AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81617

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81617.t1 MTR_5g085480 68.304 448 108 5 37 454 7 450 0.0 588
MS.gene81617.t1 MTR_2g033050 64.390 410 110 6 79 456 3 408 3.08e-173 492
MS.gene81617.t1 MTR_5g085810 53.092 469 154 10 38 448 43 503 2.53e-150 445
MS.gene81617.t1 MTR_5g085830 55.909 440 146 9 38 443 47 472 8.92e-148 438
MS.gene81617.t1 MTR_5g085520 84.694 196 28 1 85 280 2 195 4.22e-117 342
MS.gene81617.t1 MTR_5g085480 62.329 292 78 4 193 454 1 290 1.16e-113 345
MS.gene81617.t1 MTR_5g085490 63.701 281 92 6 13 289 17 291 1.61e-111 330
MS.gene81617.t1 MTR_5g085500 69.143 175 53 1 281 454 5 179 7.60e-77 248
MS.gene81617.t1 MTR_5g085440 62.893 159 54 3 1 157 1 156 5.40e-53 182
MS.gene81617.t1 MTR_5g085530 83.721 43 6 1 286 327 31 73 4.17e-16 73.6
MS.gene81617.t1 MTR_8g040930 28.824 170 96 4 274 429 23 181 1.70e-13 72.0
MS.gene81617.t1 MTR_8g041240 28.824 170 96 4 274 429 23 181 1.70e-13 72.0
MS.gene81617.t1 MTR_8g072510 27.647 170 95 5 274 429 74 229 5.11e-13 71.2
MS.gene81617.t1 MTR_8g467780 31.073 177 88 5 275 429 76 240 6.37e-13 70.9
MS.gene81617.t1 MTR_3g016210 27.586 174 105 5 275 432 75 243 4.71e-12 68.2
MS.gene81617.t1 MTR_8g066670 28.877 187 116 5 274 452 1139 1316 1.35e-11 67.4
MS.gene81617.t1 MTR_7g021090 28.659 164 101 4 274 429 73 228 1.99e-11 65.9
MS.gene81617.t1 MTR_2g096190 24.648 284 184 7 145 428 23 276 2.24e-11 66.2
MS.gene81617.t1 MTR_7g451420 28.426 197 116 6 274 455 73 259 2.39e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81617.t1 AT1G03670 27.913 369 197 10 86 429 73 397 1.27e-31 128
MS.gene81617.t1 AT4G03500 29.491 373 235 9 64 429 53 404 9.46e-31 125
MS.gene81617.t1 AT4G03500 29.491 373 235 9 64 429 53 404 9.46e-31 125
MS.gene81617.t1 AT4G03500 29.491 373 235 9 64 429 76 427 1.08e-30 125
MS.gene81617.t1 AT4G03440 28.134 359 208 9 86 406 126 472 2.38e-29 122
MS.gene81617.t1 AT4G03440 28.134 359 208 9 86 406 119 465 2.58e-29 122
MS.gene81617.t1 AT4G03440 28.134 359 208 9 86 406 119 465 2.58e-29 122
MS.gene81617.t1 AT4G03460 43.662 142 77 2 289 429 241 380 1.32e-26 113
MS.gene81617.t1 AT4G03460 43.662 142 77 2 289 429 273 412 1.47e-26 113
MS.gene81617.t1 AT4G03480 25.858 437 237 12 59 430 126 540 2.83e-24 106
MS.gene81617.t1 AT4G03480 25.576 434 239 12 59 430 126 537 4.82e-24 105
MS.gene81617.t1 AT4G03450 27.989 368 211 11 84 429 70 405 5.36e-24 105
MS.gene81617.t1 AT4G03450 27.989 368 211 11 84 429 70 405 7.60e-24 105
MS.gene81617.t1 AT4G05040 27.515 338 193 8 80 394 110 418 3.56e-23 102
MS.gene81617.t1 AT4G05040 27.515 338 193 8 80 394 110 418 3.56e-23 102
MS.gene81617.t1 AT4G05040 27.515 338 193 8 80 394 110 418 3.56e-23 102
MS.gene81617.t1 AT4G05040 27.515 338 193 8 80 394 110 418 3.56e-23 102
MS.gene81617.t1 AT4G05040 27.515 338 193 8 80 394 110 418 3.56e-23 102
MS.gene81617.t1 AT4G05040 27.515 338 193 8 80 394 110 418 3.56e-23 102
MS.gene81617.t1 AT4G05040 27.515 338 193 8 80 394 110 418 3.56e-23 102
MS.gene81617.t1 AT4G03470 29.014 355 213 11 86 429 120 446 4.27e-23 102
MS.gene81617.t1 AT4G03490 27.481 393 211 14 77 428 28 387 1.43e-22 101
