Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63100.t1 | XP_024629447.1 | 73.3 | 675 | 13 | 2 | 1 | 508 | 1 | 675 | 1.70E-265 | 924.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63100.t1 | Q5UBY2 | 25.0 | 587 | 324 | 17 | 1 | 494 | 1 | 564 | 8.1e-35 | 149.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63100.t1 | A0A396GJD5 | 73.3 | 675 | 13 | 2 | 1 | 508 | 1 | 675 | 1.2e-265 | 924.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene63100.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049435 | MS.gene63100 | 0.817798 | 2.74E-52 | -1.69E-46 |
| MS.gene050226 | MS.gene63100 | 0.802006 | 6.89E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene63100 | 0.811456 | 6.94E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene63100 | 0.817083 | 3.96E-52 | -1.69E-46 |
| MS.gene050442 | MS.gene63100 | 0.820549 | 6.47E-53 | -1.69E-46 |
| MS.gene050719 | MS.gene63100 | 0.823824 | 1.13E-53 | -1.69E-46 |
| MS.gene050721 | MS.gene63100 | 0.819577 | 1.08E-52 | -1.69E-46 |
| MS.gene050724 | MS.gene63100 | 0.807734 | 4.37E-50 | -1.69E-46 |
| MS.gene051207 | MS.gene63100 | 0.808098 | 3.66E-50 | -1.69E-46 |
| MS.gene051247 | MS.gene63100 | 0.804259 | 2.35E-49 | -1.69E-46 |
| MS.gene052012 | MS.gene63100 | 0.810637 | 1.04E-50 | -1.69E-46 |
| MS.gene052190 | MS.gene63100 | 0.8156 | 8.51E-52 | -1.69E-46 |
| MS.gene052208 | MS.gene63100 | 0.824475 | 7.91E-54 | -1.69E-46 |
| MS.gene052333 | MS.gene63100 | 0.803264 | 3.79E-49 | -1.69E-46 |
| MS.gene052773 | MS.gene63100 | 0.80045 | 1.43E-48 | -1.69E-46 |
| MS.gene053221 | MS.gene63100 | 0.801557 | 8.51E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene63100 | 0.829308 | 5.54E-55 | -1.69E-46 |
| MS.gene05415 | MS.gene63100 | 0.803245 | 3.82E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene63100 | -0.810589 | 1.07E-50 | -1.69E-46 |
| MS.gene054467 | MS.gene63100 | 0.817052 | 4.03E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene63100 | 0.835441 | 1.68E-56 | -1.69E-46 |
| MS.gene054775 | MS.gene63100 | 0.800334 | 1.51E-48 | -1.69E-46 |
| MS.gene055356 | MS.gene63100 | 0.804133 | 2.50E-49 | -1.69E-46 |
| MS.gene055778 | MS.gene63100 | 0.803046 | 4.20E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene63100 | 0.810757 | 9.84E-51 | -1.69E-46 |
| MS.gene05604 | MS.gene63100 | 0.820431 | 6.89E-53 | -1.69E-46 |
| MS.gene056173 | MS.gene63100 | 0.816796 | 4.60E-52 | -1.69E-46 |
| MS.gene056498 | MS.gene63100 | 0.805776 | 1.13E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene63100 | 0.841305 | 5.15E-58 | -1.69E-46 |
| MS.gene057026 | MS.gene63100 | 0.850971 | 1.20E-60 | -1.69E-46 |
| MS.gene057915 | MS.gene63100 | 0.823602 | 1.27E-53 | -1.69E-46 |
| MS.gene058625 | MS.gene63100 | 0.811871 | 5.64E-51 | -1.69E-46 |
| MS.gene058636 | MS.gene63100 | 0.821402 | 4.12E-53 | -1.69E-46 |
| MS.gene058829 | MS.gene63100 | 0.804876 | 1.75E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene63100 | 0.814314 | 1.64E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene63100 | 0.808651 | 2.79E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene63100 | 0.819278 | 1.26E-52 | -1.69E-46 |
| MS.gene060683 | MS.gene63100 | 0.801704 | 7.94E-49 | -1.69E-46 |
| MS.gene060685 | MS.gene63100 | 0.805757 | 1.14E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene63100 | 0.810774 | 9.75E-51 | -1.69E-46 |
| MS.gene061040 | MS.gene63100 | -0.811543 | 6.64E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63100.t1 | MTR_6g051425 | 66.829 | 205 | 55 | 2 | 1 | 192 | 1 | 205 | 4.03e-92 | 285 |
