Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54530.t1 | RHN44125.1 | 98.4 | 498 | 8 | 0 | 1 | 498 | 1 | 498 | 3.10E-256 | 894 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54530.t1 | Q9FLD5 | 62.3 | 467 | 163 | 5 | 1 | 460 | 4 | 464 | 2.5e-153 | 543.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54530.t1 | A0A396GZ58 | 98.4 | 498 | 8 | 0 | 1 | 498 | 1 | 498 | 2.2e-256 | 894.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049850 | MS.gene54530 | 0.813964 | 1.96E-51 | -1.69E-46 |
| MS.gene050020 | MS.gene54530 | 0.864525 | 1.14E-64 | -1.69E-46 |
| MS.gene05016 | MS.gene54530 | 0.809269 | 2.06E-50 | -1.69E-46 |
| MS.gene050273 | MS.gene54530 | 0.808833 | 2.55E-50 | -1.69E-46 |
| MS.gene050585 | MS.gene54530 | 0.80909 | 2.25E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene54530 | 0.870533 | 1.36E-66 | -1.69E-46 |
| MS.gene050808 | MS.gene54530 | 0.843419 | 1.42E-58 | -1.69E-46 |
| MS.gene05157 | MS.gene54530 | 0.84703 | 1.50E-59 | -1.69E-46 |
| MS.gene051719 | MS.gene54530 | 0.864349 | 1.30E-64 | -1.69E-46 |
| MS.gene051720 | MS.gene54530 | 0.850633 | 1.50E-60 | -1.69E-46 |
| MS.gene052133 | MS.gene54530 | 0.802169 | 6.37E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene54530 | 0.825803 | 3.84E-54 | -1.69E-46 |
| MS.gene052665 | MS.gene54530 | 0.850877 | 1.28E-60 | -1.69E-46 |
| MS.gene052732 | MS.gene54530 | 0.801192 | 1.01E-48 | -1.69E-46 |
| MS.gene053144 | MS.gene54530 | 0.832942 | 7.09E-56 | -1.69E-46 |
| MS.gene053174 | MS.gene54530 | 0.809377 | 1.95E-50 | -1.69E-46 |
| MS.gene053563 | MS.gene54530 | 0.815643 | 8.32E-52 | -1.69E-46 |
| MS.gene053581 | MS.gene54530 | 0.853722 | 1.98E-61 | -1.69E-46 |
| MS.gene053894 | MS.gene54530 | 0.805095 | 1.58E-49 | -1.69E-46 |
| MS.gene054550 | MS.gene54530 | 0.807864 | 4.10E-50 | -1.69E-46 |
| MS.gene054584 | MS.gene54530 | 0.83062 | 2.65E-55 | -1.69E-46 |
| MS.gene054791 | MS.gene54530 | 0.848015 | 8.02E-60 | -1.69E-46 |
| MS.gene055144 | MS.gene54530 | 0.813428 | 2.57E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene54530 | 0.840801 | 6.99E-58 | -1.69E-46 |
| MS.gene055466 | MS.gene54530 | 0.838055 | 3.61E-57 | -1.69E-46 |
| MS.gene055467 | MS.gene54530 | 0.809193 | 2.14E-50 | -1.69E-46 |
| MS.gene055468 | MS.gene54530 | 0.829375 | 5.33E-55 | -1.69E-46 |
| MS.gene055469 | MS.gene54530 | 0.807152 | 5.81E-50 | -1.69E-46 |
| MS.gene055512 | MS.gene54530 | 0.815741 | 7.91E-52 | -1.69E-46 |
| MS.gene055580 | MS.gene54530 | -0.807604 | 4.66E-50 | -1.69E-46 |
| MS.gene055890 | MS.gene54530 | 0.838582 | 2.64E-57 | -1.69E-46 |
| MS.gene055891 | MS.gene54530 | 0.833691 | 4.61E-56 | -1.69E-46 |
| MS.gene056005 | MS.gene54530 | 0.829763 | 4.29E-55 | -1.69E-46 |
| MS.gene056308 | MS.gene54530 | 0.80362 | 3.20E-49 | -1.69E-46 |
| MS.gene056553 | MS.gene54530 | 0.821115 | 4.79E-53 | -1.69E-46 |
| MS.gene059937 | MS.gene54530 | 0.81345 | 2.54E-51 | -1.69E-46 |
| MS.gene060519 | MS.gene54530 | 0.857523 | 1.54E-62 | -1.69E-46 |
