Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60382.t1 | XP_013448123.1 | 81.2 | 64 | 12 | 0 | 20 | 83 | 121 | 184 | 5.90E-19 | 103.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60382.t1 | A0A072U8F6 | 81.2 | 64 | 12 | 0 | 20 | 83 | 121 | 184 | 4.2e-19 | 103.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene60382 | 0.83835 | 3.03E-57 | -1.69E-46 |
| MS.gene049254 | MS.gene60382 | 0.800194 | 1.62E-48 | -1.69E-46 |
| MS.gene049435 | MS.gene60382 | 0.805164 | 1.52E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene60382 | 0.804282 | 2.33E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene60382 | 0.801719 | 7.89E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene60382 | 0.805091 | 1.58E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene60382 | 0.808093 | 3.67E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene60382 | 0.811946 | 5.43E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene60382 | 0.801863 | 7.37E-49 | -1.69E-46 |
| MS.gene055623 | MS.gene60382 | 0.810848 | 9.40E-51 | -1.69E-46 |
| MS.gene055701 | MS.gene60382 | 0.803795 | 2.94E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene60382 | 0.818534 | 1.86E-52 | -1.69E-46 |
| MS.gene055926 | MS.gene60382 | 0.811123 | 8.19E-51 | -1.69E-46 |
| MS.gene05604 | MS.gene60382 | 0.81119 | 7.93E-51 | -1.69E-46 |
| MS.gene056174 | MS.gene60382 | 0.801526 | 8.64E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene60382 | 0.802398 | 5.72E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene60382 | 0.801456 | 8.93E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene60382 | 0.804977 | 1.67E-49 | -1.69E-46 |
| MS.gene05847 | MS.gene60382 | 0.803137 | 4.02E-49 | -1.69E-46 |
| MS.gene058548 | MS.gene60382 | 0.801358 | 9.35E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene60382 | 0.80937 | 1.96E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene60382 | 0.807831 | 4.17E-50 | -1.69E-46 |
| MS.gene060011 | MS.gene60382 | 0.800401 | 1.47E-48 | -1.69E-46 |
| MS.gene060310 | MS.gene60382 | 0.809583 | 1.76E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene60382 | 0.810686 | 1.02E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene60382 | 0.807499 | 4.91E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene60382 | 0.825174 | 5.41E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60382.t1 | MTR_7g033700 | 89.474 | 57 | 6 | 0 | 27 | 83 | 128 | 184 | 3.82e-29 | 106 |
| MS.gene60382.t1 | MTR_8g063090 | 80.702 | 57 | 11 | 0 | 27 | 83 | 126 | 182 | 5.67e-24 | 92.8 |
| MS.gene60382.t1 | MTR_7g033685 | 66.667 | 57 | 19 | 0 | 27 | 83 | 196 | 252 | 5.40e-17 | 73.9 |
| MS.gene60382.t1 | MTR_7g033685 | 72.549 | 51 | 14 | 0 | 33 | 83 | 8 | 58 | 1.51e-16 | 72.0 |
| MS.gene60382.t1 | MTR_7g033685 | 72.549 | 51 | 14 | 0 | 33 | 83 | 8 | 58 | 1.64e-16 | 71.6 |
| MS.gene60382.t1 | MTR_7g033685 | 72.549 | 51 | 14 | 0 | 33 | 83 | 122 | 172 | 1.94e-16 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGTTCTACATATATGATC+TGG | 0.374748 | 8.1:+23969682 | MS.gene60382:CDS |
| ACCCCTCTGTTCCTCTTCTC+CGG | 0.443592 | 8.1:-23969562 | None:intergenic |
| TTTACAGGTACAAACTCAAA+AGG | 0.451739 | 8.1:+23969654 | MS.gene60382:intron |
| CACGAACAATGTCATAGAAC+TGG | 0.474581 | 8.1:-23970649 | None:intergenic |
| CAGTTCTACATATATGATCT+GGG | 0.481346 | 8.1:+23969683 | MS.gene60382:CDS |
| ACATATATGATCTGGGGAAG+AGG | 0.507266 | 8.1:+23969690 | MS.gene60382:CDS |
