Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61490.t1 | XP_003609286.1 | 95.1 | 102 | 2 | 1 | 1 | 99 | 1 | 102 | 2.20E-44 | 188 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61490.t1 | Q9SSC1 | 32.6 | 135 | 55 | 2 | 1 | 99 | 1 | 135 | 3.9e-10 | 65.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61490.t1 | G7JVC7 | 95.1 | 102 | 2 | 1 | 1 | 99 | 1 | 102 | 1.6e-44 | 188.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene61490 | 0.813998 | 1.93E-51 | -1.69E-46 |
| MS.gene049254 | MS.gene61490 | 0.808183 | 3.51E-50 | -1.69E-46 |
| MS.gene049499 | MS.gene61490 | 0.816068 | 6.69E-52 | -1.69E-46 |
| MS.gene049711 | MS.gene61490 | 0.832282 | 1.03E-55 | -1.69E-46 |
| MS.gene049807 | MS.gene61490 | 0.815314 | 9.85E-52 | -1.69E-46 |
| MS.gene050559 | MS.gene61490 | 0.80133 | 9.48E-49 | -1.69E-46 |
| MS.gene051201 | MS.gene61490 | 0.812766 | 3.59E-51 | -1.69E-46 |
| MS.gene052037 | MS.gene61490 | 0.825087 | 5.68E-54 | -1.69E-46 |
| MS.gene052239 | MS.gene61490 | -0.800108 | 1.68E-48 | -1.69E-46 |
| MS.gene052307 | MS.gene61490 | 0.812712 | 3.69E-51 | -1.69E-46 |
| MS.gene052463 | MS.gene61490 | 0.806544 | 7.81E-50 | -1.69E-46 |
| MS.gene052533 | MS.gene61490 | 0.84622 | 2.49E-59 | -1.69E-46 |
| MS.gene053272 | MS.gene61490 | -0.813446 | 2.55E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene61490 | 0.813038 | 3.13E-51 | -1.69E-46 |
| MS.gene054892 | MS.gene61490 | 0.810203 | 1.30E-50 | -1.69E-46 |
| MS.gene054893 | MS.gene61490 | 0.817041 | 4.05E-52 | -1.69E-46 |
| MS.gene056677 | MS.gene61490 | 0.818992 | 1.47E-52 | -1.69E-46 |
| MS.gene056699 | MS.gene61490 | 0.833983 | 3.90E-56 | -1.69E-46 |
| MS.gene05686 | MS.gene61490 | 0.836221 | 1.06E-56 | -1.69E-46 |
| MS.gene057102 | MS.gene61490 | 0.816858 | 4.45E-52 | -1.69E-46 |
| MS.gene058781 | MS.gene61490 | 0.800811 | 1.21E-48 | -1.69E-46 |
| MS.gene05952 | MS.gene61490 | 0.819723 | 1.00E-52 | -1.69E-46 |
| MS.gene059634 | MS.gene61490 | 0.80263 | 5.12E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene61490 | -0.820217 | 7.71E-53 | -1.69E-46 |
| MS.gene060935 | MS.gene61490 | 0.814412 | 1.56E-51 | -1.69E-46 |
| MS.gene060937 | MS.gene61490 | 0.820756 | 5.80E-53 | -1.69E-46 |
| MS.gene061100 | MS.gene61490 | 0.804377 | 2.22E-49 | -1.69E-46 |
| MS.gene061188 | MS.gene61490 | 0.837535 | 4.91E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61490.t1 | MTR_4g114050 | 95.098 | 102 | 2 | 1 | 1 | 99 | 1 | 102 | 5.64e-66 | 194 |
| MS.gene61490.t1 | MTR_4g011060 | 55.319 | 94 | 30 | 4 | 1 | 83 | 1 | 93 | 6.78e-24 | 88.2 |
| MS.gene61490.t1 | MTR_2g103380 | 40.496 | 121 | 46 | 4 | 1 | 98 | 1 | 118 | 3.37e-21 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61490.t1 | AT5G20100 | 47.312 | 93 | 33 | 4 | 4 | 81 | 3 | 94 | 1.74e-17 | 71.6 |
Find 21 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAACGTTGACCGATCGATA+TGG | 0.363606 | 8.3:+13333746 | None:intergenic |
| AAGGTTCATCAGGATTGGTT+TGG | 0.367840 | 8.3:-13333865 | MS.gene61490:CDS |
| TGGGATGATAAATTCTTGTC+TGG | 0.375061 | 8.3:-13333845 | MS.gene61490:CDS |
| AGGTTCATCAGGATTGGTTT+GGG | 0.391343 | 8.3:-13333864 | MS.gene61490:CDS |
| TTCAGGAGACAAGGTTCATC+AGG | 0.416255 | 8.3:-13333875 | MS.gene61490:CDS |
| AGAAATCATTAGCATCATTC+AGG | 0.437100 | 8.3:-13333892 | MS.gene61490:CDS |
| GAGACAAGGTTCATCAGGAT+TGG | 0.459463 | 8.3:-13333870 | MS.gene61490:CDS |
| AAACCACATGTTGAAACCTT+AGG | 0.466270 | 8.3:+13333698 | None:intergenic |
| TTGGAGAGAAGCAGATCAGC+TGG | 0.478654 | 8.3:-13333776 | MS.gene61490:CDS |
