AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51957


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51957.t1 MTR_3g010290 98.201 278 5 0 1 278 1 278 0.0 566
MS.gene51957.t1 MTR_3g091610 26.374 273 143 8 8 277 13 230 2.07e-20 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51957.t1 AT4G10170 54.122 279 101 5 1 278 1 253 1.16e-93 277
MS.gene51957.t1 AT4G10170 54.122 279 101 5 1 278 1 253 1.16e-93 277
MS.gene51957.t1 AT1G33475 44.326 282 125 7 1 278 1 254 4.10e-71 219
MS.gene51957.t1 AT1G33475 44.954 109 48 2 171 278 55 152 9.88e-24 94.4
MS.gene51957.t1 AT1G33475 57.143 56 22 1 1 54 1 56 2.25e-16 74.7
MS.gene51957.t1 AT4G27840 25.175 286 177 7 1 277 1 258 4.76e-19 84.7
MS.gene51957.t1 AT5G52990 24.823 282 179 9 10 278 10 271 1.43e-15 75.1

Find 60 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCTTTGCAAAAGGATA+TGG 0.148205 3.2:-4057040 MS.gene51957:CDS
AAGGCCTGATTCAGGAAATT+TGG 0.252155 3.2:-4057094 MS.gene51957:CDS
TTCAACCAAAGCCCCTTTAT+TGG 0.253713 3.2:-4057217 MS.gene51957:CDS
TTTCACATAGTGGTAGTAAT+TGG 0.275380 3.2:-4057320 MS.gene51957:CDS
AATGAGAGATGTTGAGTTAG+AGG 0.307965 3.2:-4057145 MS.gene51957:CDS
TCAACCAAAGCCCCTTTATT+GGG 0.328565 3.2:-4057216 MS.gene51957:CDS
GTATAATATTATTTGTCATC+TGG 0.366131 3.2:-4056927 MS.gene51957:CDS
GACGAAACTTCCTCGACATT+TGG 0.372647 3.2:-4057294 MS.gene51957:CDS
ATTTCGAGACAATAGGTAAA+AGG 0.375196 3.2:-4057563 MS.gene51957:CDS
CCGTCCAAATTTCCTGAATC+AGG 0.383998 3.2:+4057090 None:intergenic
AGTTTACGTTCAAGAGAAAC+TGG 0.388560 3.2:-4057382 MS.gene51957:CDS
AGAGGATCAAGGCCTGATTC+AGG 0.392539 3.2:-4057102 MS.gene51957:CDS
ATTTGTCCATTCAAATTACT+TGG 0.402162 3.2:+4057252 None:intergenic
TACATCTTCTTCTTTGCAAA+AGG 0.409637 3.2:-4057046 MS.gene51957:CDS
AGATGAAGGTCTTGGTAACT+TGG 0.412330 3.2:-4057493 MS.gene51957:CDS
TCTTCTTTGCAAAAGGATAT+GGG 0.415200 3.2:-4057039 MS.gene51957:CDS
TAGCTAGAACAATGCGTACT+TGG 0.417134 3.2:+4056965 None:intergenic
CTCAAAACGTCCGTAAGAAA+TGG 0.447780 3.2:-4056993 MS.gene51957:CDS
TACTTCACAATTGTAGATGA+AGG 0.455781 3.2:-4057507 MS.gene51957:CDS
CATTACTGTTGTGTTTCTAA+AGG 0.456727 3.2:-4057681 MS.gene51957:CDS
CCATTCAAATTACTTGGTGA+TGG 0.462379 3.2:+4057258 None:intergenic
CACAGCCTTGTGCTTGGAGG+CGG 0.462743 3.2:-4057607 MS.gene51957:CDS
ACGTAAACTGAATTCATGTT+TGG 0.465378 3.2:+4057396 None:intergenic
