Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70095.t1 | XP_003630571.1 | 97.1 | 139 | 4 | 0 | 1 | 139 | 1 | 139 | 2.40E-57 | 231.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70095.t1 | P52414 | 55.3 | 114 | 49 | 1 | 27 | 138 | 24 | 137 | 2.3e-24 | 113.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70095.t1 | G7LIR8 | 97.1 | 139 | 4 | 0 | 1 | 139 | 1 | 139 | 1.7e-57 | 231.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049491 | MS.gene70095 | 0.83185 | 1.32E-55 | -1.69E-46 |
| MS.gene049614 | MS.gene70095 | 0.810732 | 9.96E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene70095 | 0.805597 | 1.24E-49 | -1.69E-46 |
| MS.gene050076 | MS.gene70095 | 0.834528 | 2.84E-56 | -1.69E-46 |
| MS.gene050301 | MS.gene70095 | 0.831029 | 2.10E-55 | -1.69E-46 |
| MS.gene050767 | MS.gene70095 | 0.865679 | 4.96E-65 | -1.69E-46 |
| MS.gene050770 | MS.gene70095 | 0.86605 | 3.78E-65 | -1.69E-46 |
| MS.gene05101 | MS.gene70095 | 0.861238 | 1.18E-63 | -1.69E-46 |
| MS.gene051471 | MS.gene70095 | 0.843248 | 1.58E-58 | -1.69E-46 |
| MS.gene052078 | MS.gene70095 | 0.842487 | 2.51E-58 | -1.69E-46 |
| MS.gene052290 | MS.gene70095 | 0.823051 | 1.71E-53 | -1.69E-46 |
| MS.gene052293 | MS.gene70095 | 0.801788 | 7.63E-49 | -1.69E-46 |
| MS.gene052326 | MS.gene70095 | 0.881236 | 2.84E-70 | -1.69E-46 |
| MS.gene053322 | MS.gene70095 | 0.817354 | 3.44E-52 | -1.69E-46 |
| MS.gene054376 | MS.gene70095 | 0.871707 | 5.57E-67 | -1.69E-46 |
| MS.gene05455 | MS.gene70095 | 0.814371 | 1.59E-51 | -1.69E-46 |
| MS.gene054802 | MS.gene70095 | 0.84759 | 1.05E-59 | -1.69E-46 |
| MS.gene054803 | MS.gene70095 | 0.850312 | 1.84E-60 | -1.69E-46 |
| MS.gene054804 | MS.gene70095 | 0.849864 | 2.46E-60 | -1.69E-46 |
| MS.gene055254 | MS.gene70095 | 0.848419 | 6.20E-60 | -1.69E-46 |
| MS.gene055277 | MS.gene70095 | 0.832573 | 8.75E-56 | -1.69E-46 |
| MS.gene055390 | MS.gene70095 | 0.84191 | 3.57E-58 | -1.69E-46 |
| MS.gene055397 | MS.gene70095 | 0.836497 | 9.04E-57 | -1.69E-46 |
| MS.gene055422 | MS.gene70095 | 0.849293 | 3.55E-60 | -1.69E-46 |
| MS.gene055428 | MS.gene70095 | 0.803958 | 2.72E-49 | -1.69E-46 |
| MS.gene055499 | MS.gene70095 | 0.848386 | 6.34E-60 | -1.69E-46 |
| MS.gene055896 | MS.gene70095 | 0.833867 | 4.17E-56 | -1.69E-46 |
| MS.gene057123 | MS.gene70095 | 0.822416 | 2.40E-53 | -1.69E-46 |
| MS.gene057129 | MS.gene70095 | 0.820245 | 7.59E-53 | -1.69E-46 |
| MS.gene057295 | MS.gene70095 | 0.807061 | 6.08E-50 | -1.69E-46 |
| MS.gene058293 | MS.gene70095 | 0.804783 | 1.83E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene70095 | 0.878807 | 2.08E-69 | -1.69E-46 |
| MS.gene059228 | MS.gene70095 | 0.821939 | 3.09E-53 | -1.69E-46 |
| MS.gene059326 | MS.gene70095 | 0.820602 | 6.29E-53 | -1.69E-46 |
| MS.gene059597 | MS.gene70095 | 0.828234 | 1.01E-54 | -1.69E-46 |
