AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057886

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057886.t1 MTR_2g033050 81.795 390 67 3 75 461 1 389 0.0 646
MS.gene057886.t1 MTR_5g085480 72.897 428 112 3 28 454 2 426 0.0 625
MS.gene057886.t1 MTR_5g085830 61.050 457 158 8 4 456 21 461 0.0 535
MS.gene057886.t1 MTR_5g085810 58.696 460 152 7 27 456 36 487 3.60e-177 514
MS.gene057886.t1 MTR_5g085490 72.727 286 74 3 2 285 9 292 1.02e-140 405
MS.gene057886.t1 MTR_5g085480 74.157 267 68 1 188 454 1 266 1.27e-138 409
MS.gene057886.t1 MTR_5g085500 88.199 161 19 0 301 461 2 162 1.29e-97 301
MS.gene057886.t1 MTR_5g085520 70.202 198 57 2 82 277 1 198 2.25e-87 266
MS.gene057886.t1 MTR_5g085440 82.468 154 27 0 2 155 3 156 1.61e-86 270
MS.gene057886.t1 MTR_5g085530 82.192 73 12 1 279 350 1 73 4.41e-35 125
MS.gene057886.t1 MTR_1g069110 34.239 184 116 4 286 467 30 210 8.35e-18 85.9
MS.gene057886.t1 MTR_7g100390 30.180 222 145 5 235 453 46 260 2.11e-13 72.8
MS.gene057886.t1 MTR_2g101310 28.261 184 115 5 277 454 145 317 2.12e-12 69.7
MS.gene057886.t1 MTR_2g096190 28.507 221 148 4 235 452 63 276 2.23e-12 69.3
MS.gene057886.t1 MTR_2g101310 28.261 184 115 5 277 454 145 317 3.01e-12 68.9
MS.gene057886.t1 MTR_4g062100 28.378 222 147 6 235 452 67 280 1.54e-11 67.0
MS.gene057886.t1 MTR_2g101320 27.222 180 122 4 277 454 145 317 2.95e-11 66.2
MS.gene057886.t1 MTR_1g032100 27.848 237 160 5 228 461 47 275 4.81e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057886.t1 AT4G03500 31.267 371 208 10 84 453 103 427 5.79e-37 144
MS.gene057886.t1 AT4G03500 31.267 371 208 10 84 453 80 404 6.53e-37 144
MS.gene057886.t1 AT4G03500 31.267 371 208 10 84 453 80 404 6.53e-37 144
MS.gene057886.t1 AT4G03480 29.508 427 237 14 75 461 142 544 1.02e-33 134
MS.gene057886.t1 AT4G03480 29.302 430 237 14 75 461 142 547 3.88e-33 133
MS.gene057886.t1 AT1G03670 29.101 378 207 10 84 453 73 397 4.08e-33 132
MS.gene057886.t1 AT4G03440 30.732 410 238 12 79 461 114 504 1.15e-32 132
MS.gene057886.t1 AT4G03440 30.583 412 236 13 79 461 107 497 1.34e-32 132
MS.gene057886.t1 AT4G03440 30.583 412 236 13 79 461 107 497 1.34e-32 132
MS.gene057886.t1 AT4G05040 29.723 397 217 10 75 454 100 451 2.08e-31 127
MS.gene057886.t1 AT4G05040 29.723 397 217 10 75 454 100 451 2.08e-31 127
MS.gene057886.t1 AT4G05040 29.723 397 217 10 75 454 100 451 2.08e-31 127
MS.gene057886.t1 AT4G05040 29.723 397 217 10 75 454 100 451 2.08e-31 127
MS.gene057886.t1 AT4G05040 29.723 397 217 10 75 454 100 451 2.08e-31 127
MS.gene057886.t1 AT4G05040 29.723 397 217 10 75 454 100 451 2.08e-31 127
MS.gene057886.t1 AT4G05040 29.723 397 217 10 75 454 100 451 2.08e-31 127
MS.gene057886.t1 AT4G03460 35.573 253 146 7 226 469 148 392 9.17e-31 125
MS.gene057886.t1 AT4G03460 35.573 253 146 7 226 469 180 424 1.02e-30 125
MS.gene057886.t1 AT4G03490 28.535 396 211 12 84 452 37 387 1.62e-30 125
