AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65949

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65949.t1 MTR_7g101050 80.944 593 51 8 1 548 1 576 0.0 937
MS.gene65949.t1 MTR_8g102050 83.877 552 65 3 19 548 2 551 0.0 930
MS.gene65949.t1 MTR_4g100920 49.724 543 216 10 12 548 55 546 2.12e-175 506
MS.gene65949.t1 MTR_8g011530 43.902 574 278 11 5 547 72 632 7.98e-156 459
MS.gene65949.t1 MTR_4g100920 45.558 439 182 10 12 444 55 442 1.25e-118 358
MS.gene65949.t1 MTR_1g014140 30.249 281 176 8 272 545 271 538 2.19e-26 113
MS.gene65949.t1 MTR_5g028360 29.893 281 177 9 272 545 319 586 2.58e-25 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65949.t1 AT5G56680 63.826 575 178 4 1 548 1 572 0.0 767
MS.gene65949.t1 AT1G70980 63.763 574 179 6 1 548 1 571 0.0 743
MS.gene65949.t1 AT4G17300 49.718 531 211 10 25 548 86 567 5.18e-173 501
MS.gene65949.t1 AT4G17300 49.718 531 211 10 25 548 106 587 6.70e-173 501
MS.gene65949.t1 AT3G07420 45.238 504 246 8 68 547 140 637 7.00e-148 439
MS.gene65949.t1 AT3G07420 42.037 383 192 8 68 426 140 516 1.90e-92 293
MS.gene65949.t1 AT1G68420 63.636 77 28 0 302 378 1 77 1.24e-28 109
MS.gene65949.t1 AT4G31180 32.384 281 170 9 272 545 288 555 1.85e-28 119
MS.gene65949.t1 AT4G31180 32.384 281 170 9 272 545 288 555 1.85e-28 119
MS.gene65949.t1 AT4G26870 30.769 286 160 10 272 541 262 525 4.33e-27 115
MS.gene65949.t1 AT5G38750 34.146 164 65 4 18 178 4 127 1.11e-16 77.4

Find 137 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAATATCCTTTGCCTAAA+AGG 0.177868 1.1:+27044358 MS.gene65949:CDS
CCAGGCATTTGGCTGAATTT+TGG 0.190908 1.1:+27044904 MS.gene65949:CDS
TCTTGAGGATATGCAATTTA+TGG 0.198036 1.1:+27045025 MS.gene65949:CDS
CTTAGACAACTTGTTCTTAC+TGG 0.233716 1.1:+27044222 MS.gene65949:CDS
TAGAATGGTTTGATACCTTT+TGG 0.274490 1.1:-27045269 None:intergenic
TTGCATCGATCGTTTGAAAA+TGG 0.283409 1.1:+27045067 MS.gene65949:CDS
AGGAATATCGGGCTGAAGTT+TGG 0.310492 1.1:-27044727 None:intergenic
AATTTGAGAAGGAAGTAAAA+TGG 0.311241 1.1:+27045168 MS.gene65949:CDS
CATCTCAAACTTCCTCATTC+TGG 0.318006 1.1:+27044267 MS.gene65949:CDS
TTCTCACACTTCCAGGCATT+TGG 0.323004 1.1:+27044893 MS.gene65949:CDS
TACCATTCATATGGCTTAAC+AGG 0.325352 1.1:-27045428 None:intergenic
GCCGTCTTCACGGCAGATTA+TGG 0.326140 1.1:-27044049 None:intergenic
TCCGTCCTTTCGAGGAATAT+CGG 0.331192 1.1:-27044739 None:intergenic
AAGTCTGCAAACGCGATTTC+AGG 0.337547 1.1:-27044936 None:intergenic
AACGATAACTTGAAGGTTAC+CGG 0.339980 1.1:-27044181 None:intergenic
CTTAGTAGTGTGTATACATT+TGG 0.341074 1.1:+27044849 MS.gene65949:CDS
TCTTATACTTCGTCTCTGTT+AGG 0.342134 1.1:-27045223 None:intergenic
ATGGCTGATAAGGTTGATAA+AGG 0.344858 1.1:+27045044 MS.gene65949:CDS
ATTTGAGAAGGAAGTAAAAT+GGG 0.355264 1.1:+27045169 MS.gene65949:CDS
CCAAAGGTTGGAGAGTTAAT+TGG 0.360690 1.1:+27045344 MS.gene65949:CDS
CAGAGAATTCTCACACTTCC+AGG 0.362661 1.1:+27044886 MS.gene65949:CDS
TACAGAAGCCGTGGAAATAC+TGG 0.367856 1.1:+27045121 MS.gene65949:CDS
AGCAGATATCGTGTTGGTTC+TGG 0.374309 1.1:-27044424 None:intergenic
TGAAATCGCGTTTGCAGACT+TGG 0.375982 1.1:+27044938 MS.gene65949:CDS
