AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070509

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070509.t1 MTR_5g085480 65.604 596 143 9 1 588 144 685 0.0 715
MS.gene070509.t1 MTR_5g085480 64.151 583 147 9 18 592 1 529 0.0 678
MS.gene070509.t1 MTR_5g085830 56.500 600 178 11 2 592 180 705 0.0 608
MS.gene070509.t1 MTR_5g085810 57.571 601 174 8 1 592 205 733 0.0 608
MS.gene070509.t1 MTR_5g085500 54.323 532 104 10 125 592 2 458 2.55e-175 505
MS.gene070509.t1 MTR_2g033050 69.836 305 77 6 1 293 98 399 1.60e-143 422
MS.gene070509.t1 MTR_2g033045 75.625 160 39 0 434 593 60 219 1.05e-73 238
MS.gene070509.t1 MTR_5g085440 73.292 161 42 1 434 593 194 354 5.42e-69 228
MS.gene070509.t1 MTR_5g085520 75.000 108 20 1 1 101 91 198 6.47e-47 164
MS.gene070509.t1 MTR_5g085490 64.103 117 34 2 1 109 176 292 1.88e-38 144
MS.gene070509.t1 MTR_5g085530 83.562 73 11 1 103 174 1 73 8.96e-36 129
MS.gene070509.t1 MTR_5g085560 39.130 161 51 4 301 460 2 116 2.40e-20 87.8
MS.gene070509.t1 MTR_2g101310 21.443 485 329 11 101 567 145 595 1.46e-17 86.7
MS.gene070509.t1 MTR_5g043640 32.895 152 92 3 124 273 50 193 6.30e-14 72.4
MS.gene070509.t1 MTR_5g043640 32.836 134 82 4 59 187 51 181 3.36e-11 64.3
MS.gene070509.t1 MTR_1g069110 23.327 493 253 19 110 567 30 432 1.12e-13 73.9
MS.gene070509.t1 MTR_2g101320 21.971 487 331 10 101 570 145 599 3.29e-13 73.2
MS.gene070509.t1 MTR_7g100390 27.602 221 150 4 59 275 46 260 3.34e-13 72.8
MS.gene070509.t1 MTR_8g072510 24.887 221 154 3 69 278 13 232 1.34e-12 70.5
MS.gene070509.t1 MTR_2g101310 24.348 230 152 5 101 325 145 357 1.61e-12 70.5
MS.gene070509.t1 MTR_2g096190 23.028 317 209 8 2 314 53 338 2.02e-12 70.5
MS.gene070509.t1 MTR_2g096190 21.839 522 309 17 66 558 31 482 4.35e-12 69.3
MS.gene070509.t1 MTR_4g062100 25.926 216 141 5 10 221 65 265 5.87e-12 68.9
MS.gene070509.t1 MTR_8g069880 28.017 232 152 5 65 284 6 234 8.80e-12 68.2
MS.gene070509.t1 MTR_8g105260 28.017 232 156 5 66 292 67 292 1.95e-11 67.0
MS.gene070509.t1 MTR_8g467780 30.556 180 107 5 113 275 62 240 2.06e-11 66.6
MS.gene070509.t1 MTR_7g117620 28.319 226 150 6 55 275 74 292 4.68e-11 65.9
MS.gene070509.t1 MTR_7g117620 28.859 149 98 3 108 255 164 305 7.29e-11 65.5
MS.gene070509.t1 MTR_8g073260 26.744 258 160 6 66 312 11 250 4.89e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070509.t1 AT4G03460 36.861 274 155 6 50 314 148 412 7.07e-42 160
MS.gene070509.t1 AT4G03460 25.146 513 293 15 124 591 123 589 5.11e-19 91.3
MS.gene070509.t1 AT4G03460 36.861 274 155 6 50 314 180 444 1.14e-41 159
MS.gene070509.t1 AT4G03460 24.958 597 344 17 53 591 71 621 4.31e-21 97.8
MS.gene070509.t1 AT4G03500 36.900 271 156 7 54 320 206 465 1.57e-39 154
MS.gene070509.t1 AT4G03500 36.145 166 99 2 434 593 474 638 1.33e-20 96.7
MS.gene070509.t1 AT4G03500 36.900 271 156 7 54 320 183 442 1.59e-39 153
MS.gene070509.t1 AT4G03500 36.145 166 99 2 434 593 451 615 1.17e-20 96.7
MS.gene070509.t1 AT4G03500 36.900 271 156 7 54 320 183 442 1.59e-39 153
MS.gene070509.t1 AT4G03500 36.145 166 99 2 434 593 451 615 1.17e-20 96.7
MS.gene070509.t1 AT1G03670 34.875 281 161 9 59 330 177 444 1.42e-37 147
MS.gene070509.t1 AT1G03670 35.329 167 100 3 434 593 445 610 4.49e-21 97.8
MS.gene070509.t1 AT4G03490 32.121 330 192 12 11 335 130 432 2.22e-32 132
MS.gene070509.t1 AT4G03490 23.492 630 368 21 14 591 25 592 1.02e-24 109
MS.gene070509.t1 AT4G03490 32.121 330 192 12 11 335 140 442 2.57e-32 132
MS.gene070509.t1 AT4G03490 23.492 630 368 21 14 591 35 602 1.41e-24 108
MS.gene070509.t1 AT4G03490 33.115 305 173 11 12 312 141 418 4.33e-32 132
MS.gene070509.t1 AT4G03490 22.727 616 354 21 14 575 35 582 1.11e-19 93.6
