AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070505

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070505.t1 MTR_5g085480 65.353 736 192 9 28 757 7 685 0.0 867
MS.gene070505.t1 MTR_5g085810 57.143 777 236 10 12 761 27 733 0.0 805
MS.gene070505.t1 MTR_5g085830 59.239 736 222 11 28 761 46 705 0.0 786
MS.gene070505.t1 MTR_5g085480 63.103 580 154 7 183 757 1 525 0.0 649
MS.gene070505.t1 MTR_2g033050 69.575 424 111 7 70 490 1 409 0.0 580
MS.gene070505.t1 MTR_5g085500 55.945 513 122 11 297 761 2 458 1.41e-175 512
MS.gene070505.t1 MTR_5g085490 72.302 278 72 3 6 281 18 292 4.65e-136 404
MS.gene070505.t1 MTR_5g085520 76.768 198 45 1 77 273 1 198 1.52e-99 306
MS.gene070505.t1 MTR_2g033045 74.375 160 41 0 603 762 60 219 3.83e-71 235
MS.gene070505.t1 MTR_5g085440 62.944 197 57 3 567 762 173 354 7.46e-67 226
MS.gene070505.t1 MTR_5g085440 72.973 148 38 2 6 153 14 159 9.95e-64 217
MS.gene070505.t1 MTR_5g085530 73.973 73 18 1 275 346 1 73 5.03e-32 119
MS.gene070505.t1 MTR_1g115575 23.327 523 306 14 231 746 128 562 1.92e-19 93.2
MS.gene070505.t1 MTR_2g101310 24.411 467 292 15 273 715 145 574 2.09e-16 84.0
MS.gene070505.t1 MTR_1g069110 31.551 187 113 3 282 466 30 203 1.14e-12 71.2
MS.gene070505.t1 MTR_1g032100 27.059 255 165 5 227 478 49 285 2.61e-12 70.5
MS.gene070505.t1 MTR_8g105260 21.778 450 230 17 303 713 101 467 4.65e-12 69.7
MS.gene070505.t1 MTR_5g085560 52.055 73 23 2 568 629 45 116 1.59e-11 62.8
MS.gene070505.t1 MTR_2g101320 23.810 399 261 10 273 657 145 514 1.75e-11 68.2
MS.gene070505.t1 MTR_2g096190 25.000 364 210 14 79 418 28 352 3.55e-11 67.0
MS.gene070505.t1 MTR_2g101310 26.761 213 129 6 273 481 145 334 4.00e-11 66.6
MS.gene070505.t1 MTR_7g021090 26.255 259 165 10 237 488 20 259 6.76e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070505.t1 AT4G03460 31.422 436 212 13 79 506 81 437 3.75e-37 148
MS.gene070505.t1 AT4G03460 39.375 160 91 1 603 756 458 617 5.78e-18 89.0
MS.gene070505.t1 AT4G03500 29.630 459 240 13 57 510 76 456 5.82e-37 148
MS.gene070505.t1 AT4G03500 37.952 166 96 2 603 762 474 638 6.52e-22 101
MS.gene070505.t1 AT4G03500 29.630 459 240 13 57 510 53 433 5.82e-37 147
MS.gene070505.t1 AT4G03500 37.952 166 96 2 603 762 451 615 5.29e-22 101
MS.gene070505.t1 AT4G03500 29.630 459 240 13 57 510 53 433 5.82e-37 147
MS.gene070505.t1 AT4G03500 37.952 166 96 2 603 762 451 615 5.29e-22 101
MS.gene070505.t1 AT4G03460 30.608 477 239 16 43 506 8 405 3.75e-35 142
MS.gene070505.t1 AT4G03460 39.375 160 91 1 603 756 426 585 6.82e-18 88.6
MS.gene070505.t1 AT1G03670 28.440 436 219 14 79 506 73 423 5.99e-34 138
MS.gene070505.t1 AT1G03670 34.286 175 105 4 596 762 438 610 1.47e-19 94.0
MS.gene070505.t1 AT4G03480 28.378 444 255 16 52 461 126 540 1.56e-30 128
MS.gene070505.t1 AT4G03440 27.815 453 250 15 74 483 114 532 1.45e-29 125
MS.gene070505.t1 AT4G03440 31.429 140 89 2 598 730 545 684 7.78e-13 72.8
MS.gene070505.t1 AT4G03440 27.815 453 250 15 74 483 107 525 1.46e-29 125
MS.gene070505.t1 AT4G03440 31.429 140 89 2 598 730 538 677 8.35e-13 72.4
MS.gene070505.t1 AT4G03440 27.815 453 250 15 74 483 107 525 1.46e-29 125
MS.gene070505.t1 AT4G03440 31.429 140 89 2 598 730 538 677 8.35e-13 72.4
MS.gene070505.t1 AT4G03480 28.222 450 245 17 52 461 126 537 3.09e-29 124
MS.gene070505.t1 AT4G05040 28.261 414 223 13 70 466 100 456 3.57e-27 117
MS.gene070505.t1 AT4G05040 28.261 414 223 13 70 466 100 456 3.57e-27 117
MS.gene070505.t1 AT4G05040 28.261 414 223 13 70 466 100 456 3.57e-27 117
MS.gene070505.t1 AT4G05040 28.261 414 223 13 70 466 100 456 3.57e-27 117
MS.gene070505.t1 AT4G05040 28.261 414 223 13 70 466 100 456 3.57e-27 117
MS.gene070505.t1 AT4G05040 28.261 414 223 13 70 466 100 456 3.57e-27 117
MS.gene070505.t1 AT4G05040 28.261 414 223 13 70 466 100 456 3.57e-27 117
MS.gene070505.t1 AT4G03470 28.715 397 222 11 70 463 111 449 1.79e-25 112
MS.gene070505.t1 AT4G03450 27.907 387 221 12 82 463 75 408 2.62e-25 112
MS.gene070505.t1 AT4G03450 22.862 538 325 16 231 712 68 571 7.99e-17 85.1
MS.gene070505.t1 AT4G03450 27.907 387 221 12 82 463 75 408 6.41e-25 110
MS.gene070505.t1 AT4G03450 22.774 584 359 17 231 756 68 617 3.12e-17 86.7
MS.gene070505.t1 AT4G03490 25.361 485 250 16 75 532 23 422 1.78e-23 106
MS.gene070505.t1 AT4G03490 31.707 164 106 2 603 760 429 592 8.50e-16 82.0
MS.gene070505.t1 AT4G03490 25.361 485 250 16 75 532 33 432 2.16e-23 105
MS.gene070505.t1 AT4G03490 31.707 164 106 2 603 760 439 602 8.90e-16 82.0
MS.gene070505.t1 AT4G03490 25.364 481 247 16 79 532 37 432 2.33e-23 106
MS.gene070505.t1 AT4G03490 33.333 144 86 4 603 740 439 578 3.17e-13 73.9
MS.gene070505.t1 AT4G14400 28.148 405 233 13 68 463 22 377 9.98e-23 103
MS.gene070505.t1 AT4G14400 28.148 405 233 13 68 463 22 377 9.98e-23 103
MS.gene070505.t1 AT4G14400 28.148 405 233 13 68 463 88 443 1.04e-22 103
MS.gene070505.t1 AT4G03490 26.042 384 202 11 75 431 23 351 1.37e-20 97.4
MS.gene070505.t1 AT4G03490 33.333 144 86 4 603 740 391 530 3.78e-13 73.6
MS.gene070505.t1 AT4G03490 26.042 384 202 11 75 431 13 341 1.76e-20 97.1