ACAATTGTAGATGAAGGTCT+TGG 0.466801 3.2:-4057501 MS.gene51957:CDS
ACAATAGGTAAAAGGACTTA+CGG 0.467377 3.2:-4057555 MS.gene51957:CDS
CTTGGCGCCACCATTTCTTA+CGG 0.469508 3.2:+4056983 None:intergenic
CAATAGGTAAAAGGACTTAC+GGG 0.479243 3.2:-4057554 MS.gene51957:CDS
TTAGAGAATGTTTCACATAG+TGG 0.480167 3.2:-4057330 MS.gene51957:CDS
AGGCGGTTCCTTCTTTCCAC+AGG 0.481926 3.2:-4057590 MS.gene51957:CDS
CACATCTTGTATGCGTATAG+TGG 0.484986 3.2:-4057654 MS.gene51957:CDS
AAATTACTTGGTGATGGAGA+TGG 0.492575 3.2:+4057264 None:intergenic
TTCAGAAAGGTAGCTAGAAA+AGG 0.498079 3.2:-4057435 MS.gene51957:CDS
AGGTGGTATTTCGAGACAAT+AGG 0.503747 3.2:-4057570 MS.gene51957:CDS
GGTTCAATGAGGACGAGATC+AGG 0.506463 3.2:-4057018 MS.gene51957:CDS
AGGTAGCTAGAAAAGGTTCA+AGG 0.518283 3.2:-4057428 MS.gene51957:CDS
CGGTTCCTTCTTTCCACAGG+TGG 0.523709 3.2:-4057587 MS.gene51957:CDS
CCATCACCAAGTAATTTGAA+TGG 0.529421 3.2:-4057258 MS.gene51957:CDS
AAATACCACCTGTGGAAAGA+AGG 0.529530 3.2:+4057582 None:intergenic
GTGATCAGATTACGAATGAC+AGG 0.539134 3.2:+4057357 None:intergenic
ACTTCCTCGACATTTGGCAT+TGG 0.540398 3.2:-4057288 MS.gene51957:CDS
GCAAGAGAAGAAGAAAGTAA+AGG 0.545957 3.2:-4057181 MS.gene51957:CDS
TGTCACAGCCTTGTGCTTGG+AGG 0.553859 3.2:-4057610 MS.gene51957:CDS
GAATGTCACAGCCTTGTGCT+TGG 0.555477 3.2:-4057613 MS.gene51957:CDS
AATCTACAGATAGAGGATCA+AGG 0.570567 3.2:-4057113 MS.gene51957:CDS
GGTAGCTAGAAAAGGTTCAA+GGG 0.580926 3.2:-4057427 MS.gene51957:CDS
ATCTTGTATGCGTATAGTGG+TGG 0.589645 3.2:-4057651 MS.gene51957:CDS
AAAACGTCCGTAAGAAATGG+TGG 0.598377 3.2:-4056990 MS.gene51957:CDS
CACTATACGCATACAAGATG+TGG 0.605232 3.2:+4057655 None:intergenic
TTGTCTCGAAATACCACCTG+TGG 0.605879 3.2:+4057574 None:intergenic
AGGAACCGCCTCCAAGCACA+AGG 0.610638 3.2:+4057602 None:intergenic
GTAGCTAGAAAAGGTTCAAG+GGG 0.613162 3.2:-4057426 MS.gene51957:CDS
AAGATGTAGCAGCACCACCT+TGG 0.620183 3.2:+4057061 None:intergenic
AATTTGGACGGTATTAGCCA+AGG 0.623716 3.2:-4057078 MS.gene51957:CDS
TGTCAGAGATGAATTCAGAA+AGG 0.624051 3.2:-4057448 MS.gene51957:CDS
CCTGATTCAGGAAATTTGGA+CGG 0.627032 3.2:-4057090 MS.gene51957:CDS
ATCGAATGCATGATATACCA+CGG 0.650850 3.2:+4056893 None:intergenic
ATCTGGTTAGTAATATGCCG+TGG 0.655337 3.2:-4056910 MS.gene51957:CDS
TGGACCAATGCCAAATGTCG+AGG 0.656618 3.2:+4057284 None:intergenic
