AlfalfaGEDB | MS.gene57813

M. sativa cultivar XinJiangDaYe / MS.gene57813

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00197.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57813.t1	XP_003620187.1	96.2	211	8	0	1	211	1	211	2.00E-116	428.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57813.t1	P13087	40.8	211	104	6	11	211	17	216	4.4e-35	149.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57813.t1	G7KKQ9	96.2	211	8	0	1	211	1	211	1.5e-116	428.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049758	MS.gene57813	-0.809253	2.07E-50	-1.69E-46
MS.gene050397	MS.gene57813	-0.815955	7.09E-52	-1.69E-46
MS.gene050464	MS.gene57813	-0.818085	2.36E-52	-1.69E-46
MS.gene050529	MS.gene57813	-0.814563	1.45E-51	-1.69E-46
MS.gene050670	MS.gene57813	-0.800879	1.17E-48	-1.69E-46
MS.gene050971	MS.gene57813	-0.816771	4.66E-52	-1.69E-46
MS.gene051860	MS.gene57813	-0.82836	9.39E-55	-1.69E-46
MS.gene051983	MS.gene57813	-0.801606	8.32E-49	-1.69E-46
MS.gene052011	MS.gene57813	0.829922	3.93E-55	-1.69E-46
MS.gene052188	MS.gene57813	-0.820837	5.55E-53	-1.69E-46
MS.gene052253	MS.gene57813	-0.827733	1.33E-54	-1.69E-46
MS.gene05233	MS.gene57813	0.92821	3.98E-92	-1.69E-46
MS.gene052340	MS.gene57813	-0.805008	1.64E-49	-1.69E-46
MS.gene053836	MS.gene57813	-0.818668	1.74E-52	-1.69E-46
MS.gene054183	MS.gene57813	-0.835968	1.23E-56	-1.69E-46
MS.gene054349	MS.gene57813	-0.805163	1.52E-49	-1.69E-46
MS.gene055422	MS.gene57813	-0.802845	4.62E-49	-1.69E-46
MS.gene055601	MS.gene57813	0.867993	9.09E-66	-1.69E-46
MS.gene056330	MS.gene57813	0.815199	1.04E-51	-1.69E-46
MS.gene056690	MS.gene57813	0.802021	6.84E-49	-1.69E-46
MS.gene056899	MS.gene57813	0.880186	6.75E-70	-1.69E-46
MS.gene057779	MS.gene57813	-0.80612	9.60E-50	-1.69E-46
MS.gene058002	MS.gene57813	-0.805963	1.04E-49	-1.69E-46
MS.gene05811	MS.gene57813	-0.803926	2.76E-49	-1.69E-46
MS.gene058218	MS.gene57813	0.846334	2.32E-59	-1.69E-46
MS.gene058660	MS.gene57813	-0.804105	2.53E-49	-1.69E-46
MS.gene058710	MS.gene57813	-0.803347	3.64E-49	-1.69E-46
MS.gene058928	MS.gene57813	-0.814911	1.21E-51	-1.69E-46
MS.gene059043	MS.gene57813	0.809287	2.04E-50	-1.69E-46
MS.gene05907	MS.gene57813	-0.816001	6.93E-52	-1.69E-46
MS.gene059285	MS.gene57813	-0.807768	4.30E-50	-1.69E-46
MS.gene059294	MS.gene57813	-0.826637	2.43E-54	-1.69E-46
MS.gene059420	MS.gene57813	-0.82943	5.17E-55	-1.69E-46
MS.gene05984	MS.gene57813	-0.833892	4.11E-56	-1.69E-46
MS.gene059894	MS.gene57813	-0.800447	1.43E-48	-1.69E-46
MS.gene061049	MS.gene57813	-0.84225	2.90E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57813.t1	MTR_6g078280	95.755	212	9	0	1	212	1	212	1.37e-144	401
MS.gene57813.t1	MTR_6g078260	69.500	200	41	5	15	211	16	198	5.75e-95	275
MS.gene57813.t1	MTR_6g078140	59.701	201	73	5	15	211	16	212	2.42e-74	224
MS.gene57813.t1	MTR_6g078120	60.199	201	72	5	15	211	16	212	1.66e-73	221
MS.gene57813.t1	MTR_6g078290	53.023	215	91	6	1	210	1	210	3.97e-68	208
MS.gene57813.t1	MTR_6g078250	53.023	215	87	5	1	212	1	204	2.11e-65	201
MS.gene57813.t1	MTR_7g037410	44.444	189	90	5	26	211	25	201	6.11e-50	161
MS.gene57813.t1	MTR_6g078100	38.462	221	109	10	1	211	1	204	1.16e-35	125
MS.gene57813.t1	MTR_6g078070	37.156	218	114	9	1	211	1	202	3.96e-32	115
MS.gene57813.t1	MTR_6g009563	30.097	206	121	7	3	203	6	193	5.10e-13	65.9
MS.gene57813.t1	MTR_8g461050	30.189	212	123	10	9	211	6	201	5.12e-13	65.5
MS.gene57813.t1	MTR_0211s0080	29.108	213	120	10	9	211	6	197	5.90e-13	65.5
MS.gene57813.t1	MTR_3g106620	30.667	225	112	13	6	212	1	199	2.27e-12	63.9
MS.gene57813.t1	MTR_0084s0030	29.907	214	121	12	9	211	6	201	3.41e-12	63.2
MS.gene57813.t1	MTR_6g009650	31.053	190	107	7	23	203	18	192	6.53e-12	62.8
MS.gene57813.t1	MTR_6g066190	30.000	190	109	7	23	203	18	192	1.68e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57813.t1	AT1G17860	37.963	216	107	9	1	211	1	194	3.91e-39	133
MS.gene57813.t1	AT1G73325	32.889	225	114	11	1	211	1	202	5.51e-22	90.1
MS.gene57813.t1	AT1G73260	31.280	211	122	11	8	212	12	205	4.20e-20	84.7
MS.gene57813.t1	AT1G73330	30.317	221	116	13	1	212	1	192	9.91e-15	70.5

