Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65673.t1 | XP_003614610.2 | 95 | 282 | 13 | 1 | 1 | 281 | 15 | 296 | 2.70E-156 | 561.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65673.t1 | Q9C9G4 | 62.1 | 269 | 96 | 5 | 6 | 271 | 24 | 289 | 1.8e-92 | 340.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65673.t1 | G7K0J9 | 95.0 | 282 | 13 | 1 | 1 | 281 | 15 | 296 | 1.9e-156 | 561.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene65673 | 0.830917 | 2.24E-55 | -1.69E-46 |
| MS.gene049567 | MS.gene65673 | 0.816617 | 5.04E-52 | -1.69E-46 |
| MS.gene049825 | MS.gene65673 | 0.801883 | 7.30E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene65673 | 0.80778 | 4.28E-50 | -1.69E-46 |
| MS.gene052333 | MS.gene65673 | 0.816505 | 5.34E-52 | -1.69E-46 |
| MS.gene052582 | MS.gene65673 | 0.809289 | 2.04E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene65673 | 0.815395 | 9.45E-52 | -1.69E-46 |
| MS.gene052586 | MS.gene65673 | 0.81626 | 6.06E-52 | -1.69E-46 |
| MS.gene052685 | MS.gene65673 | 0.815401 | 9.42E-52 | -1.69E-46 |
| MS.gene052768 | MS.gene65673 | 0.800879 | 1.17E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene65673 | 0.807581 | 4.71E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene65673 | 0.803781 | 2.96E-49 | -1.69E-46 |
| MS.gene05414 | MS.gene65673 | 0.802057 | 6.72E-49 | -1.69E-46 |
| MS.gene054222 | MS.gene65673 | 0.809737 | 1.63E-50 | -1.69E-46 |
| MS.gene05423 | MS.gene65673 | 0.815705 | 8.06E-52 | -1.69E-46 |
| MS.gene054291 | MS.gene65673 | -0.821713 | 3.49E-53 | -1.69E-46 |
| MS.gene054576 | MS.gene65673 | 0.817023 | 4.09E-52 | -1.69E-46 |
| MS.gene056050 | MS.gene65673 | 0.810007 | 1.43E-50 | -1.69E-46 |
| MS.gene056779 | MS.gene65673 | 0.822219 | 2.66E-53 | -1.69E-46 |
| MS.gene05686 | MS.gene65673 | 0.8009 | 1.16E-48 | -1.69E-46 |
| MS.gene058625 | MS.gene65673 | 0.809881 | 1.52E-50 | -1.69E-46 |
| MS.gene059272 | MS.gene65673 | 0.807953 | 3.93E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene65673 | 0.805653 | 1.20E-49 | -1.69E-46 |
| MS.gene060840 | MS.gene65673 | 0.806115 | 9.62E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene047520 | MS.gene65673 | PPI |
| MS.gene65673 | MS.gene052539 | PPI |
| MS.gene65673 | MS.gene33739 | PPI |
| MS.gene32189 | MS.gene65673 | PPI |
| MS.gene65673 | MS.gene01769 | PPI |
| MS.gene035220 | MS.gene65673 | PPI |
| MS.gene65673 | MS.gene002383 | PPI |
| MS.gene65673 | MS.gene01361 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65673.t1 | MTR_5g056160 | 95.035 | 282 | 13 | 1 | 1 | 281 | 15 | 296 | 0.0 | 561 |
| MS.gene65673.t1 | MTR_5g056140 | 79.078 | 282 | 56 | 2 | 1 | 281 | 15 | 294 | 1.60e-168 | 468 |
