Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67136.t1 | XP_003589185.1 | 99.2 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 5.00E-62 | 246.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67136.t1 | P97852 | 39.1 | 92 | 51 | 3 | 24 | 113 | 636 | 724 | 9.9e-11 | 67.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67136.t1 | G7I3K1 | 99.2 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 3.6e-62 | 246.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051452 | MS.gene67136 | 0.802167 | 6.38E-49 | -1.69E-46 |
| MS.gene051655 | MS.gene67136 | 0.803218 | 3.87E-49 | -1.69E-46 |
| MS.gene051975 | MS.gene67136 | 0.813579 | 2.38E-51 | -1.69E-46 |
| MS.gene052824 | MS.gene67136 | 0.802601 | 5.19E-49 | -1.69E-46 |
| MS.gene052954 | MS.gene67136 | 0.845194 | 4.73E-59 | -1.69E-46 |
| MS.gene053045 | MS.gene67136 | 0.807303 | 5.40E-50 | -1.69E-46 |
| MS.gene053189 | MS.gene67136 | 0.831793 | 1.36E-55 | -1.69E-46 |
| MS.gene053216 | MS.gene67136 | 0.807438 | 5.05E-50 | -1.69E-46 |
| MS.gene053298 | MS.gene67136 | 0.814876 | 1.23E-51 | -1.69E-46 |
| MS.gene053963 | MS.gene67136 | 0.820027 | 8.52E-53 | -1.69E-46 |
| MS.gene054536 | MS.gene67136 | 0.807733 | 4.38E-50 | -1.69E-46 |
| MS.gene05469 | MS.gene67136 | 0.809073 | 2.27E-50 | -1.69E-46 |
| MS.gene055841 | MS.gene67136 | 0.812585 | 3.94E-51 | -1.69E-46 |
| MS.gene056787 | MS.gene67136 | 0.809568 | 1.77E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene67136 | 0.830346 | 3.09E-55 | -1.69E-46 |
| MS.gene05795 | MS.gene67136 | 0.827021 | 1.97E-54 | -1.69E-46 |
| MS.gene057999 | MS.gene67136 | 0.835908 | 1.28E-56 | -1.69E-46 |
| MS.gene058238 | MS.gene67136 | 0.812005 | 5.27E-51 | -1.69E-46 |
| MS.gene059830 | MS.gene67136 | 0.825686 | 4.09E-54 | -1.69E-46 |
| MS.gene060297 | MS.gene67136 | 0.828321 | 9.59E-55 | -1.69E-46 |
| MS.gene060365 | MS.gene67136 | 0.806112 | 9.64E-50 | -1.69E-46 |
| MS.gene060586 | MS.gene67136 | 0.80746 | 5.00E-50 | -1.69E-46 |
| MS.gene060879 | MS.gene67136 | 0.805833 | 1.10E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67136.t1 | MTR_1g019430 | 99.200 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 8.01e-88 | 251 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67136.t1 | AT5G42890 | 79.508 | 122 | 25 | 0 | 4 | 125 | 2 | 123 | 1.17e-68 | 202 |
Find 21 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGGGTGCAATGAAGATTA+AGG | 0.247988 | 1.3:-4060571 | MS.gene67136:CDS |
| GCTCACATCTTGGAAGGCTT+TGG | 0.309953 | 1.3:+4060507 | None:intergenic |
| GACTTTGTTAAGGTTGCATT+AGG | 0.316740 | 1.3:-4060727 | MS.gene67136:CDS |
| CTCACATCTTGGAAGGCTTT+GGG | 0.353213 | 1.3:+4060508 | None:intergenic |
| TCAGTTTCAAATTGCTCCTA+AGG | 0.429160 | 1.3:-4061731 | MS.gene67136:intron |
| TACAGATGAAGACTTTGTTA+AGG | 0.431661 | 1.3:-4060737 | MS.gene67136:CDS |
| GAAGGCTTTGGGAAGATATC+CGG | 0.454252 | 1.3:+4060519 | None:intergenic |
| AGACATCTCTGCTCACATCT+TGG | 0.471053 | 1.3:+4060497 | None:intergenic |
