AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070503

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070503.t1 MTR_5g085480 88.578 429 48 1 17 444 1 429 0.0 780
MS.gene070503.t1 MTR_2g033050 69.531 384 112 4 65 444 3 385 0.0 533
MS.gene070503.t1 MTR_5g085480 88.848 269 30 0 176 444 1 269 6.67e-176 503
MS.gene070503.t1 MTR_5g085830 61.628 430 148 7 18 441 41 459 4.04e-173 502
MS.gene070503.t1 MTR_5g085810 57.391 460 155 8 18 444 37 488 1.31e-171 499
MS.gene070503.t1 MTR_5g085490 69.288 267 76 4 9 270 25 290 4.52e-119 349
MS.gene070503.t1 MTR_5g085520 80.203 197 36 2 71 264 2 198 3.09e-105 311
MS.gene070503.t1 MTR_5g085500 71.338 157 45 0 288 444 2 158 1.23e-77 249
MS.gene070503.t1 MTR_5g085440 65.714 140 45 2 9 146 21 159 1.83e-50 175
MS.gene070503.t1 MTR_5g085530 79.452 73 14 1 266 337 1 73 4.80e-34 122
MS.gene070503.t1 MTR_1g069110 32.778 180 103 6 273 443 30 200 2.16e-14 75.5
MS.gene070503.t1 MTR_2g096190 28.676 272 171 6 172 440 26 277 1.81e-13 72.8
MS.gene070503.t1 MTR_7g100430 24.468 282 178 7 102 380 65 314 1.48e-12 70.1
MS.gene070503.t1 MTR_7g100390 29.412 221 146 5 222 439 46 259 1.71e-12 69.7
MS.gene070503.t1 MTR_4g062100 28.205 273 171 10 172 440 30 281 3.72e-12 68.6
MS.gene070503.t1 MTR_7g117620 25.478 314 176 11 102 411 40 299 2.20e-11 66.2
MS.gene070503.t1 MTR_2g096760 30.588 170 106 5 261 421 15 181 7.10e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070503.t1 AT1G03670 31.649 376 197 12 72 439 73 396 2.71e-39 150
MS.gene070503.t1 AT4G03500 30.556 396 224 9 50 439 53 403 2.31e-38 147
MS.gene070503.t1 AT4G03500 30.556 396 224 9 50 439 53 403 2.31e-38 147
MS.gene070503.t1 AT4G03500 30.808 396 223 10 50 439 76 426 2.41e-38 147
MS.gene070503.t1 AT4G03460 31.662 379 200 11 72 439 81 411 8.33e-37 142
MS.gene070503.t1 AT4G03460 30.166 421 230 13 35 439 7 379 5.39e-35 137
MS.gene070503.t1 AT4G03440 31.316 380 196 13 72 418 119 466 1.79e-33 134
MS.gene070503.t1 AT4G03440 31.316 380 196 13 72 418 119 466 1.79e-33 134
MS.gene070503.t1 AT4G03440 31.316 380 196 13 72 418 126 473 2.39e-33 133
MS.gene070503.t1 AT4G05040 31.143 350 190 10 66 405 110 418 2.60e-30 124
MS.gene070503.t1 AT4G05040 31.143 350 190 10 66 405 110 418 2.60e-30 124
MS.gene070503.t1 AT4G05040 31.143 350 190 10 66 405 110 418 2.60e-30 124
MS.gene070503.t1 AT4G05040 31.143 350 190 10 66 405 110 418 2.60e-30 124
MS.gene070503.t1 AT4G05040 31.143 350 190 10 66 405 110 418 2.60e-30 124
MS.gene070503.t1 AT4G05040 31.143 350 190 10 66 405 110 418 2.60e-30 124
MS.gene070503.t1 AT4G05040 31.143 350 190 10 66 405 110 418 2.60e-30 124
MS.gene070503.t1 AT4G03480 28.362 409 239 14 32 406 116 504 1.71e-29 122
