Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70093.t1 | XP_003630571.1 | 97.1 | 139 | 4 | 0 | 1 | 139 | 1 | 139 | 2.40E-57 | 231.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70093.t1 | P52414 | 55.3 | 114 | 49 | 1 | 27 | 138 | 24 | 137 | 2.3e-24 | 113.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70093.t1 | G7LIR8 | 97.1 | 139 | 4 | 0 | 1 | 139 | 1 | 139 | 1.7e-57 | 231.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049491 | MS.gene70093 | 0.831163 | 1.95E-55 | -1.69E-46 |
| MS.gene049614 | MS.gene70093 | 0.814444 | 1.54E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene70093 | 0.80213 | 6.49E-49 | -1.69E-46 |
| MS.gene050076 | MS.gene70093 | 0.833779 | 4.38E-56 | -1.69E-46 |
| MS.gene050301 | MS.gene70093 | 0.830176 | 3.40E-55 | -1.69E-46 |
| MS.gene050767 | MS.gene70093 | 0.861141 | 1.26E-63 | -1.69E-46 |
| MS.gene050770 | MS.gene70093 | 0.86095 | 1.44E-63 | -1.69E-46 |
| MS.gene05101 | MS.gene70093 | 0.856153 | 3.90E-62 | -1.69E-46 |
| MS.gene051471 | MS.gene70093 | 0.844602 | 6.83E-59 | -1.69E-46 |
| MS.gene052078 | MS.gene70093 | 0.838433 | 2.88E-57 | -1.69E-46 |
| MS.gene052290 | MS.gene70093 | 0.827333 | 1.66E-54 | -1.69E-46 |
| MS.gene052293 | MS.gene70093 | 0.803131 | 4.03E-49 | -1.69E-46 |
| MS.gene052326 | MS.gene70093 | 0.882453 | 1.03E-70 | -1.69E-46 |
| MS.gene053322 | MS.gene70093 | 0.818692 | 1.72E-52 | -1.69E-46 |
| MS.gene054376 | MS.gene70093 | 0.864332 | 1.31E-64 | -1.69E-46 |
| MS.gene05455 | MS.gene70093 | 0.808821 | 2.57E-50 | -1.69E-46 |
| MS.gene054802 | MS.gene70093 | 0.843134 | 1.69E-58 | -1.69E-46 |
| MS.gene054803 | MS.gene70093 | 0.845545 | 3.80E-59 | -1.69E-46 |
| MS.gene054804 | MS.gene70093 | 0.845216 | 4.66E-59 | -1.69E-46 |
| MS.gene055254 | MS.gene70093 | 0.850726 | 1.41E-60 | -1.69E-46 |
| MS.gene055277 | MS.gene70093 | 0.835673 | 1.46E-56 | -1.69E-46 |
| MS.gene055390 | MS.gene70093 | 0.841959 | 3.46E-58 | -1.69E-46 |
| MS.gene055397 | MS.gene70093 | 0.832576 | 8.74E-56 | -1.69E-46 |
| MS.gene055422 | MS.gene70093 | 0.849047 | 4.15E-60 | -1.69E-46 |
| MS.gene055428 | MS.gene70093 | 0.804165 | 2.46E-49 | -1.69E-46 |
| MS.gene055499 | MS.gene70093 | 0.847758 | 9.44E-60 | -1.69E-46 |
| MS.gene055896 | MS.gene70093 | 0.835166 | 1.97E-56 | -1.69E-46 |
| MS.gene056931 | MS.gene70093 | 0.800721 | 1.26E-48 | -1.69E-46 |
| MS.gene057123 | MS.gene70093 | 0.816023 | 6.85E-52 | -1.69E-46 |