MS.gene057886.t1 AT4G03490 28.535 396 211 12 84 452 27 377 1.85e-30 125
MS.gene057886.t1 AT4G03490 28.535 396 211 12 84 452 37 387 2.54e-30 124
MS.gene057886.t1 AT4G03470 28.101 395 221 10 75 459 111 452 1.05e-27 117
MS.gene057886.t1 AT4G14390 27.692 390 227 10 74 453 123 467 1.40e-27 116
MS.gene057886.t1 AT4G14390 27.692 390 227 10 74 453 104 448 1.42e-27 115
MS.gene057886.t1 AT4G14390 27.692 390 227 10 74 453 104 448 1.48e-27 116
MS.gene057886.t1 AT4G03490 28.261 368 194 11 84 424 27 351 3.42e-27 115
MS.gene057886.t1 AT4G03490 28.261 368 194 11 84 424 17 341 4.33e-27 115
MS.gene057886.t1 AT4G14390 27.621 391 227 10 74 453 123 468 4.82e-27 115
MS.gene057886.t1 AT4G03450 28.165 387 228 13 87 468 75 416 5.50e-25 108
MS.gene057886.t1 AT4G03450 28.495 372 220 12 87 453 75 405 7.85e-25 108
MS.gene057886.t1 AT4G14400 27.665 394 221 11 80 453 91 440 7.66e-24 105
MS.gene057886.t1 AT4G14400 27.665 394 221 11 80 453 25 374 1.10e-23 104
MS.gene057886.t1 AT4G14400 27.665 394 221 11 80 453 25 374 1.10e-23 104
MS.gene057886.t1 AT5G02620 29.596 223 145 6 235 453 53 267 1.03e-14 77.0
MS.gene057886.t1 AT5G02620 29.596 223 145 6 235 453 53 267 1.03e-14 77.0
MS.gene057886.t1 AT5G02620 29.596 223 145 6 235 453 53 267 1.03e-14 77.0
MS.gene057886.t1 AT5G60070 27.928 222 151 4 235 452 70 286 4.59e-14 74.7
MS.gene057886.t1 AT5G02620 27.869 244 145 8 235 453 53 290 2.27e-12 69.7
MS.gene057886.t1 AT5G02620 27.869 244 145 8 235 453 53 290 2.27e-12 69.7
MS.gene057886.t1 AT2G01680 25.221 226 159 4 231 453 52 270 4.10e-12 68.6
MS.gene057886.t1 AT2G31820 29.167 216 141 6 242 453 193 400 2.11e-11 66.6
MS.gene057886.t1 AT5G50140 23.529 187 121 4 277 448 34 213 9.46e-11 64.3

Find 91 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTCTTCATCATACTCTTC+TGG 0.146198 5.3:-69228869 None:intergenic
AATTAATTTGATTGTCAAAT+TGG 0.185555 5.3:-69228523 None:intergenic
TTGGACAGCATGATATAAAA+AGG 0.215429 5.3:+69228813 MS.gene057886:CDS
TTCGGCTTAGCATCATTCTT+AGG 0.232416 5.3:-69229169 None:intergenic
CAAGAGCTGTTTCATTGTTT+TGG 0.257414 5.3:-69230216 None:intergenic
ATTGTCAAATTGGCAACATT+TGG 0.262468 5.3:-69228513 None:intergenic
CCATATGATGCTAGATGAAT+TGG 0.269046 5.3:-69229905 None:intergenic
TTAAATTTGAACAAAAGTTT+TGG 0.280811 5.3:-69228704 None:intergenic
GTTTCACTCTTTCTTTGCTT+TGG 0.290699 5.3:-69230180 None:intergenic
TTAGCCAGTTATACTAATAT+TGG 0.290809 5.3:+69228794 MS.gene057886:CDS
CTGTCCAATATTAGTATAAC+TGG 0.292747 5.3:-69228798 None:intergenic
CTTCACATTGCATCAAACTA+TGG 0.318717 5.3:+69230013 MS.gene057886:CDS
AGAAAGAGTGAAACTTGATT+TGG 0.333662 5.3:+69230189 MS.gene057886:CDS
GTATGATGAAGAGTCAAATA+TGG 0.336358 5.3:+69228877 MS.gene057886:CDS
ACAATTCAAAGGGACAAATA+TGG 0.343812 5.3:+69229875 MS.gene057886:CDS
CATTGTTACTGCACCTTTCG+AGG 0.345501 5.3:+69230240 MS.gene057886:CDS
ACTCTTCATCATACTCTTCT+GGG 0.351775 5.3:-69228868 None:intergenic
TGGAGACACCCCTTTGCATT+TGG 0.361651 5.3:+69230114 MS.gene057886:CDS