| MS.gene060587 | MS.gene54530 | 0.843748 | 1.16E-58 | -1.69E-46 |
| MS.gene061176 | MS.gene54530 | 0.83915 | 1.88E-57 | -1.69E-46 |
| MS.gene061177 | MS.gene54530 | 0.838496 | 2.78E-57 | -1.69E-46 |
| MS.gene061178 | MS.gene54530 | 0.838815 | 2.30E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54530.t1 | MTR_7g008040 | 97.984 | 496 | 10 | 0 | 1 | 496 | 1 | 496 | 0.0 | 1001 |
| MS.gene54530.t1 | MTR_6g009540 | 66.523 | 463 | 146 | 4 | 1 | 460 | 3 | 459 | 0.0 | 630 |
| MS.gene54530.t1 | MTR_6g009520 | 67.171 | 463 | 143 | 4 | 1 | 460 | 5 | 461 | 0.0 | 627 |
| MS.gene54530.t1 | MTR_4g107370 | 58.893 | 506 | 196 | 5 | 1 | 495 | 6 | 510 | 0.0 | 603 |
| MS.gene54530.t1 | MTR_1g022250 | 61.396 | 487 | 162 | 8 | 2 | 485 | 4 | 467 | 0.0 | 591 |
| MS.gene54530.t1 | MTR_6g009720 | 63.034 | 468 | 160 | 4 | 1 | 459 | 5 | 468 | 0.0 | 583 |
| MS.gene54530.t1 | MTR_2g030420 | 59.414 | 478 | 173 | 6 | 2 | 477 | 7 | 465 | 0.0 | 577 |
| MS.gene54530.t1 | MTR_6g009553 | 63.556 | 450 | 148 | 5 | 23 | 460 | 3 | 448 | 0.0 | 565 |
| MS.gene54530.t1 | MTR_6g009770 | 61.064 | 470 | 146 | 5 | 1 | 460 | 5 | 447 | 0.0 | 553 |
| MS.gene54530.t1 | MTR_6g042290 | 60.659 | 455 | 153 | 6 | 8 | 460 | 21 | 451 | 0.0 | 542 |
| MS.gene54530.t1 | MTR_6g009510 | 53.707 | 499 | 146 | 7 | 7 | 489 | 20 | 449 | 1.15e-174 | 499 |
| MS.gene54530.t1 | MTR_1g022245 | 52.371 | 464 | 212 | 4 | 1 | 459 | 1 | 460 | 6.84e-174 | 499 |
| MS.gene54530.t1 | MTR_6g009660 | 54.310 | 464 | 148 | 10 | 4 | 460 | 5 | 411 | 1.46e-157 | 456 |
| MS.gene54530.t1 | MTR_4g128260 | 42.857 | 455 | 231 | 10 | 1 | 440 | 1 | 441 | 1.03e-118 | 358 |
| MS.gene54530.t1 | MTR_5g020980 | 39.959 | 483 | 243 | 10 | 2 | 472 | 11 | 458 | 7.12e-117 | 353 |
| MS.gene54530.t1 | MTR_2g061820 | 38.641 | 471 | 256 | 9 | 1 | 464 | 4 | 448 | 1.33e-110 | 338 |
| MS.gene54530.t1 | MTR_2g076710 | 38.723 | 470 | 246 | 8 | 10 | 471 | 14 | 449 | 2.09e-110 | 335 |
| MS.gene54530.t1 | MTR_2g076920 | 38.922 | 501 | 238 | 11 | 8 | 487 | 13 | 466 | 3.45e-110 | 335 |
| MS.gene54530.t1 | MTR_2g076690 | 40.471 | 467 | 231 | 10 | 10 | 468 | 16 | 443 | 9.84e-109 | 331 |
| MS.gene54530.t1 | MTR_2g076970 | 38.809 | 487 | 247 | 11 | 8 | 482 | 7 | 454 | 1.12e-108 | 331 |
| MS.gene54530.t1 | MTR_2g076840 | 39.875 | 479 | 237 | 11 | 8 | 477 | 7 | 443 | 4.97e-106 | 332 |
| MS.gene54530.t1 | MTR_2g076840 | 50.190 | 263 | 105 | 3 | 198 | 460 | 467 | 703 | 7.91e-80 | 263 |
| MS.gene54530.t1 | MTR_8g076920 | 37.778 | 495 | 261 | 8 | 9 | 487 | 28 | 491 | 1.04e-105 | 325 |
| MS.gene54530.t1 | MTR_2g076900 | 38.289 | 491 | 247 | 12 | 8 | 487 | 7 | 452 | 2.68e-103 | 317 |
| MS.gene54530.t1 | MTR_2g076990 | 40.143 | 421 | 209 | 8 | 61 | 473 | 1 | 386 | 1.44e-99 | 307 |
| MS.gene54530.t1 | MTR_5g020990 | 36.420 | 486 | 263 | 9 | 5 | 472 | 25 | 482 | 1.18e-93 | 293 |
| MS.gene54530.t1 | MTR_3g103150 | 42.997 | 307 | 139 | 5 | 156 | 460 | 4 | 276 | 8.02e-79 | 248 |
