AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65498

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65498.t1 MTR_7g101050 81.520 579 73 6 1 548 1 576 0.0 935
MS.gene65498.t1 MTR_8g102050 84.058 552 64 3 19 548 2 551 0.0 934
MS.gene65498.t1 MTR_4g100920 49.632 544 215 11 12 548 55 546 7.09e-175 505
MS.gene65498.t1 MTR_8g011530 43.728 574 279 11 5 547 72 632 3.74e-156 460
MS.gene65498.t1 MTR_4g100920 45.455 440 181 11 12 444 55 442 8.63e-118 356
MS.gene65498.t1 MTR_1g014140 30.249 281 176 8 272 545 271 538 2.17e-26 113
MS.gene65498.t1 MTR_5g028360 29.893 281 177 9 272 545 319 586 2.13e-25 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65498.t1 AT5G56680 63.826 575 178 4 1 548 1 572 0.0 769
MS.gene65498.t1 AT1G70980 63.937 574 178 6 1 548 1 571 0.0 747
MS.gene65498.t1 AT4G17300 49.529 531 212 10 25 548 86 567 4.31e-173 501
MS.gene65498.t1 AT4G17300 49.529 531 212 10 25 548 106 587 6.56e-173 501
MS.gene65498.t1 AT3G07420 45.238 504 246 8 68 547 140 637 5.70e-148 439
MS.gene65498.t1 AT3G07420 42.298 383 191 8 68 426 140 516 7.95e-93 294
MS.gene65498.t1 AT1G68420 62.500 80 30 0 302 381 1 80 9.29e-30 112
MS.gene65498.t1 AT4G31180 32.384 281 170 9 272 545 288 555 1.69e-28 120
MS.gene65498.t1 AT4G31180 32.384 281 170 9 272 545 288 555 1.69e-28 120
MS.gene65498.t1 AT4G26870 30.769 286 160 10 272 541 262 525 3.67e-27 115
MS.gene65498.t1 AT5G38750 33.537 164 66 4 18 178 4 127 2.38e-16 76.3

Find 135 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAATATCCTTTGCCTAAA+AGG 0.177868 1.3:+25879030 MS.gene65498:CDS
CCAGGCATTTGGCTGAATTT+TGG 0.190908 1.3:+25879576 MS.gene65498:CDS
TCTTGAGGATATGCAATTTA+TGG 0.198036 1.3:+25879697 MS.gene65498:CDS
CTTAGACAACTTGTTCTTAC+TGG 0.233716 1.3:+25878894 MS.gene65498:CDS
TAGAATGGTTTGATACCTTT+TGG 0.274490 1.3:-25879941 None:intergenic
TTGCATCGATCGTTTGAAAA+TGG 0.283409 1.3:+25879739 MS.gene65498:CDS
AGGAATATCGGGCTGAAGTT+TGG 0.310492 1.3:-25879399 None:intergenic
AATTTGAGAAGGAAGTAAAA+TGG 0.311241 1.3:+25879840 MS.gene65498:CDS
CATCTCAAACTTCCTCATTC+TGG 0.318006 1.3:+25878939 MS.gene65498:CDS
TACCATTGATATGGCTTAAC+AGG 0.322913 1.3:-25880100 None:intergenic
TTCTCACACTTCCAGGCATT+TGG 0.323004 1.3:+25879565 MS.gene65498:CDS
GCCGTCTTCACGGCAGATTA+TGG 0.326140 1.3:-25878721 None:intergenic
TCCGTCCTTTCGAGGAATAT+CGG 0.331192 1.3:-25879411 None:intergenic
AAGTCTGCAAACGCGATTTC+AGG 0.337547 1.3:-25879608 None:intergenic
AACGATAACTTGAAGGTTAC+CGG 0.339980 1.3:-25878853 None:intergenic
TCCATCGTTCAATTCCAAAA+AGG 0.340581 1.3:-25878823 None:intergenic
CTTAGTAGTGTGTATACATT+TGG 0.341074 1.3:+25879521 MS.gene65498:CDS
TCTTATACTTCGTCTCTGTT+AGG 0.342134 1.3:-25879895 None:intergenic
ATGGCTGATAAGGTTGATAA+AGG 0.344858 1.3:+25879716 MS.gene65498:CDS
ATTTGAGAAGGAAGTAAAAT+GGG 0.355264 1.3:+25879841 MS.gene65498:CDS
CCAAAGGTTGGAGAGTTAAT+TGG 0.360690 1.3:+25880016 MS.gene65498:CDS
TACAGAAGCCGTGGAAATAC+TGG 0.367856 1.3:+25879793 MS.gene65498:CDS
CAGAGAATTCTCACACTTCC+AGG 0.368101 1.3:+25879558 MS.gene65498:CDS
AGCAGATATCGTGTTGGTTC+TGG 0.374309 1.3:-25879096 None:intergenic
