Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57406.t1 | XP_003630571.1 | 97.1 | 139 | 4 | 0 | 1 | 139 | 1 | 139 | 2.40E-57 | 231.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57406.t1 | P52414 | 55.3 | 114 | 49 | 1 | 27 | 138 | 24 | 137 | 2.3e-24 | 113.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57406.t1 | G7LIR8 | 97.1 | 139 | 4 | 0 | 1 | 139 | 1 | 139 | 1.7e-57 | 231.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049491 | MS.gene57406 | 0.834384 | 3.09E-56 | -1.69E-46 |
| MS.gene049614 | MS.gene57406 | 0.814254 | 1.69E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene57406 | 0.801243 | 9.87E-49 | -1.69E-46 |
| MS.gene050076 | MS.gene57406 | 0.836727 | 7.90E-57 | -1.69E-46 |
| MS.gene050301 | MS.gene57406 | 0.831399 | 1.71E-55 | -1.69E-46 |
| MS.gene050767 | MS.gene57406 | 0.865708 | 4.85E-65 | -1.69E-46 |
| MS.gene050770 | MS.gene57406 | 0.865724 | 4.80E-65 | -1.69E-46 |
| MS.gene05101 | MS.gene57406 | 0.859185 | 4.93E-63 | -1.69E-46 |
| MS.gene051471 | MS.gene57406 | 0.83903 | 2.02E-57 | -1.69E-46 |
| MS.gene052078 | MS.gene57406 | 0.837376 | 5.39E-57 | -1.69E-46 |
| MS.gene052290 | MS.gene57406 | 0.826051 | 3.35E-54 | -1.69E-46 |
| MS.gene052293 | MS.gene57406 | 0.803384 | 3.58E-49 | -1.69E-46 |
| MS.gene052326 | MS.gene57406 | 0.879498 | 1.19E-69 | -1.69E-46 |
| MS.gene053322 | MS.gene57406 | 0.81859 | 1.81E-52 | -1.69E-46 |
| MS.gene054376 | MS.gene57406 | 0.869345 | 3.32E-66 | -1.69E-46 |
| MS.gene05455 | MS.gene57406 | 0.812526 | 4.06E-51 | -1.69E-46 |
| MS.gene054802 | MS.gene57406 | 0.842807 | 2.06E-58 | -1.69E-46 |
| MS.gene054803 | MS.gene57406 | 0.846357 | 2.28E-59 | -1.69E-46 |
| MS.gene054804 | MS.gene57406 | 0.84498 | 5.40E-59 | -1.69E-46 |
| MS.gene055254 | MS.gene57406 | 0.850193 | 1.99E-60 | -1.69E-46 |
| MS.gene055277 | MS.gene57406 | 0.832388 | 9.73E-56 | -1.69E-46 |
| MS.gene055390 | MS.gene57406 | 0.841181 | 5.55E-58 | -1.69E-46 |
| MS.gene055397 | MS.gene57406 | 0.834939 | 2.24E-56 | -1.69E-46 |
| MS.gene055422 | MS.gene57406 | 0.848445 | 6.10E-60 | -1.69E-46 |
| MS.gene055428 | MS.gene57406 | 0.803361 | 3.62E-49 | -1.69E-46 |
| MS.gene055499 | MS.gene57406 | 0.845772 | 3.30E-59 | -1.69E-46 |
| MS.gene055896 | MS.gene57406 | 0.830885 | 2.28E-55 | -1.69E-46 |
| MS.gene056931 | MS.gene57406 | 0.802393 | 5.73E-49 | -1.69E-46 |
| MS.gene057123 | MS.gene57406 | 0.819885 | 9.18E-53 | -1.69E-46 |