| MS.gene057129 | MS.gene70093 | 0.818572 | 1.83E-52 | -1.69E-46 |
| MS.gene057295 | MS.gene70093 | 0.804066 | 2.58E-49 | -1.69E-46 |
| MS.gene058293 | MS.gene70093 | 0.802618 | 5.15E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene70093 | 0.878583 | 2.50E-69 | -1.69E-46 |
| MS.gene059228 | MS.gene70093 | 0.821233 | 4.50E-53 | -1.69E-46 |
| MS.gene059326 | MS.gene70093 | 0.820199 | 7.78E-53 | -1.69E-46 |
| MS.gene059597 | MS.gene70093 | 0.825735 | 3.99E-54 | -1.69E-46 |
| MS.gene059960 | MS.gene70093 | 0.827826 | 1.26E-54 | -1.69E-46 |
| MS.gene060005 | MS.gene70093 | 0.813034 | 3.14E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene70093 | 0.811761 | 5.95E-51 | -1.69E-46 |
| MS.gene060559 | MS.gene70093 | 0.807685 | 4.48E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene70093 | 0.82812 | 1.07E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70093.t1 | MTR_8g099920 | 97.122 | 139 | 4 | 0 | 1 | 139 | 1 | 139 | 2.61e-95 | 271 |
| MS.gene70093.t1 | MTR_8g099920 | 95.683 | 139 | 4 | 1 | 1 | 139 | 1 | 137 | 6.45e-92 | 262 |
| MS.gene70093.t1 | MTR_8g099920 | 95.000 | 100 | 5 | 0 | 1 | 100 | 1 | 100 | 6.72e-64 | 191 |
| MS.gene70093.t1 | MTR_2g020360 | 47.552 | 143 | 65 | 4 | 1 | 138 | 1 | 138 | 4.10e-38 | 126 |
| MS.gene70093.t1 | MTR_2g020360 | 46.853 | 143 | 66 | 4 | 1 | 138 | 1 | 138 | 3.05e-36 | 121 |
| MS.gene70093.t1 | MTR_2g020360 | 51.220 | 123 | 52 | 4 | 22 | 138 | 18 | 138 | 5.29e-34 | 115 |
| MS.gene70093.t1 | MTR_4g118750 | 46.535 | 101 | 35 | 3 | 42 | 138 | 37 | 122 | 3.45e-21 | 83.6 |
| MS.gene70093.t1 | MTR_1g081920 | 50.000 | 80 | 35 | 2 | 56 | 133 | 10 | 86 | 7.93e-18 | 73.6 |
| MS.gene70093.t1 | MTR_7g080760 | 45.833 | 72 | 37 | 2 | 61 | 131 | 42 | 112 | 1.73e-11 | 57.8 |
| MS.gene70093.t1 | MTR_7g080390 | 41.772 | 79 | 44 | 2 | 61 | 138 | 53 | 130 | 4.83e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70093.t1 | AT4G25050 | 56.115 | 139 | 52 | 4 | 1 | 138 | 1 | 131 | 5.38e-40 | 131 |
| MS.gene70093.t1 | AT4G25050 | 51.656 | 151 | 52 | 5 | 1 | 138 | 1 | 143 | 1.49e-36 | 122 |
| MS.gene70093.t1 | AT3G05020 | 50.000 | 136 | 52 | 4 | 4 | 138 | 16 | 136 | 2.84e-34 | 116 |
| MS.gene70093.t1 | AT5G27200 | 58.163 | 98 | 38 | 2 | 42 | 138 | 42 | 137 | 1.20e-31 | 110 |
| MS.gene70093.t1 | AT1G54630 | 43.137 | 153 | 53 | 7 | 1 | 138 | 1 | 134 | 2.51e-28 | 101 |