MS.gene070503.t1 AT4G03480 28.155 412 233 14 32 406 116 501 2.12e-29 121
MS.gene070503.t1 AT4G03470 31.398 379 196 13 72 439 120 445 2.36e-29 121
MS.gene070503.t1 AT4G14390 28.205 390 222 10 62 439 123 466 6.17e-29 120
MS.gene070503.t1 AT4G14390 28.205 390 222 10 62 439 104 447 6.31e-29 120
MS.gene070503.t1 AT4G14390 28.205 390 222 10 62 439 104 447 1.10e-28 118
MS.gene070503.t1 AT4G03490 28.889 405 217 12 63 440 18 378 1.25e-28 119
MS.gene070503.t1 AT4G03490 28.889 405 217 12 63 440 28 388 1.31e-28 119
MS.gene070503.t1 AT4G14400 28.846 416 236 12 25 427 61 429 1.87e-28 119
MS.gene070503.t1 AT4G14390 28.133 391 222 10 62 439 123 467 2.08e-28 119
MS.gene070503.t1 AT4G03490 28.889 405 217 12 63 440 28 388 2.19e-28 118
MS.gene070503.t1 AT4G14400 29.787 376 210 11 65 427 29 363 8.30e-28 116
MS.gene070503.t1 AT4G14400 29.787 376 210 11 65 427 29 363 8.30e-28 116
MS.gene070503.t1 AT4G03450 29.333 375 220 12 70 439 70 404 4.37e-27 114
MS.gene070503.t1 AT4G03450 29.333 375 220 12 70 439 70 404 5.47e-27 114
MS.gene070503.t1 AT4G03490 28.723 376 199 11 63 411 18 351 1.64e-25 110
MS.gene070503.t1 AT4G03490 28.723 376 199 11 63 411 8 341 2.23e-25 109
MS.gene070503.t1 AT5G60070 28.889 225 147 5 222 440 70 287 1.04e-16 82.8
MS.gene070503.t1 AT1G07710 27.798 277 167 8 172 440 26 277 1.28e-13 73.2
MS.gene070503.t1 AT1G07710 23.735 257 168 6 165 417 58 290 9.65e-11 64.3
MS.gene070503.t1 AT1G07710 27.798 277 167 8 172 440 26 277 1.28e-13 73.2
MS.gene070503.t1 AT1G07710 23.735 257 168 6 165 417 58 290 9.65e-11 64.3
MS.gene070503.t1 AT1G10340 27.076 277 166 10 164 433 62 309 3.82e-13 72.0
MS.gene070503.t1 AT2G31820 27.469 324 195 10 127 439 105 399 2.78e-12 69.3
MS.gene070503.t1 AT5G02620 28.507 221 148 4 222 439 53 266 9.45e-12 67.4
MS.gene070503.t1 AT5G02620 28.507 221 148 4 222 439 53 266 9.45e-12 67.4
MS.gene070503.t1 AT5G02620 28.507 221 148 4 222 439 53 266 9.45e-12 67.4
MS.gene070503.t1 AT1G10340 27.076 277 162 11 164 433 62 305 1.90e-11 66.6
MS.gene070503.t1 AT2G24600 25.090 279 171 8 164 434 62 310 2.18e-11 66.2
MS.gene070503.t1 AT2G24600 25.090 279 171 8 164 434 62 310 2.18e-11 66.2
MS.gene070503.t1 AT2G24600 25.448 279 170 8 164 434 62 310 2.33e-11 66.2
MS.gene070503.t1 AT2G24600 25.448 279 170 8 164 434 62 310 2.33e-11 66.2

Find 87 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTACCTTGCGGTTGAAAA+TGG 0.143803 5.4:+67704913 MS.gene070503:CDS
AGCCAATTTGGATCCATTTA+AGG 0.162948 5.4:+67705153 MS.gene070503:CDS
TTTCATCTCTGTCCCTTAAA+TGG 0.204574 5.4:-67705166 None:intergenic
TACTTGAAAACAAGCCAATT+TGG 0.221391 5.4:+67705141 MS.gene070503:CDS