| MS.gene057129 | MS.gene57406 | 0.818684 | 1.72E-52 | -1.69E-46 |
| MS.gene057295 | MS.gene57406 | 0.807426 | 5.08E-50 | -1.69E-46 |
| MS.gene058293 | MS.gene57406 | 0.804392 | 2.21E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene57406 | 0.876266 | 1.60E-68 | -1.69E-46 |
| MS.gene059228 | MS.gene57406 | 0.81923 | 1.30E-52 | -1.69E-46 |
| MS.gene059326 | MS.gene57406 | 0.816287 | 5.98E-52 | -1.69E-46 |
| MS.gene059597 | MS.gene57406 | 0.829658 | 4.55E-55 | -1.69E-46 |
| MS.gene059960 | MS.gene57406 | 0.827373 | 1.62E-54 | -1.69E-46 |
| MS.gene060005 | MS.gene57406 | 0.811701 | 6.14E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene57406 | 0.809481 | 1.85E-50 | -1.69E-46 |
| MS.gene060559 | MS.gene57406 | 0.806297 | 8.81E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene57406 | 0.828945 | 6.78E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57406.t1 | MTR_8g099920 | 97.122 | 139 | 4 | 0 | 1 | 139 | 1 | 139 | 2.61e-95 | 271 |
| MS.gene57406.t1 | MTR_8g099920 | 95.683 | 139 | 4 | 1 | 1 | 139 | 1 | 137 | 6.45e-92 | 262 |
| MS.gene57406.t1 | MTR_8g099920 | 95.000 | 100 | 5 | 0 | 1 | 100 | 1 | 100 | 6.72e-64 | 191 |
| MS.gene57406.t1 | MTR_2g020360 | 47.552 | 143 | 65 | 4 | 1 | 138 | 1 | 138 | 4.10e-38 | 126 |
| MS.gene57406.t1 | MTR_2g020360 | 46.853 | 143 | 66 | 4 | 1 | 138 | 1 | 138 | 3.05e-36 | 121 |
| MS.gene57406.t1 | MTR_2g020360 | 51.220 | 123 | 52 | 4 | 22 | 138 | 18 | 138 | 5.29e-34 | 115 |
| MS.gene57406.t1 | MTR_4g118750 | 46.535 | 101 | 35 | 3 | 42 | 138 | 37 | 122 | 3.45e-21 | 83.6 |
| MS.gene57406.t1 | MTR_1g081920 | 50.000 | 80 | 35 | 2 | 56 | 133 | 10 | 86 | 7.93e-18 | 73.6 |
| MS.gene57406.t1 | MTR_7g080760 | 45.833 | 72 | 37 | 2 | 61 | 131 | 42 | 112 | 1.73e-11 | 57.8 |
| MS.gene57406.t1 | MTR_7g080390 | 41.772 | 79 | 44 | 2 | 61 | 138 | 53 | 130 | 4.83e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57406.t1 | AT4G25050 | 56.115 | 139 | 52 | 4 | 1 | 138 | 1 | 131 | 5.38e-40 | 131 |
| MS.gene57406.t1 | AT4G25050 | 51.656 | 151 | 52 | 5 | 1 | 138 | 1 | 143 | 1.49e-36 | 122 |
| MS.gene57406.t1 | AT3G05020 | 50.000 | 136 | 52 | 4 | 4 | 138 | 16 | 136 | 2.84e-34 | 116 |
| MS.gene57406.t1 | AT5G27200 | 58.163 | 98 | 38 | 2 | 42 | 138 | 42 | 137 | 1.20e-31 | 110 |
| MS.gene57406.t1 | AT1G54630 | 43.137 | 153 | 53 | 7 | 1 | 138 | 1 | 134 | 2.51e-28 | 101 |