TGAAATCGCGTTTGCAGACT+TGG 0.375982 1.3:+25879610 MS.gene65498:CDS
AGGTCAATGTACCATTGATA+TGG 0.386274 1.3:-25880109 None:intergenic
AAAGGCGAAAGCACCCTTAT+CGG 0.396068 1.3:-25878805 None:intergenic
TGCCGTGAAGACGGCGGATC+AGG 0.396893 1.3:+25878729 MS.gene65498:CDS
GGATATTTAGCAGGATCAAC+AGG 0.402646 1.3:-25879017 None:intergenic
AAACATGCTGGATTCGGTCT+TGG 0.405121 1.3:+25880151 MS.gene65498:CDS
TATGCAATTTATGGCTGATA+AGG 0.406440 1.3:+25879706 MS.gene65498:CDS
GCGGTCAGAGAATTCAGCTT+TGG 0.406711 1.3:-25878685 None:intergenic
AACTTGAAGGTTACCGGCAC+AGG 0.417890 1.3:-25878847 None:intergenic
CGGCAGATTATGGATTGAAT+CGG 0.418024 1.3:-25878711 None:intergenic
AGAGGGTTACCGTGGATAAC+TGG 0.418145 1.3:-25878647 None:intergenic
ATCGGAGGATGGGTGAAAAC+TGG 0.418683 1.3:+25878774 MS.gene65498:CDS
AACTGGAAGAAAAGCCGATA+AGG 0.420840 1.3:+25878791 MS.gene65498:CDS
CCGTCCTTTCGAGGAATATC+GGG 0.423115 1.3:-25879410 None:intergenic
TTAAGGAGAAGGGGATAAGA+AGG 0.425964 1.3:+25879276 MS.gene65498:CDS
CAATCAATAGTCGTGACAAT+TGG 0.426024 1.3:-25879197 None:intergenic
CCAATTAACTCTCCAACCTT+TGG 0.429345 1.3:-25880016 None:intergenic
CATCATAACGCTCCTCCCTT+TGG 0.431653 1.3:-25880045 None:intergenic
CGGTCAGAGAATTCAGCTTT+GGG 0.433838 1.3:-25878684 None:intergenic
TCCACCTTTCCGTCCTTTCG+AGG 0.434861 1.3:-25879419 None:intergenic
GGAACTTGTGTAGTTGTTGA+TGG 0.435212 1.3:+25878915 MS.gene65498:CDS
CCAAAATTCAGCCAAATGCC+TGG 0.449352 1.3:-25879576 None:intergenic
GATTCGGTCTTGGCTTTGAA+AGG 0.456155 1.3:+25880161 MS.gene65498:CDS
TCACTGTTCCATACCGCCTC+AGG 0.466762 1.3:-25880129 None:intergenic
TCCATAATCTGCCGTGAAGA+CGG 0.470318 1.3:+25878720 MS.gene65498:CDS
ACAGTGAAACATGCTGGATT+CGG 0.470528 1.3:+25880145 MS.gene65498:CDS
AAGCTTCAACGATAACTTGA+AGG 0.473651 1.3:-25878860 None:intergenic
AACCTGATCCGCCGTCTTCA+CGG 0.474312 1.3:-25878731 None:intergenic
CCTAGAGACAGTGGCTGCTA+TGG 0.475899 1.3:+25879982 MS.gene65498:CDS
TTTCATAAATCTGCTCTGCC+AGG 0.478444 1.3:-25880244 None:intergenic
ATTCCCTTCCCACGATATCC+TGG 0.485303 1.3:+25880226 MS.gene65498:CDS
GATCGAGCTTCGCGCTGAAG+AGG 0.487208 1.3:+25878980 MS.gene65498:CDS
TGTATATGTAAGTCGAATGA+AGG 0.488290 1.3:-25879774 None:intergenic
AATTCTCTGCTCGAAATGTC+GGG 0.488819 1.3:-25879544 None:intergenic
TTAGGCAAAGGATATTTAGC+AGG 0.488927 1.3:-25879026 None:intergenic
ATAGTCTATAATTATCCAAA+AGG 0.495051 1.3:+25879926 MS.gene65498:CDS
GCTGAAGAGGTTCTTCATGT+TGG 0.501465 1.3:+25878993 MS.gene65498:CDS
TACATTGACCTGAGGCGGTA+TGG 0.502024 1.3:+25880121 MS.gene65498:CDS
GGATAATTATAGACTATGAC+AGG 0.507099 1.3:-25879920 None:intergenic
CCATAGCAGCCACTGTCTCT+AGG 0.509076 1.3:-25879982 None:intergenic
GAAGCTGTTGTTAAGGAGAA+GGG 0.510646 1.3:+25879266 MS.gene65498:CDS
GAATTCTCTGCTCGAAATGT+CGG 0.511450 1.3:-25879545 None:intergenic
GACGGCGGATCAGGTTTAGC+AGG 0.513325 1.3:+25878738 MS.gene65498:CDS
TCATTGAGGCGCATGTAGAA+TGG 0.514521 1.3:-25879956 None:intergenic
TTCTGTTTAGCTCCAGAATG+AGG 0.517449 1.3:-25878951 None:intergenic
CTGGAAGAATCTGCGAAGAA+TGG 0.519997 1.3:+25879812 MS.gene65498:CDS
TTGAACGATGGAACCTGTGC+CGG 0.520799 1.3:+25878834 MS.gene65498:CDS