MS.gene81617.t1 AT4G03490 27.481 393 211 14 77 428 18 377 1.97e-22 100
MS.gene81617.t1 AT4G14400 26.146 371 227 10 80 429 30 374 2.38e-22 100
MS.gene81617.t1 AT4G14400 26.146 371 227 10 80 429 30 374 2.38e-22 100
MS.gene81617.t1 AT4G03490 27.296 392 213 13 77 428 28 387 2.48e-22 100
MS.gene81617.t1 AT4G14400 26.075 372 228 10 80 430 96 441 2.55e-22 100
MS.gene81617.t1 AT4G14390 26.829 369 223 11 80 429 108 448 2.29e-21 97.1
MS.gene81617.t1 AT4G14390 26.558 369 224 10 80 429 108 448 2.55e-21 97.4
MS.gene81617.t1 AT4G14390 26.558 369 224 10 80 429 127 467 3.34e-21 97.1
MS.gene81617.t1 AT4G14390 26.757 370 223 11 80 429 127 468 4.72e-20 93.6
MS.gene81617.t1 AT4G03490 27.397 365 193 13 77 400 8 341 8.58e-20 92.8
MS.gene81617.t1 AT4G03490 27.198 364 195 12 77 400 18 351 8.89e-20 92.8
MS.gene81617.t1 AT5G60070 25.000 316 176 9 116 428 29 286 5.47e-12 68.2
MS.gene81617.t1 AT1G07710 29.798 198 129 5 232 428 88 276 4.30e-11 65.5
MS.gene81617.t1 AT1G07710 29.798 198 129 5 232 428 88 276 4.30e-11 65.5

Find 83 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGCAAACTCACAAATTTAT+AGG 0.196436 5.1:+67495492 MS.gene81617:CDS
TTACCTTGCGGTTGAAAATA+AGG 0.222472 5.1:+67493441 MS.gene81617:CDS
TCATTATCAGATTGTTGTTA+TGG 0.268452 5.1:+67493376 MS.gene81617:CDS
TCCGGCTTAGCATCATTCTT+AGG 0.271780 5.1:-67493499 None:intergenic
TTCAATATCTATTAAATTAG+AGG 0.278846 5.1:-67492918 None:intergenic
CCAGAAGATGCTTTATGAAT+TGG 0.281637 5.1:-67495096 None:intergenic
TTAAATTTGAACAAAAGTTT+TGG 0.284191 5.1:-67493022 None:intergenic
CCAATTCATAAAGCATCTTC+TGG 0.305031 5.1:+67495096 MS.gene81617:CDS
CTACGTTGAGGAGTTAAATA+TGG 0.357336 5.1:+67493204 MS.gene81617:CDS
CCTTGTTCACAAAACATAAC+AGG 0.357712 5.1:-67493231 None:intergenic
CTTTCTGATTTATCATCTTA+CGG 0.366548 5.1:-67495272 None:intergenic
TTATAATATGGCTCCTAGTC+TGG 0.372646 5.1:+67492691 None:intergenic
ATTACGCATGCAGCATCTAT+AGG 0.378008 5.1:+67494997 MS.gene81617:intron
TGTCGTGACATTAAGAGAAT+TGG 0.382899 5.1:+67493295 MS.gene81617:CDS
ACAAGACTGTAGATCGGTTC+GGG 0.384695 5.1:+67493350 MS.gene81617:CDS
TCAAGCATCTCTGTGGGATC+TGG 0.393203 5.1:-67495159 None:intergenic
ATACAAGAAAATCAAGTTCA+TGG 0.397897 5.1:+67495246 MS.gene81617:CDS
AGATGATAAATCAGAAAGAT+AGG 0.425002 5.1:+67495277 MS.gene81617:CDS
GGATGCAGTCAAACTAATCT+TGG 0.425296 5.1:+67493468 MS.gene81617:CDS
TACAAGACTGTAGATCGGTT+CGG 0.429343 5.1:+67493349 MS.gene81617:CDS
ACAATTCAAAGTGACATTGA+TGG 0.442706 5.1:+67495066 MS.gene81617:CDS
TGGAGGTTCAACGAATTTGT+TGG 0.446313 5.1:+67492892 MS.gene81617:CDS
TGCTTCATGATAGCTTCAAC+AGG 0.446814 5.1:-67493532 None:intergenic
GTAATAATTCAACACCTTCA+AGG 0.447605 5.1:-67495023 None:intergenic
CCGGCTTAGCATCATTCTTA+GGG 0.447690 5.1:-67493498 None:intergenic
CTTCACATTGCATCAATCAA+TGG 0.448810 5.1:+67495201 MS.gene81617:CDS
GGAGGTTCAACGAATTTGTT+GGG 0.452189 5.1:+67492893 MS.gene81617:CDS
GAAGGTGTTGAATTATTACT+TGG 0.460344 5.1:+67495027 MS.gene81617:CDS
GAAGCATATCACTTGGTGTA+TGG 0.463790 5.1:+67492872 MS.gene81617:CDS
ACCTCTTGCAGCAACATGTA+AGG 0.469580 5.1:-67493062 None:intergenic
ATCTTGCAGCGAAATGCAAA+GGG 0.487254 5.1:-67495311 None:intergenic
GCATCTATAGGTTACCTTGA+AGG 0.499660 5.1:+67495009 MS.gene81617:intron