| MS.gene54530.t1 | MTR_7g117950 | 34.324 | 370 | 183 | 10 | 93 | 460 | 94 | 405 | 2.25e-63 | 213 |
| MS.gene54530.t1 | MTR_3g092060 | 31.989 | 372 | 195 | 8 | 95 | 460 | 95 | 414 | 1.48e-59 | 204 |
| MS.gene54530.t1 | MTR_8g027200 | 28.358 | 469 | 240 | 13 | 39 | 485 | 38 | 432 | 1.84e-51 | 181 |
| MS.gene54530.t1 | MTR_5g021020 | 35.604 | 323 | 154 | 8 | 140 | 460 | 135 | 405 | 2.49e-51 | 180 |
| MS.gene54530.t1 | MTR_1g011680 | 63.235 | 136 | 47 | 1 | 324 | 459 | 1 | 133 | 4.03e-50 | 169 |
| MS.gene54530.t1 | MTR_5g021000 | 50.926 | 108 | 48 | 2 | 355 | 460 | 1 | 105 | 8.33e-30 | 115 |
| MS.gene54530.t1 | MTR_5g020720 | 36.364 | 187 | 92 | 5 | 262 | 446 | 25 | 186 | 5.92e-27 | 107 |
| MS.gene54530.t1 | MTR_1g113750 | 54.455 | 101 | 43 | 2 | 388 | 485 | 16 | 116 | 1.57e-24 | 99.0 |
| MS.gene54530.t1 | MTR_5g021010 | 47.826 | 92 | 43 | 2 | 372 | 461 | 34 | 122 | 7.02e-18 | 80.1 |
| MS.gene54530.t1 | MTR_2g076820 | 55.769 | 52 | 23 | 0 | 388 | 439 | 1 | 52 | 5.66e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54530.t1 | AT5G40010 | 61.987 | 463 | 163 | 6 | 1 | 456 | 4 | 460 | 0.0 | 577 |
| MS.gene54530.t1 | AT3G28580 | 60.870 | 460 | 168 | 6 | 1 | 456 | 4 | 455 | 0.0 | 558 |
| MS.gene54530.t1 | AT3G28580 | 57.174 | 460 | 163 | 7 | 1 | 456 | 4 | 433 | 0.0 | 516 |
| MS.gene54530.t1 | AT3G28510 | 55.292 | 463 | 196 | 5 | 4 | 463 | 7 | 461 | 1.40e-179 | 514 |
| MS.gene54530.t1 | AT3G28610 | 52.556 | 489 | 191 | 10 | 8 | 481 | 6 | 468 | 1.30e-171 | 492 |
| MS.gene54530.t1 | AT3G28600 | 52.282 | 482 | 188 | 8 | 8 | 474 | 7 | 461 | 7.87e-170 | 488 |
| MS.gene54530.t1 | AT3G28540 | 53.947 | 456 | 202 | 4 | 4 | 458 | 7 | 455 | 2.84e-169 | 488 |
| MS.gene54530.t1 | AT3G28540 | 53.947 | 456 | 202 | 4 | 4 | 458 | 7 | 455 | 3.54e-169 | 487 |
| MS.gene54530.t1 | AT5G40000 | 49.491 | 491 | 200 | 12 | 1 | 474 | 4 | 463 | 2.74e-158 | 458 |
| MS.gene54530.t1 | AT3G28520 | 50.932 | 483 | 209 | 6 | 12 | 477 | 1 | 472 | 8.32e-154 | 447 |
| MS.gene54530.t1 | AT3G28600 | 48.133 | 482 | 169 | 7 | 8 | 474 | 7 | 422 | 7.33e-148 | 430 |
| MS.gene54530.t1 | AT3G28570 | 46.708 | 486 | 199 | 9 | 6 | 472 | 14 | 458 | 6.90e-145 | 423 |
| MS.gene54530.t1 | AT3G28570 | 46.708 | 486 | 199 | 9 | 6 | 472 | 7 | 451 | 7.49e-145 | 423 |
| MS.gene54530.t1 | AT5G57480 | 38.446 | 489 | 268 | 10 | 1 | 473 | 1 | 472 | 2.22e-115 | 350 |
| MS.gene54530.t1 | AT4G25835 | 39.030 | 474 | 268 | 8 | 1 | 468 | 1 | 459 | 1.73e-114 | 347 |
| MS.gene54530.t1 | AT3G50930 | 39.836 | 487 | 250 | 9 | 16 | 485 | 73 | 533 | 2.31e-112 | 344 |
| MS.gene54530.t1 | AT3G50940 | 38.581 | 451 | 233 | 7 | 20 | 463 | 31 | 444 | 1.45e-109 | 333 |
| MS.gene54530.t1 | AT2G18193 | 37.500 | 488 | 266 | 8 | 9 | 492 | 19 | 471 | 9.29e-107 | 327 |
| MS.gene54530.t1 | AT4G30250 | 38.382 | 482 | 268 | 9 | 1 | 468 | 1 | 467 | 2.18e-105 | 324 |
| MS.gene54530.t1 | AT4G30250 | 38.382 | 482 | 268 | 9 | 1 | 468 | 3 | 469 | 2.55e-105 | 324 |