| MS.gene63100.t1 | MTR_2g039370 | 78.873 | 71 | 15 | 0 | 49 | 119 | 98 | 168 | 1.92e-32 | 126 |
| MS.gene63100.t1 | MTR_3g465760 | 79.710 | 69 | 10 | 1 | 41 | 105 | 2 | 70 | 2.20e-31 | 115 |
| MS.gene63100.t1 | MTR_2g098920 | 30.088 | 113 | 75 | 2 | 12 | 124 | 78 | 186 | 5.05e-12 | 66.6 |
| MS.gene63100.t1 | MTR_0402s0040 | 26.697 | 221 | 124 | 11 | 29 | 239 | 38 | 230 | 5.71e-12 | 68.2 |
| MS.gene63100.t1 | MTR_4g035820 | 25.914 | 301 | 162 | 14 | 18 | 267 | 76 | 366 | 7.79e-12 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63100.t1 | AT2G27110 | 27.390 | 387 | 246 | 9 | 110 | 483 | 136 | 500 | 3.10e-36 | 143 |
| MS.gene63100.t1 | AT2G27110 | 29.043 | 303 | 194 | 6 | 186 | 483 | 359 | 645 | 3.84e-36 | 143 |
| MS.gene63100.t1 | AT2G27110 | 29.043 | 303 | 194 | 6 | 186 | 483 | 359 | 645 | 3.84e-36 | 143 |
| MS.gene63100.t1 | AT4G38180 | 31.839 | 223 | 149 | 3 | 194 | 415 | 420 | 640 | 2.58e-33 | 134 |
| MS.gene63100.t1 | AT4G38180 | 30.457 | 197 | 119 | 6 | 13 | 196 | 72 | 263 | 8.89e-14 | 74.7 |
| MS.gene63100.t1 | AT4G38170 | 27.039 | 233 | 168 | 2 | 186 | 418 | 177 | 407 | 9.68e-31 | 125 |
| MS.gene63100.t1 | AT4G19990 | 32.727 | 220 | 146 | 2 | 192 | 411 | 286 | 503 | 1.14e-28 | 120 |
| MS.gene63100.t1 | AT4G19990 | 32.727 | 220 | 146 | 2 | 192 | 411 | 343 | 560 | 1.32e-28 | 120 |
| MS.gene63100.t1 | AT4G19990 | 32.727 | 220 | 146 | 2 | 192 | 411 | 343 | 560 | 1.40e-28 | 120 |
| MS.gene63100.t1 | AT4G19990 | 32.727 | 220 | 146 | 2 | 192 | 411 | 343 | 560 | 1.40e-28 | 120 |
| MS.gene63100.t1 | AT4G19990 | 32.727 | 220 | 146 | 2 | 192 | 411 | 357 | 574 | 1.64e-28 | 120 |
| MS.gene63100.t1 | AT4G19990 | 32.727 | 220 | 146 | 2 | 192 | 411 | 343 | 560 | 1.97e-28 | 120 |
| MS.gene63100.t1 | AT4G19990 | 32.727 | 220 | 146 | 2 | 192 | 411 | 343 | 560 | 1.97e-28 | 120 |
| MS.gene63100.t1 | AT4G15090 | 30.932 | 236 | 156 | 4 | 195 | 430 | 397 | 625 | 6.78e-28 | 118 |
| MS.gene63100.t1 | AT4G15090 | 30.932 | 236 | 156 | 4 | 195 | 430 | 397 | 625 | 6.78e-28 | 118 |
| MS.gene63100.t1 | AT4G15090 | 30.932 | 236 | 156 | 4 | 195 | 430 | 397 | 625 | 6.78e-28 | 118 |
| MS.gene63100.t1 | AT4G15090 | 30.932 | 236 | 156 | 4 | 195 | 430 | 397 | 625 | 6.78e-28 | 118 |
| MS.gene63100.t1 | AT4G15090 | 30.932 | 236 | 156 | 4 | 195 | 430 | 397 | 625 | 6.78e-28 | 118 |
| MS.gene63100.t1 | AT3G22170 | 30.568 | 229 | 150 | 4 | 196 | 421 | 407 | 629 | 2.02e-27 | 117 |
| MS.gene63100.t1 | AT3G22170 | 30.568 | 229 | 150 | 4 | 196 | 421 | 407 | 629 | 2.02e-27 | 117 |
| MS.gene63100.t1 | AT1G76320 | 29.630 | 216 | 147 | 2 | 195 | 410 | 334 | 544 | 7.43e-26 | 112 |
| MS.gene63100.t1 | AT1G76320 | 29.630 | 216 | 147 | 2 | 195 | 410 | 334 | 544 | 7.43e-26 | 112 |
| MS.gene63100.t1 | AT1G76320 | 29.630 | 216 | 147 | 2 | 195 | 410 | 334 | 544 | 8.17e-26 | 112 |
| MS.gene63100.t1 | AT1G76320 | 29.630 | 216 | 147 | 2 | 195 | 410 | 334 | 544 | 8.64e-26 | 112 |
| MS.gene63100.t1 | AT3G06250 | 25.656 | 343 | 225 | 7 | 79 | 409 | 379 | 703 | 3.35e-25 | 110 |
| MS.gene63100.t1 | AT3G06250 | 25.656 | 343 | 225 | 7 | 79 | 409 | 379 | 703 | 3.35e-25 | 110 |
| MS.gene63100.t1 | AT3G06250 | 25.656 | 343 | 225 | 7 | 79 | 409 | 379 | 703 | 3.35e-25 | 110 |
| MS.gene63100.t1 | AT5G18960 | 31.556 | 225 | 147 | 4 | 198 | 421 | 521 | 739 | 4.10e-24 | 107 |
| MS.gene63100.t1 | AT5G18960 | 31.556 | 225 | 147 | 4 | 198 | 421 | 521 | 739 | 4.10e-24 | 107 |
| MS.gene63100.t1 | AT2G32250 | 23.810 | 231 | 169 | 3 | 181 | 409 | 286 | 511 | 2.65e-18 | 89.0 |
