AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57991


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57991.t1 MTR_4g116130 94.527 201 11 0 1 201 1 201 4.95e-137 381
MS.gene57991.t1 MTR_4g116140 85.149 202 29 1 1 201 1 202 6.38e-123 346
MS.gene57991.t1 MTR_0182s0050 84.239 184 29 0 18 201 5 188 1.91e-110 313
MS.gene57991.t1 MTR_1g115240 71.144 201 30 1 1 201 1 173 3.71e-96 277
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57991.t1 AT2G11890 55.714 210 84 1 1 201 1 210 2.07e-66 203
MS.gene57991.t1 AT2G11890 47.143 210 65 2 1 201 1 173 2.37e-47 153

Find 56 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGGGTGGTGGGTGAAAA+TGG 0.155699 8.3:-10306493 MS.gene57991:CDS
TGAAAATGGGTTTGTGGGTT+TGG 0.196016 8.3:-10306479 MS.gene57991:CDS
GGGAGGGTGAAGGATGAATT+TGG 0.200304 8.3:-10306511 MS.gene57991:CDS
GTTGGTCGAGTCAAGGATTA+TGG 0.236549 8.3:-10306533 MS.gene57991:CDS
GAAAATGGGTTTGTGGGTTT+GGG 0.245959 8.3:-10306478 MS.gene57991:CDS
TGAAGAAGATGAGGAGGATT+TGG 0.275997 8.3:-10306602 MS.gene57991:CDS
TCGGGATTGCGTTGCTGAAC+CGG 0.298448 8.3:-10306566 MS.gene57991:CDS
GTTGAAAGCAAAGGCGGTTT+TGG 0.306375 8.3:-10306644 MS.gene57991:CDS
TGATTGGAAAGGTTTGAAAT+TGG 0.318001 8.3:-10306428 MS.gene57991:CDS
GTTGCTGAACCGGGGAAGTT+GGG 0.338043 8.3:-10306556 MS.gene57991:CDS
GAAGGATGAATTTGGGGTGG+TGG 0.339048 8.3:-10306503 MS.gene57991:CDS
GGTGGGTGAAAATGGGTTTG+TGG 0.340645 8.3:-10306485 MS.gene57991:CDS
GGAGGGTGAAGGATGAATTT+GGG 0.368591 8.3:-10306510 MS.gene57991:CDS
TTGGGGTGGTGGGTGAAAAT+GGG 0.381102 8.3:-10306492 MS.gene57991:CDS
GTGGGTGAAAATGGGTTTGT+GGG 0.382924 8.3:-10306484 MS.gene57991:CDS
AATGGGTTTGTGGGTTTGGG+AGG 0.407882 8.3:-10306475 MS.gene57991:CDS
CGTTGCTGAACCGGGGAAGT+TGG 0.412112 8.3:-10306557 MS.gene57991:CDS
GAGGATTTGGATCCGAAGAT+TGG 0.424344 8.3:-10306589 MS.gene57991:CDS
AATCTCTTCTTCGACGGCGC+TGG 0.426538 8.3:-10306739 MS.gene57991:CDS
CGGGATTGCGTTGCTGAACC+GGG 0.433058 8.3:-10306565 MS.gene57991:CDS
TTGGTCGAGTCAAGGATTAT+GGG 0.449548 8.3:-10306532 MS.gene57991:CDS
AAGGATGAATTTGGGGTGGT+GGG 0.462409 8.3:-10306502 MS.gene57991:CDS
CTTCTTCGACGGCGCTGGAT+CGG 0.466202 8.3:-10306734 MS.gene57991:CDS
ATGTGAGGAATGTTTATGAT+TGG 0.470420 8.3:-10306444 MS.gene57991:CDS
ACGCAATCCCGACCAATCTT+CGG 0.484948 8.3:+10306577 None:intergenic
AAGGATTATGGGGAGGGTGA+AGG 0.488009 8.3:-10306521 MS.gene57991:CDS
GAGGAGTTCTTGAAGGAGAA+TGG 0.490553 8.3:-10306319 MS.gene57991:CDS
TATTCTCGAGGAGTTCTTGA+AGG 0.490607 8.3:-10306326 MS.gene57991:CDS
GTTCATCTTCATCGTAGAAT+CGG 0.510634 8.3:+10306680 None:intergenic
TGAACCGGGGAAGTTGGGGT+TGG 0.522977 8.3:-10306551 MS.gene57991:CDS
GAGGGTGAAGGATGAATTTG+GGG 0.527844 8.3:-10306509 MS.gene57991:CDS
GGTGAAGGATGAATTTGGGG+TGG 0.535313 8.3:-10306506 MS.gene57991:CDS
AGTTGGGGTTGGTCGAGTCA+AGG 0.540513 8.3:-10306540 MS.gene57991:CDS
TCGACCAACCCCAACTTCCC+CGG 0.541988 8.3:+10306547 None:intergenic
ATTTGGATCCGAAGATTGGT+CGG 0.545088 8.3:-10306585 MS.gene57991:CDS
TTGCTGAACCGGGGAAGTTG+GGG 0.545727 8.3:-10306555 MS.gene57991:CDS
TTTGGATCCGAAGATTGGTC+GGG 0.546401 8.3:-10306584 MS.gene57991:CDS
TCGTGTTGAAGAAGATGAGG+AGG 0.557685 8.3:-10306608 MS.gene57991:CDS
TGTTGTTTCGTTGAAAGCAA+AGG 0.565910 8.3:-10306653 MS.gene57991:CDS