GGGAACCTCGTCGGAGGAGA+GGG 0.522118 1.3:-25878664 None:intergenic
AAGCTGTTGTTAAGGAGAAG+GGG 0.524459 1.3:+25879267 MS.gene65498:CDS
CGATGTTGAAGCTGTTGTTA+AGG 0.528387 1.3:+25879259 MS.gene65498:CDS
GCAGGCAAGAAAGCTAGAAT+CGG 0.528940 1.3:+25878756 MS.gene65498:CDS
TGAAGCTGTTGTTAAGGAGA+AGG 0.529298 1.3:+25879265 MS.gene65498:CDS
TGTCATTCAACAAGGACTAA+AGG 0.529353 1.3:+25880066 MS.gene65498:CDS
AATCGCGTTTGCAGACTTGG+AGG 0.529982 1.3:+25879613 MS.gene65498:CDS
ACTGGAAGAAAAGCCGATAA+GGG 0.531063 1.3:+25878792 MS.gene65498:CDS
GGCAGATTATGGATTGAATC+GGG 0.531948 1.3:-25878710 None:intergenic
TGGGAACCTCGTCGGAGGAG+AGG 0.533868 1.3:-25878665 None:intergenic
AGAAAGCTAGAATCGGAGGA+TGG 0.535348 1.3:+25878763 MS.gene65498:CDS
GATGTTCTTGTGCCAAAGGT+TGG 0.542344 1.3:+25880004 MS.gene65498:CDS
ACTATTGATTGCGAGGGTGC+TGG 0.546380 1.3:+25879209 MS.gene65498:CDS
AAGGTTGGAGAGTTAATTGG+TGG 0.550938 1.3:+25880019 MS.gene65498:CDS
AGATTCTTCCAGTATTTCCA+CGG 0.551909 1.3:-25879801 None:intergenic
TATGGATGTTCTTGTGCCAA+AGG 0.553956 1.3:+25880000 MS.gene65498:CDS
CCCGATATTCCTCGAAAGGA+CGG 0.554931 1.3:+25879410 MS.gene65498:CDS
AGTTAATTGGTGGAAGCCAA+AGG 0.559685 1.3:+25880029 MS.gene65498:CDS
TCTGCTCTGCCAGGATATCG+TGG 0.559764 1.3:-25880235 None:intergenic
ACACGATATCTGCTGTTGCT+CGG 0.565153 1.3:+25879105 MS.gene65498:CDS
TATGGAACAGTGAAACATGC+TGG 0.566811 1.3:+25880139 MS.gene65498:CDS
ATTGCGAGGGTGCTGGTGAA+AGG 0.569925 1.3:+25879216 MS.gene65498:CDS
TTCAGCTTTGGGAACCTCGT+CGG 0.571743 1.3:-25878673 None:intergenic
GAAAGCTAGAATCGGAGGAT+GGG 0.575623 1.3:+25878764 MS.gene65498:CDS
GTATATGTAAGTCGAATGAA+GGG 0.579832 1.3:-25879773 None:intergenic
GAATGAGATTAGTGCTGCTG+AGG 0.581419 1.3:+25879370 MS.gene65498:CDS
GGCAAGAAAGCTAGAATCGG+AGG 0.582938 1.3:+25878759 MS.gene65498:CDS
TGCCTGTTAAGCCATATCAA+TGG 0.583784 1.3:+25880098 MS.gene65498:CDS
CTGCCAGGATATCGTGGGAA+GGG 0.584465 1.3:-25880229 None:intergenic
AAATCACGCAAAACTTCAAG+AGG 0.593482 1.3:-25879059 None:intergenic
GTTACTTGACAACTGTCTTG+AGG 0.594479 1.3:+25879682 MS.gene65498:CDS
GTCACGACTATTGATTGCGA+GGG 0.597022 1.3:+25879203 MS.gene65498:CDS
TCCTCGAAAGGACGGAAAGG+TGG 0.597542 1.3:+25879418 MS.gene65498:CDS
GTTAATTGGTGGAAGCCAAA+GGG 0.599290 1.3:+25880030 MS.gene65498:CDS
GAATGGTCAGAAATTTGAGA+AGG 0.604552 1.3:+25879829 MS.gene65498:CDS
GGTCGGACGGTGGTGATGAG+AGG 0.605120 1.3:-25878626 None:intergenic
ACCGTGGATAACTGGTCGGA+CGG 0.609180 1.3:-25878639 None:intergenic
GAAGGAGATTAGTGCTGCTG+TGG 0.610064 1.3:+25879294 MS.gene65498:CDS
CGTTATGATGTCATTCAACA+AGG 0.613502 1.3:+25880058 MS.gene65498:CDS
TCAGCCCGATATTCCTCGAA+AGG 0.613632 1.3:+25879406 MS.gene65498:CDS
TTATGGATTGAATCGGGACG+CGG 0.617154 1.3:-25878704 None:intergenic
AGCTTTGGGAACCTCGTCGG+AGG 0.618057 1.3:-25878670 None:intergenic
TGTCACGACTATTGATTGCG+AGG 0.620740 1.3:+25879202 MS.gene65498:CDS
CTGCTCTGCCAGGATATCGT+GGG 0.624533 1.3:-25880234 None:intergenic
TTTGAGAAGGAAGTAAAATG+GGG 0.626158 1.3:+25879842 MS.gene65498:CDS
TCTGCCAGGATATCGTGGGA+AGG 0.640927 1.3:-25880230 None:intergenic
