AlfalfaGEDB | MS.gene67117

M. sativa cultivar XinJiangDaYe / MS.gene67117

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13202.9 PF13405.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67117.t1	XP_003589208.1	94.7	95	1	1	1	91	1	95	1.10E-42	182.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67117.t1	G7I3M4	94.7	95	1	1	1	91	1	95	7.9e-43	182.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050680	MS.gene67117	0.834389	3.08E-56	-1.69E-46
MS.gene050750	MS.gene67117	0.815909	7.26E-52	-1.69E-46
MS.gene050807	MS.gene67117	0.804661	1.94E-49	-1.69E-46
MS.gene050953	MS.gene67117	0.808726	2.69E-50	-1.69E-46
MS.gene051368	MS.gene67117	0.80132	9.52E-49	-1.69E-46
MS.gene052056	MS.gene67117	-0.809175	2.15E-50	-1.69E-46
MS.gene053166	MS.gene67117	0.827954	1.18E-54	-1.69E-46
MS.gene053226	MS.gene67117	0.801259	9.80E-49	-1.69E-46
MS.gene05415	MS.gene67117	0.807484	4.94E-50	-1.69E-46
MS.gene054291	MS.gene67117	-0.80057	1.35E-48	-1.69E-46
MS.gene054405	MS.gene67117	0.804352	2.25E-49	-1.69E-46
MS.gene054559	MS.gene67117	0.805728	1.16E-49	-1.69E-46
MS.gene054657	MS.gene67117	0.821818	3.30E-53	-1.69E-46
MS.gene054775	MS.gene67117	0.803407	3.54E-49	-1.69E-46
MS.gene05510	MS.gene67117	0.814984	1.17E-51	-1.69E-46
MS.gene055227	MS.gene67117	0.809298	2.03E-50	-1.69E-46
MS.gene055623	MS.gene67117	0.804843	1.78E-49	-1.69E-46
MS.gene055701	MS.gene67117	0.857167	1.96E-62	-1.69E-46
MS.gene055818	MS.gene67117	0.812042	5.17E-51	-1.69E-46
MS.gene055926	MS.gene67117	0.814859	1.24E-51	-1.69E-46
MS.gene05604	MS.gene67117	0.807913	4.01E-50	-1.69E-46
MS.gene056366	MS.gene67117	0.803026	4.24E-49	-1.69E-46
MS.gene057060	MS.gene67117	0.8008	1.22E-48	-1.69E-46
MS.gene057208	MS.gene67117	0.810834	9.46E-51	-1.69E-46
MS.gene057693	MS.gene67117	0.806025	1.01E-49	-1.69E-46
MS.gene05847	MS.gene67117	0.805235	1.47E-49	-1.69E-46
MS.gene059161	MS.gene67117	0.816519	5.30E-52	-1.69E-46
MS.gene059382	MS.gene67117	0.805931	1.05E-49	-1.69E-46
MS.gene059609	MS.gene67117	0.828624	8.10E-55	-1.69E-46
MS.gene060137	MS.gene67117	-0.802285	6.03E-49	-1.69E-46
MS.gene060322	MS.gene67117	0.800242	1.58E-48	-1.69E-46
MS.gene060802	MS.gene67117	0.821756	3.41E-53	-1.69E-46
MS.gene060803	MS.gene67117	0.826537	2.57E-54	-1.69E-46
MS.gene060998	MS.gene67117	0.80186	7.38E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67117.t1	MTR_1g019640	94.737	95	1	1	1	91	1	95	4.40e-61	181