| MS.gene059960 | MS.gene70095 | 0.831702 | 1.44E-55 | -1.69E-46 |
| MS.gene060005 | MS.gene70095 | 0.812412 | 4.30E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene70095 | 0.811871 | 5.64E-51 | -1.69E-46 |
| MS.gene060559 | MS.gene70095 | 0.800058 | 1.72E-48 | -1.69E-46 |
| MS.gene061200 | MS.gene70095 | 0.830246 | 3.27E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70095.t1 | MTR_8g099920 | 97.122 | 139 | 4 | 0 | 1 | 139 | 1 | 139 | 2.61e-95 | 271 |
| MS.gene70095.t1 | MTR_8g099920 | 95.683 | 139 | 4 | 1 | 1 | 139 | 1 | 137 | 6.45e-92 | 262 |
| MS.gene70095.t1 | MTR_8g099920 | 95.000 | 100 | 5 | 0 | 1 | 100 | 1 | 100 | 6.72e-64 | 191 |
| MS.gene70095.t1 | MTR_2g020360 | 47.552 | 143 | 65 | 4 | 1 | 138 | 1 | 138 | 4.10e-38 | 126 |
| MS.gene70095.t1 | MTR_2g020360 | 46.853 | 143 | 66 | 4 | 1 | 138 | 1 | 138 | 3.05e-36 | 121 |
| MS.gene70095.t1 | MTR_2g020360 | 51.220 | 123 | 52 | 4 | 22 | 138 | 18 | 138 | 5.29e-34 | 115 |
| MS.gene70095.t1 | MTR_4g118750 | 46.535 | 101 | 35 | 3 | 42 | 138 | 37 | 122 | 3.45e-21 | 83.6 |
| MS.gene70095.t1 | MTR_1g081920 | 50.000 | 80 | 35 | 2 | 56 | 133 | 10 | 86 | 7.93e-18 | 73.6 |
| MS.gene70095.t1 | MTR_7g080760 | 45.833 | 72 | 37 | 2 | 61 | 131 | 42 | 112 | 1.73e-11 | 57.8 |
| MS.gene70095.t1 | MTR_7g080390 | 41.772 | 79 | 44 | 2 | 61 | 138 | 53 | 130 | 4.83e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70095.t1 | AT4G25050 | 56.115 | 139 | 52 | 4 | 1 | 138 | 1 | 131 | 5.38e-40 | 131 |
| MS.gene70095.t1 | AT4G25050 | 51.656 | 151 | 52 | 5 | 1 | 138 | 1 | 143 | 1.49e-36 | 122 |
| MS.gene70095.t1 | AT3G05020 | 50.000 | 136 | 52 | 4 | 4 | 138 | 16 | 136 | 2.84e-34 | 116 |
| MS.gene70095.t1 | AT5G27200 | 58.163 | 98 | 38 | 2 | 42 | 138 | 42 | 137 | 1.20e-31 | 110 |
| MS.gene70095.t1 | AT1G54630 | 43.137 | 153 | 53 | 7 | 1 | 138 | 1 | 134 | 2.51e-28 | 101 |
| MS.gene70095.t1 | AT1G54580 | 57.647 | 85 | 34 | 1 | 54 | 138 | 52 | 134 | 1.13e-26 | 97.4 |
| MS.gene70095.t1 | AT1G65290 | 41.772 | 79 | 44 | 2 | 61 | 138 | 49 | 126 | 1.67e-11 | 58.2 |
| MS.gene70095.t1 | AT1G54630 | 53.448 | 58 | 25 | 1 | 54 | 111 | 52 | 107 | 3.12e-11 | 57.0 |
Find 24 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTCTGAAAATTTAGTTTC+TGG | 0.232292 | 4.1:-6701564 | None:intergenic |
| TCTGGGGTAAGCTTGGTTTC+AGG | 0.264840 | 4.1:-6701546 | None:intergenic |
| CATTATCATCCTCAATATTA+AGG | 0.281641 | 4.1:-6701900 | None:intergenic |
| CTCACTTTCTCCACTGTCTC+AGG | 0.295443 | 4.1:-6701492 | None:intergenic |
| TCTTTCAGAACTTCAAGTTT+TGG | 0.316075 | 4.1:+6701303 | MS.gene70095:CDS |
| GTTCTGAAAATTTAGTTTCT+GGG | 0.362213 | 4.1:-6701563 | None:intergenic |
| GGAAATAGTGATGGGTTTAG+AGG | 0.377032 | 4.1:+6701867 | MS.gene70095:CDS |
| TTTAGTTTCTGGGGTAAGCT+TGG | 0.464049 | 4.1:-6701553 | None:intergenic |
| CAAATCAGCTGCTTGTTGAA+TGG | 0.477053 | 4.1:-6701938 | None:intergenic |