MS.gene070505.t1 AT4G03490 33.333 144 86 4 603 740 381 520 2.27e-13 74.3
MS.gene070505.t1 AT4G14390 26.276 392 238 13 88 460 89 448 3.03e-18 89.7
MS.gene070505.t1 AT4G14390 21.461 671 358 18 69 723 104 621 3.20e-16 83.6
MS.gene070505.t1 AT4G14390 26.276 392 238 13 88 460 108 467 3.05e-18 89.7
MS.gene070505.t1 AT4G14390 21.461 671 358 18 69 723 123 640 3.78e-16 83.2
MS.gene070505.t1 AT4G14390 21.726 672 356 18 69 723 123 641 6.43e-18 89.0
MS.gene070505.t1 AT4G14390 26.209 393 238 14 88 460 108 468 1.56e-17 87.8
MS.gene070505.t1 AT4G14390 26.463 393 238 14 88 461 89 449 7.07e-18 88.2
MS.gene070505.t1 AT5G02620 21.553 515 328 13 231 729 53 507 3.21e-12 70.5
MS.gene070505.t1 AT5G02620 21.553 515 328 13 231 729 53 507 3.21e-12 70.5
MS.gene070505.t1 AT5G02620 25.751 233 152 4 231 460 53 267 1.54e-11 68.2
MS.gene070505.t1 AT5G02620 25.751 233 152 4 231 460 53 267 1.54e-11 68.2
MS.gene070505.t1 AT5G02620 25.751 233 152 4 231 460 53 267 1.54e-11 68.2
MS.gene070505.t1 AT1G07710 27.039 233 147 5 231 459 63 276 3.71e-11 67.0
MS.gene070505.t1 AT1G07710 27.039 233 147 5 231 459 63 276 3.71e-11 67.0

Find 146 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTATTATCCTCCTCTTT+TGG 0.153837 5.4:+67698981 MS.gene070505:CDS
AGCCAACTTGGATCCATTTA+TGG 0.181919 5.4:+67697479 MS.gene070505:CDS
CTATTACCCCTTTCGTGTTC+TGG 0.206277 5.4:+67699249 MS.gene070505:CDS
TCTTCATCAGTGTATTTAAT+AGG 0.210812 5.4:-67698650 None:intergenic
TTATCAAATTTGCAACATTT+TGG 0.226185 5.4:-67695991 None:intergenic
CTCAATTTCTGTTAAATTAG+AGG 0.236158 5.4:-67696079 None:intergenic
CTGCAACGTGAAGAATATTT+TGG 0.243900 5.4:-67697720 None:intergenic
GTTAGAATACACTAACAATT+TGG 0.259464 5.4:+67696320 MS.gene070505:CDS
CCACTTCATCTAGCATCTTA+TGG 0.271180 5.4:+67697622 MS.gene070505:CDS
TTTCATGTGTGTCCCATAAA+TGG 0.276829 5.4:-67697492 None:intergenic
CTTCATCATTGCCTTCAATA+TGG 0.287756 5.4:-67698547 None:intergenic
CTCATAAATTTATAAGTTAC+AGG 0.289049 5.4:+67698067 MS.gene070505:CDS
CAAGAGCCGTTTCATTGTTC+TGG 0.291957 5.4:-67697966 None:intergenic
AAAATCGTAATGCCGCTTCT+TGG 0.312319 5.4:+67699043 MS.gene070505:CDS
TTAACTTTGAACAAAAGTTT+TGG 0.312467 5.4:-67696183 None:intergenic
GTCACTTGGTTTGGATGGTT+TGG 0.316130 5.4:-67698573 None:intergenic
AAAGGATTGAACCAAATAAA+AGG 0.317191 5.4:+67698696 MS.gene070505:CDS
CTTCATCAGTGTATTTAATA+GGG 0.320579 5.4:-67698649 None:intergenic
CTTCACGTTGCAGCAAACTA+TGG 0.331993 5.4:+67697730 MS.gene070505:CDS
GAAGCATATCACTTGGTATA+TGG 0.333817 5.4:+67696033 MS.gene070505:CDS
TACCATTCCAAGCTGCAATA+TGG 0.334649 5.4:-67696125 None:intergenic
TTGGGATTCGTCACTTGGTT+TGG 0.344554 5.4:-67698582 None:intergenic
ACATAACCGAAGACCGGTTC+GGG 0.346866 5.4:+67696502 MS.gene070505:CDS
CAGAACACGAAAGGGGTAAT+AGG 0.353948 5.4:-67699248 None:intergenic
CTGTTATCAGTGAACTTACT+TGG 0.359026 5.4:+67699113 MS.gene070505:CDS
CTACGATGAAAAGTCAAATA+TGG 0.359377 5.4:+67696356 MS.gene070505:CDS
CTTGGAACATTGCATGATAT+AGG 0.360849 5.4:-67698916 None:intergenic
TTTGTTAAAATTTGTGAAGA+TGG 0.361852 5.4:+67696383 MS.gene070505:CDS
TGGAGACACCCCTTTGCATT+TGG 0.362507 5.4:+67697864 MS.gene070505:CDS
CCCGGCCGAGAATCATTGTT+GGG 0.373270 5.4:-67696651 None:intergenic
TTTGAATTCTTCAAGTTGTC+CGG 0.373422 5.4:-67698675 None:intergenic
ACATTGTTAATTCGCTTTAC+CGG 0.373479 5.4:+67697989 MS.gene070505:CDS
GAGGAGGATAATAGAACTAA+TGG 0.390704 5.4:-67698975 None:intergenic
GCCAACTTGGATCCATTTAT+GGG 0.401430 5.4:+67697480 MS.gene070505:CDS
ACAATGCAAATGGACAAATA+TGG 0.403639 5.4:+67697592 MS.gene070505:CDS
TACATAACCGAAGACCGGTT+CGG 0.405176 5.4:+67696501 MS.gene070505:CDS
CCCAACAATGATTCTCGGCC+GGG 0.408526 5.4:+67696651 MS.gene070505:CDS
ATTGAATCCTCAGAAATAGT+TGG 0.409164 5.4:+67695961 MS.gene070505:CDS
GGATGCAGTCAAACTAATCT+TGG 0.416238 5.4:+67696620 MS.gene070505:CDS
TTCATGATTGCTTCTACAAC+GGG 0.419527 5.4:-67696684 None:intergenic
AACACTTGGGATAACTGAAT+TGG 0.425325 5.4:+67699006 MS.gene070505:CDS
TGAATTCCCGAACCGGTCTT+CGG 0.426970 5.4:-67696508 None:intergenic
AATGTTTGCAGCATCTTACA+TGG 0.427993 5.4:+67698474 MS.gene070505:intron
GCAGCATGTTTAGCTGTTCC+AGG 0.429250 5.4:+67698869 MS.gene070505:CDS
TCAGATTGGGATTCGTCACT+TGG 0.432415 5.4:-67698587 None:intergenic
GGAGGTTCAACGAATTTGTT+GGG 0.432471 5.4:+67696054 MS.gene070505:CDS
TTGAATCCTCAGAAATAGTT+GGG 0.434107 5.4:+67695962 MS.gene070505:CDS
ATACTAGAAAGTCAAGTTCA+TGG 0.435339 5.4:+67697775 MS.gene070505:CDS
CCCCGGCCGAGAATCATTGT+TGG 0.436970 5.4:-67696652 None:intergenic
TGGAGGTTCAACGAATTTGT+TGG 0.437402 5.4:+67696053 MS.gene070505:CDS
AGATTTGGTACAGTGTTGTC+GGG 0.445921 5.4:-67698758 None:intergenic
GTAATAATTCAACACCTTCA+AGG 0.447605 5.4:-67697549 None:intergenic
TCAAGCATCTCTGTGGGATC+AGG 0.449609 5.4:-67697685 None:intergenic
CCTGTCTTTATATGTAGTCT+TGG 0.450243 5.4:-67698798 None:intergenic
CTTCATGATTGCTTCTACAA+CGG 0.453527 5.4:-67696685 None:intergenic
GAAGGTGTTGAATTATTACT+TGG 0.460344 5.4:+67697553 MS.gene070505:CDS
GCATCTGCAGGTTACCTTGA+AGG 0.461021 5.4:+67697535 MS.gene070505:CDS
TTACCTTGCTGTTGAAAATG+AGG 0.465911 5.4:+67696593 MS.gene070505:CDS
CCCCAACAATGATTCTCGGC+CGG 0.466170 5.4:+67696650 MS.gene070505:CDS