AATGGTACATTGACCTGAGG+CGG 0.645218 1.3:+25880116 MS.gene65498:CDS
ACTTACATATACAGAAGCCG+TGG 0.654722 1.3:+25879784 MS.gene65498:CDS
CTGTCTCTAGGTCATCATTG+AGG 0.656876 1.3:-25879970 None:intergenic
ATAATCTGCCGTGAAGACGG+CGG 0.657362 1.3:+25878723 MS.gene65498:CDS
TATTCCTCGAAAGGACGGAA+AGG 0.658806 1.3:+25879415 MS.gene65498:CDS
ATCAATGGTACATTGACCTG+AGG 0.661230 1.3:+25880113 MS.gene65498:CDS
GTGGATAACTGGTCGGACGG+TGG 0.662379 1.3:-25878636 None:intergenic
AGCAACAGCAGATATCGTGT+TGG 0.663258 1.3:-25879102 None:intergenic
CAATGATGACCTAGAGACAG+TGG 0.666339 1.3:+25879973 MS.gene65498:CDS
ATATGTAAGTCGAATGAAGG+GGG 0.666943 1.3:-25879771 None:intergenic
ACCGTCCGACCAGTTATCCA+CGG 0.669853 1.3:+25878638 MS.gene65498:CDS
AATTGGTGGAAGCCAAAGGG+AGG 0.678168 1.3:+25880033 MS.gene65498:CDS
GTCGGAGGAGAGGGTTACCG+TGG 0.682551 1.3:-25878655 None:intergenic
TATATGTAAGTCGAATGAAG+GGG 0.702544 1.3:-25879772 None:intergenic
GGTTACCGTGGATAACTGGT+CGG 0.706814 1.3:-25878643 None:intergenic
GGTAACCCTCTCCTCCGACG+AGG 0.713572 1.3:+25878659 MS.gene65498:CDS
AATCACGCAAAACTTCAAGA+GGG 0.718553 1.3:-25879058 None:intergenic
GGATGATATGAAGTGCGCTG+AGG 0.752084 1.3:+25879634 MS.gene65498:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTCTATAATTATCCAAA+AGG + chr1.3:25879926-25879945 MS.gene65498:CDS 20.0%
! AATTTGAGAAGGAAGTAAAA+TGG + chr1.3:25879840-25879859 MS.gene65498:CDS 25.0%
! ATTTGAGAAGGAAGTAAAAT+GGG + chr1.3:25879841-25879860 MS.gene65498:CDS 25.0%
CTAAATATCCTTTGCCTAAA+AGG + chr1.3:25879030-25879049 MS.gene65498:CDS 30.0%
CTTAGTAGTGTGTATACATT+TGG + chr1.3:25879521-25879540 MS.gene65498:CDS 30.0%
GGATAATTATAGACTATGAC+AGG - chr1.3:25879923-25879942 None:intergenic 30.0%
GTATATGTAAGTCGAATGAA+GGG - chr1.3:25879776-25879795 None:intergenic 30.0%
GTGGATGAAATTAAAAAAGC+TGG + chr1.3:25879313-25879332 MS.gene65498:CDS 30.0%
TATATGTAAGTCGAATGAAG+GGG - chr1.3:25879775-25879794 None:intergenic 30.0%
TATGCAATTTATGGCTGATA+AGG + chr1.3:25879706-25879725 MS.gene65498:CDS 30.0%
TCTTGAGGATATGCAATTTA+TGG + chr1.3:25879697-25879716 MS.gene65498:CDS 30.0%
TGTATATGTAAGTCGAATGA+AGG - chr1.3:25879777-25879796 None:intergenic 30.0%
TTTGAGAAGGAAGTAAAATG+GGG + chr1.3:25879842-25879861 MS.gene65498:CDS 30.0%
! ATGTCAAATTTTTGTGTCAG+TGG + chr1.3:25879660-25879679 MS.gene65498:CDS 30.0%
! TAGAATGGTTTGATACCTTT+TGG - chr1.3:25879944-25879963 None:intergenic 30.0%
!! CATACATTTTTCAACAAGCA+TGG + chr1.3:25879155-25879174 MS.gene65498:CDS 30.0%
AAATCACGCAAAACTTCAAG+AGG - chr1.3:25879062-25879081 None:intergenic 35.0%
AACGATAACTTGAAGGTTAC+CGG - chr1.3:25878856-25878875 None:intergenic 35.0%
AAGCTTCAACGATAACTTGA+AGG - chr1.3:25878863-25878882 None:intergenic 35.0%
AATCACGCAAAACTTCAAGA+GGG - chr1.3:25879061-25879080 None:intergenic 35.0%
AGATTCTTCCAGTATTTCCA+CGG - chr1.3:25879804-25879823 None:intergenic 35.0%
AGGTCAATGTACCATTGATA+TGG - chr1.3:25880112-25880131 None:intergenic 35.0%
ATATGTAAGTCGAATGAAGG+GGG - chr1.3:25879774-25879793 None:intergenic 35.0%
CAATCAATAGTCGTGACAAT+TGG - chr1.3:25879200-25879219 None:intergenic 35.0%