| AAAAGATGCATCATACGATC+CGG | 0.564340 | 8.1:+23969543 | None:intergenic |
| ATCCGGAGAAGAGGAACAGA+GGG | 0.568562 | 8.1:+23969560 | MS.gene60382:CDS |
| ATCTGGGGAAGAGGAACAGA+GGG | 0.577768 | 8.1:+23969699 | MS.gene60382:CDS |
| CATCATACGATCCGGAGAAG+AGG | 0.594134 | 8.1:+23969551 | MS.gene60382:CDS |
| GAAGAGCAGAAAAGTAAACA+AGG | 0.626979 | 8.1:+23970592 | MS.gene60382:CDS |
| GATCTGGGGAAGAGGAACAG+AGG | 0.648910 | 8.1:+23969698 | MS.gene60382:CDS |
| AGAGCAGAAAAGTAAACAAG+GGG | 0.667224 | 8.1:+23970594 | MS.gene60382:CDS |
| AGTTCTACATATATGATCTG+GGG | 0.668433 | 8.1:+23969684 | MS.gene60382:CDS |
| AAGAGCAGAAAAGTAAACAA+GGG | 0.682340 | 8.1:+23970593 | MS.gene60382:CDS |
| GATCCGGAGAAGAGGAACAG+AGG | 0.702579 | 8.1:+23969559 | MS.gene60382:CDS |
| TCTGGGGAAGAGGAACAGAG+GGG | 0.757765 | 8.1:+23969700 | MS.gene60382:CDS |
| TCCGGAGAAGAGGAACAGAG+GGG | 0.782793 | 8.1:+23969561 | MS.gene60382:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAAAGAATAACTAAAAA+GGG | + | chr8.1:23970044-23970063 | MS.gene60382:intron | 15.0% |
| !! | GATAAAAAAAAAAAAAAAGG+AGG | + | chr8.1:23970087-23970106 | MS.gene60382:intron | 15.0% |
| !!! | TAAAAACTACAAAATTTGTT+TGG | + | chr8.1:23969954-23969973 | MS.gene60382:intron | 15.0% |
| !! | CAAAACTACTATTTGTAAAA+TGG | - | chr8.1:23969984-23970003 | None:intergenic | 20.0% |
| !! | GAAGAAAAGAATAACTAAAA+AGG | + | chr8.1:23970043-23970062 | MS.gene60382:intron | 20.0% |
| !! | TGTATCAATAATAATAAGCA+TGG | - | chr8.1:23970186-23970205 | None:intergenic | 20.0% |
| !!! | AATATTTTTTGAGAACAAAC+AGG | - | chr8.1:23969917-23969936 | None:intergenic | 20.0% |
| !!! | TTTTATGCTTTGATTTTTAC+AGG | + | chr8.1:23969639-23969658 | MS.gene60382:intron | 20.0% |
| ! | GTAACATACATTGAACTTTA+GGG | + | chr8.1:23970259-23970278 | MS.gene60382:intron | 25.0% |
| ! | TGTAACATACATTGAACTTT+AGG | + | chr8.1:23970258-23970277 | MS.gene60382:intron | 25.0% |
| !!! | AGTTGTTTTTGAAAAGAACT+TGG | - | chr8.1:23969798-23969817 | None:intergenic | 25.0% |
| !!! | GTTTATTTTATATCGACAGT+CGG | + | chr8.1:23970155-23970174 | MS.gene60382:intron | 25.0% |
| !!! | TTTATTTTATATCGACAGTC+GGG | + | chr8.1:23970156-23970175 | MS.gene60382:intron | 25.0% |
| AAGAGCAGAAAAGTAAACAA+GGG | + | chr8.1:23970593-23970612 | MS.gene60382:CDS | 30.0% | |
| AGTTCTACATATATGATCTG+GGG | + | chr8.1:23969684-23969703 | MS.gene60382:CDS | 30.0% | |
| CACTTAAATGAGAAGAAACT+TGG | - | chr8.1:23970310-23970329 | None:intergenic | 30.0% | |
| CAGTTCTACATATATGATCT+GGG | + | chr8.1:23969683-23969702 | MS.gene60382:CDS | 30.0% | |
| GTTCAATGTATGTTACACAT+TGG | - | chr8.1:23970255-23970274 | None:intergenic | 30.0% | |
| TCAGTTCTACATATATGATC+TGG | + | chr8.1:23969682-23969701 | MS.gene60382:CDS | 30.0% | |
| TTTACAGGTACAAACTCAAA+AGG | + | chr8.1:23969654-23969673 | MS.gene60382:intron | 30.0% | |
| ! | CTCTGAAGAAAAAGTTAATG+AGG | + | chr8.1:23970676-23970695 | MS.gene60382:CDS | 30.0% |
| !!! | AGTAGTTTTGAAAACCAGTT+TGG | + | chr8.1:23969994-23970013 | MS.gene60382:intron | 30.0% |
| !!! | GTAGTTTTGAAAACCAGTTT+GGG | + | chr8.1:23969995-23970014 | MS.gene60382:intron | 30.0% |
| AGAGCAGAAAAGTAAACAAG+GGG | + | chr8.1:23970594-23970613 | MS.gene60382:CDS | 35.0% | |
| CAAAACCTAAAAACCCAAAC+TGG | - | chr8.1:23970011-23970030 | None:intergenic | 35.0% | |
| GAAGAGCAGAAAAGTAAACA+AGG | + | chr8.1:23970592-23970611 | MS.gene60382:CDS | 35.0% | |
| TGATACTACCCATAATGAGA+CGG | + | chr8.1:23970455-23970474 | MS.gene60382:intron | 35.0% | |