| MS.gene65673.t1 | MTR_1g110510 | 69.118 | 272 | 80 | 3 | 1 | 271 | 15 | 283 | 2.76e-137 | 389 |
| MS.gene65673.t1 | MTR_4g090610 | 57.143 | 280 | 114 | 4 | 6 | 280 | 16 | 294 | 8.24e-110 | 320 |
| MS.gene65673.t1 | MTR_4g090610 | 57.143 | 280 | 114 | 4 | 6 | 280 | 60 | 338 | 1.35e-109 | 320 |
| MS.gene65673.t1 | MTR_4g090610 | 57.143 | 280 | 114 | 4 | 6 | 280 | 105 | 383 | 2.17e-109 | 322 |
| MS.gene65673.t1 | MTR_1g110510 | 67.580 | 219 | 67 | 3 | 1 | 218 | 15 | 230 | 1.87e-106 | 308 |
| MS.gene65673.t1 | MTR_2g012440 | 45.652 | 276 | 138 | 6 | 3 | 273 | 25 | 293 | 8.49e-81 | 246 |
| MS.gene65673.t1 | MTR_4g090610 | 56.122 | 196 | 80 | 4 | 6 | 196 | 16 | 210 | 5.19e-70 | 217 |
| MS.gene65673.t1 | MTR_4g090610 | 56.122 | 196 | 80 | 4 | 6 | 196 | 60 | 254 | 7.74e-70 | 218 |
| MS.gene65673.t1 | MTR_5g056560 | 75.200 | 125 | 31 | 0 | 117 | 241 | 35 | 159 | 4.14e-67 | 207 |
| MS.gene65673.t1 | MTR_2g012440 | 46.190 | 210 | 102 | 5 | 3 | 207 | 25 | 228 | 2.08e-61 | 194 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65673.t1 | AT1G68290 | 62.082 | 269 | 96 | 5 | 6 | 271 | 24 | 289 | 1.19e-117 | 339 |
| MS.gene65673.t1 | AT4G21585 | 54.770 | 283 | 122 | 4 | 3 | 280 | 18 | 299 | 2.99e-104 | 305 |
| MS.gene65673.t1 | AT4G21585 | 54.770 | 283 | 122 | 4 | 3 | 280 | 18 | 299 | 2.99e-104 | 305 |
| MS.gene65673.t1 | AT4G21590 | 53.737 | 281 | 119 | 4 | 3 | 278 | 18 | 292 | 4.04e-100 | 295 |
| MS.gene65673.t1 | AT4G21590 | 53.737 | 281 | 119 | 4 | 3 | 278 | 18 | 292 | 4.04e-100 | 295 |
| MS.gene65673.t1 | AT4G21585 | 49.838 | 309 | 123 | 4 | 3 | 280 | 18 | 325 | 4.22e-99 | 293 |
| MS.gene65673.t1 | AT4G21585 | 49.839 | 311 | 122 | 5 | 3 | 280 | 18 | 327 | 4.07e-98 | 291 |
| MS.gene65673.t1 | AT4G21600 | 50.709 | 282 | 132 | 5 | 3 | 278 | 14 | 294 | 5.92e-94 | 279 |
| MS.gene65673.t1 | AT1G11190 | 47.857 | 280 | 139 | 6 | 4 | 278 | 23 | 300 | 7.74e-91 | 272 |
| MS.gene65673.t1 | AT4G21585 | 45.306 | 245 | 102 | 4 | 3 | 216 | 18 | 261 | 2.58e-68 | 213 |
| MS.gene65673.t1 | AT4G21585 | 45.306 | 245 | 102 | 4 | 3 | 216 | 18 | 261 | 2.58e-68 | 213 |
Find 51 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAATTTCATTTATGCTTT+TGG | 0.199134 | 5.2:-48283518 | None:intergenic |
| AAATCATTGTTTGCAGATTC+TGG | 0.203012 | 5.2:+48283237 | None:intergenic |
| GTAGCGTAGACGCGTGTAAA+TGG | 0.226011 | 5.2:-48263457 | MS.gene65673:CDS |
| TCTCCTGCAAACAGAAAGTA+TGG | 0.250152 | 5.2:-48263901 | MS.gene65673:intron |
| AGATCAGAAAACTGGAATTA+AGG | 0.262022 | 5.2:-48264826 | MS.gene65673:CDS |
| ACCAACCAGCTCCTCGATTA+TGG | 0.300284 | 5.2:-48264771 | MS.gene65673:CDS |
| GATCAGAAAACTGGAATTAA+GGG | 0.317310 | 5.2:-48264825 | MS.gene65673:CDS |