| MS.gene54530.t1 | AT4G05380 | 38.155 | 477 | 261 | 7 | 1 | 472 | 12 | 459 | 1.72e-104 | 320 |
| MS.gene54530.t1 | AT5G17740 | 35.818 | 483 | 267 | 10 | 2 | 472 | 11 | 462 | 1.48e-98 | 307 |
| MS.gene54530.t1 | AT5G17760 | 40.376 | 426 | 198 | 9 | 64 | 468 | 72 | 462 | 1.23e-97 | 304 |
| MS.gene54530.t1 | AT2G18190 | 35.654 | 474 | 269 | 7 | 2 | 471 | 13 | 454 | 3.08e-97 | 303 |
| MS.gene54530.t1 | AT1G43910 | 38.302 | 483 | 255 | 10 | 1 | 468 | 1 | 455 | 1.15e-95 | 298 |
| MS.gene54530.t1 | AT5G17730 | 35.169 | 472 | 260 | 7 | 6 | 465 | 11 | 448 | 1.45e-94 | 295 |
| MS.gene54530.t1 | AT5G17750 | 38.859 | 368 | 182 | 7 | 71 | 426 | 55 | 391 | 3.02e-83 | 263 |
| MS.gene54530.t1 | AT2G46620 | 31.828 | 465 | 263 | 8 | 1 | 460 | 1 | 416 | 4.67e-74 | 242 |
| MS.gene54530.t1 | AT5G17760 | 39.695 | 262 | 130 | 5 | 64 | 308 | 72 | 322 | 5.21e-54 | 185 |
| MS.gene54530.t1 | AT3G29800 | 31.389 | 360 | 185 | 9 | 103 | 457 | 75 | 377 | 1.49e-49 | 176 |
| MS.gene54530.t1 | AT3G28560 | 39.908 | 218 | 111 | 5 | 4 | 213 | 7 | 212 | 2.09e-45 | 160 |
| MS.gene54530.t1 | AT4G05340 | 50.000 | 92 | 39 | 1 | 328 | 412 | 2 | 93 | 3.00e-20 | 85.9 |
Find 69 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACGCGGTTATTTACTTTA+TGG | 0.206027 | 7.4:-90765718 | MS.gene54530:CDS |
| AATAGTAAGGTAACTTTATC+TGG | 0.207637 | 7.4:-90765406 | MS.gene54530:CDS |
| GGTTCTTGTTAAGAATTATT+TGG | 0.226983 | 7.4:-90765224 | MS.gene54530:CDS |
| GACTTCAGCTTTGAGTCTTT+TGG | 0.239776 | 7.4:+90766188 | None:intergenic |
| CATGATTTGTTTGATGAAAT+TGG | 0.246773 | 7.4:-90765190 | MS.gene54530:CDS |
| TTTCATTGTGACAGTGAAAA+AGG | 0.267935 | 7.4:-90764972 | MS.gene54530:CDS |
| AAGAAATTGCAATGAGAAAT+AGG | 0.272071 | 7.4:-90765939 | MS.gene54530:CDS |
| GTCTTTCACCTGAGAATTCA+TGG | 0.273148 | 7.4:+90766274 | None:intergenic |
| GAACATTATGGTGGCTAGTA+TGG | 0.297567 | 7.4:+90766404 | None:intergenic |
| AGCTAAGTATGAGTGAACTA+TGG | 0.321937 | 7.4:-90766440 | None:intergenic |
| GAAAACAATGTGACTCCATT+TGG | 0.322099 | 7.4:+90765861 | None:intergenic |
| AACTTGATCCTGCTCTTATT+AGG | 0.329188 | 7.4:-90765303 | MS.gene54530:CDS |
| AGAATTCATGGAAAGTGATT+TGG | 0.342817 | 7.4:+90766286 | None:intergenic |
| ATAGTAGATTTACCTGTTCC+TGG | 0.352380 | 7.4:+90765691 | None:intergenic |
| GTGGAAGATGTGAAGGAAAA+TGG | 0.360536 | 7.4:-90764995 | MS.gene54530:CDS |
| TATGGCATACATGAACATTA+TGG | 0.377040 | 7.4:+90766392 | None:intergenic |
| TCTTGAATTAACCGCGGTTA+AGG | 0.395319 | 7.4:-90765626 | MS.gene54530:CDS |
| CTTTATGGTCCTCCAGGAAC+AGG | 0.413204 | 7.4:-90765703 | MS.gene54530:CDS |
| TGATGAAGAAGACTCATTCA+TGG | 0.426724 | 7.4:-90765482 | MS.gene54530:CDS |
| TAGCCATACCAACCACTACT+TGG | 0.433604 | 7.4:+90765892 | None:intergenic |
| TGGAAAGTGATTTGGATGTA+AGG | 0.435023 | 7.4:+90766294 | None:intergenic |
| AATTTCAAGGTGTTAAACTT+TGG | 0.448086 | 7.4:-90766104 | MS.gene54530:CDS |
| ATCACTTTCCATGAATTCTC+AGG | 0.451974 | 7.4:-90766282 | MS.gene54530:CDS |