AACTTGATTGTCAAATGCGC+AGG 0.501739 5.1:-67492837 None:intergenic
CCCTAAGAATGATGCTAAGC+CGG 0.505153 5.1:+67493498 MS.gene81617:CDS
TATAAATTTGTGAGTTTGCG+AGG 0.522447 5.1:-67495490 None:intergenic
CTACACATTGCAGCTAGGTA+TGG 0.528030 5.1:+67492962 MS.gene81617:CDS
TGATAAATCATCTTTGAGAC+AGG 0.538034 5.1:+67495452 MS.gene81617:CDS
AGACTCCATCTTGCACTAGT+TGG 0.542484 5.1:-67492733 None:intergenic
GGTTAACCAGGATAACAAAA+CGG 0.549876 5.1:+67495398 MS.gene81617:CDS
GTGATCTCAGCGACTTCTGA+GGG 0.550022 5.1:-67492812 None:intergenic
TTGCGGTTGAAAATAAGGAC+AGG 0.552247 5.1:+67493446 MS.gene81617:CDS
GATCTTGCAGCGAAATGCAA+AGG 0.553477 5.1:-67495312 None:intergenic
CGTGACATTAAGAGAATTGG+CGG 0.557041 5.1:+67493298 MS.gene81617:CDS
GTGAACAAGGAGAATGCTCA+AGG 0.562389 5.1:+67493244 MS.gene81617:CDS
GAAATCTGTAATTTACCTTG+CGG 0.563641 5.1:+67493429 MS.gene81617:CDS
GCTTAGTGAAGCATATCACT+TGG 0.563927 5.1:+67492865 MS.gene81617:CDS
TCAACAGGTGATAATCCCTC+CGG 0.566490 5.1:-67493517 None:intergenic
ACTAAGTAGAACACAGTCAT+GGG 0.567710 5.1:-67495342 None:intergenic
ATAAATCAGAAAGATAGGAA+TGG 0.570805 5.1:+67495282 MS.gene81617:CDS
GCATCTTGATATGGTTAACC+AGG 0.573041 5.1:+67495386 MS.gene81617:CDS
ATAAATTTGTGAGTTTGCGA+GGG 0.575667 5.1:-67495489 None:intergenic
AACTAAGTAGAACACAGTCA+TGG 0.578346 5.1:-67495343 None:intergenic
GCCTTACATGTTGCTGCAAG+AGG 0.579006 5.1:+67493061 MS.gene81617:CDS
AGTGATCTCAGCGACTTCTG+AGG 0.588926 5.1:-67492813 None:intergenic
GTGACATTAAGAGAATTGGC+GGG 0.591161 5.1:+67493299 MS.gene81617:CDS
TGAACGGCGTGATATAAAAG+AGG 0.592876 5.1:+67493132 MS.gene81617:CDS
TTACATGTTGCTGCAAGAGG+TGG 0.595346 5.1:+67493064 MS.gene81617:CDS
CAGTGCTACACATTGCAGCT+AGG 0.599665 5.1:+67492957 MS.gene81617:CDS
TTAGACCAACTAGTGCAAGA+TGG 0.608063 5.1:+67492728 MS.gene81617:CDS
AACTTTACAAGACTGTAGAT+CGG 0.608279 5.1:+67493344 MS.gene81617:CDS
AGCATCTTCTGGTGGCCATG+TGG 0.614596 5.1:+67495107 MS.gene81617:CDS
AAGAATGATGCTAAGCCGGA+GGG 0.615867 5.1:+67493502 MS.gene81617:CDS
ATGAGTGTCAAGCATCTCTG+TGG 0.624243 5.1:-67495166 None:intergenic
TGAGTGTCAAGCATCTCTGT+GGG 0.627689 5.1:-67495165 None:intergenic
GCATATCACTTGGTGTATGG+AGG 0.627873 5.1:+67492875 MS.gene81617:CDS
GGTTAGAATACACTAACCGT+TGG 0.635092 5.1:+67493158 MS.gene81617:CDS
ACTTGATTGTCAAATGCGCA+GGG 0.647605 5.1:-67492836 None:intergenic
TAAATTTGTGAGTTTGCGAG+GGG 0.649173 5.1:-67495488 None:intergenic
TAAGAATGATGCTAAGCCGG+AGG 0.649636 5.1:+67493501 MS.gene81617:CDS
AAAATTATACTAATACTGAA+CGG 0.654942 5.1:+67493116 MS.gene81617:CDS
CGTTGAGGAGTTAAATATGG+AGG 0.656599 5.1:+67493207 MS.gene81617:CDS
TAATATGGCTCCTAGTCTGG+AGG 0.658481 5.1:+67492694 None:intergenic
TGACATTAAGAGAATTGGCG+GGG 0.659341 5.1:+67493300 MS.gene81617:CDS
GGCGTGATATAAAAGAGGCA+TGG 0.660331 5.1:+67493137 MS.gene81617:CDS
TGCGGTTGAAAATAAGGACA+GGG 0.661212 5.1:+67493447 MS.gene81617:CDS
GCTATCATGAAGCATAATCA+CGG 0.661947 5.1:+67493541 MS.gene81617:CDS
CATCTTCTACATCACTCCAA+CGG 0.664221 5.1:-67493174 None:intergenic
GCAAGATGGAGTCTTACCAA+AGG 0.664709 5.1:+67492742 MS.gene81617:CDS
ATTCATAAAGCATCTTCTGG+TGG 0.683443 5.1:+67495099 MS.gene81617:CDS
ACTTCTTCACTACTTCCACA+TGG 0.684018 5.1:-67495122 None:intergenic
AGATGTAGAAGACTACGTTG+AGG 0.688400 5.1:+67493192 MS.gene81617:CDS