TGCCGTGAAGACGGCGGATC+AGG 0.396893 1.1:+27044057 MS.gene65949:CDS
GGATATTTAGCAGGATCAAC+AGG 0.402646 1.1:-27044345 None:intergenic
AAACATGCTGGATTCGGTCT+TGG 0.405121 1.1:+27045479 MS.gene65949:CDS
TATGCAATTTATGGCTGATA+AGG 0.406440 1.1:+27045034 MS.gene65949:CDS
GCGGTCAGAGAATTCAGCTT+TGG 0.406711 1.1:-27044013 None:intergenic
AACTTGAAGGTTACCGGCAC+AGG 0.417890 1.1:-27044175 None:intergenic
CGGCAGATTATGGATTGAAT+CGG 0.418024 1.1:-27044039 None:intergenic
AGAGGGTTACCGTGGATAAC+TGG 0.418145 1.1:-27043975 None:intergenic
CCGTCCTTTCGAGGAATATC+GGG 0.423115 1.1:-27044738 None:intergenic
TCCGTCGTTCAATTCCAAAA+AGG 0.425770 1.1:-27044151 None:intergenic
TTAAGGAGAAGGGGATAAGA+AGG 0.425964 1.1:+27044604 MS.gene65949:CDS
CAATCAATAGTCGTGACAAT+TGG 0.426024 1.1:-27044525 None:intergenic
CCAATTAACTCTCCAACCTT+TGG 0.429345 1.1:-27045344 None:intergenic
AAAGGCGAAAGAACCCTTAT+CGG 0.430291 1.1:-27044133 None:intergenic
CATCATAACGCTCCTCCCTT+TGG 0.431653 1.1:-27045373 None:intergenic
CGGTCAGAGAATTCAGCTTT+GGG 0.433838 1.1:-27044012 None:intergenic
GGAACTTGTGTAGTTGTTGA+TGG 0.435212 1.1:+27044243 MS.gene65949:CDS
AACCGGAAGAAAAGCCGATA+AGG 0.438538 1.1:+27044119 MS.gene65949:CDS
TCTACCTTTCCGTCCTTTCG+AGG 0.444839 1.1:-27044747 None:intergenic
GATTCTCTTTGCTACCGGCC+TGG 0.445257 1.1:+27045514 MS.gene65949:CDS
CCAAAATTCAGCCAAATGCC+TGG 0.449352 1.1:-27044904 None:intergenic
GATTCGGTCTTGGCTTTGAA+AGG 0.456155 1.1:+27045489 MS.gene65949:CDS
TCACTGTTCCATACCGCCTC+AGG 0.466762 1.1:-27045457 None:intergenic
TCCATAATCTGCCGTGAAGA+CGG 0.470318 1.1:+27044048 MS.gene65949:CDS
ACAGTGAAACATGCTGGATT+CGG 0.470528 1.1:+27045473 MS.gene65949:CDS
AAGCTTCAACGATAACTTGA+AGG 0.473651 1.1:-27044188 None:intergenic
AACCTGATCCGCCGTCTTCA+CGG 0.474312 1.1:-27044059 None:intergenic
TTTCATAAATCTGCTCTGCC+AGG 0.478444 1.1:-27045572 None:intergenic
ATTCCCTTCCCACGATATCC+TGG 0.485303 1.1:+27045554 MS.gene65949:CDS
AGGTCAATGTACCATTCATA+TGG 0.485564 1.1:-27045437 None:intergenic
GATCGAGCTTCGCGCTGAAG+AGG 0.487208 1.1:+27044308 MS.gene65949:CDS
TTCTGTTTCGCTCCAGAATG+AGG 0.487604 1.1:-27044279 None:intergenic
TGTATATGTAAGTCGAATGA+AGG 0.488290 1.1:-27045102 None:intergenic
TTAGGCAAAGGATATTTAGC+AGG 0.488927 1.1:-27044354 None:intergenic
ATCGGAGGATGGGTGAAAAC+CGG 0.489249 1.1:+27044102 MS.gene65949:CDS
ATAGTCTATAATTATCCAAA+AGG 0.495051 1.1:+27045254 MS.gene65949:CDS
CCTAGAGACAGTGGCTGCTA+TGG 0.495878 1.1:+27045310 MS.gene65949:CDS
GCTGAAGAGGTTCTTCATGT+TGG 0.501465 1.1:+27044321 MS.gene65949:CDS
TACATTGACCTGAGGCGGTA+TGG 0.502024 1.1:+27045449 MS.gene65949:CDS
AATTCTCTGCACGAAATGTC+GGG 0.502631 1.1:-27044872 None:intergenic
GGATAATTATAGACTATGAC+AGG 0.507099 1.1:-27045248 None:intergenic
CCATAGCAGCCACTGTCTCT+AGG 0.509076 1.1:-27045310 None:intergenic
ATCTGCAAAAGCAGATAAGA+AGG 0.510339 1.1:+27044680 MS.gene65949:CDS
GAAGCTGTTGTTAAGGAGAA+GGG 0.510646 1.1:+27044594 MS.gene65949:CDS
TCATTGAGGCGCATGTAGAA+TGG 0.512501 1.1:-27045284 None:intergenic
GACGGCGGATCAGGTTTAGC+AGG 0.513325 1.1:+27044066 MS.gene65949:CDS
AGGATGATTCTCTTTGCTAC+CGG 0.513522 1.1:+27045509 MS.gene65949:CDS