| MS.gene70093.t1 | AT1G54580 | 57.647 | 85 | 34 | 1 | 54 | 138 | 52 | 134 | 1.13e-26 | 97.4 |
| MS.gene70093.t1 | AT1G65290 | 41.772 | 79 | 44 | 2 | 61 | 138 | 49 | 126 | 1.67e-11 | 58.2 |
| MS.gene70093.t1 | AT1G54630 | 53.448 | 58 | 25 | 1 | 54 | 111 | 52 | 107 | 3.12e-11 | 57.0 |
Find 24 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTCTGAAAATTTAGTTTC+TGG | 0.232292 | 4.1:-6659916 | None:intergenic |
| TCTGGGGTAAGCTTGGTTTC+AGG | 0.264840 | 4.1:-6659898 | None:intergenic |
| CATTATCATCCTCAATATTA+AGG | 0.281641 | 4.1:-6660252 | None:intergenic |
| CTCACTTTCTCCACTGTCTC+AGG | 0.295443 | 4.1:-6659844 | None:intergenic |
| TCTTTCAGAACTTCAAGTTT+TGG | 0.316075 | 4.1:+6659655 | MS.gene70093:CDS |
| GTTCTGAAAATTTAGTTTCT+GGG | 0.362213 | 4.1:-6659915 | None:intergenic |
| GGAAATAGTGATGGGTTTAG+AGG | 0.377032 | 4.1:+6660219 | MS.gene70093:CDS |
| TTTAGTTTCTGGGGTAAGCT+TGG | 0.464049 | 4.1:-6659905 | None:intergenic |
| CAAATCAGCTGCTTGTTGAA+TGG | 0.477053 | 4.1:-6660290 | None:intergenic |
| CCTCTTTCAATTTCTCTACA+AGG | 0.482096 | 4.1:+6658918 | MS.gene70093:CDS |
| AGAGTTTAACCTTAATATTG+AGG | 0.511328 | 4.1:+6660243 | MS.gene70093:CDS |
| GAAATTGAAAGAGGGTTGAA+AGG | 0.517599 | 4.1:-6658909 | None:intergenic |
| TTCTGAAAATTTAGTTTCTG+GGG | 0.526211 | 4.1:-6659914 | None:intergenic |
| CCTTGTAGAGAAATTGAAAG+AGG | 0.526756 | 4.1:-6658918 | None:intergenic |
| AGAGGGTTGAAAGGTAAGAG+AGG | 0.538850 | 4.1:-6658900 | None:intergenic |
| AAGAGAGGTGGTTGAAAGAG+AGG | 0.539433 | 4.1:-6658885 | None:intergenic |
| AAACGAGAAGATCTCAAAGA+AGG | 0.566334 | 4.1:-6659712 | None:intergenic |
| GGGTTGAAAGGTAAGAGAGG+TGG | 0.569945 | 4.1:-6658897 | None:intergenic |
| GTGGCAGGTGGAAATAGTGA+TGG | 0.572304 | 4.1:+6660210 | MS.gene70093:intron |
| CTTGTAGAGAAATTGAAAGA+GGG | 0.583035 | 4.1:-6658917 | None:intergenic |
| TCAGGCACAACCTGAGACAG+TGG | 0.583358 | 4.1:+6659834 | MS.gene70093:CDS |
| TTTCTCTACAAGGACTAACC+AGG | 0.601936 | 4.1:+6658928 | MS.gene70093:CDS |
| TGGCAGGTGGAAATAGTGAT+GGG | 0.642802 | 4.1:+6660211 | MS.gene70093:intron |
| TCAAACTTACTGCACAAATG+CGG | 0.672194 | 4.1:-6659735 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTTTTTTAAAAGATTC+AGG | + | chr4.1:6659816-6659835 | MS.gene70093:intron | 10.0% |
| !!! | GTTTTAATACTTTTTTTATG+AGG | + | chr4.1:6659580-6659599 | MS.gene70093:intron | 15.0% |
| !! | AAAGTCAAAATGAATTTCAA+AGG | - | chr4.1:6659764-6659783 | None:intergenic | 20.0% |