TATGGAACTTAAACTTGATA+TGG 0.230005 5.4:+67704276 MS.gene070503:CDS
CCAATTCATCTAGCATCTTA+TGG 0.233508 5.4:+67705296 MS.gene070503:CDS
CAAGAGCCGTTTCGTTATTC+TGG 0.269478 5.4:-67705607 None:intergenic
TCCCTTAAATGGATCCAAAT+TGG 0.297092 5.4:-67705155 None:intergenic
CCATAAGATGCTAGATGAAT+TGG 0.297882 5.4:-67705296 None:intergenic
TTGAATGCGAACAAAAGTTT+TGG 0.298979 5.4:-67704511 None:intergenic
CCTTGTTCACAAAACATAAT+AGG 0.300862 5.4:-67704711 None:intergenic
CTCAATTTCTAATAAATTAG+AGG 0.303256 5.4:-67704407 None:intergenic
TTTACCTTGCGGTTGAAAAT+GGG 0.345762 5.4:+67704914 MS.gene070503:CDS
CTACTTAGTTAACCAAAATA+AGG 0.348728 5.4:+67705559 MS.gene070503:CDS
AAAAGTGTCAAGCATGTCTC+TGG 0.352811 5.4:-67705366 None:intergenic
CTATGATGAGATGTTAAATA+TGG 0.357059 5.4:+67704684 MS.gene070503:CDS
GCCAATTTGGATCCATTTAA+GGG 0.359877 5.4:+67705154 MS.gene070503:CDS
TGGAGACACCCCTTTGCATT+TGG 0.361651 5.4:+67705505 MS.gene070503:CDS
TTCGGCTTAGCATCATTCTT+AGG 0.364848 5.4:-67704973 None:intergenic
CTTCATGATTGCTGCTACAA+CGG 0.366213 5.4:-67705007 None:intergenic
CTTCATTACGCAGCATCTAT+AGG 0.384519 5.4:+67705197 MS.gene070503:CDS
TCAAGCATGTCTCTGGGATC+TGG 0.393203 5.4:-67705359 None:intergenic
TTAGGACTATTCTCCAAGAT+TGG 0.395543 5.4:-67704955 None:intergenic
CTTCTTGATTTATCATCTGA+TGG 0.415177 5.4:-67705475 None:intergenic
GGAAGCAGTCAAACCAATCT+TGG 0.418295 5.4:+67704942 MS.gene070503:CDS
ATAAGTACTAGGAAAATTCC+TGG 0.422597 5.4:-67704254 None:intergenic
ATCTTGAAGCCAAATGCAAA+GGG 0.428458 5.4:-67705514 None:intergenic
CGCTTTACATGTTGCTGCAA+GGG 0.430475 5.4:+67704549 MS.gene070503:CDS
TGTTAATCATGCAAAGAAAT+CGG 0.432864 5.4:+67704888 MS.gene070503:CDS
GGAGGTTCAACGAATTTGTT+GGG 0.434049 5.4:+67704382 MS.gene070503:CDS
AGATGATAAATCAAGAAGAT+AGG 0.439765 5.4:+67705480 MS.gene070503:CDS
GAAGGTGTTGAACTATTACT+TGG 0.443121 5.4:+67705227 MS.gene070503:CDS
ACTAAGTAGTACACAGTTGC+GGG 0.444145 5.4:-67705545 None:intergenic
TGGAGGTTCAACGAATTTGT+TGG 0.445395 5.4:+67704381 MS.gene070503:CDS
TTCATGATTGCTGCTACAAC+GGG 0.446113 5.4:-67705006 None:intergenic
GTAATAGTTCAACACCTTCA+AGG 0.447605 5.4:-67705223 None:intergenic
CATAAGATGCTAGATGAATT+GGG 0.462698 5.4:-67705295 None:intergenic
TTAAGTTCCATATAAGTACT+AGG 0.470171 5.4:-67704265 None:intergenic
ACCGTTAAAACACTATTAGC+AGG 0.481857 5.4:+67704586 MS.gene070503:CDS
ACAATGCAAAGGGACCTCTA+TGG 0.489841 5.4:+67705266 MS.gene070503:CDS
GTGAACAAGGAGAATGCTAA+AGG 0.499459 5.4:+67704724 MS.gene070503:CDS
GCATCTATAGGTTACCTTGA+AGG 0.502099 5.4:+67705209 MS.gene070503:CDS
AACTTTACAAAACTAAAGAC+TGG 0.516209 5.4:+67704818 MS.gene070503:CDS
AATCACTGAGATCATTCCTT+CGG 0.523076 5.4:+67704309 MS.gene070503:CDS