CGTTATGATGTCATTCAACA+AGG + chr1.3:25880058-25880077 MS.gene65498:CDS 35.0%
GAATGGTCAGAAATTTGAGA+AGG + chr1.3:25879829-25879848 MS.gene65498:CDS 35.0%
TACCATTGATATGGCTTAAC+AGG - chr1.3:25880103-25880122 None:intergenic 35.0%
TCCATCGTTCAATTCCAAAA+AGG - chr1.3:25878826-25878845 None:intergenic 35.0%
TCTTATACTTCGTCTCTGTT+AGG - chr1.3:25879898-25879917 None:intergenic 35.0%
TGTCATTCAACAAGGACTAA+AGG + chr1.3:25880066-25880085 MS.gene65498:CDS 35.0%
TTAGGCAAAGGATATTTAGC+AGG - chr1.3:25879029-25879048 None:intergenic 35.0%
! AGGATGATTCTTTTTGCTAC+CGG + chr1.3:25880181-25880200 MS.gene65498:CDS 35.0%
! CTTAGACAACTTGTTCTTAC+TGG + chr1.3:25878894-25878913 MS.gene65498:CDS 35.0%
! TCACATCTCGAATATTTTCC+AGG - chr1.3:25880207-25880226 None:intergenic 35.0%
! TTGCATCGATCGTTTGAAAA+TGG + chr1.3:25879739-25879758 MS.gene65498:CDS 35.0%
!! ATGGCTGATAAGGTTGATAA+AGG + chr1.3:25879716-25879735 MS.gene65498:CDS 35.0%
!! CAAGCATTTTTGACTGTTTC+TGG + chr1.3:25879467-25879486 MS.gene65498:CDS 35.0%
!! CTCATTCTTATCTGCTTTTG+CGG - chr1.3:25879357-25879376 None:intergenic 35.0%
AACTGGAAGAAAAGCCGATA+AGG + chr1.3:25878791-25878810 MS.gene65498:CDS 40.0%
AAGCTGTTGTTAAGGAGAAG+GGG + chr1.3:25879267-25879286 MS.gene65498:CDS 40.0%
AAGGTTGGAGAGTTAATTGG+TGG + chr1.3:25880019-25880038 MS.gene65498:CDS 40.0%
AATTCTCTGCTCGAAATGTC+GGG - chr1.3:25879547-25879566 None:intergenic 40.0%
ACAGTGAAACATGCTGGATT+CGG + chr1.3:25880145-25880164 MS.gene65498:CDS 40.0%
ACTGGAAGAAAAGCCGATAA+GGG + chr1.3:25878792-25878811 MS.gene65498:CDS 40.0%
ACTTACATATACAGAAGCCG+TGG + chr1.3:25879784-25879803 MS.gene65498:CDS 40.0%
AGTTAATTGGTGGAAGCCAA+AGG + chr1.3:25880029-25880048 MS.gene65498:CDS 40.0%
ATCAATGGTACATTGACCTG+AGG + chr1.3:25880113-25880132 MS.gene65498:CDS 40.0%
CATCTCAAACTTCCTCATTC+TGG + chr1.3:25878939-25878958 MS.gene65498:CDS 40.0%
CCAAAGGTTGGAGAGTTAAT+TGG + chr1.3:25880016-25880035 MS.gene65498:CDS 40.0%
CCAATTAACTCTCCAACCTT+TGG - chr1.3:25880019-25880038 None:intergenic 40.0%
CGATGTTGAAGCTGTTGTTA+AGG + chr1.3:25879259-25879278 MS.gene65498:CDS 40.0%
GAAGCTGTTGTTAAGGAGAA+GGG + chr1.3:25879266-25879285 MS.gene65498:CDS 40.0%
GAATTCTCTGCTCGAAATGT+CGG - chr1.3:25879548-25879567 None:intergenic 40.0%
GGAACTTGTGTAGTTGTTGA+TGG + chr1.3:25878915-25878934 MS.gene65498:CDS 40.0%
GGATATTTAGCAGGATCAAC+AGG - chr1.3:25879020-25879039 None:intergenic 40.0%
GTTAATTGGTGGAAGCCAAA+GGG + chr1.3:25880030-25880049 MS.gene65498:CDS 40.0%
GTTACTTGACAACTGTCTTG+AGG + chr1.3:25879682-25879701 MS.gene65498:CDS 40.0%
TATGGAACAGTGAAACATGC+TGG + chr1.3:25880139-25880158 MS.gene65498:CDS 40.0%
TATGGATGTTCTTGTGCCAA+AGG + chr1.3:25880000-25880019 MS.gene65498:CDS 40.0%
TGAAGCTGTTGTTAAGGAGA+AGG + chr1.3:25879265-25879284 MS.gene65498:CDS 40.0%
TGCCTGTTAAGCCATATCAA+TGG + chr1.3:25880098-25880117 MS.gene65498:CDS 40.0%
TTAAGGAGAAGGGGATAAGA+AGG + chr1.3:25879276-25879295 MS.gene65498:CDS 40.0%
! ATCTCGAATATTTTCCAGGC+CGG - chr1.3:25880203-25880222 None:intergenic 40.0%
! CGGCAGATTATGGATTGAAT+CGG - chr1.3:25878714-25878733 None:intergenic 40.0%