Find 52 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCTGAAACTTAACTTTAA+AGG	0.154767	6.1:-69323939	None:intergenic
CATCTTCATACTTCTCAATT+TGG	0.163418	6.1:-69323802	None:intergenic
ACAAAGCCACATATTGTAAA+TGG	0.283069	6.1:-69323479	None:intergenic
AATGTTTCTTCTTTAGAAAA+AGG	0.285974	6.1:-69323722	None:intergenic
TTCTGAAACTTAACTTTAAA+GGG	0.298093	6.1:-69323938	None:intergenic
AGCCGGAATGATGTAGTAAT+TGG	0.358723	6.1:-69323453	None:intergenic
AACCATGTATGCAAAGATAT+TGG	0.371437	6.1:+69323866	MS.gene57813:CDS
AACGCCTTTCTTGTTGTTAA+CGG	0.386453	6.1:-69323609	None:intergenic
GTGACTACTAATGGTGTATT+GGG	0.404413	6.1:+69323746	MS.gene57813:CDS
ACGCCTTTCTTGTTGTTAAC+GGG	0.404872	6.1:-69323608	None:intergenic
TTCCAATATCTTTGCATACA+TGG	0.418385	6.1:-69323868	None:intergenic
GAAACATTTGTGACTACTAA+TGG	0.419288	6.1:+69323737	MS.gene57813:CDS
TGCATACATGGTTGCAAGTA+GGG	0.429484	6.1:-69323856	None:intergenic
GCTAAGCCATTTACAATATG+TGG	0.430563	6.1:+69323473	MS.gene57813:CDS
TGTGACTACTAATGGTGTAT+TGG	0.442930	6.1:+69323745	MS.gene57813:CDS
TACCAATTACTACATCATTC+CGG	0.457107	6.1:+69323451	MS.gene57813:CDS
TAAGACTAGGGAAATGCGTT+TGG	0.471251	6.1:+69323901	MS.gene57813:CDS
TTTGTTAGTTGTTTCAATAG+TGG	0.473075	6.1:+69323497	MS.gene57813:CDS
GTTCAAATACAATCCACAAT+TGG	0.476445	6.1:+69323777	MS.gene57813:CDS
CATATTGTAAATGGCTTAGC+CGG	0.479615	6.1:-69323470	None:intergenic
TCTCAATTTGGAACCAATTG+TGG	0.486271	6.1:-69323790	None:intergenic
TTGTCCTCGCAACCACTACT+TGG	0.487006	6.1:+69323368	MS.gene57813:CDS
CGATGTGTCAATGACTTGAT+CGG	0.502159	6.1:-69323408	None:intergenic
GAACTTTATGTTGAGATCAG+TGG	0.505826	6.1:-69323642	None:intergenic
TTGCAAGTAGGGCACACATT+AGG	0.510934	6.1:-69323845	None:intergenic
GAATTTATGTGTATAAGACT+AGG	0.515322	6.1:+69323888	MS.gene57813:CDS
TTGCATACATGGTTGCAAGT+AGG	0.520717	6.1:-69323857	None:intergenic
GTAGTTGTGAAACATAATCA+AGG	0.526996	6.1:+69323566	MS.gene57813:CDS
AATTTATGTGTATAAGACTA+GGG	0.531831	6.1:+69323889	MS.gene57813:CDS
AGGATCAATCTTCCACACTA+AGG	0.534325	6.1:-69323702	None:intergenic
TTGTGGATTGTATTTGAACC+CGG	0.536118	6.1:-69323773	None:intergenic
GTATGAAGATGCTTATAAGT+TGG	0.537055	6.1:+69323814	MS.gene57813:CDS
ATAATCAAGGTTTACCGTTG+AGG	0.542322	6.1:+69323579	MS.gene57813:CDS
TTCCACACTAAGGAATGGTT+AGG	0.542536	6.1:-69323692	None:intergenic
GTTAGTTGTTTCAATAGTGG+AGG	0.542635	6.1:+69323500	MS.gene57813:CDS
GATGTGTCAATGACTTGATC+GGG	0.551157	6.1:-69323407	None:intergenic
TAATGGTGTATTGGGAAACC+CGG	0.551726	6.1:+69323754	MS.gene57813:CDS
CTTTAAAGTTAAGTTTCAGA+AGG	0.553919	6.1:+69323940	MS.gene57813:CDS
GTCCTAACCATTCCTTAGTG+TGG	0.567409	6.1:+69323690	MS.gene57813:CDS