TTACATATTGTAGCTTCGTA+TGG 0.542340 5.4:+67704451 MS.gene070503:CDS
GCAATCATGAAGCAGAATCA+AGG 0.549271 5.4:+67705018 MS.gene070503:CDS
AGACTGGTTAGAGAATTCAT+TGG 0.549590 5.4:+67704834 MS.gene070503:CDS
GCTTAGTGAAGCATATCACT+TGG 0.554255 5.4:+67704354 MS.gene070503:CDS
GCGCTTTACATGTTGCTGCA+AGG 0.556263 5.4:+67704548 MS.gene070503:CDS
TTACCTTGCGGTTGAAAATG+GGG 0.557584 5.4:+67704915 MS.gene070503:CDS
ATAGATGCTGCGTAATGAAG+TGG 0.557904 5.4:-67705194 None:intergenic
ATAAATCAAGAAGATAGGAA+TGG 0.561377 5.4:+67705485 MS.gene070503:CDS
TTACATGTTGCTGCAAGGGG+TGG 0.567511 5.4:+67704553 MS.gene070503:CDS
AATCTTGAAGCCAAATGCAA+AGG 0.568913 5.4:-67705515 None:intergenic
GAAGCATATCACTTGGTGCA+TGG 0.569308 5.4:+67704361 MS.gene070503:CDS
ACTTGATTGTCAAATTCCGA+AGG 0.570588 5.4:-67704325 None:intergenic
CTTCACATTGCAGCAAACCA+TGG 0.577396 5.4:+67705404 MS.gene070503:CDS
GGTTAGAATACACTAACAGT+TGG 0.579048 5.4:+67704647 MS.gene070503:CDS
AATGATCTCAGTGATTTCTG+AGG 0.579194 5.4:-67704302 None:intergenic
AACTAAGTAGTACACAGTTG+CGG 0.581933 5.4:-67705546 None:intergenic
TGTTAACCAGAATAACGAAA+CGG 0.582403 5.4:+67705601 MS.gene070503:CDS
GCTTTACATGTTGCTGCAAG+GGG 0.587253 5.4:+67704550 MS.gene070503:CDS
GAATTGGGAAATAGCCATAG+AGG 0.587573 5.4:-67705280 None:intergenic
ATGATCTCAGTGATTTCTGA+GGG 0.588604 5.4:-67704301 None:intergenic
ATGTTATGTGGCAAGAGCAA+TGG 0.593550 5.4:+67704769 MS.gene070503:CDS
CAAATGTTGCACAATGCAAA+GGG 0.596534 5.4:+67705256 MS.gene070503:CDS
TAAGAATGATGCTAAGCCGA+AGG 0.601334 5.4:+67704975 MS.gene070503:CDS
GCAAATGTTGCACAATGCAA+AGG 0.601707 5.4:+67705255 MS.gene070503:CDS
AAAGTGTCAAGCATGTCTCT+GGG 0.614057 5.4:-67705365 None:intergenic
GAAACGGCTCTTGACACTGT+TGG 0.615798 5.4:+67705617 MS.gene070503:CDS
AAGAATGATGCTAAGCCGAA+GGG 0.615867 5.4:+67704976 MS.gene070503:CDS
ACAACGGGCGATAATCCCTT+CGG 0.620725 5.4:-67704991 None:intergenic
CAGGTTATACTAATATTGAA+CGG 0.622382 5.4:+67704605 MS.gene070503:CDS
TTACATGAGGCAATGTTATG+TGG 0.625836 5.4:+67704757 MS.gene070503:CDS
TGATGAGATGTTAAATATGG+AGG 0.626199 5.4:+67704687 MS.gene070503:CDS
TATGTGGCAAGAGCAATGGT+AGG 0.627789 5.4:+67704773 MS.gene070503:CDS
ATTTAAGGGACAGAGATGAA+AGG 0.631487 5.4:+67705168 MS.gene070503:CDS
GAAATCGGTACTTTACCTTG+CGG 0.637275 5.4:+67704903 MS.gene070503:CDS
TGCGGTTGAAAATGGGGACA+AGG 0.640084 5.4:+67704921 MS.gene070503:CDS
AGCATCTTATGGTGGCCATG+TGG 0.645724 5.4:+67705307 MS.gene070503:CDS
AGGAAACACTATGTTACATG+AGG 0.655920 5.4:+67704744 MS.gene070503:CDS
TGAACTGTGATTTATCAGAG+TGG 0.660566 5.4:-67705641 None:intergenic
ACTTCTTCACTACTTCCACA+TGG 0.680029 5.4:-67705322 None:intergenic
ATGTGGCAAGAGCAATGGTA+GGG 0.694036 5.4:+67704774 MS.gene070503:CDS