CTGGAAGAATCTGCGAAGAA+TGG 0.519997 1.1:+27045140 MS.gene65949:CDS
GGGAACCTCGTCGGAGGAGA+GGG 0.522118 1.1:-27043992 None:intergenic
ACCGGAAGAAAAGCCGATAA+GGG 0.523007 1.1:+27044120 MS.gene65949:CDS
AAGCTGTTGTTAAGGAGAAG+GGG 0.524459 1.1:+27044595 MS.gene65949:CDS
CGATGTTGAAGCTGTTGTTA+AGG 0.528387 1.1:+27044587 MS.gene65949:CDS
GCAGGCAAGAAAGCTAGAAT+CGG 0.528940 1.1:+27044084 MS.gene65949:CDS
TGAAGCTGTTGTTAAGGAGA+AGG 0.529298 1.1:+27044593 MS.gene65949:CDS
TGTCATTCAACAAGGACTAA+AGG 0.529353 1.1:+27045394 MS.gene65949:CDS
AATCGCGTTTGCAGACTTGG+AGG 0.529982 1.1:+27044941 MS.gene65949:CDS
GGCAGATTATGGATTGAATC+GGG 0.531948 1.1:-27044038 None:intergenic
TGGGAACCTCGTCGGAGGAG+AGG 0.533868 1.1:-27043993 None:intergenic
AGAAAGCTAGAATCGGAGGA+TGG 0.535348 1.1:+27044091 MS.gene65949:CDS
TTGAACGACGGAACCTGTGC+CGG 0.538799 1.1:+27044162 MS.gene65949:CDS
GAATTCTCTGCACGAAATGT+CGG 0.541676 1.1:-27044873 None:intergenic
GATGTTCTTGTGCCAAAGGT+TGG 0.542344 1.1:+27045332 MS.gene65949:CDS
ACTATTGATTGCGAGGGTGC+TGG 0.546380 1.1:+27044537 MS.gene65949:CDS
AAGGTTGGAGAGTTAATTGG+TGG 0.550938 1.1:+27045347 MS.gene65949:CDS
AGATTCTTCCAGTATTTCCA+CGG 0.551909 1.1:-27045129 None:intergenic
TATGGATGTTCTTGTGCCAA+AGG 0.553956 1.1:+27045328 MS.gene65949:CDS
CCCGATATTCCTCGAAAGGA+CGG 0.554931 1.1:+27044738 MS.gene65949:CDS
AGTTAATTGGTGGAAGCCAA+AGG 0.559685 1.1:+27045357 MS.gene65949:CDS
TCTGCTCTGCCAGGATATCG+TGG 0.559764 1.1:-27045563 None:intergenic
ACACGATATCTGCTGTTGCT+CGG 0.565153 1.1:+27044433 MS.gene65949:CDS
TATGGAACAGTGAAACATGC+TGG 0.566811 1.1:+27045467 MS.gene65949:CDS
ATTGCGAGGGTGCTGGTGAA+AGG 0.569925 1.1:+27044544 MS.gene65949:CDS
TTCAGCTTTGGGAACCTCGT+CGG 0.571743 1.1:-27044001 None:intergenic
GAAAGCTAGAATCGGAGGAT+GGG 0.575623 1.1:+27044092 MS.gene65949:CDS
GTATATGTAAGTCGAATGAA+GGG 0.579832 1.1:-27045101 None:intergenic
GGCAAGAAAGCTAGAATCGG+AGG 0.582938 1.1:+27044087 MS.gene65949:CDS
TGCCTGTTAAGCCATATGAA+TGG 0.583784 1.1:+27045426 MS.gene65949:CDS
CTGCCAGGATATCGTGGGAA+GGG 0.584465 1.1:-27045557 None:intergenic
GAAGGAGATTAATGCTGCTG+AGG 0.586591 1.1:+27044698 MS.gene65949:CDS
AAATCACGCAAAACTTCAAG+AGG 0.593482 1.1:-27044387 None:intergenic
GTTACTTGACAACTGTCTTG+AGG 0.594479 1.1:+27045010 MS.gene65949:CDS
GTCACGACTATTGATTGCGA+GGG 0.597022 1.1:+27044531 MS.gene65949:CDS
GTTAATTGGTGGAAGCCAAA+GGG 0.599290 1.1:+27045358 MS.gene65949:CDS
GAATGGTCAGAAATTTGAGA+AGG 0.604552 1.1:+27045157 MS.gene65949:CDS
GGTCGGACGGTGGTGATGAG+AGG 0.605120 1.1:-27043954 None:intergenic
ACCGTGGATAACTGGTCGGA+CGG 0.609180 1.1:-27043967 None:intergenic
CTGTCTCTAGGTCGTCATTG+AGG 0.610010 1.1:-27045298 None:intergenic
GAAGGAGATTAGTGCTGCTG+TGG 0.610064 1.1:+27044622 MS.gene65949:CDS
CGTTATGATGTCATTCAACA+AGG 0.613502 1.1:+27045386 MS.gene65949:CDS
TCAGCCCGATATTCCTCGAA+AGG 0.613632 1.1:+27044734 MS.gene65949:CDS
TTATGGATTGAATCGGGACG+CGG 0.617154 1.1:-27044032 None:intergenic
AGCTTTGGGAACCTCGTCGG+AGG 0.618057 1.1:-27043998 None:intergenic
TGTCACGACTATTGATTGCG+AGG 0.620740 1.1:+27044530 MS.gene65949:CDS
CTGCTCTGCCAGGATATCGT+GGG 0.624533 1.1:-27045562 None:intergenic