GTGAATCTTGTAATTGAACG+TGG 0.747086 5.1:+67492998 MS.gene81617:CDS
TCTTGCAGCGAAATGCAAAG+GGG 0.761035 5.1:-67495310 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTCTTTAATTTTTTTTTAT+CGG - chr5.1:67494164-67494183 None:intergenic 10.0%
!! AAAATTATACTAATACTGAA+CGG + chr5.1:67493116-67493135 MS.gene81617:CDS 15.0%
!! AAATCATAAAAGAAATTGTA+AGG - chr5.1:67493682-67493701 None:intergenic 15.0%
!! CAGATAAATTAAAAGAATTA+AGG + chr5.1:67494227-67494246 MS.gene81617:intron 15.0%
!! TTCAATATCTATTAAATTAG+AGG - chr5.1:67492921-67492940 None:intergenic 15.0%
!!! ATTTTCTAATAGCTTTTTAA+CGG - chr5.1:67493101-67493120 None:intergenic 15.0%
!!! ATTTTTGACCTTTTTTTTAT+AGG + chr5.1:67494192-67494211 MS.gene81617:intron 15.0%
!!! CTTAATTCTTTTAATTTATC+TGG - chr5.1:67494229-67494248 None:intergenic 15.0%
!!! TACATTTTATGACTAATTAA+AGG + chr5.1:67494073-67494092 MS.gene81617:intron 15.0%
!!! TTAAATTTGAACAAAAGTTT+TGG - chr5.1:67493025-67493044 None:intergenic 15.0%
!!! TTACAATTTCTTTTATGATT+TGG + chr5.1:67493681-67493700 MS.gene81617:intron 15.0%
!! AAAAAAGGTCAAAAATATCA+TGG - chr5.1:67494188-67494207 None:intergenic 20.0%
!! AAAAAGGTCAAAAATATCAT+GGG - chr5.1:67494187-67494206 None:intergenic 20.0%
!! ATTGTGTTCCTATAAAAAAA+AGG - chr5.1:67494203-67494222 None:intergenic 20.0%
!! GTTTGAATGAAAAATGTTAA+TGG + chr5.1:67494046-67494065 MS.gene81617:intron 20.0%
!! TTGAAATGACAAATTTCAAA+CGG - chr5.1:67494574-67494593 None:intergenic 20.0%
!!! ACTGAGAGTAAATATTTTAT+AGG + chr5.1:67493607-67493626 MS.gene81617:intron 20.0%
! AAAAGGTCAAAAATATCATG+GGG - chr5.1:67494186-67494205 None:intergenic 25.0%
! ACGTTTATTGTTAAATTGGT+AGG - chr5.1:67494003-67494022 None:intergenic 25.0%
! AGATGATAAATCAGAAAGAT+AGG + chr5.1:67495277-67495296 MS.gene81617:CDS 25.0%
! ATAAATCAGAAAGATAGGAA+TGG + chr5.1:67495282-67495301 MS.gene81617:CDS 25.0%
! ATACAAGAAAATCAAGTTCA+TGG + chr5.1:67495246-67495265 MS.gene81617:CDS 25.0%
! ATCCATTTAAGGAACAAATA+TGG + chr5.1:67494960-67494979 MS.gene81617:intron 25.0%
! CAATCATCAATTGAATTCTA+AGG - chr5.1:67494540-67494559 None:intergenic 25.0%
! CTTTCTGATTTATCATCTTA+CGG - chr5.1:67495275-67495294 None:intergenic 25.0%
! GGAAAACATAAAAAAAATGC+AGG - chr5.1:67493852-67493871 None:intergenic 25.0%
! GTAATCATATTTGTAAATGC+AGG - chr5.1:67493826-67493845 None:intergenic 25.0%
! GTGAACGTTTATTGTTAAAT+TGG - chr5.1:67494007-67494026 None:intergenic 25.0%
! TAAGTACTAGAAAAATCCTT+TGG - chr5.1:67492761-67492780 None:intergenic 25.0%
! TATGATACATAAACAACCAT+AGG - chr5.1:67494875-67494894 None:intergenic 25.0%
! TGACAAAATGGGTAATATTT+AGG + chr5.1:67494707-67494726 MS.gene81617:intron 25.0%
! TGAGACTAATAAAAAAAGAG+CGG - chr5.1:67494408-67494427 None:intergenic 25.0%
! TTCCATATTTGTTCCTTAAA+TGG - chr5.1:67494965-67494984 None:intergenic 25.0%
!! TCATTATCAGATTGTTGTTA+TGG + chr5.1:67493376-67493395 MS.gene81617:CDS 25.0%
!!! ATTTATCGCTCTCTAAAATA+TGG - chr5.1:67493975-67493994 None:intergenic 25.0%
!!! GGATTTTTCTAGTACTTATA+AGG + chr5.1:67492763-67492782 MS.gene81617:CDS 25.0%
!!! TTTCTTTTGTTTGTGTTTGT+TGG + chr5.1:67493727-67493746 MS.gene81617:intron 25.0%
AACTTTACAAGACTGTAGAT+CGG + chr5.1:67493344-67493363 MS.gene81617:CDS 30.0%
