Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62843.t1 | XP_024638557.1 | 88.5 | 252 | 5 | 2 | 1 | 228 | 204 | 455 | 8.70E-122 | 446.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62843.t1 | A0A396ICJ7 | 88.5 | 252 | 5 | 2 | 1 | 228 | 204 | 455 | 6.3e-122 | 446.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene62843 | 0.808261 | 3.38E-50 | -1.69E-46 |
| MS.gene049435 | MS.gene62843 | 0.824093 | 9.73E-54 | -1.69E-46 |
| MS.gene049850 | MS.gene62843 | 0.804882 | 1.75E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene62843 | 0.833515 | 5.10E-56 | -1.69E-46 |
| MS.gene050807 | MS.gene62843 | 0.820572 | 6.39E-53 | -1.69E-46 |
| MS.gene050808 | MS.gene62843 | 0.800413 | 1.46E-48 | -1.69E-46 |
| MS.gene051198 | MS.gene62843 | 0.81135 | 7.32E-51 | -1.69E-46 |
| MS.gene05157 | MS.gene62843 | 0.809121 | 2.21E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene62843 | 0.827878 | 1.23E-54 | -1.69E-46 |
| MS.gene051720 | MS.gene62843 | 0.830596 | 2.69E-55 | -1.69E-46 |
| MS.gene052020 | MS.gene62843 | 0.82904 | 6.43E-55 | -1.69E-46 |
| MS.gene052323 | MS.gene62843 | 0.822324 | 2.52E-53 | -1.69E-46 |
| MS.gene052611 | MS.gene62843 | 0.83135 | 1.76E-55 | -1.69E-46 |
| MS.gene052612 | MS.gene62843 | 0.803433 | 3.49E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene62843 | 0.825739 | 3.98E-54 | -1.69E-46 |
| MS.gene052863 | MS.gene62843 | 0.804722 | 1.88E-49 | -1.69E-46 |
| MS.gene053166 | MS.gene62843 | 0.802 | 6.90E-49 | -1.69E-46 |
| MS.gene053221 | MS.gene62843 | 0.811033 | 8.57E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene62843 | 0.814095 | 1.83E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene62843 | 0.807906 | 4.02E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene62843 | 0.81179 | 5.87E-51 | -1.69E-46 |
| MS.gene054801 | MS.gene62843 | 0.803892 | 2.81E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene62843 | 0.804285 | 2.32E-49 | -1.69E-46 |
| MS.gene055357 | MS.gene62843 | 0.801112 | 1.05E-48 | -1.69E-46 |
| MS.gene055466 | MS.gene62843 | 0.814243 | 1.70E-51 | -1.69E-46 |
| MS.gene055926 | MS.gene62843 | 0.805973 | 1.03E-49 | -1.69E-46 |
| MS.gene05604 | MS.gene62843 | 0.805066 | 1.60E-49 | -1.69E-46 |
| MS.gene056173 | MS.gene62843 | 0.856537 | 3.01E-62 | -1.69E-46 |
| MS.gene056553 | MS.gene62843 | 0.804559 | 2.04E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene62843 | 0.80623 | 9.10E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene62843 | 0.806685 | 7.30E-50 | -1.69E-46 |