! GCATTTGGCTGAATTTTGGA+TGG + chr1.3:25879580-25879599 MS.gene65498:CDS 40.0%
! GGCAGATTATGGATTGAATC+GGG - chr1.3:25878713-25878732 None:intergenic 40.0%
! TTCTGTTTAGCTCCAGAATG+AGG - chr1.3:25878954-25878973 None:intergenic 40.0%
!!! GCCTTTTTGGAATTGAACGA+TGG + chr1.3:25878822-25878841 MS.gene65498:CDS 40.0%
AAGTCTGCAAACGCGATTTC+AGG - chr1.3:25879611-25879630 None:intergenic 45.0%
AATGGTACATTGACCTGAGG+CGG + chr1.3:25880116-25880135 MS.gene65498:CDS 45.0%
ACACGATATCTGCTGTTGCT+CGG + chr1.3:25879105-25879124 MS.gene65498:CDS 45.0%
AGAAAGCTAGAATCGGAGGA+TGG + chr1.3:25878763-25878782 MS.gene65498:CDS 45.0%
AGCAACAGCAGATATCGTGT+TGG - chr1.3:25879105-25879124 None:intergenic 45.0%
AGGAATATCGGGCTGAAGTT+TGG - chr1.3:25879402-25879421 None:intergenic 45.0%
CAATGATGACCTAGAGACAG+TGG + chr1.3:25879973-25879992 MS.gene65498:CDS 45.0%
CAGAGAATTCTCACACTTCC+AGG + chr1.3:25879558-25879577 MS.gene65498:CDS 45.0%
CCAAAATTCAGCCAAATGCC+TGG - chr1.3:25879579-25879598 None:intergenic 45.0%
CGGTCAGAGAATTCAGCTTT+GGG - chr1.3:25878687-25878706 None:intergenic 45.0%
CTGGAAGAATCTGCGAAGAA+TGG + chr1.3:25879812-25879831 MS.gene65498:CDS 45.0%
CTGTCTCTAGGTCATCATTG+AGG - chr1.3:25879973-25879992 None:intergenic 45.0%
GAAAGCTAGAATCGGAGGAT+GGG + chr1.3:25878764-25878783 MS.gene65498:CDS 45.0%
GATGTTCTTGTGCCAAAGGT+TGG + chr1.3:25880004-25880023 MS.gene65498:CDS 45.0%
GCAGGCAAGAAAGCTAGAAT+CGG + chr1.3:25878756-25878775 MS.gene65498:CDS 45.0%
GCTGAAGAGGTTCTTCATGT+TGG + chr1.3:25878993-25879012 MS.gene65498:CDS 45.0%
TACAGAAGCCGTGGAAATAC+TGG + chr1.3:25879793-25879812 MS.gene65498:CDS 45.0%
TATTCCTCGAAAGGACGGAA+AGG + chr1.3:25879415-25879434 MS.gene65498:CDS 45.0%
TCATTGAGGCGCATGTAGAA+TGG - chr1.3:25879959-25879978 None:intergenic 45.0%
TCCATAATCTGCCGTGAAGA+CGG + chr1.3:25878720-25878739 MS.gene65498:CDS 45.0%
TCCGTCCTTTCGAGGAATAT+CGG - chr1.3:25879414-25879433 None:intergenic 45.0%
TGAAATCGCGTTTGCAGACT+TGG + chr1.3:25879610-25879629 MS.gene65498:CDS 45.0%
TTCTCACACTTCCAGGCATT+TGG + chr1.3:25879565-25879584 MS.gene65498:CDS 45.0%
! AAACATGCTGGATTCGGTCT+TGG + chr1.3:25880151-25880170 MS.gene65498:CDS 45.0%
! CCAGGCATTTGGCTGAATTT+TGG + chr1.3:25879576-25879595 MS.gene65498:CDS 45.0%
! GAATGAGATTAGTGCTGCTG+AGG + chr1.3:25879370-25879389 MS.gene65498:CDS 45.0%
! GTCACGACTATTGATTGCGA+GGG + chr1.3:25879203-25879222 MS.gene65498:CDS 45.0%
! TCAAGAGGGAGTCTCCTTTT+AGG - chr1.3:25879047-25879066 None:intergenic 45.0%
! TGTCACGACTATTGATTGCG+AGG + chr1.3:25879202-25879221 MS.gene65498:CDS 45.0%
! TTATGGATTGAATCGGGACG+CGG - chr1.3:25878707-25878726 None:intergenic 45.0%
!! AAAGGCGAAAGCACCCTTAT+CGG - chr1.3:25878808-25878827 None:intergenic 45.0%
!! AGCAGATATCGTGTTGGTTC+TGG - chr1.3:25879099-25879118 None:intergenic 45.0%
!! GATTCGGTCTTGGCTTTGAA+AGG + chr1.3:25880161-25880180 MS.gene65498:CDS 45.0%
!!! TAAGGGTGCTTTCGCCTTTT+TGG + chr1.3:25878809-25878828 MS.gene65498:CDS 45.0%
AACTTGAAGGTTACCGGCAC+AGG - chr1.3:25878850-25878869 None:intergenic 50.0%
AATCGCGTTTGCAGACTTGG+AGG + chr1.3:25879613-25879632 MS.gene65498:CDS 50.0%