TTTGAGAAGGAAGTAAAATG+GGG 0.626158 1.1:+27045170 MS.gene65949:CDS
TCTGCCAGGATATCGTGGGA+AGG 0.640927 1.1:-27045558 None:intergenic
AATGGTACATTGACCTGAGG+CGG 0.645218 1.1:+27045444 MS.gene65949:CDS
ACTTACATATACAGAAGCCG+TGG 0.654722 1.1:+27045112 MS.gene65949:CDS
ATAATCTGCCGTGAAGACGG+CGG 0.657362 1.1:+27044051 MS.gene65949:CDS
TATTCCTCGAAAGGACGGAA+AGG 0.658806 1.1:+27044743 MS.gene65949:CDS
GTGGATAACTGGTCGGACGG+TGG 0.662379 1.1:-27043964 None:intergenic
AGCAACAGCAGATATCGTGT+TGG 0.663258 1.1:-27044430 None:intergenic
ATATGTAAGTCGAATGAAGG+GGG 0.666943 1.1:-27045099 None:intergenic
ACCGTCCGACCAGTTATCCA+CGG 0.669853 1.1:+27043966 MS.gene65949:CDS
AATTGGTGGAAGCCAAAGGG+AGG 0.678168 1.1:+27045361 MS.gene65949:CDS
GTCGGAGGAGAGGGTTACCG+TGG 0.682551 1.1:-27043983 None:intergenic
ATGAATGGTACATTGACCTG+AGG 0.689555 1.1:+27045441 MS.gene65949:CDS
CAATGACGACCTAGAGACAG+TGG 0.692832 1.1:+27045301 MS.gene65949:CDS
TATATGTAAGTCGAATGAAG+GGG 0.702544 1.1:-27045100 None:intergenic
GGTTACCGTGGATAACTGGT+CGG 0.706814 1.1:-27043971 None:intergenic
GGTAACCCTCTCCTCCGACG+AGG 0.713572 1.1:+27043987 MS.gene65949:CDS
AATCACGCAAAACTTCAAGA+GGG 0.718553 1.1:-27044386 None:intergenic
GGATGATATGAAGTGCGCTG+AGG 0.752084 1.1:+27044962 MS.gene65949:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTCTATAATTATCCAAA+AGG + chr1.1:27045254-27045273 MS.gene65949:CDS 20.0%
! AATTTGAGAAGGAAGTAAAA+TGG + chr1.1:27045168-27045187 MS.gene65949:CDS 25.0%
! ATTTGAGAAGGAAGTAAAAT+GGG + chr1.1:27045169-27045188 MS.gene65949:CDS 25.0%
CTAAATATCCTTTGCCTAAA+AGG + chr1.1:27044358-27044377 MS.gene65949:CDS 30.0%
CTTAGTAGTGTGTATACATT+TGG + chr1.1:27044849-27044868 MS.gene65949:CDS 30.0%
GGATAATTATAGACTATGAC+AGG - chr1.1:27045251-27045270 None:intergenic 30.0%
GTATATGTAAGTCGAATGAA+GGG - chr1.1:27045104-27045123 None:intergenic 30.0%
TATATGTAAGTCGAATGAAG+GGG - chr1.1:27045103-27045122 None:intergenic 30.0%
TATGCAATTTATGGCTGATA+AGG + chr1.1:27045034-27045053 MS.gene65949:CDS 30.0%
TCTTGAGGATATGCAATTTA+TGG + chr1.1:27045025-27045044 MS.gene65949:CDS 30.0%
TGTATATGTAAGTCGAATGA+AGG - chr1.1:27045105-27045124 None:intergenic 30.0%
TTTGAGAAGGAAGTAAAATG+GGG + chr1.1:27045170-27045189 MS.gene65949:CDS 30.0%
! ATGTCAAATTTTTGTGTCAG+TGG + chr1.1:27044988-27045007 MS.gene65949:CDS 30.0%
! TAGAATGGTTTGATACCTTT+TGG - chr1.1:27045272-27045291 None:intergenic 30.0%
!! CAAGCATTTTTAACTGTTTC+TGG + chr1.1:27044795-27044814 MS.gene65949:CDS 30.0%
!! CATACATTTTTCAACAAGCA+TGG + chr1.1:27044483-27044502 MS.gene65949:CDS 30.0%
AAATCACGCAAAACTTCAAG+AGG - chr1.1:27044390-27044409 None:intergenic 35.0%
AACGATAACTTGAAGGTTAC+CGG - chr1.1:27044184-27044203 None:intergenic 35.0%
AAGCTTCAACGATAACTTGA+AGG - chr1.1:27044191-27044210 None:intergenic 35.0%
AATCACGCAAAACTTCAAGA+GGG - chr1.1:27044389-27044408 None:intergenic 35.0%
AGATTCTTCCAGTATTTCCA+CGG - chr1.1:27045132-27045151 None:intergenic 35.0%
AGGTCAATGTACCATTCATA+TGG - chr1.1:27045440-27045459 None:intergenic 35.0%
ATATGTAAGTCGAATGAAGG+GGG - chr1.1:27045102-27045121 None:intergenic 35.0%