CTTCATTACGCATCACCTAT+AGG 0.368319 5.3:+69229806 MS.gene057886:CDS
TTTCATGTTTGTCCCTTGAA+TGG 0.376767 5.3:-69229775 None:intergenic
TGGAGTTTCAACAAATTTGT+TGG 0.381612 5.3:+69228574 MS.gene057886:CDS
GAAGCATATCAATTGGTCTA+TGG 0.390920 5.3:+69228554 MS.gene057886:CDS
CCAATTCATCTAGCATCATA+TGG 0.404047 5.3:+69229905 MS.gene057886:CDS
TTGTCAAATTGGCAACATTT+GGG 0.406024 5.3:-69228512 None:intergenic
GAGGGTTCAATTTCAAGTTC+AGG 0.407035 5.3:-69228470 None:intergenic
AGATGATAAATCAGAAAGAT+AGG 0.412813 5.3:+69230089 MS.gene057886:CDS
TCGGCTTAGCATCATTCTTA+GGG 0.419240 5.3:-69229168 None:intergenic
GCTTAATGAAGCATATCAAT+TGG 0.419437 5.3:+69228547 MS.gene057886:CDS
TACAAAACTGAAGATTTGTC+TGG 0.424015 5.3:+69229019 MS.gene057886:CDS
CTTCATGATAGCTGCTACAA+CGG 0.437012 5.3:-69229203 None:intergenic
TATTAATCATGCAAAGAAAT+CGG 0.439896 5.3:+69229084 MS.gene057886:CDS
GTAATAATTCAACACCTTCA+AGG 0.447605 5.3:-69229832 None:intergenic
AGATTTGTCTGGATACTCAT+TGG 0.451663 5.3:+69229030 MS.gene057886:CDS
TCAAGCATTTCTGTGGGATC+AGG 0.452092 5.3:-69229968 None:intergenic
GTGACAAGAGCAATATGAGC+AGG 0.467735 5.3:+69228969 MS.gene057886:CDS
ATCTTGCAGCCAAATGCAAA+GGG 0.474929 5.3:-69230123 None:intergenic
AAGGAATGTTAGTAGTGAAA+AGG 0.480134 5.3:+69228445 MS.gene057886:CDS
ACCTCTTGCAGCAACATGTA+AGG 0.481999 5.3:-69228744 None:intergenic
ACCGTTGAAGAACACTTTAG+AGG 0.488199 5.3:-69230460 None:intergenic
GTGAAGGAGAAGAATGTTCA+AGG 0.492369 5.3:+69228917 MS.gene057886:CDS
TTCATGATAGCTGCTACAAC+GGG 0.494572 5.3:-69229202 None:intergenic
ACTACTAACATTCCTTGATA+CGG 0.495639 5.3:-69228438 None:intergenic
ACACCTTCAAGGTAACCTAT+AGG 0.496942 5.3:-69229821 None:intergenic
TGCAAGATGAAGCCGTATCA+AGG 0.505158 5.3:+69228426 MS.gene057886:CDS
TACTAGAAAACAAACCAACT+TGG 0.508083 5.3:+69229750 MS.gene057886:CDS
GCAAATGCTGCACAATTCAA+AGG 0.508451 5.3:+69229864 MS.gene057886:CDS
AACCAACTTGGATCCATTCA+AGG 0.510363 5.3:+69229762 MS.gene057886:CDS
TCCCTTGAATGGATCCAAGT+TGG 0.516799 5.3:-69229764 None:intergenic
GCATCAAACTATGGAAAACA+TGG 0.519449 5.3:+69230022 MS.gene057886:CDS
ACTAAGTAGTACACAGTTGT+GGG 0.522745 5.3:-69230154 None:intergenic
TCACCTATAGGTTACCTTGA+AGG 0.526767 5.3:+69229818 MS.gene057886:CDS
CTACATATTGCAGCTTGGTA+TGG 0.527273 5.3:+69228644 MS.gene057886:CDS
TTACCTTGCGGTCGAAAATG+AGG 0.528431 5.3:+69229111 MS.gene057886:CDS
CATATGATGCTAGATGAATT+GGG 0.531667 5.3:-69229904 None:intergenic
GGACTTGTTAAAATTTGTGA+AGG 0.531768 5.3:+69228901 MS.gene057886:CDS
GATACGGCTTCATCTTGCAA+TGG 0.537281 5.3:-69228422 None:intergenic
CAAATGCTGCACAATTCAAA+GGG 0.544446 5.3:+69229865 MS.gene057886:CDS
AACTAAGTAGTACACAGTTG+TGG 0.547444 5.3:-69230155 None:intergenic
GCTATCATGAAGCAGAATCA+AGG 0.549976 5.3:+69229214 MS.gene057886:CDS
ACCTCTAAAGTGTTCTTCAA+CGG 0.558733 5.3:+69230459 MS.gene057886:CDS
AATGACCTCAATGACTTCTG+AGG 0.562524 5.3:-69228489 None:intergenic
ATAAATCAGAAAGATAGGAA+TGG 0.570805 5.3:+69230094 MS.gene057886:CDS