MS.gene070509.t1 AT4G05040 33.333 273 153 6 36 283 190 458 1.62e-28 120
MS.gene070509.t1 AT4G05040 33.333 273 153 6 36 283 190 458 1.62e-28 120
MS.gene070509.t1 AT4G05040 33.333 273 153 6 36 283 190 458 1.62e-28 120
MS.gene070509.t1 AT4G05040 33.333 273 153 6 36 283 190 458 1.62e-28 120
MS.gene070509.t1 AT4G05040 33.333 273 153 6 36 283 190 458 1.62e-28 120
MS.gene070509.t1 AT4G05040 33.333 273 153 6 36 283 190 458 1.62e-28 120
MS.gene070509.t1 AT4G05040 33.333 273 153 6 36 283 190 458 1.62e-28 120
MS.gene070509.t1 AT4G03470 35.833 240 136 7 52 278 215 449 1.52e-26 115
MS.gene070509.t1 AT4G03490 34.298 242 136 8 11 249 130 351 9.57e-25 109
MS.gene070509.t1 AT4G03490 23.519 591 342 22 14 575 25 534 1.08e-20 96.7
MS.gene070509.t1 AT4G03490 33.471 242 138 8 11 249 120 341 1.37e-24 108
MS.gene070509.t1 AT4G03490 23.519 591 342 22 14 575 15 524 1.09e-20 97.1
MS.gene070509.t1 AT4G03480 35.678 199 111 6 57 243 307 500 4.24e-24 107
MS.gene070509.t1 AT4G03480 35.678 199 111 6 57 243 310 503 4.92e-24 107
MS.gene070509.t1 AT4G03450 36.869 198 117 5 91 283 219 413 2.14e-23 105
MS.gene070509.t1 AT4G03450 36.869 198 117 5 91 283 219 413 3.53e-23 104
MS.gene070509.t1 AT4G03440 34.928 209 123 5 57 255 267 472 2.85e-22 102
MS.gene070509.t1 AT4G03440 29.932 147 91 3 429 568 545 686 2.82e-11 67.0
MS.gene070509.t1 AT4G03440 34.928 209 123 5 57 255 260 465 2.89e-22 102
MS.gene070509.t1 AT4G03440 29.932 147 91 3 429 568 538 679 2.96e-11 67.0
MS.gene070509.t1 AT4G03440 34.928 209 123 5 57 255 260 465 2.89e-22 102
MS.gene070509.t1 AT4G03440 29.932 147 91 3 429 568 538 679 2.96e-11 67.0
MS.gene070509.t1 AT4G14390 29.434 265 158 6 36 275 207 467 1.75e-20 96.3
MS.gene070509.t1 AT4G14390 29.434 265 158 6 36 275 188 448 1.83e-20 96.3
MS.gene070509.t1 AT4G14390 29.434 265 158 6 36 275 188 448 2.71e-20 95.1
MS.gene070509.t1 AT4G14390 29.323 266 158 6 36 275 207 468 5.78e-20 94.7
MS.gene070509.t1 AT4G14400 30.962 239 147 7 59 283 214 448 1.16e-18 90.5
MS.gene070509.t1 AT4G14400 30.962 239 147 7 59 283 148 382 1.20e-18 90.1
MS.gene070509.t1 AT4G14400 30.962 239 147 7 59 283 148 382 1.20e-18 90.1
MS.gene070509.t1 AT5G02620 29.646 226 147 6 55 275 49 267 4.95e-15 78.6
MS.gene070509.t1 AT5G02620 29.646 226 147 6 55 275 49 267 4.95e-15 78.6
MS.gene070509.t1 AT5G02620 29.646 226 147 6 55 275 49 267 4.95e-15 78.6
MS.gene070509.t1 AT5G60070 22.426 544 317 15 53 559 23 498 8.16e-15 77.8
MS.gene070509.t1 AT5G60070 22.941 340 218 11 2 327 60 369 5.50e-11 65.9
MS.gene070509.t1 AT2G03430 33.333 156 92 5 121 273 40 186 4.72e-13 69.7
MS.gene070509.t1 AT5G02620 29.231 195 127 5 55 245 49 236 8.50e-13 71.6
MS.gene070509.t1 AT5G02620 29.231 195 127 5 55 245 49 236 8.50e-13 71.6
MS.gene070509.t1 AT1G07710 26.667 255 159 8 7 255 58 290 1.85e-12 70.5
MS.gene070509.t1 AT1G07710 26.667 255 159 8 7 255 58 290 1.85e-12 70.5
MS.gene070509.t1 AT2G01680 28.351 194 122 5 118 310 118 295 7.72e-12 68.6
MS.gene070509.t1 AT5G51160 33.333 120 77 3 125 243 44 161 3.05e-11 66.2

Find 104 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGTTGGCCAATCTCTTTC+TGG 0.069314 5.4:+67655269 MS.gene070509:CDS
GTAATAATTCAACACCTTTA+AGG 0.143010 5.4:-67653998 None:intergenic
CTTTACCTTGCGGTTGAAAA+TGG 0.143803 5.4:+67653177 MS.gene070509:CDS
AACCAACTTGGATCCATTTA+AGG 0.162212 5.4:+67653928 MS.gene070509:CDS
GTGCTACTATCCTCCTCTTT+TGG 0.174524 5.4:+67655118 MS.gene070509:CDS
TTTCATCTTTGTCCCTTAAA+TGG 0.179525 5.4:-67653941 None:intergenic
CCAATTCATCTAGCATCTTT+TGG 0.223821 5.4:+67654071 MS.gene070509:CDS
AAAGATCAAGTTAAAGTAAA+AGG 0.237947 5.4:+67654832 MS.gene070509:CDS
CTATGTTTATATGTGGTGTT+AGG 0.258586 5.4:-67654934 None:intergenic