| MS.gene059937 | MS.gene62843 | 0.808877 | 2.50E-50 | -1.69E-46 |
| MS.gene060137 | MS.gene62843 | -0.819457 | 1.15E-52 | -1.69E-46 |
| MS.gene060310 | MS.gene62843 | 0.802472 | 5.52E-49 | -1.69E-46 |
| MS.gene060587 | MS.gene62843 | 0.82447 | 7.93E-54 | -1.69E-46 |
| MS.gene060803 | MS.gene62843 | 0.80843 | 3.11E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAGGAATGCTAGAGAAAT+TGG | 0.288023 | 8.4:-22819712 | MS.gene62843:CDS |
| TGCTGACAAGTTCAAATCTA+AGG | 0.339805 | 8.4:-22820090 | MS.gene62843:CDS |
| TCAATTACACGTCTCAAATT+CGG | 0.370717 | 8.4:-22819920 | MS.gene62843:CDS |
| CGGTCACACATGGTTGCTTC+GGG | 0.373854 | 8.4:-22819865 | MS.gene62843:CDS |
| TTATGAAAATGATCAGAGTT+TGG | 0.374759 | 8.4:-22820268 | MS.gene62843:CDS |
| CTATACAAACTTGTCCCATT+TGG | 0.380205 | 8.4:+22820225 | None:intergenic |
| ACGGTCACACATGGTTGCTT+CGG | 0.388677 | 8.4:-22819866 | MS.gene62843:CDS |
| TTCTTTCAAATTGCTTCTGA+TGG | 0.392257 | 8.4:-22820306 | MS.gene62843:CDS |
| TCCTCAATCATTGCATAAAC+AGG | 0.393964 | 8.4:+22819804 | None:intergenic |
| TTCAAATTGCTTCTGATGGA+TGG | 0.399644 | 8.4:-22820302 | MS.gene62843:CDS |
| GGAGTTTGTTGGGTCAAGAT+TGG | 0.402126 | 8.4:-22820384 | MS.gene62843:CDS |
| GTAAGAAAAGCTCTAAACTT+TGG | 0.410545 | 8.4:+22820437 | None:intergenic |
| ACATGGAGAATCAGAATCAT+TGG | 0.423257 | 8.4:-22820058 | MS.gene62843:CDS |
| TCTTCCAATGCGGGAGTTTG+AGG | 0.444808 | 8.4:-22819841 | MS.gene62843:CDS |
| AATCTAGTTCAGAATTGTGT+TGG | 0.445074 | 8.4:-22820119 | MS.gene62843:intron |
| AGATGGTATCCATGGAAGAT+AGG | 0.448392 | 8.4:-22819729 | MS.gene62843:CDS |
| TTGACAGTGAATCTTCCAAA+TGG | 0.449405 | 8.4:-22820240 | MS.gene62843:CDS |
| TCTAGCATTCCTATCTTCCA+TGG | 0.471509 | 8.4:+22819720 | None:intergenic |
| ATCTAGTTCAGAATTGTGTT+GGG | 0.471787 | 8.4:-22820118 | MS.gene62843:intron |
| AATCTCTCTTGTCAGTATCA+AGG | 0.473208 | 8.4:-22820029 | MS.gene62843:CDS |
| GCTTCGGGTTCTTCCAATGC+GGG | 0.486782 | 8.4:-22819850 | MS.gene62843:CDS |
| ACATCCTCAGCATAATTTGA+AGG | 0.492140 | 8.4:+22820174 | None:intergenic |
| GTTCGGTCGCGCGTTGCAGT+TGG | 0.493993 | 8.4:-22819773 | MS.gene62843:CDS |
| GATTGAGGATACGTTGAGTT+CGG | 0.503966 | 8.4:-22819790 | MS.gene62843:CDS |
| TGACAGTGAATCTTCCAAAT+GGG | 0.509190 | 8.4:-22820239 | MS.gene62843:CDS |
| ACCTGTTTATGCAATGATTG+AGG | 0.531813 | 8.4:-22819805 | MS.gene62843:CDS |
| AAATCCTCAAACTCCCGCAT+TGG | 0.532883 | 8.4:+22819837 | None:intergenic |