| MS.gene63100.t1 | AT2G32250 | 23.810 | 231 | 169 | 3 | 181 | 409 | 286 | 511 | 2.84e-18 | 88.6 |
| MS.gene63100.t1 | AT2G32250 | 23.810 | 231 | 169 | 3 | 181 | 409 | 327 | 552 | 2.91e-18 | 88.6 |
| MS.gene63100.t1 | AT2G32250 | 23.810 | 231 | 169 | 3 | 181 | 409 | 327 | 552 | 3.13e-18 | 88.6 |
| MS.gene63100.t1 | AT2G32250 | 23.810 | 231 | 169 | 3 | 181 | 409 | 327 | 552 | 3.13e-18 | 88.6 |
| MS.gene63100.t1 | AT2G32250 | 23.810 | 231 | 169 | 3 | 181 | 409 | 327 | 552 | 3.34e-18 | 88.6 |
| MS.gene63100.t1 | AT2G32250 | 23.810 | 231 | 169 | 3 | 181 | 409 | 327 | 552 | 3.34e-18 | 88.6 |
| MS.gene63100.t1 | AT2G32250 | 23.810 | 231 | 169 | 3 | 181 | 409 | 327 | 552 | 3.34e-18 | 88.6 |
| MS.gene63100.t1 | AT1G52520 | 27.354 | 223 | 152 | 4 | 198 | 412 | 420 | 640 | 8.86e-17 | 84.0 |
| MS.gene63100.t1 | AT5G28530 | 23.605 | 233 | 161 | 5 | 197 | 426 | 416 | 634 | 6.21e-15 | 78.2 |
| MS.gene63100.t1 | AT5G28530 | 27.624 | 181 | 114 | 4 | 18 | 193 | 56 | 224 | 3.33e-12 | 69.3 |
| MS.gene63100.t1 | AT5G28530 | 23.605 | 233 | 161 | 5 | 197 | 426 | 416 | 634 | 6.21e-15 | 78.2 |
| MS.gene63100.t1 | AT5G28530 | 27.624 | 181 | 114 | 4 | 18 | 193 | 56 | 224 | 3.33e-12 | 69.3 |
| MS.gene63100.t1 | AT5G28530 | 23.605 | 233 | 161 | 5 | 197 | 426 | 353 | 571 | 9.06e-15 | 77.4 |
| MS.gene63100.t1 | AT5G28530 | 28.743 | 167 | 106 | 3 | 18 | 179 | 56 | 214 | 3.04e-12 | 69.7 |
| MS.gene63100.t1 | AT5G28530 | 24.464 | 233 | 163 | 6 | 197 | 426 | 416 | 638 | 1.94e-14 | 76.6 |
| MS.gene63100.t1 | AT5G28530 | 27.624 | 181 | 114 | 4 | 18 | 193 | 56 | 224 | 3.15e-12 | 69.7 |
| MS.gene63100.t1 | AT5G28530 | 24.464 | 233 | 163 | 6 | 197 | 426 | 353 | 575 | 2.91e-14 | 75.9 |
| MS.gene63100.t1 | AT5G28530 | 28.743 | 167 | 106 | 3 | 18 | 179 | 56 | 214 | 2.90e-12 | 69.7 |
| MS.gene63100.t1 | AT1G10240 | 25.926 | 297 | 186 | 9 | 187 | 478 | 394 | 661 | 6.23e-14 | 75.1 |
| MS.gene63100.t1 | AT1G10240 | 29.630 | 189 | 115 | 6 | 18 | 198 | 49 | 227 | 4.46e-13 | 72.4 |
| MS.gene63100.t1 | AT1G10240 | 25.926 | 297 | 186 | 9 | 187 | 478 | 394 | 661 | 6.23e-14 | 75.1 |
| MS.gene63100.t1 | AT1G10240 | 29.630 | 189 | 115 | 6 | 18 | 198 | 49 | 227 | 4.46e-13 | 72.4 |
| MS.gene63100.t1 | AT1G80010 | 23.746 | 299 | 196 | 12 | 192 | 477 | 439 | 718 | 4.69e-12 | 68.9 |
| MS.gene63100.t1 | AT3G59470 | 31.092 | 119 | 75 | 3 | 2 | 120 | 61 | 172 | 4.01e-11 | 63.9 |
| MS.gene63100.t1 | AT3G59470 | 31.092 | 119 | 75 | 3 | 2 | 120 | 61 | 172 | 4.01e-11 | 63.9 |
| MS.gene63100.t1 | AT3G59470 | 31.092 | 119 | 75 | 3 | 2 | 120 | 73 | 184 | 4.76e-11 | 63.9 |
| MS.gene63100.t1 | AT4G12850 | 31.579 | 133 | 83 | 4 | 9 | 139 | 6 | 132 | 4.76e-11 | 61.2 |
Find 79 sgRNAs with CRISPR-Local
Find 129 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTACATCTGAATTTAAAT+TGG | 0.221172 | 8.1:+43035012 | None:intergenic |
| TTGCGGATTTATAACATTAA+AGG | 0.223732 | 8.1:-43035307 | MS.gene63100:CDS |
| CTTGTGCTTGTTTACTTTGT+AGG | 0.250825 | 8.1:+43036367 | None:intergenic |
| TGTGGAAATTACTTGATATT+GGG | 0.256264 | 8.1:-43035066 | MS.gene63100:CDS |
| ATGTGGAAATTACTTGATAT+TGG | 0.266059 | 8.1:-43035067 | MS.gene63100:CDS |
| GAAAGTTACCAAGTTGATAT+TGG | 0.311387 | 8.1:-43036619 | MS.gene63100:CDS |
| ACTACAATTAATCTTCATAT+TGG | 0.315911 | 8.1:+43035372 | None:intergenic |
| CAAGAGCATGCTTTCAAAAC+AGG | 0.323625 | 8.1:-43036834 | MS.gene63100:CDS |
| AACTTTCCACACTCCTTCCT+TGG | 0.367826 | 8.1:+43036635 | None:intergenic |
| TTGCCGCAAGTTTGATTCAA+TGG | 0.373385 | 8.1:-43035350 | MS.gene63100:CDS |
