AlfalfaGEDB | MS.gene68767

M. sativa cultivar XinJiangDaYe / MS.gene68767

KEGG: K19033
GO: GO:0005575 GO:0005840 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0110165
PFAM: PF17067.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68767.t1	KEH36378.1	95.1	81	3	1	1	81	1	80	3.00E-32	147.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68767.t1	P47909	57.7	71	27	1	11	81	4	71	4.5e-13	75.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68767.t1	A0A072V4Y3	95.1	81	3	1	1	81	1	80	2.1e-32	147.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene68767	0.809706	1.66E-50	-1.69E-46
MS.gene049341	MS.gene68767	0.802518	5.40E-49	-1.69E-46
MS.gene049382	MS.gene68767	0.807067	6.06E-50	-1.69E-46
MS.gene050020	MS.gene68767	0.808766	2.64E-50	-1.69E-46
MS.gene050237	MS.gene68767	0.802808	4.71E-49	-1.69E-46
MS.gene050277	MS.gene68767	0.823268	1.52E-53	-1.69E-46
MS.gene050586	MS.gene68767	0.823661	1.23E-53	-1.69E-46
MS.gene050807	MS.gene68767	0.820622	6.22E-53	-1.69E-46
MS.gene050815	MS.gene68767	0.818278	2.13E-52	-1.69E-46
MS.gene051243	MS.gene68767	0.849121	3.96E-60	-1.69E-46
MS.gene051328	MS.gene68767	0.831595	1.53E-55	-1.69E-46
MS.gene05134	MS.gene68767	0.814918	1.21E-51	-1.69E-46
MS.gene051577	MS.gene68767	0.815012	1.15E-51	-1.69E-46
MS.gene051757	MS.gene68767	0.801228	9.94E-49	-1.69E-46
MS.gene051789	MS.gene68767	0.800789	1.22E-48	-1.69E-46
MS.gene052302	MS.gene68767	0.800306	1.53E-48	-1.69E-46
MS.gene052376	MS.gene68767	0.80935	1.98E-50	-1.69E-46
MS.gene052665	MS.gene68767	0.815543	8.76E-52	-1.69E-46
MS.gene052856	MS.gene68767	0.814955	1.18E-51	-1.69E-46
MS.gene052858	MS.gene68767	0.800904	1.16E-48	-1.69E-46
MS.gene052860	MS.gene68767	0.812817	3.50E-51	-1.69E-46
MS.gene052947	MS.gene68767	0.801373	9.28E-49	-1.69E-46
MS.gene053074	MS.gene68767	0.804764	1.85E-49	-1.69E-46
MS.gene053630	MS.gene68767	0.805554	1.26E-49	-1.69E-46
MS.gene05462	MS.gene68767	0.807331	5.33E-50	-1.69E-46
MS.gene056069	MS.gene68767	-0.815116	1.09E-51	-1.69E-46
MS.gene056580	MS.gene68767	0.807776	4.29E-50	-1.69E-46
MS.gene056760	MS.gene68767	0.843222	1.60E-58	-1.69E-46
MS.gene057057	MS.gene68767	0.807178	5.74E-50	-1.69E-46
MS.gene057140	MS.gene68767	0.807936	3.96E-50	-1.69E-46
MS.gene058906	MS.gene68767	0.838752	2.39E-57	-1.69E-46
MS.gene06001	MS.gene68767	0.821721	3.47E-53	-1.69E-46
MS.gene060480	MS.gene68767	0.806017	1.01E-49	-1.69E-46
MS.gene060803	MS.gene68767	0.806775	6.98E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68767.t1	MTR_3g117570	93.902	82	4	1	1	82	1	81	2.05e-49	152
MS.gene68767.t1	MTR_3g117570	95.062	81	3	1	1	81	1	80	6.40e-49	151