ATATGGAGTGCCACTCTTCT+TGG 0.263855 5.4:-67654577 None:intergenic
TTACATGAGGCAATATTATA+TGG 0.267434 5.4:+67653036 MS.gene070509:CDS
CCAAAAGATGCTAGATGAAT+TGG 0.268431 5.4:-67654071 None:intergenic
CATTTGATTTGGATTCAATA+TGG 0.272408 5.4:-67654594 None:intergenic
CTACTGATGCTGTCACTATA+AGG 0.275406 5.4:-67654984 None:intergenic
AAAATTGTAATGCCGCTTCT+TGG 0.277977 5.4:+67655180 MS.gene070509:CDS
GCATCTATAGGTTACCTTAA+AGG 0.307501 5.4:+67653984 MS.gene070509:CDS
CACGAAGAATCCAAACAAAA+TGG 0.319597 5.4:+67654667 MS.gene070509:CDS
TGAATTTGAATCATTTGATT+TGG 0.324648 5.4:-67654605 None:intergenic
ACAATTCAAAGGGACAAATA+TGG 0.343812 5.4:+67654041 MS.gene070509:CDS
TTTACCTTGCGGTTGAAAAT+GGG 0.345762 5.4:+67653178 MS.gene070509:CDS
CCTTGTTCACAAAACATAAC+AGG 0.357712 5.4:-67652990 None:intergenic
CTGTTATCAGTGAACTTACT+TGG 0.359026 5.4:+67655250 MS.gene070509:CDS
TGGAGACACCCCTTTGCATT+TGG 0.361651 5.4:+67654283 MS.gene070509:CDS
AACACTTGGATTAACTGAAT+TGG 0.367849 5.4:+67655143 MS.gene070509:CDS
TAGGGTAGAGACACTGATAT+TGG 0.378393 5.4:+67654954 MS.gene070509:CDS
TCCGGCTTATCATCATTCTC+AGG 0.379745 5.4:-67653237 None:intergenic
CTTCATTACGCAGCATCTAT+AGG 0.384519 5.4:+67653972 MS.gene070509:CDS
TGTTAATCATGCAAAGAAAT+CGG 0.385880 5.4:+67653152 MS.gene070509:CDS
GAGGAGGATAGTAGCACTAA+TGG 0.407541 5.4:-67655112 None:intergenic
AGATGATAAATCAGAAAGAT+AGG 0.425002 5.4:+67654258 MS.gene070509:CDS
AATGTTTGCAGCATCTTACA+TGG 0.427993 5.4:+67654521 MS.gene070509:intron
GAGATTTGGTACAGTGTTCT+CGG 0.428269 5.4:-67654896 None:intergenic
GCAGCATGTTTAGCTGTTCC+AGG 0.429250 5.4:+67655006 MS.gene070509:CDS
ACCAACTTGGATCCATTTAA+GGG 0.432434 5.4:+67653929 MS.gene070509:CDS
GGATGCAGTCAAATTAATCT+TGG 0.441127 5.4:+67653206 MS.gene070509:CDS
ACAAAACTACAGATCGGTTC+GGG 0.445623 5.4:+67653088 MS.gene070509:CDS
CTTCATGATAGCTGCTACAA+CGG 0.446936 5.4:-67653271 None:intergenic
TCAAGCATCTCTGTGGGATC+AGG 0.447170 5.4:-67654134 None:intergenic
CAAAAGATGCTAGATGAATT+GGG 0.455878 5.4:-67654070 None:intergenic
TATGTTTATATGTGGTGTTA+GGG 0.471965 5.4:-67654933 None:intergenic
CTTTCTGATTTATCATCTGA+CGG 0.473876 5.4:-67654253 None:intergenic
ATCTTGCAGCCAAATGCAAA+GGG 0.474929 5.4:-67654292 None:intergenic
TACAAAACTACAGATCGGTT+CGG 0.478216 5.4:+67653087 MS.gene070509:CDS
TACATGAGGCAATATTATAT+GGG 0.481098 5.4:+67653037 MS.gene070509:CDS
TACTGATGCTGTCACTATAA+GGG 0.482396 5.4:-67654983 None:intergenic
CAAACAAAATGGAACAGAAC+AGG 0.483861 5.4:+67654678 MS.gene070509:CDS
TAGAACAAGAAAGGGGTAGT+AGG 0.486192 5.4:-67655385 None:intergenic
CAAGAGCCGTTTCATTATTG+TGG 0.486825 5.4:-67654376 None:intergenic
GAACTTTGAAGTTGATGATG+AGG 0.491539 5.4:-67655349 None:intergenic
AAATAAAGCCGCAGATTACA+AGG 0.502209 5.4:+67654855 MS.gene070509:CDS
TACTAGAAAACAAACCAACT+TGG 0.508083 5.4:+67653916 MS.gene070509:CDS
GCAAATGCTGCACAATTCAA+AGG 0.508451 5.4:+67654030 MS.gene070509:CDS
TTCATGATAGCTGCTACAAC+GGG 0.508650 5.4:-67653270 None:intergenic
AGTGATGCTAGAACAAGAAA+GGG 0.509157 5.4:-67655393 None:intergenic
ATCCAAGTGTTGCCCAAAAG+AGG 0.511836 5.4:-67655131 None:intergenic
TCCCTTAAATGGATCCAAGT+TGG 0.512343 5.4:-67653930 None:intergenic
CTCTACCCTATGTTTATATG+TGG 0.513107 5.4:-67654941 None:intergenic
GGTAACAGCCAGAAAGAGAT+TGG 0.513913 5.4:-67655277 None:intergenic
AACTTTGAAGTTGATGATGA+GGG 0.515494 5.4:-67655348 None:intergenic
AAGTGATGCTAGAACAAGAA+AGG 0.516385 5.4:-67655394 None:intergenic
GGTTAACCACAATAATGAAA+CGG 0.517409 5.4:+67654370 MS.gene070509:CDS
ACTAAGTAGCACACAGTTGT+GGG 0.517636 5.4:-67654323 None:intergenic
CTAACACCACATATAAACAT+AGG 0.522398 5.4:+67654935 MS.gene070509:CDS
TATCAGTGAACTTACTTGGT+TGG 0.523227 5.4:+67655254 MS.gene070509:CDS
AGATTTGGTACAGTGTTCTC+GGG 0.525174 5.4:-67654895 None:intergenic