| !! | AACATTGCATATAATAAATG+TGG | - | chr4.1:6659101-6659120 | None:intergenic | 20.0% |
| !! | AAGTCAAAATGAATTTCAAA+GGG | - | chr4.1:6659763-6659782 | None:intergenic | 20.0% |
| !! | ACATACACATTAATATGTTA+AGG | + | chr4.1:6659362-6659381 | MS.gene70093:intron | 20.0% |
| !! | ATAAACAACAATTATCGTAT+AGG | + | chr4.1:6659443-6659462 | MS.gene70093:intron | 20.0% |
| !! | ATTTGTATTTACACTTCAAT+AGG | + | chr4.1:6658999-6659018 | MS.gene70093:intron | 20.0% |
| !! | TGAAAATAACTTATGAACAA+CGG | + | chr4.1:6659558-6659577 | MS.gene70093:intron | 20.0% |
| !!! | AAAAAATGTTACTATGCTTT+TGG | + | chr4.1:6659131-6659150 | MS.gene70093:intron | 20.0% |
| !!! | ACATTAATATGTTAAGGTTT+TGG | + | chr4.1:6659368-6659387 | MS.gene70093:intron | 20.0% |
| !!! | AGTGAAAATTTTAATTTGAG+TGG | + | chr4.1:6659612-6659631 | MS.gene70093:intron | 20.0% |
| !!! | ATTATTGGTTTTGATTTTGA+TGG | + | chr4.1:6660090-6660109 | MS.gene70093:intron | 20.0% |
| !!! | TGATTGATTCTTAATTAATG+TGG | + | chr4.1:6660191-6660210 | MS.gene70093:intron | 20.0% |
| ! | AAAAAAATATTGTACTCCGT+TGG | + | chr4.1:6660023-6660042 | MS.gene70093:intron | 25.0% |
| ! | AAAACCAATAATGTTCATTC+AGG | - | chr4.1:6660082-6660101 | None:intergenic | 25.0% |
| ! | AGAGTTTAACCTTAATATTG+AGG | + | chr4.1:6660243-6660262 | MS.gene70093:CDS | 25.0% |
| ! | AGTATATAGCATAGTTACAT+TGG | + | chr4.1:6659316-6659335 | MS.gene70093:intron | 25.0% |
| ! | ATTAAATGCATGAAATGACT+TGG | + | chr4.1:6659161-6659180 | MS.gene70093:intron | 25.0% |
| ! | CATTATCATCCTCAATATTA+AGG | - | chr4.1:6660255-6660274 | None:intergenic | 25.0% |
| ! | TATAGAGAAATTTCCAAAAG+TGG | - | chr4.1:6659211-6659230 | None:intergenic | 25.0% |
| !! | GAAACTAAATTTTCAGAACT+TGG | + | chr4.1:6659919-6659938 | MS.gene70093:CDS | 25.0% |
| !! | TTCTGAAAATTTAGTTTCTG+GGG | - | chr4.1:6659917-6659936 | None:intergenic | 25.0% |
| !!! | AATACTTTTTTTATGAGGTG+AGG | + | chr4.1:6659585-6659604 | MS.gene70093:intron | 25.0% |
| !!! | AGTTCTGAAAATTTAGTTTC+TGG | - | chr4.1:6659919-6659938 | None:intergenic | 25.0% |
| !!! | GTTCTGAAAATTTAGTTTCT+GGG | - | chr4.1:6659918-6659937 | None:intergenic | 25.0% |
| !!! | TCCAAAAGTGGTTTATTTTT+CGG | - | chr4.1:6659199-6659218 | None:intergenic | 25.0% |
| !!! | TGACTTTTAAAAAACTTGCA+AGG | + | chr4.1:6659777-6659796 | MS.gene70093:intron | 25.0% |
| !!! | TGCTTTGTTGTTTTTCTATT+TGG | + | chr4.1:6659420-6659439 | MS.gene70093:intron | 25.0% |