AAGATTTGGTACAGTGTTGT+CGG 0.468064 5.4:-67698759 None:intergenic
GACAACTTGAAGAATTCAAA+AGG 0.470885 5.4:+67698678 MS.gene070505:CDS
CCAACAATGATTCTCGGCCG+GGG 0.472150 5.4:+67696652 MS.gene070505:CDS
TACTAGACAACAAGCCAACT+TGG 0.472905 5.4:+67697467 MS.gene070505:CDS
ATCTTGCAGCCAAATGCAAA+GGG 0.474929 5.4:-67697873 None:intergenic
ATGTCGTGACATTAAGAGAA+TGG 0.478360 5.4:+67696446 MS.gene070505:CDS
GATTCGTCACTTGGTTTGGA+TGG 0.480274 5.4:-67698578 None:intergenic
CAACAATGATTCTCGGCCGG+GGG 0.481772 5.4:+67696653 MS.gene070505:CDS
TGATAAAGATAATGAAAACT+TGG 0.486802 5.4:-67698934 None:intergenic
GTGAAGATGGAGAATGTTCA+AGG 0.486988 5.4:+67696396 MS.gene070505:CDS
ATTGCCCCAACAATGATTCT+CGG 0.487086 5.4:+67696646 MS.gene070505:CDS
CAGAAAGATAGTCGAGTTCG+TGG 0.489239 5.4:+67697808 MS.gene070505:CDS
CAAATGTTGCACAATGCAAA+TGG 0.490770 5.4:+67697582 MS.gene070505:CDS
AGAGCAGCAATCCATATTGA+AGG 0.493839 5.4:+67698536 MS.gene070505:CDS
TTGACAATGAATTCCCGAAC+CGG 0.497156 5.4:-67696515 None:intergenic
TAAATCATCATCTTTAAGAC+AGG 0.499647 5.4:+67698017 MS.gene070505:CDS
CCAAGACTACATATAAAGAC+AGG 0.501187 5.4:+67698798 MS.gene070505:CDS
CTTTCTGATTTATCATCTGA+CGG 0.501563 5.4:-67697834 None:intergenic
AAGATGATGATTTATCAAAC+CGG 0.502824 5.4:-67698008 None:intergenic
CTTCATTACGCAGCATCTGC+AGG 0.510686 5.4:+67697523 MS.gene070505:CDS
TCCCAAGTGTTGCCCAAAAG+AGG 0.518657 5.4:-67698994 None:intergenic
AGTGATGCCAGAACACGAAA+GGG 0.523082 5.4:-67699256 None:intergenic
TATCAGTGAACTTACTTGGT+TGG 0.523227 5.4:+67699117 MS.gene070505:CDS
TGTCGTGACATTAAGAGAAT+GGG 0.526860 5.4:+67696447 MS.gene070505:CDS
GTGACGAATCCCAATCTGAC+GGG 0.526999 5.4:+67698591 MS.gene070505:CDS
ACATCTAGCAGCAACATGTA+AGG 0.527159 5.4:-67696223 None:intergenic
ATAAATCAGAAAGATAAGAA+TGG 0.529202 5.4:+67697844 MS.gene070505:CDS
ACTAAGTAGAACACAGTTGT+GGG 0.534295 5.4:-67697904 None:intergenic
TTAGTAACAGTAACATCCCT+TGG 0.534332 5.4:-67695916 None:intergenic
TATAAATTTATGAGTGTGTG+AGG 0.535669 5.4:-67698058 None:intergenic
CCATAAGATGCTAGATGAAG+TGG 0.539522 5.4:-67697622 None:intergenic
AACAGTAACATCCCTTGGTA+AGG 0.539827 5.4:-67695911 None:intergenic
GCAATCATGAAGCATAATCA+AGG 0.540828 5.4:+67696696 MS.gene070505:CDS
TTGAATTCTTCAAGTTGTCC+GGG 0.541542 5.4:-67698674 None:intergenic
TTCTAGTTGATGATGATGGA+AGG 0.542591 5.4:-67699207 None:intergenic
CTCCATATTGCAGCTTGGAA+TGG 0.551821 5.4:+67696123 MS.gene070505:CDS
GCTTAGTGAAGCATATCACT+TGG 0.554255 5.4:+67696026 MS.gene070505:CDS
ATAAATTTATGAGTGTGTGA+GGG 0.555112 5.4:-67698057 None:intergenic
AACTTTACATAACCGAAGAC+CGG 0.561024 5.4:+67696496 MS.gene070505:CDS
AACAATGATTCTCGGCCGGG+GGG 0.565266 5.4:+67696654 MS.gene070505:CDS
ACTTGCTAATAGCTTTCTAA+CGG 0.567233 5.4:-67696259 None:intergenic
TCCCATAAATGGATCCAAGT+TGG 0.567330 5.4:-67697481 None:intergenic
GGTTAACCAGAACAATGAAA+CGG 0.567491 5.4:+67697960 MS.gene070505:CDS
TGGACTGCACTGAAATCTGC+TGG 0.569090 5.4:+67698494 MS.gene070505:CDS
GATCTTGCAGCCAAATGCAA+AGG 0.573294 5.4:-67697874 None:intergenic
AACTAAGTAGAACACAGTTG+TGG 0.574343 5.4:-67697905 None:intergenic
TACAAAATGAAGCCTTACCA+AGG 0.575582 5.4:+67695899 MS.gene070505:CDS
AAGTGATGCCAGAACACGAA+AGG 0.575786 5.4:-67699257 None:intergenic
TGACATTAAGAGAATGGGCG+GGG 0.576720 5.4:+67696452 MS.gene070505:CDS
GTAATAGGAAATGTATCGCA+TGG 0.576809 5.4:-67699233 None:intergenic
TCAACGGAGTGATATAAAAG+TGG 0.580462 5.4:+67696293 MS.gene070505:CDS
CAAGTTATGCTAATATTCAA+CGG 0.581179 5.4:+67696277 MS.gene070505:CDS
GCTGTTCCAGGTGAATCAGA+AGG 0.581471 5.4:+67698881 MS.gene070505:CDS
TTGATGATGATGGAAGGAAA+AGG 0.582418 5.4:-67699201 None:intergenic
AAGAAACCCAACTATTTCTG+AGG 0.582997 5.4:-67695968 None:intergenic
CACTATAAGTGTTGAAACCA+AGG 0.585737 5.4:-67698834 None:intergenic
TTACATGTTGCTGCTAGATG+TGG 0.588475 5.4:+67696225 MS.gene070505:CDS
TAAATTTATGAGTGTGTGAG+GGG 0.595862 5.4:-67698056 None:intergenic
GTGACATTAAGAGAATGGGC+GGG 0.598103 5.4:+67696451 MS.gene070505:CDS
GCAGATGCTGCGTAATGAAG+TGG 0.601792 5.4:-67697520 None:intergenic
CAGGGTAGAGACACTCACCT+TGG 0.604258 5.4:+67698817 MS.gene070505:CDS
ATTTATGGGACACACATGAA+AGG 0.604599 5.4:+67697494 MS.gene070505:CDS
AAATACACTGATGAAGAACC+CGG 0.608226 5.4:+67698656 MS.gene070505:CDS
CAGTGCTCCATATTGCAGCT+TGG 0.610920 5.4:+67696118 MS.gene070505:CDS
GAAAGGTCAAGCATCTCTGT+GGG 0.613946 5.4:-67697691 None:intergenic
ACAATGATTCTCGGCCGGGG+GGG 0.615973 5.4:+67696655 MS.gene070505:CDS
ATGAGAGCGATTCCAAGAAG+CGG 0.617860 5.4:-67699055 None:intergenic
GTTCTGGCATCACTTGCAGA+AGG 0.621716 5.4:+67699265 MS.gene070505:CDS
CGATGAAAAGTCAAATATGG+AGG 0.630705 5.4:+67696359 MS.gene070505:CDS
TTATGGTGGCCATGTGAAAG+TGG 0.632273 5.4:+67697639 MS.gene070505:CDS
AGAAAGGTCAAGCATCTCTG+TGG 0.634118 5.4:-67697692 None:intergenic
GCATATCACTTGGTATATGG+AGG 0.638334 5.4:+67696036 MS.gene070505:CDS
TGCTGTTGAAAATGAGGACA+AGG 0.639852 5.4:+67696599 MS.gene070505:CDS
CATAAGATGCTAGATGAAGT+GGG 0.641628 5.4:-67697621 None:intergenic
CCGGCCGAGAATCATTGTTG+GGG 0.641969 5.4:-67696650 None:intergenic
CAAGTGTTGCCCAAAAGAGG+AGG 0.648243 5.4:-67698991 None:intergenic