CTTGAAACATTGCATGACAT+AGG 0.526678 5.4:-67655053 None:intergenic
CCCTGAGAATGATGATAAGC+CGG 0.526814 5.4:+67653236 MS.gene070509:CDS
AACTAAGTAGCACACAGTTG+TGG 0.528967 5.4:-67654324 None:intergenic
TAATAAATCATCTTTAAGAC+AGG 0.533175 5.4:+67654424 MS.gene070509:CDS
CAAATGCTGCACAATTCAAA+GGG 0.544446 5.4:+67654031 MS.gene070509:CDS
CTTCACATTGCAGCAAACCA+TGG 0.545880 5.4:+67654179 MS.gene070509:CDS
GTTCTAGCATCACTTGCAGA+AGG 0.548456 5.4:+67655402 MS.gene070509:CDS
GCTATCATGAAGCAGAATCA+AGG 0.549976 5.4:+67653282 MS.gene070509:CDS
TTACCTTGCGGTTGAAAATG+GGG 0.557584 5.4:+67653179 MS.gene070509:CDS
ATAGATGCTGCGTAATGAAG+TGG 0.557904 5.4:-67653969 None:intergenic
GTGAACAAGGAGAATGCTCA+AGG 0.562389 5.4:+67653003 MS.gene070509:CDS
TGGACTGCACTGAAATCTGC+TGG 0.569090 5.4:+67654541 MS.gene070509:CDS
AACGTTACAAAACTACAGAT+CGG 0.569138 5.4:+67653082 MS.gene070509:CDS
ATAAATCAGAAAGATAGGAA+TGG 0.570805 5.4:+67654263 MS.gene070509:CDS
GATCTTGCAGCCAAATGCAA+AGG 0.573294 5.4:-67654293 None:intergenic
TTGAAGTTGATGATGAGGGA+AGG 0.573464 5.4:-67655344 None:intergenic
ATGAGAGCAGTTCCAAGAAG+CGG 0.583768 5.4:-67655192 None:intergenic
TTGATGATGAGGGAAGGAAA+AGG 0.584921 5.4:-67655338 None:intergenic
CCGGCTTATCATCATTCTCA+GGG 0.589724 5.4:-67653236 None:intergenic
TTCTAGCATCACTTGCAGAA+GGG 0.592149 5.4:+67655403 MS.gene070509:CDS
TAACACCACATATAAACATA+GGG 0.596060 5.4:+67654936 MS.gene070509:CDS
TGTTGTCATGTAAATATCTG+AGG 0.599342 5.4:-67654776 None:intergenic
GCTGTTCCAGGTGAAGCAGA+AGG 0.600903 5.4:+67655018 MS.gene070509:CDS
ATTTAAGGGACAAAGATGAA+AGG 0.606400 5.4:+67653943 MS.gene070509:CDS
ACAACGGGTGATAGTCCCTC+CGG 0.611767 5.4:-67653255 None:intergenic
TGTTGTGTCCTTGTAATCTG+CGG 0.613410 5.4:-67654863 None:intergenic
TGATGAGGGAAGGAAAAGGA+CGG 0.615778 5.4:-67655334 None:intergenic
TGCGGTTGAAAATGGGGACA+AGG 0.619936 5.4:+67653185 MS.gene070509:CDS
AAAAGTGTCAAGCATCTCTG+TGG 0.622563 5.4:-67654141 None:intergenic
AAAGTGTCAAGCATCTCTGT+GGG 0.628305 5.4:-67654140 None:intergenic
GAAATCGGTACTTTACCTTG+CGG 0.637275 5.4:+67653167 MS.gene070509:CDS
ACATGAGGCAATATTATATG+GGG 0.640901 5.4:+67653038 MS.gene070509:CDS
CAAGTGTTGCCCAAAAGAGG+AGG 0.641422 5.4:-67655128 None:intergenic
TGAGAATGATGATAAGCCGG+AGG 0.661002 5.4:+67653239 MS.gene070509:CDS
AAAGCAAAGTCCAAGAAGAG+TGG 0.661770 5.4:+67654567 MS.gene070509:CDS
AGGAAACACTATGTTACATG+AGG 0.662740 5.4:+67653023 MS.gene070509:CDS
GAGAATGATGATAAGCCGGA+GGG 0.668890 5.4:+67653240 MS.gene070509:CDS
GTGATGCTAGAACAAGAAAG+GGG 0.722450 5.4:-67655392 None:intergenic
TCTTGCAGCCAAATGCAAAG+GGG 0.746892 5.4:-67654291 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAAGATAGAAAATAAATAA+AGG - chr5.4:67653567-67653586 None:intergenic 15.0%
!! AAAGATCAAGTTAAAGTAAA+AGG + chr5.4:67654832-67654851 MS.gene070509:CDS 20.0%
!! AACCCATTTAATAAACAAAA+AGG - chr5.4:67653394-67653413 None:intergenic 20.0%
!! ACCCATTTAATAAACAAAAA+GGG - chr5.4:67653393-67653412 None:intergenic 20.0%
!! ACTAATATTACAAAGAACTA+GGG - chr5.4:67653789-67653808 None:intergenic 20.0%
!! ATATTCTATATATCTTCCTT+CGG - chr5.4:67653748-67653767 None:intergenic 20.0%
!! GGAAATAGTAAAAAAATTGT+TGG + chr5.4:67654103-67654122 MS.gene070509:CDS 20.0%
!! GTATGAGTTAATTACATTAT+TGG + chr5.4:67653854-67653873 MS.gene070509:intron 20.0%
!! TGAATTTGAATCATTTGATT+TGG - chr5.4:67654608-67654627 None:intergenic 20.0%
!! TTATTTATTGAAAACAACGA+TGG - chr5.4:67653492-67653511 None:intergenic 20.0%
!!! AATGTATTGACATAAACTAT+TGG - chr5.4:67653420-67653439 None:intergenic 20.0%
!!! ACCCTTTTTGTTTATTAAAT+GGG + chr5.4:67653389-67653408 MS.gene070509:intron 20.0%
!!! TAATAAATCATCTTTAAGAC+AGG + chr5.4:67654424-67654443 MS.gene070509:CDS 20.0%