GATCTTGCAGCCAAATGCAA+AGG 0.573294 5.3:-69230124 None:intergenic
CATCAAACTATGGAAAACAT+GGG 0.575809 5.3:+69230023 MS.gene057886:CDS
GATGATTTATCAACCTCGAA+AGG 0.576087 5.3:-69230253 None:intergenic
AGAAGCGTCAAGCATTTCTG+TGG 0.600433 5.3:-69229975 None:intergenic
ATGACCTCAATGACTTCTGA+GGG 0.600511 5.3:-69228488 None:intergenic
TAAGAATGATGCTAAGCCGA+AGG 0.600924 5.3:+69229171 MS.gene057886:CDS
TATAATATGCCTCATCCAGA+GGG 0.602495 5.3:+69228380 None:intergenic
GCCTTACATGTTGCTGCAAG+AGG 0.610873 5.3:+69228743 MS.gene057886:CDS
AAGAATGATGCTAAGCCGAA+GGG 0.615867 5.3:+69229172 MS.gene057886:CDS
TGAACCCTCAGAAGTCATTG+AGG 0.619198 5.3:+69228484 MS.gene057886:CDS
TGACAAGAGCAATATGAGCA+GGG 0.620460 5.3:+69228970 MS.gene057886:CDS
ACAACGGGCGATAATCCCTT+CGG 0.620725 5.3:-69229187 None:intergenic
TGCGGTCGAAAATGAGGACA+AGG 0.624751 5.3:+69229117 MS.gene057886:CDS
ATAGGTGATGCGTAATGAAG+AGG 0.629392 5.3:-69229803 None:intergenic
ATTCAAGGGACAAACATGAA+AGG 0.633833 5.3:+69229777 MS.gene057886:CDS
TTACATGTTGCTGCAAGAGG+TGG 0.637643 5.3:+69228746 MS.gene057886:CDS
ACCAACTTGGATCCATTCAA+GGG 0.638851 5.3:+69229763 MS.gene057886:CDS
CAGTGCTACATATTGCAGCT+TGG 0.640810 5.3:+69228639 MS.gene057886:CDS
GAAGCGTCAAGCATTTCTGT+GGG 0.648056 5.3:-69229974 None:intergenic
AGCATCATATGGTGGCCATG+TGG 0.648207 5.3:+69229916 MS.gene057886:CDS
TTATAATATGCCTCATCCAG+AGG 0.656293 5.3:+69228379 None:intergenic
AAACTATGGAAAACATGGGG+TGG 0.657037 5.3:+69230027 MS.gene057886:CDS
TGATGAAGAGTCAAATATGG+AGG 0.657104 5.3:+69228880 MS.gene057886:CDS
ACTTCTTCACTACTTCCACA+TGG 0.679088 5.3:-69229931 None:intergenic
GAAATCGGTACTTTACCTTG+CGG 0.683273 5.3:+69229099 MS.gene057886:CDS
AGGCAACACTATGTTACGTG+AGG 0.692488 5.3:+69228937 MS.gene057886:CDS
ATTCATCTAGCATCATATGG+TGG 0.697537 5.3:+69229908 MS.gene057886:CDS
ATCAAACTATGGAAAACATG+GGG 0.709457 5.3:+69230024 MS.gene057886:CDS
TCTTGCAGCCAAATGCAAAG+GGG 0.746892 5.3:-69230122 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAAAAAAATTAATTTTA+GGG + chr5.3:69230426-69230445 MS.gene057886:intron 0.0%
!!! TTAAAAAAAAAATTAATTTT+AGG + chr5.3:69230425-69230444 MS.gene057886:intron 0.0%
!! TATAAGTAAATCTAAATCAA+TGG + chr5.3:69230355-69230374 MS.gene057886:intron 15.0%
!!! AATTAATTTGATTGTCAAAT+TGG - chr5.3:69228526-69228545 None:intergenic 15.0%
!!! TTAAATTTGAACAAAAGTTT+TGG - chr5.3:69228707-69228726 None:intergenic 15.0%
!! TATTAATCATGCAAAGAAAT+CGG + chr5.3:69229084-69229103 MS.gene057886:CDS 20.0%
! AGATGATAAATCAGAAAGAT+AGG + chr5.3:69230089-69230108 MS.gene057886:CDS 25.0%
! AGTGAATGATTTATAACCTT+TGG - chr5.3:69229374-69229393 None:intergenic 25.0%
! ATAAATCAGAAAGATAGGAA+TGG + chr5.3:69230094-69230113 MS.gene057886:CDS 25.0%
! TTAGCCAGTTATACTAATAT+TGG + chr5.3:69228794-69228813 MS.gene057886:CDS 25.0%
!! CTAATAGCTTTTTAACAGTT+GGG - chr5.3:69228778-69228797 None:intergenic 25.0%
AACAAAAATTGAGACTCCTA+TGG + chr5.3:69228610-69228629 MS.gene057886:CDS 30.0%