| GCAAGTGTCTCAAAAGTTGC+AGG | 0.465004 | 7.4:+90765832 | None:intergenic |
| TTTGAAGTTTAGAAATGGTA+AGG | 0.467899 | 7.4:-90765770 | MS.gene54530:CDS |
| TTCAAGGTGTTAAACTTTGG+TGG | 0.468656 | 7.4:-90766101 | MS.gene54530:CDS |
| TGATGAAATTGGTGATTTGT+TGG | 0.479236 | 7.4:-90765179 | MS.gene54530:CDS |
| GAAGTCACTGATGAATTTCA+AGG | 0.482518 | 7.4:-90766117 | MS.gene54530:CDS |
| GGTCCGCATGTGGAGGGGAG+AGG | 0.484104 | 7.4:-90765357 | MS.gene54530:CDS |
| TATTTACTTTATGGTCCTCC+AGG | 0.495610 | 7.4:-90765709 | MS.gene54530:CDS |
| AATCCAAGTAGTGGTTGGTA+TGG | 0.510050 | 7.4:-90765895 | MS.gene54530:CDS |
| ATGGCTACAAACAATCCAAA+TGG | 0.513174 | 7.4:-90765876 | MS.gene54530:CDS |
| TCAAGGTGTTAAACTTTGGT+GGG | 0.519862 | 7.4:-90766100 | MS.gene54530:CDS |
| AAATTCTCAGCAACATCAGC+AGG | 0.526229 | 7.4:+90765139 | None:intergenic |
| CTTCCTGTCCTAATAAGAGC+AGG | 0.535295 | 7.4:+90765295 | None:intergenic |
| GGAAAGTGATTTGGATGTAA+GGG | 0.538609 | 7.4:+90766295 | None:intergenic |
| CAAATAATCCAAGTAGTGGT+TGG | 0.539028 | 7.4:-90765900 | MS.gene54530:CDS |
| ATCTACTATGATAGCCGCTA+TGG | 0.541351 | 7.4:-90765677 | MS.gene54530:CDS |
| AAAAGATAGTCAAAATCCAT+TGG | 0.544634 | 7.4:-90766163 | MS.gene54530:CDS |
| GATCCTGCTCTTATTAGGAC+AGG | 0.549073 | 7.4:-90765298 | MS.gene54530:CDS |
| ATCGGTAAGGCTTGGAAACG+CGG | 0.549524 | 7.4:-90765733 | MS.gene54530:CDS |
| GAATTTGGTCCGCATGTGGA+GGG | 0.552654 | 7.4:-90765363 | MS.gene54530:CDS |
| ACATCTTCATTCACAGACTT+AGG | 0.556783 | 7.4:+90765112 | None:intergenic |
| AAAAGGATATAGTTCTGTGA+TGG | 0.559391 | 7.4:-90764955 | None:intergenic |
| TCATCAATGCTAAGAACCAA+TGG | 0.561072 | 7.4:+90766147 | None:intergenic |
| ACAAGAAGTAGAAGAAGACA+AGG | 0.563981 | 7.4:-90765035 | MS.gene54530:CDS |
| TATACAAATAATCCAAGTAG+TGG | 0.565102 | 7.4:-90765904 | MS.gene54530:CDS |
| GCTAAGTATGAGTGAACTAT+GGG | 0.567082 | 7.4:-90766439 | None:intergenic |
| GGAATTTGGTCCGCATGTGG+AGG | 0.574852 | 7.4:-90765364 | MS.gene54530:CDS |
| AGACTATCACGAAATGAAGA+TGG | 0.578620 | 7.4:+90766354 | None:intergenic |
| ATTTGATTCAAGCACTTGAG+AGG | 0.587639 | 7.4:-90765072 | MS.gene54530:CDS |
| CTGCTCTTATTAGGACAGGA+AGG | 0.593973 | 7.4:-90765294 | MS.gene54530:CDS |
| TCTTATTAGGACAGGAAGGA+TGG | 0.597653 | 7.4:-90765290 | MS.gene54530:CDS |
| GAAGACAAGGAAGATAGTGT+AGG | 0.600054 | 7.4:-90765022 | MS.gene54530:CDS |
| TCAAGCACTTGAGAGGAAGA+AGG | 0.600486 | 7.4:-90765065 | MS.gene54530:CDS |
| AATTTGGTCCGCATGTGGAG+GGG | 0.605621 | 7.4:-90765362 | MS.gene54530:CDS |
| AGTGAACTATGGGCACAACT+AGG | 0.614578 | 7.4:-90766429 | MS.gene54530:CDS |
| AGGAACTGTGGAAGATGTGA+AGG | 0.615776 | 7.4:-90765002 | MS.gene54530:CDS |
| TTGCAATGAGAAATAGGCAA+AGG | 0.621519 | 7.4:-90765933 | MS.gene54530:CDS |
| AATCCTCTCCCCTCCACATG+CGG | 0.622031 | 7.4:+90765354 | None:intergenic |
| GGAAGATAGTGTAGGAACTG+TGG | 0.624422 | 7.4:-90765014 | MS.gene54530:CDS |
| AAAATTGAAACTTCATCACA+TGG | 0.627356 | 7.4:-90766069 | MS.gene54530:CDS |