CGTGACATTAAGAGAATGGG+CGG 0.650249 5.4:+67696450 MS.gene070505:CDS
AGTGACGAATCCCAATCTGA+CGG 0.652754 5.4:+67698590 MS.gene070505:CDS
ACAAAATGAAGCCTTACCAA+GGG 0.657332 5.4:+67695900 MS.gene070505:CDS
CTTCATCTAGCATCTTATGG+TGG 0.665167 5.4:+67697625 MS.gene070505:CDS
GATTTGGTACAGTGTTGTCG+GGG 0.670917 5.4:-67698757 None:intergenic
GGAGTGATATAAAAGTGGCA+TGG 0.672750 5.4:+67696298 MS.gene070505:CDS
AGGCAACACTATGTTACATG+AGG 0.678880 5.4:+67696416 MS.gene070505:CDS
TGACGAATCCCAATCTGACG+GGG 0.679122 5.4:+67698592 MS.gene070505:CDS
GTGATGCCAGAACACGAAAG+GGG 0.680402 5.4:-67699255 None:intergenic
CAAGACTACATATAAAGACA+GGG 0.682588 5.4:+67698799 MS.gene070505:CDS
TCTTGCAGCCAAATGCAAAG+GGG 0.746892 5.4:-67697872 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAATGTATAAATAAA+GGG - chr5.4:67698364-67698383 None:intergenic 10.0%
!! TAAGAAAAATGTATAAATAA+AGG - chr5.4:67698365-67698384 None:intergenic 10.0%
!! TCAAAATAACATTTATATTA+GGG - chr5.4:67697104-67697123 None:intergenic 10.0%
!! TTCAAAATAACATTTATATT+AGG - chr5.4:67697105-67697124 None:intergenic 10.0%
!!! TTTTTTTTTTTTTTTTGTTG+AGG + chr5.4:67697291-67697310 MS.gene070505:intron 10.0%
!! AAAATGGAATATATTGTATT+GGG - chr5.4:67698340-67698359 None:intergenic 15.0%
!! AGAAAAATGTATAAATAAAG+GGG - chr5.4:67698363-67698382 None:intergenic 15.0%
!! ATTTAGTGTTATATTGTATT+GGG - chr5.4:67698299-67698318 None:intergenic 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - chr5.4:67698300-67698319 None:intergenic 15.0%
!!! ACTTTCAAAATTTACAATTT+TGG + chr5.4:67696768-67696787 MS.gene070505:intron 15.0%
!! ATAAATCAGAAAGATAAGAA+TGG + chr5.4:67697844-67697863 MS.gene070505:CDS 20.0%
!! CTCATAAATTTATAAGTTAC+AGG + chr5.4:67698067-67698086 MS.gene070505:CDS 20.0%
!! GAAAATGGAATATATTGTAT+TGG - chr5.4:67698341-67698360 None:intergenic 20.0%
!! TGATAAAGATAATGAAAACT+TGG - chr5.4:67698937-67698956 None:intergenic 20.0%
!! TTTGTTAAAATTTGTGAAGA+TGG + chr5.4:67696383-67696402 MS.gene070505:CDS 20.0%
!!! GAATGAATAAAGATTTTATG+AGG + chr5.4:67697362-67697381 MS.gene070505:intron 20.0%
!!! TTAACTTTGAACAAAAGTTT+TGG - chr5.4:67696186-67696205 None:intergenic 20.0%
!!! TTATCAAATTTGCAACATTT+TGG - chr5.4:67695994-67696013 None:intergenic 20.0%
! AAAAGCTAACAAAAAAGAAG+AGG - chr5.4:67697271-67697290 None:intergenic 25.0%
! AAAGCTAACAAAAAAGAAGA+GGG - chr5.4:67697270-67697289 None:intergenic 25.0%
! AAAGGATTGAACCAAATAAA+AGG + chr5.4:67698696-67698715 MS.gene070505:CDS 25.0%
! AAGATGATGATTTATCAAAC+CGG - chr5.4:67698011-67698030 None:intergenic 25.0%
! AATCATAACCAGAAAAACAT+TGG - chr5.4:67699143-67699162 None:intergenic 25.0%
! ATAAATTTATGAGTGTGTGA+GGG - chr5.4:67698060-67698079 None:intergenic 25.0%
! CAAGTTATGCTAATATTCAA+CGG + chr5.4:67696277-67696296 MS.gene070505:CDS 25.0%
! CTCAATTTCTGTTAAATTAG+AGG - chr5.4:67696082-67696101 None:intergenic 25.0%
! CTTACTTATAATACTTCTTC+CGG + chr5.4:67696953-67696972 MS.gene070505:intron 25.0%
! CTTCATCAGTGTATTTAATA+GGG - chr5.4:67698652-67698671 None:intergenic 25.0%
! CTTTATTCATTCTGATCAAT+TGG - chr5.4:67697354-67697373 None:intergenic 25.0%
! GTTAGAATACACTAACAATT+TGG + chr5.4:67696320-67696339 MS.gene070505:CDS 25.0%
! TATAAATTTATGAGTGTGTG+AGG - chr5.4:67698061-67698080 None:intergenic 25.0%
! TCAAAACATTGTTGAATTCA+AGG - chr5.4:67696883-67696902 None:intergenic 25.0%
! TCTTCATCAGTGTATTTAAT+AGG - chr5.4:67698653-67698672 None:intergenic 25.0%
! TGTATAAATAAAGGGGAAAA+TGG - chr5.4:67698356-67698375 None:intergenic 25.0%
! TTGACAACGATTATAAACTT+CGG + chr5.4:67698114-67698133 MS.gene070505:intron 25.0%
!! AATTTGTTTGTAATAGCAAC+CGG - chr5.4:67696975-67696994 None:intergenic 25.0%
!! AGTTTATTGATTAACCGATA+TGG + chr5.4:67697006-67697025 MS.gene070505:intron 25.0%
!! TATGATTGTGATTTTTGTTG+TGG + chr5.4:67699156-67699175 MS.gene070505:CDS 25.0%
!! TTGAAATGACTCAGTTATTT+TGG - chr5.4:67698253-67698272 None:intergenic 25.0%
!!! GACACTTTTTTTTAGAAAGT+GGG - chr5.4:67698230-67698249 None:intergenic 25.0%
!!! GGAAGAAGTATTATAAGTAA+GGG - chr5.4:67696954-67696973 None:intergenic 25.0%
!!! GTTCAAAATGTTTTAAGTCA+GGG - chr5.4:67697130-67697149 None:intergenic 25.0%
!!! TAAATCATCATCTTTAAGAC+AGG + chr5.4:67698017-67698036 MS.gene070505:CDS 25.0%
!!! TCGTTACTTTTCCTTTTATT+TGG - chr5.4:67698710-67698729 None:intergenic 25.0%
!!! TGAAATGACTCAGTTATTTT+GGG - chr5.4:67698252-67698271 None:intergenic 25.0%
ACAATGCAAATGGACAAATA+TGG + chr5.4:67697592-67697611 MS.gene070505:CDS 30.0%
ACATTGTTAATTCGCTTTAC+CGG + chr5.4:67697989-67698008 MS.gene070505:CDS 30.0%
ATACTAGAAAGTCAAGTTCA+TGG + chr5.4:67697775-67697794 MS.gene070505:CDS 30.0%
ATTGAATCCTCAGAAATAGT+TGG + chr5.4:67695961-67695980 MS.gene070505:CDS 30.0%
CAAGACTACATATAAAGACA+GGG + chr5.4:67698799-67698818 MS.gene070505:CDS 30.0%
CTACGATGAAAAGTCAAATA+TGG + chr5.4:67696356-67696375 MS.gene070505:CDS 30.0%
CTTTCTGATTTATCATCTGA+CGG - chr5.4:67697837-67697856 None:intergenic 30.0%
GACAACTTGAAGAATTCAAA+AGG + chr5.4:67698678-67698697 MS.gene070505:CDS 30.0%
GTAATAATTCAACACCTTCA+AGG - chr5.4:67697552-67697571 None:intergenic 30.0%