AAGGAATGTTAGTAGTGAAA+AGG + chr5.3:69228445-69228464 MS.gene057886:CDS 30.0%
ACAATTCAAAGGGACAAATA+TGG + chr5.3:69229875-69229894 MS.gene057886:CDS 30.0%
ACTACTAACATTCCTTGATA+CGG - chr5.3:69228441-69228460 None:intergenic 30.0%
AGAAAGAGTGAAACTTGATT+TGG + chr5.3:69230189-69230208 MS.gene057886:CDS 30.0%
AGCATGATATAAAAAGGGAA+TGG + chr5.3:69228819-69228838 MS.gene057886:CDS 30.0%
ATCAAACTATGGAAAACATG+GGG + chr5.3:69230024-69230043 MS.gene057886:CDS 30.0%
ATTGTCAAATTGGCAACATT+TGG - chr5.3:69228516-69228535 None:intergenic 30.0%
CATATGATGCTAGATGAATT+GGG - chr5.3:69229907-69229926 None:intergenic 30.0%
CATCAAACTATGGAAAACAT+GGG + chr5.3:69230023-69230042 MS.gene057886:CDS 30.0%
CATCATCGTAAATTTGTCTT+AGG + chr5.3:69229635-69229654 MS.gene057886:intron 30.0%
CTGTCCAATATTAGTATAAC+TGG - chr5.3:69228801-69228820 None:intergenic 30.0%
GAATGATTTATAACCTTTGG+AGG - chr5.3:69229371-69229390 None:intergenic 30.0%
GCTTAATGAAGCATATCAAT+TGG + chr5.3:69228547-69228566 MS.gene057886:CDS 30.0%
GTAATAATTCAACACCTTCA+AGG - chr5.3:69229835-69229854 None:intergenic 30.0%
GTATGATGAAGAGTCAAATA+TGG + chr5.3:69228877-69228896 MS.gene057886:CDS 30.0%
TACAAAACTGAAGATTTGTC+TGG + chr5.3:69229019-69229038 MS.gene057886:CDS 30.0%
TACTAGAAAACAAACCAACT+TGG + chr5.3:69229750-69229769 MS.gene057886:CDS 30.0%
TGGACAGCATGATATAAAAA+GGG + chr5.3:69228814-69228833 MS.gene057886:CDS 30.0%
TGGAGTTTCAACAAATTTGT+TGG + chr5.3:69228574-69228593 MS.gene057886:CDS 30.0%
TTGGACAGCATGATATAAAA+AGG + chr5.3:69228813-69228832 MS.gene057886:CDS 30.0%
TTGTCAAATTGGCAACATTT+GGG - chr5.3:69228515-69228534 None:intergenic 30.0%
! GCAAAAAATAATTTTCCCTC+TGG - chr5.3:69228398-69228417 None:intergenic 30.0%
! GCTAATAGCTTTTTAACAGT+TGG - chr5.3:69228779-69228798 None:intergenic 30.0%
!! GGACTTGTTAAAATTTGTGA+AGG + chr5.3:69228901-69228920 MS.gene057886:CDS 30.0%
!! TGATAAATCATCTTTGAGAC+AGG + chr5.3:69230264-69230283 MS.gene057886:intron 30.0%
!!! AGTTGGTTTGTTTTCTAGTA+TGG - chr5.3:69229750-69229769 None:intergenic 30.0%
AACTAAGTAGTACACAGTTG+TGG - chr5.3:69230158-69230177 None:intergenic 35.0%
ACTAAGTAGTACACAGTTGT+GGG - chr5.3:69230157-69230176 None:intergenic 35.0%
ACTCTTCATCATACTCTTCT+GGG - chr5.3:69228871-69228890 None:intergenic 35.0%
AGATTTGTCTGGATACTCAT+TGG + chr5.3:69229030-69229049 MS.gene057886:CDS 35.0%
ATTCAAGGGACAAACATGAA+AGG + chr5.3:69229777-69229796 MS.gene057886:CDS 35.0%
ATTCATCTAGCATCATATGG+TGG + chr5.3:69229908-69229927 MS.gene057886:CDS 35.0%
CAAATGCTGCACAATTCAAA+GGG + chr5.3:69229865-69229884 MS.gene057886:CDS 35.0%
CCAATTCATCTAGCATCATA+TGG + chr5.3:69229905-69229924 MS.gene057886:CDS 35.0%
CCATATGATGCTAGATGAAT+TGG - chr5.3:69229908-69229927 None:intergenic 35.0%
CTTCACATTGCATCAAACTA+TGG + chr5.3:69230013-69230032 MS.gene057886:CDS 35.0%
GATGATTTATCAACCTCGAA+AGG - chr5.3:69230256-69230275 None:intergenic 35.0%
GCATCAAACTATGGAAAACA+TGG + chr5.3:69230022-69230041 MS.gene057886:CDS 35.0%