ATTCATCTAGCATCTTATGG+TGG 0.698790 5.4:+67705299 MS.gene070503:CDS
GCATATCACTTGGTGCATGG+AGG 0.701663 5.4:+67704364 MS.gene070503:CDS
TCTTGAAGCCAAATGCAAAG+GGG 0.728698 5.4:-67705513 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAGATGTATAAATATAT+AGG - chr5.4:67705052-67705071 None:intergenic 10.0%
!! CTCAATTTCTAATAAATTAG+AGG - chr5.4:67704410-67704429 None:intergenic 20.0%
! AACTTTACAAAACTAAAGAC+TGG + chr5.4:67704818-67704837 MS.gene070503:CDS 25.0%
! AGATGATAAATCAAGAAGAT+AGG + chr5.4:67705480-67705499 MS.gene070503:CDS 25.0%
! ATAAATCAAGAAGATAGGAA+TGG + chr5.4:67705485-67705504 MS.gene070503:CDS 25.0%
! CAGGTTATACTAATATTGAA+CGG + chr5.4:67704605-67704624 MS.gene070503:CDS 25.0%
! CTACTTAGTTAACCAAAATA+AGG + chr5.4:67705559-67705578 MS.gene070503:CDS 25.0%
! CTATGATGAGATGTTAAATA+TGG + chr5.4:67704684-67704703 MS.gene070503:CDS 25.0%
! TATGGAACTTAAACTTGATA+TGG + chr5.4:67704276-67704295 MS.gene070503:CDS 25.0%
! TGAACGGTGTAATATAAAAA+TGG + chr5.4:67704621-67704640 MS.gene070503:CDS 25.0%
! TGTTAATCATGCAAAGAAAT+CGG + chr5.4:67704888-67704907 MS.gene070503:CDS 25.0%
! TTAAGTTCCATATAAGTACT+AGG - chr5.4:67704268-67704287 None:intergenic 25.0%
!! GAATTTTCCTAGTACTTATA+TGG + chr5.4:67704258-67704277 MS.gene070503:CDS 25.0%
ATAAGTACTAGGAAAATTCC+TGG - chr5.4:67704257-67704276 None:intergenic 30.0%
CATAAGATGCTAGATGAATT+GGG - chr5.4:67705298-67705317 None:intergenic 30.0%
CCTTGTTCACAAAACATAAT+AGG - chr5.4:67704714-67704733 None:intergenic 30.0%
CTTCTTGATTTATCATCTGA+TGG - chr5.4:67705478-67705497 None:intergenic 30.0%
GGTGTAATATAAAAATGGCA+TGG + chr5.4:67704626-67704645 MS.gene070503:CDS 30.0%
TACTTGAAAACAAGCCAATT+TGG + chr5.4:67705141-67705160 MS.gene070503:CDS 30.0%
TGATGAGATGTTAAATATGG+AGG + chr5.4:67704687-67704706 MS.gene070503:CDS 30.0%
TGTTAACCAGAATAACGAAA+CGG + chr5.4:67705601-67705620 MS.gene070503:CDS 30.0%
TTACATATTGTAGCTTCGTA+TGG + chr5.4:67704451-67704470 MS.gene070503:CDS 30.0%
! CCTATTATGTTTTGTGAACA+AGG + chr5.4:67704711-67704730 MS.gene070503:CDS 30.0%
! GTAACACTGTATTTTTCGTA+GGG - chr5.4:67704435-67704454 None:intergenic 30.0%
! TAACACTGTATTTTTCGTAG+GGG - chr5.4:67704434-67704453 None:intergenic 30.0%
! TGTAACACTGTATTTTTCGT+AGG - chr5.4:67704436-67704455 None:intergenic 30.0%
! TTGAATGCGAACAAAAGTTT+TGG - chr5.4:67704514-67704533 None:intergenic 30.0%
!! ACCTGCTAATAGTGTTTTAA+CGG - chr5.4:67704590-67704609 None:intergenic 30.0%
!!! GATGCACTTTTTCCTTATTT+TGG - chr5.4:67705574-67705593 None:intergenic 30.0%
!!! TGTACATTTCGATTTTTTGC+TGG + chr5.4:67704230-67704249 MS.gene070503:CDS 30.0%
AACTAAGTAGTACACAGTTG+CGG - chr5.4:67705549-67705568 None:intergenic 35.0%