| TAAAATGTGAAAGGAAATAA+AGG | 0.321153 | 5.2:+48264590 | None:intergenic |
| ATTTATCTAGCAAGTGTTCA+TGG | 0.394091 | 5.2:-48283218 | MS.gene65673:CDS |
| GATTGTAAAGATCAGAAAAC+TGG | 0.404647 | 5.2:-48264834 | MS.gene65673:CDS |
| TGGTTCAAAATACAAAAGAA+TGG | 0.418318 | 5.2:-48283498 | MS.gene65673:CDS |
| GGTTCAAAATACAAAAGAAT+GGG | 0.420611 | 5.2:-48283497 | MS.gene65673:CDS |
| TAGCGTAGACGCGTGTAAAT+GGG | 0.423596 | 5.2:-48263456 | MS.gene65673:CDS |
| TTGTAATCGCAAACATAGTC+TGG | 0.425321 | 5.2:+48283138 | None:intergenic |
| ACAAAAGAATGGGGAGAAGA+TGG | 0.426520 | 5.2:-48283487 | MS.gene65673:CDS |
| GGAAATAAAGGGCTTGTGTA+AGG | 0.450918 | 5.2:+48264602 | None:intergenic |
| GAGAATGGAACATACATTGA+TGG | 0.462474 | 5.2:+48263827 | None:intergenic |
| TGCAATTCAAGAAAATATTA+CGG | 0.473579 | 5.2:-48264016 | MS.gene65673:intron |
| CATTGTTTGTAAGATTGCTC+AGG | 0.497846 | 5.2:-48283458 | MS.gene65673:intron |
| ATGTGAGGTTTATCTATCCT+TGG | 0.499257 | 5.2:-48283188 | MS.gene65673:CDS |
| GGGCTAAAGAAGTCGAAGAA+TGG | 0.502003 | 5.2:-48263880 | MS.gene65673:CDS |
| CACTGCCATAATCGAGGAGC+TGG | 0.502304 | 5.2:+48264766 | None:intergenic |
| GCAATGAAATAACTGTTCAT+TGG | 0.502502 | 5.2:-48264431 | MS.gene65673:CDS |
| ATTAAGGGGCGATGTGTTGT+GGG | 0.504054 | 5.2:-48264810 | MS.gene65673:CDS |
| GGCTAAAGAAGTCGAAGAAT+GGG | 0.505630 | 5.2:-48263879 | MS.gene65673:CDS |
| AAAATGTGAAAGGAAATAAA+GGG | 0.515292 | 5.2:+48264591 | None:intergenic |
| AATTAAGGGGCGATGTGTTG+TGG | 0.528844 | 5.2:-48264811 | MS.gene65673:CDS |
| TGTGGCTTTGTCTCAGACAA+AGG | 0.532341 | 5.2:-48264456 | MS.gene65673:CDS |
| AGCTCAAGCAGGAATTCGAT+TGG | 0.533221 | 5.2:-48263263 | MS.gene65673:CDS |
| TAACGATAGGAAAACGTGAC+AGG | 0.535783 | 5.2:+48263296 | None:intergenic |
| ACTTAGCGAGGCAGCTGCAA+AGG | 0.541239 | 5.2:-48283277 | MS.gene65673:CDS |
| CAAAAGAATGGGGAGAAGAT+GGG | 0.546873 | 5.2:-48283486 | MS.gene65673:CDS |
| AATTTGCGATTAGCTCAAGC+AGG | 0.557472 | 5.2:-48263274 | MS.gene65673:CDS |
| TCTGAAGGCTCAACACTCAA+AGG | 0.561237 | 5.2:-48263419 | MS.gene65673:intron |
| TTTATCTAGCAAGTGTTCAT+GGG | 0.562069 | 5.2:-48283217 | MS.gene65673:CDS |
| AGAATGGAACATACATTGAT+GGG | 0.569505 | 5.2:+48263828 | None:intergenic |
| AGATTCTATGACTTAGAGCT+AGG | 0.570104 | 5.2:-48264051 | MS.gene65673:CDS |
| GCCATAATCGAGGAGCTGGT+TGG | 0.571467 | 5.2:+48264770 | None:intergenic |
| TGTCTCCCATAAAATGTGAA+AGG | 0.584972 | 5.2:+48264581 | None:intergenic |
| GCTAATCGCAAATTAACGAT+AGG | 0.590416 | 5.2:+48263283 | None:intergenic |
| TATCTGCAGCCTCTGCATTG+TGG | 0.594743 | 5.2:-48264474 | MS.gene65673:intron |
| GTTCAAAATACAAAAGAATG+GGG | 0.613268 | 5.2:-48283496 | MS.gene65673:CDS |