| CCTCTTTCAATTTCTCTACA+AGG | 0.482096 | 4.1:+6700566 | MS.gene70095:CDS |
| AGAGTTTAACCTTAATATTG+AGG | 0.511328 | 4.1:+6701891 | MS.gene70095:CDS |
| GAAATTGAAAGAGGGTTGAA+AGG | 0.517599 | 4.1:-6700557 | None:intergenic |
| TTCTGAAAATTTAGTTTCTG+GGG | 0.526211 | 4.1:-6701562 | None:intergenic |
| CCTTGTAGAGAAATTGAAAG+AGG | 0.526756 | 4.1:-6700566 | None:intergenic |
| AGAGGGTTGAAAGGTAAGAG+AGG | 0.538850 | 4.1:-6700548 | None:intergenic |
| AAGAGAGGTGGTTGAAAGAG+AGG | 0.539433 | 4.1:-6700533 | None:intergenic |
| AAACGAGAAGATCTCAAAGA+AGG | 0.566334 | 4.1:-6701360 | None:intergenic |
| GGGTTGAAAGGTAAGAGAGG+TGG | 0.569945 | 4.1:-6700545 | None:intergenic |
| GTGGCAGGTGGAAATAGTGA+TGG | 0.572304 | 4.1:+6701858 | MS.gene70095:intron |
| CTTGTAGAGAAATTGAAAGA+GGG | 0.583035 | 4.1:-6700565 | None:intergenic |
| TCAGGCACAACCTGAGACAG+TGG | 0.583358 | 4.1:+6701482 | MS.gene70095:CDS |
| TTTCTCTACAAGGACTAACC+AGG | 0.601936 | 4.1:+6700576 | MS.gene70095:CDS |
| TGGCAGGTGGAAATAGTGAT+GGG | 0.642802 | 4.1:+6701859 | MS.gene70095:intron |
| TCAAACTTACTGCACAAATG+CGG | 0.672194 | 4.1:-6701383 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTTTTTTAAAAGATTC+AGG | + | chr4.1:6701464-6701483 | MS.gene70095:intron | 10.0% |
| !!! | GTTTTAATACTTTTTTTATG+AGG | + | chr4.1:6701228-6701247 | MS.gene70095:intron | 15.0% |
| !! | AAAGTCAAAATGAATTTCAA+AGG | - | chr4.1:6701412-6701431 | None:intergenic | 20.0% |
| !! | AACATTGCATATAATAAATG+TGG | - | chr4.1:6700749-6700768 | None:intergenic | 20.0% |
| !! | AAGTCAAAATGAATTTCAAA+GGG | - | chr4.1:6701411-6701430 | None:intergenic | 20.0% |
| !! | ACATACACATTAATATGTTA+AGG | + | chr4.1:6701010-6701029 | MS.gene70095:intron | 20.0% |
| !! | ATAAACAACAATTATCGTAT+AGG | + | chr4.1:6701091-6701110 | MS.gene70095:intron | 20.0% |
| !! | ATTTGTATTTACACTTCAAT+AGG | + | chr4.1:6700647-6700666 | MS.gene70095:intron | 20.0% |
| !! | TGAAAATAACTTATGAACAA+CGG | + | chr4.1:6701206-6701225 | MS.gene70095:intron | 20.0% |
| !!! | AAAAAATGTTACTATGCTTT+TGG | + | chr4.1:6700779-6700798 | MS.gene70095:intron | 20.0% |
| !!! | ACATTAATATGTTAAGGTTT+TGG | + | chr4.1:6701016-6701035 | MS.gene70095:intron | 20.0% |
| !!! | AGTGAAAATTTTAATTTGAG+TGG | + | chr4.1:6701260-6701279 | MS.gene70095:intron | 20.0% |
| !!! | ATTATTGGTTTTGATTTTGA+TGG | + | chr4.1:6701738-6701757 | MS.gene70095:intron | 20.0% |
| !!! | TGATTGATTCTTAATTAATG+TGG | + | chr4.1:6701839-6701858 | MS.gene70095:intron | 20.0% |
| ! | AAAAAAATATTGTACTCCGT+TGG | + | chr4.1:6701671-6701690 | MS.gene70095:intron | 25.0% |
| ! | AAAACCAATAATGTTCATTC+AGG | - | chr4.1:6701730-6701749 | None:intergenic | 25.0% |
| ! | AGAGTTTAACCTTAATATTG+AGG | + | chr4.1:6701891-6701910 | MS.gene70095:CDS | 25.0% |
| ! | AGTATATAGCATAGTTACAT+TGG | + | chr4.1:6700964-6700983 | MS.gene70095:intron | 25.0% |
| ! | ATTAAATGCATGAAATGACT+TGG | + | chr4.1:6700809-6700828 | MS.gene70095:intron | 25.0% |