TTTGTTTGAGATTGAATGTG+AGG 0.585638 8.3:-10306374 MS.gene57991:CDS
CGAGTCAAGGATTATGGGGA+GGG 0.587942 8.3:-10306527 MS.gene57991:CDS
AGGAATGTTTATGATTGGAA+AGG 0.602550 8.3:-10306439 MS.gene57991:CDS
CAACATAATCTCTTCTTCGA+CGG 0.610181 8.3:-10306745 MS.gene57991:CDS
CGATGAGTGATGACGTGGAA+AGG 0.614403 8.3:+10306769 None:intergenic
TTGTTTGAGATTGAATGTGA+GGG 0.624852 8.3:-10306373 MS.gene57991:CDS
GAGTCGTGTTGAAGAAGATG+AGG 0.643846 8.3:-10306611 MS.gene57991:CDS
CGTCGAAGAAGAGATTATGT+TGG 0.645098 8.3:+10306746 None:intergenic
TGTTTCGTTGAAAGCAAAGG+CGG 0.647467 8.3:-10306650 MS.gene57991:CDS
TGTGAGGGTTCCGATCCTGA+AGG 0.648829 8.3:-10306358 MS.gene57991:CDS
GGGATTGCGTTGCTGAACCG+GGG 0.653942 8.3:-10306564 MS.gene57991:CDS
AGGAGCTAAAAGTATTCTCG+AGG 0.663418 8.3:-10306338 MS.gene57991:CDS
TCGAGTCAAGGATTATGGGG+AGG 0.669429 8.3:-10306528 MS.gene57991:CDS
GAGAAAGCAAAGTAGTGACA+CGG 0.674575 8.3:+10306791 None:intergenic
GATTCTACGATGAAGATGAA+CGG 0.703862 8.3:-10306678 MS.gene57991:CDS
GTTGGCGATGAGTGATGACG+TGG 0.723488 8.3:+10306764 None:intergenic
TGGTCGAGTCAAGGATTATG+GGG 0.737226 8.3:-10306531 MS.gene57991:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATGAAACTCATTTTGATTT+TGG - chr8.3:10306693-10306712 MS.gene57991:CDS 25.0%
AGGAATGTTTATGATTGGAA+AGG - chr8.3:10306654-10306673 MS.gene57991:CDS 30.0%
ATGTGAGGAATGTTTATGAT+TGG - chr8.3:10306649-10306668 MS.gene57991:CDS 30.0%
! TGATTGGAAAGGTTTGAAAT+TGG - chr8.3:10306665-10306684 MS.gene57991:CDS 30.0%
! TTGTTTGAGATTGAATGTGA+GGG - chr8.3:10306720-10306739 MS.gene57991:CDS 30.0%
! TTTGTTTGAGATTGAATGTG+AGG - chr8.3:10306719-10306738 MS.gene57991:CDS 30.0%
CAACATAATCTCTTCTTCGA+CGG - chr8.3:10306348-10306367 MS.gene57991:CDS 35.0%
GATTCTACGATGAAGATGAA+CGG - chr8.3:10306415-10306434 MS.gene57991:CDS 35.0%
GTTCATCTTCATCGTAGAAT+CGG + chr8.3:10306416-10306435 None:intergenic 35.0%
!! TGTTGTTTCGTTGAAAGCAA+AGG - chr8.3:10306440-10306459 MS.gene57991:CDS 35.0%
!!! AAATTCGCAATTTTTCGAGC+TGG - chr8.3:10306825-10306844 MS.gene57991:CDS 35.0%
!!! AATTCGCAATTTTTCGAGCT+GGG - chr8.3:10306826-10306845 MS.gene57991:CDS 35.0%
!!! AGAATACTTTTAGCTCCTTC+AGG + chr8.3:10306753-10306772 None:intergenic 35.0%
ATTTGGATCCGAAGATTGGT+CGG - chr8.3:10306508-10306527 MS.gene57991:CDS 40.0%
CGTCGAAGAAGAGATTATGT+TGG + chr8.3:10306350-10306369 None:intergenic 40.0%
GAGAAAGCAAAGTAGTGACA+CGG + chr8.3:10306305-10306324 None:intergenic 40.0%
TATTCTCGAGGAGTTCTTGA+AGG - chr8.3:10306767-10306786 MS.gene57991:CDS 40.0%
TGAAGAAGATGAGGAGGATT+TGG - chr8.3:10306491-10306510 MS.gene57991:CDS 40.0%
TGTTTCGTTGAAAGCAAAGG+CGG - chr8.3:10306443-10306462 MS.gene57991:CDS 40.0%
TTGGTCGAGTCAAGGATTAT+GGG - chr8.3:10306561-10306580 MS.gene57991:CDS 40.0%
! GAAAATGGGTTTGTGGGTTT+GGG - chr8.3:10306615-10306634 MS.gene57991:CDS 40.0%
! TGAAAATGGGTTTGTGGGTT+TGG - chr8.3:10306614-10306633 MS.gene57991:CDS 40.0%
!! AGGAGCTAAAAGTATTCTCG+AGG - chr8.3:10306755-10306774 MS.gene57991:CDS 40.0%
!!! ACTTTTAGCTCCTTCAGGAT+CGG + chr8.3:10306748-10306767 None:intergenic 40.0%
!!! TGGGAGGTTTTAAGAATGTG+AGG - chr8.3:10306634-10306653 MS.gene57991:CDS 40.0%
GAGGAGTTCTTGAAGGAGAA+TGG - chr8.3:10306774-10306793 MS.gene57991:CDS 45.0%