GTTTCACTCTTTCTTTGCTT+TGG - chr5.3:69230183-69230202 None:intergenic 35.0%
TCATGCAAATTGTCATCATG+AGG + chr5.3:69229301-69229320 MS.gene057886:intron 35.0%
TGATGAAGAGTCAAATATGG+AGG + chr5.3:69228880-69228899 MS.gene057886:CDS 35.0%
TTTCATGTTTGTCCCTTGAA+TGG - chr5.3:69229778-69229797 None:intergenic 35.0%
! AATAATTTTCCCTCTGGATG+AGG - chr5.3:69228392-69228411 None:intergenic 35.0%
! GAAGCATATCAATTGGTCTA+TGG + chr5.3:69228554-69228573 MS.gene057886:CDS 35.0%
! GTATTGTTGACCTTACCTTT+TGG - chr5.3:69229336-69229355 None:intergenic 35.0%
!! ACCTCTAAAGTGTTCTTCAA+CGG + chr5.3:69230459-69230478 MS.gene057886:CDS 35.0%
!! CAAGAGCTGTTTCATTGTTT+TGG - chr5.3:69230219-69230238 None:intergenic 35.0%
AAACTATGGAAAACATGGGG+TGG + chr5.3:69230027-69230046 MS.gene057886:CDS 40.0%
AACCAACTTGGATCCATTCA+AGG + chr5.3:69229762-69229781 MS.gene057886:CDS 40.0%
AAGAATGATGCTAAGCCGAA+GGG + chr5.3:69229172-69229191 MS.gene057886:CDS 40.0%
AATGACCTCAATGACTTCTG+AGG - chr5.3:69228492-69228511 None:intergenic 40.0%
ACACCTTCAAGGTAACCTAT+AGG - chr5.3:69229824-69229843 None:intergenic 40.0%
ACCAACTTGGATCCATTCAA+GGG + chr5.3:69229763-69229782 MS.gene057886:CDS 40.0%
ACCGTTGAAGAACACTTTAG+AGG - chr5.3:69230463-69230482 None:intergenic 40.0%
ACTTCTTCACTACTTCCACA+TGG - chr5.3:69229934-69229953 None:intergenic 40.0%
ATAGGTGATGCGTAATGAAG+AGG - chr5.3:69229806-69229825 None:intergenic 40.0%
ATCTTGCAGCCAAATGCAAA+GGG - chr5.3:69230126-69230145 None:intergenic 40.0%
ATGACCTCAATGACTTCTGA+GGG - chr5.3:69228491-69228510 None:intergenic 40.0%
CACAAGCACCCTAATAGTAT+GGG - chr5.3:69229682-69229701 None:intergenic 40.0%
CTACATATTGCAGCTTGGTA+TGG + chr5.3:69228644-69228663 MS.gene057886:CDS 40.0%
CTTCATGATAGCTGCTACAA+CGG - chr5.3:69229206-69229225 None:intergenic 40.0%
CTTCATTACGCATCACCTAT+AGG + chr5.3:69229806-69229825 MS.gene057886:CDS 40.0%
GAAATCGGTACTTTACCTTG+CGG + chr5.3:69229099-69229118 MS.gene057886:CDS 40.0%
GACTCTTCATCATACTCTTC+TGG - chr5.3:69228872-69228891 None:intergenic 40.0%
GCAAATGCTGCACAATTCAA+AGG + chr5.3:69229864-69229883 MS.gene057886:CDS 40.0%
GCTATCATGAAGCAGAATCA+AGG + chr5.3:69229214-69229233 MS.gene057886:CDS 40.0%
GTGAAGGAGAAGAATGTTCA+AGG + chr5.3:69228917-69228936 MS.gene057886:CDS 40.0%
TAAGAATGATGCTAAGCCGA+AGG + chr5.3:69229171-69229190 MS.gene057886:CDS 40.0%
TCACAAGCACCCTAATAGTA+TGG - chr5.3:69229683-69229702 None:intergenic 40.0%
TCACCTATAGGTTACCTTGA+AGG + chr5.3:69229818-69229837 MS.gene057886:CDS 40.0%
TCGGCTTAGCATCATTCTTA+GGG - chr5.3:69229171-69229190 None:intergenic 40.0%
TGACAAGAGCAATATGAGCA+GGG + chr5.3:69228970-69228989 MS.gene057886:CDS 40.0%
TTCATGATAGCTGCTACAAC+GGG - chr5.3:69229205-69229224 None:intergenic 40.0%
TTCGGCTTAGCATCATTCTT+AGG - chr5.3:69229172-69229191 None:intergenic 40.0%
! TAGGGTGCTTGTGACAAAAT+GGG + chr5.3:69229689-69229708 MS.gene057886:intron 40.0%
! TGTAGCACTGTGTTTTCCAT+AGG - chr5.3:69228629-69228648 None:intergenic 40.0%