TTGGACGGTATTAGCCAAGG+TGG 0.682644 3.2:-4057075 MS.gene51957:CDS
AAAAGGATATGGGTTCAATG+AGG 0.721400 3.2:-4057029 MS.gene51957:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATAATATTATTTGTCATC+TGG - chr3.2:4057669-4057688 MS.gene51957:CDS 20.0%
! ATTTGTCCATTCAAATTACT+TGG + chr3.2:4057347-4057366 None:intergenic 25.0%
! CATAAAAAATCTACAGATAG+AGG - chr3.2:4057476-4057495 MS.gene51957:CDS 25.0%
ACAATAGGTAAAAGGACTTA+CGG - chr3.2:4057041-4057060 MS.gene51957:CDS 30.0%
ACGTAAACTGAATTCATGTT+TGG + chr3.2:4057203-4057222 None:intergenic 30.0%
ATTTCGAGACAATAGGTAAA+AGG - chr3.2:4057033-4057052 MS.gene51957:CDS 30.0%
TACATCTTCTTCTTTGCAAA+AGG - chr3.2:4057550-4057569 MS.gene51957:CDS 30.0%
TACTTCACAATTGTAGATGA+AGG - chr3.2:4057089-4057108 MS.gene51957:CDS 30.0%
TCTTCTTTGCAAAAGGATAT+GGG - chr3.2:4057557-4057576 MS.gene51957:CDS 30.0%
TTAGAGAATGTTTCACATAG+TGG - chr3.2:4057266-4057285 MS.gene51957:CDS 30.0%
TTCTTCTTTGCAAAAGGATA+TGG - chr3.2:4057556-4057575 MS.gene51957:CDS 30.0%
TTTCACATAGTGGTAGTAAT+TGG - chr3.2:4057276-4057295 MS.gene51957:CDS 30.0%
! CATTACTGTTGTGTTTCTAA+AGG - chr3.2:4056915-4056934 MS.gene51957:CDS 30.0%
! TGCTAGATTTTCCCAATAAA+GGG + chr3.2:4057394-4057413 None:intergenic 30.0%
! TTGCTAGATTTTCCCAATAA+AGG + chr3.2:4057395-4057414 None:intergenic 30.0%
AAAAGGATATGGGTTCAATG+AGG - chr3.2:4057567-4057586 MS.gene51957:CDS 35.0%
AATCTACAGATAGAGGATCA+AGG - chr3.2:4057483-4057502 MS.gene51957:CDS 35.0%
AATGAGAGATGTTGAGTTAG+AGG - chr3.2:4057451-4057470 MS.gene51957:CDS 35.0%
ACAATTGTAGATGAAGGTCT+TGG - chr3.2:4057095-4057114 MS.gene51957:CDS 35.0%
AGTTTACGTTCAAGAGAAAC+TGG - chr3.2:4057214-4057233 MS.gene51957:CDS 35.0%
ATCGAATGCATGATATACCA+CGG + chr3.2:4057706-4057725 None:intergenic 35.0%
CAATAGGTAAAAGGACTTAC+GGG - chr3.2:4057042-4057061 MS.gene51957:CDS 35.0%
CCATCACCAAGTAATTTGAA+TGG - chr3.2:4057338-4057357 MS.gene51957:CDS 35.0%
GCAAGAGAAGAAGAAAGTAA+AGG - chr3.2:4057415-4057434 MS.gene51957:CDS 35.0%
TGTCAGAGATGAATTCAGAA+AGG - chr3.2:4057148-4057167 MS.gene51957:CDS 35.0%
TTCAGAAAGGTAGCTAGAAA+AGG - chr3.2:4057161-4057180 MS.gene51957:CDS 35.0%
! ATTTCTTACGGACGTTTTGA+GGG + chr3.2:4057604-4057623 None:intergenic 35.0%
! CCATTCAAATTACTTGGTGA+TGG + chr3.2:4057341-4057360 None:intergenic 35.0%
! GCTAGATTTTCCCAATAAAG+GGG + chr3.2:4057393-4057412 None:intergenic 35.0%