| AGAAGACCGCATAAAGTCTT+TGG | 0.373881 | 8.1:-43036523 | MS.gene63100:CDS |
| TTTCTAAATGAGTATCTTGA+AGG | 0.382168 | 8.1:-43035505 | MS.gene63100:CDS |
| TATAAGCAGTGCTCCACTTA+TGG | 0.383579 | 8.1:+43035785 | None:intergenic |
| ATCATCATTGATCACTGAAA+TGG | 0.387294 | 8.1:+43035180 | None:intergenic |
| GTCATTCTACCATCTAGTTT+CGG | 0.391713 | 8.1:-43035122 | MS.gene63100:CDS |
| GAGCCTAGAGATATTTATCT+TGG | 0.393295 | 8.1:-43036897 | MS.gene63100:CDS |
| CTAGGCTCATCAGTTTCAAT+TGG | 0.394540 | 8.1:+43036912 | None:intergenic |
| CTTGACTTTCCGAAACTAGA+TGG | 0.401068 | 8.1:+43035113 | None:intergenic |
| AAGAAGAATTTCAAAGAACT+TGG | 0.405034 | 8.1:-43035873 | MS.gene63100:CDS |
| TTATAAGCCACTTCACCATC+AGG | 0.415225 | 8.1:+43036708 | None:intergenic |
| TAAATATTAGTGAAACATTA+CGG | 0.422498 | 8.1:-43035567 | MS.gene63100:CDS |
| TGAGCACATGCCTAGGGGTA+TGG | 0.429091 | 8.1:-43035218 | MS.gene63100:CDS |
| TGTTTGTACTCTCAATCCTT+TGG | 0.442113 | 8.1:+43035728 | None:intergenic |
| TGCCGCAAGTTTGATTCAAT+GGG | 0.448344 | 8.1:-43035349 | MS.gene63100:CDS |
| TGTACTCTCAATCCTTTGGC+AGG | 0.451258 | 8.1:+43035732 | None:intergenic |
| TCCACCATTTGTAACTCTAA+TGG | 0.452852 | 8.1:+43036491 | None:intergenic |
| TTCTTTGTAGTCATGCTTTG+CGG | 0.453705 | 8.1:-43035324 | MS.gene63100:CDS |
| AATGAGTATCTTGAAGGAAC+AGG | 0.455830 | 8.1:-43035499 | MS.gene63100:CDS |
| CAATAAAGATTGAGCATCTC+CGG | 0.458631 | 8.1:+43036395 | None:intergenic |
| TAACAAGAGGAGGTGTACTT+TGG | 0.474858 | 8.1:+43035590 | None:intergenic |
| AACAAATATCTTTGCTGAAG+TGG | 0.482406 | 8.1:+43036576 | None:intergenic |
| TGTATGAAATTCGCCATAAG+TGG | 0.485780 | 8.1:-43035798 | MS.gene63100:CDS |
| AAGAACAAATTGCTTAGCTA+TGG | 0.488702 | 8.1:-43036673 | MS.gene63100:CDS |
| AGCAAGAATGTATTCTCTGC+TGG | 0.493952 | 8.1:-43035763 | MS.gene63100:CDS |
| AACATTACGGCATGCATCTA+CGG | 0.494329 | 8.1:-43035554 | MS.gene63100:CDS |
| GAGCACATGCCTAGGGGTAT+GGG | 0.504100 | 8.1:-43035217 | MS.gene63100:CDS |
| ATGCATAATTATGTCTCCAC+TGG | 0.517136 | 8.1:-43036438 | MS.gene63100:CDS |
| AAGTTGAAATTTATCGAAGC+CGG | 0.519905 | 8.1:-43036414 | MS.gene63100:CDS |
| TGATTTATGAGCACATGCCT+AGG | 0.520789 | 8.1:-43035225 | MS.gene63100:CDS |
| AAGAAGCTCAAAATATTATG+TGG | 0.525327 | 8.1:-43035084 | MS.gene63100:CDS |
| GACAAATGTCAATGAAAATG+AGG | 0.525546 | 8.1:-43034981 | MS.gene63100:CDS |
| GTGGCGCCGCATAGAGGTTG+AGG | 0.528015 | 8.1:-43035629 | MS.gene63100:CDS |
| TGACCAAGATAAATATCTCT+AGG | 0.529714 | 8.1:+43036894 | None:intergenic |
| AGATTTAATGTGACCAAGGA+AGG | 0.533919 | 8.1:-43036648 | MS.gene63100:CDS |
| AGAAGAATTTCAAAGAACTT+GGG | 0.538433 | 8.1:-43035872 | MS.gene63100:CDS |
| GGAAACATGCTTCAGACCAT+TGG | 0.541782 | 8.1:-43034960 | MS.gene63100:CDS |
| GATTTATGAGCACATGCCTA+GGG | 0.541821 | 8.1:-43035224 | MS.gene63100:CDS |
| GCCATTAGAGTTACAAATGG+TGG | 0.543834 | 8.1:-43036492 | MS.gene63100:CDS |
| ACTTGATATTGGGGCTGAGA+GGG | 0.547189 | 8.1:-43035056 | MS.gene63100:CDS |
| GATGCCATTAGAGTTACAAA+TGG | 0.552600 | 8.1:-43036495 | MS.gene63100:CDS |
| GTGGAAATTACTTGATATTG+GGG | 0.556011 | 8.1:-43035065 | MS.gene63100:CDS |
| TCACTAATATTTATAACAAG+AGG | 0.557518 | 8.1:+43035577 | None:intergenic |
| AGCATTGCGATAAAGAATAC+CGG | 0.559831 | 8.1:+43035153 | None:intergenic |
| AATATATCAATTCAGACCAA+TGG | 0.559963 | 8.1:+43034944 | None:intergenic |
| GTAAGCTTCAAGATCATCAA+TGG | 0.562706 | 8.1:-43035831 | MS.gene63100:CDS |
| GATAAATTTCAACTTTCCAG+TGG | 0.566800 | 8.1:+43036422 | None:intergenic |
| GAGAGGGTGCGAAAAGGTGT+TGG | 0.567841 | 8.1:-43035040 | MS.gene63100:CDS |