AATCACTGAGATCATTCCTT+CGG + chr5.4:67704309-67704328 MS.gene070503:CDS 35.0%
AATCTTGAAGCCAAATGCAA+AGG - chr5.4:67705518-67705537 None:intergenic 35.0%
AATGATCTCAGTGATTTCTG+AGG - chr5.4:67704305-67704324 None:intergenic 35.0%
ACCGTTAAAACACTATTAGC+AGG + chr5.4:67704586-67704605 MS.gene070503:CDS 35.0%
ACTTGATTGTCAAATTCCGA+AGG - chr5.4:67704328-67704347 None:intergenic 35.0%
AGCCAATTTGGATCCATTTA+AGG + chr5.4:67705153-67705172 MS.gene070503:CDS 35.0%
AGGAAACACTATGTTACATG+AGG + chr5.4:67704744-67704763 MS.gene070503:CDS 35.0%
ATCTTGAAGCCAAATGCAAA+GGG - chr5.4:67705517-67705536 None:intergenic 35.0%
ATGATCTCAGTGATTTCTGA+GGG - chr5.4:67704304-67704323 None:intergenic 35.0%
ATTCATCTAGCATCTTATGG+TGG + chr5.4:67705299-67705318 MS.gene070503:CDS 35.0%
ATTTAAGGGACAGAGATGAA+AGG + chr5.4:67705168-67705187 MS.gene070503:CDS 35.0%
CAAATGTTGCACAATGCAAA+GGG + chr5.4:67705256-67705275 MS.gene070503:CDS 35.0%
CCAATTCATCTAGCATCTTA+TGG + chr5.4:67705296-67705315 MS.gene070503:CDS 35.0%
CCATAAGATGCTAGATGAAT+TGG - chr5.4:67705299-67705318 None:intergenic 35.0%
GCCAATTTGGATCCATTTAA+GGG + chr5.4:67705154-67705173 MS.gene070503:CDS 35.0%
GGTTAGAATACACTAACAGT+TGG + chr5.4:67704647-67704666 MS.gene070503:CDS 35.0%
GTAATAGTTCAACACCTTCA+AGG - chr5.4:67705226-67705245 None:intergenic 35.0%
TCCCTTAAATGGATCCAAAT+TGG - chr5.4:67705158-67705177 None:intergenic 35.0%
TTACATGAGGCAATGTTATG+TGG + chr5.4:67704757-67704776 MS.gene070503:CDS 35.0%
TTAGGACTATTCTCCAAGAT+TGG - chr5.4:67704958-67704977 None:intergenic 35.0%
TTTACCTTGCGGTTGAAAAT+GGG + chr5.4:67704914-67704933 MS.gene070503:CDS 35.0%
TTTCATCTCTGTCCCTTAAA+TGG - chr5.4:67705169-67705188 None:intergenic 35.0%
! AGACTGGTTAGAGAATTCAT+TGG + chr5.4:67704834-67704853 MS.gene070503:CDS 35.0%
! GTATCACATCATGTTTTCCA+TGG - chr5.4:67705424-67705443 None:intergenic 35.0%
!! GAAGGTGTTGAACTATTACT+TGG + chr5.4:67705227-67705246 MS.gene070503:CDS 35.0%
AAGAATGATGCTAAGCCGAA+GGG + chr5.4:67704976-67704995 MS.gene070503:CDS 40.0%
ACTAAGTAGTACACAGTTGC+GGG - chr5.4:67705548-67705567 None:intergenic 40.0%
ACTTCTTCACTACTTCCACA+TGG - chr5.4:67705325-67705344 None:intergenic 40.0%
ATAGATGCTGCGTAATGAAG+TGG - chr5.4:67705197-67705216 None:intergenic 40.0%
ATCTATATATCGAGCAGCAC+TGG + chr5.4:67705064-67705083 MS.gene070503:intron 40.0%
ATGTTATGTGGCAAGAGCAA+TGG + chr5.4:67704769-67704788 MS.gene070503:CDS 40.0%
CTTCATGATTGCTGCTACAA+CGG - chr5.4:67705010-67705029 None:intergenic 40.0%
CTTCATTACGCAGCATCTAT+AGG + chr5.4:67705197-67705216 MS.gene070503:CDS 40.0%
CTTTACCTTGCGGTTGAAAA+TGG + chr5.4:67704913-67704932 MS.gene070503:CDS 40.0%
GAAATCGGTACTTTACCTTG+CGG + chr5.4:67704903-67704922 MS.gene070503:CDS 40.0%
