054833:intron 45.0%
!! GGTTGGTGGTATAGTGTCAT+TGG + chr7.3:19726527-19726546 MS.gene054833:intron 45.0%
CTGACAGGTACTCACCTCAT+GGG + chr7.3:19726065-19726084 MS.gene054833:intron 50.0%
TCAGTTGTCCATCTCCCATG+AGG - chr7.3:19726082-19726101 None:intergenic 50.0%
TCATGGGAGATGGACAACTG+AGG + chr7.3:19726081-19726100 MS.gene054833:CDS 50.0%
TCTGACAGGTACTCACCTCA+TGG + chr7.3:19726064-19726083 MS.gene054833:intron 50.0%
!! GTTAGTTTCGGTGTCGTGTC+TGG - chr7.3:19725839-19725858 None:intergenic 50.0%
GGTACTCACCTCATGGGAGA+TGG + chr7.3:19726071-19726090 MS.gene054833:intron 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 19725382 19726898 19725382 ID=MS.gene054833
chr7.3 mRNA 19725382 19726898 19725382 ID=MS.gene054833.t1;Parent=MS.gene054833
chr7.3 exon 19725382 19725482 19725382 ID=MS.gene054833.t1.exon1;Parent=MS.gene054833.t1
chr7.3 CDS 19725382 19725482 19725382 ID=cds.MS.gene054833.t1;Parent=MS.gene054833.t1
chr7.3 exon 19725628 19725707 19725628 ID=MS.gene054833.t1.exon2;Parent=MS.gene054833.t1
chr7.3 CDS 19725628 19725707 19725628 ID=cds.MS.gene054833.t1;Parent=MS.gene054833.t1
chr7.3 exon 19726072 19726244 19726072 ID=MS.gene054833.t1.exon3;Parent=MS.gene054833.t1
chr7.3 CDS 19726072 19726244 19726072 ID=cds.MS.gene054833.t1;Parent=MS.gene054833.t1
chr7.3 exon 19726528 19726602 19726528 ID=MS.gene054833.t1.exon4;Parent=MS.gene054833.t1
chr7.3 CDS 19726528 19726602 19726528 ID=cds.MS.gene054833.t1;Parent=MS.gene054833.t1
chr7.3 exon 19726711 19726898 19726711 ID=MS.gene054833.t1.exon5;Parent=MS.gene054833.t1
chr7.3 CDS 19726711 19726898 19726711 ID=cds.MS.gene054833.t1;Parent=MS.gene054833.t1
Gene Sequence

>MS.gene054833

AAAAACACATAAAGTCAAGGCCAGATGACTCAATTGCTGCTCTTTATCTTTCTCTTGAGAATGGAAATTTTTGGTTCCCTGCCCAAGTTTATAACCGTGAGGTAAATACTGGTTGCATGCAGATTTTGGTAGTAATTATTTTCATAATTGATCCCTGATTTAAAAATTAGTATTAATTAATATTTTGTCTTGTAATTAAGGGATTTTGTTAAGTATAATTGTTTGACATTTTAAGTACATTTTCAGAATGGTCATGCTGGATTCATGCTCTCATGTTATGATGCTGAACTTTGCTATGACTCTAGAACAGATGCCTTTCAAGCAAGGTATGTGCAAAAGTTGACTCATTCAATATTGTTGAGTTTAGGTGCCAAACAACTTGTCTTTTTACCTGTCCTATTGTACTTTTGGTTTAATTCTGAAGTTATAATAACCTATGAAATACTAAAGGATACCAGACACGACACCGAAACTAACACGTAGACACTAATAATAATTTAAGAAAATGAAAATAATTGAATATAACCACATGTGTTAGTGTCATGTGCCAGTGTCAGACACCAGAATGATTTTGGTCTGAAGTGTCGTGCTACATAGTTTATAACTAAACTTTCAATAGTTTAACATTTTATAGGAATTTTTTTTTTTTCAAACTCTAAACATGAAGTTCATTCATGTTTTTTCTGACAGGTACTCACCTCATGGGAGATGGACAACTGAGGAAAACATAAAATGGGAAAGGCTAAGAGTGCCACCTATTGACTCTTCTTCACATGTTCTTCATATCTCTGACTGCTTAGATGACTTAAGGCCTGGAGATCATGTTGAAATTCAGTGGAGAAGAAACAAGGAGTTCCCTTATGGTATGTCTCATCCTAGTTGTTGTGCAATATCTAAAAACTCATGATTGCTACATGTAACCTCTTCTAAATAGTCGCATAAATGCTTATGTCAGTAGATAAATAAAGGTCGGTCTTGACAGACCTAGAATATTAACTATTATCAATGTTTGTGATAGATTGGACTGATATATATATTATATAACTCATTCTTAAACTTTTTGTCAAGAACAAATATTAATATCAATTTTGTCATGATATGCTTGATTATCATCATGCACTAATTTTTAAAAATGTTTTGTTATAGGTTGGTGGTATAGTGTCATTGGTCATTTGGAAACATGTCAAGGTGATGGGAACCATTGCCAATGTCACAATAAAGGTAAGAATATCCACTAGCATACATTTTAATTATTTTGTTTTTCGCCCCTTAAATTATGTTGTCACTTTATTGTATCTATGATGATTAAATCAATAATGTTGGTGACAGATCCAGTGATTTTGGAGTTCACACAATACACAGCTGGCTCAAGATGGAGACAAACAATGATAAACAGAAAAAATCATAGAGAACAAGGAAATGAAATAGAAGGTTTTTATGGGGGAATCAGAAAGTTGCATAGCAAGGAGGAGATTACAAAGTGGAAGAAACTTTGGCCAACAAAAAATGTTGAATAG

Protein sequence

>MS.gene054833.t1

KNTUSQGQMTQLLLFIFLLRMEIFGSLPKFITVRMVMLDSCSHVMMLNFAMTLEQMPFKQGTHLMGDGQLRKTUNGKGUECHLLTLLHMFFISLTAUMTUGLEIMLKFSGEETRSSLMVGGIVSLVIWKHVKVMGTIANVTIKIQUFWSSHNTQLAQDGDKQUUTEKIIENKEMKUKVFMGESESCIARRRLQSGRNFGQQKMLN