ATCTGCAAAAGCAGATAAGA+AGG + chr1.1:27044680-27044699 MS.gene65949:CDS 35.0%
CAATCAATAGTCGTGACAAT+TGG - chr1.1:27044528-27044547 None:intergenic 35.0%
CGTTATGATGTCATTCAACA+AGG + chr1.1:27045386-27045405 MS.gene65949:CDS 35.0%
GAATGGTCAGAAATTTGAGA+AGG + chr1.1:27045157-27045176 MS.gene65949:CDS 35.0%
GTGGATGAACTTAAAAAAGC+TGG + chr1.1:27044641-27044660 MS.gene65949:CDS 35.0%
TACCATTCATATGGCTTAAC+AGG - chr1.1:27045431-27045450 None:intergenic 35.0%
TCTTATACTTCGTCTCTGTT+AGG - chr1.1:27045226-27045245 None:intergenic 35.0%
TGTCATTCAACAAGGACTAA+AGG + chr1.1:27045394-27045413 MS.gene65949:CDS 35.0%
TTAGGCAAAGGATATTTAGC+AGG - chr1.1:27044357-27044376 None:intergenic 35.0%
! CTTAGACAACTTGTTCTTAC+TGG + chr1.1:27044222-27044241 MS.gene65949:CDS 35.0%
! TCACATCTCGAATATTTTCC+AGG - chr1.1:27045535-27045554 None:intergenic 35.0%
! TTGCATCGATCGTTTGAAAA+TGG + chr1.1:27045067-27045086 MS.gene65949:CDS 35.0%
!! ATGGCTGATAAGGTTGATAA+AGG + chr1.1:27045044-27045063 MS.gene65949:CDS 35.0%
AAAGGCGAAAGAACCCTTAT+CGG - chr1.1:27044136-27044155 None:intergenic 40.0%
AAGCTGTTGTTAAGGAGAAG+GGG + chr1.1:27044595-27044614 MS.gene65949:CDS 40.0%
AAGGTTGGAGAGTTAATTGG+TGG + chr1.1:27045347-27045366 MS.gene65949:CDS 40.0%
AATTCTCTGCACGAAATGTC+GGG - chr1.1:27044875-27044894 None:intergenic 40.0%
ACAGTGAAACATGCTGGATT+CGG + chr1.1:27045473-27045492 MS.gene65949:CDS 40.0%
ACTTACATATACAGAAGCCG+TGG + chr1.1:27045112-27045131 MS.gene65949:CDS 40.0%
AGGATGATTCTCTTTGCTAC+CGG + chr1.1:27045509-27045528 MS.gene65949:CDS 40.0%
AGTTAATTGGTGGAAGCCAA+AGG + chr1.1:27045357-27045376 MS.gene65949:CDS 40.0%
ATGAATGGTACATTGACCTG+AGG + chr1.1:27045441-27045460 MS.gene65949:CDS 40.0%
CATCTCAAACTTCCTCATTC+TGG + chr1.1:27044267-27044286 MS.gene65949:CDS 40.0%
CCAAAGGTTGGAGAGTTAAT+TGG + chr1.1:27045344-27045363 MS.gene65949:CDS 40.0%
CCAATTAACTCTCCAACCTT+TGG - chr1.1:27045347-27045366 None:intergenic 40.0%
CGATGTTGAAGCTGTTGTTA+AGG + chr1.1:27044587-27044606 MS.gene65949:CDS 40.0%
GAAGCTGTTGTTAAGGAGAA+GGG + chr1.1:27044594-27044613 MS.gene65949:CDS 40.0%
GAATTCTCTGCACGAAATGT+CGG - chr1.1:27044876-27044895 None:intergenic 40.0%
GGAACTTGTGTAGTTGTTGA+TGG + chr1.1:27044243-27044262 MS.gene65949:CDS 40.0%
GGATATTTAGCAGGATCAAC+AGG - chr1.1:27044348-27044367 None:intergenic 40.0%
GTTAATTGGTGGAAGCCAAA+GGG + chr1.1:27045358-27045377 MS.gene65949:CDS 40.0%
GTTACTTGACAACTGTCTTG+AGG + chr1.1:27045010-27045029 MS.gene65949:CDS 40.0%
TATGGAACAGTGAAACATGC+TGG + chr1.1:27045467-27045486 MS.gene65949:CDS 40.0%
TATGGATGTTCTTGTGCCAA+AGG + chr1.1:27045328-27045347 MS.gene65949:CDS 40.0%
TCCGTCGTTCAATTCCAAAA+AGG - chr1.1:27044154-27044173 None:intergenic 40.0%
TGAAGCTGTTGTTAAGGAGA+AGG + chr1.1:27044593-27044612 MS.gene65949:CDS 40.0%
TGCCTGTTAAGCCATATGAA+TGG + chr1.1:27045426-27045445 MS.gene65949:CDS 40.0%
TTAAGGAGAAGGGGATAAGA+AGG + chr1.1:27044604-27044623 MS.gene65949:CDS 40.0%
! ATCTCGAATATTTTCCAGGC+CGG - chr1.1:27045531-27045550 None:intergenic 40.0%
! CGGCAGATTATGGATTGAAT+CGG - chr1.1:27044042-27044061 None:intergenic 40.0%
! GCATTTGGCTGAATTTTGGA+TGG + chr1.1:27044908-27044927 MS.gene65949:CDS 40.0%