| AGGGGTGCAATGAAGATTAA+GGG | 0.479063 | 1.3:-4060570 | MS.gene67136:CDS |
| ACTTTGTTAAGGTTGCATTA+GGG | 0.521418 | 1.3:-4060726 | MS.gene67136:CDS |
| TTCTCCTTTCTTGAGATCAA+CGG | 0.549718 | 1.3:+4061590 | None:intergenic |
| TATATAGGGGCATATGAAGG+TGG | 0.550169 | 1.3:-4060784 | MS.gene67136:intron |
| GTGTATATAGGGGCATATGA+AGG | 0.563217 | 1.3:-4060787 | MS.gene67136:intron |
| TATACCGTTGATCTCAAGAA+AGG | 0.565878 | 1.3:-4061594 | MS.gene67136:CDS |
| ATATAGGGGCATATGAAGGT+GGG | 0.567168 | 1.3:-4060783 | MS.gene67136:intron |
| ATCCACAGATTGCTTTCATG+AGG | 0.575757 | 1.3:-4060696 | MS.gene67136:intron |
| ATCTCTGCTCACATCTTGGA+AGG | 0.575766 | 1.3:+4060501 | None:intergenic |
| TGCTGCTCAGAAATTTACTC+CGG | 0.597894 | 1.3:-4060538 | MS.gene67136:CDS |
| AACCTCATGAAAGCAATCTG+TGG | 0.609703 | 1.3:+4060694 | None:intergenic |
| ATTCGATGATGAATCAGCCA+TGG | 0.650427 | 1.3:+4061843 | None:intergenic |
| AAGAAAGGAGAAGTTACCAA+AGG | 0.658788 | 1.3:-4061579 | MS.gene67136:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTAAATAAATCAAAATC+TGG | + | chr1.3:4061161-4061180 | None:intergenic | 10.0% |
| !!! | TATAGATTTTCATTATATTA+AGG | - | chr1.3:4061018-4061037 | MS.gene67136:intron | 10.0% |
| !! | AAATATACTTCATATATGTT+TGG | - | chr1.3:4061337-4061356 | MS.gene67136:intron | 15.0% |
| !! | ATATATGAAGTATATTTGTA+GGG | + | chr1.3:4061334-4061353 | None:intergenic | 15.0% |
| !! | TGAAATAATAAAGAAGAAAA+AGG | + | chr1.3:4060646-4060665 | None:intergenic | 15.0% |
| !!! | TATTTAAAATCTGAAAAAGT+AGG | - | chr1.3:4061171-4061190 | MS.gene67136:intron | 15.0% |
| !! | AAATAATCATACATTAAGGA+TGG | + | chr1.3:4061539-4061558 | None:intergenic | 20.0% |
| !! | AACCAAATAATCATACATTA+AGG | + | chr1.3:4061543-4061562 | None:intergenic | 20.0% |
| !! | ACAAGAAGAGATTATAATTT+AGG | + | chr1.3:4060698-4060717 | None:intergenic | 20.0% |
| !! | AGAAAAATGAAATTAACCTT+TGG | + | chr1.3:4060790-4060809 | None:intergenic | 20.0% |
| !! | ATCCTTAATGTATGATTATT+TGG | - | chr1.3:4061538-4061557 | MS.gene67136:intron | 20.0% |
| !! | ATTATTTGGTTGTGTATATA+GGG | - | chr1.3:4061552-4061571 | MS.gene67136:intron | 20.0% |
| !! | CATATATGAAGTATATTTGT+AGG | + | chr1.3:4061335-4061354 | None:intergenic | 20.0% |
| !! | GCATTTAATCACATAAAATA+TGG | + | chr1.3:4061207-4061226 | None:intergenic | 20.0% |
| !! | TGAATCAAATTGAATTGAAA+AGG | + | chr1.3:4060819-4060838 | None:intergenic | 20.0% |
| !! | TTTAATCACATAAAATATGG+AGG | + | chr1.3:4061204-4061223 | None:intergenic | 20.0% |
| !!! | ATTAAAATTAACCAAGTTGA+TGG | + | chr1.3:4061496-4061515 | None:intergenic | 20.0% |
| !!! | GTTGAAAGTATCTAATATTT+TGG | - | chr1.3:4060887-4060906 | MS.gene67136:intron | 20.0% |
| ! | AAAAAACTCACCGAAAAAAT+TGG | - | chr1.3:4060587-4060606 | MS.gene67136:intron | 25.0% |
| ! | ACAATTATGTTGGCAAAAAT+AGG | - | chr1.3:4061116-4061135 | MS.gene67136:intron | 25.0% |
| ! | AGCAAAATTATAACAGTTGT+TGG | + | chr1.3:4061279-4061298 | None:intergenic | 25.0% |