| ! | CATTATCATCCTCAATATTA+AGG | - | chr4.1:6701903-6701922 | None:intergenic | 25.0% |
| ! | TATAGAGAAATTTCCAAAAG+TGG | - | chr4.1:6700859-6700878 | None:intergenic | 25.0% |
| !! | GAAACTAAATTTTCAGAACT+TGG | + | chr4.1:6701567-6701586 | MS.gene70095:CDS | 25.0% |
| !! | TTCTGAAAATTTAGTTTCTG+GGG | - | chr4.1:6701565-6701584 | None:intergenic | 25.0% |
| !!! | AATACTTTTTTTATGAGGTG+AGG | + | chr4.1:6701233-6701252 | MS.gene70095:intron | 25.0% |
| !!! | AGTTCTGAAAATTTAGTTTC+TGG | - | chr4.1:6701567-6701586 | None:intergenic | 25.0% |
| !!! | GTTCTGAAAATTTAGTTTCT+GGG | - | chr4.1:6701566-6701585 | None:intergenic | 25.0% |
| !!! | TCCAAAAGTGGTTTATTTTT+CGG | - | chr4.1:6700847-6700866 | None:intergenic | 25.0% |
| !!! | TGACTTTTAAAAAACTTGCA+AGG | + | chr4.1:6701425-6701444 | MS.gene70095:intron | 25.0% |
| !!! | TGCTTTGTTGTTTTTCTATT+TGG | + | chr4.1:6701068-6701087 | MS.gene70095:intron | 25.0% |
| CTTGTAGAGAAATTGAAAGA+GGG | - | chr4.1:6700568-6700587 | None:intergenic | 30.0% | |
| GTAAGTATATGCATTAAGAG+TGG | - | chr4.1:6700618-6700637 | None:intergenic | 30.0% | |
| GTTTGACATTTGATATGTTG+TGG | + | chr4.1:6700692-6700711 | MS.gene70095:intron | 30.0% | |
| TATAGCATAGTTACATTGGT+AGG | + | chr4.1:6700968-6700987 | MS.gene70095:intron | 30.0% | |
| TGATTCTTAATTAATGTGGC+AGG | + | chr4.1:6701843-6701862 | MS.gene70095:intron | 30.0% | |
| ! | TCCGAAAAATAAACCACTTT+TGG | + | chr4.1:6700843-6700862 | MS.gene70095:intron | 30.0% |
| !! | TCTTTCAGAACTTCAAGTTT+TGG | + | chr4.1:6701303-6701322 | MS.gene70095:CDS | 30.0% |
| !!! | TTTGATTTTGATGGTGCATT+TGG | + | chr4.1:6701747-6701766 | MS.gene70095:intron | 30.0% |
| AAACGAGAAGATCTCAAAGA+AGG | - | chr4.1:6701363-6701382 | None:intergenic | 35.0% | |
| AGTGGTTAAAACATACATGC+AGG | + | chr4.1:6701278-6701297 | MS.gene70095:intron | 35.0% | |
| CCAATTGATACTTTAGGTGT+TGG | + | chr4.1:6700934-6700953 | MS.gene70095:intron | 35.0% | |
| CCTCTTTCAATTTCTCTACA+AGG | + | chr4.1:6700566-6700585 | MS.gene70095:CDS | 35.0% | |
| CCTTGTAGAGAAATTGAAAG+AGG | - | chr4.1:6700569-6700588 | None:intergenic | 35.0% | |
| GAAATTGAAAGAGGGTTGAA+AGG | - | chr4.1:6700560-6700579 | None:intergenic | 35.0% | |
| GAACTTGACCTTATAACCAA+CGG | - | chr4.1:6701690-6701709 | None:intergenic | 35.0% | |
| GACACCTGAATGAACATTAT+TGG | + | chr4.1:6701723-6701742 | MS.gene70095:intron | 35.0% | |
| GAGTGTCCAATTGATACTTT+AGG | + | chr4.1:6700928-6700947 | MS.gene70095:intron | 35.0% | |
| GTTGTTTATGCTAGAGCTAT+GGG | + | chr4.1:6701805-6701824 | MS.gene70095:intron | 35.0% | |
| TCAAACTTACTGCACAAATG+CGG | - | chr4.1:6701386-6701405 | None:intergenic | 35.0% | |
| TTCTTAATTAATGTGGCAGG+TGG | + | chr4.1:6701846-6701865 | MS.gene70095:intron | 35.0% | |
| ! | ATTGTACTCCGTTGGTTATA+AGG | + | chr4.1:6701679-6701698 | MS.gene70095:intron | 35.0% |