! GGCAGATTATGGATTGAATC+GGG - chr1.1:27044041-27044060 None:intergenic 40.0%
! TAAGGGTTCTTTCGCCTTTT+TGG + chr1.1:27044137-27044156 MS.gene65949:CDS 40.0%
!!! GCCTTTTTGGAATTGAACGA+CGG + chr1.1:27044150-27044169 MS.gene65949:CDS 40.0%
AACCGGAAGAAAAGCCGATA+AGG + chr1.1:27044119-27044138 MS.gene65949:CDS 45.0%
AAGTCTGCAAACGCGATTTC+AGG - chr1.1:27044939-27044958 None:intergenic 45.0%
AATGGTACATTGACCTGAGG+CGG + chr1.1:27045444-27045463 MS.gene65949:CDS 45.0%
ACACGATATCTGCTGTTGCT+CGG + chr1.1:27044433-27044452 MS.gene65949:CDS 45.0%
ACCGGAAGAAAAGCCGATAA+GGG + chr1.1:27044120-27044139 MS.gene65949:CDS 45.0%
AGAAAGCTAGAATCGGAGGA+TGG + chr1.1:27044091-27044110 MS.gene65949:CDS 45.0%
AGCAACAGCAGATATCGTGT+TGG - chr1.1:27044433-27044452 None:intergenic 45.0%
AGGAATATCGGGCTGAAGTT+TGG - chr1.1:27044730-27044749 None:intergenic 45.0%
CAGAGAATTCTCACACTTCC+AGG + chr1.1:27044886-27044905 MS.gene65949:CDS 45.0%
CCAAAATTCAGCCAAATGCC+TGG - chr1.1:27044907-27044926 None:intergenic 45.0%
CGGTCAGAGAATTCAGCTTT+GGG - chr1.1:27044015-27044034 None:intergenic 45.0%
CTGGAAGAATCTGCGAAGAA+TGG + chr1.1:27045140-27045159 MS.gene65949:CDS 45.0%
GAAAGCTAGAATCGGAGGAT+GGG + chr1.1:27044092-27044111 MS.gene65949:CDS 45.0%
GAAGGAGATTAATGCTGCTG+AGG + chr1.1:27044698-27044717 MS.gene65949:CDS 45.0%
GATGTTCTTGTGCCAAAGGT+TGG + chr1.1:27045332-27045351 MS.gene65949:CDS 45.0%
GCAGGCAAGAAAGCTAGAAT+CGG + chr1.1:27044084-27044103 MS.gene65949:CDS 45.0%
GCTGAAGAGGTTCTTCATGT+TGG + chr1.1:27044321-27044340 MS.gene65949:CDS 45.0%
TACAGAAGCCGTGGAAATAC+TGG + chr1.1:27045121-27045140 MS.gene65949:CDS 45.0%
TATTCCTCGAAAGGACGGAA+AGG + chr1.1:27044743-27044762 MS.gene65949:CDS 45.0%
TCATTGAGGCGCATGTAGAA+TGG - chr1.1:27045287-27045306 None:intergenic 45.0%
TCCATAATCTGCCGTGAAGA+CGG + chr1.1:27044048-27044067 MS.gene65949:CDS 45.0%
TCCGTCCTTTCGAGGAATAT+CGG - chr1.1:27044742-27044761 None:intergenic 45.0%
TGAAATCGCGTTTGCAGACT+TGG + chr1.1:27044938-27044957 MS.gene65949:CDS 45.0%
TTCTCACACTTCCAGGCATT+TGG + chr1.1:27044893-27044912 MS.gene65949:CDS 45.0%
TTCTGTTTCGCTCCAGAATG+AGG - chr1.1:27044282-27044301 None:intergenic 45.0%
! AAACATGCTGGATTCGGTCT+TGG + chr1.1:27045479-27045498 MS.gene65949:CDS 45.0%
! CCAGGCATTTGGCTGAATTT+TGG + chr1.1:27044904-27044923 MS.gene65949:CDS 45.0%
! GTCACGACTATTGATTGCGA+GGG + chr1.1:27044531-27044550 MS.gene65949:CDS 45.0%
! TCAAGAGGGAGTCTCCTTTT+AGG - chr1.1:27044375-27044394 None:intergenic 45.0%
! TGTCACGACTATTGATTGCG+AGG + chr1.1:27044530-27044549 MS.gene65949:CDS 45.0%
! TTATGGATTGAATCGGGACG+CGG - chr1.1:27044035-27044054 None:intergenic 45.0%
!! AGCAGATATCGTGTTGGTTC+TGG - chr1.1:27044427-27044446 None:intergenic 45.0%
!! GATTCGGTCTTGGCTTTGAA+AGG + chr1.1:27045489-27045508 MS.gene65949:CDS 45.0%
!!! ACCCTTATCGGCTTTTCTTC+CGG - chr1.1:27044124-27044143 None:intergenic 45.0%
AACTTGAAGGTTACCGGCAC+AGG - chr1.1:27044178-27044197 None:intergenic 50.0%
AATCGCGTTTGCAGACTTGG+AGG + chr1.1:27044941-27044960 MS.gene65949:CDS 50.0%
AATTGGTGGAAGCCAAAGGG+AGG + chr1.1:27045361-27045380 MS.gene65949:CDS 50.0%