| ! | ATCCTAAAATCTTGACATTA+TGG | + | chr1.3:4060941-4060960 | None:intergenic | 25.0% |
| ! | CAATTATGTTGGCAAAAATA+GGG | - | chr1.3:4061117-4061136 | MS.gene67136:intron | 25.0% |
| ! | GATTATTTGGTTGTGTATAT+AGG | - | chr1.3:4061551-4061570 | MS.gene67136:intron | 25.0% |
| ! | TAAACAAAGAAAAGCCAAAA+CGG | + | chr1.3:4061718-4061737 | None:intergenic | 25.0% |
| ! | TTATTTGGTTGTGTATATAG+GGG | - | chr1.3:4061553-4061572 | MS.gene67136:intron | 25.0% |
| !! | AACCATAATGTCAAGATTTT+AGG | - | chr1.3:4060936-4060955 | MS.gene67136:intron | 25.0% |
| !! | AAGGTAAAATGATTTGTGTT+TGG | - | chr1.3:4061418-4061437 | MS.gene67136:intron | 25.0% |
| !! | TTGTGAATTTTGCAGAAAAT+TGG | - | chr1.3:4060717-4060736 | MS.gene67136:CDS | 25.0% |
| !!! | ATAAACGAGACCAATTTTTT+CGG | + | chr1.3:4060600-4060619 | None:intergenic | 25.0% |
| !!! | ATTGTTCTGAAATTGCTTTA+AGG | - | chr1.3:4060865-4060884 | MS.gene67136:intron | 25.0% |
| AATAGATACAAGGAAGTGAA+TGG | + | chr1.3:4061742-4061761 | None:intergenic | 30.0% | |
| AATTGTAGTTACTGATGCAT+TGG | - | chr1.3:4060984-4061003 | MS.gene67136:intron | 30.0% | |
| ACTTTGTTAAGGTTGCATTA+GGG | - | chr1.3:4061624-4061643 | MS.gene67136:intron | 30.0% | |
| GTAAATGAAAAAGTTGCATC+TGG | + | chr1.3:4061596-4061615 | None:intergenic | 30.0% | |
| TAAAGAAGAAAAAGGACCTT+AGG | + | chr1.3:4060638-4060657 | None:intergenic | 30.0% | |
| TACAGATGAAGACTTTGTTA+AGG | - | chr1.3:4061613-4061632 | MS.gene67136:CDS | 30.0% | |
| TGGATGAGATACAATTATGT+TGG | - | chr1.3:4061106-4061125 | MS.gene67136:intron | 30.0% | |
| ! | AATTTTGTCCCCTATAAAAG+TGG | - | chr1.3:4061459-4061478 | MS.gene67136:intron | 30.0% |
| ! | TAGATTATAGTTTGTGCTCT+AGG | - | chr1.3:4061239-4061258 | MS.gene67136:intron | 30.0% |
| ! | TATGACTAAAAGTGAGTTGA+AGG | - | chr1.3:4061399-4061418 | MS.gene67136:intron | 30.0% |
| !!! | ATGTTTTGGTCCACTTTTAT+AGG | + | chr1.3:4061472-4061491 | None:intergenic | 30.0% |
| !!! | TGTTCTCTTTTTTTCCGTTT+TGG | - | chr1.3:4061701-4061720 | MS.gene67136:intron | 30.0% |
| !!! | TGTTTTGGTCCACTTTTATA+GGG | + | chr1.3:4061471-4061490 | None:intergenic | 30.0% |
| AAGAAAGGAGAAGTTACCAA+AGG | - | chr1.3:4060771-4060790 | MS.gene67136:CDS | 35.0% | |
| GACTTTGTTAAGGTTGCATT+AGG | - | chr1.3:4061623-4061642 | MS.gene67136:intron | 35.0% | |
| GTTGGCAAAAATAGGGATTT+GGG | - | chr1.3:4061124-4061143 | MS.gene67136:intron | 35.0% | |
| TACATTAAGGATGGCATTGT+AGG | + | chr1.3:4061530-4061549 | None:intergenic | 35.0% | |
| TATACCGTTGATCTCAAGAA+AGG | - | chr1.3:4060756-4060775 | MS.gene67136:CDS | 35.0% | |
| TCACTTCCTTGTATCTATTG+TGG | - | chr1.3:4061743-4061762 | MS.gene67136:CDS | 35.0% | |
| TCAGTTTCAAATTGCTCCTA+AGG | - | chr1.3:4060619-4060638 | MS.gene67136:intron | 35.0% | |
| TGTTGGCAAAAATAGGGATT+TGG | - | chr1.3:4061123-4061142 | MS.gene67136:intron | 35.0% | |
| TTCTCCTTTCTTGAGATCAA+CGG | + | chr1.3:4060763-4060782 | None:intergenic | 35.0% | |