AATTGGTGGAAGCCAAAGGG+AGG + chr1.3:25880033-25880052 MS.gene65498:CDS 50.0%
AGAGGGTTACCGTGGATAAC+TGG - chr1.3:25878650-25878669 None:intergenic 50.0%
ATAATCTGCCGTGAAGACGG+CGG + chr1.3:25878723-25878742 MS.gene65498:CDS 50.0%
ATCGGAGGATGGGTGAAAAC+TGG + chr1.3:25878774-25878793 MS.gene65498:CDS 50.0%
ATTCCCTTCCCACGATATCC+TGG + chr1.3:25880226-25880245 MS.gene65498:CDS 50.0%
CATCATAACGCTCCTCCCTT+TGG - chr1.3:25880048-25880067 None:intergenic 50.0%
CCCGATATTCCTCGAAAGGA+CGG + chr1.3:25879410-25879429 MS.gene65498:CDS 50.0%
CCGTCCTTTCGAGGAATATC+GGG - chr1.3:25879413-25879432 None:intergenic 50.0%
GCGGTCAGAGAATTCAGCTT+TGG - chr1.3:25878688-25878707 None:intergenic 50.0%
GGATGATATGAAGTGCGCTG+AGG + chr1.3:25879634-25879653 MS.gene65498:CDS 50.0%
GGCAAGAAAGCTAGAATCGG+AGG + chr1.3:25878759-25878778 MS.gene65498:CDS 50.0%
GGTTACCGTGGATAACTGGT+CGG - chr1.3:25878646-25878665 None:intergenic 50.0%
TACATTGACCTGAGGCGGTA+TGG + chr1.3:25880121-25880140 MS.gene65498:CDS 50.0%
TCAGCCCGATATTCCTCGAA+AGG + chr1.3:25879406-25879425 MS.gene65498:CDS 50.0%
TGTAAGTCGAATGAAGGGGG+TGG - chr1.3:25879771-25879790 None:intergenic 50.0%
TTGAACGATGGAACCTGTGC+CGG + chr1.3:25878834-25878853 MS.gene65498:CDS 50.0%
! ACTATTGATTGCGAGGGTGC+TGG + chr1.3:25879209-25879228 MS.gene65498:CDS 50.0%
! GAAGGAGATTAGTGCTGCTG+TGG + chr1.3:25879294-25879313 MS.gene65498:CDS 50.0%
! GATTCTTTTTGCTACCGGCC+TGG + chr1.3:25880186-25880205 MS.gene65498:CDS 50.0%
! GGGAGTCTCCTTTTAGGCAA+AGG - chr1.3:25879041-25879060 None:intergenic 50.0%
!! TTCAGCTTTGGGAACCTCGT+CGG - chr1.3:25878676-25878695 None:intergenic 50.0%
AACCTGATCCGCCGTCTTCA+CGG - chr1.3:25878734-25878753 None:intergenic 55.0%
AAGTCGAATGAAGGGGGTGG+AGG - chr1.3:25879768-25879787 None:intergenic 55.0%
ACCGTCCGACCAGTTATCCA+CGG + chr1.3:25878638-25878657 MS.gene65498:CDS 55.0%
ACCGTGGATAACTGGTCGGA+CGG - chr1.3:25878642-25878661 None:intergenic 55.0%
CCATAGCAGCCACTGTCTCT+AGG - chr1.3:25879985-25880004 None:intergenic 55.0%
CCTAGAGACAGTGGCTGCTA+TGG + chr1.3:25879982-25880001 MS.gene65498:CDS 55.0%
CTGCCAGGATATCGTGGGAA+GGG - chr1.3:25880232-25880251 None:intergenic 55.0%
CTGCTCTGCCAGGATATCGT+GGG - chr1.3:25880237-25880256 None:intergenic 55.0%
GCCGTCTTCACGGCAGATTA+TGG - chr1.3:25878724-25878743 None:intergenic 55.0%
TCACTGTTCCATACCGCCTC+AGG - chr1.3:25880132-25880151 None:intergenic 55.0%
TCCACCTTTCCGTCCTTTCG+AGG - chr1.3:25879422-25879441 None:intergenic 55.0%
TCCTCGAAAGGACGGAAAGG+TGG + chr1.3:25879418-25879437 MS.gene65498:CDS 55.0%
TCTGCCAGGATATCGTGGGA+AGG - chr1.3:25880233-25880252 None:intergenic 55.0%
TCTGCTCTGCCAGGATATCG+TGG - chr1.3:25880238-25880257 None:intergenic 55.0%
!! ATTGCGAGGGTGCTGGTGAA+AGG + chr1.3:25879216-25879235 MS.gene65498:CDS 55.0%
GACGGCGGATCAGGTTTAGC+AGG + chr1.3:25878738-25878757 MS.gene65498:CDS 60.0%
GATCGAGCTTCGCGCTGAAG+AGG + chr1.3:25878980-25878999 MS.gene65498:CDS 60.0%
GTGGATAACTGGTCGGACGG+TGG - chr1.3:25878639-25878658 None:intergenic 60.0%
!! AGCTTTGGGAACCTCGTCGG+AGG - chr1.3:25878673-25878692 None:intergenic 60.0%