| GGGGCTGAGAGGGTGCGAAA+AGG | 0.580980 | 8.1:-43035046 | MS.gene63100:CDS |
| TACTTGATATTGGGGCTGAG+AGG | 0.584455 | 8.1:-43035057 | MS.gene63100:CDS |
| GAGTATCTTGAAGGAACAGG+TGG | 0.588038 | 8.1:-43035496 | MS.gene63100:CDS |
| GGTGCGAAAAGGTGTTGGCA+AGG | 0.590379 | 8.1:-43035035 | MS.gene63100:CDS |
| AAATTCAGATGTAGAAACAA+TGG | 0.596835 | 8.1:-43035005 | MS.gene63100:CDS |
| TGAGAGTACAAACAATGTGT+TGG | 0.597259 | 8.1:-43035719 | MS.gene63100:CDS |
| CTAATATTTATAACAAGAGG+AGG | 0.599669 | 8.1:+43035580 | None:intergenic |
| ATCATTGATCACTGAAATGG+AGG | 0.600594 | 8.1:+43035183 | None:intergenic |
| CATTCACCAAAGACTTTATG+CGG | 0.602476 | 8.1:+43036517 | None:intergenic |
| GAATCTTCTCCCATACCCCT+AGG | 0.605803 | 8.1:+43035208 | None:intergenic |
| TGCCCATTGAATCAAACTTG+CGG | 0.606774 | 8.1:+43035347 | None:intergenic |
| CAATGAACCTGATGGTGAAG+TGG | 0.607386 | 8.1:-43036715 | MS.gene63100:CDS |
| GTGATCAATGATGATGATGC+CGG | 0.629234 | 8.1:-43035172 | MS.gene63100:CDS |
| ATTGTGATCCAATATCAACT+TGG | 0.629329 | 8.1:+43036611 | None:intergenic |
| AGGCATGTGCTCATAAATCA+CGG | 0.631426 | 8.1:+43035228 | None:intergenic |
| CTGGTATCAAGTCCTGCCAA+AGG | 0.634306 | 8.1:-43035744 | MS.gene63100:CDS |
| ATGTGACCAAGGAAGGAGTG+TGG | 0.636883 | 8.1:-43036641 | MS.gene63100:CDS |
| GGTCAGATTTAATGTGACCA+AGG | 0.650278 | 8.1:-43036652 | MS.gene63100:CDS |
| GCGCCGCATAGAGGTTGAGG+AGG | 0.668856 | 8.1:-43035626 | MS.gene63100:CDS |
| ATTTATGAGCACATGCCTAG+GGG | 0.718468 | 8.1:-43035223 | MS.gene63100:CDS |
| ATTCCTCCTCAACCTCTATG+CGG | 0.727553 | 8.1:+43035623 | None:intergenic |
| AATGGTGGAGTTGACAAACA+TGG | 0.742831 | 8.1:-43036477 | MS.gene63100:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATATTAGTGAAACATTA+CGG | - | chr8.1:43036321-43036340 | MS.gene63100:intron | 15.0% |
| !! | AAACTTATTTAGTAAATGCA+TGG | - | chr8.1:43035977-43035996 | MS.gene63100:intron | 20.0% |
| !! | ACTACAATTAATCTTCATAT+TGG | + | chr8.1:43036519-43036538 | None:intergenic | 20.0% |
| !! | TAAGCCTAATAAAAAATATG+TGG | - | chr8.1:43036478-43036497 | MS.gene63100:CDS | 20.0% |
| !! | TCACTAATATTTATAACAAG+AGG | + | chr8.1:43036314-43036333 | None:intergenic | 20.0% |
| !! | TTCTACATCTGAATTTAAAT+TGG | + | chr8.1:43036879-43036898 | None:intergenic | 20.0% |
| !!! | ATTGTTTTTAAATCCTTGTT+TGG | + | chr8.1:43035157-43035176 | None:intergenic | 20.0% |
| !!! | CATATTTTTTATTAGGCTTA+TGG | + | chr8.1:43036478-43036497 | None:intergenic | 20.0% |
| ! | AAATTCAGATGTAGAAACAA+TGG | - | chr8.1:43036883-43036902 | MS.gene63100:CDS | 25.0% |
| ! | AAGAAGAATTTCAAAGAACT+TGG | - | chr8.1:43036015-43036034 | MS.gene63100:intron | 25.0% |
| ! | AAGAAGCTCAAAATATTATG+TGG | - | chr8.1:43036804-43036823 | MS.gene63100:CDS | 25.0% |
| ! | ACCAATACTTCTTAAAAAGA+TGG | - | chr8.1:43036621-43036640 | MS.gene63100:CDS | 25.0% |
| ! | AGAAGAATTTCAAAGAACTT+GGG | - | chr8.1:43036016-43036035 | MS.gene63100:intron | 25.0% |
| ! | ATGTGGAAATTACTTGATAT+TGG | - | chr8.1:43036821-43036840 | MS.gene63100:CDS | 25.0% |
| ! | CTAATATTTATAACAAGAGG+AGG | + | chr8.1:43036311-43036330 | None:intergenic | 25.0% |
| ! | TAGTTGATTATCATTGCTTT+GGG | - | chr8.1:43035622-43035641 | MS.gene63100:CDS | 25.0% |
| ! | TGTGGAAATTACTTGATATT+GGG | - | chr8.1:43036822-43036841 | MS.gene63100:CDS | 25.0% |
| ! | TTATTTAGTAAATGCATGGA+AGG | - | chr8.1:43035981-43036000 | MS.gene63100:intron | 25.0% |
| ! | TTCACTTTCATCAAATAACA+AGG | + | chr8.1:43035754-43035773 | None:intergenic | 25.0% |