MS.gene67117.t1	MTR_1g019660	89.474	95	6	1	1	91	1	95	3.89e-55	166
MS.gene67117.t1	MTR_1g019610	86.316	95	9	1	1	91	1	95	1.94e-54	164
MS.gene67117.t1	MTR_1g019670	65.591	93	29	1	1	90	1	93	1.47e-38	124
MS.gene67117.t1	MTR_1g019600	45.000	100	46	1	1	91	1	100	3.18e-19	75.5
MS.gene67117.t1	MTR_1g019590	43.678	87	45	1	5	91	21	103	2.55e-16	68.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTGATTAGTCCGTCTTTAT+TGG	0.241517	1.3:+3957550	None:intergenic
TATCTCCTTACCCGTGGTTT+TGG	0.273881	1.3:-3957500	MS.gene67117:CDS
TGATCCATAGACACCCTCTT+TGG	0.332546	1.3:+3957728	None:intergenic
TATCCCAAAGAGGGTGTCTA+TGG	0.383107	1.3:-3957732	MS.gene67117:CDS
GATCCATAGACACCCTCTTT+GGG	0.423736	1.3:+3957729	None:intergenic
CAACGATTGGCTCTCCATCC+AGG	0.437823	1.3:+3957590	None:intergenic
AGCTTTCATCAAACAACGAT+TGG	0.498009	1.3:+3957577	None:intergenic
TCTTGCCAAAACCACGGGTA+AGG	0.519368	1.3:+3957495	None:intergenic
CGATGGTCGTTACACAAAGA+AGG	0.521782	1.3:-3957651	MS.gene67117:CDS
TAGGTTCTTACTACCCTGGA+TGG	0.528156	1.3:-3957604	MS.gene67117:CDS
TTAGTTCTTGCCAAAACCAC+GGG	0.528157	1.3:+3957490	None:intergenic
TGGATCAAATCAACTTTGAA+AGG	0.536831	1.3:-3957712	MS.gene67117:CDS
GATTTAGGTTCTTACTACCC+TGG	0.543944	1.3:-3957608	MS.gene67117:CDS
AACGATTGGCTCTCCATCCA+GGG	0.580860	1.3:+3957591	None:intergenic
ATTAGTTCTTGCCAAAACCA+CGG	0.592102	1.3:+3957489	None:intergenic
GCCTGTGTTTATCCCAAAGA+GGG	0.602626	1.3:-3957741	MS.gene67117:CDS
ATTGATTATCTCCTTACCCG+TGG	0.619921	1.3:-3957506	MS.gene67117:CDS
AAAGCTGATGCCAATAAAGA+CGG	0.628000	1.3:-3957560	MS.gene67117:CDS
TGCCTGTGTTTATCCCAAAG+AGG	0.630941	1.3:-3957742	MS.gene67117:CDS
ACCCTCTTTGGGATAAACAC+AGG	0.645492	1.3:+3957740	None:intergenic
AGACGGACTAATCAGTGGCG+AGG	0.659853	1.3:-3957543	MS.gene67117:CDS
AATAAAGACGGACTAATCAG+TGG	0.668016	1.3:-3957548	MS.gene67117:CDS
AAGGCGGATATCAATAACGA+TGG	0.697636	1.3:-3957668	MS.gene67117:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGGAACATTATAGAAAAAT+TGG	-	chr1.3:3957540-3957559	MS.gene67117:CDS	20.0%
!	CATTATAGAAAAATTGGAGA+AGG	-	chr1.3:3957546-3957565	MS.gene67117:CDS	25.0%
!!!	AAAAAAGCTCTAAAGGATTT+AGG	-	chr1.3:3957610-3957629	MS.gene67117:CDS	25.0%
	TATAGAAAAATTGGAGAAGG+CGG	-	chr1.3:3957549-3957568	MS.gene67117:CDS	30.0%
	TGGATCAAATCAACTTTGAA+AGG	-	chr1.3:3957521-3957540	MS.gene67117:CDS	30.0%
	AAAGCTGATGCCAATAAAGA+CGG	-	chr1.3:3957673-3957692	MS.gene67117:CDS	35.0%