| TTGTCAAGACAAAAACCCTT+GGG | + | chr8.1:23969891-23969910 | MS.gene60382:intron | 35.0% | |
| TTTGTCAAGACAAAAACCCT+TGG | + | chr8.1:23969890-23969909 | MS.gene60382:intron | 35.0% | |
| ! | AAAAAGGGTAGAGCAGTTTT+CGG | + | chr8.1:23970059-23970078 | MS.gene60382:intron | 35.0% |
| ! | AAGGGTTTTTGTCTTGACAA+AGG | - | chr8.1:23969891-23969910 | None:intergenic | 35.0% |
| ! | GAAGACTAAGTAGAGTATCA+AGG | - | chr8.1:23969769-23969788 | None:intergenic | 35.0% |
| !! | ATGCTCGTTTTGAGCAAATA+TGG | + | chr8.1:23970570-23970589 | MS.gene60382:CDS | 35.0% |
| !! | GAAAACCAGTTTGGGTTTTT+AGG | + | chr8.1:23970003-23970022 | MS.gene60382:intron | 35.0% |
| !!! | AGCGTTGTTACTTTTGCTAA+TGG | + | chr8.1:23970110-23970129 | MS.gene60382:intron | 35.0% |
| !!! | CAGTTTGGGTTTTTAGGTTT+TGG | + | chr8.1:23970009-23970028 | MS.gene60382:intron | 35.0% |
| !!! | GCGTTGTTACTTTTGCTAAT+GGG | + | chr8.1:23970111-23970130 | MS.gene60382:intron | 35.0% |
| ACATATATGATCTGGGGAAG+AGG | + | chr8.1:23969690-23969709 | MS.gene60382:CDS | 40.0% | |
| CACGAACAATGTCATAGAAC+TGG | - | chr8.1:23970652-23970671 | None:intergenic | 40.0% | |
| GAAGACTGCGAGAAAGAATA+AGG | - | chr8.1:23970542-23970561 | None:intergenic | 40.0% | |
| ACTACCCATAATGAGACGGA+GGG | + | chr8.1:23970459-23970478 | MS.gene60382:intron | 45.0% | |
| TACTACCCATAATGAGACGG+AGG | + | chr8.1:23970458-23970477 | MS.gene60382:intron | 45.0% | |
| TACTCCCTCCGTCTCATTAT+GGG | - | chr8.1:23970466-23970485 | None:intergenic | 45.0% | |
| TTACTCCCTCCGTCTCATTA+TGG | - | chr8.1:23970467-23970486 | None:intergenic | 45.0% | |
| TTGAGAACAAACAGGCCCAA+GGG | - | chr8.1:23969909-23969928 | None:intergenic | 45.0% | |
| TTTGAGAACAAACAGGCCCA+AGG | - | chr8.1:23969910-23969929 | None:intergenic | 45.0% | |
| !! | TTAGATAAAAAAAAAAAAAA+AGG | + | chr8.1:23970084-23970103 | MS.gene60382:intron | 5.0% |
| ATCCGGAGAAGAGGAACAGA+GGG | + | chr8.1:23969560-23969579 | MS.gene60382:CDS | 50.0% | |
| ATCTGGGGAAGAGGAACAGA+GGG | + | chr8.1:23969699-23969718 | MS.gene60382:CDS | 50.0% | |
| CATCATACGATCCGGAGAAG+AGG | + | chr8.1:23969551-23969570 | MS.gene60382:CDS | 50.0% | |
| ACCCCTCTGTTCCTCTTCTC+CGG | - | chr8.1:23969565-23969584 | None:intergenic | 55.0% | |
| GATCCGGAGAAGAGGAACAG+AGG | + | chr8.1:23969559-23969578 | MS.gene60382:CDS | 55.0% | |
| GATCTGGGGAAGAGGAACAG+AGG | + | chr8.1:23969698-23969717 | MS.gene60382:CDS | 55.0% | |
| TCCGGAGAAGAGGAACAGAG+GGG | + | chr8.1:23969561-23969580 | MS.gene60382:CDS | 55.0% | |
| TCTGGGGAAGAGGAACAGAG+GGG | + | chr8.1:23969700-23969719 | MS.gene60382:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 23969548 | 23970712 | 23969548 | ID=MS.gene60382 |
| chr8.1 | mRNA | 23969548 | 23970712 | 23969548 | ID=MS.gene60382.t1;Parent=MS.gene60382 |
| chr8.1 | exon | 23969548 | 23969582 | 23969548 | ID=MS.gene60382.t1.exon1;Parent=MS.gene60382.t1 |
| chr8.1 | CDS | 23969548 | 23969582 | 23969548 | ID=cds.MS.gene60382.t1;Parent=MS.gene60382.t1 |
| chr8.1 | exon | 23969661 | 23969721 | 23969661 | ID=MS.gene60382.t1.exon2;Parent=MS.gene60382.t1 |
| chr8.1 | CDS | 23969661 | 23969721 | 23969661 | ID=cds.MS.gene60382.t1;Parent=MS.gene60382.t1 |
| chr8.1 | exon | 23970557 | 23970712 | 23970557 | ID=MS.gene60382.t1.exon3;Parent=MS.gene60382.t1 |
| chr8.1 | CDS | 23970557 | 23970712 | 23970557 | ID=cds.MS.gene60382.t1;Parent=MS.gene60382.t1 |
| Gene Sequence |
| Protein sequence |