| ! | CCAACACCTAAAGTATCAAT+TGG | - | chr4.1:6700937-6700956 | None:intergenic | 35.0% |
| ! | TGTTGTTTATGCTAGAGCTA+TGG | + | chr4.1:6701804-6701823 | MS.gene70095:intron | 35.0% |
| !! | AAGTTTTGGTGCAATTTCTG+TGG | + | chr4.1:6701317-6701336 | MS.gene70095:CDS | 35.0% |
| !! | AGTTTTGGTGCAATTTCTGT+GGG | + | chr4.1:6701318-6701337 | MS.gene70095:CDS | 35.0% |
| ACACGTGCAAATTAAACGCA+CGG | + | chr4.1:6700897-6700916 | MS.gene70095:intron | 40.0% | |
| GGAAGATGAAAATCTGAACC+TGG | - | chr4.1:6700597-6700616 | None:intergenic | 40.0% | |
| TTTCTCTACAAGGACTAACC+AGG | + | chr4.1:6700576-6700595 | MS.gene70095:CDS | 40.0% | |
| ! | CAAATCAGCTGCTTGTTGAA+TGG | - | chr4.1:6701941-6701960 | None:intergenic | 40.0% |
| !! | GGAAATAGTGATGGGTTTAG+AGG | + | chr4.1:6701867-6701886 | MS.gene70095:CDS | 40.0% |
| !! | TTTAGTTTCTGGGGTAAGCT+TGG | - | chr4.1:6701556-6701575 | None:intergenic | 40.0% |
| AAGAGAGGTGGTTGAAAGAG+AGG | - | chr4.1:6700536-6700555 | None:intergenic | 45.0% | |
| AATTAAACGCACGGACGTGA+AGG | + | chr4.1:6700906-6700925 | MS.gene70095:intron | 45.0% | |
| AGAGGGTTGAAAGGTAAGAG+AGG | - | chr4.1:6700551-6700570 | None:intergenic | 45.0% | |
| CATTTGATATGTTGTGGCGC+AGG | + | chr4.1:6700698-6700717 | MS.gene70095:intron | 45.0% | |
| TGGCAGGTGGAAATAGTGAT+GGG | + | chr4.1:6701859-6701878 | MS.gene70095:intron | 45.0% | |
| CTCACTTTCTCCACTGTCTC+AGG | - | chr4.1:6701495-6701514 | None:intergenic | 50.0% | |
| GGGTTGAAAGGTAAGAGAGG+TGG | - | chr4.1:6700548-6700567 | None:intergenic | 50.0% | |
| GTGGCAGGTGGAAATAGTGA+TGG | + | chr4.1:6701858-6701877 | MS.gene70095:intron | 50.0% | |
| TCAGGTGTCAGCTCAGTGAT+AGG | - | chr4.1:6701712-6701731 | None:intergenic | 50.0% | |
| ! | TCTGGGGTAAGCTTGGTTTC+AGG | - | chr4.1:6701549-6701568 | None:intergenic | 50.0% |
| TCAGGCACAACCTGAGACAG+TGG | + | chr4.1:6701482-6701501 | MS.gene70095:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 6700529 | 6701990 | 6700529 | ID=MS.gene70095 |
| chr4.1 | mRNA | 6700529 | 6701990 | 6700529 | ID=MS.gene70095.t1;Parent=MS.gene70095 |
| chr4.1 | exon | 6700529 | 6700597 | 6700529 | ID=MS.gene70095.t1.exon1;Parent=MS.gene70095.t1 |
| chr4.1 | CDS | 6700529 | 6700597 | 6700529 | ID=cds.MS.gene70095.t1;Parent=MS.gene70095.t1 |
| chr4.1 | exon | 6701300 | 6701395 | 6701300 | ID=MS.gene70095.t1.exon2;Parent=MS.gene70095.t1 |
| chr4.1 | CDS | 6701300 | 6701395 | 6701300 | ID=cds.MS.gene70095.t1;Parent=MS.gene70095.t1 |
| chr4.1 | exon | 6701480 | 6701608 | 6701480 | ID=MS.gene70095.t1.exon3;Parent=MS.gene70095.t1 |
| chr4.1 | CDS | 6701480 | 6701608 | 6701480 | ID=cds.MS.gene70095.t1;Parent=MS.gene70095.t1 |
| chr4.1 | exon | 6701865 | 6701990 | 6701865 | ID=MS.gene70095.t1.exon4;Parent=MS.gene70095.t1 |
| chr4.1 | CDS | 6701865 | 6701990 | 6701865 | ID=cds.MS.gene70095.t1;Parent=MS.gene70095.t1 |
| Gene Sequence |
| Protein sequence |