| CTCAGTATTATCCTTAACCG+CGG | 0.645496 | 7.4:+90765615 | None:intergenic |
| AGCACTTGAGAGGAAGAAGG+TGG | 0.649097 | 7.4:-90765062 | MS.gene54530:CDS |
| GATGGAATTTGGTCCGCATG+TGG | 0.650290 | 7.4:-90765367 | MS.gene54530:CDS |
| GGCATACATGAACATTATGG+TGG | 0.652895 | 7.4:+90766395 | None:intergenic |
| GTTCATGAAATTCGCCATAG+CGG | 0.682175 | 7.4:+90765663 | None:intergenic |
| GTAGATTTACCTGTTCCTGG+AGG | 0.701492 | 7.4:+90765694 | None:intergenic |
| TTATGATCTTGAATTAACCG+CGG | 0.736452 | 7.4:-90765632 | MS.gene54530:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAATAGTAAAAATAGTA+AGG | - | chr7.4:90765971-90765990 | MS.gene54530:CDS | 10.0% |
| !! | AACAAAAAGATTATTATCAT+AGG | - | chr7.4:90765931-90765950 | MS.gene54530:CDS | 15.0% |
| !! | TTATTATCATAGGAAAAAAG+AGG | - | chr7.4:90765941-90765960 | MS.gene54530:CDS | 20.0% |
| !!! | CATTTTTATTAATCTTCCTT+TGG | + | chr7.4:90765881-90765900 | None:intergenic | 20.0% |
| !!! | GGTTTATTGAATTTTATTGA+TGG | - | chr7.4:90766005-90766024 | MS.gene54530:CDS | 20.0% |
| !!! | TGAATTTTATTGATGGAATT+TGG | - | chr7.4:90766012-90766031 | MS.gene54530:CDS | 20.0% |
| ! | AAAAGATAGTCAAAATCCAT+TGG | - | chr7.4:90765227-90765246 | MS.gene54530:CDS | 25.0% |
| ! | AAAATTGAAACTTCATCACA+TGG | - | chr7.4:90765321-90765340 | MS.gene54530:CDS | 25.0% |
| ! | AAGAAATTGCAATGAGAAAT+AGG | - | chr7.4:90765451-90765470 | MS.gene54530:CDS | 25.0% |
| ! | AAGGACTATTATGCAAAAAT+CGG | - | chr7.4:90765639-90765658 | MS.gene54530:CDS | 25.0% |
| ! | AATAGTAAGGTAACTTTATC+TGG | - | chr7.4:90765984-90766003 | MS.gene54530:CDS | 25.0% |
| ! | GGTTCTTGTTAAGAATTATT+TGG | - | chr7.4:90766166-90766185 | MS.gene54530:CDS | 25.0% |
| ! | TATACAAATAATCCAAGTAG+TGG | - | chr7.4:90765486-90765505 | MS.gene54530:CDS | 25.0% |
| ! | TGGTAAAAAACGTTCAATTA+TGG | + | chr7.4:90765019-90765038 | None:intergenic | 25.0% |
| !! | AATTTCAAGGTGTTAAACTT+TGG | - | chr7.4:90765286-90765305 | MS.gene54530:CDS | 25.0% |
| !! | AATTTTCACGACAAATTTTG+TGG | - | chr7.4:90766061-90766080 | MS.gene54530:CDS | 25.0% |
| !! | TTTGAAGTTTAGAAATGGTA+AGG | - | chr7.4:90765620-90765639 | MS.gene54530:CDS | 25.0% |
| !!! | CATGATTTGTTTGATGAAAT+TGG | - | chr7.4:90766200-90766219 | MS.gene54530:CDS | 25.0% |
| !!! | GATCTTTTGAAGTTTAGAAA+TGG | - | chr7.4:90765615-90765634 | MS.gene54530:CDS | 25.0% |
| !!! | TTGTTGTTTTGAAGCTTTTA+AGG | - | chr7.4:90766145-90766164 | MS.gene54530:CDS | 25.0% |
| AAACGCGGTTATTTACTTTA+TGG | - | chr7.4:90765672-90765691 | MS.gene54530:CDS | 30.0% | |
| AGAATTCATGGAAAGTGATT+TGG | + | chr7.4:90765107-90765126 | None:intergenic | 30.0% | |
| CTATTATGCAAAAATCGGTA+AGG | - | chr7.4:90765644-90765663 | MS.gene54530:CDS | 30.0% | |
| TATGGCATACATGAACATTA+TGG | + | chr7.4:90765001-90765020 | None:intergenic | 30.0% | |
| TTATGATCTTGAATTAACCG+CGG | - | chr7.4:90765758-90765777 | MS.gene54530:CDS | 30.0% | |
| TTTCATTGTGACAGTGAAAA+AGG | - | chr7.4:90766418-90766437 | MS.gene54530:CDS | 30.0% | |