ACAATTCAAAGTGACATTGA+TGG + chr5.1:67495066-67495085 MS.gene81617:CDS 30.0%
AGCGAAATACAAATTTACGA+TGG - chr5.1:67494647-67494666 None:intergenic 30.0%
ATAAATTTGTGAGTTTGCGA+GGG - chr5.1:67495492-67495511 None:intergenic 30.0%
GAAATCTGTAATTTACCTTG+CGG + chr5.1:67493429-67493448 MS.gene81617:CDS 30.0%
GTAATAATTCAACACCTTCA+AGG - chr5.1:67495026-67495045 None:intergenic 30.0%
TACTACAAAACAAACCAACT+TGG + chr5.1:67494937-67494956 MS.gene81617:intron 30.0%
TATAAATTTGTGAGTTTGCG+AGG - chr5.1:67495493-67495512 None:intergenic 30.0%
TCGCAAACTCACAAATTTAT+AGG + chr5.1:67495492-67495511 MS.gene81617:CDS 30.0%
! TTTTGTACACCTCATACATT+AGG + chr5.1:67494765-67494784 MS.gene81617:intron 30.0%
!! GAAGGTGTTGAATTATTACT+TGG + chr5.1:67495027-67495046 MS.gene81617:CDS 30.0%
!! TGATAAATCATCTTTGAGAC+AGG + chr5.1:67495452-67495471 MS.gene81617:CDS 30.0%
!!! AGTTGGTTTGTTTTGTAGTA+TGG - chr5.1:67494937-67494956 None:intergenic 30.0%
!!! TTTTTTTTTTCCTCCAGACT+AGG - chr5.1:67492707-67492726 None:intergenic 30.0%
AACCAACTTGGATCCATTTA+AGG + chr5.1:67494949-67494968 MS.gene81617:intron 35.0%
AACTAAGTAGAACACAGTCA+TGG - chr5.1:67495346-67495365 None:intergenic 35.0%
ACAAGTACCCTAATTGTATG+GGG - chr5.1:67494688-67494707 None:intergenic 35.0%
ACTAAGTAGAACACAGTCAT+GGG - chr5.1:67495345-67495364 None:intergenic 35.0%
ATACATTAGGAAGAGAGAAG+AGG + chr5.1:67494778-67494797 MS.gene81617:intron 35.0%
CACAAGTACCCTAATTGTAT+GGG - chr5.1:67494689-67494708 None:intergenic 35.0%
CCAGAAGATGCTTTATGAAT+TGG - chr5.1:67495099-67495118 None:intergenic 35.0%
CCTTGTTCACAAAACATAAC+AGG - chr5.1:67493234-67493253 None:intergenic 35.0%
CTACGTTGAGGAGTTAAATA+TGG + chr5.1:67493204-67493223 MS.gene81617:CDS 35.0%
CTTCACATTGCATCAATCAA+TGG + chr5.1:67495201-67495220 MS.gene81617:CDS 35.0%
GCTATCATGAAGCATAATCA+CGG + chr5.1:67493541-67493560 MS.gene81617:CDS 35.0%
GGTTAACCAGGATAACAAAA+CGG + chr5.1:67495398-67495417 MS.gene81617:CDS 35.0%
GTGAATCTTGTAATTGAACG+TGG + chr5.1:67492998-67493017 MS.gene81617:CDS 35.0%
TAAATTTGTGAGTTTGCGAG+GGG - chr5.1:67495491-67495510 None:intergenic 35.0%
TCACAAGTACCCTAATTGTA+TGG - chr5.1:67494690-67494709 None:intergenic 35.0%
TGAACTTCCCCATACAATTA+GGG + chr5.1:67494678-67494697 MS.gene81617:intron 35.0%
TGTCGTGACATTAAGAGAAT+TGG + chr5.1:67493295-67493314 MS.gene81617:CDS 35.0%
TTACCTTGCGGTTGAAAATA+AGG + chr5.1:67493441-67493460 MS.gene81617:CDS 35.0%
TTCCTTAAATGGATCCAAGT+TGG - chr5.1:67494954-67494973 None:intergenic 35.0%
TTCTCTCTTCCTAATGTATG+AGG - chr5.1:67494777-67494796 None:intergenic 35.0%
TTGAACTTCCCCATACAATT+AGG + chr5.1:67494677-67494696 MS.gene81617:intron 35.0%
! ATGAAAGAGAAAGTGTGCTT+GGG + chr5.1:67494803-67494822 MS.gene81617:intron 35.0%
! ATTCATAAAGCATCTTCTGG+TGG + chr5.1:67495099-67495118 MS.gene81617:CDS 35.0%
! CCAATTCATAAAGCATCTTC+TGG + chr5.1:67495096-67495115 MS.gene81617:CDS 35.0%
! CCTGTTATGTTTTGTGAACA+AGG + chr5.1:67493231-67493250 MS.gene81617:CDS 35.0%
! CGAAAAAGTGCATCTTGATA+TGG + chr5.1:67495377-67495396 MS.gene81617:CDS 35.0%
! GTAGCACTGTATTTTTCGTA+GGG - chr5.1:67492946-67492965 None:intergenic 35.0%
! TAGCACTGTATTTTTCGTAG+GGG - chr5.1:67492945-67492964 None:intergenic 35.0%