TAAATTTATGAGTGTGTGAG+GGG - chr5.4:67698059-67698078 None:intergenic 30.0%
TATAAGTAAGGGAAATGCTA+AGG - chr5.4:67696943-67696962 None:intergenic 30.0%
TTGAATCCTCAGAAATAGTT+GGG + chr5.4:67695962-67695981 MS.gene070505:CDS 30.0%
TTTCCTTAACCATTGTCTTA+AGG - chr5.4:67696842-67696861 None:intergenic 30.0%
! ACTTGCTAATAGCTTTCTAA+CGG - chr5.4:67696262-67696281 None:intergenic 30.0%
! ACTTTTCATCGTAGTCTTTT+GGG - chr5.4:67696350-67696369 None:intergenic 30.0%
! AGTGCAGTTTTTATAATTCC+AGG - chr5.4:67697077-67697096 None:intergenic 30.0%
! GAAAGAGTTTGTTTGCATTT+GGG - chr5.4:67697159-67697178 None:intergenic 30.0%
! GTGCAGTTTTTATAATTCCA+GGG - chr5.4:67697076-67697095 None:intergenic 30.0%
! TGAAAGAGTTTGTTTGCATT+TGG - chr5.4:67697160-67697179 None:intergenic 30.0%
! TTCTATTATCCTCCTCTTTT+GGG + chr5.4:67698982-67699001 MS.gene070505:CDS 30.0%
! TTTTGTACGGGAATAAAAAC+AGG - chr5.4:67695886-67695905 None:intergenic 30.0%
!! CGGAAGAAGTATTATAAGTA+AGG - chr5.4:67696955-67696974 None:intergenic 30.0%
!! GAAGGTGTTGAATTATTACT+TGG + chr5.4:67697553-67697572 MS.gene070505:CDS 30.0%
!! GGTTCAAAATGTTTTAAGTC+AGG - chr5.4:67697131-67697150 None:intergenic 30.0%
!! TTTGAATTCTTCAAGTTGTC+CGG - chr5.4:67698678-67698697 None:intergenic 30.0%
!!! CACTGTACCAAATCTTTTTT+TGG + chr5.4:67698766-67698785 MS.gene070505:CDS 30.0%
!!! GAATATTTTGGTCAAGAGAA+AGG - chr5.4:67697711-67697730 None:intergenic 30.0%
!!! GATTTTCTAGTTGATGATGA+TGG - chr5.4:67699214-67699233 None:intergenic 30.0%
!!! GGACACTTTTTTTTAGAAAG+TGG - chr5.4:67698231-67698250 None:intergenic 30.0%
!!! TTCACTATTTTTGGCTTTCA+TGG + chr5.4:67699078-67699097 MS.gene070505:CDS 30.0%
AAATACACTGATGAAGAACC+CGG + chr5.4:67698656-67698675 MS.gene070505:CDS 35.0%
AACACTTGGGATAACTGAAT+TGG + chr5.4:67699006-67699025 MS.gene070505:CDS 35.0%
AACTAAGTAGAACACAGTTG+TGG - chr5.4:67697908-67697927 None:intergenic 35.0%
AACTTTACATAACCGAAGAC+CGG + chr5.4:67696496-67696515 MS.gene070505:CDS 35.0%
AATGTTTGCAGCATCTTACA+TGG + chr5.4:67698474-67698493 MS.gene070505:intron 35.0%
ACAAAATGAAGCCTTACCAA+GGG + chr5.4:67695900-67695919 MS.gene070505:CDS 35.0%
ACTAAGTAGAACACAGTTGT+GGG - chr5.4:67697907-67697926 None:intergenic 35.0%
AGGCAGATTGCAATTATTAG+GGG + chr5.4:67697382-67697401 MS.gene070505:intron 35.0%
ATGTCGTGACATTAAGAGAA+TGG + chr5.4:67696446-67696465 MS.gene070505:CDS 35.0%
ATTTATGGGACACACATGAA+AGG + chr5.4:67697494-67697513 MS.gene070505:CDS 35.0%
CAAATGTTGCACAATGCAAA+TGG + chr5.4:67697582-67697601 MS.gene070505:CDS 35.0%
CATAAGATGCTAGATGAAGT+GGG - chr5.4:67697624-67697643 None:intergenic 35.0%
CCAAGACTACATATAAAGAC+AGG + chr5.4:67698798-67698817 MS.gene070505:CDS 35.0%
CCTGTCTTTATATGTAGTCT+TGG - chr5.4:67698801-67698820 None:intergenic 35.0%
CCTTTCGCCAAAAAAAGATT+TGG - chr5.4:67698776-67698795 None:intergenic 35.0%
CGATGAAAAGTCAAATATGG+AGG + chr5.4:67696359-67696378 MS.gene070505:CDS 35.0%
CTGTTATCAGTGAACTTACT+TGG + chr5.4:67699113-67699132 MS.gene070505:CDS 35.0%
CTTCATCATTGCCTTCAATA+TGG - chr5.4:67698550-67698569 None:intergenic 35.0%
CTTCATGATTGCTTCTACAA+CGG - chr5.4:67696688-67696707 None:intergenic 35.0%
CTTGGAACATTGCATGATAT+AGG - chr5.4:67698919-67698938 None:intergenic 35.0%
GAAGCATATCACTTGGTATA+TGG + chr5.4:67696033-67696052 MS.gene070505:CDS 35.0%
GAGGAGGATAATAGAACTAA+TGG - chr5.4:67698978-67698997 None:intergenic 35.0%
GAGGCAGATTGCAATTATTA+GGG + chr5.4:67697381-67697400 MS.gene070505:intron 35.0%
GCAATCATGAAGCATAATCA+AGG + chr5.4:67696696-67696715 MS.gene070505:CDS 35.0%
GGTTAACCAGAACAATGAAA+CGG + chr5.4:67697960-67697979 MS.gene070505:CDS 35.0%
GTAATAGGAAATGTATCGCA+TGG - chr5.4:67699236-67699255 None:intergenic 35.0%
TACAAAATGAAGCCTTACCA+AGG + chr5.4:67695899-67695918 MS.gene070505:CDS 35.0%
TATCAGTGAACTTACTTGGT+TGG + chr5.4:67699117-67699136 MS.gene070505:CDS 35.0%
TCAACGGAGTGATATAAAAG+TGG + chr5.4:67696293-67696312 MS.gene070505:CDS 35.0%
TGAGGCAGATTGCAATTATT+AGG + chr5.4:67697380-67697399 MS.gene070505:intron 35.0%
TGTCCTTAAGACAATGGTTA+AGG + chr5.4:67696836-67696855 MS.gene070505:intron 35.0%
TGTCGTGACATTAAGAGAAT+GGG + chr5.4:67696447-67696466 MS.gene070505:CDS 35.0%
TTAGTAACAGTAACATCCCT+TGG - chr5.4:67695919-67695938 None:intergenic 35.0%
TTCATGATTGCTTCTACAAC+GGG - chr5.4:67696687-67696706 None:intergenic 35.0%
TTGATTAACCGATATGGACT+CGG + chr5.4:67697012-67697031 MS.gene070505:intron 35.0%
TTTCATGTGTGTCCCATAAA+TGG - chr5.4:67697495-67697514 None:intergenic 35.0%
! AAGATTTGGTACAGTGTTGT+CGG - chr5.4:67698762-67698781 None:intergenic 35.0%
! ACTTTTCTACCACTTTCACA+TGG - chr5.4:67697651-67697670 None:intergenic 35.0%
! CGAAAAAGTGCATCTTGATA+TGG + chr5.4:67697939-67697958 MS.gene070505:CDS 35.0%
! CGCTCTCATTTCACTATTTT+TGG + chr5.4:67699069-67699088 MS.gene070505:CDS 35.0%
! CTGCAACGTGAAGAATATTT+TGG - chr5.4:67697723-67697742 None:intergenic 35.0%
! GACTTTTCATCGTAGTCTTT+TGG - chr5.4:67696351-67696370 None:intergenic 35.0%
! GTTCTATTATCCTCCTCTTT+TGG + chr5.4:67698981-67699000 MS.gene070505:CDS 35.0%
! TGGTAAGGCTTCATTTTGTA+CGG - chr5.4:67695899-67695918 None:intergenic 35.0%
!! AAGAAACCCAACTATTTCTG+AGG - chr5.4:67695971-67695990 None:intergenic 35.0%