GGGAACCTCGTCGGAGGAGA+GGG - chr1.3:25878667-25878686 None:intergenic 65.0%
GGTAACCCTCTCCTCCGACG+AGG + chr1.3:25878659-25878678 MS.gene65498:CDS 65.0%
GTCGGAGGAGAGGGTTACCG+TGG - chr1.3:25878658-25878677 None:intergenic 65.0%
TGCCGTGAAGACGGCGGATC+AGG + chr1.3:25878729-25878748 MS.gene65498:CDS 65.0%
TGGGAACCTCGTCGGAGGAG+AGG - chr1.3:25878668-25878687 None:intergenic 65.0%
! GGTCGGACGGTGGTGATGAG+AGG - chr1.3:25878629-25878648 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 25878618 25880264 25878618 ID=MS.gene65498
chr1.3 mRNA 25878618 25880264 25878618 ID=MS.gene65498.t1;Parent=MS.gene65498
chr1.3 exon 25878618 25880264 25878618 ID=MS.gene65498.t1.exon1;Parent=MS.gene65498.t1
chr1.3 CDS 25878618 25880264 25878618 ID=cds.MS.gene65498.t1;Parent=MS.gene65498.t1
Gene Sequence

>MS.gene65498

ATGTCAGACCTCTCATCACCACCGTCCGACCAGTTATCCACGGTAACCCTCTCCTCCGACGAGGTTCCCAAAGCTGAATTCTCTGACCGCGTCCCGATTCAATCCATAATCTGCCGTGAAGACGGCGGATCAGGTTTAGCAGGCAAGAAAGCTAGAATCGGAGGATGGGTGAAAACTGGAAGAAAAGCCGATAAGGGTGCTTTCGCCTTTTTGGAATTGAACGATGGAACCTGTGCCGGTAACCTTCAAGTTATCGTTGAAGCTTCGCTTGCTGATCTTAGACAACTTGTTCTTACTGGAACTTGTGTAGTTGTTGATGGACATCTCAAACTTCCTCATTCTGGAGCTAAACAGAAAAAGAAGATCGAGCTTCGCGCTGAAGAGGTTCTTCATGTTGGTCCTGTTGATCCTGCTAAATATCCTTTGCCTAAAAGGAGACTCCCTCTTGAAGTTTTGCGTGATTTCGTTCATCTTCGTTCCAGAACCAACACGATATCTGCTGTTGCTCGGATCAGTAATGCTCTTGCTTATGCTACACATACATTTTTCAACAAGCATGGTTTTCTTCATGTGCACACGCCAATTGTCACGACTATTGATTGCGAGGGTGCTGGTGAAAGGTTTCAAGTGACAACTTTGTTCGATGTTGAAGCTGTTGTTAAGGAGAAGGGGATAAGAAGGAGATTAGTGCTGCTGTGGATGAAATTAAAAAAGCTGGTTGTTTCAAAATTGAAATCCGCAAAAGCAGATAAGAATGAGATTAGTGCTGCTGAGGAAAGATCCAAACTTCAGCCCGATATTCCTCGAAAGGACGGAAAGGTGGATTATACTCAAGATTTCTTTGCGCGTCAAGCATTTTTGACTGTTTCTGGTCAGCTTCAAGCTGAGTCTTATGCGTGTGCTCTTAGTAGTGTGTATACATTTGGCCCGACATTTCGAGCAGAGAATTCTCACACTTCCAGGCATTTGGCTGAATTTTGGATGGTAGAACCTGAAATCGCGTTTGCAGACTTGGAGGATGATATGAAGTGCGCTGAGGCATATGTCAAATTTTTGTGTCAGTGGTTACTTGACAACTGTCTTGAGGATATGCAATTTATGGCTGATAAGGTTGATAAAGGTTGCATCGATCGTTTGAAAATGGTTGCCTCCACCCCCTTCATTCGACTTACATATACAGAAGCCGTGGAAATACTGGAAGAATCTGCGAAGAATGGTCAGAAATTTGAGAAGGAAGTAAAATGGGGAATCGATTTAGCTTCTGAGCATGAAAGATACCTAACAGAGACGAAGTATAAGAAGCCTGTCATAGTCTATAATTATCCAAAAGGTATCAAACCATTCTACATGCGCCTCAATGATGACCTAGAGACAGTGGCTGCTATGGATGTTCTTGTGCCAAAGGTTGGAGAGTTAATTGGTGGAAGCCAAAGGGAGGAGCGTTATGATGTCATTCAACAAGGACTAAAGGAGAAGAATCTGCCTGTTAAGCCATATCAATGGTACATTGACCTGAGGCGGTATGGAACAGTGAAACATGCTGGATTCGGTCTTGGCTTTGAAAGGATGATTCTTTTTGCTACCGGCCTGGAAAATATTCGAGATGTGATTCCCTTCCCACGATATCCTGGCAGAGCAGATTTATGA
Protein sequence

>MS.gene65498.t1

MSDLSSPPSDQLSTVTLSSDEVPKAEFSDRVPIQSIICREDGGSGLAGKKARIGGWVKTGRKADKGAFAFLELNDGTCAGNLQVIVEASLADLRQLVLTGTCVVVDGHLKLPHSGAKQKKKIELRAEEVLHVGPVDPAKYPLPKRRLPLEVLRDFVHLRSRTNTISAVARISNALAYATHTFFNKHGFLHVHTPIVTTIDCEGAGERFQVTTLFDVEAVVKEKGIRRRLVLLWMKLKKLVVSKLKSAKADKNEISAAEERSKLQPDIPRKDGKVDYTQDFFARQAFLTVSGQLQAESYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMKCAEAYVKFLCQWLLDNCLEDMQFMADKVDKGCIDRLKMVASTPFIRLTYTEAVEILEESAKNGQKFEKEVKWGIDLASEHERYLTETKYKKPVIVYNYPKGIKPFYMRLNDDLETVAAMDVLVPKVGELIGGSQREERYDVIQQGLKEKNLPVKPYQWYIDLRRYGTVKHAGFGLGFERMILFATGLENIRDVIPFPRYPGRADL