| TTTGTGGAAGAAGCAGCAAC+TGG | 0.482811 | 8.3:+13333656 | None:intergenic |
| GAACAAGAGAGTGCATCAAC+TGG | 0.483164 | 8.3:-13333803 | MS.gene61490:CDS |
| AATTGACCCACCTTCTCCTA+AGG | 0.490014 | 8.3:-13333714 | MS.gene61490:CDS |
| TCTTGATTTAGGATTATTTG+TGG | 0.511223 | 8.3:+13333640 | None:intergenic |
| TCTCCTAAGGTTTCAACATG+TGG | 0.535168 | 8.3:-13333701 | MS.gene61490:CDS |
| GTTGAAACCTTAGGAGAAGG+TGG | 0.589036 | 8.3:+13333707 | None:intergenic |
| CATGTTGAAACCTTAGGAGA+AGG | 0.634843 | 8.3:+13333704 | None:intergenic |
| GAAGGTGGGTCAATTGATGT+TGG | 0.642101 | 8.3:+13333722 | None:intergenic |
| TGGAGCAAGACCATATCGAT+CGG | 0.648883 | 8.3:-13333756 | MS.gene61490:CDS |
| TTGAAACCTTAGGAGAAGGT+GGG | 0.649865 | 8.3:+13333708 | None:intergenic |
| TTAGCATCATTCAGGAGACA+AGG | 0.658604 | 8.3:-13333884 | MS.gene61490:CDS |
| GAGTGCATCAACTGGCACGT+TGG | 0.684698 | 8.3:-13333795 | MS.gene61490:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTGATTTAGGATTATTTG+TGG | + | chr8.3:13333893-13333912 | None:intergenic | 25.0% |
| AGAAATCATTAGCATCATTC+AGG | - | chr8.3:13333638-13333657 | MS.gene61490:CDS | 30.0% | |
| ! | GGTCTTCTTTTTCTTGATTT+AGG | + | chr8.3:13333904-13333923 | None:intergenic | 30.0% |
| AAACCACATGTTGAAACCTT+AGG | + | chr8.3:13333835-13333854 | None:intergenic | 35.0% | |
| TGGGATGATAAATTCTTGTC+TGG | - | chr8.3:13333685-13333704 | MS.gene61490:CDS | 35.0% | |
| ! | GTTTCAACATGTGGTTTTTG+TGG | - | chr8.3:13333838-13333857 | MS.gene61490:CDS | 35.0% |
| !!! | TGTGGTTTTTGTGGTTTGTT+TGG | - | chr8.3:13333847-13333866 | MS.gene61490:CDS | 35.0% |
| CATGTTGAAACCTTAGGAGA+AGG | + | chr8.3:13333829-13333848 | None:intergenic | 40.0% | |
| TCTCCTAAGGTTTCAACATG+TGG | - | chr8.3:13333829-13333848 | MS.gene61490:CDS | 40.0% | |
| TTAGCATCATTCAGGAGACA+AGG | - | chr8.3:13333646-13333665 | MS.gene61490:CDS | 40.0% | |
| TTGAAACCTTAGGAGAAGGT+GGG | + | chr8.3:13333825-13333844 | None:intergenic | 40.0% | |
| !! | AAGGTTCATCAGGATTGGTT+TGG | - | chr8.3:13333665-13333684 | MS.gene61490:CDS | 40.0% |
| !! | AGGTTCATCAGGATTGGTTT+GGG | - | chr8.3:13333666-13333685 | MS.gene61490:CDS | 40.0% |
| AATTGACCCACCTTCTCCTA+AGG | - | chr8.3:13333816-13333835 | MS.gene61490:CDS | 45.0% | |
| GAAGGTGGGTCAATTGATGT+TGG | + | chr8.3:13333811-13333830 | None:intergenic | 45.0% | |
| GAGACAAGGTTCATCAGGAT+TGG | - | chr8.3:13333660-13333679 | MS.gene61490:CDS | 45.0% | |
| GTTGAAACCTTAGGAGAAGG+TGG | + | chr8.3:13333826-13333845 | None:intergenic | 45.0% | |
| TGGAGCAAGACCATATCGAT+CGG | - | chr8.3:13333774-13333793 | MS.gene61490:CDS | 45.0% | |
| TTCAGGAGACAAGGTTCATC+AGG | - | chr8.3:13333655-13333674 | MS.gene61490:CDS | 45.0% | |
| TTTGTGGAAGAAGCAGCAAC+TGG | + | chr8.3:13333877-13333896 | None:intergenic | 45.0% | |
| ! | GAACAAGAGAGTGCATCAAC+TGG | - | chr8.3:13333727-13333746 | MS.gene61490:CDS | 45.0% |
| GCAACGTTGACCGATCGATA+TGG | + | chr8.3:13333787-13333806 | None:intergenic | 50.0% | |
| TTGGAGAGAAGCAGATCAGC+TGG | - | chr8.3:13333754-13333773 | MS.gene61490:CDS | 50.0% | |
| GAGTGCATCAACTGGCACGT+TGG | - | chr8.3:13333735-13333754 | MS.gene61490:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 13333625 | 13333927 | 13333625 | ID=MS.gene61490 |
| chr8.3 | mRNA | 13333625 | 13333927 | 13333625 | ID=MS.gene61490.t1;Parent=MS.gene61490 |
| chr8.3 | exon | 13333625 | 13333927 | 13333625 | ID=MS.gene61490.t1.exon1;Parent=MS.gene61490.t1 |
| chr8.3 | CDS | 13333625 | 13333927 | 13333625 | ID=cds.MS.gene61490.t1;Parent=MS.gene61490.t1 |
| Gene Sequence |
| Protein sequence |