! ACAACAGAAAAAAAGAAAAC+AGG + chr5.4:67654796-67654815 MS.gene070509:CDS 25.0%
! AGATGATAAATCAGAAAGAT+AGG + chr5.4:67654258-67654277 MS.gene070509:CDS 25.0%
! ATAAATCAGAAAGATAGGAA+TGG + chr5.4:67654263-67654282 MS.gene070509:CDS 25.0%
! GACTAATATTACAAAGAACT+AGG - chr5.4:67653790-67653809 None:intergenic 25.0%
! GTAATAATTCAACACCTTTA+AGG - chr5.4:67654001-67654020 None:intergenic 25.0%
! TAACACCACATATAAACATA+GGG + chr5.4:67654936-67654955 MS.gene070509:CDS 25.0%
! TACATGAGGCAATATTATAT+GGG + chr5.4:67653037-67653056 MS.gene070509:CDS 25.0%
! TGTATTTAAATATCAACCGA+AGG + chr5.4:67653729-67653748 MS.gene070509:intron 25.0%
! TGTTAATCATGCAAAGAAAT+CGG + chr5.4:67653152-67653171 MS.gene070509:CDS 25.0%
! TTACATGAGGCAATATTATA+TGG + chr5.4:67653036-67653055 MS.gene070509:CDS 25.0%
!! TATGTTTATATGTGGTGTTA+GGG - chr5.4:67654936-67654955 None:intergenic 25.0%
!!! ATTTTTTTACTATTTCCACG+TGG - chr5.4:67654100-67654119 None:intergenic 25.0%
!!! CACCCTTTTTGTTTATTAAA+TGG + chr5.4:67653388-67653407 MS.gene070509:intron 25.0%
!!! CATTTGATTTGGATTCAATA+TGG - chr5.4:67654597-67654616 None:intergenic 25.0%
!!! CTAGCAACATTTGTTATTTT+CGG - chr5.4:67653829-67653848 None:intergenic 25.0%
!!! TTCTCTTGTTCTATTTTTTC+TGG - chr5.4:67654631-67654650 None:intergenic 25.0%
AAATAACAAATGTTGCTAGC+TGG + chr5.4:67653830-67653849 MS.gene070509:intron 30.0%
AACACTTGGATTAACTGAAT+TGG + chr5.4:67655143-67655162 MS.gene070509:CDS 30.0%
AACGTTACAAAACTACAGAT+CGG + chr5.4:67653082-67653101 MS.gene070509:CDS 30.0%
ACAATTCAAAGGGACAAATA+TGG + chr5.4:67654041-67654060 MS.gene070509:CDS 30.0%
ACATGAGGCAATATTATATG+GGG + chr5.4:67653038-67653057 MS.gene070509:CDS 30.0%
ATTTAAGGGACAAAGATGAA+AGG + chr5.4:67653943-67653962 MS.gene070509:CDS 30.0%
CAAAAGATGCTAGATGAATT+GGG - chr5.4:67654073-67654092 None:intergenic 30.0%
CTAACACCACATATAAACAT+AGG + chr5.4:67654935-67654954 MS.gene070509:CDS 30.0%
CTACTTAGTTAACCAAAAAG+TGG + chr5.4:67654337-67654356 MS.gene070509:CDS 30.0%
CTATGTTTATATGTGGTGTT+AGG - chr5.4:67654937-67654956 None:intergenic 30.0%
CTTTCTGATTTATCATCTGA+CGG - chr5.4:67654256-67654275 None:intergenic 30.0%
GGTTAACCACAATAATGAAA+CGG + chr5.4:67654370-67654389 MS.gene070509:CDS 30.0%
TACTAGAAAACAAACCAACT+TGG + chr5.4:67653916-67653935 MS.gene070509:CDS 30.0%
TGTTGTCATGTAAATATCTG+AGG - chr5.4:67654779-67654798 None:intergenic 30.0%
TTTATTGAAAACAACGATGG+TGG - chr5.4:67653489-67653508 None:intergenic 30.0%
TTTCATCTTTGTCCCTTAAA+TGG - chr5.4:67653944-67653963 None:intergenic 30.0%
!! AACTTTGAAGTTGATGATGA+GGG - chr5.4:67655351-67655370 None:intergenic 30.0%
!! TTTTGAGAGCTAATTTGTGT+AGG + chr5.4:67653684-67653703 MS.gene070509:intron 30.0%
!!! AAAGGGTGTTTTTTAGACAA+AGG - chr5.4:67653376-67653395 None:intergenic 30.0%
!!! AGTTGGTTTGTTTTCTAGTA+TGG - chr5.4:67653916-67653935 None:intergenic 30.0%
AAAGTCCTACACAAATTTCG+TGG - chr5.4:67653664-67653683 None:intergenic 35.0%
AAATAAAGCCGCAGATTACA+AGG + chr5.4:67654855-67654874 MS.gene070509:CDS 35.0%
AACCAACTTGGATCCATTTA+AGG + chr5.4:67653928-67653947 MS.gene070509:CDS 35.0%
AAGTGATGCTAGAACAAGAA+AGG - chr5.4:67655397-67655416 None:intergenic 35.0%
AATGTTTGCAGCATCTTACA+TGG + chr5.4:67654521-67654540 MS.gene070509:intron 35.0%
ACCAACTTGGATCCATTTAA+GGG + chr5.4:67653929-67653948 MS.gene070509:CDS 35.0%
ACTTTCTTAACTAGTGTTGC+TGG + chr5.4:67653524-67653543 MS.gene070509:intron 35.0%
AGGAAACACTATGTTACATG+AGG + chr5.4:67653023-67653042 MS.gene070509:CDS 35.0%
AGTGATGCTAGAACAAGAAA+GGG - chr5.4:67655396-67655415 None:intergenic 35.0%
AGTTACCACGAAATTTGTGT+AGG + chr5.4:67653656-67653675 MS.gene070509:intron 35.0%
CAAACAAAATGGAACAGAAC+AGG + chr5.4:67654678-67654697 MS.gene070509:CDS 35.0%