| MS.gene57406.t1 | AT1G54580 | 57.647 | 85 | 34 | 1 | 54 | 138 | 52 | 134 | 1.13e-26 | 97.4 |
| MS.gene57406.t1 | AT1G65290 | 41.772 | 79 | 44 | 2 | 61 | 138 | 49 | 126 | 1.67e-11 | 58.2 |
| MS.gene57406.t1 | AT1G54630 | 53.448 | 58 | 25 | 1 | 54 | 111 | 52 | 107 | 3.12e-11 | 57.0 |
Find 24 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTCTGAAAATTTAGTTTC+TGG | 0.232292 | 4.1:-6759678 | None:intergenic |
| TCTGGGGTAAGCTTGGTTTC+AGG | 0.264840 | 4.1:-6759660 | None:intergenic |
| CATTATCATCCTCAATATTA+AGG | 0.281641 | 4.1:-6760013 | None:intergenic |
| CTCACTTTCTCCACTGTCTC+AGG | 0.295443 | 4.1:-6759606 | None:intergenic |
| TCTTTCAGAACTTCAAGTTT+TGG | 0.316075 | 4.1:+6759417 | MS.gene57406:CDS |
| GTTCTGAAAATTTAGTTTCT+GGG | 0.362213 | 4.1:-6759677 | None:intergenic |
| GGAAATAGTGATGGGTTTAG+AGG | 0.377032 | 4.1:+6759980 | MS.gene57406:CDS |
| TTTAGTTTCTGGGGTAAGCT+TGG | 0.464049 | 4.1:-6759667 | None:intergenic |
| CAAATCAGCTGCTTGTTGAA+TGG | 0.477053 | 4.1:-6760051 | None:intergenic |
| CCTCTTTCAATTTCTCTACA+AGG | 0.482096 | 4.1:+6758679 | MS.gene57406:CDS |
| AGAGTTTAACCTTAATATTG+AGG | 0.511328 | 4.1:+6760004 | MS.gene57406:CDS |
| GAAATTGAAAGAGGGTTGAA+AGG | 0.517599 | 4.1:-6758670 | None:intergenic |
| TTCTGAAAATTTAGTTTCTG+GGG | 0.526211 | 4.1:-6759676 | None:intergenic |
| CCTTGTAGAGAAATTGAAAG+AGG | 0.526756 | 4.1:-6758679 | None:intergenic |
| AGAGGGTTGAAAGGTAAGAG+AGG | 0.538850 | 4.1:-6758661 | None:intergenic |
| AAGAGAGGTGGTTGAAAGAG+AGG | 0.539433 | 4.1:-6758646 | None:intergenic |
| AAACGAGAAGATCTCAAAGA+AGG | 0.566334 | 4.1:-6759474 | None:intergenic |
| GGGTTGAAAGGTAAGAGAGG+TGG | 0.569945 | 4.1:-6758658 | None:intergenic |
| GTGGCAGGTGGAAATAGTGA+TGG | 0.572304 | 4.1:+6759971 | MS.gene57406:intron |
| CTTGTAGAGAAATTGAAAGA+GGG | 0.583035 | 4.1:-6758678 | None:intergenic |
| TCAGGCACAACCTGAGACAG+TGG | 0.583358 | 4.1:+6759596 | MS.gene57406:CDS |
| TTTCTCTACAAGGACTAACC+AGG | 0.601936 | 4.1:+6758689 | MS.gene57406:CDS |
| TGGCAGGTGGAAATAGTGAT+GGG | 0.642802 | 4.1:+6759972 | MS.gene57406:intron |
| TCAAACTTACTGCACAAATG+CGG | 0.672194 | 4.1:-6759497 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTTTTTTAAAAGATTC+AGG | + | chr4.1:6759578-6759597 | MS.gene57406:intron | 10.0% |
| !!! | GTTTTAATACTTTTTTTATG+AGG | + | chr4.1:6759342-6759361 | MS.gene57406:intron | 15.0% |
| !! | AAAGTCAAAATGAATTTCAA+AGG | - | chr4.1:6759526-6759545 | None:intergenic | 20.0% |