| ! | TTGCGGATTTATAACATTAA+AGG | - | chr8.1:43036581-43036600 | MS.gene63100:CDS | 25.0% |
| ! | TTTAAAAACAATGAACCTGA+TGG | - | chr8.1:43035165-43035184 | MS.gene63100:CDS | 25.0% |
| ! | TTTCTAAATGAGTATCTTGA+AGG | - | chr8.1:43036383-43036402 | MS.gene63100:CDS | 25.0% |
| !!! | ACCATCTTTTTAAGAAGTAT+TGG | + | chr8.1:43036625-43036644 | None:intergenic | 25.0% |
| !!! | GTCACCACATATTTTTTATT+AGG | + | chr8.1:43036485-43036504 | None:intergenic | 25.0% |
| !!! | TAAAGATTGTTTTTGGTTGT+TGG | + | chr8.1:43035824-43035843 | None:intergenic | 25.0% |
| AAAGATGGTCAAAAAATGCA+AGG | - | chr8.1:43036636-43036655 | MS.gene63100:CDS | 30.0% | |
| AACAAATATCTTTGCTGAAG+TGG | + | chr8.1:43035315-43035334 | None:intergenic | 30.0% | |
| AAGAACAAATTGCTTAGCTA+TGG | - | chr8.1:43035215-43035234 | MS.gene63100:CDS | 30.0% | |
| AAGTTGAAATTTATCGAAGC+CGG | - | chr8.1:43035474-43035493 | MS.gene63100:CDS | 30.0% | |
| AGTTGATTATCATTGCTTTG+GGG | - | chr8.1:43035623-43035642 | MS.gene63100:CDS | 30.0% | |
| ATCATCATTGATCACTGAAA+TGG | + | chr8.1:43036711-43036730 | None:intergenic | 30.0% | |
| ATTGTGATCCAATATCAACT+TGG | + | chr8.1:43035280-43035299 | None:intergenic | 30.0% | |
| CATGTCATCGTTTATGTTTA+TGG | - | chr8.1:43035895-43035914 | MS.gene63100:intron | 30.0% | |
| GAAAAAGAAAACACGTTTGA+AGG | - | chr8.1:43035119-43035138 | MS.gene63100:CDS | 30.0% | |
| GACAAATGTCAATGAAAATG+AGG | - | chr8.1:43036907-43036926 | MS.gene63100:CDS | 30.0% | |
| GATAAATTTCAACTTTCCAG+TGG | + | chr8.1:43035469-43035488 | None:intergenic | 30.0% | |
| GTAGTTGATTATCATTGCTT+TGG | - | chr8.1:43035621-43035640 | MS.gene63100:CDS | 30.0% | |
| GTGGAAATTACTTGATATTG+GGG | - | chr8.1:43036823-43036842 | MS.gene63100:CDS | 30.0% | |
| TGACCAAGATAAATATCTCT+AGG | + | chr8.1:43034997-43035016 | None:intergenic | 30.0% | |
| TGATGATTTACACCAACAAA+GGG | + | chr8.1:43035705-43035724 | None:intergenic | 30.0% | |
| TTGAGAAGATGTTGAAAAAG+TGG | - | chr8.1:43036240-43036259 | MS.gene63100:intron | 30.0% | |
| ! | AAAGTGAAGTTTCTTTTGCT+TGG | - | chr8.1:43035766-43035785 | MS.gene63100:CDS | 30.0% |
| ! | GATTTTTATTGCTCCAAACA+AGG | - | chr8.1:43035141-43035160 | MS.gene63100:CDS | 30.0% |
| ! | TGAGCTGTAAAGATTGTTTT+TGG | + | chr8.1:43035831-43035850 | None:intergenic | 30.0% |
| !! | AAAGCATTTTTGGAGAGTAT+GGG | - | chr8.1:43035795-43035814 | MS.gene63100:CDS | 30.0% |
| !! | AACAGGTTTTAGTGTAAGAA+AGG | - | chr8.1:43035071-43035090 | MS.gene63100:CDS | 30.0% |
| !! | ACAGGTTTTAGTGTAAGAAA+GGG | - | chr8.1:43035072-43035091 | MS.gene63100:CDS | 30.0% |
| !! | GAAAGTTACCAAGTTGATAT+TGG | - | chr8.1:43035269-43035288 | MS.gene63100:CDS | 30.0% |
| !! | TTGGTTGTTCAAAGCATTTT+TGG | - | chr8.1:43035785-43035804 | MS.gene63100:CDS | 30.0% |
| !!! | CTTTTTAAGAAGTATTGGTC+AGG | + | chr8.1:43036620-43036639 | None:intergenic | 30.0% |
| AAGAATGCTCCTTCACATTT+GGG | - | chr8.1:43035927-43035946 | MS.gene63100:intron | 35.0% | |
| AATGAGTATCTTGAAGGAAC+AGG | - | chr8.1:43036389-43036408 | MS.gene63100:CDS | 35.0% | |
| AGATTTAATGTGACCAAGGA+AGG | - | chr8.1:43035240-43035259 | MS.gene63100:CDS | 35.0% | |
| AGCATTGCGATAAAGAATAC+CGG | + | chr8.1:43036738-43036757 | None:intergenic | 35.0% | |
| ATCATTGATCACTGAAATGG+AGG | + | chr8.1:43036708-43036727 | None:intergenic | 35.0% | |
| ATGATTTACACCAACAAAGG+GGG | + | chr8.1:43035703-43035722 | None:intergenic | 35.0% | |
| ATGCATAATTATGTCTCCAC+TGG | - | chr8.1:43035450-43035469 | MS.gene63100:CDS | 35.0% | |
| CAATAAAGATTGAGCATCTC+CGG | + | chr8.1:43035496-43035515 | None:intergenic | 35.0% | |