| ! | TGATGAAATTGGTGATTTGT+TGG | - | chr7.4:90766211-90766230 | MS.gene54530:CDS | 30.0% |
| !!! | ATCAAGCATGTTTTGAAAGA+AGG | - | chr7.4:90765426-90765445 | MS.gene54530:CDS | 30.0% |
| !!! | TCAAGCATGTTTTGAAAGAA+GGG | - | chr7.4:90765427-90765446 | MS.gene54530:CDS | 30.0% |
| AACTTGATCCTGCTCTTATT+AGG | - | chr7.4:90766087-90766106 | MS.gene54530:CDS | 35.0% | |
| ACAAGAAGTAGAAGAAGACA+AGG | - | chr7.4:90766355-90766374 | MS.gene54530:CDS | 35.0% | |
| ACATCTTCATTCACAGACTT+AGG | + | chr7.4:90766281-90766300 | None:intergenic | 35.0% | |
| AGACTATCACGAAATGAAGA+TGG | + | chr7.4:90765039-90765058 | None:intergenic | 35.0% | |
| ATAGTAGATTTACCTGTTCC+TGG | + | chr7.4:90765702-90765721 | None:intergenic | 35.0% | |
| ATCACTTTCCATGAATTCTC+AGG | - | chr7.4:90765108-90765127 | MS.gene54530:CDS | 35.0% | |
| ATGGCTACAAACAATCCAAA+TGG | - | chr7.4:90765514-90765533 | MS.gene54530:CDS | 35.0% | |
| ATTTGATTCAAGCACTTGAG+AGG | - | chr7.4:90766318-90766337 | MS.gene54530:CDS | 35.0% | |
| CAAATAATCCAAGTAGTGGT+TGG | - | chr7.4:90765490-90765509 | MS.gene54530:CDS | 35.0% | |
| GAAAACAATGTGACTCCATT+TGG | + | chr7.4:90765532-90765551 | None:intergenic | 35.0% | |
| GAAGTCACTGATGAATTTCA+AGG | - | chr7.4:90765273-90765292 | MS.gene54530:CDS | 35.0% | |
| TATTTACTTTATGGTCCTCC+AGG | - | chr7.4:90765681-90765700 | MS.gene54530:CDS | 35.0% | |
| TCATCAATGCTAAGAACCAA+TGG | + | chr7.4:90765246-90765265 | None:intergenic | 35.0% | |
| TGATGAAGAAGACTCATTCA+TGG | - | chr7.4:90765908-90765927 | MS.gene54530:CDS | 35.0% | |
| TTGCAATGAGAAATAGGCAA+AGG | - | chr7.4:90765457-90765476 | MS.gene54530:CDS | 35.0% | |
| ! | CACTTGATTTTACTAGCCAA+AGG | - | chr7.4:90765862-90765881 | MS.gene54530:CDS | 35.0% |
| ! | GGAAAGTGATTTGGATGTAA+GGG | + | chr7.4:90765098-90765117 | None:intergenic | 35.0% |
| ! | TCAAGGTGTTAAACTTTGGT+GGG | - | chr7.4:90765290-90765309 | MS.gene54530:CDS | 35.0% |
| ! | TGGAAAGTGATTTGGATGTA+AGG | + | chr7.4:90765099-90765118 | None:intergenic | 35.0% |
| ! | TTCAAGGTGTTAAACTTTGG+TGG | - | chr7.4:90765289-90765308 | MS.gene54530:CDS | 35.0% |
| !! | TTTGAGTCTTTTGGCTAGTT+GGG | + | chr7.4:90765196-90765215 | None:intergenic | 35.0% |
| !!! | AACTTTTGAGACACTTGCAT+TGG | - | chr7.4:90765563-90765582 | MS.gene54530:CDS | 35.0% |
| AAATTCTCAGCAACATCAGC+AGG | + | chr7.4:90766254-90766273 | None:intergenic | 40.0% | |
| ATCTACTATGATAGCCGCTA+TGG | - | chr7.4:90765713-90765732 | MS.gene54530:CDS | 40.0% | |
| ATGCAAAAATCGGTAAGGCT+TGG | - | chr7.4:90765649-90765668 | MS.gene54530:CDS | 40.0% | |
| CTCAGTATTATCCTTAACCG+CGG | + | chr7.4:90765778-90765797 | None:intergenic | 40.0% | |
| GAACATTATGGTGGCTAGTA+TGG | + | chr7.4:90764989-90765008 | None:intergenic | 40.0% | |
| GAAGACAAGGAAGATAGTGT+AGG | - | chr7.4:90766368-90766387 | MS.gene54530:CDS | 40.0% | |
| GGCATACATGAACATTATGG+TGG | + | chr7.4:90764998-90765017 | None:intergenic | 40.0% | |
| GTCTTTCACCTGAGAATTCA+TGG | + | chr7.4:90765119-90765138 | None:intergenic | 40.0% | |