| AGAGCAGGCTCGACTTAGCG+AGG | 0.618317 | 5.2:-48283289 | MS.gene65673:intron |
| GTTCATGGGCAGATCATGTG+AGG | 0.627592 | 5.2:-48283203 | MS.gene65673:CDS |
| GCATATAAAGATGCATCTGA+AGG | 0.632248 | 5.2:-48263434 | MS.gene65673:CDS |
| ATCAGAAAACTGGAATTAAG+GGG | 0.638877 | 5.2:-48264824 | MS.gene65673:CDS |
| CTCCTGCAAACAGAAAGTAT+GGG | 0.644964 | 5.2:-48263900 | MS.gene65673:intron |
| TTATGGCACTGCCATAATCG+AGG | 0.653840 | 5.2:+48264760 | None:intergenic |
| GAGACAAAGCCACAATGCAG+AGG | 0.656818 | 5.2:+48264465 | None:intergenic |
| GGCTTTGTCTCAGACAAAGG+AGG | 0.657039 | 5.2:-48264453 | MS.gene65673:CDS |
| AAGTGCAAAGCAGACGACCA+AGG | 0.658164 | 5.2:+48283171 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 48263207 | 48283527 | 48263207 | ID=MS.gene65673 |
| chr5.2 | mRNA | 48263207 | 48283527 | 48263207 | ID=MS.gene65673.t1;Parent=MS.gene65673 |
| chr5.2 | exon | 48283459 | 48283527 | 48283459 | ID=MS.gene65673.t1.exon1;Parent=MS.gene65673.t1 |
| chr5.2 | CDS | 48283459 | 48283527 | 48283459 | ID=cds.MS.gene65673.t1;Parent=MS.gene65673.t1 |
| chr5.2 | exon | 48283133 | 48283304 | 48283133 | ID=MS.gene65673.t1.exon2;Parent=MS.gene65673.t1 |
| chr5.2 | CDS | 48283133 | 48283304 | 48283133 | ID=cds.MS.gene65673.t1;Parent=MS.gene65673.t1 |
| chr5.2 | exon | 48264751 | 48264858 | 48264751 | ID=MS.gene65673.t1.exon3;Parent=MS.gene65673.t1 |
| chr5.2 | CDS | 48264751 | 48264858 | 48264751 | ID=cds.MS.gene65673.t1;Parent=MS.gene65673.t1 |
| chr5.2 | exon | 48264573 | 48264628 | 48264573 | ID=MS.gene65673.t1.exon4;Parent=MS.gene65673.t1 |
| chr5.2 | CDS | 48264573 | 48264628 | 48264573 | ID=cds.MS.gene65673.t1;Parent=MS.gene65673.t1 |
| chr5.2 | exon | 48264404 | 48264487 | 48264404 | ID=MS.gene65673.t1.exon5;Parent=MS.gene65673.t1 |
| chr5.2 | CDS | 48264404 | 48264487 | 48264404 | ID=cds.MS.gene65673.t1;Parent=MS.gene65673.t1 |
| chr5.2 | exon | 48264017 | 48264109 | 48264017 | ID=MS.gene65673.t1.exon6;Parent=MS.gene65673.t1 |
| chr5.2 | CDS | 48264017 | 48264109 | 48264017 | ID=cds.MS.gene65673.t1;Parent=MS.gene65673.t1 |
| chr5.2 | exon | 48263842 | 48263909 | 48263842 | ID=MS.gene65673.t1.exon7;Parent=MS.gene65673.t1 |
| chr5.2 | CDS | 48263842 | 48263909 | 48263842 | ID=cds.MS.gene65673.t1;Parent=MS.gene65673.t1 |
| chr5.2 | exon | 48263420 | 48263493 | 48263420 | ID=MS.gene65673.t1.exon8;Parent=MS.gene65673.t1 |
| chr5.2 | CDS | 48263420 | 48263493 | 48263420 | ID=cds.MS.gene65673.t1;Parent=MS.gene65673.t1 |
| chr5.2 | exon | 48263207 | 48263328 | 48263207 | ID=MS.gene65673.t1.exon9;Parent=MS.gene65673.t1 |
| chr5.2 | CDS | 48263207 | 48263328 | 48263207 | ID=cds.MS.gene65673.t1;Parent=MS.gene65673.t1 |
| Gene Sequence |
| Protein sequence |