| !! | GTTTTGGTCCACTTTTATAG+GGG | + | chr1.3:4061470-4061489 | None:intergenic | 35.0% |
| AACCTCATGAAAGCAATCTG+TGG | + | chr1.3:4061659-4061678 | None:intergenic | 40.0% | |
| ACACCACCACAATAGATACA+AGG | + | chr1.3:4061752-4061771 | None:intergenic | 40.0% | |
| ACCAAAACATGCCATCAACT+TGG | - | chr1.3:4061482-4061501 | MS.gene67136:intron | 40.0% | |
| ATCCACAGATTGCTTTCATG+AGG | - | chr1.3:4061654-4061673 | MS.gene67136:intron | 40.0% | |
| CTTCCTTGTATCTATTGTGG+TGG | - | chr1.3:4061746-4061765 | MS.gene67136:CDS | 40.0% | |
| TGCTGCTCAGAAATTTACTC+CGG | - | chr1.3:4061812-4061831 | MS.gene67136:CDS | 40.0% | |
| ! | AGGGGTGCAATGAAGATTAA+GGG | - | chr1.3:4061780-4061799 | MS.gene67136:CDS | 40.0% |
| ! | ATATAGGGGCATATGAAGGT+GGG | - | chr1.3:4061567-4061586 | MS.gene67136:intron | 40.0% |
| ! | GTGTATATAGGGGCATATGA+AGG | - | chr1.3:4061563-4061582 | MS.gene67136:intron | 40.0% |
| ! | TATATAGGGGCATATGAAGG+TGG | - | chr1.3:4061566-4061585 | MS.gene67136:intron | 40.0% |
| ! | TGTGGTGGTGTATGAATACA+GGG | - | chr1.3:4061761-4061780 | MS.gene67136:CDS | 40.0% |
| ! | TTGTGGTGGTGTATGAATAC+AGG | - | chr1.3:4061760-4061779 | MS.gene67136:CDS | 40.0% |
| !!! | ACCAAGTTGATGGCATGTTT+TGG | + | chr1.3:4061486-4061505 | None:intergenic | 40.0% |
| ATCTGTGAGCGATTTGTGCA+TGG | - | chr1.3:4061086-4061105 | MS.gene67136:intron | 45.0% | |
| ! | CAGGGGTGCAATGAAGATTA+AGG | - | chr1.3:4061779-4061798 | MS.gene67136:CDS | 45.0% |
| ! | GTGGTGGTGTATGAATACAG+GGG | - | chr1.3:4061762-4061781 | MS.gene67136:CDS | 45.0% |
| ! | TAGTTTGTGCTCTAGGTAGC+TGG | - | chr1.3:4061246-4061265 | MS.gene67136:intron | 45.0% |
| !! | GAAGGCTTTGGGAAGATATC+CGG | + | chr1.3:4061834-4061853 | None:intergenic | 45.0% |
| !!! | GGTGAGTTTTTTGCCAGCGT+CGG | + | chr1.3:4060579-4060598 | None:intergenic | 50.0% |
| ! | CAACACTTTTCCACCGACGC+TGG | - | chr1.3:4060563-4060582 | MS.gene67136:CDS | 55.0% |
| !!! | GAGTTTTTTGCCAGCGTCGG+TGG | + | chr1.3:4060576-4060595 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 4060509 | 4061863 | 4060509 | ID=MS.gene67136 |
| chr1.3 | mRNA | 4060509 | 4061863 | 4060509 | ID=MS.gene67136.t1;Parent=MS.gene67136 |
| chr1.3 | exon | 4061732 | 4061863 | 4061732 | ID=MS.gene67136.t1.exon1;Parent=MS.gene67136.t1 |
| chr1.3 | CDS | 4061732 | 4061863 | 4061732 | ID=cds.MS.gene67136.t1;Parent=MS.gene67136.t1 |
| chr1.3 | exon | 4061580 | 4061640 | 4061580 | ID=MS.gene67136.t1.exon2;Parent=MS.gene67136.t1 |
| chr1.3 | CDS | 4061580 | 4061640 | 4061580 | ID=cds.MS.gene67136.t1;Parent=MS.gene67136.t1 |
| chr1.3 | exon | 4060697 | 4060799 | 4060697 | ID=MS.gene67136.t1.exon3;Parent=MS.gene67136.t1 |
| chr1.3 | CDS | 4060697 | 4060799 | 4060697 | ID=cds.MS.gene67136.t1;Parent=MS.gene67136.t1 |
| chr1.3 | exon | 4060509 | 4060590 | 4060509 | ID=MS.gene67136.t1.exon4;Parent=MS.gene67136.t1 |
| chr1.3 | CDS | 4060509 | 4060590 | 4060509 | ID=cds.MS.gene67136.t1;Parent=MS.gene67136.t1 |
| Gene Sequence |
| Protein sequence |