| TCTTGACGAACCGTTCAAGA+TGG | 0.534809 | 8.4:-22819746 | MS.gene62843:CDS |
| AAATATCAATTGCAAGAGTA+CGG | 0.542887 | 8.4:-22819885 | MS.gene62843:CDS |
| GATTGGGTTTATGAATCTTG+TGG | 0.555580 | 8.4:-22820488 | MS.gene62843:CDS |
| ACCGTTCAAGATGGTATCCA+TGG | 0.558865 | 8.4:-22819737 | MS.gene62843:CDS |
| ATCTAAGGCATTGAGAAACA+TGG | 0.571358 | 8.4:-22820075 | MS.gene62843:CDS |
| TCAGACACTGTTCGAAACAA+AGG | 0.576452 | 8.4:+22819969 | None:intergenic |
| TGTTCCTTCAAATTATGCTG+AGG | 0.577746 | 8.4:-22820178 | MS.gene62843:CDS |
| CTTGACCCAACAAACTCCCT+AGG | 0.588286 | 8.4:+22820389 | None:intergenic |
| TCCATGGATACCATCTTGAA+CGG | 0.597722 | 8.4:+22819736 | None:intergenic |
| AGTGTCTGAGAATTGTATGA+AGG | 0.600022 | 8.4:-22819955 | MS.gene62843:CDS |
| TGCAAGAGTACGGTCACACA+TGG | 0.618761 | 8.4:-22819875 | MS.gene62843:CDS |
| TGCTTCGGGTTCTTCCAATG+CGG | 0.623184 | 8.4:-22819851 | MS.gene62843:CDS |
| GGAGAATCAGAATCATTGGA+TGG | 0.629128 | 8.4:-22820054 | MS.gene62843:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGGTTTTAATAGTTTAATCA+AGG | - | chr8.4:22820218-22820237 | MS.gene62843:CDS | 20.0% |
| !!! | TATTGATTTTTTAGCTGATT+GGG | - | chr8.4:22819723-22819742 | MS.gene62843:CDS | 20.0% |
| ! | AAATATCAATTGCAAGAGTA+CGG | - | chr8.4:22820342-22820361 | MS.gene62843:intron | 25.0% |
| ! | TTATGAAAATGATCAGAGTT+TGG | - | chr8.4:22819959-22819978 | MS.gene62843:CDS | 25.0% |
| !!! | CTATTGATTTTTTAGCTGAT+TGG | - | chr8.4:22819722-22819741 | MS.gene62843:CDS | 25.0% |
| TCAATTACACGTCTCAAATT+CGG | - | chr8.4:22820307-22820326 | MS.gene62843:CDS | 30.0% | |
| TTCTTTCAAATTGCTTCTGA+TGG | - | chr8.4:22819921-22819940 | MS.gene62843:CDS | 30.0% | |
| ! | AATCTAGTTCAGAATTGTGT+TGG | - | chr8.4:22820108-22820127 | MS.gene62843:CDS | 30.0% |
| ! | ATCTAGTTCAGAATTGTGTT+GGG | - | chr8.4:22820109-22820128 | MS.gene62843:CDS | 30.0% |
| !! | GTAAGAAAAGCTCTAAACTT+TGG | + | chr8.4:22819793-22819812 | None:intergenic | 30.0% |
| !!! | TTCTGTTTCTTTTTCGAGTT+TGG | - | chr8.4:22819762-22819781 | MS.gene62843:CDS | 30.0% |
| AATCTCTCTTGTCAGTATCA+AGG | - | chr8.4:22820198-22820217 | MS.gene62843:CDS | 35.0% | |
| ACATCCTCAGCATAATTTGA+AGG | + | chr8.4:22820056-22820075 | None:intergenic | 35.0% | |
| ACATGGAGAATCAGAATCAT+TGG | - | chr8.4:22820169-22820188 | MS.gene62843:intron | 35.0% | |
| ACCTGTTTATGCAATGATTG+AGG | - | chr8.4:22820422-22820441 | MS.gene62843:CDS | 35.0% | |