AGAGGGTTACCGTGGATAAC+TGG - chr1.1:27043978-27043997 None:intergenic 50.0%
ATAATCTGCCGTGAAGACGG+CGG + chr1.1:27044051-27044070 MS.gene65949:CDS 50.0%
ATCGGAGGATGGGTGAAAAC+CGG + chr1.1:27044102-27044121 MS.gene65949:CDS 50.0%
ATTCCCTTCCCACGATATCC+TGG + chr1.1:27045554-27045573 MS.gene65949:CDS 50.0%
CAATGACGACCTAGAGACAG+TGG + chr1.1:27045301-27045320 MS.gene65949:CDS 50.0%
CATCATAACGCTCCTCCCTT+TGG - chr1.1:27045376-27045395 None:intergenic 50.0%
CCCGATATTCCTCGAAAGGA+CGG + chr1.1:27044738-27044757 MS.gene65949:CDS 50.0%
CCGTCCTTTCGAGGAATATC+GGG - chr1.1:27044741-27044760 None:intergenic 50.0%
CTGTCTCTAGGTCGTCATTG+AGG - chr1.1:27045301-27045320 None:intergenic 50.0%
GCGGTCAGAGAATTCAGCTT+TGG - chr1.1:27044016-27044035 None:intergenic 50.0%
GGATGATATGAAGTGCGCTG+AGG + chr1.1:27044962-27044981 MS.gene65949:CDS 50.0%
GGCAAGAAAGCTAGAATCGG+AGG + chr1.1:27044087-27044106 MS.gene65949:CDS 50.0%
GGTTACCGTGGATAACTGGT+CGG - chr1.1:27043974-27043993 None:intergenic 50.0%
TACATTGACCTGAGGCGGTA+TGG + chr1.1:27045449-27045468 MS.gene65949:CDS 50.0%
TCAGCCCGATATTCCTCGAA+AGG + chr1.1:27044734-27044753 MS.gene65949:CDS 50.0%
TCTACCTTTCCGTCCTTTCG+AGG - chr1.1:27044750-27044769 None:intergenic 50.0%
TGTAAGTCGAATGAAGGGGG+TGG - chr1.1:27045099-27045118 None:intergenic 50.0%
! ACTATTGATTGCGAGGGTGC+TGG + chr1.1:27044537-27044556 MS.gene65949:CDS 50.0%
! GAAGGAGATTAGTGCTGCTG+TGG + chr1.1:27044622-27044641 MS.gene65949:CDS 50.0%
! GGGAGTCTCCTTTTAGGCAA+AGG - chr1.1:27044369-27044388 None:intergenic 50.0%
!! TTCAGCTTTGGGAACCTCGT+CGG - chr1.1:27044004-27044023 None:intergenic 50.0%
AACCTGATCCGCCGTCTTCA+CGG - chr1.1:27044062-27044081 None:intergenic 55.0%
AAGTCGAATGAAGGGGGTGG+AGG - chr1.1:27045096-27045115 None:intergenic 55.0%
ACCGTCCGACCAGTTATCCA+CGG + chr1.1:27043966-27043985 MS.gene65949:CDS 55.0%
ACCGTGGATAACTGGTCGGA+CGG - chr1.1:27043970-27043989 None:intergenic 55.0%
CCATAGCAGCCACTGTCTCT+AGG - chr1.1:27045313-27045332 None:intergenic 55.0%
CCTAGAGACAGTGGCTGCTA+TGG + chr1.1:27045310-27045329 MS.gene65949:CDS 55.0%
CTGCCAGGATATCGTGGGAA+GGG - chr1.1:27045560-27045579 None:intergenic 55.0%
CTGCTCTGCCAGGATATCGT+GGG - chr1.1:27045565-27045584 None:intergenic 55.0%
GATTCTCTTTGCTACCGGCC+TGG + chr1.1:27045514-27045533 MS.gene65949:CDS 55.0%
GCCGTCTTCACGGCAGATTA+TGG - chr1.1:27044052-27044071 None:intergenic 55.0%
TCACTGTTCCATACCGCCTC+AGG - chr1.1:27045460-27045479 None:intergenic 55.0%
TCTGCCAGGATATCGTGGGA+AGG - chr1.1:27045561-27045580 None:intergenic 55.0%
TCTGCTCTGCCAGGATATCG+TGG - chr1.1:27045566-27045585 None:intergenic 55.0%
TTGAACGACGGAACCTGTGC+CGG + chr1.1:27044162-27044181 MS.gene65949:CDS 55.0%
!! ATTGCGAGGGTGCTGGTGAA+AGG + chr1.1:27044544-27044563 MS.gene65949:CDS 55.0%
GACGGCGGATCAGGTTTAGC+AGG + chr1.1:27044066-27044085 MS.gene65949:CDS 60.0%
GATCGAGCTTCGCGCTGAAG+AGG + chr1.1:27044308-27044327 MS.gene65949:CDS 60.0%
GTGGATAACTGGTCGGACGG+TGG - chr1.1:27043967-27043986 None:intergenic 60.0%
!! AGCTTTGGGAACCTCGTCGG+AGG - chr1.1:27044001-27044020 None:intergenic 60.0%