ACACCCGTTAACAACAAGAA+AGG	0.576147	6.1:+69323605	MS.gene57813:CDS
GATCAAGTCATTGACACATC+GGG	0.588958	6.1:+69323410	MS.gene57813:CDS
TTCACAACTACTACATCAAG+AGG	0.592646	6.1:-69323554	None:intergenic
GGCATTGCTCTTGAAGCCGT+CGG	0.593880	6.1:+69323521	MS.gene57813:CDS
CAATCTTCCACACTAAGGAA+TGG	0.596624	6.1:-69323697	None:intergenic
TGTGGATTGTATTTGAACCC+GGG	0.605984	6.1:-69323772	None:intergenic
GCTTCAGTTGATCCAAGTAG+TGG	0.616203	6.1:-69323380	None:intergenic
CGATCAAGTCATTGACACAT+CGG	0.616565	6.1:+69323409	MS.gene57813:CDS
TTAACGGGTGTGAACCTCAA+CGG	0.621698	6.1:-69323593	None:intergenic
TGATCCAAGTAGTGGTTGCG+AGG	0.644309	6.1:-69323372	None:intergenic
AAGAGGGCAAGATTCTCCGA+CGG	0.665716	6.1:-69323537	None:intergenic
AATGGTGTATTGGGAAACCC+GGG	0.666072	6.1:+69323755	MS.gene57813:CDS
TCACAACTACTACATCAAGA+GGG	0.703659	6.1:-69323553	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTTATGTGTATAAGACTA+GGG	+	chr6.1:69323889-69323908	MS.gene57813:CDS	20.0%
!!!	AATGTTTCTTCTTTAGAAAA+AGG	-	chr6.1:69323725-69323744	None:intergenic	20.0%
!!!	TTCTGAAACTTAACTTTAAA+GGG	-	chr6.1:69323941-69323960	None:intergenic	20.0%
!	GAATTTATGTGTATAAGACT+AGG	+	chr6.1:69323888-69323907	MS.gene57813:CDS	25.0%
!!	TTTGTTAGTTGTTTCAATAG+TGG	+	chr6.1:69323497-69323516	MS.gene57813:CDS	25.0%
!!!	CTTCTGAAACTTAACTTTAA+AGG	-	chr6.1:69323942-69323961	None:intergenic	25.0%
!!!	CTTTAAAGTTAAGTTTCAGA+AGG	+	chr6.1:69323940-69323959	MS.gene57813:CDS	25.0%
	AACCATGTATGCAAAGATAT+TGG	+	chr6.1:69323866-69323885	MS.gene57813:CDS	30.0%
	ACAAAGCCACATATTGTAAA+TGG	-	chr6.1:69323482-69323501	None:intergenic	30.0%
	CATCTTCATACTTCTCAATT+TGG	-	chr6.1:69323805-69323824	None:intergenic	30.0%
	GAAACATTTGTGACTACTAA+TGG	+	chr6.1:69323737-69323756	MS.gene57813:CDS	30.0%
	GTAGTTGTGAAACATAATCA+AGG	+	chr6.1:69323566-69323585	MS.gene57813:CDS	30.0%
	GTATGAAGATGCTTATAAGT+TGG	+	chr6.1:69323814-69323833	MS.gene57813:CDS	30.0%
	GTTCAAATACAATCCACAAT+TGG	+	chr6.1:69323777-69323796	MS.gene57813:CDS	30.0%
	TACCAATTACTACATCATTC+CGG	+	chr6.1:69323451-69323470	MS.gene57813:CDS	30.0%
	TTCCAATATCTTTGCATACA+TGG	-	chr6.1:69323871-69323890	None:intergenic	30.0%
	ATAATCAAGGTTTACCGTTG+AGG	+	chr6.1:69323579-69323598	MS.gene57813:CDS	35.0%
	CATATTGTAAATGGCTTAGC+CGG	-	chr6.1:69323473-69323492	None:intergenic	35.0%
	GAACTTTATGTTGAGATCAG+TGG	-	chr6.1:69323645-69323664	None:intergenic	35.0%
	GCTAAGCCATTTACAATATG+TGG	+	chr6.1:69323473-69323492	MS.gene57813:CDS	35.0%
	GTGACTACTAATGGTGTATT+GGG	+	chr6.1:69323746-69323765	MS.gene57813:CDS	35.0%
	TCACAACTACTACATCAAGA+GGG	-	chr6.1:69323556-69323575	None:intergenic	35.0%
