Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68840.t1 | KEH27276.1 | 98.2 | 56 | 1 | 0 | 1 | 56 | 1 | 56 | 1.60E-23 | 118.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68840.t1 | A0A072UCT8 | 98.2 | 56 | 1 | 0 | 1 | 56 | 1 | 56 | 1.1e-23 | 118.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049341 | MS.gene68840 | 0.816662 | 4.93E-52 | -1.69E-46 |
| MS.gene049703 | MS.gene68840 | -0.831869 | 1.31E-55 | -1.69E-46 |
| MS.gene04984 | MS.gene68840 | 0.802319 | 5.93E-49 | -1.69E-46 |
| MS.gene050277 | MS.gene68840 | 0.840174 | 1.02E-57 | -1.69E-46 |
| MS.gene051245 | MS.gene68840 | 0.845851 | 3.14E-59 | -1.69E-46 |
| MS.gene051328 | MS.gene68840 | 0.82269 | 2.07E-53 | -1.69E-46 |
| MS.gene051577 | MS.gene68840 | 0.80053 | 1.38E-48 | -1.69E-46 |
| MS.gene051633 | MS.gene68840 | 0.813511 | 2.47E-51 | -1.69E-46 |
| MS.gene051746 | MS.gene68840 | 0.828244 | 1.00E-54 | -1.69E-46 |
| MS.gene051748 | MS.gene68840 | 0.821635 | 3.64E-53 | -1.69E-46 |
| MS.gene051757 | MS.gene68840 | 0.818932 | 1.51E-52 | -1.69E-46 |
| MS.gene052286 | MS.gene68840 | 0.800186 | 1.62E-48 | -1.69E-46 |
| MS.gene052302 | MS.gene68840 | 0.810943 | 8.96E-51 | -1.69E-46 |
| MS.gene052376 | MS.gene68840 | 0.801535 | 8.60E-49 | -1.69E-46 |
| MS.gene052856 | MS.gene68840 | 0.810151 | 1.33E-50 | -1.69E-46 |
| MS.gene053109 | MS.gene68840 | 0.814514 | 1.48E-51 | -1.69E-46 |
| MS.gene055441 | MS.gene68840 | 0.815703 | 8.07E-52 | -1.69E-46 |
| MS.gene056432 | MS.gene68840 | 0.811084 | 8.35E-51 | -1.69E-46 |
| MS.gene056760 | MS.gene68840 | 0.812098 | 5.03E-51 | -1.69E-46 |
| MS.gene057057 | MS.gene68840 | 0.872508 | 3.01E-67 | -1.69E-46 |
| MS.gene057094 | MS.gene68840 | 0.802752 | 4.83E-49 | -1.69E-46 |
| MS.gene057140 | MS.gene68840 | 0.821359 | 4.21E-53 | -1.69E-46 |
| MS.gene057234 | MS.gene68840 | 0.856518 | 3.05E-62 | -1.69E-46 |
| MS.gene058054 | MS.gene68840 | 0.839252 | 1.77E-57 | -1.69E-46 |
| MS.gene058055 | MS.gene68840 | 0.871357 | 7.27E-67 | -1.69E-46 |
| MS.gene058182 | MS.gene68840 | 0.806339 | 8.63E-50 | -1.69E-46 |
| MS.gene058238 | MS.gene68840 | 0.843823 | 1.11E-58 | -1.69E-46 |
| MS.gene058646 | MS.gene68840 | 0.806167 | 9.38E-50 | -1.69E-46 |
| MS.gene058906 | MS.gene68840 | 0.84793 | 8.47E-60 | -1.69E-46 |
| MS.gene059898 | MS.gene68840 | 0.806181 | 9.32E-50 | -1.69E-46 |
| MS.gene060165 | MS.gene68840 | 0.804704 | 1.90E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68840.t1 | MTR_6g093010 | 84.848 | 66 | 10 | 0 | 1 | 66 | 1 | 66 | 6.99e-36 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68840.t1 | AT2G31490 | 73.770 | 61 | 16 | 0 | 1 | 61 | 1 | 61 | 2.36e-30 | 102 |
Find 17 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTCGCAATCTTCTTCTTGT+TGG | 0.279849 | 6.1:+79840073 | MS.gene68840:CDS |
| CTTCTTCTTGTTGGTATCTT+CGG | 0.319314 | 6.1:+79840082 | MS.gene68840:CDS |
| AAACAAATACATTGAAGATT+GGG | 0.354720 | 6.1:+79840009 | MS.gene68840:CDS |
| TTGCGATGTTGGAGCATAGA+TGG | 0.385992 | 6.1:+79841805 | MS.gene68840:CDS |