! TAGGGTACTTGTGACAAAAT+GGG + chr5.1:67494696-67494715 MS.gene81617:intron 35.0%
! TGAGGTGTACAAAAGTGTAT+AGG - chr5.1:67494759-67494778 None:intergenic 35.0%
! TGTAGCACTGTATTTTTCGT+AGG - chr5.1:67492947-67492966 None:intergenic 35.0%
! TTAGGGTACTTGTGACAAAA+TGG + chr5.1:67494695-67494714 MS.gene81617:intron 35.0%
!! AAAAAAAGAGCGGTGCTATA+TGG - chr5.1:67494398-67494417 None:intergenic 35.0%
!!! GATGCACTTTTTCGTTGTTT+TGG - chr5.1:67495371-67495390 None:intergenic 35.0%
AACTTGATTGTCAAATGCGC+AGG - chr5.1:67492840-67492859 None:intergenic 40.0%
ACTTCTTCACTACTTCCACA+TGG - chr5.1:67495125-67495144 None:intergenic 40.0%
ACTTGATTGTCAAATGCGCA+GGG - chr5.1:67492839-67492858 None:intergenic 40.0%
AGATGTAGAAGACTACGTTG+AGG + chr5.1:67493192-67493211 MS.gene81617:CDS 40.0%
ATCTTGCAGCGAAATGCAAA+GGG - chr5.1:67495314-67495333 None:intergenic 40.0%
ATTACGCATGCAGCATCTAT+AGG + chr5.1:67494997-67495016 MS.gene81617:intron 40.0%
CATCTTCTACATCACTCCAA+CGG - chr5.1:67493177-67493196 None:intergenic 40.0%
CGTGACATTAAGAGAATTGG+CGG + chr5.1:67493298-67493317 MS.gene81617:CDS 40.0%
CGTTGAGGAGTTAAATATGG+AGG + chr5.1:67493207-67493226 MS.gene81617:CDS 40.0%
GAAGCATAATCACGGTACAT+TGG + chr5.1:67493549-67493568 MS.gene81617:intron 40.0%
GCATCTATAGGTTACCTTGA+AGG + chr5.1:67495009-67495028 MS.gene81617:intron 40.0%
GCATCTTGATATGGTTAACC+AGG + chr5.1:67495386-67495405 MS.gene81617:CDS 40.0%
GCTTAGTGAAGCATATCACT+TGG + chr5.1:67492865-67492884 MS.gene81617:CDS 40.0%
GGATGCAGTCAAACTAATCT+TGG + chr5.1:67493468-67493487 MS.gene81617:CDS 40.0%
GGTTAGAATACACTAACCGT+TGG + chr5.1:67493158-67493177 MS.gene81617:CDS 40.0%
GTGACATTAAGAGAATTGGC+GGG + chr5.1:67493299-67493318 MS.gene81617:CDS 40.0%
TACAAGACTGTAGATCGGTT+CGG + chr5.1:67493349-67493368 MS.gene81617:CDS 40.0%
TGAACGGCGTGATATAAAAG+AGG + chr5.1:67493132-67493151 MS.gene81617:CDS 40.0%
TGACATTAAGAGAATTGGCG+GGG + chr5.1:67493300-67493319 MS.gene81617:CDS 40.0%
TGCGGTTGAAAATAAGGACA+GGG + chr5.1:67493447-67493466 MS.gene81617:CDS 40.0%
TGCTTCATGATAGCTTCAAC+AGG - chr5.1:67493535-67493554 None:intergenic 40.0%
TGGAGGTTCAACGAATTTGT+TGG + chr5.1:67492892-67492911 MS.gene81617:CDS 40.0%
TTAGACCAACTAGTGCAAGA+TGG + chr5.1:67492728-67492747 MS.gene81617:CDS 40.0%
TTGCGGTTGAAAATAAGGAC+AGG + chr5.1:67493446-67493465 MS.gene81617:CDS 40.0%
! AAGAGGTGTATTGTCACCTA+TGG + chr5.1:67494856-67494875 MS.gene81617:intron 40.0%
! CACGTATCAGATTTTTGCCT+GGG + chr5.1:67494829-67494848 MS.gene81617:intron 40.0%
! GATGAAAGAGAAAGTGTGCT+TGG + chr5.1:67494802-67494821 MS.gene81617:intron 40.0%
! GGAGGTTCAACGAATTTGTT+GGG + chr5.1:67492893-67492912 MS.gene81617:CDS 40.0%
! TGAAAGAGAAAGTGTGCTTG+GGG + chr5.1:67494804-67494823 MS.gene81617:intron 40.0%
!! GAAGCATATCACTTGGTGTA+TGG + chr5.1:67492872-67492891 MS.gene81617:CDS 40.0%
!!! TGTCCTTATTTTCAACCGCA+AGG - chr5.1:67493447-67493466 None:intergenic 40.0%
AAGAATGATGCTAAGCCGGA+GGG + chr5.1:67493502-67493521 MS.gene81617:CDS 45.0%
ACCTCTTGCAGCAACATGTA+AGG - chr5.1:67493065-67493084 None:intergenic 45.0%
AGACTCCATCTTGCACTAGT+TGG - chr5.1:67492736-67492755 None:intergenic 45.0%
ATGAGTGTCAAGCATCTCTG+TGG - chr5.1:67495169-67495188 None:intergenic 45.0%