GAGGATTTGGATCCGAAGAT+TGG - chr8.3:10306504-10306523 MS.gene57991:CDS 45.0%
GAGGGTGAAGGATGAATTTG+GGG - chr8.3:10306584-10306603 MS.gene57991:CDS 45.0%
GAGTCGTGTTGAAGAAGATG+AGG - chr8.3:10306482-10306501 MS.gene57991:CDS 45.0%
GGAGGGTGAAGGATGAATTT+GGG - chr8.3:10306583-10306602 MS.gene57991:CDS 45.0%
GTTGGTCGAGTCAAGGATTA+TGG - chr8.3:10306560-10306579 MS.gene57991:CDS 45.0%
TCGTGTTGAAGAAGATGAGG+AGG - chr8.3:10306485-10306504 MS.gene57991:CDS 45.0%
TGGTCGAGTCAAGGATTATG+GGG - chr8.3:10306562-10306581 MS.gene57991:CDS 45.0%
! AAGGATGAATTTGGGGTGGT+GGG - chr8.3:10306591-10306610 MS.gene57991:CDS 45.0%
! GTGGGTGAAAATGGGTTTGT+GGG - chr8.3:10306609-10306628 MS.gene57991:CDS 45.0%
! GTTGAAAGCAAAGGCGGTTT+TGG - chr8.3:10306449-10306468 MS.gene57991:CDS 45.0%
! TTTGGATCCGAAGATTGGTC+GGG - chr8.3:10306509-10306528 MS.gene57991:CDS 45.0%
AAGGATTATGGGGAGGGTGA+AGG - chr8.3:10306572-10306591 MS.gene57991:CDS 50.0%
ACGCAATCCCGACCAATCTT+CGG + chr8.3:10306519-10306538 None:intergenic 50.0%
CGAGTCAAGGATTATGGGGA+GGG - chr8.3:10306566-10306585 MS.gene57991:CDS 50.0%
GGGAGGGTGAAGGATGAATT+TGG - chr8.3:10306582-10306601 MS.gene57991:CDS 50.0%
GGTGAAGGATGAATTTGGGG+TGG - chr8.3:10306587-10306606 MS.gene57991:CDS 50.0%
TCGAGTCAAGGATTATGGGG+AGG - chr8.3:10306565-10306584 MS.gene57991:CDS 50.0%
! AATGGGTTTGTGGGTTTGGG+AGG - chr8.3:10306618-10306637 MS.gene57991:CDS 50.0%
! CGATGAGTGATGACGTGGAA+AGG + chr8.3:10306327-10306346 None:intergenic 50.0%
! GAAGGATGAATTTGGGGTGG+TGG - chr8.3:10306590-10306609 MS.gene57991:CDS 50.0%
! GGTGGGTGAAAATGGGTTTG+TGG - chr8.3:10306608-10306627 MS.gene57991:CDS 50.0%
!! TTGGGGTGGTGGGTGAAAAT+GGG - chr8.3:10306601-10306620 MS.gene57991:CDS 50.0%
!! TTTGGGGTGGTGGGTGAAAA+TGG - chr8.3:10306600-10306619 MS.gene57991:CDS 50.0%
!!! GCAAAGGCGGTTTTGGTTGA+TGG - chr8.3:10306456-10306475 MS.gene57991:CDS 50.0%
AATCTCTTCTTCGACGGCGC+TGG - chr8.3:10306354-10306373 MS.gene57991:CDS 55.0%
GTTGCTGAACCGGGGAAGTT+GGG - chr8.3:10306537-10306556 MS.gene57991:CDS 55.0%
TCGGGATTGCGTTGCTGAAC+CGG - chr8.3:10306527-10306546 MS.gene57991:CDS 55.0%
TGTGAGGGTTCCGATCCTGA+AGG - chr8.3:10306735-10306754 MS.gene57991:CDS 55.0%
TTGCTGAACCGGGGAAGTTG+GGG - chr8.3:10306538-10306557 MS.gene57991:CDS 55.0%
! GTTGGCGATGAGTGATGACG+TGG + chr8.3:10306332-10306351 None:intergenic 55.0%
!! AGTTGGGGTTGGTCGAGTCA+AGG - chr8.3:10306553-10306572 MS.gene57991:CDS 55.0%
CGGGATTGCGTTGCTGAACC+GGG - chr8.3:10306528-10306547 MS.gene57991:CDS 60.0%
CGTTGCTGAACCGGGGAAGT+TGG - chr8.3:10306536-10306555 MS.gene57991:CDS 60.0%
GGGATTGCGTTGCTGAACCG+GGG - chr8.3:10306529-10306548 MS.gene57991:CDS 60.0%
TCGACCAACCCCAACTTCCC+CGG + chr8.3:10306549-10306568 None:intergenic 60.0%
TGAACCGGGGAAGTTGGGGT+TGG - chr8.3:10306542-10306561 MS.gene57991:CDS 60.0%
! CTTCTTCGACGGCGCTGGAT+CGG - chr8.3:10306359-10306378 MS.gene57991:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 10306255 10306860 10306255 ID=MS.gene57991
chr8.3 mRNA 10306255 10306860 10306255 ID=MS.gene57991.t1;Parent=MS.gene57991
chr8.3 exon 10306255 10306860 10306255 ID=MS.gene57991.t1.exon1;Parent=MS.gene57991.t1
chr8.3 CDS 10306255 10306860 10306255 ID=cds.MS.gene57991.t1;Parent=MS.gene57991.t1
Gene Sequence