| GTGGAAGATGTGAAGGAAAA+TGG | - | chr7.4:90766395-90766414 | MS.gene54530:CDS | 40.0% | |
| GTTCATGAAATTCGCCATAG+CGG | + | chr7.4:90765730-90765749 | None:intergenic | 40.0% | |
| TCTTATTAGGACAGGAAGGA+TGG | - | chr7.4:90766100-90766119 | MS.gene54530:CDS | 40.0% | |
| TCTTGAATTAACCGCGGTTA+AGG | - | chr7.4:90765764-90765783 | MS.gene54530:CDS | 40.0% | |
| ! | AATCCAAGTAGTGGTTGGTA+TGG | - | chr7.4:90765495-90765514 | MS.gene54530:CDS | 40.0% |
| ! | GGATGAGTTTTCACTTAGGT+AGG | + | chr7.4:90765175-90765194 | None:intergenic | 40.0% |
| ! | GTTGGGATGAGTTTTCACTT+AGG | + | chr7.4:90765179-90765198 | None:intergenic | 40.0% |
| !! | CTTTGAGTCTTTTGGCTAGT+TGG | + | chr7.4:90765197-90765216 | None:intergenic | 40.0% |
| !!! | GACTTCAGCTTTGAGTCTTT+TGG | + | chr7.4:90765205-90765224 | None:intergenic | 40.0% |
| AGGAACTGTGGAAGATGTGA+AGG | - | chr7.4:90766388-90766407 | MS.gene54530:CDS | 45.0% | |
| AGTGAACTATGGGCACAACT+AGG | - | chr7.4:90764961-90764980 | MS.gene54530:CDS | 45.0% | |
| CTGCTCTTATTAGGACAGGA+AGG | - | chr7.4:90766096-90766115 | MS.gene54530:CDS | 45.0% | |
| CTTCCTGTCCTAATAAGAGC+AGG | + | chr7.4:90766098-90766117 | None:intergenic | 45.0% | |
| GATCCTGCTCTTATTAGGAC+AGG | - | chr7.4:90766092-90766111 | MS.gene54530:CDS | 45.0% | |
| GCAAGTGTCTCAAAAGTTGC+AGG | + | chr7.4:90765561-90765580 | None:intergenic | 45.0% | |
| GGAAGATAGTGTAGGAACTG+TGG | - | chr7.4:90766376-90766395 | MS.gene54530:CDS | 45.0% | |
| GTAGATTTACCTGTTCCTGG+AGG | + | chr7.4:90765699-90765718 | None:intergenic | 45.0% | |
| TAGCCATACCAACCACTACT+TGG | + | chr7.4:90765501-90765520 | None:intergenic | 45.0% | |
| TCAAGCACTTGAGAGGAAGA+AGG | - | chr7.4:90766325-90766344 | MS.gene54530:CDS | 45.0% | |
| AATTTGGTCCGCATGTGGAG+GGG | - | chr7.4:90766028-90766047 | MS.gene54530:CDS | 50.0% | |
| AGCACTTGAGAGGAAGAAGG+TGG | - | chr7.4:90766328-90766347 | MS.gene54530:CDS | 50.0% | |
| ATCGGTAAGGCTTGGAAACG+CGG | - | chr7.4:90765657-90765676 | MS.gene54530:CDS | 50.0% | |
| CTTTATGGTCCTCCAGGAAC+AGG | - | chr7.4:90765687-90765706 | MS.gene54530:CDS | 50.0% | |
| GAATTTGGTCCGCATGTGGA+GGG | - | chr7.4:90766027-90766046 | MS.gene54530:CDS | 50.0% | |
| GATGGAATTTGGTCCGCATG+TGG | - | chr7.4:90766023-90766042 | MS.gene54530:CDS | 50.0% | |
| AATCCTCTCCCCTCCACATG+CGG | + | chr7.4:90766039-90766058 | None:intergenic | 55.0% | |
| GGAATTTGGTCCGCATGTGG+AGG | - | chr7.4:90766026-90766045 | MS.gene54530:CDS | 55.0% | |
| GGTCCGCATGTGGAGGGGAG+AGG | - | chr7.4:90766033-90766052 | MS.gene54530:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 90764958 | 90766454 | 90764958 | ID=MS.gene54530 |
| chr7.4 | mRNA | 90764958 | 90766454 | 90764958 | ID=MS.gene54530.t1;Parent=MS.gene54530 |
| chr7.4 | exon | 90764958 | 90766454 | 90764958 | ID=MS.gene54530.t1.exon1;Parent=MS.gene54530.t1 |
| chr7.4 | CDS | 90764958 | 90766454 | 90764958 | ID=cds.MS.gene54530.t1;Parent=MS.gene54530.t1 |
| Gene Sequence |
| Protein sequence |