CAAATGCTGCACAATTCAAA+GGG + chr5.4:67654031-67654050 MS.gene070509:CDS 35.0%
CACAACCACAACAAAAATCA+CGG - chr5.4:67655301-67655320 None:intergenic 35.0%
CACGAAGAATCCAAACAAAA+TGG + chr5.4:67654667-67654686 MS.gene070509:CDS 35.0%
CCAAAAAGTGGATCTTGATA+TGG + chr5.4:67654349-67654368 MS.gene070509:CDS 35.0%
CCAAAAGATGCTAGATGAAT+TGG - chr5.4:67654074-67654093 None:intergenic 35.0%
CCTTGTTCACAAAACATAAC+AGG - chr5.4:67652993-67653012 None:intergenic 35.0%
CTCTACCCTATGTTTATATG+TGG - chr5.4:67654944-67654963 None:intergenic 35.0%
CTGTTATCAGTGAACTTACT+TGG + chr5.4:67655250-67655269 MS.gene070509:CDS 35.0%
CTTGAAACATTGCATGACAT+AGG - chr5.4:67655056-67655075 None:intergenic 35.0%
GCATCTATAGGTTACCTTAA+AGG + chr5.4:67653984-67654003 MS.gene070509:CDS 35.0%
GGATGCAGTCAAATTAATCT+TGG + chr5.4:67653206-67653225 MS.gene070509:CDS 35.0%
TACAAAACTACAGATCGGTT+CGG + chr5.4:67653087-67653106 MS.gene070509:CDS 35.0%
TACTGATGCTGTCACTATAA+GGG - chr5.4:67654986-67655005 None:intergenic 35.0%
TATCAGTGAACTTACTTGGT+TGG + chr5.4:67655254-67655273 MS.gene070509:CDS 35.0%
TTTACCTTGCGGTTGAAAAT+GGG + chr5.4:67653178-67653197 MS.gene070509:CDS 35.0%
! AAAATTGTAATGCCGCTTCT+TGG + chr5.4:67655180-67655199 MS.gene070509:CDS 35.0%
! ATTCATCTAGCATCTTTTGG+TGG + chr5.4:67654074-67654093 MS.gene070509:CDS 35.0%
! CACTGTACCAAATCTCTTTT+TGG + chr5.4:67654903-67654922 MS.gene070509:CDS 35.0%
! CCAATTCATCTAGCATCTTT+TGG + chr5.4:67654071-67654090 MS.gene070509:CDS 35.0%
! CCTGTTATGTTTTGTGAACA+AGG + chr5.4:67652990-67653009 MS.gene070509:CDS 35.0%
! CTGTTCTGTTCCATTTTGTT+TGG - chr5.4:67654680-67654699 None:intergenic 35.0%
! GTATCACATCATGTTTTCCA+TGG - chr5.4:67654199-67654218 None:intergenic 35.0%
! TGCTCTCATCTCACTATTTT+TGG + chr5.4:67655206-67655225 MS.gene070509:CDS 35.0%
! TGTTACCGTGATTTTTGTTG+TGG + chr5.4:67655293-67655312 MS.gene070509:CDS 35.0%
!! CATTTTGTTTGGATTCTTCG+TGG - chr5.4:67654669-67654688 None:intergenic 35.0%
!! CCATATCAAGATCCACTTTT+TGG - chr5.4:67654352-67654371 None:intergenic 35.0%
!! GAACTTTGAAGTTGATGATG+AGG - chr5.4:67655352-67655371 None:intergenic 35.0%
!! TATCTGAGGCTTTTGCTTTT+TGG - chr5.4:67654765-67654784 None:intergenic 35.0%
!!! CTCACTATTTTTGGCTTTCA+TGG + chr5.4:67655215-67655234 MS.gene070509:CDS 35.0%
AAAGCAAAGTCCAAGAAGAG+TGG + chr5.4:67654567-67654586 MS.gene070509:CDS 40.0%
AACTAAGTAGCACACAGTTG+TGG - chr5.4:67654327-67654346 None:intergenic 40.0%
ACTAAGTAGCACACAGTTGT+GGG - chr5.4:67654326-67654345 None:intergenic 40.0%
ATAGATGCTGCGTAATGAAG+TGG - chr5.4:67653972-67653991 None:intergenic 40.0%
ATCTTGCAGCCAAATGCAAA+GGG - chr5.4:67654295-67654314 None:intergenic 40.0%
CAAGAGCCGTTTCATTATTG+TGG - chr5.4:67654379-67654398 None:intergenic 40.0%
CTACTGATGCTGTCACTATA+AGG - chr5.4:67654987-67655006 None:intergenic 40.0%
CTTCATGATAGCTGCTACAA+CGG - chr5.4:67653274-67653293 None:intergenic 40.0%
CTTCATTACGCAGCATCTAT+AGG + chr5.4:67653972-67653991 MS.gene070509:CDS 40.0%
CTTTACCTTGCGGTTGAAAA+TGG + chr5.4:67653177-67653196 MS.gene070509:CDS 40.0%
GAAATCGGTACTTTACCTTG+CGG + chr5.4:67653167-67653186 MS.gene070509:CDS 40.0%
GCAAATGCTGCACAATTCAA+AGG + chr5.4:67654030-67654049 MS.gene070509:CDS 40.0%
GCTATCATGAAGCAGAATCA+AGG + chr5.4:67653282-67653301 MS.gene070509:CDS 40.0%
GTGATGCTAGAACAAGAAAG+GGG - chr5.4:67655395-67655414 None:intergenic 40.0%
TAGAACAAGAAAGGGGTAGT+AGG - chr5.4:67655388-67655407 None:intergenic 40.0%
TAGGGTAGAGACACTGATAT+TGG + chr5.4:67654954-67654973 MS.gene070509:CDS 40.0%
TCCCTTAAATGGATCCAAGT+TGG - chr5.4:67653933-67653952 None:intergenic 40.0%
TGTTGTGTCCTTGTAATCTG+CGG - chr5.4:67654866-67654885 None:intergenic 40.0%
TTACCTTGCGGTTGAAAATG+GGG + chr5.4:67653179-67653198 MS.gene070509:CDS 40.0%