ATGCTGCATGCGTAATGAAG+AGG - chr5.1:67494993-67495012 None:intergenic 45.0%
CCAGGCAAAAATCTGATACG+TGG - chr5.1:67494831-67494850 None:intergenic 45.0%
CCCTAAGAATGATGCTAAGC+CGG + chr5.1:67493498-67493517 MS.gene81617:CDS 45.0%
CCGGCTTAGCATCATTCTTA+GGG - chr5.1:67493501-67493520 None:intergenic 45.0%
CTACACATTGCAGCTAGGTA+TGG + chr5.1:67492962-67492981 MS.gene81617:CDS 45.0%
GATCTTGCAGCGAAATGCAA+AGG - chr5.1:67495315-67495334 None:intergenic 45.0%
GCAAGATGGAGTCTTACCAA+AGG + chr5.1:67492742-67492761 MS.gene81617:CDS 45.0%
GGCGTGATATAAAAGAGGCA+TGG + chr5.1:67493137-67493156 MS.gene81617:CDS 45.0%
GTGAACAAGGAGAATGCTCA+AGG + chr5.1:67493244-67493263 MS.gene81617:CDS 45.0%
TAAGAATGATGCTAAGCCGG+AGG + chr5.1:67493501-67493520 MS.gene81617:CDS 45.0%
TCAACAGGTGATAATCCCTC+CGG - chr5.1:67493520-67493539 None:intergenic 45.0%
TCCGGCTTAGCATCATTCTT+AGG - chr5.1:67493502-67493521 None:intergenic 45.0%
TCTTGCAGCGAAATGCAAAG+GGG - chr5.1:67495313-67495332 None:intergenic 45.0%
TGAGTGTCAAGCATCTCTGT+GGG - chr5.1:67495168-67495187 None:intergenic 45.0%
TTACATGTTGCTGCAAGAGG+TGG + chr5.1:67493064-67493083 MS.gene81617:CDS 45.0%
! ACAAGACTGTAGATCGGTTC+GGG + chr5.1:67493350-67493369 MS.gene81617:CDS 45.0%
! ATTTTTGCCTGGGTGTCAAG+AGG + chr5.1:67494839-67494858 MS.gene81617:intron 45.0%
! CCACGTATCAGATTTTTGCC+TGG + chr5.1:67494828-67494847 MS.gene81617:intron 45.0%
!! CAAGAGCCGTTTTGTTATCC+TGG - chr5.1:67495407-67495426 None:intergenic 45.0%
!! GCATATCACTTGGTGTATGG+AGG + chr5.1:67492875-67492894 MS.gene81617:CDS 45.0%
!! CTATAAAATTAAAATTAAAT+TGG - chr5.1:67493873-67493892 None:intergenic 5.0%
!!! AAATTAAAGTTATTTTTATA+AGG + chr5.1:67494336-67494355 MS.gene81617:intron 5.0%
!!! ATTTTACTAAAATATTATTA+TGG + chr5.1:67494605-67494624 MS.gene81617:intron 5.0%
!!! TCAAATATATTTATAATTTT+AGG - chr5.1:67492791-67492810 None:intergenic 5.0%
AGTGATCTCAGCGACTTCTG+AGG - chr5.1:67492816-67492835 None:intergenic 50.0%
CAATACACCTCTTGACACCC+AGG - chr5.1:67494849-67494868 None:intergenic 50.0%
CAGTGCTACACATTGCAGCT+AGG + chr5.1:67492957-67492976 MS.gene81617:CDS 50.0%
GCCTTACATGTTGCTGCAAG+AGG + chr5.1:67493061-67493080 MS.gene81617:CDS 50.0%
GTGATCTCAGCGACTTCTGA+GGG - chr5.1:67492815-67492834 None:intergenic 50.0%
TCAAGCATCTCTGTGGGATC+TGG - chr5.1:67495162-67495181 None:intergenic 50.0%
TTGTCGTCACACAAGCACGA+AGG + chr5.1:67493655-67493674 MS.gene81617:intron 50.0%
AGCATCTTCTGGTGGCCATG+TGG + chr5.1:67495107-67495126 MS.gene81617:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 67492698 67495518 67492698 ID=MS.gene81617
chr5.1 mRNA 67492698 67495518 67492698 ID=MS.gene81617.t1;Parent=MS.gene81617
chr5.1 exon 67492698 67493562 67492698 ID=MS.gene81617.t1.exon1;Parent=MS.gene81617.t1
chr5.1 CDS 67492698 67493562 67492698 ID=cds.MS.gene81617.t1;Parent=MS.gene81617.t1
chr5.1 exon 67495010 67495518 67495010 ID=MS.gene81617.t1.exon2;Parent=MS.gene81617.t1
chr5.1 CDS 67495010 67495518 67495010 ID=cds.MS.gene81617.t1;Parent=MS.gene81617.t1
Gene Sequence

>MS.gene81617