GGGAACCTCGTCGGAGGAGA+GGG - chr1.1:27043995-27044014 None:intergenic 65.0%
GGTAACCCTCTCCTCCGACG+AGG + chr1.1:27043987-27044006 MS.gene65949:CDS 65.0%
GTCGGAGGAGAGGGTTACCG+TGG - chr1.1:27043986-27044005 None:intergenic 65.0%
TGCCGTGAAGACGGCGGATC+AGG + chr1.1:27044057-27044076 MS.gene65949:CDS 65.0%
TGGGAACCTCGTCGGAGGAG+AGG - chr1.1:27043996-27044015 None:intergenic 65.0%
! GGTCGGACGGTGGTGATGAG+AGG - chr1.1:27043957-27043976 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 27043946 27045592 27043946 ID=MS.gene65949
chr1.1 mRNA 27043946 27045592 27043946 ID=MS.gene65949.t1;Parent=MS.gene65949
chr1.1 exon 27043946 27045592 27043946 ID=MS.gene65949.t1.exon1;Parent=MS.gene65949.t1
chr1.1 CDS 27043946 27045592 27043946 ID=cds.MS.gene65949.t1;Parent=MS.gene65949.t1
Gene Sequence

>MS.gene65949

ATGTCAGACCTCTCATCACCACCGTCCGACCAGTTATCCACGGTAACCCTCTCCTCCGACGAGGTTCCCAAAGCTGAATTCTCTGACCGCGTCCCGATTCAATCCATAATCTGCCGTGAAGACGGCGGATCAGGTTTAGCAGGCAAGAAAGCTAGAATCGGAGGATGGGTGAAAACCGGAAGAAAAGCCGATAAGGGTTCTTTCGCCTTTTTGGAATTGAACGACGGAACCTGTGCCGGTAACCTTCAAGTTATCGTTGAAGCTTCGCTTGCTGATCTTAGACAACTTGTTCTTACTGGAACTTGTGTAGTTGTTGATGGACATCTCAAACTTCCTCATTCTGGAGCGAAACAGAAAAAGAAGATCGAGCTTCGCGCTGAAGAGGTTCTTCATGTTGGTCCTGTTGATCCTGCTAAATATCCTTTGCCTAAAAGGAGACTCCCTCTTGAAGTTTTGCGTGATTTCGTTCATCTTCGTTCCAGAACCAACACGATATCTGCTGTTGCTCGGATCAGTAATGCTCTTGCTTATGCTACACATACATTTTTCAACAAGCATGGTTTTCTTCATGTGCGCACGCCAATTGTCACGACTATTGATTGCGAGGGTGCTGGTGAAAGGTTTCAAGTGACAACTTTGTTCGATGTTGAAGCTGTTGTTAAGGAGAAGGGGATAAGAAGGAGATTAGTGCTGCTGTGGATGAACTTAAAAAAGCTGGTTGTTTCAAAATTGAAATCTGCAAAAGCAGATAAGAAGGAGATTAATGCTGCTGAGGAAAGATCCAAACTTCAGCCCGATATTCCTCGAAAGGACGGAAAGGTAGATTATACTCAAGATTTCTTTGCGCGTCAAGCATTTTTAACTGTTTCTGGTCAGCTTCAAGCTGAGTCTTATGCGTGTGCTCTTAGTAGTGTGTATACATTTGGCCCGACATTTCGTGCAGAGAATTCTCACACTTCCAGGCATTTGGCTGAATTTTGGATGGTAGAACCTGAAATCGCGTTTGCAGACTTGGAGGATGATATGAAGTGCGCTGAGGCATATGTCAAATTTTTGTGTCAGTGGTTACTTGACAACTGTCTTGAGGATATGCAATTTATGGCTGATAAGGTTGATAAAGGTTGCATCGATCGTTTGAAAATGGTTGCCTCCACCCCCTTCATTCGACTTACATATACAGAAGCCGTGGAAATACTGGAAGAATCTGCGAAGAATGGTCAGAAATTTGAGAAGGAAGTAAAATGGGGAATCGATTTAGCTTCTGAGCATGAAAGATACCTAACAGAGACGAAGTATAAGAAGCCTGTCATAGTCTATAATTATCCAAAAGGTATCAAACCATTCTACATGCGCCTCAATGACGACCTAGAGACAGTGGCTGCTATGGATGTTCTTGTGCCAAAGGTTGGAGAGTTAATTGGTGGAAGCCAAAGGGAGGAGCGTTATGATGTCATTCAACAAGGACTAAAGGAGAAGAATCTGCCTGTTAAGCCATATGAATGGTACATTGACCTGAGGCGGTATGGAACAGTGAAACATGCTGGATTCGGTCTTGGCTTTGAAAGGATGATTCTCTTTGCTACCGGCCTGGAAAATATTCGAGATGTGATTCCCTTCCCACGATATCCTGGCAGAGCAGATTTATGA
Protein sequence

>MS.gene65949.t1

MSDLSSPPSDQLSTVTLSSDEVPKAEFSDRVPIQSIICREDGGSGLAGKKARIGGWVKTGRKADKGSFAFLELNDGTCAGNLQVIVEASLADLRQLVLTGTCVVVDGHLKLPHSGAKQKKKIELRAEEVLHVGPVDPAKYPLPKRRLPLEVLRDFVHLRSRTNTISAVARISNALAYATHTFFNKHGFLHVRTPIVTTIDCEGAGERFQVTTLFDVEAVVKEKGIRRRLVLLWMNLKKLVVSKLKSAKADKKEINAAEERSKLQPDIPRKDGKVDYTQDFFARQAFLTVSGQLQAESYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMKCAEAYVKFLCQWLLDNCLEDMQFMADKVDKGCIDRLKMVASTPFIRLTYTEAVEILEESAKNGQKFEKEVKWGIDLASEHERYLTETKYKKPVIVYNYPKGIKPFYMRLNDDLETVAAMDVLVPKVGELIGGSQREERYDVIQQGLKEKNLPVKPYEWYIDLRRYGTVKHAGFGLGFERMILFATGLENIRDVIPFPRYPGRADL