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 26 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCACAAATACAGATTGTT+TGG	0.302653	3.2:-92817785	MS.gene68767:intron
ATAACTACTTCTCTTGGTTA+TGG	0.376607	3.2:-92817725	MS.gene68767:CDS
TCGATCATTTGTTCTCTCTT+CGG	0.404252	3.2:+92818458	None:intergenic
GCTGCACGGTGGTTGATTAG+AGG	0.449554	3.2:-92818605	MS.gene68767:CDS
AAAAGATTCAAGGGCTCGTA+TGG	0.464506	3.2:-92818491	MS.gene68767:CDS
AAAGAAAGGGAAAAGATTCA+AGG	0.481880	3.2:-92818501	MS.gene68767:CDS
ACGCCTTGTGCTTCTTCTGC+CGG	0.494602	3.2:-92818569	MS.gene68767:CDS
GAGTGCATAACTACTTCTCT+TGG	0.501421	3.2:-92817731	MS.gene68767:CDS
GATCGAACGAATCAAAGATA+AGG	0.533385	3.2:-92818441	MS.gene68767:CDS
GCTCCGATAGTGTGCGGACG+CGG	0.535798	3.2:-92818536	MS.gene68767:CDS
TCACCGCGTCCGCACACTAT+CGG	0.538358	3.2:+92818533	None:intergenic
AATCAACCACCGTGCAGCTA+CGG	0.543022	3.2:+92818610	None:intergenic
AAGATAAGGTTGAAGTTCCC+AGG	0.546325	3.2:-92818427	MS.gene68767:intron
AGGAATTTCGATAAAAGAAG+TGG	0.550885	3.2:-92817702	MS.gene68767:CDS
TATCGGAGCAGCTGTTACGC+CGG	0.576176	3.2:+92818550	None:intergenic
AAGAAAGGGAAAAGATTCAA+GGG	0.576778	3.2:-92818500	MS.gene68767:CDS
ACTACTTCTCTTGGTTATGG+AGG	0.586505	3.2:-92817722	MS.gene68767:CDS
AGCGGTGCACCGTAGCTGCA+CGG	0.586905	3.2:-92818619	MS.gene68767:CDS
GTTGATTAGAGGAGCGTCGT+CGG	0.603720	3.2:-92818594	MS.gene68767:CDS
AAAGATTCAAGGGCTCGTAT+GGG	0.635720	3.2:-92818490	MS.gene68767:CDS
GTGGCGCTAGTGTGATGCAG+CGG	0.656151	3.2:-92818637	MS.gene68767:CDS
AAAGTGTGTATGGCGAGTGG+TGG	0.695087	3.2:-92818656	None:intergenic
ACAGCTGCTCCGATAGTGTG+CGG	0.696198	3.2:-92818542	MS.gene68767:CDS
AGAAAAGTGTGTATGGCGAG+TGG	0.701894	3.2:-92818659	None:intergenic
ACGCCGGCAGAAGAAGCACA+AGG	0.711011	3.2:+92818566	None:intergenic
GGTGCACCGTAGCTGCACGG+TGG	0.772757	3.2:-92818616	MS.gene68767:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTAAAATCAAAATCCATA+AGG	-	chr3.2:92818263-92818282	MS.gene68767:intron	15.0%
!!!	TTTTCTATTGAAATAGAAAA+TGG	+	chr3.2:92818020-92818039	None:intergenic	15.0%
!!	GAAATTGTTCATAGATATAT+AGG	-	chr3.2:92818448-92818467	MS.gene68767:CDS	20.0%
!!	TTTGATGCATTATATTATGT+TGG	-	chr3.2:92818046-92818065	MS.gene68767:intron	20.0%
!!!	TATTTTGAGAATACTACTAA+TGG	-	chr3.2:92818350-92818369	MS.gene68767:intron	20.0%
!	GTGATAAAAAGACAAAGAAA+GGG	-	chr3.2:92817812-92817831	MS.gene68767:intron	25.0%
!	TAAATTTGTGACTTACAAGT+TGG	+	chr3.2:92818295-92818314	None:intergenic	25.0%
!!	AAGTATTTGAAGTGAATTAG+AGG	+	chr3.2:92818233-92818252	None:intergenic	25.0%
!!	AGTATTTGAAGTGAATTAGA+GGG	+	chr3.2:92818232-92818251	None:intergenic	25.0%
!!	TCTTGCAAATTTTCCAAAAT+GGG	-	chr3.2:92818396-92818415	MS.gene68767:intron	25.0%
!!!	TCATTTCATTCCCATTTTTA+GGG	-	chr3.2:92817960-92817979	MS.gene68767:intron	25.0%
!!!	TTCATTTCATTCCCATTTTT+AGG	-	chr3.2:92817959-92817978	MS.gene68767:intron	25.0%
	AAACAATCTGTATTTGTGAG+TGG	+	chr3.2:92818542-92818561	None:intergenic	30.0%
	AAAGAAAGGGAAAAGATTCA+AGG	-	chr3.2:92817825-92817844	MS.gene68767:intron	30.0%
	AAATGCTAGTAATGTTTGTG+GGG	+	chr3.2:92818322-92818341	None:intergenic	30.0%
	AAGAAAGGGAAAAGATTCAA+GGG	-	chr3.2:92817826-92817845	MS.gene68767:intron	30.0%
	ACTCACAAATACAGATTGTT+TGG	-	chr3.2:92818541-92818560	MS.gene68767:CDS	30.0%
	AGAAATGCTAGTAATGTTTG+TGG	+	chr3.2:92818324-92818343	None:intergenic	30.0%
	AGGAATTTCGATAAAAGAAG+TGG	-	chr3.2:92818624-92818643	MS.gene68767:CDS	30.0%
	ATAACTACTTCTCTTGGTTA+TGG	-	chr3.2:92818601-92818620	MS.gene68767:CDS	30.0%
	GAAATGCTAGTAATGTTTGT+GGG	+	chr3.2:92818323-92818342	None:intergenic	30.0%
	GGTGATAAAAAGACAAAGAA+AGG	-	chr3.2:92817811-92817830	MS.gene68767:intron	30.0%
	TCTTTACTCAAAGTTAGTGA+GGG	-	chr3.2:92818493-92818512	MS.gene68767:CDS	30.0%
	TGAAATCAAATGAGCTTGAA+AGG	+	chr3.2:92817943-92817962	None:intergenic	30.0%