| AGACAATAACTGGCATTTGT+GGG | - | chr8.4:22820085-22820104 | MS.gene62843:CDS | 35.0% | |
| AGTGTCTGAGAATTGTATGA+AGG | - | chr8.4:22820272-22820291 | MS.gene62843:CDS | 35.0% | |
| ATGCCAAGTTTGAAGAAGTT+AGG | - | chr8.4:22819866-22819885 | MS.gene62843:CDS | 35.0% | |
| CAACCTAACTTCTTCAAACT+TGG | + | chr8.4:22819872-22819891 | None:intergenic | 35.0% | |
| CTATACAAACTTGTCCCATT+TGG | + | chr8.4:22820005-22820024 | None:intergenic | 35.0% | |
| GATAGGAATGCTAGAGAAAT+TGG | - | chr8.4:22820515-22820534 | MS.gene62843:CDS | 35.0% | |
| GATTGGGTTTATGAATCTTG+TGG | - | chr8.4:22819739-22819758 | MS.gene62843:CDS | 35.0% | |
| TCCTCAATCATTGCATAAAC+AGG | + | chr8.4:22820426-22820445 | None:intergenic | 35.0% | |
| TGACAGTGAATCTTCCAAAT+GGG | - | chr8.4:22819988-22820007 | MS.gene62843:CDS | 35.0% | |
| TGCTGACAAGTTCAAATCTA+AGG | - | chr8.4:22820137-22820156 | MS.gene62843:intron | 35.0% | |
| TGTTCCTTCAAATTATGCTG+AGG | - | chr8.4:22820049-22820068 | MS.gene62843:CDS | 35.0% | |
| TTCAAATTGCTTCTGATGGA+TGG | - | chr8.4:22819925-22819944 | MS.gene62843:CDS | 35.0% | |
| TTGACAGTGAATCTTCCAAA+TGG | - | chr8.4:22819987-22820006 | MS.gene62843:CDS | 35.0% | |
| ! | AGAGCTTTTCTTACACAAGT+TGG | - | chr8.4:22819799-22819818 | MS.gene62843:CDS | 35.0% |
| ! | TTTTTGTGGGAGACAATAAC+TGG | - | chr8.4:22820075-22820094 | MS.gene62843:CDS | 35.0% |
| !! | ATCTAAGGCATTGAGAAACA+TGG | - | chr8.4:22820152-22820171 | MS.gene62843:intron | 35.0% |
| !! | TATGCTGAGGATGTTTTTGT+GGG | - | chr8.4:22820062-22820081 | MS.gene62843:CDS | 35.0% |
| !! | TTATGCTGAGGATGTTTTTG+TGG | - | chr8.4:22820061-22820080 | MS.gene62843:CDS | 35.0% |
| !! | TTTTTCCTAGGGAGTTTGTT+GGG | - | chr8.4:22819833-22819852 | MS.gene62843:CDS | 35.0% |
| !!! | AGAAGAGCTGTTTTTGTGAA+TGG | - | chr8.4:22820023-22820042 | MS.gene62843:CDS | 35.0% |
| ACAAACTCCCTAGGAAAAAC+AGG | + | chr8.4:22819832-22819851 | None:intergenic | 40.0% | |
| AGATGGTATCCATGGAAGAT+AGG | - | chr8.4:22820498-22820517 | MS.gene62843:CDS | 40.0% | |
| GAGACAATAACTGGCATTTG+TGG | - | chr8.4:22820084-22820103 | MS.gene62843:CDS | 40.0% | |
| GATTGAGGATACGTTGAGTT+CGG | - | chr8.4:22820437-22820456 | MS.gene62843:CDS | 40.0% | |
| GGAGAATCAGAATCATTGGA+TGG | - | chr8.4:22820173-22820192 | MS.gene62843:CDS | 40.0% | |
| TCAGACACTGTTCGAAACAA+AGG | + | chr8.4:22820261-22820280 | None:intergenic | 40.0% | |
| TCCATGGATACCATCTTGAA+CGG | + | chr8.4:22820494-22820513 | None:intergenic | 40.0% | |