| !! | AAGTCAAAATGAATTTCAAA+GGG | - | chr4.1:6759525-6759544 | None:intergenic | 20.0% |
| !! | ACATACACATTAATATGTTA+AGG | + | chr4.1:6759124-6759143 | MS.gene57406:intron | 20.0% |
| !! | ATAAACAACAATTATCGTAT+AGG | + | chr4.1:6759205-6759224 | MS.gene57406:intron | 20.0% |
| !! | ATTTGTATTTACACTTCAAT+AGG | + | chr4.1:6758760-6758779 | MS.gene57406:intron | 20.0% |
| !! | TGAAAATAACTTATGAACAA+CGG | + | chr4.1:6759320-6759339 | MS.gene57406:intron | 20.0% |
| !!! | AAAAAATGTTACTATGCTTT+TGG | + | chr4.1:6758893-6758912 | MS.gene57406:intron | 20.0% |
| !!! | ACATTAATATGTTAAGGTTT+TGG | + | chr4.1:6759130-6759149 | MS.gene57406:intron | 20.0% |
| !!! | AGTGAAAATTTTAATTTGAG+TGG | + | chr4.1:6759374-6759393 | MS.gene57406:intron | 20.0% |
| !!! | ATTATTGGTTTTGATTTTGA+TGG | + | chr4.1:6759851-6759870 | MS.gene57406:intron | 20.0% |
| !!! | TGATTGATTCTTAATTAATG+TGG | + | chr4.1:6759952-6759971 | MS.gene57406:intron | 20.0% |
| !!! | TGTTTTGTTGTTTTTCTATT+TGG | + | chr4.1:6759182-6759201 | MS.gene57406:intron | 20.0% |
| ! | AAAAAAATATTGTACTCCGT+TGG | + | chr4.1:6759784-6759803 | MS.gene57406:intron | 25.0% |
| ! | AAAACCAATAATGTTCATTC+AGG | - | chr4.1:6759843-6759862 | None:intergenic | 25.0% |
| ! | AACATTGCATACAATAAATG+TGG | - | chr4.1:6758862-6758881 | None:intergenic | 25.0% |
| ! | AGAGTTTAACCTTAATATTG+AGG | + | chr4.1:6760004-6760023 | MS.gene57406:CDS | 25.0% |
| ! | AGTATATAGCATAGTTACAT+TGG | + | chr4.1:6759078-6759097 | MS.gene57406:intron | 25.0% |
| ! | ATTAAATGCATGAAATGACT+TGG | + | chr4.1:6758923-6758942 | MS.gene57406:intron | 25.0% |
| ! | CATTATCATCCTCAATATTA+AGG | - | chr4.1:6760016-6760035 | None:intergenic | 25.0% |
| ! | TATAGAGAAATTTCCAAAAG+TGG | - | chr4.1:6758973-6758992 | None:intergenic | 25.0% |
| !! | GAAACTAAATTTTCAGAACT+TGG | + | chr4.1:6759681-6759700 | MS.gene57406:CDS | 25.0% |
| !! | TTCTGAAAATTTAGTTTCTG+GGG | - | chr4.1:6759679-6759698 | None:intergenic | 25.0% |
| !!! | AATACTTTTTTTATGAGGTG+AGG | + | chr4.1:6759347-6759366 | MS.gene57406:intron | 25.0% |
| !!! | AGTTCTGAAAATTTAGTTTC+TGG | - | chr4.1:6759681-6759700 | None:intergenic | 25.0% |
| !!! | GTTCTGAAAATTTAGTTTCT+GGG | - | chr4.1:6759680-6759699 | None:intergenic | 25.0% |
| !!! | TCCAAAAGTGGTTTATTTTT+CGG | - | chr4.1:6758961-6758980 | None:intergenic | 25.0% |
| !!! | TGACTTTTAAAAAACTTGCA+AGG | + | chr4.1:6759539-6759558 | MS.gene57406:intron | 25.0% |