	TGTGACTACTAATGGTGTAT+TGG	+	chr6.1:69323745-69323764	MS.gene57813:CDS	35.0%
	TTCACAACTACTACATCAAG+AGG	-	chr6.1:69323557-69323576	None:intergenic	35.0%
!	GTTAGTTGTTTCAATAGTGG+AGG	+	chr6.1:69323500-69323519	MS.gene57813:CDS	35.0%
!	TCTCAATTTGGAACCAATTG+TGG	-	chr6.1:69323793-69323812	None:intergenic	35.0%
!	TTGTGGATTGTATTTGAACC+CGG	-	chr6.1:69323776-69323795	None:intergenic	35.0%
!!	AACGCCTTTCTTGTTGTTAA+CGG	-	chr6.1:69323612-69323631	None:intergenic	35.0%
	ACACCCGTTAACAACAAGAA+AGG	+	chr6.1:69323605-69323624	MS.gene57813:CDS	40.0%
	AGCCGGAATGATGTAGTAAT+TGG	-	chr6.1:69323456-69323475	None:intergenic	40.0%
	AGGATCAATCTTCCACACTA+AGG	-	chr6.1:69323705-69323724	None:intergenic	40.0%
	CAATCTTCCACACTAAGGAA+TGG	-	chr6.1:69323700-69323719	None:intergenic	40.0%
	CGATCAAGTCATTGACACAT+CGG	+	chr6.1:69323409-69323428	MS.gene57813:CDS	40.0%
	CGATGTGTCAATGACTTGAT+CGG	-	chr6.1:69323411-69323430	None:intergenic	40.0%
	GATCAAGTCATTGACACATC+GGG	+	chr6.1:69323410-69323429	MS.gene57813:CDS	40.0%
	GATGTGTCAATGACTTGATC+GGG	-	chr6.1:69323410-69323429	None:intergenic	40.0%
	TAAGACTAGGGAAATGCGTT+TGG	+	chr6.1:69323901-69323920	MS.gene57813:CDS	40.0%
	TAATGGTGTATTGGGAAACC+CGG	+	chr6.1:69323754-69323773	MS.gene57813:CDS	40.0%
	TGCATACATGGTTGCAAGTA+GGG	-	chr6.1:69323859-69323878	None:intergenic	40.0%
	TTCCACACTAAGGAATGGTT+AGG	-	chr6.1:69323695-69323714	None:intergenic	40.0%
	TTGCATACATGGTTGCAAGT+AGG	-	chr6.1:69323860-69323879	None:intergenic	40.0%
!	TGTGGATTGTATTTGAACCC+GGG	-	chr6.1:69323775-69323794	None:intergenic	40.0%
!!	ACGCCTTTCTTGTTGTTAAC+GGG	-	chr6.1:69323611-69323630	None:intergenic	40.0%
	AATGGTGTATTGGGAAACCC+GGG	+	chr6.1:69323755-69323774	MS.gene57813:CDS	45.0%
	GCTTCAGTTGATCCAAGTAG+TGG	-	chr6.1:69323383-69323402	None:intergenic	45.0%
	GTCCTAACCATTCCTTAGTG+TGG	+	chr6.1:69323690-69323709	MS.gene57813:CDS	45.0%
	TTGCAAGTAGGGCACACATT+AGG	-	chr6.1:69323848-69323867	None:intergenic	45.0%
!	TTAACGGGTGTGAACCTCAA+CGG	-	chr6.1:69323596-69323615	None:intergenic	45.0%
	AAGAGGGCAAGATTCTCCGA+CGG	-	chr6.1:69323540-69323559	None:intergenic	50.0%
	TGATCCAAGTAGTGGTTGCG+AGG	-	chr6.1:69323375-69323394	None:intergenic	50.0%
	TTGTCCTCGCAACCACTACT+TGG	+	chr6.1:69323368-69323387	MS.gene57813:CDS	50.0%
	GGCATTGCTCTTGAAGCCGT+CGG	+	chr6.1:69323521-69323540	MS.gene57813:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	69323329	69323967	69323329	ID=MS.gene57813
chr6.1	mRNA	69323329	69323967	69323329	ID=MS.gene57813.t1;Parent=MS.gene57813
chr6.1	exon	69323329	69323967	69323329	ID=MS.gene57813.t1.exon1;Parent=MS.gene57813.t1
chr6.1	CDS	69323329	69323967	69323329	ID=cds.MS.gene57813.t1;Parent=MS.gene57813.t1