| AAAACAAATACATTGAAGAT+TGG | 0.399828 | 6.1:+79840008 | MS.gene68840:CDS |
| ATGCCTTTGTAGACGAGAAC+AGG | 0.444933 | 6.1:-79840115 | None:intergenic |
| ATCGAAAATGGGTGGAGGAA+TGG | 0.519119 | 6.1:+79839976 | None:intergenic |
| GAATTTAAAGTTTGCGATGT+TGG | 0.525831 | 6.1:+79841794 | MS.gene68840:intron |
| CCAATTTACTACAGCTAACA+AGG | 0.533803 | 6.1:-79841849 | None:intergenic |
| ATCTCGAACACAATTTCCGT+TGG | 0.545751 | 6.1:+79840047 | MS.gene68840:CDS |
| TGCGATGTTGGAGCATAGAT+GGG | 0.574819 | 6.1:+79841806 | MS.gene68840:CDS |
| GAAGATTGCGACGAGACCAA+CGG | 0.577698 | 6.1:-79840063 | None:intergenic |
| TCATCATCGAAAATGGGTGG+AGG | 0.588259 | 6.1:+79839971 | None:intergenic |
| AACAAATACATTGAAGATTG+GGG | 0.598822 | 6.1:+79840010 | MS.gene68840:CDS |
| GTTCCTGTTCTCGTCTACAA+AGG | 0.608218 | 6.1:+79840112 | MS.gene68840:CDS |
| TCATCATCATCGAAAATGGG+TGG | 0.630058 | 6.1:+79839968 | None:intergenic |
| AACAAGGTCTGTAGACAGTG+CGG | 0.705055 | 6.1:-79841833 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACTTATTTTCAATTGTTTT+GGG | + | chr6.1:79840426-79840445 | MS.gene68840:intron | 15.0% |
| !!! | TGATTAAAGTTGAATTTTTT+GGG | + | chr6.1:79840288-79840307 | MS.gene68840:intron | 15.0% |
| !!! | TGTTTTATTATAAGCTATTT+TGG | + | chr6.1:79841258-79841277 | MS.gene68840:intron | 15.0% |
| !!! | TTGATTAAAGTTGAATTTTT+TGG | + | chr6.1:79840287-79840306 | MS.gene68840:intron | 15.0% |
| !! | AAAAAATAAGTCAATCCAAA+AGG | - | chr6.1:79840453-79840472 | None:intergenic | 20.0% |
| !! | AAAACAAATACATTGAAGAT+TGG | + | chr6.1:79840008-79840027 | MS.gene68840:CDS | 20.0% |
| !! | AAACAAATACATTGAAGATT+GGG | + | chr6.1:79840009-79840028 | MS.gene68840:CDS | 20.0% |
| !! | ATTTCTGAATAGTTCAATTT+AGG | + | chr6.1:79840197-79840216 | MS.gene68840:intron | 20.0% |
| !! | GAAAAATTGTGTTTATCTAA+GGG | - | chr6.1:79840633-79840652 | None:intergenic | 20.0% |
| !! | TATTAACAACTCAGAATTTA+GGG | + | chr6.1:79840876-79840895 | MS.gene68840:intron | 20.0% |
| !! | TGAAAAATTGTGTTTATCTA+AGG | - | chr6.1:79840634-79840653 | None:intergenic | 20.0% |
| !! | TTATTAACAACTCAGAATTT+AGG | + | chr6.1:79840875-79840894 | MS.gene68840:intron | 20.0% |
| !!! | AACACATTTTAGCATTATTT+TGG | + | chr6.1:79840685-79840704 | MS.gene68840:intron | 20.0% |
| !!! | ACACATTTTAGCATTATTTT+GGG | + | chr6.1:79840686-79840705 | MS.gene68840:intron | 20.0% |
| !!! | CAACTTATTTTCAATTGTTT+TGG | + | chr6.1:79840425-79840444 | MS.gene68840:intron | 20.0% |
| !!! | CAATTTTTACAAGCTTTTTT+CGG | - | chr6.1:79841302-79841321 | None:intergenic | 20.0% |
| ! | AACAAATACATTGAAGATTG+GGG | + | chr6.1:79840010-79840029 | MS.gene68840:CDS | 25.0% |
| ! | AACAGTTCAAAAGAATTTAG+GGG | + | chr6.1:79840934-79840953 | MS.gene68840:intron | 25.0% |
| ! | ACAACAGTTCAAAAGAATTT+AGG | + | chr6.1:79840932-79840951 | MS.gene68840:intron | 25.0% |
| ! | AGTTCAATTTAGGAATTTGA+GGG | + | chr6.1:79840207-79840226 | MS.gene68840:intron | 25.0% |
| ! | CAACAGTTCAAAAGAATTTA+GGG | + | chr6.1:79840933-79840952 | MS.gene68840:intron | 25.0% |