!! CACTATAAGTGTTGAAACCA+AGG - chr5.4:67698837-67698856 None:intergenic 35.0%
!! GAGCACTGTATTTTTCATAG+GGG - chr5.4:67696106-67696125 None:intergenic 35.0%
!! GGACTTTTTCTTTACTCTGT+TGG - chr5.4:67698739-67698758 None:intergenic 35.0%
!! TTACCTTGCTGTTGAAAATG+AGG + chr5.4:67696593-67696612 MS.gene070505:CDS 35.0%
!! TTCTAGTTGATGATGATGGA+AGG - chr5.4:67699210-67699229 None:intergenic 35.0%
!! TTGAATTCTTCAAGTTGTCC+GGG - chr5.4:67698677-67698696 None:intergenic 35.0%
!! TTGATGATGATGGAAGGAAA+AGG - chr5.4:67699204-67699223 None:intergenic 35.0%
!!! CCAAATCTTTTTTTGGCGAA+AGG + chr5.4:67698773-67698792 MS.gene070505:CDS 35.0%
!!! GATGCACTTTTTCGAGATTT+TGG - chr5.4:67697933-67697952 None:intergenic 35.0%
!!! GGAGCACTGTATTTTTCATA+GGG - chr5.4:67696107-67696126 None:intergenic 35.0%
!!! TGGAGCACTGTATTTTTCAT+AGG - chr5.4:67696108-67696127 None:intergenic 35.0%
AACAGTAACATCCCTTGGTA+AGG - chr5.4:67695914-67695933 None:intergenic 40.0%
AACCAGTGTCCTTAAGACAA+TGG + chr5.4:67696830-67696849 MS.gene070505:intron 40.0%
AACCATTGTCTTAAGGACAC+TGG - chr5.4:67696835-67696854 None:intergenic 40.0%
AATCTGTGTGAAATGAGCCA+AGG + chr5.4:67698384-67698403 MS.gene070505:intron 40.0%
ACATCTAGCAGCAACATGTA+AGG - chr5.4:67696226-67696245 None:intergenic 40.0%
AGAAAGTGGGTCAGATATTC+CGG - chr5.4:67698217-67698236 None:intergenic 40.0%
AGAGCAGCAATCCATATTGA+AGG + chr5.4:67698536-67698555 MS.gene070505:CDS 40.0%
AGCCAACTTGGATCCATTTA+TGG + chr5.4:67697479-67697498 MS.gene070505:CDS 40.0%
AGGCAACACTATGTTACATG+AGG + chr5.4:67696416-67696435 MS.gene070505:CDS 40.0%
ATCTGTGTGAAATGAGCCAA+GGG + chr5.4:67698385-67698404 MS.gene070505:intron 40.0%
ATCTTGCAGCCAAATGCAAA+GGG - chr5.4:67697876-67697895 None:intergenic 40.0%
ATTGCCCCAACAATGATTCT+CGG + chr5.4:67696646-67696665 MS.gene070505:CDS 40.0%
CCACTTCATCTAGCATCTTA+TGG + chr5.4:67697622-67697641 MS.gene070505:CDS 40.0%
CCATAAGATGCTAGATGAAG+TGG - chr5.4:67697625-67697644 None:intergenic 40.0%
CTTCATCTAGCATCTTATGG+TGG + chr5.4:67697625-67697644 MS.gene070505:CDS 40.0%
GCAGATTGCAATTATTAGGG+GGG + chr5.4:67697384-67697403 MS.gene070505:intron 40.0%
GCATATCACTTGGTATATGG+AGG + chr5.4:67696036-67696055 MS.gene070505:CDS 40.0%
GCCAACTTGGATCCATTTAT+GGG + chr5.4:67697480-67697499 MS.gene070505:CDS 40.0%
GCTTAGTGAAGCATATCACT+TGG + chr5.4:67696026-67696045 MS.gene070505:CDS 40.0%
GGAGTGATATAAAAGTGGCA+TGG + chr5.4:67696298-67696317 MS.gene070505:CDS 40.0%
GGATGCAGTCAAACTAATCT+TGG + chr5.4:67696620-67696639 MS.gene070505:CDS 40.0%
GGCAAACCACATTGTATTGT+GGG + chr5.4:67697036-67697055 MS.gene070505:intron 40.0%
GGCAGATTGCAATTATTAGG+GGG + chr5.4:67697383-67697402 MS.gene070505:intron 40.0%
GGTATACCCACAATACAATG+TGG - chr5.4:67697045-67697064 None:intergenic 40.0%
GTGAAGATGGAGAATGTTCA+AGG + chr5.4:67696396-67696415 MS.gene070505:CDS 40.0%
TACCATTCCAAGCTGCAATA+TGG - chr5.4:67696128-67696147 None:intergenic 40.0%
TACTAGACAACAAGCCAACT+TGG + chr5.4:67697467-67697486 MS.gene070505:CDS 40.0%
TCCCATAAATGGATCCAAGT+TGG - chr5.4:67697484-67697503 None:intergenic 40.0%
TGCTGTTGAAAATGAGGACA+AGG + chr5.4:67696599-67696618 MS.gene070505:CDS 40.0%
TGGAGGTTCAACGAATTTGT+TGG + chr5.4:67696053-67696072 MS.gene070505:CDS 40.0%
TGGCAAACCACATTGTATTG+TGG + chr5.4:67697035-67697054 MS.gene070505:intron 40.0%
TTACATGTTGCTGCTAGATG+TGG + chr5.4:67696225-67696244 MS.gene070505:CDS 40.0%
TTGACAATGAATTCCCGAAC+CGG - chr5.4:67696518-67696537 None:intergenic 40.0%
TTTGTTTGCATTTGGGCACT+CGG - chr5.4:67697152-67697171 None:intergenic 40.0%
! AAAATCGTAATGCCGCTTCT+TGG + chr5.4:67699043-67699062 MS.gene070505:CDS 40.0%
! AGATTTGGTACAGTGTTGTC+GGG - chr5.4:67698761-67698780 None:intergenic 40.0%
! GGAGGTTCAACGAATTTGTT+GGG + chr5.4:67696054-67696073 MS.gene070505:CDS 40.0%
! GGTAAGGCTTCATTTTGTAC+GGG - chr5.4:67695898-67695917 None:intergenic 40.0%
! TGTCCTCATTTTCAACAGCA+AGG - chr5.4:67696599-67696618 None:intergenic 40.0%
!! GCGGCACTGATTTCATTATT+TGG + chr5.4:67697406-67697425 MS.gene070505:intron 40.0%
!! TTGGTTGGCCAATGTTTTTC+TGG + chr5.4:67699132-67699151 MS.gene070505:CDS 40.0%
!!! CTATTTTTGGCTTTCATGGC+TGG + chr5.4:67699082-67699101 MS.gene070505:CDS 40.0%
AAGTGATGCCAGAACACGAA+AGG - chr5.4:67699260-67699279 None:intergenic 45.0%
AGAAAGGTCAAGCATCTCTG+TGG - chr5.4:67697695-67697714 None:intergenic 45.0%
AGCCAAGGGCTCAGATAAAT+TGG + chr5.4:67698399-67698418 MS.gene070505:intron 45.0%
AGGGCTCAGATAAATTGGGA+CGG + chr5.4:67698404-67698423 MS.gene070505:intron 45.0%
AGTGACGAATCCCAATCTGA+CGG + chr5.4:67698590-67698609 MS.gene070505:CDS 45.0%
AGTGATGCCAGAACACGAAA+GGG - chr5.4:67699259-67699278 None:intergenic 45.0%
ATGAGAGCGATTCCAAGAAG+CGG - chr5.4:67699058-67699077 None:intergenic 45.0%
ATTAACCGATATGGACTCGG+TGG + chr5.4:67697015-67697034 MS.gene070505:intron 45.0%
CAAGAGCCGTTTCATTGTTC+TGG - chr5.4:67697969-67697988 None:intergenic 45.0%
CACGAGTAAGAGAGAGAACT+CGG - chr5.4:67697199-67697218 None:intergenic 45.0%
CAGAAAGATAGTCGAGTTCG+TGG + chr5.4:67697808-67697827 MS.gene070505:CDS 45.0%
CAGAACACGAAAGGGGTAAT+AGG - chr5.4:67699251-67699270 None:intergenic 45.0%