! TGTGCTGACCCATACTATTA+GGG + chr5.3:69229671-69229690 MS.gene057886:intron 40.0%
! TTAGGGTGCTTGTGACAAAA+TGG + chr5.3:69229688-69229707 MS.gene057886:intron 40.0%
! TTGTGCTGACCCATACTATT+AGG + chr5.3:69229670-69229689 MS.gene057886:intron 40.0%
!! CAATAGTCTTATGCCTCCAA+AGG + chr5.3:69229355-69229374 MS.gene057886:intron 40.0%
!! GAGGGTTCAATTTCAAGTTC+AGG - chr5.3:69228473-69228492 None:intergenic 40.0%
ACCTCTTGCAGCAACATGTA+AGG - chr5.3:69228747-69228766 None:intergenic 45.0%
AGGCAACACTATGTTACGTG+AGG + chr5.3:69228937-69228956 MS.gene057886:CDS 45.0%
ATTGTCATCATGAGGCAGGA+CGG + chr5.3:69229309-69229328 MS.gene057886:intron 45.0%
CAGTGCTACATATTGCAGCT+TGG + chr5.3:69228639-69228658 MS.gene057886:CDS 45.0%
CATTGTTACTGCACCTTTCG+AGG + chr5.3:69230240-69230259 MS.gene057886:CDS 45.0%
GATACGGCTTCATCTTGCAA+TGG - chr5.3:69228425-69228444 None:intergenic 45.0%
GATCTTGCAGCCAAATGCAA+AGG - chr5.3:69230127-69230146 None:intergenic 45.0%
GCAAATTGTCATCATGAGGC+AGG + chr5.3:69229305-69229324 MS.gene057886:intron 45.0%
GTGACAAGAGCAATATGAGC+AGG + chr5.3:69228969-69228988 MS.gene057886:CDS 45.0%
TCAAGCATTTCTGTGGGATC+AGG - chr5.3:69229971-69229990 None:intergenic 45.0%
TCCCTTGAATGGATCCAAGT+TGG - chr5.3:69229767-69229786 None:intergenic 45.0%
TCTTGCAGCCAAATGCAAAG+GGG - chr5.3:69230125-69230144 None:intergenic 45.0%
TGAACCCTCAGAAGTCATTG+AGG + chr5.3:69228484-69228503 MS.gene057886:CDS 45.0%
TGCAAGATGAAGCCGTATCA+AGG + chr5.3:69228426-69228445 MS.gene057886:CDS 45.0%
TTACATGTTGCTGCAAGAGG+TGG + chr5.3:69228746-69228765 MS.gene057886:CDS 45.0%
TTACCTTGCGGTCGAAAATG+AGG + chr5.3:69229111-69229130 MS.gene057886:CDS 45.0%
! GAAGCGTCAAGCATTTCTGT+GGG - chr5.3:69229977-69229996 None:intergenic 45.0%
!! AGAAGCGTCAAGCATTTCTG+TGG - chr5.3:69229978-69229997 None:intergenic 45.0%
ACAACGGGCGATAATCCCTT+CGG - chr5.3:69229190-69229209 None:intergenic 50.0%
AGCATCATATGGTGGCCATG+TGG + chr5.3:69229916-69229935 MS.gene057886:CDS 50.0%
GCCTTACATGTTGCTGCAAG+AGG + chr5.3:69228743-69228762 MS.gene057886:CDS 50.0%
TGCGGTCGAAAATGAGGACA+AGG + chr5.3:69229117-69229136 MS.gene057886:CDS 50.0%
TGGAGACACCCCTTTGCATT+TGG + chr5.3:69230114-69230133 MS.gene057886:CDS 50.0%
! TGTCCTCATTTTCGACCGCA+AGG - chr5.3:69229117-69229136 None:intergenic 50.0%
ATGAGGCAGGACGGTCCAAA+AGG + chr5.3:69229318-69229337 MS.gene057886:intron 55.0%
GCAGGACGGTCCAAAAGGTA+AGG + chr5.3:69229323-69229342 MS.gene057886:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 69228386 69230488 69228386 ID=MS.gene057886
chr5.3 mRNA 69228386 69230488 69228386 ID=MS.gene057886.t1;Parent=MS.gene057886
chr5.3 exon 69228386 69229235 69228386 ID=MS.gene057886.t1.exon1;Parent=MS.gene057886.t1
chr5.3 CDS 69228386 69229235 69228386 ID=cds.MS.gene057886.t1;Parent=MS.gene057886.t1
chr5.3 exon 69229735 69230261 69229735 ID=MS.gene057886.t1.exon2;Parent=MS.gene057886.t1