TTCATGATAGCTGCTACAAC+GGG - chr5.4:67653273-67653292 None:intergenic 40.0%
TTCTAGCATCACTTGCAGAA+GGG + chr5.4:67655403-67655422 MS.gene070509:CDS 40.0%
! AAAGTGTCAAGCATCTCTGT+GGG - chr5.4:67654143-67654162 None:intergenic 40.0%
! ACAAAACTACAGATCGGTTC+GGG + chr5.4:67653088-67653107 MS.gene070509:CDS 40.0%
! AGATTTGGTACAGTGTTCTC+GGG - chr5.4:67654898-67654917 None:intergenic 40.0%
! GAGATGCTTGACACTTTTCT+TGG + chr5.4:67654146-67654165 MS.gene070509:CDS 40.0%
! GAGATTTGGTACAGTGTTCT+CGG - chr5.4:67654899-67654918 None:intergenic 40.0%
! TGCTACTATCCTCCTCTTTT+GGG + chr5.4:67655119-67655138 MS.gene070509:CDS 40.0%
!! AAAAGTGTCAAGCATCTCTG+TGG - chr5.4:67654144-67654163 None:intergenic 40.0%
!! TTGAAGTTGATGATGAGGGA+AGG - chr5.4:67655347-67655366 None:intergenic 40.0%
!! TTGATGATGAGGGAAGGAAA+AGG - chr5.4:67655341-67655360 None:intergenic 40.0%
!!! CTATTTTTGGCTTTCATGGC+TGG + chr5.4:67655219-67655238 MS.gene070509:CDS 40.0%
ATGAGAGCAGTTCCAAGAAG+CGG - chr5.4:67655195-67655214 None:intergenic 45.0%
CCCTGAGAATGATGATAAGC+CGG + chr5.4:67653236-67653255 MS.gene070509:CDS 45.0%
CCGGCTTATCATCATTCTCA+GGG - chr5.4:67653239-67653258 None:intergenic 45.0%
CCTGTCGCCAAAAAGAGATT+TGG - chr5.4:67654913-67654932 None:intergenic 45.0%
CTTCACATTGCAGCAAACCA+TGG + chr5.4:67654179-67654198 MS.gene070509:CDS 45.0%
GAGAATGATGATAAGCCGGA+GGG + chr5.4:67653240-67653259 MS.gene070509:CDS 45.0%
GAGGAGGATAGTAGCACTAA+TGG - chr5.4:67655115-67655134 None:intergenic 45.0%
GATCTTGCAGCCAAATGCAA+AGG - chr5.4:67654296-67654315 None:intergenic 45.0%
GGTAACAGCCAGAAAGAGAT+TGG - chr5.4:67655280-67655299 None:intergenic 45.0%
GTGAACAAGGAGAATGCTCA+AGG + chr5.4:67653003-67653022 MS.gene070509:CDS 45.0%
GTTCTAGCATCACTTGCAGA+AGG + chr5.4:67655402-67655421 MS.gene070509:CDS 45.0%
TCCGGCTTATCATCATTCTC+AGG - chr5.4:67653240-67653259 None:intergenic 45.0%
TCTTGCAGCCAAATGCAAAG+GGG - chr5.4:67654294-67654313 None:intergenic 45.0%
TGAGAATGATGATAAGCCGG+AGG + chr5.4:67653239-67653258 MS.gene070509:CDS 45.0%
TGATGAGGGAAGGAAAAGGA+CGG - chr5.4:67655337-67655356 None:intergenic 45.0%
! ACTTTTCCTTCTGCTTCACC+TGG - chr5.4:67655027-67655046 None:intergenic 45.0%
! ATATGGAGTGCCACTCTTCT+TGG - chr5.4:67654580-67654599 None:intergenic 45.0%
! ATCCAAGTGTTGCCCAAAAG+AGG - chr5.4:67655134-67655153 None:intergenic 45.0%
! TTGGTTGGCCAATCTCTTTC+TGG + chr5.4:67655269-67655288 MS.gene070509:CDS 45.0%
!! CCAAATCTCTTTTTGGCGAC+AGG + chr5.4:67654910-67654929 MS.gene070509:CDS 45.0%
!! GTGCTACTATCCTCCTCTTT+TGG + chr5.4:67655118-67655137 MS.gene070509:CDS 45.0%
!!! GCTTTTGCTTTTTGGCACTG+CGG - chr5.4:67654757-67654776 None:intergenic 45.0%
GCAGCATGTTTAGCTGTTCC+AGG + chr5.4:67655006-67655025 MS.gene070509:CDS 50.0%
TCAAGCATCTCTGTGGGATC+AGG - chr5.4:67654137-67654156 None:intergenic 50.0%
TGCGGTTGAAAATGGGGACA+AGG + chr5.4:67653185-67653204 MS.gene070509:CDS 50.0%
TGGACTGCACTGAAATCTGC+TGG + chr5.4:67654541-67654560 MS.gene070509:CDS 50.0%
TGGAGACACCCCTTTGCATT+TGG + chr5.4:67654283-67654302 MS.gene070509:CDS 50.0%
! CAAGTGTTGCCCAAAAGAGG+AGG - chr5.4:67655131-67655150 None:intergenic 50.0%
! CTCCTCTTTTGGGCAACACT+TGG + chr5.4:67655129-67655148 MS.gene070509:CDS 50.0%
! TGTCCCCATTTTCAACCGCA+AGG - chr5.4:67653185-67653204 None:intergenic 50.0%
ACAACGGGTGATAGTCCCTC+CGG - chr5.4:67653258-67653277 None:intergenic 55.0%
GCTGTTCCAGGTGAAGCAGA+AGG + chr5.4:67655018-67655037 MS.gene070509:CDS 55.0%
!! AGCATCTTTTGGTGGCCACG+TGG + chr5.4:67654082-67654101 MS.gene070509:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 67652982 67655428 67652982 ID=MS.gene070509
chr5.4 mRNA 67652982 67655428 67652982 ID=MS.gene070509.t1;Parent=MS.gene070509