| TCTAGCATTCCTATCTTCCA+TGG | + | chr8.4:22820510-22820529 | None:intergenic | 40.0% | |
| ! | GTTTTTCCTAGGGAGTTTGT+TGG | - | chr8.4:22819832-22819851 | MS.gene62843:CDS | 40.0% |
| !! | GTTAGGTTGAATCAGAAGCA+AGG | - | chr8.4:22819883-22819902 | MS.gene62843:CDS | 40.0% |
| !! | TTTGCCACCTGTTTTTCCTA+GGG | - | chr8.4:22819822-22819841 | MS.gene62843:CDS | 40.0% |
| AAATCCTCAAACTCCCGCAT+TGG | + | chr8.4:22820393-22820412 | None:intergenic | 45.0% | |
| AACTCCCTAGGAAAAACAGG+TGG | + | chr8.4:22819829-22819848 | None:intergenic | 45.0% | |
| ACCGTTCAAGATGGTATCCA+TGG | - | chr8.4:22820490-22820509 | MS.gene62843:CDS | 45.0% | |
| TCTTGACGAACCGTTCAAGA+TGG | - | chr8.4:22820481-22820500 | MS.gene62843:CDS | 45.0% | |
| !! | GGAGTTTGTTGGGTCAAGAT+TGG | - | chr8.4:22819843-22819862 | MS.gene62843:CDS | 45.0% |
| !! | GTTTGCCACCTGTTTTTCCT+AGG | - | chr8.4:22819821-22819840 | MS.gene62843:CDS | 45.0% |
| ACGGTCACACATGGTTGCTT+CGG | - | chr8.4:22820361-22820380 | MS.gene62843:intron | 50.0% | |
| CTTGACCCAACAAACTCCCT+AGG | + | chr8.4:22819841-22819860 | None:intergenic | 50.0% | |
| TCTTCCAATGCGGGAGTTTG+AGG | - | chr8.4:22820386-22820405 | MS.gene62843:CDS | 50.0% | |
| ! | TGCAAGAGTACGGTCACACA+TGG | - | chr8.4:22820352-22820371 | MS.gene62843:intron | 50.0% |
| ! | TGCTTCGGGTTCTTCCAATG+CGG | - | chr8.4:22820376-22820395 | MS.gene62843:intron | 50.0% |
| CGGTCACACATGGTTGCTTC+GGG | - | chr8.4:22820362-22820381 | MS.gene62843:intron | 55.0% | |
| ! | GCTTCGGGTTCTTCCAATGC+GGG | - | chr8.4:22820377-22820396 | MS.gene62843:CDS | 55.0% |
| GTTCGGTCGCGCGTTGCAGT+TGG | - | chr8.4:22820454-22820473 | MS.gene62843:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 22819704 | 22820545 | 22819704 | ID=MS.gene62843 |
| chr8.4 | mRNA | 22819704 | 22820545 | 22819704 | ID=MS.gene62843.t1;Parent=MS.gene62843 |
| chr8.4 | exon | 22820377 | 22820545 | 22820377 | ID=MS.gene62843.t1.exon1;Parent=MS.gene62843.t1 |
| chr8.4 | CDS | 22820377 | 22820545 | 22820377 | ID=cds.MS.gene62843.t1;Parent=MS.gene62843.t1 |
| chr8.4 | exon | 22820170 | 22820344 | 22820170 | ID=MS.gene62843.t1.exon2;Parent=MS.gene62843.t1 |
| chr8.4 | CDS | 22820170 | 22820344 | 22820170 | ID=cds.MS.gene62843.t1;Parent=MS.gene62843.t1 |
| chr8.4 | exon | 22819704 | 22820129 | 22819704 | ID=MS.gene62843.t1.exon3;Parent=MS.gene62843.t1 |
| chr8.4 | CDS | 22819704 | 22820129 | 22819704 | ID=cds.MS.gene62843.t1;Parent=MS.gene62843.t1 |
| Gene Sequence |
| Protein sequence |