chr5.3 CDS 69229735 69230261 69229735 ID=cds.MS.gene057886.t1;Parent=MS.gene057886.t1
chr5.3 exon 69230447 69230488 69230447 ID=MS.gene057886.t1.exon3;Parent=MS.gene057886.t1
chr5.3 CDS 69230447 69230488 69230447 ID=cds.MS.gene057886.t1;Parent=MS.gene057886.t1
Gene Sequence

>MS.gene057886

ATGCCTCATCCAGAGGGAAAATTATTTTTTGCTGAACCATTGCAAGATGAAGCCGTATCAAGGAATGTTAGTAGTGAAAAGGAGCCTGAACTTGAAATTGAACCCTCAGAAGTCATTGAGGTCATTCCCAAATGTTGCCAATTTGACAATCAAATTAATTTGCTTAATGAAGCATATCAATTGGTCTATGGAGTTTCAACAAATTTGTTGGATTTCTCTAATTTAACAAAAATTGAGACTCCTATGGAAAACACAGTGCTACATATTGCAGCTTGGTATGGTAATAATGACATTGTGAATCTTGTAATTCAACATGCTCCAAAACTTTTGTTCAAATTTAACAAAAACTATGATAGTGCCTTACATGTTGCTGCAAGAGGTGGACACATCCCAACTGTTAAAAAGCTATTAGCCAGTTATACTAATATTGGACAGCATGATATAAAAAGGGAATGGTTAGAATACACTAAAAATAATGATGACCCAGAAGAGTATGATGAAGAGTCAAATATGGAGGACTTGTTAAAATTTGTGAAGGAGAAGAATGTTCAAGGCAACACTATGTTACGTGAGGCAATGTTAAGTGACAAGAGCAATATGAGCAGGGATATGATTTTCATAGTTTGTGAACTTTACAAAACTGAAGATTTGTCTGGATACTCATTGGCAAATTCTTGTTATGAATTTGCGTTAGACATTATTAATCATGCAAAGAAATCGGTACTTTACCTTGCGGTCGAAAATGAGGACAAGGATGCAGTCAAACTAATCTTGAAGAATAGCCCTAAGAATGATGCTAAGCCGAAGGGATTATCGCCCGTTGTAGCAGCTATCATGAAGCAGAATCAAGGTATGTAAGTTGATCATAATATTGTTGTTTAATCACTTGAATGATAACATTATGAGTAAATTATATCATGCAAATTGTCATCATGAGGCAGGACGGTCCAAAAGGTAAGGTCAACAATACAATAGTCTTATGCCTCCAAAGGTTATAAATCATTCACTTAATATTTGTTTTTCTATCGTCTAATGTTATTAGTCTCATGTTCTACTATTTTCAAAATTAAAATTAATATTGTCTAAATAAAGATTGAATTAATTGATTTTATTATCAATTAATAAAGAAATATTAAACGAAATTAACATTATAACGTAATTAATGATTTTATATTCTAAGAAAATTTTGAAAAAAATTATTTTAAGAAAATTATTTATTTTACTAAAATATAATTATGATCATAGAAGACATCATCGTAAATTTGTCTTAGGCTTCTATATTTGTTGTGCTGACCCATACTATTAGGGTGCTTGTGACAAAATGGGTAATATTTATGTTTGTATGTCAGAAATGCTCAAGACCATACTAGAAAACAAACCAACTTGGATCCATTCAAGGGACAAACATGAAAGGCTTCCTCTTCATTACGCATCACCTATAGGTTACCTTGAAGGTGTTGAATTATTACTTGACGAATGCAAATGCTGCACAATTCAAAGGGACAAATATGGTTATTTCCCAATTCATCTAGCATCATATGGTGGCCATGTGGAAGTAGTGAAGAAGTTATTAGAATATTGTCCTGATCCCACAGAAATGCTTGACGCTTCTCATGAACGTAATATTCTTCACATTGCATCAAACTATGGAAAACATGGGGTGGTGCAATTCATTCTACAAAGTCAAAGTAGTGAACATTACAAGATGATAAATCAGAAAGATAGGAATGGAGACACCCCTTTGCATTTGGCTGCAAGATCATGTCATCCCACAACTGTGTACTACTTAGTTAACCAAAGCAAAGAAAGAGTGAAACTTGATTTGGTTAACCAAAACAATGAAACAGCTCTTGACATTGTTACTGCACCTTTCGAGGTTGATAAATCATCTTTGAGACAGGCAAGTAGTTCTATTCTCTCATAAACTTGCAAATTTATAACTAAGTTTACTGTATAGTTTTTTCTTATATATAAGTAAATCTAAATCAATGGTTAAACATCAATTTATTAATAGTGTATGCATAAGAATACATTCTTGATTAAAAAAAAAATTAATTTTAGGGAGATGTTATGACCTCTAAAGTGTTCTTCAACGGTTGTTAA
Protein sequence

>MS.gene057886.t1

MPHPEGKLFFAEPLQDEAVSRNVSSEKEPELEIEPSEVIEVIPKCCQFDNQINLLNEAYQLVYGVSTNLLDFSNLTKIETPMENTVLHIAAWYGNNDIVNLVIQHAPKLLFKFNKNYDSALHVAARGGHIPTVKKLLASYTNIGQHDIKREWLEYTKNNDDPEEYDEESNMEDLLKFVKEKNVQGNTMLREAMLSDKSNMSRDMIFIVCELYKTEDLSGYSLANSCYEFALDIINHAKKSVLYLAVENEDKDAVKLILKNSPKNDAKPKGLSPVVAAIMKQNQEMLKTILENKPTWIHSRDKHERLPLHYASPIGYLEGVELLLDECKCCTIQRDKYGYFPIHLASYGGHVEVVKKLLEYCPDPTEMLDASHERNILHIASNYGKHGVVQFILQSQSSEHYKMINQKDRNGDTPLHLAARSCHPTTVYYLVNQSKERVKLDLVNQNNETALDIVTAPFEGDVMTSKVFFNGC