| CATAAACGATGACATGCATT+TGG | + | chr8.1:43035891-43035910 | None:intergenic | 35.0% | |
| CATTCACCAAAGACTTTATG+CGG | + | chr8.1:43035374-43035393 | None:intergenic | 35.0% | |
| CTGTACACTGTAATAGAACA+TGG | + | chr8.1:43035550-43035569 | None:intergenic | 35.0% | |
| CTTGTGCTTGTTTACTTTGT+AGG | + | chr8.1:43035524-43035543 | None:intergenic | 35.0% | |
| GAGCCTAGAGATATTTATCT+TGG | - | chr8.1:43034991-43035010 | MS.gene63100:CDS | 35.0% | |
| GATGATTTACACCAACAAAG+GGG | + | chr8.1:43035704-43035723 | None:intergenic | 35.0% | |
| GATGCCATTAGAGTTACAAA+TGG | - | chr8.1:43035393-43035412 | MS.gene63100:CDS | 35.0% | |
| GTAAGCTTCAAGATCATCAA+TGG | - | chr8.1:43036057-43036076 | MS.gene63100:intron | 35.0% | |
| GTGATGATTTACACCAACAA+AGG | + | chr8.1:43035706-43035725 | None:intergenic | 35.0% | |
| TAAGAATGCTCCTTCACATT+TGG | - | chr8.1:43035926-43035945 | MS.gene63100:intron | 35.0% | |
| TCCACCATTTGTAACTCTAA+TGG | + | chr8.1:43035400-43035419 | None:intergenic | 35.0% | |
| TGTATGAAATTCGCCATAAG+TGG | - | chr8.1:43036090-43036109 | MS.gene63100:intron | 35.0% | |
| TGTTTGTACTCTCAATCCTT+TGG | + | chr8.1:43036163-43036182 | None:intergenic | 35.0% | |
| TTCTTTGTAGTCATGCTTTG+CGG | - | chr8.1:43036564-43036583 | MS.gene63100:CDS | 35.0% | |
| TTGTTGGTGTAAATCATCAC+TGG | - | chr8.1:43035706-43035725 | MS.gene63100:CDS | 35.0% | |
| ! | GTCATTCTACCATCTAGTTT+CGG | - | chr8.1:43036766-43036785 | MS.gene63100:CDS | 35.0% |
| ! | TGAGAGTACAAACAATGTGT+TGG | - | chr8.1:43036169-43036188 | MS.gene63100:intron | 35.0% |
| ! | TTTTAGTGTAAGAAAGGGGA+AGG | - | chr8.1:43035077-43035096 | MS.gene63100:CDS | 35.0% |
| !! | CAAAGCATTTTTGGAGAGTA+TGG | - | chr8.1:43035794-43035813 | MS.gene63100:CDS | 35.0% |
| !! | CAGGTTTTAGTGTAAGAAAG+GGG | - | chr8.1:43035073-43035092 | MS.gene63100:CDS | 35.0% |
| AACATTACGGCATGCATCTA+CGG | - | chr8.1:43036334-43036353 | MS.gene63100:intron | 40.0% | |
| ACCATCTCTCCAAAAGACAT+TGG | + | chr8.1:43035595-43035614 | None:intergenic | 40.0% | |
| AGAAGACCGCATAAAGTCTT+TGG | - | chr8.1:43035365-43035384 | MS.gene63100:CDS | 40.0% | |
| AGCAAGAATGTATTCTCTGC+TGG | - | chr8.1:43036125-43036144 | MS.gene63100:intron | 40.0% | |
| AGGCATGTGCTCATAAATCA+CGG | + | chr8.1:43036663-43036682 | None:intergenic | 40.0% | |
| ATGAGAGAACCCAAATGTGA+AGG | + | chr8.1:43035939-43035958 | None:intergenic | 40.0% | |
| ATTTATGAGCACATGCCTAG+GGG | - | chr8.1:43036665-43036684 | MS.gene63100:CDS | 40.0% | |
| CACTGGCAAAATGTCATGTT+TGG | - | chr8.1:43035723-43035742 | MS.gene63100:CDS | 40.0% | |
| CATTGGTCAAACGTGATTCA+TGG | + | chr8.1:43035578-43035597 | None:intergenic | 40.0% | |
| CTAGGCTCATCAGTTTCAAT+TGG | + | chr8.1:43034979-43034998 | None:intergenic | 40.0% | |
| CTTGACTTTCCGAAACTAGA+TGG | + | chr8.1:43036778-43036797 | None:intergenic | 40.0% | |
| GATTTATGAGCACATGCCTA+GGG | - | chr8.1:43036664-43036683 | MS.gene63100:CDS | 40.0% | |
| GCCATTAGAGTTACAAATGG+TGG | - | chr8.1:43035396-43035415 | MS.gene63100:CDS | 40.0% | |
| GGTCAGATTTAATGTGACCA+AGG | - | chr8.1:43035236-43035255 | MS.gene63100:CDS | 40.0% | |
| TATAAGCAGTGCTCCACTTA+TGG | + | chr8.1:43036106-43036125 | None:intergenic | 40.0% | |
| TGATTTATGAGCACATGCCT+AGG | - | chr8.1:43036663-43036682 | MS.gene63100:CDS | 40.0% | |
| TGCCCATTGAATCAAACTTG+CGG | + | chr8.1:43036544-43036563 | None:intergenic | 40.0% | |
| TGCCGCAAGTTTGATTCAAT+GGG | - | chr8.1:43036539-43036558 | MS.gene63100:CDS | 40.0% | |
| TTATAAGCCACTTCACCATC+AGG | + | chr8.1:43035183-43035202 | None:intergenic | 40.0% | |