!	AAAATGGCAATGAAAGTACA+TGG	+	chr3.2:92818004-92818023	None:intergenic	30.0%
!	GTCTTGCAAATTTTCCAAAA+TGG	-	chr3.2:92818395-92818414	MS.gene68767:intron	30.0%
!!!	GACTTTATTTGTTTTGTGAG+TGG	+	chr3.2:92818378-92818397	None:intergenic	30.0%
	AATTCCACAGTGTTGTGTTT+GGG	-	chr3.2:92818080-92818099	MS.gene68767:intron	35.0%
	AGACGAGAAAACCCTAAAAA+TGG	+	chr3.2:92817974-92817993	None:intergenic	35.0%
	ATATAGGACACACAGATTCA+TGG	-	chr3.2:92818464-92818483	MS.gene68767:CDS	35.0%
	CTTTACTCAAAGTTAGTGAG+GGG	-	chr3.2:92818494-92818513	MS.gene68767:CDS	35.0%
	GACGAGAAAACCCTAAAAAT+GGG	+	chr3.2:92817973-92817992	None:intergenic	35.0%
	GATCGAACGAATCAAAGATA+AGG	-	chr3.2:92817885-92817904	MS.gene68767:intron	35.0%
	GTCTTTACTCAAAGTTAGTG+AGG	-	chr3.2:92818492-92818511	MS.gene68767:CDS	35.0%
	TAATTCCACAGTGTTGTGTT+TGG	-	chr3.2:92818079-92818098	MS.gene68767:intron	35.0%
	TCGATCATTTGTTCTCTCTT+CGG	+	chr3.2:92817871-92817890	None:intergenic	35.0%
	TTTACTCAAAGTTAGTGAGG+GGG	-	chr3.2:92818495-92818514	MS.gene68767:CDS	35.0%
!	GTAAAATAGCATGCCCATTT+TGG	+	chr3.2:92818412-92818431	None:intergenic	35.0%
!!	ACAAGTTGGTTTCAACCTTA+TGG	+	chr3.2:92818281-92818300	None:intergenic	35.0%
!!!	AAATGGGCATGCTATTTTAC+TGG	-	chr3.2:92818412-92818431	MS.gene68767:intron	35.0%
!!!	AATGGGCATGCTATTTTACT+GGG	-	chr3.2:92818413-92818432	MS.gene68767:intron	35.0%
	AAAACCCAAACACAACACTG+TGG	+	chr3.2:92818087-92818106	None:intergenic	40.0%
	AAAAGATTCAAGGGCTCGTA+TGG	-	chr3.2:92817835-92817854	MS.gene68767:intron	40.0%
	AAAGATTCAAGGGCTCGTAT+GGG	-	chr3.2:92817836-92817855	MS.gene68767:intron	40.0%
	AAGATAAGGTTGAAGTTCCC+AGG	-	chr3.2:92817899-92817918	MS.gene68767:intron	40.0%
	ACTACTTCTCTTGGTTATGG+AGG	-	chr3.2:92818604-92818623	MS.gene68767:CDS	40.0%
	CAAATGAGCTTGAAAGGAAG+AGG	+	chr3.2:92817937-92817956	None:intergenic	40.0%
	GAGTGCATAACTACTTCTCT+TGG	-	chr3.2:92818595-92818614	MS.gene68767:CDS	40.0%
	AAGTTCCCAGGTCTACTCCT+TGG	-	chr3.2:92817911-92817930	MS.gene68767:intron	50.0%
	AATCAACCACCGTGCAGCTA+CGG	+	chr3.2:92817719-92817738	None:intergenic	50.0%
	AGCTTGAAAGGAAGAGGCCA+AGG	+	chr3.2:92817931-92817950	None:intergenic	50.0%
	GGGGTCATTCTTGGTTGATC+TGG	-	chr3.2:92818514-92818533	MS.gene68767:CDS	50.0%
	GTTAGTGAGGGGGTCATTCT+TGG	-	chr3.2:92818505-92818524	MS.gene68767:CDS	50.0%
!!	GTTGATTAGAGGAGCGTCGT+CGG	-	chr3.2:92817732-92817751	MS.gene68767:CDS	50.0%
	AAGAGGCCAAGGAGTAGACC+TGG	+	chr3.2:92817920-92817939	None:intergenic	55.0%
	ACAGCTGCTCCGATAGTGTG+CGG	-	chr3.2:92817784-92817803	MS.gene68767:intron	55.0%
	ACGCCTTGTGCTTCTTCTGC+CGG	-	chr3.2:92817757-92817776	MS.gene68767:CDS	55.0%
	AGAGGCCAAGGAGTAGACCT+GGG	+	chr3.2:92817919-92817938	None:intergenic	55.0%
	GCTGCACGGTGGTTGATTAG+AGG	-	chr3.2:92817721-92817740	MS.gene68767:CDS	55.0%
	TATCGGAGCAGCTGTTACGC+CGG	+	chr3.2:92817779-92817798	None:intergenic	55.0%
	GTGGCGCTAGTGTGATGCAG+CGG	-	chr3.2:92817689-92817708	MS.gene68767:CDS	60.0%
	TCACCGCGTCCGCACACTAT+CGG	+	chr3.2:92817796-92817815	None:intergenic	60.0%
!	ACGCCGGCAGAAGAAGCACA+AGG	+	chr3.2:92817763-92817782	None:intergenic	60.0%
	AGCGGTGCACCGTAGCTGCA+CGG	-	chr3.2:92817707-92817726	MS.gene68767:CDS	65.0%
	GCTCCGATAGTGTGCGGACG+CGG	-	chr3.2:92817790-92817809	MS.gene68767:intron	65.0%
	GGTGCACCGTAGCTGCACGG+TGG	-	chr3.2:92817710-92817729	MS.gene68767:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	92817679	92818669	92817679	ID=MS.gene68767
chr3.2	mRNA	92817679	92818669	92817679	ID=MS.gene68767.t1;Parent=MS.gene68767
chr3.2	exon	92818428	92818669	92818428	ID=MS.gene68767.t1.exon1;Parent=MS.gene68767.t1
chr3.2	CDS	92818428	92818669	92818428	ID=cds.MS.gene68767.t1;Parent=MS.gene68767.t1
chr3.2	exon	92817679	92817793	92817679	ID=MS.gene68767.t1.exon2;Parent=MS.gene68767.t1
chr3.2	CDS	92817679	92817793	92817679	ID=cds.MS.gene68767.t1;Parent=MS.gene68767.t1