| CTTGTAGAGAAATTGAAAGA+GGG | - | chr4.1:6658920-6658939 | None:intergenic | 30.0% | |
| GTAAGTATATGCATTAAGAG+TGG | - | chr4.1:6658970-6658989 | None:intergenic | 30.0% | |
| GTTTGACATTTGATATGTTG+TGG | + | chr4.1:6659044-6659063 | MS.gene70093:intron | 30.0% | |
| TATAGCATAGTTACATTGGT+AGG | + | chr4.1:6659320-6659339 | MS.gene70093:intron | 30.0% | |
| TGATTCTTAATTAATGTGGC+AGG | + | chr4.1:6660195-6660214 | MS.gene70093:intron | 30.0% | |
| ! | TCCGAAAAATAAACCACTTT+TGG | + | chr4.1:6659195-6659214 | MS.gene70093:intron | 30.0% |
| !! | TCTTTCAGAACTTCAAGTTT+TGG | + | chr4.1:6659655-6659674 | MS.gene70093:CDS | 30.0% |
| !!! | TTTGATTTTGATGGTGCATT+TGG | + | chr4.1:6660099-6660118 | MS.gene70093:intron | 30.0% |
| AAACGAGAAGATCTCAAAGA+AGG | - | chr4.1:6659715-6659734 | None:intergenic | 35.0% | |
| AGTGGTTAAAACATACATGC+AGG | + | chr4.1:6659630-6659649 | MS.gene70093:intron | 35.0% | |
| CCAATTGATACTTTAGGTGT+TGG | + | chr4.1:6659286-6659305 | MS.gene70093:intron | 35.0% | |
| CCTCTTTCAATTTCTCTACA+AGG | + | chr4.1:6658918-6658937 | MS.gene70093:CDS | 35.0% | |
| CCTTGTAGAGAAATTGAAAG+AGG | - | chr4.1:6658921-6658940 | None:intergenic | 35.0% | |
| GAAATTGAAAGAGGGTTGAA+AGG | - | chr4.1:6658912-6658931 | None:intergenic | 35.0% | |
| GAACTTGACCTTATAACCAA+CGG | - | chr4.1:6660042-6660061 | None:intergenic | 35.0% | |
| GACACCTGAATGAACATTAT+TGG | + | chr4.1:6660075-6660094 | MS.gene70093:intron | 35.0% | |
| GAGTGTCCAATTGATACTTT+AGG | + | chr4.1:6659280-6659299 | MS.gene70093:intron | 35.0% | |
| GTTGTTTATGCTAGAGCTAT+GGG | + | chr4.1:6660157-6660176 | MS.gene70093:intron | 35.0% | |
| TCAAACTTACTGCACAAATG+CGG | - | chr4.1:6659738-6659757 | None:intergenic | 35.0% | |
| TTCTTAATTAATGTGGCAGG+TGG | + | chr4.1:6660198-6660217 | MS.gene70093:intron | 35.0% | |
| ! | ATTGTACTCCGTTGGTTATA+AGG | + | chr4.1:6660031-6660050 | MS.gene70093:intron | 35.0% |
| ! | CCAACACCTAAAGTATCAAT+TGG | - | chr4.1:6659289-6659308 | None:intergenic | 35.0% |
| ! | TGTTGTTTATGCTAGAGCTA+TGG | + | chr4.1:6660156-6660175 | MS.gene70093:intron | 35.0% |
| !! | AAGTTTTGGTGCAATTTCTG+TGG | + | chr4.1:6659669-6659688 | MS.gene70093:CDS | 35.0% |
| !! | AGTTTTGGTGCAATTTCTGT+GGG | + | chr4.1:6659670-6659689 | MS.gene70093:CDS | 35.0% |
| ACACGTGCAAATTAAACGCA+CGG | + | chr4.1:6659249-6659268 | MS.gene70093:intron | 40.0% | |