CGTGACATTAAGAGAATGGG+CGG + chr5.4:67696450-67696469 MS.gene070505:CDS 45.0%
CTATTACCCCTTTCGTGTTC+TGG + chr5.4:67699249-67699268 MS.gene070505:CDS 45.0%
CTCAGATAAATTGGGACGGA+GGG + chr5.4:67698408-67698427 MS.gene070505:intron 45.0%
CTCCATATTGCAGCTTGGAA+TGG + chr5.4:67696123-67696142 MS.gene070505:CDS 45.0%
CTTCACGTTGCAGCAAACTA+TGG + chr5.4:67697730-67697749 MS.gene070505:CDS 45.0%
GAAAGGTCAAGCATCTCTGT+GGG - chr5.4:67697694-67697713 None:intergenic 45.0%
GAAAGTGGGTCAGATATTCC+GGG - chr5.4:67698216-67698235 None:intergenic 45.0%
GATCTTGCAGCCAAATGCAA+AGG - chr5.4:67697877-67697896 None:intergenic 45.0%
GATTGCAATTATTAGGGGGG+CGG + chr5.4:67697387-67697406 MS.gene070505:intron 45.0%
GCCAAGGGCTCAGATAAATT+GGG + chr5.4:67698400-67698419 MS.gene070505:intron 45.0%
GTGACATTAAGAGAATGGGC+GGG + chr5.4:67696451-67696470 MS.gene070505:CDS 45.0%
TACATAACCGAAGACCGGTT+CGG + chr5.4:67696501-67696520 MS.gene070505:CDS 45.0%
TATAATTCCAGGGTGTGCAG+CGG - chr5.4:67697066-67697085 None:intergenic 45.0%
TCAGATTGGGATTCGTCACT+TGG - chr5.4:67698590-67698609 None:intergenic 45.0%
TCCCAATTTATCTGAGCCCT+TGG - chr5.4:67698404-67698423 None:intergenic 45.0%
TCTTGCAGCCAAATGCAAAG+GGG - chr5.4:67697875-67697894 None:intergenic 45.0%
TGACATTAAGAGAATGGGCG+GGG + chr5.4:67696452-67696471 MS.gene070505:CDS 45.0%
TTATGGTGGCCATGTGAAAG+TGG + chr5.4:67697639-67697658 MS.gene070505:CDS 45.0%
TTGGGATTCGTCACTTGGTT+TGG - chr5.4:67698585-67698604 None:intergenic 45.0%
! GATTTGGTACAGTGTTGTCG+GGG - chr5.4:67698760-67698779 None:intergenic 45.0%
! GCTTTTCCTTCTGATTCACC+TGG - chr5.4:67698890-67698909 None:intergenic 45.0%
! TCCTCTTTTGGGCAACACTT+GGG + chr5.4:67698993-67699012 MS.gene070505:CDS 45.0%
!! AGAGAGAACTCGGTGCAAAA+AGG - chr5.4:67697189-67697208 None:intergenic 45.0%
!! GATTCGTCACTTGGTTTGGA+TGG - chr5.4:67698581-67698600 None:intergenic 45.0%
!! GTCACTTGGTTTGGATGGTT+TGG - chr5.4:67698576-67698595 None:intergenic 45.0%
!! TTGCTTTTCCCCGTCAGATT+GGG - chr5.4:67698603-67698622 None:intergenic 45.0%
!! TTTGCTTTTCCCCGTCAGAT+TGG - chr5.4:67698604-67698623 None:intergenic 45.0%
!!! TAATTTAATTTTTACTTATA+GGG + chr5.4:67696800-67696819 MS.gene070505:intron 5.0%
!!! TTAATTTAATTTTTACTTAT+AGG + chr5.4:67696799-67696818 MS.gene070505:intron 5.0%
CAGTGCTCCATATTGCAGCT+TGG + chr5.4:67696118-67696137 MS.gene070505:CDS 50.0%
CTTCATTACGCAGCATCTGC+AGG + chr5.4:67697523-67697542 MS.gene070505:CDS 50.0%
GCAGATGCTGCGTAATGAAG+TGG - chr5.4:67697523-67697542 None:intergenic 50.0%
GCAGCATGTTTAGCTGTTCC+AGG + chr5.4:67698869-67698888 MS.gene070505:CDS 50.0%
GCATCTGCAGGTTACCTTGA+AGG + chr5.4:67697535-67697554 MS.gene070505:CDS 50.0%
GCTCAGATAAATTGGGACGG+AGG + chr5.4:67698407-67698426 MS.gene070505:intron 50.0%
GCTGTTCCAGGTGAATCAGA+AGG + chr5.4:67698881-67698900 MS.gene070505:CDS 50.0%
GTGACGAATCCCAATCTGAC+GGG + chr5.4:67698591-67698610 MS.gene070505:CDS 50.0%
GTGATGCCAGAACACGAAAG+GGG - chr5.4:67699258-67699277 None:intergenic 50.0%
GTTCTGGCATCACTTGCAGA+AGG + chr5.4:67699265-67699284 MS.gene070505:CDS 50.0%
GTTTGCCACCGAGTCCATAT+CGG - chr5.4:67697023-67697042 None:intergenic 50.0%
TCAAGCATCTCTGTGGGATC+AGG - chr5.4:67697688-67697707 None:intergenic 50.0%
TGAATTCCCGAACCGGTCTT+CGG - chr5.4:67696511-67696530 None:intergenic 50.0%
TGACGAATCCCAATCTGACG+GGG + chr5.4:67698592-67698611 MS.gene070505:CDS 50.0%
TGGACTGCACTGAAATCTGC+TGG + chr5.4:67698494-67698513 MS.gene070505:CDS 50.0%
TGGAGACACCCCTTTGCATT+TGG + chr5.4:67697864-67697883 MS.gene070505:CDS 50.0%
! ACATAACCGAAGACCGGTTC+GGG + chr5.4:67696502-67696521 MS.gene070505:CDS 50.0%
! CAAGTGTTGCCCAAAAGAGG+AGG - chr5.4:67698994-67699013 None:intergenic 50.0%
! CTCCTCTTTTGGGCAACACT+TGG + chr5.4:67698992-67699011 MS.gene070505:CDS 50.0%
! TCCCAAGTGTTGCCCAAAAG+AGG - chr5.4:67698997-67699016 None:intergenic 50.0%
AACAATGATTCTCGGCCGGG+GGG + chr5.4:67696654-67696673 MS.gene070505:CDS 55.0%
CAACAATGATTCTCGGCCGG+GGG + chr5.4:67696653-67696672 MS.gene070505:CDS 55.0%
CAGGGTAGAGACACTCACCT+TGG + chr5.4:67698817-67698836 MS.gene070505:CDS 55.0%
CCAACAATGATTCTCGGCCG+GGG + chr5.4:67696652-67696671 MS.gene070505:CDS 55.0%
CCCAACAATGATTCTCGGCC+GGG + chr5.4:67696651-67696670 MS.gene070505:CDS 55.0%
CCCCAACAATGATTCTCGGC+CGG + chr5.4:67696650-67696669 MS.gene070505:CDS 55.0%
CCCGGCCGAGAATCATTGTT+GGG - chr5.4:67696654-67696673 None:intergenic 55.0%
CCGGCCGAGAATCATTGTTG+GGG - chr5.4:67696653-67696672 None:intergenic 55.0%
GTCAGATATTCCGGGACGGA+GGG - chr5.4:67698208-67698227 None:intergenic 55.0%
GTGGGTCAGATATTCCGGGA+CGG - chr5.4:67698212-67698231 None:intergenic 55.0%
! GGGCGGGGACAAGATTTTCA+AGG + chr5.4:67696467-67696486 MS.gene070505:CDS 55.0%
ACAATGATTCTCGGCCGGGG+GGG + chr5.4:67696655-67696674 MS.gene070505:CDS 60.0%
CCCCGGCCGAGAATCATTGT+TGG - chr5.4:67696655-67696674 None:intergenic 60.0%
GGTCAGATATTCCGGGACGG+AGG - chr5.4:67698209-67698228 None:intergenic 60.0%
GAAGCGTACTCCCTCCGTCC+CGG + chr5.4:67698195-67698214 MS.gene070505:intron 65.0%
GGGTATACCGCTGCACACCC+TGG + chr5.4:67697056-67697075 MS.gene070505:intron 65.0%