GAATTGGGAAATAGCCATAG+AGG - chr5.4:67705283-67705302 None:intergenic 40.0%
GCAAATGTTGCACAATGCAA+AGG + chr5.4:67705255-67705274 MS.gene070503:CDS 40.0%
GCAATCATGAAGCAGAATCA+AGG + chr5.4:67705018-67705037 MS.gene070503:CDS 40.0%
GCATCTATAGGTTACCTTGA+AGG + chr5.4:67705209-67705228 MS.gene070503:CDS 40.0%
GCTTAGTGAAGCATATCACT+TGG + chr5.4:67704354-67704373 MS.gene070503:CDS 40.0%
GTGAACAAGGAGAATGCTAA+AGG + chr5.4:67704724-67704743 MS.gene070503:CDS 40.0%
TAAGAATGATGCTAAGCCGA+AGG + chr5.4:67704975-67704994 MS.gene070503:CDS 40.0%
TCTTGAAGCCAAATGCAAAG+GGG - chr5.4:67705516-67705535 None:intergenic 40.0%
TGGAGGTTCAACGAATTTGT+TGG + chr5.4:67704381-67704400 MS.gene070503:CDS 40.0%
TTACCTTGCGGTTGAAAATG+GGG + chr5.4:67704915-67704934 MS.gene070503:CDS 40.0%
TTCATGATTGCTGCTACAAC+GGG - chr5.4:67705009-67705028 None:intergenic 40.0%
TTCGGCTTAGCATCATTCTT+AGG - chr5.4:67704976-67704995 None:intergenic 40.0%
! AAAGTGTCAAGCATGTCTCT+GGG - chr5.4:67705368-67705387 None:intergenic 40.0%
! GGAGGTTCAACGAATTTGTT+GGG + chr5.4:67704382-67704401 MS.gene070503:CDS 40.0%
!! AAAAGTGTCAAGCATGTCTC+TGG - chr5.4:67705369-67705388 None:intergenic 40.0%
!! GCAGCACTGGTTACATTATT+AGG + chr5.4:67705077-67705096 MS.gene070503:intron 40.0%
!!! TTTCGATTTTTTGCTGGACC+AGG + chr5.4:67704236-67704255 MS.gene070503:CDS 40.0%
ACAATGCAAAGGGACCTCTA+TGG + chr5.4:67705266-67705285 MS.gene070503:CDS 45.0%
ATGTGGCAAGAGCAATGGTA+GGG + chr5.4:67704774-67704793 MS.gene070503:CDS 45.0%
CAAGAGCCGTTTCGTTATTC+TGG - chr5.4:67705610-67705629 None:intergenic 45.0%
CGCTTTACATGTTGCTGCAA+GGG + chr5.4:67704549-67704568 MS.gene070503:CDS 45.0%
CTTCACATTGCAGCAAACCA+TGG + chr5.4:67705404-67705423 MS.gene070503:CDS 45.0%
GCTTTACATGTTGCTGCAAG+GGG + chr5.4:67704550-67704569 MS.gene070503:CDS 45.0%
GGAAGCAGTCAAACCAATCT+TGG + chr5.4:67704942-67704961 MS.gene070503:CDS 45.0%
TATGTGGCAAGAGCAATGGT+AGG + chr5.4:67704773-67704792 MS.gene070503:CDS 45.0%
!! GAAGCATATCACTTGGTGCA+TGG + chr5.4:67704361-67704380 MS.gene070503:CDS 45.0%
ACAACGGGCGATAATCCCTT+CGG - chr5.4:67704994-67705013 None:intergenic 50.0%
AGCATCTTATGGTGGCCATG+TGG + chr5.4:67705307-67705326 MS.gene070503:CDS 50.0%
GAAACGGCTCTTGACACTGT+TGG + chr5.4:67705617-67705636 MS.gene070503:CDS 50.0%
GCGCTTTACATGTTGCTGCA+AGG + chr5.4:67704548-67704567 MS.gene070503:CDS 50.0%
TGCGGTTGAAAATGGGGACA+AGG + chr5.4:67704921-67704940 MS.gene070503:CDS 50.0%
TGGAGACACCCCTTTGCATT+TGG + chr5.4:67705505-67705524 MS.gene070503:CDS 50.0%
TTACATGTTGCTGCAAGGGG+TGG + chr5.4:67704553-67704572 MS.gene070503:CDS 50.0%
! TGTCCCCATTTTCAACCGCA+AGG - chr5.4:67704921-67704940 None:intergenic 50.0%