	AGCTTTCATCAAACAACGAT+TGG	+	chr1.3:3957659-3957678	None:intergenic	35.0%
!	ACTGATTAGTCCGTCTTTAT+TGG	+	chr1.3:3957686-3957705	None:intergenic	35.0%
!	GGAACTCAAAAAAGCTCTAA+AGG	-	chr1.3:3957603-3957622	MS.gene67117:CDS	35.0%
!!	AATAAAGACGGACTAATCAG+TGG	-	chr1.3:3957685-3957704	MS.gene67117:CDS	35.0%
	AAGGCGGATATCAATAACGA+TGG	-	chr1.3:3957565-3957584	MS.gene67117:CDS	40.0%
	ATTGATTATCTCCTTACCCG+TGG	-	chr1.3:3957727-3957746	MS.gene67117:CDS	40.0%
!	GATTTAGGTTCTTACTACCC+TGG	-	chr1.3:3957625-3957644	MS.gene67117:CDS	40.0%
!	TTAGTTCTTGCCAAAACCAC+GGG	+	chr1.3:3957746-3957765	None:intergenic	40.0%
	CGATGGTCGTTACACAAAGA+AGG	-	chr1.3:3957582-3957601	MS.gene67117:CDS	45.0%
	GATCCATAGACACCCTCTTT+GGG	+	chr1.3:3957507-3957526	None:intergenic	45.0%
	GCCTGTGTTTATCCCAAAGA+GGG	-	chr1.3:3957492-3957511	MS.gene67117:CDS	45.0%
	TAGGTTCTTACTACCCTGGA+TGG	-	chr1.3:3957629-3957648	MS.gene67117:CDS	45.0%
	TGATCCATAGACACCCTCTT+TGG	+	chr1.3:3957508-3957527	None:intergenic	45.0%
	TGCCTGTGTTTATCCCAAAG+AGG	-	chr1.3:3957491-3957510	MS.gene67117:CDS	45.0%
!	TATCTCCTTACCCGTGGTTT+TGG	-	chr1.3:3957733-3957752	MS.gene67117:CDS	45.0%
!!	ACCCTCTTTGGGATAAACAC+AGG	+	chr1.3:3957496-3957515	None:intergenic	45.0%
!!	TATCCCAAAGAGGGTGTCTA+TGG	-	chr1.3:3957501-3957520	MS.gene67117:CDS	45.0%
	TCTTGCCAAAACCACGGGTA+AGG	+	chr1.3:3957741-3957760	None:intergenic	50.0%
!	AACGATTGGCTCTCCATCCA+GGG	+	chr1.3:3957645-3957664	None:intergenic	50.0%
!	CAACGATTGGCTCTCCATCC+AGG	+	chr1.3:3957646-3957665	None:intergenic	55.0%
!!	AGACGGACTAATCAGTGGCG+AGG	-	chr1.3:3957690-3957709	MS.gene67117:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	3957490	3957765	3957490	ID=MS.gene67117
chr1.3	mRNA	3957490	3957765	3957490	ID=MS.gene67117.t1;Parent=MS.gene67117
chr1.3	exon	3957490	3957765	3957490	ID=MS.gene67117.t1.exon1;Parent=MS.gene67117.t1
chr1.3	CDS	3957490	3957765	3957490	ID=cds.MS.gene67117.t1;Parent=MS.gene67117.t1

Gene Sequence	>MS.gene67117 ATGCCTGTGTTTATCCCAAAGAGGGTGTCTATGGATCAAATCAACTTTGAAAGGAACATTATAGAAAAATTGGAGAAGGCGGATATCAATAACGATGGTCGTTACACAAAGAAGGAACTCAAAAAAGCTCTAAAGGATTTAGGTTCTTACTACCCTGGATGGAGAGCCAATCGTTGTTTGATGAAAGCTGATGCCAATAAAGACGGACTAATCAGTGGCGAGGAAATCGACACTTTAATTGATTATCTCCTTACCCGTGGTTTTGGCAAGAACTAA
Protein sequence	>MS.gene67117.t1 MPVFIPKRVSMDQINFERNIIEKLEKADINNDGRYTKKELKKALKDLGSYYPGWRANRCLMKADANKDGLISGEEIDTLIDYLLTRGFGKN