| GGAAGATGAAAATCTGAACC+TGG | - | chr4.1:6658949-6658968 | None:intergenic | 40.0% | |
| TTTCTCTACAAGGACTAACC+AGG | + | chr4.1:6658928-6658947 | MS.gene70093:CDS | 40.0% | |
| ! | CAAATCAGCTGCTTGTTGAA+TGG | - | chr4.1:6660293-6660312 | None:intergenic | 40.0% |
| !! | GGAAATAGTGATGGGTTTAG+AGG | + | chr4.1:6660219-6660238 | MS.gene70093:CDS | 40.0% |
| !! | TTTAGTTTCTGGGGTAAGCT+TGG | - | chr4.1:6659908-6659927 | None:intergenic | 40.0% |
| AAGAGAGGTGGTTGAAAGAG+AGG | - | chr4.1:6658888-6658907 | None:intergenic | 45.0% | |
| AATTAAACGCACGGACGTGA+AGG | + | chr4.1:6659258-6659277 | MS.gene70093:intron | 45.0% | |
| AGAGGGTTGAAAGGTAAGAG+AGG | - | chr4.1:6658903-6658922 | None:intergenic | 45.0% | |
| CATTTGATATGTTGTGGCGC+AGG | + | chr4.1:6659050-6659069 | MS.gene70093:intron | 45.0% | |
| TGGCAGGTGGAAATAGTGAT+GGG | + | chr4.1:6660211-6660230 | MS.gene70093:intron | 45.0% | |
| CTCACTTTCTCCACTGTCTC+AGG | - | chr4.1:6659847-6659866 | None:intergenic | 50.0% | |
| GGGTTGAAAGGTAAGAGAGG+TGG | - | chr4.1:6658900-6658919 | None:intergenic | 50.0% | |
| GTGGCAGGTGGAAATAGTGA+TGG | + | chr4.1:6660210-6660229 | MS.gene70093:intron | 50.0% | |
| TCAGGTGTCAGCTCAGTGAT+AGG | - | chr4.1:6660064-6660083 | None:intergenic | 50.0% | |
| ! | TCTGGGGTAAGCTTGGTTTC+AGG | - | chr4.1:6659901-6659920 | None:intergenic | 50.0% |
| TCAGGCACAACCTGAGACAG+TGG | + | chr4.1:6659834-6659853 | MS.gene70093:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 6658881 | 6660342 | 6658881 | ID=MS.gene70093 |
| chr4.1 | mRNA | 6658881 | 6660342 | 6658881 | ID=MS.gene70093.t1;Parent=MS.gene70093 |
| chr4.1 | exon | 6658881 | 6658949 | 6658881 | ID=MS.gene70093.t1.exon1;Parent=MS.gene70093.t1 |
| chr4.1 | CDS | 6658881 | 6658949 | 6658881 | ID=cds.MS.gene70093.t1;Parent=MS.gene70093.t1 |
| chr4.1 | exon | 6659652 | 6659747 | 6659652 | ID=MS.gene70093.t1.exon2;Parent=MS.gene70093.t1 |
| chr4.1 | CDS | 6659652 | 6659747 | 6659652 | ID=cds.MS.gene70093.t1;Parent=MS.gene70093.t1 |
| chr4.1 | exon | 6659832 | 6659960 | 6659832 | ID=MS.gene70093.t1.exon3;Parent=MS.gene70093.t1 |
| chr4.1 | CDS | 6659832 | 6659960 | 6659832 | ID=cds.MS.gene70093.t1;Parent=MS.gene70093.t1 |
| chr4.1 | exon | 6660217 | 6660342 | 6660217 | ID=MS.gene70093.t1.exon4;Parent=MS.gene70093.t1 |
| chr4.1 | CDS | 6660217 | 6660342 | 6660217 | ID=cds.MS.gene70093.t1;Parent=MS.gene70093.t1 |
| Gene Sequence |
| Protein sequence |