>MS.gene57991

ATGGAGGTTGAAGTCAAGCTTCGTCTCGCTAATGCAGATTCTCATCGCCGTGTCACTACTTTGCTTTCTCCTTTCCACGTCATCACTCATCGCCAACATAATCTCTTCTTCGACGGCGCTGGATCGGAATTATCTTCTCGTCGTGCTGTTCTTCGTCTCCGATTCTACGATGAAGATGAACGGTGTGTTGTTTCGTTGAAAGCAAAGGCGGTTTTGGTTGATGGTGTGAGTCGTGTTGAAGAAGATGAGGAGGATTTGGATCCGAAGATTGGTCGGGATTGCGTTGCTGAACCGGGGAAGTTGGGGTTGGTCGAGTCAAGGATTATGGGGAGGGTGAAGGATGAATTTGGGGTGGTGGGTGAAAATGGGTTTGTGGGTTTGGGAGGTTTTAAGAATGTGAGGAATGTTTATGATTGGAAAGGTTTGAAATTGGAAGTTGATGAAACTCATTTTGATTTTGGAACTTTGTTTGAGATTGAATGTGAGGGTTCCGATCCTGAAGGAGCTAAAAGTATTCTCGAGGAGTTCTTGAAGGAGAATGGAATTGATTATTCTTACTCTGTTGCTTCTAAATTCGCAATTTTTCGAGCTGGGAAATTGCCATAG

Protein sequence

>MS.gene57991.t1

MEVEVKLRLANADSHRRVTTLLSPFHVITHRQHNLFFDGAGSELSSRRAVLRLRFYDEDERCVVSLKAKAVLVDGVSRVEEDEEDLDPKIGRDCVAEPGKLGLVESRIMGRVKDEFGVVGENGFVGLGGFKNVRNVYDWKGLKLEVDETHFDFGTLFEIECEGSDPEGAKSILEEFLKENGIDYSYSVASKFAIFRAGKLP