ATGGCTCCTAGTCTGGAGGAAAAAAAAAATTTAGACCAACTAGTGCAAGATGGAGTCTTACCAAAGGATTTTTCTAGTACTTATAAGGAACCTAAAATTATAAATATATTTGAACCCTCAGAAGTCGCTGAGATCACTCCCTGCGCATTTGACAATCAAGTTTATTTGCTTAGTGAAGCATATCACTTGGTGTATGGAGGTTCAACGAATTTGTTGGGTTCCTCTAATTTAATAGATATTGAAACCCCTACGAAAAATACAGTGCTACACATTGCAGCTAGGTATGGTAAAGATGACATTGTGAATCTTGTAATTGAACGTGGTCCAAAACTTTTGTTCAAATTTAACAAAAACAATGATAGCGCCTTACATGTTGCTGCAAGAGGTGGACATATCTCAACCGTTAAAAAGCTATTAGAAAATTATACTAATACTGAACGGCGTGATATAAAAGAGGCATGGTTAGAATACACTAACCGTTGGAGTGATGTAGAAGATGTAGAAGACTACGTTGAGGAGTTAAATATGGAGGACCTGTTATGTTTTGTGAACAAGGAGAATGCTCAAGGAAACACTATGTTACATGAAGCAATGTTATGTCGTGACATTAAGAGAATTGGCGGGGACAAGATTTTCAAAGTTTGTGAACTTTACAAGACTGTAGATCGGTTCGGGAATTCATTATCAGATTGTTGTTATGGATTTGCATTAGATATTGCTAATCTTACAAAGAAATCTGTAATTTACCTTGCGGTTGAAAATAAGGACAGGGATGCAGTCAAACTAATCTTGGATAATTGCCCTAAGAATGATGCTAAGCCGGAGGGATTATCACCTGTTGAAGCTATCATGAAGCATAATCACGGTACATTGGTCATAATATTATTGTTCAATCACTTGAATCATAACACTGAGAGTAAATATTTTATAGGATTATGCTTAAGTGCACGACACTATTTGTCGTCACACAAGCACGAAGGTCCTTACAATTTCTTTTATGATTTGGTTTTTTACTTTCTAAACTTTTTATTTCTTTTGTTTGTGTTTGTTGGTTTTATTTCATTTTTTAACTTACAAATATTTACAAACATTTTTTTTACAAATATTTACAAACATGATTACTTACCTGCATTTACAAATATGATTACTTACCTGCATTTTTTTTATGTTTTCCAATTTAATTTTAATTTTATAGATAAATATTTTATCAACAACATTAAAAATTTAATTGATCAAATTTATTTCAAATTAATATTGTATAATAAATTTAAGAACCATATTTTAGAGAGCGATAAATTTAGACCTACCAATTTAACAATAAACGTTCACATAAACACTTTTTAACATAGTTTGAATGAAAAATGTTAATGGTGCATACATTTTATGACTAATTAAAGGACTAAATACATACTTTTAGTGACATAAAGAAACAATTTAAAACTAAAAAAACTAAAATAACTAGTCCGATAAAAAAAAATTAAAGAACCCCATGATATTTTTGACCTTTTTTTTATAGGAACACAATGATCCAGATAAATTAAAAGAATTAAGGAAAGAAAATAAAATCAAAGCAAGTTAAATTACTTAAACGAATTGAATAAAACTATCTACAGATTTTTTTTTACTTAAAGAGCTACTAAATTAAAGTTATTTTTATAAGGCTAATGCATGCAAATGTGTTGTCGTGCTTTGTCGTGCCATATAGCACCGCTCTTTTTTTATTAGTCTCATGTTCTATTACTTTGAAAACTAAAATTAATATTGTCTAAATAAAGATTGAATTTAAATGATTTTACTATCATTATTAATTAAAAAAGAAATATTAAGCAAAATTGATATCCTTAGAATTCAATTGATGATTGTATATTCTAAACCGTTTGAAATTTGTCATTTCAAGAAAATTACTTATTTTACTAAAATATTATTATGGTCTTCAATAAAAGACACCATCGTAAATTTGTATTTCGCTTCAATACTTGTTGAACTTCCCCATACAATTAGGGTACTTGTGACAAAATGGGTAATATTTAGGAAAATGTTACATAGACACATTTTATTCCTATACACTTTTGTACACCTCATACATTAGGAAGAGAGAAGAGGAGATGAAAGAGAAAGTGTGCTTGGGGTCCACGTATCAGATTTTTGCCTGGGTGTCAAGAGGTGTATTGTCACCTATGGTTGTTTATGTATCATAACTCTAATATTTATGTTTGTGTGTCAGAAATGCTCAGCACCATACTACAAAACAAACCAACTTGGATCCATTTAAGGAACAAATATGGAAGACTTCCTCTTCATTACGCATGCAGCATCTATAGGTTACCTTGAAGGTGTTGAATTATTACTTGGCATGTGCAAATGCTGCACAATTCAAAGTGACATTGATGGCTCTTTTCCAATTCATAAAGCATCTTCTGGTGGCCATGTGGAAGTAGTGAAGAAGTTGCTAGAATATTGTCCAGATCCCACAGAGATGCTTGACACTCATGAACGTAATATTCTTCACATTGCATCAATCAATGGAAAACATGATGTCATACGCTACATACAAGAAAATCAAGTTCATGGACACCGTAAGATGATAAATCAGAAAGATAGGAATGGAGACACCCCTTTGCATTTCGCTGCAAGATCATGTCATCCCATGACTGTGTTCTACTTAGTTAACCAAAACAACGAAAAAGTGCATCTTGATATGGTTAACCAGGATAACAAAACGGCTCTTGACATTGTTATTTCACTTTACGAGCATGATAAATCATCTTTGAGACAGGCAAGTAGTTCTATCCCCTCGCAAACTCACAAATTTATAGGTTAA
Protein sequence

>MS.gene81617.t1

MAPSLEEKKNLDQLVQDGVLPKDFSSTYKEPKIINIFEPSEVAEITPCAFDNQVYLLSEAYHLVYGGSTNLLGSSNLIDIETPTKNTVLHIAARYGKDDIVNLVIERGPKLLFKFNKNNDSALHVAARGGHISTVKKLLENYTNTERRDIKEAWLEYTNRWSDVEDVEDYVEELNMEDLLCFVNKENAQGNTMLHEAMLCRDIKRIGGDKIFKVCELYKTVDRFGNSLSDCCYGFALDIANLTKKSVIYLAVENKDRDAVKLILDNCPKNDAKPEGLSPVEAIMKHNHASIGYLEGVELLLGMCKCCTIQSDIDGSFPIHKASSGGHVEVVKKLLEYCPDPTEMLDTHERNILHIASINGKHDVIRYIQENQVHGHRKMINQKDRNGDTPLHFAARSCHPMTVFYLVNQNNEKVHLDMVNQDNKTALDIVISLYEHDKSSLRQASSSIPSQTHKFIG