!! AAATTACTTGGTGATGGAGA+TGG + chr3.2:4057335-4057354 None:intergenic 35.0%
!! CTTGGTAGTTCTTCAGTTTT+TGG - chr3.2:4057121-4057140 MS.gene51957:CDS 35.0%
AAAACGTCCGTAAGAAATGG+TGG - chr3.2:4057606-4057625 MS.gene51957:CDS 40.0%
AAATACCACCTGTGGAAAGA+AGG + chr3.2:4057017-4057036 None:intergenic 40.0%
AAGGCCTGATTCAGGAAATT+TGG - chr3.2:4057502-4057521 MS.gene51957:CDS 40.0%
AATTTGGACGGTATTAGCCA+AGG - chr3.2:4057518-4057537 MS.gene51957:CDS 40.0%
AGGTAGCTAGAAAAGGTTCA+AGG - chr3.2:4057168-4057187 MS.gene51957:CDS 40.0%
AGGTGGTATTTCGAGACAAT+AGG - chr3.2:4057026-4057045 MS.gene51957:CDS 40.0%
ATCTGGTTAGTAATATGCCG+TGG - chr3.2:4057686-4057705 MS.gene51957:CDS 40.0%
ATCTTGTATGCGTATAGTGG+TGG - chr3.2:4056945-4056964 MS.gene51957:CDS 40.0%
CACATCTTGTATGCGTATAG+TGG - chr3.2:4056942-4056961 MS.gene51957:CDS 40.0%
CACTATACGCATACAAGATG+TGG + chr3.2:4056944-4056963 None:intergenic 40.0%
CCTGATTCAGGAAATTTGGA+CGG - chr3.2:4057506-4057525 MS.gene51957:CDS 40.0%
CTCAAAACGTCCGTAAGAAA+TGG - chr3.2:4057603-4057622 MS.gene51957:CDS 40.0%
GGTAGCTAGAAAAGGTTCAA+GGG - chr3.2:4057169-4057188 MS.gene51957:CDS 40.0%
GTAGCTAGAAAAGGTTCAAG+GGG - chr3.2:4057170-4057189 MS.gene51957:CDS 40.0%
GTGATCAGATTACGAATGAC+AGG + chr3.2:4057242-4057261 None:intergenic 40.0%
TAGCTAGAACAATGCGTACT+TGG + chr3.2:4057634-4057653 None:intergenic 40.0%
TCAACCAAAGCCCCTTTATT+GGG - chr3.2:4057380-4057399 MS.gene51957:CDS 40.0%
TTCAACCAAAGCCCCTTTAT+TGG - chr3.2:4057379-4057398 MS.gene51957:CDS 40.0%
! AGATGAAGGTCTTGGTAACT+TGG - chr3.2:4057103-4057122 MS.gene51957:CDS 40.0%
! CATTTCTTACGGACGTTTTG+AGG + chr3.2:4057605-4057624 None:intergenic 40.0%
! TTTTCCCAATAAAGGGGCTT+TGG + chr3.2:4057387-4057406 None:intergenic 40.0%
!! AAGGACTTACGGGTTTTTAG+TGG - chr3.2:4057052-4057071 MS.gene51957:CDS 40.0%
!!! TACGGGTTTTTAGTGGAAGA+TGG - chr3.2:4057059-4057078 MS.gene51957:CDS 40.0%
ACTTCCTCGACATTTGGCAT+TGG - chr3.2:4057308-4057327 MS.gene51957:CDS 45.0%
CCGTCCAAATTTCCTGAATC+AGG + chr3.2:4057509-4057528 None:intergenic 45.0%
GACGAAACTTCCTCGACATT+TGG - chr3.2:4057302-4057321 MS.gene51957:CDS 45.0%
TTGTCTCGAAATACCACCTG+TGG + chr3.2:4057025-4057044 None:intergenic 45.0%
AAGATGTAGCAGCACCACCT+TGG + chr3.2:4057538-4057557 None:intergenic 50.0%
AGAGGATCAAGGCCTGATTC+AGG - chr3.2:4057494-4057513 MS.gene51957:CDS 50.0%