| CCAACACCTAAATTATCAAT+TGG | - | chr4.1:6759051-6759070 | None:intergenic | 30.0% | |
| CTTGTAGAGAAATTGAAAGA+GGG | - | chr4.1:6758681-6758700 | None:intergenic | 30.0% | |
| GTAAGTATATGCATTAAGAG+TGG | - | chr4.1:6758731-6758750 | None:intergenic | 30.0% | |
| GTTTGACATTTGATATGTTG+TGG | + | chr4.1:6758805-6758824 | MS.gene57406:intron | 30.0% | |
| TATAGCATAGTTACATTGGT+AGG | + | chr4.1:6759082-6759101 | MS.gene57406:intron | 30.0% | |
| TGATTCTTAATTAATGTGGC+AGG | + | chr4.1:6759956-6759975 | MS.gene57406:intron | 30.0% | |
| ! | CCAATTGATAATTTAGGTGT+TGG | + | chr4.1:6759048-6759067 | MS.gene57406:intron | 30.0% |
| ! | GAGTGTCCAATTGATAATTT+AGG | + | chr4.1:6759042-6759061 | MS.gene57406:intron | 30.0% |
| ! | TCCGAAAAATAAACCACTTT+TGG | + | chr4.1:6758957-6758976 | MS.gene57406:intron | 30.0% |
| !! | TCTTTCAGAACTTCAAGTTT+TGG | + | chr4.1:6759417-6759436 | MS.gene57406:CDS | 30.0% |
| !!! | TTTGATTTTGATGGTGCATT+TGG | + | chr4.1:6759860-6759879 | MS.gene57406:intron | 30.0% |
| AAACGAGAAGATCTCAAAGA+AGG | - | chr4.1:6759477-6759496 | None:intergenic | 35.0% | |
| AGTGGTTAAAACATACATGC+AGG | + | chr4.1:6759392-6759411 | MS.gene57406:intron | 35.0% | |
| CCTCTTTCAATTTCTCTACA+AGG | + | chr4.1:6758679-6758698 | MS.gene57406:CDS | 35.0% | |
| CCTTGTAGAGAAATTGAAAG+AGG | - | chr4.1:6758682-6758701 | None:intergenic | 35.0% | |
| GAAATTGAAAGAGGGTTGAA+AGG | - | chr4.1:6758673-6758692 | None:intergenic | 35.0% | |
| GAACTTGACCTTATAACCAA+CGG | - | chr4.1:6759803-6759822 | None:intergenic | 35.0% | |
| GACACCTGAATGAACATTAT+TGG | + | chr4.1:6759836-6759855 | MS.gene57406:intron | 35.0% | |
| GTTGTTTATGCTAGAGCTAT+GGG | + | chr4.1:6759918-6759937 | MS.gene57406:intron | 35.0% | |
| TCAAACTTACTGCACAAATG+CGG | - | chr4.1:6759500-6759519 | None:intergenic | 35.0% | |
| TTCTTAATTAATGTGGCAGG+TGG | + | chr4.1:6759959-6759978 | MS.gene57406:intron | 35.0% | |
| ! | ATTGTACTCCGTTGGTTATA+AGG | + | chr4.1:6759792-6759811 | MS.gene57406:intron | 35.0% |
| ! | TGTTGTTTATGCTAGAGCTA+TGG | + | chr4.1:6759917-6759936 | MS.gene57406:intron | 35.0% |
| !! | AAGTTTTGGTGCAATTTCTG+TGG | + | chr4.1:6759431-6759450 | MS.gene57406:CDS | 35.0% |
| !! | AGTTTTGGTGCAATTTCTGT+GGG | + | chr4.1:6759432-6759451 | MS.gene57406:CDS | 35.0% |
| ACACGTGCAAATTAAACGCA+CGG | + | chr4.1:6759011-6759030 | MS.gene57406:intron | 40.0% | |