Gene Sequence

>MS.gene57813

ATGAAGAGTACAATGCTAGCATTTCTTCTTCTCTTTGCATTGTCCTCGCAACCACTACTTGGATCAACTGAAGCTTCACCCGATCAAGTCATTGACACATCGGGCAAGAAGCTTCGAGCTGATACCAATTACTACATCATTCCGGCTAAGCCATTTACAATATGTGGCTTTGTTAGTTGTTTCAATAGTGGAGGCATTGCTCTTGAAGCCGTCGGAGAATCTTGCCCTCTTGATGTAGTAGTTGTGAAACATAATCAAGGTTTACCGTTGAGGTTCACACCCGTTAACAACAAGAAAGGCGTTATTCGTGTCTCCACTGATCTCAACATAAAGTTCTCTAATGATGCTTATGATTCTAGATGTCCTAACCATTCCTTAGTGTGGAAGATTGATCCTTTTTCTAAAGAAGAAACATTTGTGACTACTAATGGTGTATTGGGAAACCCGGGTTCAAATACAATCCACAATTGGTTCCAAATTGAGAAGTATGAAGATGCTTATAAGTTGGTCTATTGTCCTAATGTGTGCCCTACTTGCAACCATGTATGCAAAGATATTGGAATTTATGTGTATAAGACTAGGGAAATGCGTTTGGCTCTCACTAATGTTCCCTTTAAAGTTAAGTTTCAGAAGGTTTGA

Protein sequence

>MS.gene57813.t1

MKSTMLAFLLLFALSSQPLLGSTEASPDQVIDTSGKKLRADTNYYIIPAKPFTICGFVSCFNSGGIALEAVGESCPLDVVVVKHNQGLPLRFTPVNNKKGVIRVSTDLNIKFSNDAYDSRCPNHSLVWKIDPFSKEETFVTTNGVLGNPGSNTIHNWFQIEKYEDAYKLVYCPNVCPTCNHVCKDIGIYVYKTREMRLALTNVPFKVKFQKV