Gene Sequence

>MS.gene68767

ATGGCGAGTGGTGGCGCTAGTGTGATGCAGCGGTGCACCGTAGCTGCACGGTGGTTGATTAGAGGAGCGTCGTCGGAGACGCCTTGTGCTTCTTCTGCCGGCGTAACAGCTGCTCCGATAGTGTGCGGACGCGGTGATAAAAAGACAAAGAAAGGGAAAAGATTCAAGGGCTCGTATGGGAAATCTCGTCCGAAGAGAGAACAAATGATCGAACGAATCAAAGATAAGGTTGAAGTTCCCAGGTCTACTCCTTGGCCTCTTCCTTTCAAGCTCATTTGATTTCATTTCATTCCCATTTTTAGGGTTTTCTCGTCTGAACAATCCATGTACTTTCATTGCCATTTTCTATTTCAATAGAAAATTTTATTTTGATGCATTATATTATGTTGGTTGCTCATTGTAATTCCACAGTGTTGTGTTTGGGTTTTATCAATCATTACAAACAAATTAATAAATTTGCTGTGCAATATTGTTGTTTATGCTAATAGATTAATCATTTCACTCTCACAGACACTTGTATAGAAAAATATCGTAAATATGAAAAGTAAAGCCCTCTAATTCACTTCAAATACTTAATCAGATTGATTTAAAATCAAAATCCATAAGGTTGAAACCAACTTGTAAGTCACAAATTTAAGAGCCCCACAAACATTACTAGCATTTCTAGAAAATATTTTGAGAATACTACTAATGGAACCACTCACAAAACAAATAAAGTCTTGCAAATTTTCCAAAATGGGCATGCTATTTTACTGGGATTCGTTTTTTAGAAATTGTTCATAGATATATAGGACACACAGATTCATGGACACAGTCTTTACTCAAAGTTAGTGAGGGGGTCATTCTTGGTTGATCTGGTTCCACTCACAAATACAGATTGTTTGGAATGTGCACTGCAGCATTTGCAGAGAAATTAGAGTGCATAACTACTTCTCTTGGTTATGGAGGAATTTCGATAAAAGAAGTGGACAGTTCTTGTATAAAATGCTAA

Protein sequence

>MS.gene68767.t1

MASGGASVMQRCTVAARWLIRGASSETPCASSAGVTAAPIVCGRGDKKTKKGKRFKGSYGKSRPKREQMIERIKDKVEVPRLFGMCTAAFAEKLECITTSLGYGGISIKEVDSSCIKC