CTTGGCGCCACCATTTCTTA+CGG + chr3.2:4057616-4057635 None:intergenic 50.0%
GGTTCAATGAGGACGAGATC+AGG - chr3.2:4057578-4057597 MS.gene51957:CDS 50.0%
TGGACCAATGCCAAATGTCG+AGG + chr3.2:4057315-4057334 None:intergenic 50.0%
TTGGACGGTATTAGCCAAGG+TGG - chr3.2:4057521-4057540 MS.gene51957:CDS 50.0%
! GAATGTCACAGCCTTGTGCT+TGG - chr3.2:4056983-4057002 MS.gene51957:CDS 50.0%
AGGCGGTTCCTTCTTTCCAC+AGG - chr3.2:4057006-4057025 MS.gene51957:CDS 55.0%
CGGTTCCTTCTTTCCACAGG+TGG - chr3.2:4057009-4057028 MS.gene51957:CDS 55.0%
! TGTCACAGCCTTGTGCTTGG+AGG - chr3.2:4056986-4057005 MS.gene51957:CDS 55.0%
AGGAACCGCCTCCAAGCACA+AGG + chr3.2:4056997-4057016 None:intergenic 60.0%
! CACAGCCTTGTGCTTGGAGG+CGG - chr3.2:4056989-4057008 MS.gene51957:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 4056891 4057727 4056891 ID=MS.gene51957
chr3.2 mRNA 4056891 4057727 4056891 ID=MS.gene51957.t1;Parent=MS.gene51957
chr3.2 exon 4056891 4057727 4056891 ID=MS.gene51957.t1.exon1;Parent=MS.gene51957.t1
chr3.2 CDS 4056891 4057727 4056891 ID=cds.MS.gene51957.t1;Parent=MS.gene51957.t1
Gene Sequence

>MS.gene51957

ATGGATTCAATTCAAAATAAAGTTCATTACTGTTGTGTTTCTAAAGGTAGCCACATCTTGTATGCGTATAGTGGTGGAGATAAAGAAGTTGAGAATGTCACAGCCTTGTGCTTGGAGGCGGTTCCTTCTTTCCACAGGTGGTATTTCGAGACAATAGGTAAAAGGACTTACGGGTTTTTAGTGGAAGATGGATATGTTTACTTCACAATTGTAGATGAAGGTCTTGGTAACTTGGTAGTTCTTCAGTTTTTGGAACATGTCAGAGATGAATTCAGAAAGGTAGCTAGAAAAGGTTCAAGGGGATTTTCACCAAACATGAATTCAGTTTACGTTCAAGAGAAACTGGTTCCTGTCATTCGTAATCTGATCACTTCATTAGAGAATGTTTCACATAGTGGTAGTAATTGGAGAGACGAAACTTCCTCGACATTTGGCATTGGTCCATCTCCATCACCAAGTAATTTGAATGGACAAATTGAAAATGTTGTTTCAACCAAAGCCCCTTTATTGGGAAAATCTAGCAAGCAAGAGAAGAAGAAAGTAAAGGATCATGTGATTGCAATGAGAGATGTTGAGTTAGAGGAGCATAAAAAATCTACAGATAGAGGATCAAGGCCTGATTCAGGAAATTTGGACGGTATTAGCCAAGGTGGTGCTGCTACATCTTCTTCTTTGCAAAAGGATATGGGTTCAATGAGGACGAGATCAGGCCCTCAAAACGTCCGTAAGAAATGGTGGCGCCAAGTACGCATTGTTCTAGCTATCGATGCAGCTGTATGTATAATATTATTTGTCATCTGGTTAGTAATATGCCGTGGTATATCATGCATTCGATAG

Protein sequence

>MS.gene51957.t1

MDSIQNKVHYCCVSKGSHILYAYSGGDKEVENVTALCLEAVPSFHRWYFETIGKRTYGFLVEDGYVYFTIVDEGLGNLVVLQFLEHVRDEFRKVARKGSRGFSPNMNSVYVQEKLVPVIRNLITSLENVSHSGSNWRDETSSTFGIGPSPSPSNLNGQIENVVSTKAPLLGKSSKQEKKKVKDHVIAMRDVELEEHKKSTDRGSRPDSGNLDGISQGGAATSSSLQKDMGSMRTRSGPQNVRKKWWRQVRIVLAIDAAVCIILFVIWLVICRGISCIR