| ! | CAATCTAAACAAATTGAGTA+TGG | + | chr6.1:79841645-79841664 | MS.gene68840:intron | 25.0% |
| ! | CAGAATTTAGGGATTTAATT+AGG | + | chr6.1:79840887-79840906 | MS.gene68840:intron | 25.0% |
| ! | GAGTTATTTGAGCTTATATA+TGG | + | chr6.1:79840331-79840350 | MS.gene68840:intron | 25.0% |
| ! | GTTGTTAATAAATTGACCAA+TGG | - | chr6.1:79840866-79840885 | None:intergenic | 25.0% |
| ! | TAAATGCTTGCTTGAATTAT+TGG | + | chr6.1:79841696-79841715 | MS.gene68840:intron | 25.0% |
| ! | TAGTTCAATTTAGGAATTTG+AGG | + | chr6.1:79840206-79840225 | MS.gene68840:intron | 25.0% |
| ! | TGATTATTTCAATCTACCAT+TGG | + | chr6.1:79840847-79840866 | MS.gene68840:intron | 25.0% |
| ! | TGGTTGTAATGTTTAAATTG+TGG | + | chr6.1:79841716-79841735 | MS.gene68840:intron | 25.0% |
| ! | TTAATGCTTGCTTGAATTAT+TGG | + | chr6.1:79841753-79841772 | MS.gene68840:intron | 25.0% |
| !!! | AAAGTTTCTTACTTTTTCTG+TGG | + | chr6.1:79840253-79840272 | MS.gene68840:intron | 25.0% |
| !!! | AAGAATTTAGGGGTTTTATT+AGG | + | chr6.1:79840944-79840963 | MS.gene68840:intron | 25.0% |
| !!! | AAGTTTCTTACTTTTTCTGT+GGG | + | chr6.1:79840254-79840273 | MS.gene68840:intron | 25.0% |
| !!! | AGTTGAATTTTTTGGGAATA+TGG | + | chr6.1:79840295-79840314 | MS.gene68840:intron | 25.0% |
| !!! | GTTGAATTTTTTGGGAATAT+GGG | + | chr6.1:79840296-79840315 | MS.gene68840:intron | 25.0% |
| AAAAATAAGCCAATCCAAAC+TGG | + | chr6.1:79840607-79840626 | MS.gene68840:intron | 30.0% | |
| AAGAGAGAAGAAGATGAAAA+TGG | - | chr6.1:79840165-79840184 | None:intergenic | 30.0% | |
| ATCCAAACAAGTCCTAAAAT+TGG | + | chr6.1:79841582-79841601 | MS.gene68840:intron | 30.0% | |
| CTTACATAAGCTTATAGCAT+GGG | - | chr6.1:79841203-79841222 | None:intergenic | 30.0% | |
| GCAAATAATTAAGCTTGTCA+AGG | + | chr6.1:79840480-79840499 | MS.gene68840:intron | 30.0% | |
| ! | AAAATTGGATTTCCAGCTTT+TGG | + | chr6.1:79841597-79841616 | MS.gene68840:intron | 30.0% |
| ! | AAAGCTGGAAATCCAATTTT+AGG | - | chr6.1:79841597-79841616 | None:intergenic | 30.0% |
| ! | GAATTTAAAGTTTGCGATGT+TGG | + | chr6.1:79841794-79841813 | MS.gene68840:intron | 30.0% |
| !! | ATAAGTTTTGTGAGCTTGAT+TGG | + | chr6.1:79840356-79840375 | MS.gene68840:intron | 30.0% |
| !! | ATTTTGATCAAGTCTGTGTT+TGG | + | chr6.1:79841126-79841145 | MS.gene68840:intron | 30.0% |
| !! | TTGTTATGAATGCATTGACT+TGG | + | chr6.1:79841095-79841114 | MS.gene68840:intron | 30.0% |
| !!! | ATCCAATTTTAGGACTTGTT+TGG | - | chr6.1:79841587-79841606 | None:intergenic | 30.0% |
| CTTCTTCTTGTTGGTATCTT+CGG | + | chr6.1:79840082-79840101 | MS.gene68840:CDS | 35.0% | |
| GAAGAAGATGAAAATGGAAG+TGG | - | chr6.1:79840159-79840178 | None:intergenic | 35.0% | |
| GCTTACATAAGCTTATAGCA+TGG | - | chr6.1:79841204-79841223 | None:intergenic | 35.0% | |
| TCAAATAAGCCAATCCAAAC+AGG | + | chr6.1:79841384-79841403 | MS.gene68840:intron | 35.0% | |
| TCAATTTAGGAATTTGAGGG+AGG | + | chr6.1:79840210-79840229 | MS.gene68840:intron | 35.0% | |
| !! | AACTGTATTTTGAGCCTGTT+TGG | - | chr6.1:79841401-79841420 | None:intergenic | 35.0% |
| !! | TATTTTGAGCCTGTTTGGAT+TGG | - | chr6.1:79841396-79841415 | None:intergenic | 35.0% |