| GGAAGATGAAAATCTGAACC+TGG | - | chr4.1:6758710-6758729 | None:intergenic | 40.0% | |
| TTTCTCTACAAGGACTAACC+AGG | + | chr4.1:6758689-6758708 | MS.gene57406:CDS | 40.0% | |
| ! | CAAATCAGCTGCTTGTTGAA+TGG | - | chr4.1:6760054-6760073 | None:intergenic | 40.0% |
| !! | GGAAATAGTGATGGGTTTAG+AGG | + | chr4.1:6759980-6759999 | MS.gene57406:CDS | 40.0% |
| !! | TTTAGTTTCTGGGGTAAGCT+TGG | - | chr4.1:6759670-6759689 | None:intergenic | 40.0% |
| AAGAGAGGTGGTTGAAAGAG+AGG | - | chr4.1:6758649-6758668 | None:intergenic | 45.0% | |
| AATTAAACGCACGGACGTGA+AGG | + | chr4.1:6759020-6759039 | MS.gene57406:intron | 45.0% | |
| AGAGGGTTGAAAGGTAAGAG+AGG | - | chr4.1:6758664-6758683 | None:intergenic | 45.0% | |
| CATTTGATATGTTGTGGCGC+AGG | + | chr4.1:6758811-6758830 | MS.gene57406:intron | 45.0% | |
| TGGCAGGTGGAAATAGTGAT+GGG | + | chr4.1:6759972-6759991 | MS.gene57406:intron | 45.0% | |
| CTCACTTTCTCCACTGTCTC+AGG | - | chr4.1:6759609-6759628 | None:intergenic | 50.0% | |
| GGGTTGAAAGGTAAGAGAGG+TGG | - | chr4.1:6758661-6758680 | None:intergenic | 50.0% | |
| GTGGCAGGTGGAAATAGTGA+TGG | + | chr4.1:6759971-6759990 | MS.gene57406:intron | 50.0% | |
| TCAGGTGTCAGCTCAGTGAT+AGG | - | chr4.1:6759825-6759844 | None:intergenic | 50.0% | |
| ! | TCTGGGGTAAGCTTGGTTTC+AGG | - | chr4.1:6759663-6759682 | None:intergenic | 50.0% |
| TCAGGCACAACCTGAGACAG+TGG | + | chr4.1:6759596-6759615 | MS.gene57406:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 6758642 | 6760103 | 6758642 | ID=MS.gene57406 |
| chr4.1 | mRNA | 6758642 | 6760103 | 6758642 | ID=MS.gene57406.t1;Parent=MS.gene57406 |
| chr4.1 | exon | 6758642 | 6758710 | 6758642 | ID=MS.gene57406.t1.exon1;Parent=MS.gene57406.t1 |
| chr4.1 | CDS | 6758642 | 6758710 | 6758642 | ID=cds.MS.gene57406.t1;Parent=MS.gene57406.t1 |
| chr4.1 | exon | 6759414 | 6759509 | 6759414 | ID=MS.gene57406.t1.exon2;Parent=MS.gene57406.t1 |
| chr4.1 | CDS | 6759414 | 6759509 | 6759414 | ID=cds.MS.gene57406.t1;Parent=MS.gene57406.t1 |
| chr4.1 | exon | 6759594 | 6759722 | 6759594 | ID=MS.gene57406.t1.exon3;Parent=MS.gene57406.t1 |
| chr4.1 | CDS | 6759594 | 6759722 | 6759594 | ID=cds.MS.gene57406.t1;Parent=MS.gene57406.t1 |
| chr4.1 | exon | 6759978 | 6760103 | 6759978 | ID=MS.gene57406.t1.exon4;Parent=MS.gene57406.t1 |
| chr4.1 | CDS | 6759978 | 6760103 | 6759978 | ID=cds.MS.gene57406.t1;Parent=MS.gene57406.t1 |
| Gene Sequence |
| Protein sequence |