ACAACGGGCGATAGCCCCCC+CGG - chr5.4:67696672-67696691 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 67695880 67699291 67695880 ID=MS.gene070505
chr5.4 mRNA 67695880 67699291 67695880 ID=MS.gene070505.t1;Parent=MS.gene070505
chr5.4 exon 67695880 67696717 67695880 ID=MS.gene070505.t1.exon1;Parent=MS.gene070505.t1
chr5.4 CDS 67695880 67696717 67695880 ID=cds.MS.gene070505.t1;Parent=MS.gene070505.t1
chr5.4 exon 67697452 67698095 67697452 ID=MS.gene070505.t1.exon2;Parent=MS.gene070505.t1
chr5.4 CDS 67697452 67698095 67697452 ID=cds.MS.gene070505.t1;Parent=MS.gene070505.t1
chr5.4 exon 67698485 67699291 67698485 ID=MS.gene070505.t1.exon3;Parent=MS.gene070505.t1
chr5.4 CDS 67698485 67699291 67698485 ID=cds.MS.gene070505.t1;Parent=MS.gene070505.t1
Gene Sequence

>MS.gene070505

ATGCCTGTTTTTATTCCCGTACAAAATGAAGCCTTACCAAGGGATGTTACTGTTACTAATAAGAATCATAAACTTGAAATAATTGAATCCTCAGAAATAGTTGGGTTTCTTCCAAAATGTTGCAAATTTGATAATCAAATTGATTTGCTTAGTGAAGCATATCACTTGGTATATGGAGGTTCAACGAATTTGTTGGGTTCCTCTAATTTAACAGAAATTGAGACCCCTATGAAAAATACAGTGCTCCATATTGCAGCTTGGAATGGTAATAATGACATTGTGAATCTTGTAATTGAACGTGCTCCAAAACTTTTGTTCAAAGTTAACAAAAACAATGATAGTGCCTTACATGTTGCTGCTAGATGTGGACACATCTCAACCGTTAGAAAGCTATTAGCAAGTTATGCTAATATTCAACGGAGTGATATAAAAGTGGCATGGTTAGAATACACTAACAATTTGGATGACCCAAAAGACTACGATGAAAAGTCAAATATGGAGGATTTGTTAAAATTTGTGAAGATGGAGAATGTTCAAGGCAACACTATGTTACATGAGGCAATGTTATGTCGTGACATTAAGAGAATGGGCGGGGACAAGATTTTCAAGGTTTGTGAACTTTACATAACCGAAGACCGGTTCGGGAATTCATTGTCAAAGTGTTGTTATGAATATGCATTAGATATTGTTAACAAAGCAAAAAAATCAGTTCTTTACCTTGCTGTTGAAAATGAGGACAAGGATGCAGTCAAACTAATCTTGGAGAATTGCCCCAACAATGATTCTCGGCCGGGGGGGCTATCGCCCGTTGTAGAAGCAATCATGAAGCATAATCAAGGTATGTAATTAAATAGCTAAGTGATAATATTATTCTTAATAATTGATATTACTTTCAAAATTTACAATTTTGGAGAGAAAATTAATTTAATTTTTACTTATAGGGTCTTGCTAACCAGTGTCCTTAAGACAATGGTTAAGGAAATCAAAAGTAGTATATTTACCTTGAATTCAACAATGTTTTGACTTTTAAAAAATTAAATTCTCATTTTTTAATACTTCTCCTTAGCATTTCCCTTACTTATAATACTTCTTCCGGTTGCTATTACAAACAAATTCTCATTTTTTAAGTTTATTGATTAACCGATATGGACTCGGTGGCAAACCACATTGTATTGTGGGTATACCGCTGCACACCCTGGAATTATAAAAACTGCACTTATACCCTAATATAAATGTTATTTTGAATTCCCTGACTTAAAACATTTTGAACCGAGTGCCCAAATGCAAACAAACTCTTTCATTTGTTCCTTTTTGCACCGAGTTCTCTCTCTTACTCGTGTTTACTATGCTTCTGAAGATATAGAATGTTTTGACAAATGTTAGCTAACCCTCTTCTTTTTTGTTAGCTTTTTTTTTTTTTTTTTTTTGTTGAGGTTCTTAGAAATATTAATTATTTCTCAAATACTAATATCCAATTGATCAGAATGAATAAAGATTTTATGAGGCAGATTGCAATTATTAGGGGGGCGGCACTGATTTCATTATTTGGTAATATTTATGTTTATGTGTCAGAAATGCTCAGCATCATACTAGACAACAAGCCAACTTGGATCCATTTATGGGACACACATGAAAGGATTCCACTTCATTACGCAGCATCTGCAGGTTACCTTGAAGGTGTTGAATTATTACTTGGCATGTGCAAATGTTGCACAATGCAAATGGACAAATATGGCTATTACCCACTTCATCTAGCATCTTATGGTGGCCATGTGAAAGTGGTAGAAAAGTTGTTAGAATATTGTCCTGATCCCACAGAGATGCTTGACCTTTCTCTTGACCAAAATATTCTTCACGTTGCAGCAAACTATGGAAAACATGATGTGATACGATACATACTAGAAAGTCAAGTTCATGGACACATAAATCAGAAAGATAGTCGAGTTCGTGGACTCCGTCAGATGATAAATCAGAAAGATAAGAATGGAGACACCCCTTTGCATTTGGCTGCAAGATCATGTCATCCCACAACTGTGTTCTACTTAGTTAACCAAAATCTCGAAAAAGTGCATCTTGATATGGTTAACCAGAACAATGAAACGGCTCTTGACATTGTTAATTCGCTTTACCGGTTTGATAAATCATCATCTTTAAGACAGGCAAGTAGTTCTTTTATCCCCTCACACACTCATAAATTTATAAGTTACAGGTATATTGTAGAGTTTTTTATTTTTTTGACAACGATTATAAACTTCGGAGTTAAAACTTAAAAGTATAAAGTATAGATTTTGAAAAGAGAAACTAAAAAGTGCATTGAAGCGTACTCCCTCCGTCCCGGAATATCTGACCCACTTTCTAAAAAAAAGTGTCCCAAAATAACTGAGTCATTTCAATTTTCAATATAACTTTCATCATTCCCAATACAATATAACACTAAATAATACTACTTTTATCATTCCCAATACAATATATTCCATTTTCCCCTTTATTTATACATTTTTCTTAATCTGTGTGAAATGAGCCAAGGGCTCAGATAAATTGGGACGGAGGGAGTATAATATAACATAACATATTTTCTGACACATAGATGTAACAATGTTTGCAGCATCTTACATGGACTGCACTGAAATCTGCTGGTGCAAAGCTAAGTACAAGAAGAGCAGCAATCCATATTGAAGGCAATGATGAAGAATCCAAACCATCCAAACCAAGTGACGAATCCCAATCTGACGGGGAAAAGCAAAGTACAAGAAGAGCATCAATGTTATTCCCTATTAAATACACTGATGAAGAACCCGGACAACTTGAAGAATTCAAAAGGATTGAACCAAATAAAAGGAAAAGTAACGAATTGCACCAACAGAGTAAAGAAAAAGTCCCCGACAACACTGTACCAAATCTTTTTTTGGCGAAAGGCTCCAAGACTACATATAAAGACAGGGTAGAGACACTCACCTTGGTTTCAACACTTATAGTGACAGCATCAGTAGCAGCATGTTTAGCTGTTCCAGGTGAATCAGAAGGAAAAGCAAACAACCTATATCATGCAATGTTCCAAGTTTTCATTATCTTTATCACAATATCATTGTTCAGTTCCATTAGTTCTATTATCCTCCTCTTTTGGGCAACACTTGGGATAACTGAATTGGTGACTTTCTCTCTCAAAATCGTAATGCCGCTTCTTGGAATCGCTCTCATTTCACTATTTTTGGCTTTCATGGCTGGTATCTACACTGTTATCAGTGAACTTACTTGGTTGGCCAATGTTTTTCTGGTTATGATTGTGATTTTTGTTGTGGTTGTGTCTCTGTTGTACACTGTCCTTTTCCTTCCATCATCATCAACTAGAAAATCCATGCGATACATTTCCTATTACCCCTTTCGTGTTCTGGCATCACTTGCAGAAGGTTAA
Protein sequence

>MS.gene070505.t1

MPVFIPVQNEALPRDVTVTNKNHKLEIIESSEIVGFLPKCCKFDNQIDLLSEAYHLVYGGSTNLLGSSNLTEIETPMKNTVLHIAAWNGNNDIVNLVIERAPKLLFKVNKNNDSALHVAARCGHISTVRKLLASYANIQRSDIKVAWLEYTNNLDDPKDYDEKSNMEDLLKFVKMENVQGNTMLHEAMLCRDIKRMGGDKIFKVCELYITEDRFGNSLSKCCYEYALDIVNKAKKSVLYLAVENEDKDAVKLILENCPNNDSRPGGLSPVVEAIMKHNQEMLSIILDNKPTWIHLWDTHERIPLHYAASAGYLEGVELLLGMCKCCTMQMDKYGYYPLHLASYGGHVKVVEKLLEYCPDPTEMLDLSLDQNILHVAANYGKHDVIRYILESQVHGHINQKDSRVRGLRQMINQKDKNGDTPLHLAARSCHPTTVFYLVNQNLEKVHLDMVNQNNETALDIVNSLYRFDKSSSLRQASSSFIPSHTHKFISYRYIHLTWTALKSAGAKLSTRRAAIHIEGNDEESKPSKPSDESQSDGEKQSTRRASMLFPIKYTDEEPGQLEEFKRIEPNKRKSNELHQQSKEKVPDNTVPNLFLAKGSKTTYKDRVETLTLVSTLIVTASVAACLAVPGESEGKANNLYHAMFQVFIIFITISLFSSISSIILLFWATLGITELVTFSLKIVMPLLGIALISLFLAFMAGIYTVISELTWLANVFLVMIVIFVVVVSLLYTVLFLPSSSTRKSMRYISYYPFRVLASLAEG