| !!! | AGCATTATTTTGGGTCTGTT+AGG | + | chr6.1:79840695-79840714 | MS.gene68840:intron | 35.0% |
| !!! | TCAATTGTTTTGGGACCTTT+TGG | + | chr6.1:79840435-79840454 | MS.gene68840:intron | 35.0% |
| ATCTCGAACACAATTTCCGT+TGG | + | chr6.1:79840047-79840066 | MS.gene68840:CDS | 40.0% | |
| CTACTAACATAAGCACCTGT+GGG | - | chr6.1:79841544-79841563 | None:intergenic | 40.0% | |
| GCTTATTTGAGCTTACCAAC+TGG | - | chr6.1:79841374-79841393 | None:intergenic | 40.0% | |
| TCTACTAACATAAGCACCTG+TGG | - | chr6.1:79841545-79841564 | None:intergenic | 40.0% | |
| TGTTTATCTAAGGGCCAGTT+TGG | - | chr6.1:79840624-79840643 | None:intergenic | 40.0% | |
| TTGGGTCTGTTAGGATAAAC+AGG | + | chr6.1:79840704-79840723 | MS.gene68840:intron | 40.0% | |
| TTTGCACCTTATAGCACAAG+CGG | + | chr6.1:79841162-79841181 | MS.gene68840:intron | 40.0% | |
| ! | CATTAACCGATTCCAAAAGC+TGG | - | chr6.1:79841612-79841631 | None:intergenic | 40.0% |
| !! | TTTGGGAATATGGGCTAGTT+TGG | + | chr6.1:79840305-79840324 | MS.gene68840:intron | 40.0% |
| !!! | GGATTTCCAGCTTTTGGAAT+CGG | + | chr6.1:79841603-79841622 | MS.gene68840:intron | 40.0% |
| AACAAGGTCTGTAGACAGTG+CGG | - | chr6.1:79841836-79841855 | None:intergenic | 45.0% | |
| ATCTAAGGGCCAGTTTGGAT+TGG | - | chr6.1:79840619-79840638 | None:intergenic | 45.0% | |
| ATGCCTTTGTAGACGAGAAC+AGG | - | chr6.1:79840118-79840137 | None:intergenic | 45.0% | |
| CGTCGCAATCTTCTTCTTGT+TGG | + | chr6.1:79840073-79840092 | MS.gene68840:CDS | 45.0% | |
| CTCATGACGCAACATTGTGA+TGG | - | chr6.1:79841016-79841035 | None:intergenic | 45.0% | |
| GGAGGTTCTACTACGTGATT+TGG | + | chr6.1:79840228-79840247 | MS.gene68840:intron | 45.0% | |
| GTTCCTGTTCTCGTCTACAA+AGG | + | chr6.1:79840112-79840131 | MS.gene68840:CDS | 45.0% | |
| TGCGATGTTGGAGCATAGAT+GGG | + | chr6.1:79841806-79841825 | MS.gene68840:CDS | 45.0% | |
| TTGCGATGTTGGAGCATAGA+TGG | + | chr6.1:79841805-79841824 | MS.gene68840:CDS | 45.0% | |
| AGTTCTCCAAACAGTCCCAC+AGG | + | chr6.1:79841526-79841545 | MS.gene68840:intron | 50.0% | |
| CGACAACCGCTTGTGCTATA+AGG | - | chr6.1:79841171-79841190 | None:intergenic | 50.0% | |
| CTCACAAGACTTGTGCCAGT+TGG | + | chr6.1:79841356-79841375 | MS.gene68840:intron | 50.0% | |
| GAAGATTGCGACGAGACCAA+CGG | - | chr6.1:79840066-79840085 | None:intergenic | 50.0% | |
| TAAGCACCTGTGGGACTGTT+TGG | - | chr6.1:79841535-79841554 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 79839983 | 79841857 | 79839983 | ID=MS.gene68840 |
| chr6.1 | mRNA | 79839983 | 79841857 | 79839983 | ID=MS.gene68840.t1;Parent=MS.gene68840 |
| chr6.1 | exon | 79839983 | 79840150 | 79839983 | ID=MS.gene68840.t1.exon1;Parent=MS.gene68840.t1 |
| chr6.1 | CDS | 79839983 | 79840150 | 79839983 | ID=cds.MS.gene68840.t1;Parent=MS.gene68840.t1 |
| chr6.1 | exon | 79841804 | 79841857 | 79841804 | ID=MS.gene68840.t1.exon2;Parent=MS.gene68840.t1 |
| chr6.1 | CDS | 79841804 | 79841857 | 79841804 | ID=cds.MS.gene68840.t1;Parent=MS.gene68840.t1 |
| Gene Sequence |
| Protein sequence |