| TTGCCGCAAGTTTGATTCAA+TGG | - | chr8.1:43036538-43036557 | MS.gene63100:CDS | 40.0% | |
| ! | AATGGTGGAGTTGACAAACA+TGG | - | chr8.1:43035411-43035430 | MS.gene63100:CDS | 40.0% |
| ! | CAAGAGCATGCTTTCAAAAC+AGG | - | chr8.1:43035054-43035073 | MS.gene63100:CDS | 40.0% |
| ! | CACGTTTGACCAATGTCTTT+TGG | - | chr8.1:43035583-43035602 | MS.gene63100:CDS | 40.0% |
| ! | GTGATCAATGATGATGATGC+CGG | - | chr8.1:43036716-43036735 | MS.gene63100:CDS | 40.0% |
| ! | TAACAAGAGGAGGTGTACTT+TGG | + | chr8.1:43036301-43036320 | None:intergenic | 40.0% |
| !! | ACCAATGTCTTTTGGAGAGA+TGG | - | chr8.1:43035591-43035610 | MS.gene63100:CDS | 40.0% |
| !!! | CTGTTTTGAAAGCATGCTCT+TGG | + | chr8.1:43035056-43035075 | None:intergenic | 40.0% |
| AACTTTCCACACTCCTTCCT+TGG | + | chr8.1:43035256-43035275 | None:intergenic | 45.0% | |
| ACTTGATATTGGGGCTGAGA+GGG | - | chr8.1:43036832-43036851 | MS.gene63100:CDS | 45.0% | |
| ATTCCTCCTCAACCTCTATG+CGG | + | chr8.1:43036268-43036287 | None:intergenic | 45.0% | |
| CATGGCTAAAGCTATTGGAG+AGG | - | chr8.1:43035860-43035879 | MS.gene63100:CDS | 45.0% | |
| CCAGTCATGGCTAAAGCTAT+TGG | - | chr8.1:43035855-43035874 | MS.gene63100:CDS | 45.0% | |
| GAGTATCTTGAAGGAACAGG+TGG | - | chr8.1:43036392-43036411 | MS.gene63100:CDS | 45.0% | |
| GGAAACATGCTTCAGACCAT+TGG | - | chr8.1:43036928-43036947 | MS.gene63100:CDS | 45.0% | |
| TACAGCTCATCATCCAGTCA+TGG | - | chr8.1:43035842-43035861 | MS.gene63100:CDS | 45.0% | |
| TACTTGATATTGGGGCTGAG+AGG | - | chr8.1:43036831-43036850 | MS.gene63100:CDS | 45.0% | |
| TGTACTCTCAATCCTTTGGC+AGG | + | chr8.1:43036159-43036178 | None:intergenic | 45.0% | |
| ! | CAATGAACCTGATGGTGAAG+TGG | - | chr8.1:43035173-43035192 | MS.gene63100:CDS | 45.0% |
| !! | CCAATAGCTTTAGCCATGAC+TGG | + | chr8.1:43035858-43035877 | None:intergenic | 45.0% |
| !! | TTGATTTGTGCCCCCTTTGT+TGG | - | chr8.1:43035690-43035709 | MS.gene63100:CDS | 45.0% |
| ATGTGACCAAGGAAGGAGTG+TGG | - | chr8.1:43035247-43035266 | MS.gene63100:CDS | 50.0% | |
| CTGGTATCAAGTCCTGCCAA+AGG | - | chr8.1:43036144-43036163 | MS.gene63100:intron | 50.0% | |
| GAATCTTCTCCCATACCCCT+AGG | + | chr8.1:43036683-43036702 | None:intergenic | 50.0% | |
| GAAAAAGTGGCGCCGCATAG+AGG | - | chr8.1:43036253-43036272 | MS.gene63100:intron | 55.0% | |
| TGAGCACATGCCTAGGGGTA+TGG | - | chr8.1:43036670-43036689 | MS.gene63100:CDS | 55.0% | |
| ! | GAGAGGGTGCGAAAAGGTGT+TGG | - | chr8.1:43036848-43036867 | MS.gene63100:CDS | 55.0% |
| ! | GAGCACATGCCTAGGGGTAT+GGG | - | chr8.1:43036671-43036690 | MS.gene63100:CDS | 55.0% |
| !! | GGTGCGAAAAGGTGTTGGCA+AGG | - | chr8.1:43036853-43036872 | MS.gene63100:CDS | 55.0% |
| GCGCCGCATAGAGGTTGAGG+AGG | - | chr8.1:43036262-43036281 | MS.gene63100:intron | 65.0% | |
| GTGGCGCCGCATAGAGGTTG+AGG | - | chr8.1:43036259-43036278 | MS.gene63100:intron | 65.0% | |
| ! | GGGGCTGAGAGGGTGCGAAA+AGG | - | chr8.1:43036842-43036861 | MS.gene63100:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 43034955 | 43036955 | 43034955 | ID=MS.gene63100 |
| chr8.1 | mRNA | 43034955 | 43036955 | 43034955 | ID=MS.gene63100.t1;Parent=MS.gene63100 |
| chr8.1 | exon | 43036382 | 43036955 | 43036382 | ID=MS.gene63100.t1.exon1;Parent=MS.gene63100.t1 |
| chr8.1 | CDS | 43036382 | 43036955 | 43036382 | ID=cds.MS.gene63100.t1;Parent=MS.gene63100.t1 |
| chr8.1 | exon | 43034955 | 43035907 | 43034955 | ID=MS.gene63100.t1.exon2;Parent=MS.gene63100.t1 |
| chr8.1 | CDS | 43034955 | 43035907 | 43034955 | ID=cds.MS.gene63100.t1;Parent=MS.gene63100.t1 |
| Gene Sequence |
| Protein sequence |