!! GCATATCACTTGGTGCATGG+AGG + chr5.4:67704364-67704383 MS.gene070503:CDS 50.0%
!! TCAAGCATGTCTCTGGGATC+TGG - chr5.4:67705362-67705381 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 67704229 67705649 67704229 ID=MS.gene070503
chr5.4 mRNA 67704229 67705649 67704229 ID=MS.gene070503.t1;Parent=MS.gene070503
chr5.4 exon 67704229 67705039 67704229 ID=MS.gene070503.t1.exon1;Parent=MS.gene070503.t1
chr5.4 CDS 67704229 67705039 67704229 ID=cds.MS.gene070503.t1;Parent=MS.gene070503.t1
chr5.4 exon 67705126 67705649 67705126 ID=MS.gene070503.t1.exon2;Parent=MS.gene070503.t1
chr5.4 CDS 67705126 67705649 67705126 ID=cds.MS.gene070503.t1;Parent=MS.gene070503.t1
Gene Sequence

>MS.gene070503

ATGTACATTTCGATTTTTTGCTGGACCAGGAATTTTCCTAGTACTTATATGGAACTTAAACTTGATATGGAACCCTCAGAAATCACTGAGATCATTCCTTCGGAATTTGACAATCAAGTTAATTTGCTTAGTGAAGCATATCACTTGGTGCATGGAGGTTCAACGAATTTGTTGGGTTCCTCTAATTTATTAGAAATTGAGACCCCTACGAAAAATACAGTGTTACATATTGTAGCTTCGTATGGTAAAGATGACATTGTGAATCTTGTAATTGAACGTGCTCCAAAACTTTTGTTCGCATTCAACAAAAACAATGATAGCGCTTTACATGTTGCTGCAAGGGGTGGACACATCTCAACCGTTAAAACACTATTAGCAGGTTATACTAATATTGAACGGTGTAATATAAAAATGGCATGGTTAGAATACACTAACAGTTGGAATGATTTAGAAGACTATGATGAGATGTTAAATATGGAGGACCTATTATGTTTTGTGAACAAGGAGAATGCTAAAGGAAACACTATGTTACATGAGGCAATGTTATGTGGCAAGAGCAATGGTAGGGATGAGATTTTCAATGTTTGTCAACTTTACAAAACTAAAGACTGGTTAGAGAATTCATTGGCAAATTGTTGTTATGAATTTGCGTTAGAAATTGTTAATCATGCAAAGAAATCGGTACTTTACCTTGCGGTTGAAAATGGGGACAAGGAAGCAGTCAAACCAATCTTGGAGAATAGTCCTAAGAATGATGCTAAGCCGAAGGGATTATCGCCCGTTGTAGCAGCAATCATGAAGCAGAATCAAGGTATATAGTCCTATATATTTATACATCTATATATCGAGCAGCACTGGTTACATTATTAGGTAGTAATACTTAATTTTTTGTGTCAGAAATACTAAGCATCATACTTGAAAACAAGCCAATTTGGATCCATTTAAGGGACAGAGATGAAAGGCTTCCACTTCATTACGCAGCATCTATAGGTTACCTTGAAGGTGTTGAACTATTACTTGGAATGTGCAAATGTTGCACAATGCAAAGGGACCTCTATGGCTATTTCCCAATTCATCTAGCATCTTATGGTGGCCATGTGGAAGTAGTGAAGAAGTTGTTAGAATATTGTCCAGATCCCAGAGACATGCTTGACACTTTTCTTCAACAAAATATTCTTCACATTGCAGCAAACCATGGAAAACATGATGTGATACGCTACATACTTGAAAATCACGTTCGTGAACACCATCAGATGATAAATCAAGAAGATAGGAATGGAGACACCCCTTTGCATTTGGCTTCAAGATTTTGTCATCCCGCAACTGTGTACTACTTAGTTAACCAAAATAAGGAAAAAGTGCATCTTGATATTGTTAACCAGAATAACGAAACGGCTCTTGACACTGTTGGTCCACTCTGA
Protein sequence

>MS.gene070503.t1

MYISIFCWTRNFPSTYMELKLDMEPSEITEIIPSEFDNQVNLLSEAYHLVHGGSTNLLGSSNLLEIETPTKNTVLHIVASYGKDDIVNLVIERAPKLLFAFNKNNDSALHVAARGGHISTVKTLLAGYTNIERCNIKMAWLEYTNSWNDLEDYDEMLNMEDLLCFVNKENAKGNTMLHEAMLCGKSNGRDEIFNVCQLYKTKDWLENSLANCCYEFALEIVNHAKKSVLYLAVENGDKEAVKPILENSPKNDAKPKGLSPVVAAIMKQNQEILSIILENKPIWIHLRDRDERLPLHYAASIGYLEGVELLLGMCKCCTMQRDLYGYFPIHLASYGGHVEVVKKLLEYCPDPRDMLDTFLQQNILHIAANHGKHDVIRYILENHVREHHQMINQEDRNGDTPLHLASRFCHPATVYYLVNQNKEKVHLDIVNQNNETALDTVGPL