chr5.4 exon 67652982 67653303 67652982 ID=MS.gene070509.t1.exon1;Parent=MS.gene070509.t1
chr5.4 CDS 67652982 67653303 67652982 ID=cds.MS.gene070509.t1;Parent=MS.gene070509.t1
chr5.4 exon 67653901 67654463 67653901 ID=MS.gene070509.t1.exon2;Parent=MS.gene070509.t1
chr5.4 CDS 67653901 67654463 67653901 ID=cds.MS.gene070509.t1;Parent=MS.gene070509.t1
chr5.4 exon 67654532 67655428 67654532 ID=MS.gene070509.t1.exon3;Parent=MS.gene070509.t1
chr5.4 CDS 67654532 67655428 67654532 ID=cds.MS.gene070509.t1;Parent=MS.gene070509.t1
Gene Sequence

>MS.gene070509

ATGGAGGACCTGTTATGTTTTGTGAACAAGGAGAATGCTCAAGGAAACACTATGTTACATGAGGCAATATTATATGGGGATAAAGTCTTCAAAGTTTTTGAACGTTACAAAACTACAGATCGGTTCGGGAACTCATTGTCAAATTGTTGTTATGAACTTGCGTTAGCTTTTGTTAATCATGCAAAGAAATCGGTACTTTACCTTGCGGTTGAAAATGGGGACAAGGATGCAGTCAAATTAATCTTGGAGAATTGCCCTGAGAATGATGATAAGCCGGAGGGACTATCACCCGTTGTAGCAGCTATCATGAAGCAGAATCAAGGTATGTAATTAAATAGTTAAGTTGATCATTATATTGTTTTTCAATCACTTGAATGATAACACTATGTTTCCTTTGTCTAAAAAACACCCTTTTTGTTTATTAAATGGGTTTCACCAATAGTTTATGTCAATACATTTTTACAATATTCATCTATTTAGCTTAAAACTAAATAGATAAAGTATCCACCATCGTTGTTTTCAATAAATAAAAATTATTTCTAACTTTCTTAACTAGTGTTGCTGGACAATAGTTAACATTTTCCTTTATTTATTTTCTATCTTAGCTAATGTAAGTCTTACTCGAAAACACATCTAAGTATTTAACATTGTTTCTTAATTAGTTGAAATTTACAAGTTACCACGAAATTTGTGTAGGACTTTTTTTGAGAGCTAATTTGTGTAGGAATTATTTTCAAAATAGTGAGATGTATTTAAATATCAACCGAAGGAAGATATATAGAATATTTTGACAAATGTTGCTAGCCCTAGTTCTTTGTAATATTAGTCATTTCTCAAATAATATCCGAAAATAACAAATGTTGCTAGCTGGAGTATGAGTTAATTACATTATTGGATAATAATTATGTTTATGTGTCAGAAATGCTCAGCACCATACTAGAAAACAAACCAACTTGGATCCATTTAAGGGACAAAGATGAAAGGCTTCCACTTCATTACGCAGCATCTATAGGTTACCTTAAAGGTGTTGAATTATTACTTGACGAATGCAAATGCTGCACAATTCAAAGGGACAAATATGGTTATTTCCCAATTCATCTAGCATCTTTTGGTGGCCACGTGGAAATAGTAAAAAAATTGTTGGAATACTGTCCTGATCCCACAGAGATGCTTGACACTTTTCTTGGACGTAATTTTCTTCACATTGCAGCAAACCATGGAAAACATGATGTGATACGTTACATACTTGAAAGTGAAGAAGTTCGTGAACGCCGTCAGATGATAAATCAGAAAGATAGGAATGGAGACACCCCTTTGCATTTGGCTGCAAGATCATGTCATCCCACAACTGTGTGCTACTTAGTTAACCAAAAAGTGGATCTTGATATGGTTAACCACAATAATGAAACGGCTCTTGACATTGTTAATTCACTGTACGAGAGTAATAAATCATCTTTAAGACAGGCAAGTAGTTCTTGCATAGTTATTATCATATTTACGAGCTTCATTTCTTCTTTCATAAAGAGAAACTAATGTAACAATGTTTGCAGCATCTTACATGGACTGCACTGAAATCTGCTGGTGCAAAGCAAAGTCCAAGAAGAGTGGCACTCCATATTGAATCCAAATCAAATGATTCAAATTCACCAGAAAAAATAGAACAAGAGAAAGACAAAAAACAAGACCACGAAGAATCCAAACAAAATGGAACAGAACAGGACAATGAAAGATCAAACATAACAATGACAGAAGAAAAGCGAACAAGAAAAACACCGCAGTGCCAAAAAGCAAAAGCCTCAGATATTTACATGACAACAACAGAAAAAAAGAAAACAGGAAAAGAACAAGAAAAAGATCAAGTTAAAGTAAAAGGAAATAAAGCCGCAGATTACAAGGACACAACAGAAAAAGTTCCCGAGAACACTGTACCAAATCTCTTTTTGGCGACAGGCCCTAACACCACATATAAACATAGGGTAGAGACACTGATATTGGTTTCAACCCTTATAGTGACAGCATCAGTAGCAGCATGTTTAGCTGTTCCAGGTGAAGCAGAAGGAAAAGTAAACAACCTATGTCATGCAATGTTTCAAGCTTTCATTATCTTCATCACAATATCATTGTTCAGTTCCATTAGTGCTACTATCCTCCTCTTTTGGGCAACACTTGGATTAACTGAATTGGCGACTTTCACTCTGAAAATTGTAATGCCGCTTCTTGGAACTGCTCTCATCTCACTATTTTTGGCTTTCATGGCTGGTCTCTACACTGTTATCAGTGAACTTACTTGGTTGGCCAATCTCTTTCTGGCTGTTACCGTGATTTTTGTTGTGGTTGTGTTTCTGTTGTACACCGTCCTTTTCCTTCCCTCATCATCAACTTCAAAGTTCTTTCGATACATTTCCTACTACCCCTTTCTTGTTCTAGCATCACTTGCAGAAGGGTAA
Protein sequence

>MS.gene070509.t1

MEDLLCFVNKENAQGNTMLHEAILYGDKVFKVFERYKTTDRFGNSLSNCCYELALAFVNHAKKSVLYLAVENGDKDAVKLILENCPENDDKPEGLSPVVAAIMKQNQEMLSTILENKPTWIHLRDKDERLPLHYAASIGYLKGVELLLDECKCCTIQRDKYGYFPIHLASFGGHVEIVKKLLEYCPDPTEMLDTFLGRNFLHIAANHGKHDVIRYILESEEVRERRQMINQKDRNGDTPLHLAARSCHPTTVCYLVNQKVDLDMVNHNNETALDIVNSLYESNKSSLRQASSSCIHLTWTALKSAGAKQSPRRVALHIESKSNDSNSPEKIEQEKDKKQDHEESKQNGTEQDNERSNITMTEEKRTRKTPQCQKAKASDIYMTTTEKKKTGKEQEKDQVKVKGNKAADYKDTTEKVPENTVPNLFLATGPNTTYKHRVETLILVSTLIVTASVAACLAVPGEAEGKVNNLCHAMFQAFIIFITISLFSSISATILLFWATLGLTELATFTLKIVMPLLGTALISLFLAFMAGLYTVISELTWLANLFLAVTVIFVVVVFLLYTVLFLPSSSTSKFFRYISYYPFLVLASLAEG